INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
·
IF 8.7
Qi Wang,Jianfu Qiao,Junchen Zhou,Shengpeng Li,Biyang Ling,Hong Gu,Ziru Dai
Mature strawberries are highly susceptible to mechanical damage and microbial contamination, leading to rapid deterioration during postharvest handling and retail distribution. In this study, a functional resveratrol nanoemulsion (RM)/carrageenan composite preservation film (C-RM) was developed for strawberry preservation. The prepared nanoemulsion exhibited good dispersion stability with an average particle size of 73 nm, a PDI of 0.28, and a zeta potential of −33.16 mV, enabling controlled release within the film matrix. At an RM loading of 6% (C-RM 3 ), the tensile strength of the film increased from 39.97 MPa (control) to 49.19 MPa, accompanied by enhanced UV-blocking performance. FTIR and XRD analyses indicated that the nanoemulsion interacted with carrageenan through hydrogen bonding rather than covalent bonding, resulting in reduced crystallinity of the polymer matrix. The C-RM 3 film exhibited strong antibacterial activity, achieving inhibition rates of 97.6% against Escherichia coli ( E. coli ) and 95.9% against Staphylococcus aureus ( S. aureus ), while antioxidant capacity was significantly improved, with DPPH and ABTS radical scavenging activities reaching 72.5% and 87.8%, respectively. Toxicity evaluations using mice and zebrafish confirmed good biosafety. After 5 days of storage at room temperature, strawberries packaged with C-RM 3 maintained superior color, firmness, titratable acidity, and soluble solid content compared with the control, extending shelf life by 2 days. These results demonstrate that the C-RM film exhibits excellent antibacterial, antioxidant, and preservation performance, highlighting its potential as a sustainable green packaging material for fresh produce.
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高影响因子
Antioxidants
·
2026
·Vol. 15
·期 9
·1096
·
IF 8.2
Juexi Liu,Lingtong Fan,Jingran Wei,Qingsong Liu,Ouyan Han,Yan Yang,Danjun Guo,Wei Xu,Huajuan Wang,E Liao
Population aging has made sarcopenia a growing concern in geriatric health. Protein–polyphenol non-covalent complexes can serve as carrier systems that improve the stability and bioactivity of natural antioxidants. This study refined the preparation parameters for the non-covalent complex of whey protein isolate (WPI) and resveratrol (RES), achieving protein digestibility of 83.60 ± 0.50% and DPPH scavenging of 52.41 ± 0.58% at pH 7.0, a WPI:RES molar ratio of 1:1, and a reaction time of 1.5 h. Relative to free WPI, the complex improved DPPH scavenging by 21.66% while preserving protein digestibility. The binding mode, interaction forces, and conformational evolution were investigated via spectroscopic experiments, docking studies and molecular dynamics simulations. RES selectively bound to the surface of β-lactoglobulin in WPI through hydrophobic interactions and hydrogen bonding, with a docking score of −6.366 kcal/mol and an MM-GBSA binding free energy of −30.48 kcal/mol. The complex maintained a highly stable conformation throughout the 100 ns molecular dynamics simulation. In conclusion, this study elucidated the structural changes of WPI upon non-covalent resveratrol binding. The WPI-RES complex exhibited enhanced DPPH radical scavenging activity while preserving protein digestibility, with potential implications for functional food development in sarcopenia management.
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高影响因子
Foods
·
2022
·Vol. 12
·期 1
·94
·
IF 5.561
Jingling Guo,Pan Wang,Yifan Cui,Xiaosong Hu,Fang Chen,Chen Ma
Resveratrol (RSV), a polyphenolic stilbene, has been widely studied for its protective effects against non-alcoholic fatty liver disease (NAFLD) by modulating intestinal microbiota. The microbial metabolites after RSV supplement would contribute to the bioeffects of RSV, while their impacts on NAFLD were unclear. Therefore, this study aimed to investigate the beneficial effects of the main microbial metabolites from RSV on lipid metabolism by combining in vitro and in vivo models. The mice were fed a high-fat diet and injected with RSV, 3-hydroxyphenyl propionic acid (3-HPP), and 4-HPP for 13 weeks (n= 6). Body weight, serum parameters, histological analysis, and gene expression involved in lipid metabolism were quantified. Our results suggested that 100 μM of 3-HPP and 4-HPP inhibited lipid accumulation more significantly than parent RSV in an oleic acid-induced HepG2 cell line. Furthermore, 3-HPP, 4-HPP, and RSV effectively reduced liver weight and body weight, improved hepatic steatosis, and alleviated systemic inflammation in NAFLD mice. In addition, the results of quantitative real-time PCR showed that 3-HPP and 4-HPP altered the expression of cholesterol influx and efflux genes to a stronger extent than RSV. These results indicate that 3-HPP and 4-HPP are effective in regulating hepatic lipid metabolism.Keywords:NAFLD;microbial metabolites;3-hydroxyphenyl propionic acid;4-hydroxyphenyl propionic acid;lipid metabolism
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Wenhao Li,Decheng Bi,Jiang Yi,Lijun Yao,Jue Cao,Peng Yang,Meiting Li,Yan Wu,Hong Xu,Zhangli Hu,Xu Xu
Polyguluronate (PG) is an acidic homopolymer of α-(1,4)- l -guluronate separated from alginate. In this study, PG was first grafted with soy protein isolate (SPI) through the Maillard reaction to obtain a new glycoprotein (SPI-PG). Then, this novel glycoprotein was used to prepare nanoparticles to encapsulate the resveratrol (RES). Our results showed that SPI-PG-RES had better pH stability, storage stability and ionic stability than SPI-RES. In vitro digestion experiments showed that the RES bioavailability of SPI-PG-RES was much higher than that of free RES and SPI-RES. Furthermore, the in vitro antioxidant capacity of SPI-PG-RES was much stronger than that of free RES and SPI-RES. In addition, SPI-PG-RES was more effective in preventing the symptoms of DSS-induced colitis than RES and SPI-RES. These results suggested that the protein nanoparticles prepared using SPI-PG were a stable and effective hydrophobic polyphenol carrier and could be applied to food-grade components in functional foods and nutritional supplements.
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JOURNAL OF FOOD ENGINEERING
·
2022
·
IF 6.203
Zhongyu Yang,David Julian McClements,Xinwen Peng,Zhenlin Xu,Man Meng,Long Chen,Zhengyu Jin
Composite nanoparticles composed of zein and carboxymethyl cellulose (CMC) designed for the co-delivery of resveratrol and quercetin were prepared using antisolvent precipitation. At a mass ratio of zein-to-CMC of 2:1, composite nanoparticles with small average diameters (217 nm) could be formed. These nanoparticles had good thermal stability and did not aggregate or sediment when stored at pH 3.0–6.0. Encapsulation of quercetin and resveratrol simultaneously within the composite nanoparticles significantly improved their antioxidant activities . This study suggests that composite nanoparticles may have good physical and chemical stability, which may increase their application as co-delivery systems for bioactive components. As a result, they may be useful for personalized nutritional applications where combinations of bioactive compounds need to be orally administered.
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Materials Today Bio
·
2021
·
IF 7.348
Xue Jiang,Xiaoyao Liu,Qi Yu,Wenwen Shen,Xifan Mei,He Tian,Chao Wu
Spinal cord injury (SCI) causes secondary injury, accompanied by pathological changes such as oxidative stress, inflammation and neuronal apoptosis. This leads to permanent disabilities such as paralysis and loss of movement or sensation. Due to the ineffectiveness of drugs passing through the blood spinal cord barrier (BSCB), there is currently no effective treatment for SCI. The aim of this experiment was to design plasma complex component functionalized manganese-doped silica nanoparticles (PMMSN) with a redox response as a targeted drug carrier for resveratrol (RES), which effectively transports insoluble drugs to cross the BSCB. RES was adsorbed into PMMSN with a particle size of approximately 110 nm by the adsorption method, and the drug loading reached 32.61 ± 3.38%. The RES release results for the loaded sample (PMMSN-RES) showed that the PMMSN-RES exhibited a release slowly effect. In vitro and vivo experiments demonstrated that PMMSN-RES decreased reactive oxygen species (ROS) and malondialdehyde (MDA), increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, reduced the expression of inflammatory (TNF-α, IL-1β and IL-6) and apoptotic cytokines (cleaved caspase-3) in spinal cord tissue after SCI. In summary, PMMSN-RES may be a potential pharmaceutical preparation for the treatment of SCI by reducing neuronal apoptosis and inhibiting inflammation caused by reducing oxidative stress to promote the recovery of mouse motor function.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Hongniu Zhou,Qingyun Wang,Li Ni,Wen Zhang
8 μg/mL resveratrol suppresses spoilage potential without affecting growth. • Resveratrol causes apparent flagellar damage in S. putrefaciens JR-6. • Anti-spoilage effects persist after 30 serial passages with resveratrol. • Resveratrol-passaged JR-6 reduces TVB-N and spoilage volatiles in fish fillets. • Transcriptomics and docking suggest multi-target effects of resveratrol. Shewanella putrefaciens is a specific spoilage organism with strong spoilage potential in aquatic products. At a subinhibitory concentration of 8 μg/mL (1/8 MIC), resveratrol significantly reduced adhesion, biofilm formation, swimming motility, and extracellular protease production ability in S. putrefaciens JR-6 without affecting bacterial growth or membrane integrity. These inhibitory effects persisted after 30 serial passages. Transmission electron microscopy revealed shortened and apparently broken flagella in resveratrol-exposed cells. In fish fillet assays, inoculation with resveratrol-passaged strains delayed TVB-N accumulation and markedly reduced the semi-quantitative levels of three characteristic spoilage-associated volatile compounds. Transcriptomic analysis identified 76 differentially expressed genes associated with amino acid and nitrogen metabolism, oxidative phosphorylation, flagellar assembly, biofilm formation, and two-component systems. RT-qPCR confirmed significant downregulation of genes involved in histidine metabolism ( hisB and hisC ), nitrogen metabolism ( gltD and gltB ), oxidative phosphorylation ( coxA and coxC ), two-component systems ( dctM and dctP ), and quorum sensing ( gene3325 ). Molecular docking predicted interactions between resveratrol and the corresponding proteins through hydrogen bonding and hydrophobic contacts. These findings provide insight into the multi-target modulation of spoilage-associated phenotypes by low-concentration resveratrol and support its further evaluation as a natural preservative for aquatic products.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2016
·
IF 3.902
Jialing Pan,Tingting Xu,Fengli Xu,Yali Zhang,Zhiguo Liu,Wenbo Chen,Weitao Fu,Yuanrong Dai,Yunjie Zhao,Jianpeng Feng,Guang Liang
Acute lung injury (ALI) is a major cause of acute respiratory failure in critically-ill patients. Resveratrol and curcumin are proven to have potent anti-inflammatory efficacy, but their clinical application is limited by their metabolic instability. Here, a series of resveratrol and the Mono-carbonyl analogs of curcumin (MCAs) hybrids were designed and synthesized by efficient aldol construction strategy, and then screened for anti-inflammatory activities in vitro and in vivo . The results showed that the majority of analogs effectively inhibited the LPS-induced production of IL-6 and TNF-α. Five analogs, a9, a18, a19 , a20 and a24 exhibited excellent anti-inflammatory activity in a dose-dependent manner along with low toxicity in vitro . Structure activity relationship study revealed that the electron-withdrawing groups at meta-position and methoxyl group ( OCH 3 ) at the para position of the phenyl ring were important for anti-inflammatory activities. The most promising compound a18 decreased LPS induced TNF-α, IL-6, IL-12, and IL-33 mRNA expression. Additionally, a18 significantly protected against LPS-induced acute lung injury in the in vivo mouse model. The research of resveratrol and MCAs hybrids could bring insight into the treatment of inflammatory diseases and compound a18 may serve as a lead compound for the development of anti-ALI agents.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·
IF 5
Zhang Xiaohan,Zhang Dong,Ding Yan,Li Zilong,Wang Chenchen,Ye Shuhong
Abstract Resveratrol (RES) is a secondary metabolite synthesized by plants in response to environmental stress and pathogen infection, which is of great significance for the industrial production of RES by fermentation culture. In this study, we aimed to explore the biosynthesis pathway of RES and its key enzymes in the Priestia megaterium PH3, which was isolated and screened from peanut fruit. Through Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, we quantified the RES content and distribution in the culture medium and determined that Priestia megaterium PH3 mainly secreted RES extracellularly. Furthermore, the highest production of RES was observed in YPD, yielding an impressive 127.46 ± 6.11 μg/L. By optimizing the fermentation conditions, we achieved a remarkable RES yield of 946.82 ± 24.74 μg/L within just 2 days, which represents the highest reported yield for a natural isolate produced in such a short time frame. Our investigation revealed that the phenylpropane pathway is responsible for RES synthesis in this bacterium, with cinnamate 4-hydroxylase (C4H) identified as the main rate-limiting enzyme. Overall, our findings highlight the robust RES production capabilities of Priestia megaterium PH3, offering novel insights and potential applications for bacterial fermentation in RES production. Key points
• RES synthesized by the bacterium was confirmed through the phenylpropane pathway.
• The key rate-limiting enzyme for biosynthesis-RES is C4H.
• RES reached 946.82 ± 24.74 μg/L after fermentation for 2 days.
Graphical Abstract
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2021
·
IF 3.981
Ying-Ying Xu,Yun-Feng Huo,Lin Xu,Yu-Zhu Zhu,Yun-Tao Wu,Xiao-Yi Wei,Tao Zhou
Resveratrol (RES) possesses a variety of health-promoting effects. However, the application of RES in food and pharmaceutical industries is limited due to the poor water solubility and bioavailability, and low photostability. In order to overcome the drawback of RES, in the present work, RES-loaded nanoparticles were prepared using ovalbumin (OVA) and Porphyra haitanensis polysaccharide (PHP) as encapsulating materials. Response surface methodology was employed to optimize the fabrication of OVA-PHP-RES nanoparticles (OPR). It was found that OVA-PHP possessed superior effects on both stability of the dispersion and RES protection to those of OVA alone. In vitro simulated digestion tests demonstrated that RES in OPR was released slower in stomach and more rapidly in intestine when compared to OVA-RES nanoparticles (OR). In addition, OPR was found to effectively inhibit the growth of two tumor cell lines (HeLa and HepG2), indicating that OPR nanoparticles could deliver effectively RES in biological system, thereby improving its bioavailability. The finding in this study demonstrated OVA-PHP system could improve the stability and bioavailability of RES.
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高影响因子
MOLECULES
·
2020
·Vol. 25
·期 12
·2752
·
IF 3.267
Lili Qin,Yiwei He,Xinyu Zhao,Ting Zhang,Yao Qin,Ai Du
Silica aerogel, a kind of nanoporous material, is regarded as a desired drug carrier for its low toxicity, high specific surface area, and excellent biocompatibility. Using silica aerogel in a drug carrier may be an appropriate method to improve the performance of pure resveratrol. In this study, resveratrol-loaded silica aerogel (RSA) as a drug delivery system was prepared by the sol-gel method. Before gelling, resveratrol was added into the hydrolyzed tetraethyl orthosilicate (TEOS) ethanol solution then dispersed by stir and ultrasound. The results showed that RSA has a high loading rate of 19% with low cost and excellent biocompatibility. The SEM images showed that silica aerogel wraps up outside the resveratrol. Sustained releasing effect could be observed in RSA after 1 h, while pure resveratrol did not display this. The release of RSA lasted for over 6 h, and the release amount reached over 90% and 80% in either simulated gastric fluid (pH = 2.0) or phosphate-buffered saline (pH = 7.4) at 37 °C. Preliminary in vitro toxicity test revealed RSA to be biocompatible and stable; and when coupled with the anti-inflammatory effects of resveratrol, showed good potential for osteoarthritis treatment.Keywords:resveratrol;silica aerogel;drug carrier;characterization;release kinetics
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Food & Function
·
2018
·Vol. 9
·期 7
·3788-3797
·
IF 3.289
Wenfei Xiong,Cong Ren,Jing Li,Bin Li
The objective of this study was to investigate the impact of encapsulating resveratrol in ovalbumin (OVA)–carboxymethylcellulose (CMC) nanocomplexes or nanoparticles on its photostability and bioaccessibility. The nanocomplexes were formed by the electrostatic assembly between OVA and CMC, and the nanoparticles were fabricated through further heating the nanocomplexes at 90 °C for 30 min. The nanocomplexes and nanoparticles both presented a spherical morphology, and the nanoparticles exhibited larger average particle sizes and net zeta potential. The encapsulation efficiency and loading capacity were around 26% and 13 μg mg−1 for OVA/CMC nanocomplexes, and evidently increased to around 70% and 35 μg mg−1 for OVA/CMC nanoparticles, respectively. The major driving forces for resveratrol encapsulation were hydrogen bonding and hydrophobic interaction. Both the formulations could improve the photostability of trans-resveratrol when exposed to UV light, and the nanoparticles were more effective. Moreover, the release profile of resveratrol from the nanocapsules in the in vitro simulated gastrointestinal tract was greatly enhanced and could be well fitted using the Higuchi kinetic model and Korsmeyer–Peppas model, indicating a Fickian diffusion release mechanism. Compared to native resveratrol, the bioaccessibility of resveratrol embedded in nanocomplexes and nanoparticles was increased to 60% and 80%, respectively. These findings suggest that OVA/CMC nanocomplexes and nanoparticles have potential applications in the development of effective oral delivery systems of resveratrol as well as other lipophilic nutraceuticals into functional foods.
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危险品化学品经营许可证（带存储）