Cell Discovery
·
2025
·Vol. 11
·期 1
·1-14
·
IF 12.5
Wang Jinrui,Niu Shenghui,Hu Xiao,Li Tianxing,Liu Shengduo,Tu Yingfeng,Shang Zehua,Zhao Lin,Xu Pinglong,Lin Jingwen,Chen Lu,Billadeau Daniel D.,Jia Da
The cGAS-STING pathway mediates the innate immune response to cytosolic DNA, contributing to surveillance against microbial invasion or cellular damage. Once activated, STING recruits TBK1 at the trans-Golgi network (TGN), which in turn phosphorylates IRF3 to induce type I interferon (IFN-I) expression. In contrast to STING, little is known about how TBK1 is transported to the TGN for activation. Here, we show that multiple TGN tethering factors, a group of proteins involved in vesicle capturing, are indispensable for STING-IFN-I signaling. Deletion of TBC1D23, a recently reported tethering factor, in mice impairs the STING-IFN-I signaling, but with insignificant effect on STING-NF-κB signaling. Mechanistically, TBC1D23 interacts with TBK1 via the WASH complex subunit FAM21 and promotes its endosome-to-TGN translocation. Furthermore, multiple TGN tethering factors were reduced in aged mice and senescent fibroblasts. In summary, our study uncovers that TGN tethering factors are key regulators of the STING-IFN-I signaling and suggests that their reduction in senescence may produce aberrant STING signaling.
研究领域:
免疫学
·
细胞生物学
·
分子生物学
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2024
·Vol. 28
·期 8
·2669-2677
·
IF 2.6
Xia Xiaohong,Duolihong Bawurejiang,Ma Xiangdong,Yue Shan,Liu Rui
Electrochemical biosensor based on horseradish peroxidase (HRP) modified gold nanoparticle/sulfur-doped graphene (AuNP/SG) has been developed for the quantification of trans -resveratrol (Res). Gold nanoparticle (AuNP) was selected as the carrier for HRP due to its excellent electrochemical catalytic activity, biocompatibility, and conductivity, while sulfur-doped graphene (SG) was selected as the support for AuNP due to the defects introduced in the carbon network by sulfur doping can facilitate the high dispersion state of AuNP and HRP. The results show that the excellent sensing performance of the HRP/AuNP/SG is attributed to the high charge polarization of material after sulfur dropping and the co-catalysis of HRP and AuNP. For the detection of Res, linear response can be observed with high sensitivity and low detection limit (4 × 10 −8 M). Further, this electrochemical biosensor presents strong reproducibility, stability, and anti-interference capability in detecting Res. These unique properties make it an excellent choice for Res reliable detection.
研究领域:
生物化学
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材料科学
·
分析化学
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APPLIED CATALYSIS A-GENERAL
·
2020
·
IF 5.006
Di Zhang,Jianjun Zhang,Dalin Sun
Low global warming potential (GWP) refrigerant cis-1,3,3,3-tetrafluoropropene (HFO-1234ze(Z)) is a promising alternative working fluid for high-temperature heat pump designed for heat recovery in the industrial sector. As part of an effort to mass production of HFO-1234ze(Z), the isomerization of trans-1,3,3,3-tetrafluoropropene (HFO-1234ze(E)) to HFO-1234ze(Z) is performed to develop a process with high selectivity and high yield. Herein, we prepared the alumina catalysts via calcination, fluorination, and alkaline modification and tested for the trans-cis isomerization of HFO-1234ze. As a result, the alumina catalyst with appropriate weak Lewis acid sites tended to form HFO-1234ze(Z) with selectivity levels of about 97 %–99 % and no deactivation was observed following time on stream of 200 h. Proposed reaction mechanisms were described to understand the reactions over alumina surface. The present work provides a highly efficient synthetic process for industrial production of HFO-1234ze(Z).
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Journal of Physical Chemistry C
·
2022
·
IF 4.177
Jun Han,Qunfei Zheng,Cheng Jin,Shanmin Wang,Ying Liu,Yusheng Zhao,Jinlong Zhu
The comparison of different stereoisomeric organic compounds under high pressure has been less investigated. Here, we chose different stereochemical configurations of cis/trans-stilbene to study the luminescence properties, polymerization reaction, and structural changes at 0–20 GPa by spectroscopy and XRD. No fluorescence enhancement occurred in cis-stilbene due to π–π stacking. At 16 GPa, the IR, UV–vis, and sample color changes show that it undergoes an irreversible polymerization, that C(sp2)–H changes to C(sp2 + sp3)–H. However, trans-stilbene undergoes fluorescence enhancement at 0–4 GPa due to the reduction of the torsion angle of the benzene ring and the C═C bond leading to the formation of rigid planar molecules, which is further confirmed by the IR and XRD results. At 8 GPa, the new peaks in UV–vis and XRD results show the formation of new substances by structural change. However, the structure of trans-stilbene is more stable, which leads to the return to the raw state after releasing the pressure, and a reversible transformation occurs at high pressure. The cis-trans isomerization under high pressure was also briefly investigated by combining heating and laser irradiation. The cis → trans-stilbene transition can only happen under a fixed-range light irradiation, and the trans → cis-stilbene transition could not happen even under irradiation with a 360 nm laser, which may provide a new idea for synthesizing trans isomers with a higher purity.
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Catalysts
·
2023
·Vol. 13
·期 5
·809
·
IF 3.9
Elrasheed Elhaj,Huajun Wang,Enaam A. Al-Harthi,Waseem A. Wani,Sahar Sallam,Nasser Zouli,Mohd Imran
In this work we demonstrate one-pot glycidol synthesis, via trans-esterification between glycerol and dimethyl carbonate, by making use of commercially available sodium methoxide as a catalyst. An excellent glycerol conversion (99%) and remarkable glycidol yield (75%) was obtained using dimethyl carbonate/glycerol (molar ratio 2:1) in the presence of 3 wt% catalyst amount (with respect to glycerol weight) at 85 °C for a reaction time of 120 min. Sodium methoxide was recycled and reused twice with only a slight decrease in glycerol conversion. The water content of the glycerol reached 2.5 wt%; this did not reduce the glycerol conversion efficiency of the catalyst. A plausible mechanism for the trans-esterification involved in the preparation of glycidol was proposed.Keywords:glycerol carbonate;one-pot glycidol synthesis;sodium methoxide-catalysed;stability of sodium methoxide catalyst;trans-esterification of glycerol
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Zihan Xu,Shanshan Liu,Mingyue Shen,Jianhua Xie,Jun Yang
Trans fatty acids (TFAs), associated with the risks of coronary heart disease and diabetes, are formed by isomerization of unsaturated fatty acids during refining of linseed oils. In this study, TFAs and the chemical characteristics (acid value, peroxide value, carbonyl compounds, bioactive minor components and fatty acids) in 32 commercial linseed oils were investigated, and the correlation among them were further analyzed. Results showed that C18:3 TFAs were predominant TFAs in linseed oils and about 9% of the samples had TFA contents above 2 g/100 g fat, as well as the average level of TFA in the refined samples was higher than that in the unrefined oils. The correlation analyses suggested C18:3 TFAs exhibited significant negative correlations with acid value, levels of acetone, trans -2-nonenal, campesterol and α-linolenic acid. These results provided a comprehensive insight of TFAs in linseed oil and had important implications for consumers and linseed oil industry.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2019
·Vol. 31
·期 1
·107-113
·
IF 2.162
Lin Xia,Hao Wu,Guixue Qiu
Shape memory composites of trans -1,4-polyisoprene (TPI) and low-density polyethylene (LDPE) with easily achievable transition temperatures were prepared by a simple physical blending method. Carbon nanotubes (CNTs) were introduced to improve the mechanical properties of the TPI/LDPE composites. The mechanical, cure, thermal, and shape memory properties of the TPI/LDPE/CNTs composites were investigated in this study. In these composites, the cross-linked network generated in both the TPI and LDPE portions acted as a fixed domain, while the crystalline regions of the TPI and LDPE portions acted as a domain of reversible shape memory behavior. We found that CNTs acted as not only reinforced fillers but also nucleation agents, which improved the crystalline degree of the TPI and LDPE portions of the composites. Compared with the properties at the other CNT doses, the mechanical properties of the TPI/LDPE composites when the CNT dose was 1 phr were improved significantly, showing excellent shape memory properties ( R f = 97.85%, R r = 95.70%).
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ULTRASONICS SONOCHEMISTRY
·
2019
·
IF 7.279
Lijun Geng,Xudong Yu,Yanqiu Wang,Yajuan Li,Fengjuan Shen,Jujie Ren
Herein, instant and precise control of fluorescent emission color and transmittance could be carried out by ultrasound-promoted gel-to-gel transition of naphthalimide derivatives containing C N unit. It is proved that ultrasound triggered an irreversible and efficient configuration transformation of N1 from “trans to cis” form in gel state, which is stabilized by intermolecular hydrogen bonding interaction and not observed in the solution state.
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INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
·
2019
·Vol. 60
·期 8
·3034-3045
·
IF 3.812
Yi Liao,Houjian Zhang,Danxue He,Yan Wang,Binxiang Cai,Jingmeng Chen,Jianxing Ma,Zuguo Liu,Yalin Wu
Purpose:Visual (retinoid) cycle anomalies induce aberrant build-up of all-transretinal (atRAL) in the retinal pigment epithelium (RPE), which is a cause of RPE atrophy in Stargardt disease type 1 and age-related macular degeneration. NLR family pyrin domain containing 3 (NLRP3) inflammasome activation is implicated in the etiology of age-related macular degeneration. Here, we elucidated the relationship between NLRP3 inflammasome activation and atRAL-induced death of RPE cells.Methods:Cellular toxicities were assessed by MTS or MTT assays. Expression levels of mRNAs and proteins were determined by quantitative reverse transcription–polymerase chain reaction, Western blotting, or enzyme-linked immunosorbent assay. Fluorescence microscopy was used to examine intracellular signals. Ultrastructural features of organelles were examined by transmission electron microscope.Results:Abnormal accumulation of atRAL was associated with a significant increase in the proportion of human ARPE-19 cells exhibiting features of apoptosis and Caspase-3/gasdermin E (GSDME)-mediated pyroptosis. These cells also exhibited elevated expression of NLRP3, ASC, cleaved Caspase-1/poly ADP-ribose polymerase (PARP)/Caspase-3/GSDME, interleukin-1β (IL-1β), and IL-18, as well as NLRP3 inflammasome-related genes (IL1BandIL18). After exposure of human ARPE-19 cells to excess atRAL, reactive oxygen species (ROS) (including mitochondrial ROS) and cathepsins released from lysosomes transmitted signals leading to NLRP3 inflammasome activation. Suppressing the production of ROS, NLRP3 inflammasome, Caspase-1, cathepsin B, or cathepsin D protected ARPE-19 cells against atRAL-associated cytotoxicity. Damage to mitochondria, lysosomes, and endoplasmic reticulum in atRAL-exposed ARPE-19 cells was partially alleviated by treatment with MCC950, a selective NLRP3 inflammasome inhibitor.Conclusions:Aberrant build-up of atRAL promotes the death of RPE cells via NLRP3 inflammasome activation.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Yanbin Su,Xiaowei Ma,Ning Jiang,Qingsong Zhang,Mengjie Li,Yuan Li,Shuxin Li
The purposes of this study were to probe spectral behaviors of the toxic targets in trans-crotonaldehyde (TCA) in mitochondria altered by diallyl disulfides (DADS) derived from garlic. Ultraviolet absorption spectra showed that when ethanol as a solvent, the DADS blue shifted the peak of TCA from 318 nm to 312 nm. In mitochondria, the DADS further blue displaced the peak of TCA from 312 nm to 308 nm. Raman spectra displayed that the S S of DADS directly interacted with the C C toxic target of TCA, then the C C C of DADS interacted with the CH O toxic target of TCA. When DADS to TCA was 1:2, the DADS was the most powerful for the removal of the C C and CH O toxic targets of TCA. Study suggested that the S S of DADS altered the C C toxic target of TCA, while the C C C of DADS eliminated the CH O toxic target of TCA via local electron delocalization . The above two together clearly depicted the spectral behaviors of the toxic targets of TCA in mitochondria altered by DADS. These results are of great significance and value to elucidate the effects of garlic organic polysulfide on myocardial ischemia for the extensive development and use of garlic extracts.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2015
·Vol. 34
·期 1
·104-110
·
IF 1.835
Liu Bo,Liu Dong-tao,Li Shi-hui,Sun Guang-ping,Cui Dong-mei
β -Iminophosphonamide ligated lanthanum bis(benzyl) complex (NPN Dipp )La(CH 2 Ph-4-Me) 2 (THF) (NPN Dipp = Ph 2 P(NC 6 H 3
i Pr 2 -2,6) 2 ), upon activation of AlEt 3 and [Ph 3 C][B(C 6 F 5 ) 4 ], exhibited high catalytic activity and high trans -1,4 stereoselectivity for the polymerization of bio-sourced β -myrcene (MY). Based on which, a series of novel trans -1,4 regulated elastomers could be generated by random/block copolymerization of MY and isoprene (IP).
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2016
·
IF 1.835
Juan Li,Zuo-Xiang Zeng,Li Sun,Wei-Lan Xue,Hui-Hui Wang
The solubilities of trans-cinnamic acid (t-CA) in methanol, ethanol, propanol, n-butanol, isopropyl alcohol, and isobutyl alcohol were measured by a synthetic method at temperatures ranging from (283.15 to 333.15) K. The modified Apelblat equation, the λh equation, and activity coefficient models (nonrandom two-liquid, NRTL; universal quasichemical, UNIQUAC) were used to correlate these data. It was found that all of the models show good agreement with the experimental data, and the modified Apelblat equation and NRTL model give better correlation results. Furthermore, on the basis of the NRTL model and experimental data, the thermodynamic excess functions (GE, SE, HE) of t-CA + alcohols (methanol, ethanol, propanol, n-butanol, isopropyl alcohol, and isobutyl alcohol) system were determined.
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