查找使用阿拉丁产品的科研文献

Functional Magnetic Nanoparticles for Clinical Application: Electrochemical Immunoassay of Hepatitis B Surface Antigen and α-Fetoprotein

Qiong Cheng,Jun-Feng Li,Liqiu Zhang,Lichun Liu

This work reports an efficient method to quantify the Hepatitis B surface antigen and α-fetoprotein in human serum using a functional magnetic nanoparticle-assisted sandwich-type electrochemical immunoassay. The Fe 3 O 4 magnetic nanoparticles were first modified with carboxyl functional groups to permit stable bioconjugation to the amine groups of most biological targets. The primary antibodies were then covalently stained on the surface of the functional magnetic nanoparticles, followed by the analyte and secondary antibodies, resulting in a sandwich-type (antibody-antigen-antibody/enzyme) immune complex. The secondary antibodies were labeled with horseradish peroxidase for the catalytic oxidation of 2-aminophenol to yield electrochemically reducible molecules. The separation using an external magnetic field guaranteed fast and reliable purification and enrichment of analytes. Quantitative analysis was performed upon representative clinical targets: Hepatitis B surface antigen and α-fetoprotein in human serum. The detection limits were 0.06 ng/mL for the former and 0.5 ng/mL for the latter, which were about 10 times lower than values obtained by conventional enzyme-linked immunosorbent assays. The reported method may be adopted as a general strategy for the sensitive and selective determination of additional proteins and biological molecules.

Band alignment and enhanced photocatalytic activation for α-Bi2O3/BiOCl (0 0 1) core–shell heterojunction

Lian-wei Shan,Gui-lin Wang,Li-zhu Liu,Ze Wu

In order to develop low-cost photocatalyst, commercial Bi 2 O 3 was utilized as raw material to prepare α-Bi 2 O 3 /BiOCl core-shell heterojunction via a facile in situ chemical transformation method. To clarify experimentally the band alignment relationships between the α-Bi 2 O 3 and BiOCl (0   0   1), the α-Bi 2 O 3 /BiOCl (0   0   1) core–shell heterojunction band offsets were analyzed by X-ray photoelectron spectroscopy (XPS). Here based on energy gaps, valence band values of α-Bi 2 O 3 and BiOCl (0   0   1), core levels difference of Bi 4f in α-Bi 2 O 3 /BiOCl (0   0   1) heterojunction , we demonstrate that band alignment of ∼0.28   eV exists between α-Bi 2 O 3 and BiOCl with α-Bi 2 O 3 possessing the higher valence band potential or electron affinity which is a driving force of valence band holes from α-Bi 2 O 3 to BiOCl (0   0   1). While, the electrons in conduction band were driven to migrate through conduction band potential difference (0.49   eV) from BiOCl (0   0   1) to α-Bi 2 O 3 .

Synthesis of uniform two-dimensional non-layered α-MnSe by molecular sieves modified chemical vapor deposition

Meijie Zhu,Hua Xu,Zuoquan Tan,Le Wang

Large-area and uniform two-dimensional (2D) non-layered α-MnSe was successfully synthesized via the molecular sieve modified chemical vapor deposition (CVD). The molecular sieve serves both as a filter and a container to controllable release the Mn precursor in a stable and much lower concentration, leading to the uniform growth of 2D α-MnSe flakes. The thickness, crystalline quality and uniformity of the as-grown α-MnSe are further characterized. Our study validated the positive role of molecular sieve in the controllable growth of 2D α-MnSe, which is believed to be applicable to other non-layered transition metal chalcogenides (TMCs) and will be beneficial for their further applications in electronics, optoelectronics and spintronics.

A novel low-temperature approach for fabricating α-Al2O3-based ceramic coating as tritium permeation barrier

Heping Li,Zhiqiang Ke,Lihong Xue,Youwei Yan

α-Al 2 O 3 -based coatings have attracted great interest as promising tritium permeation barrier used in the D-T fusion reactor system. However, it is still difficult to fabricate α-Al 2 O 3 coatings at a low temperature that can be endured by the steel substrate. Herein, thermal-chemical reaction method was developed for the fabrication of α-Al 2 O 3 -based coatings. α-Al 2 O 3 /AlPO 4 composite coating was successfully formed on the steel substrate at 500   °C. The resulted coating was uniform and compact without presence of pores and cracks. The binding strength of the coating was tested and the result indicated its good adherence to the substrate. Moreover, the coating thickness could be easily controlled. The low-temperature approach developed is expected to provide a new platform for fabricating α-Al 2 O 3 -based coatings as tritium permeation barrier. This approach is also applicable to the fabrication of other ceramic coatings.

Copper-Catalyzed Three-Component Difunctionalization of Aromatic Alkenes with 2-Amino-1,4-naphthoquinones and α-Bromocarboxylates

Yu Shangguan,Fazhou Yang,Hao Deng,Hao Liu,Ziyan Liu,Wanyue Zhuang,Chenxi Qiao,Aizheng Wang,Yumei Xiao,Cheng Zhang

A copper-catalyzed three-component difunctionalization of aromatic alkenes to access 1,4-naphthoquinone derivatives with diverse structures is described. Experiments show that the difunctionalization reaction is accompanied by ester exchange reaction with the solvent. In this method, α-bromocarboxylates are used as radical precursors and 2-amino-1,4-naphthoquinones as radical trapping reagents. The substrate scope is broad because various aromatic alkenes, 2-amino-1,4-naphthoquinones, and α-bromocarboxylates are employed in the reaction, and corresponding products are obtained in moderate to good yields.

Comparative insights into the role of oxygen vacancies in α-MnO2 for activating peroxymonosulfate and peroxydisulfate

Qianwei Li,Daoqing Liu,Wei Yan,Hao Liu,Chunmao Chen

Oxygen vacancies (OVs) play a critical role in enhancing the catalytic performance of transition-metal oxides in advanced oxidation processes (AOPs). However, the effect of OVs on the activation of different persulfates, peroxymonosulfate (PMS) and peroxydisulfate (PDS), is still not well understood. Here, we employ ultrasonic defect engineering to adjust the OV content of α-MnO2 and systematically benchmark its activation of PMS and PDS. XPS, EPR, and electrochemical analyses revealed that the ultrasound-treated α-MnO2 exhibited a significantly increased proportion of Mn(III) and a markedly higher OV concentration, thereby creating densely populated redox-active sites for persulfate adsorption and electron transfer. Mechanistic investigations indicated that in the PMS system, the oxidation pathway was closely associated with OVs: at low OV densities, radical oxidation (SO4˙−/˙OH) predominates, whereas at high OV densities, a non-radical route dominated by singlet oxygen (1O2) prevails. In contrast, the oxidation pathway in the PDS system exhibited a weak correlation with OV content, implicating distinct adsorption/activation motifs for PMS versus PDS on α-MnO2. Radical scavenging experiments and EPR analyses provided supportive, semi-quantitative evidence for these mechanistic trends, within the acknowledged limitations of current ROS identification methods. Collectively, this work establishes a clear structure–reactivity correlation and provides a defect-engineering blueprint for tailoring targeted reactive oxygen species in water purification applications.

Correction: α-Glycerol monolaurate promotes tight junction proteins expression through PKC/MAPK/ATF-2 signaling pathway

Siyu Dai,Cunjie Li,Dagang Li,Hong Wang

Interstitial carbon doping synergistically boosts bulk charge transport and surface OER kinetics of α-Fe2O3 photoanodes

Wenjie Liu,Qinghua Sun,Chenyang Liu,Yixin Chen,Jie Zhang,Weiwei Xia

Photoelectrochemical (PEC) water splitting using α-Fe 2 O 3 photoanodes is severely limited by poor bulk charge transport and sluggish surface oxygen evolution reaction (OER) kinetics. Herein, we report a novel interstitial carbon doping strategy via glucose-assisted hydrothermal synthesis and annealing to synergistically address these bottlenecks. Unlike conventional carbon modifications (surface coating, which only improves interfacial properties, or substitutional doping, which induces lattice distortion), interstitial carbon doping simultaneously enhances bulk charge transport and surface OER activity without structural compromise. Specifically, interstitial C atoms induce lattice compression (confirmed by XRD peak shifts), increase carrier density to 2.57 × 10 18 cm -3 (6.2-fold higher than pristine α-Fe 2 O 3 ), and reduce electron transport time to 0.20 ms, while surface C-O/C=O groups accelerate OER kinetics by facilitating intermediate adsorption. The optimized C50-α-Fe 2 O 3 photoanode exhibits a photocurrent density of 1.80 mA/cm 2 at 1.23 V vs. RHE (6.3-fold enhancement), an onset potential negatively shifted to 0.82 V vs. RHE, and a maximum applied bias photon-to-current efficiency (ABPE) of 0.21 % at 1.02 V vs. RHE. This work demonstrates interstitial doping as a versatile approach to decouple bulk/surface property optimization, advancing α-Fe 2 O 3 for efficient solar-to-hydrogen (STH) conversion.

Tollens Reagent-Directed Flower-like α-Ag3VO4 Nanozyme for Signal-Amplified Colorimetric Detection of Epigallocatechin Gallate

Fu-Long Lu,Shu-Jie Feng,Yong-Fei Ji,Yun-Yun Wu,Xiu-Shuang Yang,Hao-Yu Ma,Wen-Sheng Zhang,Dong-Dong Qin,Wei Wang,Dong-Xue Han,Li Niu

Oxidase-like colorimetric sensors based on crystalline vanadates show great potential for polyphenol detection without H2O2. However, most inorganic nanozymes possess multienzyme activities and follow the “on-to-off” sensing mode, limiting sensitivity and accuracy in complex matrices. Herein, a flower-like α-Ag3VO4 nanozyme was synthesized via a Tollens reagent-mediated method. Distinct from conventional vanadate-based materials, it activates O2 molecules adsorbed on its oxygen-defective (010) surface through 0.86 eV energy transfer, thereby exerting exclusive oxidase-like activity with O2•– and 1O2 as electron acceptors. EGCG accelerates charge transfer and boosts catalytic activity by over 6-fold within 1 min, allowing for an “off-to-on” colorimetric sensor. This unique phenomenon enables the construction of a rapid colorimetric sensor based on an “off-to-on” signal model, which differs fundamentally from reported nanozyme-based colorimetric systems for reducing targets. Compared with the widely used aluminum chloride method (detection limit, LOD = 3.54 μM), the proposed sensor exhibits excellent specificity, superior sensitivity (LOD = 0.16 μM) for EGCG, and comparable accuracy in real sample analysis. This work not only provides a synthetic chemistry approach to tailor the enzyme-like activity of inorganic materials but also offers a promising platform for the sensitive detection of reducing polyphenols.

Icariin ameliorates sarcopenia via activation of the estrogen receptor α/fatty acid transport protein 1 pathway

Li Song,Yue Li,Wenjing Li,Bingfeng Lin,Wenlong Xiao,Yuzhen Xia,Feida Wang,Mingli Ye,Wei Wang,Nani Wang

Background Sarcopenia is a prevalent disorder among postmenopausal women, representing a debilitating condition with limited treatment options. Icariin (ICA), a prenylated flavonol glycoside, is commonly used in conditions associated with estrogen deficiency; however, its efficacy and mechanism in postmenopausal sarcopenia remain undefined. Purpose This study aimed to evaluate the therapeutic effect of ICA on postmenopausal sarcopenia and to elucidate its underlying mechanism, with a focus on the estrogen receptor α/fatty acid transport protein 1 (ERα/FATP1) pathway. Study design An integrated approach was employed, combining in vivo pharmacological evaluation, multi-omics analyses, and in vitro mechanistic validation. Methods The in vivo therapeutic effect of oral ICA was assessed by evaluating muscle function, histology, and senescence markers. Human muscle transcriptome datasets and mouse single-cell RNA sequencing data were analyzed. In vitro validation was performed in D-galactose-induced senescent C2C12 myoblasts. Target engagement was confirmed by molecular docking, cellular thermal shift assay, and surface plasmon resonance, and mechanistic validation was performed via FATP1 knockdown. Results Oral administration of ICA significantly improved muscle mass, function, and fiber cross-sectional area, while attenuating lipid deposition and cellular senescence. Single-cell RNA sequencing revealed a diminished myoblast pool with downregulated ERα in aged muscle. This finding aligns with human transcriptome data, linking reduced ERα/FATP1 signaling and dysregulated fatty acid metabolism to sarcopenia. Metabolomic analysis identified FATP1 as a critical transporter of eicosapentaenoic acid and docosapentaenoic acid, which activated the protein kinase B/ mammalian target of rapamycin pathway to promote myogenesis. Importantly, ICA functioned as a dual-target agonist, directly binding to and upregulating both ERα and FATP1, thereby elevating eicosapentaenoic/ docosapentaenoic acid levels and reactivating the protein kinase B/ mammalian target of rapamycin/ myogenic differentiation 1 axis. The specificity of this pathway was confirmed, as FATP1 knockdown completely abrogated the protective effects of ICA. Conclusion This study identifies the ERα/FATP1 axis as a pivotal therapeutic target for sarcopenia. ICA, acting as a first-in-class dual agonist of this pathway, represents a promising candidate for sarcopenia treatment by synchronously modulating estrogen signaling and fatty acid metabolism. Download: Download high-res image (181KB) Download: Download full-size image

Atorvastatin Shows Limited Disease-Modifying Effects in an A53T α-Synuclein Mouse Model of Parkinson’s Disease

Wei Lijian,Hua Junkai,Tang Shuangfeng,Lei Jianming,Li Guozhi,Wei LiWei,Mo MingShu

Neuroprotective effects of statins in Parkinson’s disease (PD) remain uncertain, and their activity in genetic α-synuclein (αSyn) disease models has been insufficiently characterized. We evaluated atorvastatin (ATO) in a mouse model with nigral overexpression of human A53T-mutant αSyn. Mice received ATO by oral gavage at 10 mg/kg/day for 5 weeks and were assessed using behavioral testing, neuropathological assessment, brain transcriptomics, and molecular docking. ATO inhibited cholesterol biosynthesis-related transcriptional programs and broadly remodeled lipid metabolism-associated networks, but did not lead to functional or histopathological benefit. ATO did not ameliorate motor deficits, restore dopaminergic markers, or reduce αSyn protein levels or pSer129-αSyn immunoreactivity. Transcriptomic analysis further showed that ATO failed to reverse the core disease-associated signature induced by A53T αSyn overexpression and instead increased SNCA mRNA. Targeted RNA-seq and western blot analyses showed no parallel increase in Prkn , Gba1 , or Lamp2 transcript abundance or in PARKIN, GBA1, or LAMP2A protein expression. Molecular docking, used here as an exploratory structural comparison, suggested a relatively weak predicted interaction between ATO and αSyn when compared with several other statins and provided supportive context for the lack of efficacy. Overall, our findings indicate limited efficacy of ATO in this αSyn-driven setting and support further comparative evaluation of individual statins across complementary PD models. Graphical

P-doping reinforces the crystal phase stability of α-MnO2 cathodes for aqueous zinc-ion batteries

Hanjie Si,Jun Ma

Phosphorus-doped α-manganese dioxide cathode material was successfully synthesized. • P-α-MnO 2 possesses excellent electrochemical performance. • P doping can enhance structural stability of the α-MnO 2 phase. MnO 2 is recognized as a highly competitive cathode candidate for aqueous zinc-ion batteries (AZIBs) thanks to its low fabrication cost and considerable reversible capacity. However, MnO 2 suffers from severe manganese dissolution and structural phase transitions during cycling, leading to crystalline phase instability and thus severely limiting further performance enhancement of AZIBs. Herein, we successfully construct Phosphorus‑incorporated α‑MnO 2 (P-α-MnO 2 ) via an ion doping strategy and evaluate its electrochemical performance in AZIBs. PMO-2 delivers a peak capacity of 440.7 mAh g −1 at 0.1 A g −1 after 20 cycles and sustains 89% capacity (192–170.8 mAh g −1 ) upon 1500 cycles at 1.5 A g −1 , exhibiting better retention compared with pristine α-MnO 2 that maintains merely 49.6% (159–78.9 mAh g −1 ). Combined structural characterizations and DFT calculations indicate that the boosted performance of P-α-MnO 2 stems from phosphorus doping strengthening the crystalline stability of α-MnO 2 . It can substantially cut down Zn 2+ adsorption energy as well as ion‑migration barriers, which alleviates lattice‑channel destruction caused by Zn 2+ insertion‑extraction and preserves the tunnel‑type crystal framework of α‑MnO 2 throughout charge‑discharge operations. This work provides important theoretical foundations and design strategies for the further development of low-cost, high-stability, and high-energy-density manganese-based cathode materials. Download: Download high-res image (148KB) Download: Download full-size image