JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 6.8
Yu Yang,Hongda Liu,Junling Ren,Lin Liu,Hui Sun,Li Wang,Guangli Yan,Ying Han,Xijun Wang
Ethnopharmacological relevance Danggui Buxue Decoction (DBD) is a classic traditional Chinese medicine formula that has shown significant efficacy in treating blood deficiency syndrome (BDS), though its mechanisms of action remain unclear. Aim of the study To identify the absorbed bioactive components of DBD and elucidate its molecular mechanisms in BDS by integrating metabolomics and bioinformatics. Materials and methods Using a benzene induced BDS mouse model, we analyzed serum-absorbed components of DBD via UPLC-Q/TOF-MS. A multi-omics approach combining metabolomics, molecular docking, and dynamics simulations was utilized to identify therapeutic targets and bioactive components, followed by experimental validation using pharmacological assays, Western blot, and cellular thermal shift assay (CETSA). Results In the BDS mouse model, DBD primarily corrected nicotinate and nicotinamide metabolism and improved hematopoietic function. Furthermore, it significantly decreased the levels of inflammatory cytokines that activate the STAT3 signaling pathway, including interleukin-6 (IL-6), interleukin-17 (IL-17), and transforming growth factor-β (TGF-β). It also suppressed the expression of p-JAK2/JAK2 and p-STAT3/STAT3. Under the effective status of DBD, we identified eleven prototype components and three metabolites derived from DBD, among which astragaloside IV was a high-affinity binder to STAT3, with molecular dynamics simulations and CETSA confirming its binding stability. Conclusion Our integrated approach suggests that DBD may alleviate BDS by normalizing metabolic imbalance and suppressing the IL-6/JAK2/STAT3 signaling pathway. A role for astragaloside IV as a potent bioactive compound of DBD with high-affinity binder to STAT3 is supported by molecular dynamics simulations and CETSA experiments. These findings provide a mechanistic foundation for DBD's therapeutic effects in treating BDS.
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高影响因子
BRITISH JOURNAL OF PHARMACOLOGY
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2026
·
IF 7.5
Honglian Li,Zhihao Ni,Yuzhen Rao,Junzhe Zhang,Huiyi Guo,Haoxing Yuan,Hao Yang,Jiayun Chen,Chan Yang,Jigang Wang,Yu Rao,Shuwen Liu
Background and Purpose Lupus nephritis (LN) is a common complication of systemic lupus erythematosus (SLE) that symbolizes a primary contributor of morbidity and mortality. Dihydroartemisinin (DHA; artenimol) shows good therapeutic effects on LN. Its pharmacological target remains unclear. Proteolysis targeting chimeras (PROTAC) technology was used to identify the targets of DHA in LN treatment. Experimental Approach The activity of a novel compound termed A1 was assessed through cytotoxicity and anti-inflammatory assays in vitro . The target of DHA was screened by the A1 integrating with proteomics and then validated via western blotting, co-immunoprecipitation (Co-IP), immunofluorescence, molecular docking, cellular thermal shift assay (CETSA) and drug affinity responsive target stability (DARTS) in vitro. Twenty-four-hour proteinuria, inflammatory cytokines, serum biochemical indicators, immune complex deposition and renal pathological injury were detected. Adeno-associated virus-mediated RNA interference in silencing CARD6 was conducted in vivo to comprehensively evaluate the effect and the target of DHA on MRL/lpr mice. Key Results CARD6 was the most notably degraded protein affected by A1 that identified as the target of DHA. Specifically, DHA interacted with CORE-CARD6 at the 481–949aa region. DHA blocked CARD6 to inhibit RIP2/NF-κB pathway, which manifested anti-inflammatory properties in vivo and in vitro . Silencing CARD6 ameliorated the inflammatory response of MRL/lpr mice. Notably, the inhibition effects of DHA on the MRL/lpr mice were abrogated when CARD6 was depleted. Conclusion and Implications CARD6 was a therapeutic target of DHA for LN management. This finding highlights the great potential of using PROTAC technology to effectively identify therapeutic targets of natural products. Graphical CARD6 was the most notably degraded protein affected by the novel compound PROTAC A1 that was identified as the target of dihydroartemisinin (artenimol; DHA) for lupus nephritis (LN) treatment. DHA demonstrated anti-inflammatory properties in vitro and renal protective effect in vivo by targeting CARD6/RIP2/NF-κB signalling pathway.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Liyuan Zhang,Qiumeng Zheng,Xinchang Ge,Hongyu Chan,Guoqing Zhang,Kuanjun Fang,Yueyao Liang
Compared of traditional melt-blown nonwoven materials, micro-nanofiber composite membranes with uniform spatial gradient structure will be the development trend of high efficiency and low resistance filtration materials, especially against ultrafine particles with kinetic diameter less than 0.25 (PM 0.25 ). Herein, Nylon-6 micro-nanofiber composite membranes (Nylon-6 FCMs) with three-dimensional (3D) uniform gradient structure were prepared by air jet spinning under the help of PEO. The fluffy 3D gradient structure possessed a uniform gradual pore gradient from large to small, ensuring the PM 0.25 were captured by exact grading under high gas flow due to the form of special “trumpet-like” gas passage inside the membranes. The structure of Nylon-6 FCMs could be controlled and exhibited high tensile strength, good moisture permeability, excellent filtration performance. Among them, the FCM-1 with a uniform gradual pore gradient could achieve the optimal filtering performance with filtration efficiency (99.99 %) and pressure drop (144 Pa). The mask prepared using this Nylon-6 FCMs also displayed good protective effect with comparable air permeability (221.84 mm·s −1 ) and moisture permeability (181.84 g·m −2 ·h −1 ) compared of commercial melt-blown masks. Most importantly, this mask prepared still could maintain good filtration performance even in high temperature and high humidity environment, providing users more comfortable wearing experience and stable protection performance, especially under the current COVID-19 outbreak.
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Journal of Hard Tissue Biology
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2022
·
IF 0.593
Jie Zhang,Yongli Wang,Jianzhong Zhang,Shaoyan Huang
We investigated the anti-inflammatory effect of hydroxyethyl starch (HES) 6% 200/0.5 on lipopolysaccharide (LPS)-induced RAW264.7 cells based on the high mobility group protein B1/nuclear transcription factor-κB (HMGB1/NF-κB) signaling pathway. The mouse monocyte macrophage RAW264.7 cells were divided into control group (no additional treatment), LPS group (0.2 pg/ml LPS), LPS+HES group (0.2 pg/ml LPS+6% HES) and LPS+TAK-242 (HMGB1/NF-κB signal pathway blocker) group (0.2 pg/ml LPS The LPS+HES+TAK-242 group (0.2 pg/ml LPS+6% HES+1 μ mol/l TAK-242) was cultured for 48 hours. Griess method and ELISA were used to detect the levels of nitric oxide (NO), tumor necrosis factor α (TNF-α), interleukin (IL)-6 and IL-1β in cell supernatant. The level of intracellular reactive oxygen species (ROS) was detected by flow cytometry. The mitochondrial membrane potential was detected by immunofluorescence. The mRNA expressions of inducible nitric oxide synthase (iNOS), HMGB1, Toll-like receptor 4 (TLR4), nuclear factor-κ b inhibitory protein α (IκBα) and NF-κB were detected by qRT-PCR. The expressions of HMGB1, TLR4, IκBα, phosphorylated IκBα (p-IκBα), NF-κB and phosphorylated NF-κB (p-NF-κB) were detected by western blotting. Compared with the control group, the expression levels of NO, TNF-α, IL-6, IL-1β, ROS, iNOS, HMGB1, TLR4, NF-κB mRNA, HMGB1, TLR4 and p-NF-κB proteins in the LPS group, LPS+HES group, LPS+TAK-242 group and LPS+HES+TAK-242 group increased (P<0.05), the expression levels of mitochondrial membrane potential, IκBα mRNA and p-IκBα protein decreased (P<0.05). Compared with LPS group, the expression levels of NO, TNF-α, IL-6, IL-1β, ROS, iNOS, HMGB1, TLR4, NF-κB mRNA, HMGB1, TLR4 and p-NF-κB proteins in the LPS+HES group, LPS+TAK-242 group and LPS+HES+TAK-242 group increased (P<0.05), the expression levels of mitochondrial membrane potential, IκBα mRNA and p-IκBα protein decreased (P<0.05). The expression levels of mitochondrial membrane potential, IκBα mRNA and p-IκBα protein increased (P<0.05). Six % HES 200/0.5 could down-regulate the expression of molecules related to HMGB1/NF-кB signaling pathway, and reduce the inflammatory response of RAW264.7 cells induced by LPS.
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Science China-Materials
·
2023
·Vol. 66
·期 5
·1779-1792
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IF 8.1
Yang Jia,Wang Mengxiao,Chen Tao,Yu Xiang,Qin Gang,Fang Xiaohan,Su Xiaoxiang,Chen Qiang
Supercapacitors exhibiting toughness, self-healing, and high specific capacitance have practical significance for use in flexible and wearable electronic equipment. To meet these requirements, a novel multifunctional gel polymer electrolyte (GPE) consisting of polyvinyl alcohol (PVA)-sodium alginate (SA)-K 3 [Fe(CN)] 6 -Na 2 SO 4 was prepared. In this GPE, K 3 [Fe(CN)] 6 plays three crucial roles by serving as a carrier donor, ionic crosslinking agent and redox-active mediator. Consequently, the usual conflict between the conductivity and mechanical properties of GPEs is alleviated to some extent. In addition, the electrode specific capacitance and the energy density of the assembled supercapacitor are obviously improved because of the pseudocapacitance generated from the redox reaction of K 3 [Fe(CN)] 6 . Based on this GPE, the supercapacitor exhibits outstanding bending and stretching stabilities, significant self-healing, and anti-freezing properties. Therefore, the prepared GPE and supercapacitor are promising for application in flexible and wearable electronic equipment with complex service conditions.
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FOOD CHEMISTRY
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2022
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IF 9.231
Yingzi Xiong,Yanan Cao,Lei Luo,Pao Li,Maiquan Li,Ruozhong Wang,Langtao Xiao,Xia Liu
An adsorbent-magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) were synthesized for the extraction of 6-benzylaminopurine (6-BA) using Fe 3 O 4 as magnetic core. The MIPs were prepared with methacrylic acid and sodium p-styrene sulfonate as bifunctional monomers. The adsorbents were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffractometer, thermogravimetric analysis and vibrating sample magnetometer. The adsorption properties were evaluated by static, kinetic and selective adsorption experiments. The MMIPs NPs exhibit a high adsorption capacity (37.63 mg g −1 ) and favorable imprinting factor (2.88) toward 6-BA. The chromatogram of 6-BA extraction using the MMIPs NPs as the adsorbent demonstrates that the matrix interference has been minimized. More importantly, MMIPs NPs can be applied to extracting 6-BA from mung bean sprout and cucumber with satisfactory recoveries (91.14–104.52%), and can be reused for at least five times. This work provides a new strategy to efficiently extract 6-BA from vegetables.
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Asian Journal of Organic Chemistry
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2022
·Vol. 11
·期 8
·e202200255
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IF 3.116
Zhiwei Chen,Xiaoxiao Huang,Jie Sun,Yanmin Liu,Ziwei Li
Graphical A facile and effective metal-free decarboxylative cyclization of N -methacryloyl-2-phenylbenzimidazole with arylthio (oxy) difluoroacetic acids or α , α -aryldifluoroacetic acids was proposed to prepare a series of aryldifluoromethylated benzimidazole[2,1- a ] iso -quinoline-6(5 H )-one derivatives in medium to good yields with good functional group tolerance, readily available raw materials and simple operation. In addition, experimental studies showed that the CF 2 group of arylthiodifluoroacetic acids plays a crucial role in this conversion. A facile and effective metal-free decarboxylative cyclization of N -methacryloyl-2-phenylbenzimidazole with arylthio (oxy) difluoroacetic acids or α , α -aryldifluoroacetic acids was proposed to prepare a series of aryldifluoromethylated benzimidazole[2,1- a ] iso -quinoline-6(5 H )-one derivatives in medium to good yields with good functional group tolerance, readily available raw materials and simple operation. In addition, experimental studies showed that the CF 2 group of arylthiodifluoroacetic acids plays a crucial role in this conversion.
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DRUG DELIVERY
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2021
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IF 6.419
Jianbo Li,Tiange Feng,Weijing Yang,Yaru Xu,Shuaishuai Wang,Huijie Cai,Zhilei Liu,Hong Qiang,Jinjie Zhang
Although Fraxinellone (Frax) isolated from Dictamnus albus L. possessed excellent anti-hepatic fibrosis activity, oral administration of Frax suffered from the inefficient therapeutic outcome in vivo due to negligible oral absorption. At present, the oral formulation of Frax is rarely exploited. For rational formulation design, we evaluated preabsorption risks of Frax and found that Frax was rather stable while poorly dissolved in the gastrointestinal tract (78.88 μg/mL), which predominantly limited its oral absorption. Further solubility test revealed the outstanding capacity of cyclodextrin derivatives (CDs) to solubilize Frax (6.8–12.8 mg/mL). This led us to study the inclusion complexes of Frax with a series of CDs and holistically explore their drug delivery performance. Characterization techniques involving 1H-NMR, FT-IR, DSC, PXRD, and molecular docking confirmed the most stable binding interactions when Frax complexed with 6-O-α-D-maltosyl-β-cyclodextrin (G2-β-CD-Frax). Notably, G2-β-CD-Frax exhibited the highest solubilizing capacity, fast dissolution rate, and superior Caco-2 cell internalization with no obvious toxicity. Pharmacokinetic studies demonstrated markedly higher oral bioavailability of G2-β-CD-Frax (5.8-fold that of free drug) than other Frax-CDs. Further, long-term administration of G2-β-CD-Frax (5 mg/kg) efficiently inhibited CCl4-induced hepatic fibrosis in the mouse without inducing any toxicity. Our results will inspire the continued advancement of optimal oral Frax formulations for anti-fibrotic therapy.
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JOURNAL OF IRON AND STEEL RESEARCH INTERNATIONAL
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2021
·Vol. 29
·期 7
·1063-1072
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IF 1.263
Sun Qian,Wang En-hui,Hou Xin-mei,He Zhi-jun,Liang Tong-xiang
Calcium aluminate cement (CAC) is widely used as a binder for refractory materials, and thus the improvement in compressive strength is of vital importance for CAC applied at high temperature. For this purpose, nano-Ca 10 (PO 4 ) 6 (OH) 2 additive with a ratio of 0.5–1.5 mass% was added with the water-cement ratio to be 0.4. X-ray diffraction and isothermal calorimetry analysis demonstrate that nano-Ca 10 (PO 4 ) 6 (OH) 2 additive can shorten the hydration process and promote the formation of main hydrates of CaAl 2 O 4 ·10H 2 O (CAH 10 ) and Ca 2 Al 2 O 5 ·8H 2 O (C 2 AH 8 ). In addition, scanning electron microscopy results suggest that nano-Ca 10 (PO 4 ) 6 (OH) 2 can protect CAH 10 and C 2 AH 8 from being destroyed during the calcination, guaranteeing that these thin lamellar crystals are intertwined to form the denser microstructure. Benefited from above effects, nano-Ca 10 (PO 4 ) 6 (OH) 2 can obviously improve the compressive strength of the CAC mortar samples cured for 7 d after calcination at 1100 °C, while the improving effect is dependent upon its contents. Especially, compared with the one without the additive, the compressive strength of the sample with 1.0% nano-Ca 10 (PO 4 ) 6 (OH) 2 is increased by 14%.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2021
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IF 5.302
Bing Liu,Ke Sha,Meng Fei Zhou,Kai Xin Song,Yu Hui Huang,Cheng Chao Hu
Two novel low- ε r gallates MGa 2 O 4 (M = Ca, Sr) have been synthesized via a standard solid-state reaction method. According to the X-ray diffraction results, CaGa 2 O 4 crystallizes in space group Pna2 1 with an orthorhombic symmetry, while SrGa 2 O 4 belongs to the monoclinic P2 1 / c system. Both ceramics show ever-improving microstructures with the increasing sintering temperature . The optimal microwave dielectric properties ( ε r = 9.2, Qf = 66,000 GHz, τ f =–85 ppm/°C for SrGa 2 O 4 and ε r = 10.6, Qf = 15,400 GHz, τ f =–58 ppm/°C for CaGa 2 O 4 ) are obtained when sintered at 1275 °C. A patch antenna is fabricated using SrGa 2 O 4 ceramics as the substrate, which realizes a return loss of –19.94 dB and total efficiency of –1.38 dB (72.8 % in power ratio) at 4.84 GHz. The exceptional performances indicate that SrGa 2 O 4 ceramics are promising candidates for antenna applications at the Sub-6 GHz band.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2020
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IF 5.774
Rehan Khan,Ihtisham Ul Haq,Heng Mao,Ao-Shuai Zhang,Li-Hao Xu,Hong-Gang Zhen,Zhi-Ping Zhao
Phenol is highly toxic and carcinogenic even at low concentrations. This study focused on preparing high-performance mixed matrix membranes (MMMs) for the pervaporation separation of phenol from its aqueous solution. A highly-efficient zeolitic metal azolate framework, RHO-[Zn(Heim) 2 ] (MAF-6, Heim = 2-ethylimidazole) was incorporated into the polyether block amide (PEBA-2533) supported on the polyvinylidene fluoride (PVDF) ultrafiltration membrane. The MAF-6 was chosen as the filler due to its inherent hydrophobic surfaces and aperture size (7.6 Å), which is larger than the molecular kinetic diameter of phenol (6.6 Å). Effects of the casting solution concentration, active layer thickness, MAF-6 loading, operating temperature, and phenol concentration were systematically investigated. With the increase of the MAF-6 loading ratio X from 0 to 7, the phenol flux of the membrane was gradually enhanced, but the water flux was reduced. For 1000 ppm phenol aqueous solution at 80 °C, MAF-6–7@MMM displayed the optimal separation factor of 25.9 with a phenol flux of 89.2 g∙m −2 ∙h −1 . The pervaporation separation index (PSI) of the MAF-6–7@MMM was 1317.7 kg∙µm∙m −2 ∙h −1 at 80 °C, which was much higher than the PEBA/PVDF membrane (787 kg∙µm∙m −2 ∙h −1 ). Impressively, an increasing feed temperature enhanced the phenol flux more prominently than water, owing to the hydrophobicity and porosity of MAF-6. Consequently, the resultant MAF-6-X@MMMs broke the trade-off limitation between phenol permeability and selectivity. The as-prepared membrane also exhibited exceptional long-term stability, suggesting its prospect of industrial application.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
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IF 7.1
Yawen Wu,Xiaolong Chen,Xianfeng Wang,Mei Yang,Faliang Xu,Changjun Hou,Danqun Huo
The effective detection of CA15-3 is of great significance for the postoperative monitoring and early diagnosis of breast cancer. Here, a fluorescent biosensor based on the fluorescence resonance energy transfer (FRET) between the prismatic hollow Metal-polydopamine frameworks (PHMPF) and 6-carboxyfluorescein (FAM)-labeled protein aptamer (FAM-Apt) was established to detect CA15-3. Characterization of SEM, TEM, EDS, FT-IR, XRD and XPS confirmed that the synthesized PHMPF was a polyhedral prismatic hollow organometallic frame containing the elements of C, N, O, and Co. PHMPF can quench the fluorescent group modified on the aptamer efficiently. Under optimized conditions, the proposed fluorescent biosensor can detect CA15-3 low to 2.56 × 10 −5 U mL −1 . In addition, the excellent selectivity and spiked experiments indicate the proposed biosensor owns potential applications for the clinical diagnosis of tumor markers in body fluids.
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危险品化学品经营许可证（带存储）