RSC Advances
·
2023
·Vol. 13
·期 41
·29061-29069
·
IF 3.9
Xixi Wu,Huazhen Liang,Chaoming Li,Duanyang Zhou,Rui Liu
The specific tumor microenvironment is a conducive breeding ground for malignant tumors, favoring their survival, rapid proliferation, and metastasis, which is also an inevitable obstacle to tumor treatment, particularly for catalytic therapy. To address this issue, a hyperthermia-enhanced nanocatalyst (AuP@MnO2) consisting of an asymmetric Au@polypyrrole core and a MnO2 shell is constructed for synergistic cancer Fenton/photothermal therapy. In an ultra-short reaction time (15 min), the innovative introduction of a new oxidizer, tetrachloroauric acid trihydrate, not only successfully initiates the oxidative polymerization of pyrrole monomer while reducing itself to cubic Au, but also accelerates the polymerization process by supplying protic acid. After MnO2 coating, AuP@MnO2 catalyzes the conversion of antioxidant GSH and excess H2O2 into GSSG and ˙OH through Mn2+/Mn4+ ion couples, leading to oxidative damage of tumor cells. More importantly, after 1064 nm laser irradiation, more extreme oxidative imbalance and cell death are demonstrated in this work under the combined effect of photothermal and catalytic therapy, with insignificant toxicity to normal cells. This work develops an efficient one-step synthesis method of asymmetric Au@polypyrrole and provides constructive insight into its oxidative stress-based antitumor treatment.
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Scientific Reports
·
2016
·Vol. 6
·期 1
·1-7
·
IF 5.228
Guo Longhua,Xu Shaohua,Ma Xiaoming,Qiu Bin,Lin Zhenyu,Chen Guonan
Colorimetric enzyme-linked immunosorbent assay utilizing 3′-3-5′-5-tetramethylbenzidine(TMB) as the chromogenic substrate has been widely used in the hospital for the detection of all kinds of disease biomarkers. Herein, we demonstrate a strategy to change this single-color display into dual-color responses to improve the accuracy of visual inspection. Our investigation firstly reveals that oxidation state of 3′-3-5′-5-tetramethylbenzidine (TMB 2+ ) can quantitatively etch gold nanoparticles. Therefore, the incorporation of gold nanoparticles into a commercial TMB-based ELISA kit could generate dual-color responses: the solution color varied gradually from wine red (absorption peak located at ~530 nm) to colorless, and then from colorless to yellow (absorption peak located at ~450 nm) with the increase amount of targets. These dual-color responses effectively improved the sensitivity as well as the accuracy of visual inspection. For example, the proposed dual-color plasmonic ELISA is demonstrated for the detection of prostate-specific antigen (PSA) in human serum with a visual limit of detection (LOD) as low as 0.0093 ng/mL.
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MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Xiaoli Liu,Yanyi Fu,Qinglin Sheng,Jianbin Zheng
Herein, a simple electrochemical sensor for dopamine (DA) detection was developed based on Ag@C core-shell structure and Au nanocomposites (Ag@C/Au). The Ag@C core-shell nanoparticles were synthesized by one-pot hydrothermal method , which were supported on Au nanoparticles in situ reduction process. The as-prepared nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and UV–vis absorption spectra. The electrochemical investigations indicated that Ag@C/Au nanocomposites possessed intriguing properties towards DA. These results could be attributed to the synergistic effect of Ag@C with enhanced substrate accessibility and conductivity, Au nanoparticles with surface of negative charges. Under the optimum experimental conditions, the liner range was 0.5 μM to 27.5 μM, 27.5 μM to 0.2775 mM and 0.2775 mM to 4.278 mM. The detection limit was 0.21 μM (S/N = 3). Furthermore, the electrochemical sensor showed favorable selectivity in the presence of interferents such as ascorbic acid (AA) and uric acid (UA). In general, the work could provide a promising possibility to develop excellent electrochemical sensors for DA detection.
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DYES AND PIGMENTS
·
2021
·
IF 4.889
Guojuan Qu,Tao Jiang,Wenjing Li,Tao Liu,Xiang Ma
Au nanoclusters (AuNCs) as a novel type of luminescent nanomaterial have been extensively explored due to low toxicity, excellent photostability and ultrafine size. However, few progresses have been made in producing ultrabright, homogeneous, functional, water-soluble AuNCs. In this regard, we propose an outstanding cross-linker acrylamide modified [email protected] ( [email protected] ) based on a simple one-step pretreatment , achieving a high quantum yield and self-healing hydrogels by copolymerizing with acrylamide. The plentiful network structure of the hydrogel effectively restricts the vibration of the ligands on the AuNCs surface, resulting in a 4-fold enhancement of quantum yield (from 5.6% to 22.8%). Meanwhile, the prepared [email protected] hydrogel exhibits self-healing capability and excellent elasticity. It is worth noting that AuNCs are used as cross-linkers in the polymerization reaction for the first time, which supplies a platform to realize the functionalization of gold nanoclusters.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Xuemin Xia,Yijin Weng,Lei Zhang,Ruyi Tang,Xia Zhang
Surface-Enhanced Raman Scattering (SERS) is a powerful analysis technology, attracting more and more attention due to its high sensitivity and selectivity. Herein, we report a simple seed-mediated method to synthesize Au@Ag nanoparticles (NPs) as a multifunctional biosensor for the label-free detection of hydrogen peroxide (H 2 O 2 ) and glucose by SERS. Au@Ag NPs, as an ultrasensitive SERS substrate, show the dual activities (peroxidase-like and GOx-like activities). Under the condition of pH 4.0 NaAc buffer solution, the glucose and H 2 O can be catalyzed by Au@Ag NPs to produce glucose acid and H 2 O 2 , and then H 2 O 2 can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue oxidation product oxidic TMB (oxTMB) which exhibits strong SERS signals at 1188, 1330, 1605 cm −1 . Thus, we have developed a new SERS strategy for analysis of glucose with a detection limit of 5 × 10 − 10 mol L −1 , suggesting that Au@Ag NPs have the potential for biosensor, immunoassay and medical treatment.
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Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Wenquan Huang,Yuehua Deng,Liping Ye,Qiuling Xie,Yanbin Jiang
Poor safety and effectiveness is an outstanding challenge in the preparation of drug delivery systems (DDS) for cancer treatment. The pursuit of the high curative effect will inevitably increase the risk of adverse side effects. Herein, a bio-safe DDS was constructed by combining the advantages of functional zein and Au doped mesoporous silica nanoparticles ( [email protected] 2 ) to achieve chemo-photothermal therapy. The cRGD functionalized zein (cRGD-Zein) was coated on the surface of [email protected] 2 which effectively avoided premature leakage of paclitaxel and realized sustained drug release. Meanwhile, the high hemolysis rate (107%) of [email protected] 2 had been significantly reduced to 4%. The anti-hemolysis mechanism of functionalized zein was explored to give a deeper understanding of the interaction between nanoparticles and RBCs. The results showed that the functional zein would change the protein conformation during the interaction with [email protected] 2 to protect the RBCs from the damage of [email protected] 2 . And the release rate of hemoglobin was limited by the size of RBCs membrane cracks with approximately 40 nm in width and 470 nm in length. The cell cytotoxicity and uptake assays showed that the prepared DDS exhibited low tumour cell viability (35%) and enhanced uptake performance (99.3%). This work suggested that the prepared nanoparticles could serve as a promising carrier to achieve safe and efficacious tumour therapy.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yuan Wang,Sen Liang,Mingliang Mei,Qiaowen Zhao,Guangwei She,Wensheng Shi,Lixuan Mu
Detecting the temperature of intracellular mitochondria with high sensitivity and stability is crucial to understanding the cellular metabolism and revealing the processes of mitochondria-related physiology. In this paper, employing the long fluorescence lifetime of modified Au nanoclusters (mAuNCs) by 4-(carboxybutyl) triphenylphosphonium bromide, we developed a fluorescence lifetime thermometer with high sensitivity and stability for the temperature of the intracellular mitochondria. A high relative temperature sensitivity of 2.8% and excellent photostability were achieved from the present thermometer. After incubation with L929 cells, the mAuNCs could be endocytosed into the cells and targeted the mitochondria, and the temperature changes at the L929 cells’ mitochondria, which were stimulated by carbonyl cyanide 3-chlorophenylhydrazone and Ca2+, were successfully detected via the fluorescence lifetime images of the mAuNCs. Furthermore, utilizing the mAuNCs, we clarified the effect of Mg2+ on the temperature of the intracellular mitochondria. The strategy of employing a material with a long fluorescence lifetime and remarkable stability to fabricate the fluorescence lifetime thermometer for mitochondria can be used to design various thermometers for other organelles.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Danli Hu,Haiyan Cui,Xinyang Wang,Fang Luo,Bin Qiu,Weicheng Cai,Hui Huang,Jian Wang,Zhenyu Lin
An Au/GaN photoelectrode was prepared by sputtering 30 nm thick Au film on the surface of n-type gallium nitride (GaN). When the electrode contacts with multilayered molybdenum disulfide (MoS2), photogenerated electrons and photogenerated holes transfer to MoS2 because of the band gap matching of MoS2 and GaN. The presence of Au promotes charge transfer and results in a greater recombination of electrons and holes; by this means, a more significant suppression of photocurrent can be detected. This characteristic has been coupled with the high selectivity of an aptamer and applied to develop a novel photoelectrochemical aptasensor for cancer biomarkers (alpha-fetoprotein (AFP) as a model). The aptamer of AFP was modified on the surface of the Au/GaN photoelectrode by Au–S bonds, which can bind to the target protein with high selectivity. Then, the transfer process of the charge carriers of GaN to MoS2 can be blocked by the target protein so that the suppression of photocurrent is reduced. The difference of the photocurrent in the presence and absence of AFP (ΔI) showed a linear relationship with AFP concentration that ranged from 1.0–150 ng/mL (R2 = 0.9995), and the detection limit was 0.3 ng/mL. The standard addition recovery rates ranged from 85.2 to 91.7%. The method possessed good sensitivity and high selectivity for AFP detection. The developed biosensor can be modified to detect other cancer biomarkers by simply replacing the aptamer used.
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CELL PROLIFERATION
·
2021
·Vol. 54
·期 4
·e13008
·
IF 6.831
Yifang Yuan,Ronghui Zhou,Ting Li,Shuang Qu,Hua Bai,Jiawu Liang,Xiaoxiao Cai,Bin Guo
Objectives Au nanoclusters (AuNCs) have been used widely in fluorescence bio-imaging because of their good fluorescence, small particle size and non-cytotoxicity. AuNCs are also efficient in computed tomography (CT) imaging. Hence, a dual-modal imaging probe can be constructed without any complicated modification processes by exploiting the excellent performance of AuNCs. In the present study, AuNCs were enriched with mesoporous silica nanoparticles (MSNs) to obtain enhanced fluorescence/CT dual-modal imaging, which was capable of acquiring more imaging information for diseases compared with single-mode imaging. Materials and methods Biocompatible bovine serum albumin (BSA)-capped AuNCs were prepared and loaded into amine-functionalized MSNs to form MSN@AuNCs. BSA-AuNCs, MSNs, and MSN@AuNCs were characterized by ultraviolet-visible (UV-vis) spectra, transmission electron microscopy (TEM), fluorescence spectra, and zeta potential. CT imaging was recorded using micro-CT scanning. Fluorescence imaging was measured using confocal laser scanning microscopy and flow cytometry. Results The prepared AuNCs and MSNs possessed good properties as previously reported. The fluorescence intensity and CT value of the AuNCs were enhanced after being enriched with MSNs. The nanoparticles were both non-cytotoxic. Confocal laser scanning microscopy and flow cytometry indicated that MSN@AuNCs in CAL-27 cells showed improved fluorescence imaging compared with simple AuNCs at the same concentration. Conclusions The results revealed that the strategy of enriching AuNCs with MSNs can obtain highly sensitive fluorescence/CT dual-modal imaging, which indicated the potential of this nanoparticle in the diagnosis and treatment of disease.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Gang-gang Zhang,Xiao-cui Lai,Neng-shui Ding,Qi-rong Xiong,Shuai Hou,Hong-wei Duan,Wei-hua Lai
Flower-like gold nanoparticle (AuNF) is widely used as a probe. However, the performance of conventional AuNF is not satisfactory because of complex synthesis, insufficient uniformity, and ordinary optical performance. In this paper, a novel chrysanthemum-like Au@polydopamine (AuNC@PDA) prepared using one-pot method was compared with conventional AuNF@polydopamine (AuNF@PDA) prepared using three-step method. AuNC@PDA had more branch structures with more uniform petaloid shape and narrower particle size distribution compared with AuNF@PDA. The grayscale of AuNC@PDA both in aqueous solution and on nitrocellulose membrane was approximately threefold higher than that of AuNF@PDA. Because of PDA coating, the stability and coupling efficiency of AuNC@PDA were much higher than that of conventional nanoprobes − AuNF and AuNS (gold nanosphere). These results indicated that AuNC@PDA had superior morphological and optical performance. The AuNC@PDA was first utilized as a probe in immunochromatographic assay (ICA), and compared with conventional AuNF@PDA. The limits of detection (LOD) of ICA based on AuNC@PDA, AuNS, and AuNF@PDA were 1.59, 9.58, and 5.13 mIU/mL, respectively, when human chorionic gonadotropin was detected as the target. The LOD of ICA based on AuNC@PDA had 6.0-fold and 3.3-fold improvement compared with that based on AuNS and AuNF@PDA, respectively. Our results could be used as a basis for developing novel probes in practical ICA applications.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 21
·6823-6833
·
IF 7.393
Yingjiao Zhai,Hui Yang,Sinan Zhang,Jinhua Li,Kaixi Shi,Fangjun Jin
The controllability of materials that has attracted widespread attention is a key factor for improving the performance of SERS substrates. Hence, in this study, a kind of controllable materials constructed with Au nano-disk array and monolayer MoS2 had been developed. The optical properties of the composite were studied and the Raman enhancement was obtained. Compared with the monolayer MoS2, the peak of the above composite was slightly red-shifted, and the intensity was increased by about 2.5 times. This was due to the thermal effect and the internal quantum efficiency improvement of MoS2 caused by the local surface plasmon resonance of Au. The simulation results of the finite-difference time-domain and the experimental results all showed an enhanced photoluminescence of the composite, which was attributed to the resonance wavelength of the Au nano-disk being adjusted to be consistent with the photoluminescence peak of the monolayer MoS2. Through the synergistic action of the electromagnetic enhancement and chemical enhancement, the highly sensitive and stable surface-enhanced Raman scattering (SERS) active substrate constructed with Au-MoS2 composite was sequentially utilized in the detection of antibacterial agent residues on the aquatic products. Thereafter, a calibration curve with ultralow limits of detection (LOD) at 10−15 M was established for the standard solutions of crystal violet (CV), and then the assay on shrimps was successfully realized. Therefore, this research provides an effective way for attractive SERS performance of MoS2, and confirms the potential possibility for application on food safety and other trace detection.
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CHEMISTRY OF MATERIALS
·
2020
·
IF 9.567
Changchang Xing,Dilong Liu,Jinxing Chen,Yulong Fan,Fei Zhou,Keerat Kaur,Weiping Cai,Yue Li
The controllable assembly of nanoparticles into nonclose-packed (NCP) arrays exhibits unusual optical properties [e.g., surface lattice resonances (SLRs)] but are challenging to construct, especially for NCP binary arrays composed of diversified nanoparticles. Here, we show the construction of two-dimensional (2D) NCP binary Au nanoparticle arrays by modulating the convective self-assembly of colloidal nanoparticles on a templating NCP nanoparticle array. By dramatically inhibiting the coffee ring effect during convective evaporation, this convective assembly approach allows colloidal nanoparticles to stack around the templating nanoparticles, forming a “core-satellite” basic architecture. Moreover, this approach is generalized for various types, shapes, and periodicities of building nanoparticles. The assembled 2D NCP binary Au arrays attain multiple tunable optical properties, including photonic band gaps, plasmonic SLRs, and dynamic surface-enhanced Raman scattering activity, based on tunable plasmonic coupling. The anticounterfeiting application of these NCP binary arrays is demonstrated by taking advantage of their unique optical properties. This convective assembly approach can pave the way for the development of a new category of NCP binary arrays and help to explore their collective unconventional properties.
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