SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Huanhuan Li,Yu Wu,Xorlali Nunekpeku,Yuhan Yang,Jingsa Zhao,Wei Sheng,Yuexia Wang
The increasing presence of Aflatoxin B 1 (AFB 1 ) residues in food continues to raise significant public health concerns. Herein, we designed an upconversion-based sensor integrated into an alginate-polyacrylamide (SA-PAM) hydrogel for on-site AFB 1 detection in food. First, core-shell upconversion nanoparticles (CSUCNPs) with enhanced fluorescence were modified with complementary DNA (cDNA), forming signal probes known as CSUCNPs-cDNA. Simultaneously, NH 2 -ZIF-8@Cu 2+ with a high surface area and specific affinity for aptamer was synthesized using a one-pot method. This was then modified with an AFB 1 -specific aptamer, forming the capture probe Apt-ZIF-8@Cu 2+ . Using base pairing, CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ were hybridized into sensing probes (CSUCNPs-ZIF-8@Cu 2+ ), which were embedded into SA-PAM hydrogels as stable sensor carrier. Furthermore, the SA-PAM hydrogel showed a reduced swelling ratio of 1.68–1.80 in water and phosphate buffer solution(s) (PBS), which improves stability. In the presence of AFB 1 , the aptamer selectively binds the AFB 1 , increasing electrostatic repulsion between CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ . This disrupts Förster resonance energy transfer (FRET), resulting in a pronounced “signal-on” fluorescence. This sensor achieves AFB 1 diffusion through the SA-PAM hydrogel, FRET disruption and upconversion signal stabilization within 18 min, faster than traditional methods. Under optimized conditions, the sensor exhibited a clear linear correlation between fluorescence intensity and the logarithm of AFB 1 concentration ranging from 0.1 to 100 µg/kg ( R ² = 0.994), with a detection limit as low as 0.08 µg/kg. Besides, its performance was further validated through tests on spiked wheat, corn, peanut, and water, demonstrating high accuracy and reproducibility with relative standard deviation (RSD) below 3.63 % and recoveries of 85.88–98.04 %.
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CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Boyu Tang,Tao Xu,Qingyun Li,Jiayu Tu,Kang Liu,Haiyin Xu,Xiaoling Wang,Yihui Zhou,Gang Yu,Shuang-Feng Yin
Antibiotic and heavy metal contamination in livestock wastewater poses escalating environmental challenges, while conventional adsorption methods are hindered by low efficiency, poor reusability, and potential secondary pollution. In this study, a sustainable sodium lignosulfonate/chitosan/polyvinyl alcohol/poly(acrylic acid) (SLS/CS/PVA/PAA) hydrogel was synthesized by free-radical polymerization using chitosan that had been functionally enhanced with sodium lignosulfonate. The experimental results showed that the hydrogel exhibited high adsorption capacities for levofloxacin (LEV) and Cu 2+ , reaching 1146.73 mg·g −1 and 70.6 mg·g −1 , respectively. The adsorption mechanisms were elucidated through kinetic and isothermal models, fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS). The density functional theory (DFT) calculations reveal that the key binding sites involve -OH and -NH 2 groups of CS and -COOH and -SO 3 H groups. Synergistic interactions between LEV and Cu 2+ , including ion bridging and complexation, significantly enhanced LEV removal. The molecular dynamics simulation confirm that Cu 2+ modulates surface charge via -SO 3 − binding and enhances LEV adsorption through ionic bridging. Moreover, copper recovery was achieved by combustion with a recovery rate of 77.31 ± 1.3 %. This study offers a green and cost-effective strategy for pollutant removal and metal recovery, highlighting the potential of SLS-functionalized chitosan hydrogels in environmental remediation.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Xingtian Zhang,Boyuan Yang,Yong Zhang,Xiaoyu Du,Liwei Hou,Tao Zhou,Hongtao Chu,Wenhui Ma
Polyethylene glycol (PEG) plays a key component in functional materials due to its exceptional water solubility, biocompatibility, and chemical versatility. However, the absence of π-conjugation in PEG's molecular structure prevents fluorescence emission, limiting its utility in fluorescent material applications. In this study, a novel PEG-based sensor ( P ) was synthesized by covalently linking PEG to a coumarin fluorophore. P exhibited significantly enhanced green fluorescence compared to unmodified PEG. It allows rapid (~5 s), highly sensitive, and specific copper (Cu 2+ ) detection through fluorescence quenching, achieving 97.40% quenching efficiency with a 0.52 nM detection limit. Nuclear magnetic resonance ( 1 H NMR) titration, inductively coupled plasma mass spectrometry (ICP-MS), and density functional theory (DFT) calculations confirmed the detection mechanisms, revealing the P –Cu 2+ interaction mechanism. Furthermore, fluorescence imaging results demonstrated that P exhibited a fluorescence quenching response upon the addition of Cu 2+ to HeLa cells, providing a potential new method for detecting Cu 2+ in vivo. In addition, the incorporation of a smartphone color recognition application enables approximate quantification of Cu 2+ concentrations in test samples. This approach not only extends PEG's applicability in sensing and smart technology integration but also offers an efficient, low-cost solution for on-site Cu 2+ detection.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Na Li,Fangrui Yu,Hongbin Li,Xiangrui Meng,Chuanbo Peng,Xueru Sheng,Jian Zhang,Shanshan Liu,Qingwei Ping,Huining Xiao
Low enzyme activity is one of the disadvantages of immobilized laccase. In this study, waste Cu 2+ -loaded activated carbon (Cu-AC) was successfully used in preparing a novel composite support，cellulose / Cu 2+ -loaded activated carbon beads (C / Cu-AC)， and effectively boosted immobilized laccase activity. To achieve optimum conditions for immobilization of laccase, the immobilization time, pH and laccase concentration were examined. The highest immobilized laccase activity (34.21 U/g) was achieved under optimum conditions ( T = 4 h, pH = 4, C = 5 g/L), which was increased by 35.86 % compared to control. In addition, the immobilized laccase showed an outstanding performance in thermostability and reusability compared to free laccase. Moreover, the degradation of BPA by immobilized laccase was carried out, and the optimum degradation conditions were explored. Under such conditions: concentration of BPA was 75 mg / L and pH = 4, t = 1 h, T = 50 °C，the removal yield of BPA reached a maximum of 79.88 %. Therefore, the utilization of waste Cu-CA is a powerful method to boost immobilized laccase activity and creating a new way to high value treatment of waste Cu-CA.
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TALANTA
·
2021
·
IF 6.057
Fei Li,Xiaoxuan Li,Jie Su,Yijun Li,Xiwen He,Langxing Chen,Yukui Zhang
In this work, a novel strategy to prepare molecularly imprinted polymers (MIPs) functionalized magnetic carbon nanotubes (MCNTs) via a facile sol-gel polymerization by adopting Cu 2+ -mediating interaction was presented for selective recognition of celastrol (Cel), in the traditional Chinese medicines (TCM). Firstly, template Cel, 3-aminopropyltriethoxysilane (APTES) as monomer and Cu 2+ (co-monomer) were mixed to form a self-assembled pre-complex, in which Cu 2+ could coordinate with Cel. Meanwhile, APTES plays a role of bridge between APTES and Cel. Secondly, carboxyl modified MCNTs as substrate was added into the pre-complex solution. After that, a multi-step sol-gel polymerization process was occurred in the presence of tetraethylorthosilicate as cross-linker and acetic acid as catalyst. Finally, MIPs layer was formed on the surface of the MCNTs (Cel-MIPs@MCNTs) after the removal of template with methanol/acetic. The morphology and structure of Cel-MIPs@MCNTs was investigated by various characterization techniques. The adsorption performance of Cel-MIPs@MCNTs to Cel was illustrated by kinetic, isothermal and selective binding experiments. The results displayed that the Cel-MIPs@MCNTs possessed fast kinetic equilibrium time (40 s), high adsorption capacity (13.35 μg mg −1 ), good imprinting factor of 3.41, and high magnetic responsivity (44.38 emu·g -1 ), which can be used as an ideal adsorbent for rapid isolation and enrichment of target analytes. A selective and sensitive method based on Cel-MIPs@MCNTs coupling with HPLC was developed for Cel determination including a wide linear range (0.15–200 μg mL −1 ) with correlation coefficient of 0.9998, a low limit of detection (0.05 μg mL −1 ). Furthermore, the applicability of Cel-MIPs@MCNTs was demonstrated to isolate and determine Cel in TCM samples with satisfactory recoveries ranged from 84.47% to 91.5% (RSD<5.35%). The results revealed that Cel-MIPs@MCNTs offer great potential as an adsorbent for selective and efficient isolation of Cel from complex TCM samples.
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INORGANICA CHIMICA ACTA
·
2025
·
IF 3.2
Lu Ren,Jing Wang,Xiangchun Meng,Chang Liu,Ziyi Liao,Dawei Zhang
A novel highly selective and sensitive bis-imidazole fluorescent chemical sensor, 2,6-bis(4,5-diphenyl-1 H -imidazol-2-yl)-4-methylphenol (BMP) was synthesized and utilized for spectral sensing of Cu 2+ . After the addition of Cu 2+ , the green fluorescence of BMP solution quickly quenched, and in the presence of multiple metal ions showed good anti-interference to Cu 2+ , the detection limit was 1.59 nM. Job's plot and 1 H NMR analysis confirm that there is a 2:1 cooperative pattern between BMP and Cu 2+ . DFT calculations at the B3LYP/LANL2DZ/6-311 g level of theory were conducted to explore possible theoretical explanations. The detection of Cu 2+ by this probe has good repeatability under the alternating action of Cu 2+ and EDTA. Test strips made of BMP were used for rapid “naked-eye”detection of Cu 2+ in aqueous medium. The probe was successfully used to monitor Cu 2+ in living cells and real water samples.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Fuyuan Huang,Paner Jiang,Yiliang Chen,Weiwei Wang,Yaxin Han,Chenlu Chen,Guangxin Xiang,Sheng Ye,Laibao Zheng
Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive approach for foodborne pathogen detection; however, a simple, user-friendly, and naked-eye readable readout method remains a challenge. Herein, we synthesized bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs), which exhibit fluorescence quenching upon copper ions (Cu 2+ ) binding and fluorescence recovery in the presence of phosphate ions (PPi). Leveraging this property, we developed a label-free fluorescence turn-on assay for naked-eye LAMP detection by monitoring PPi accumulation under UV light. To mitigate potential cross-contamination risks inherent in LAMP workflows, we integrated the BSA-AuNCs-Cu 2+ complex into the reaction lid, enabling a closed, one-pot detection system. This method demonstrated high sensitivity, detecting Salmonella enterica at concentrations as low as 10 1 CFU/mL within 40 min (excluding nucleic acid extraction process). With its customizable primers and naked-eye visual detection capability, this assay offers a versatile and practical tool for rapid pathogen screening in diverse applications.
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ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Qinghua Gu,Zheng Yan,Xin Ke
To evaluate the potential of aerogel materials for selectively recovering Cu 2+ from highly acidic wastewater, the adsorption behavior of Cu 2+ by bis-pyridine N-functionalized cellulose (PEIPD/CMC) aerogel was systematically investigated. Characterization of structural and chemical properties of the absorbent revealed that the bis-pyridine N plays a crucial role in the adsorption process. The PEIPD/CMC aerogel exhibited excellent adsorption capacity and selectivity for Cu 2+ in multi-component acidic solutions (Cu 2+ /Zn 2+ /Pb 2+ ), achieving a maximum adsorption capacity of 1.19 mmol/g at pH = 2. The adsorption process was well-described by the pseudo-second-order model and the Langmuir model, indicating monolayer chemisorption. Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses confirmed the involvement of bis-pyridine N groups in the adsorption of heavy metals. To further elucidate the interaction mechanisms between PEIPD/CMC and heavy metals at the microscopic level, Molecular dynamics (MD) simulations and Density functional theory (DFT) were conducted. MD simulations revealed that the adsorption process is predominantly governed by chemisorption, with the binding strength of the adsorbent to heavy metals following the order Cu 2+ > Zn 2+ > Pb 2+ . DFT calculations provided direct evidence of the chelation mechanism, highlighting the role of energy and electron transfer in the selective adsorption of Cu 2+ . These findings conclusively demonstrate that bis-pyridine N is the key functional group responsible for the selective adsorption of Cu 2+ .
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Hong Huang,Shuai Li,Biyun Chen,Yuan Wang,Zhangfeng Shen,Ming Qiu,Hu Pan,Weikang Wang,Yangang Wang,Xi Li
Endoplasmic reticulum (ER) is the largest organelle in eukaryotic cells and plays a variety of functions in living cells include protein folding, calcium homeostasis, and lipid biosynthesis. Normal function of ER is crucial for cell survival, while disequilibrium of ER can cause misfolding of proteins and ER stress, leading to many serious diseases. It has been documented that ER stress is closely related to the metabolism of Cu 2+ , as ER is the main intracellular accumulation space of Cu 2+ and toxic reactive oxygen species can be generated by Cu 2+ via Fenton and Haber-Weiss reactions. In this context, developing a powerful tool capable of selective and sensitive monitoring of Cu 2+ in ER and investigating its role in physiological and pathological processes is of great importance. Herein, we report the first ER targeted near infrared (NIR) nanosensor, polymer dots encapsulated with NIR hydrophobic carbon nanodots, for detecting Cu 2+ in biosystems. This nanosensor with stable fluorescence showed a fast response toward Cu 2+ (120 s) and can be used for the quantification of Cu 2+ in a linear range covering from 0.25 to 9.0 μM with a detection limit of 13 nM. In addition, the fluorescence variations of the nanosensor are remarkably specific to Cu 2+ in comparison with the other metal ions and amino acids. Moreover, the developed nanosensor exhibited low cytotoxicity, good biocompatibility, and ER targeting ability. Because of these excellent spectroscopic features, the nanosensor was successfully utilized for visualizing Cu 2+ fluctuations at the living cell, zebrafish and mouse levels, which further proved its potential application in biological systems.
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Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2023
·
IF 2.5
Chenchen Ma,Zihan Ma,Ziyuan He,Xianjian Wang,Longhao Zhao,Xuegang Chen
Novel hydrophilic polymers consisting of fluorene modified by alkoxyl chain were designed and synthesized via radical polymerization from acrylic acid (PAAF) or acrylamide (PAMF) in the presence of azobisisobutyronitrile. All of these polymers were good water-soluble. The photophysical behaviors of the polymers and the selectivity and sensitivity to detection metal ions were studied by UV-visible and fluorescence spectroscopy technique, and some color changes were visible to the naked eye appeared. The PAMF fluorescent probe showed good selectivity and sensitivity to detection Fe3+ (2.4 × 10−7 mol/L) and PAAF exhibited good selectivity and sensitivity to detection Fe2+ (1.5 × 10−6 mol/L) and Cu2+ (5 × 10−7 mol/L) in water.
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Desalination and Water Treatment
·
2024
·
IF 1
Chunping Liu,Yongrui Pi,Guodong Ju,Zhaozhao Wang
A low-cost adsorbent, tea waste was activated by nitric acid and adsorption of Cu 2+ and direct sky blue 5B from aqueous solution was investigated. Response surface methodology (RSM) was used to study the adsorption process. The RSM results showed that temperature and concentration are major factors to modelling Cu 2+ and direct sky blue 5B adsorption, respectively. Higher temperature has a positive effect on the adsorption of Cu 2+ and direct sky blue 5B. Under the same temperature, the adsorption capacity was increased along with the concentration till maximum is attained both for Cu 2+ and direct sky blue 5B. The optimum condition of the RSM for Cu 2+ was determined as following: pH 4.5, 45°C, concentration of 1.5 × 10 –2 mol/L; for direct sky blue 5B, the optimal condition were pH 6.0, 25°C, 1.5 × 10 –2 mol/L. The equilibrium data during adsorption were modeled to Langmuir and Freundlich isotherms for Cu 2+ . For direct sky blue 5B, the equilibrium data were well presented by Freundlich isotherms. The maximum adsorption capacities were determined at 59.88 mg/g for Cu 2+ , 58.27 mg/g for direct sky blue 5B. The kinetic data fit to pseudo-second-order equation well with correlation coefficients larger than 0.99. The results strongly support that tea waste can be used as an economic and excellent adsorbent for the removal of Cu 2+ and direct sky blue 5B from contaminated water.
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ANALYTICAL CHEMISTRY
·
2026
·Vol. 98
·期 31
·23335-23345
·
IF 7.3
Dong Hui,Lv Xiaoyu,Qian Yujia,Chang Aixin,Cheng Menghao,Yang Xue,Ding Jiahui,Yang Zimo,Zhou Yanli,Zhang Yintang,Li Zhaohui,Xu Maotian
Copper ions (Cu 2+ ) are
closely implicated in the pathogenesis
of Alzheimer’s disease (AD) by mediating oxidative stress and
promoting β-amyloid (Aβ) aggregation. Accurate quantification
of labile Cu 2+ in the brain extracellular microenvironment
is critical for elucidating its pathological roles and developing
AD diagnostic tools. Herein, we report a dual-mode sensing platform
integrating “turn-on” fluorescence and ratiometric electrochemistry
for sensitive and selective Cu 2+ quantification, based
on a resorufin-picolinate conjugate (RP) probe and a carbon nanotube-modified
carbon fiber microelectrode (CFME/CNT). Resorufin (Re) acts as both
the fluorescent reporter and electrochemical redox center, while picolinate
serves as the Cu 2+ -specific recognition moiety. Cu 2+ coordinates with picolinate to catalyze the ester hydrolysis
of RP, releasing free Re that triggers a fluorescence enhancement
at 594 nm and a ratiometric electrochemical response, corresponding
to the oxidation currents of free Re and intact RP, respectively.
The RP probe exhibits high selectivity for Cu 2+ over other
metal ions, with limits of detection of 19 nM (fluorescence) and 7.2
nM (electrochemistry). The dual-mode platform was successfully applied
to the accurate quantification of endogenous labile Cu 2+ in cerebral cortex dialysate from AD mice and age-matched wild-type
(WT) mice, and noninvasive in vivo fluorescence imaging further confirmed
the elevated Cu 2+ level in living AD mouse brains. Quantitative
results revealed that the labile Cu 2+ concentration in
AD mouse cerebral cortex dialysate was significantly higher than that
in WT mice. The two detection modes yielded highly consistent results,
validating the reliability of the dual-mode platform in complex biological
matrices. This dual-mode strategy synergizes the merits of fluorescence
and ratiometric electrochemistry, providing a robust and selective
tool for investigating AD pathogenesis related to Cu 2+ dyshomeostasis
and holds great potential for clinical translational applications
in AD diagnosis.
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