Scientific Reports
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2017
·Vol. 7
·期 1
·1-10
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IF 4.259
Chu Qiang,Zhao Yiying,Shi Xuer,Han Wen,Zhang Yanzhen,Zheng Xiaodong,Zhu Jing
To address the need for a high throughput toxicity test in the modern food industry, an in vivo -like 3-D cell model was constructed in this study to provide an alternative to controversial long-term animal models and to improve the sensitivity and accuracy of the traditional monolayer model. The model formed cell cylindroids within polyvinylidene fluoride (PVDF) hollow fibers and therefore mimicked the microenvironment of liver tissue. Microscopy methods were used, and liver-specific functions were measured to demonstrate the superiority of the model compared to the monolayer model, as well as to optimize the model for best cell performances. Later, toxicity tests of sodium nitrite and acrylamide were conducted in both the 3-D model and the monolayer model to study the sensitivity of the 3-D model in toxicity responses. As expected, HepG2 cells within the 3-D model responded at lower concentrations and shorter exposure times compared to cells within the monolayer model. Furthermore, western blot analysis of apoptosis pathways also supported the argument.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Chuntao Yang,Kaiyong Feng,Jiangchang Chen,Ze Liu,Yingjian Yu
The commercial applications of aqueous rechargeable zinc-ion batteries (ZIBs) remain limited mainly owing to the low capacity storage efficiency and structural deformation of cathode materials, coupled with anode dendrite formation and side reactions that compromise battery performance. Herein, the electronically conductive metal-organic framework (EC-MOF) DDA-Cu was developed and used as the cathode for the first time in ZIBs. The electrode material showed an excellent initial discharge capacity of 249.6 mAh g −1 at 0.2 A g −1 . Notably, it also reached a effective capacity of 120 mAh g −1 after 175 cycles at an increased current density of 1 A g −1 . When used as an interface protective coating for zinc anodes, the uniform pore channels of DDA-Cu facilitated homogeneous zinc-ion deposition, which effectively suppressed dendrite growth and side reactions, typically a hydrogen evolution reaction (HER). Compared with bare zinc anodes, the Zn@DDA-Cu anode significantly extended battery lifespan, demonstrating stable cycling for over 3500 h at 0.5 mA cm −2 . Notably, the full battery with a Zn@DDA-Cu anode and a DDA-Cu cathode exhibited stable cycling for 100 cycles at 1 A g −1 , retaining a capacity of 185.5 mAh g −1 . This study provides an innovative strategy for the development of promising EC-MOFs for practical ZIB application.
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Journal of Functional Foods
·
2024
·
IF 3.8
Xi-you Li,Xiao-yuan Gu,Bai-ling Zhou,Bun Tsoi,Wei-xi Li
Eye deformity, a noteworthy embryopathy, is intricately linked with maternal diabetes, frequently resulting in embryonic malformation or even death. In this investigation, a hyperglycemic chick embryo model was established to delve into the protective potential of tamarind shell extract (TSE) on eye development. Chick embryos were administered with TSE (at dosages of 1, 2, and 4 µg/egg) through air sac injection on embryo development day 0 (EDD0) and later exposed to D-glucose (0.4 mmol/egg) on EDD1. To validate our findings, we replicated hyperglycemic environment using D-glucose in ARPE-19 cell model. Our research unveiled that TSE proficiently counters O-GlcNAcylation of proteins in the presence of D-glucose, thereby reinstating levels of key proteins involved in eye development, including Pax6, Daam1, mTOR, and Cdc42. Furthermore, TSE was pinpointed to curtail the D-glucose concentration within the embryo eye, considerably decreasing the accumulation of reactive oxygen species and malondialdehyde accumulation in both the ARPE-19 cells and chick embryos. Beyond its fundamental role in boosting the cellular antioxidative activity, the improved expression of apoptosis-related proteins like Caspase-3, BAX, BCL-2 and other like PI3K and AKT were observed. This suggests a probable activation of the PI3K-AKT-mTOR pathway on ARPE-19 cells by TSE, thereby downregulating apoptotic proteins in ARPE-19 cells during oxidative stress. TSE may become a potent natural countermeasure against D-glucose-induced eye malformation.
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JOURNAL OF CATALYSIS
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2025
·
IF 6.5
Chengliang Wang,Xia Guo,Jinbian Lv,Yin Zhang,Haitao Li,Jie Gao,Wei Wei,Di Zhang,Yongxiang Zhao
The primary products of the direct hydrogenation of Maleic Anhydride (MA) are Succinic Anhydride (SAH) and γ-butyrolactone (GBL). Achieving selective synthesis of these products by regulating the composition, structure, and textural properties remains a focal point in the research field of maleic anhydride hydrogenation. Based on the characteristic of atomic level uniform distribution of cations of layered double hydroxides (LDHs), a highly loaded, highly dispersed and homogenized NiCu alloy bimetallic catalyst was synthesized using NiCuAl-LDHs as precursor. Compared with Ni/Al 2 O 3 catalyst derived from NiAl-LDHs, the introduction of Cu can effectively improve the hydrogenation activity of C=C bonds while inhibiting the hydrogenolysis of C=O bonds. Under identical reaction conditions, the primary product of the Ni/Al 2 O 3 catalyst was γ-butyrolactone (GBL) with a yield of 94.2 %, whereas the primary product of the NiCu 0.4 /Al 2 O 3 catalyst was Succinic Anhydride (SAH) with a yield of 90.5 %. The introduction of Cu resulted in the formation of a homogeneous NiCu alloy phase in the catalyst, which significantly influenced the adsorption configurations and adsorption energies of the reactants and intermediates. This effect can be attributed to the electronic interactions between Ni and Cu, which shift the d-band center of Ni away from the Fermi energy level, thereby facilitating the directed synthesis of Succinic Anhydride (SAH) and γ-butyrolactone (GBL). These findings were confirmed by various characterization techniques and DFT theoretical calculations. Future catalyst development in maleic anhydride hydrogenation will significantly benefit from the design approach and research ideas presented in this study.
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ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Yanan Deng,Zihao Zhang,Yuanhao Pang,Xueying Zhou,Yu Wang,Yun Zhang,Yali Yuan
The poly-L-cysteine modified Au nanoparticles (Au@p-L-Cys) were constructed on electrode surface as a highly efficient chiral interface for tryptophan (Trp) enantiomers recognition via one step electropolymerization. With the aid of Cu 2+ , L-Cys residues and D-Trp target formed a sandwich complex D-Trp-Cu 2+ -L-Cys, while L-Trp was unable to form such complex due to the steric hindrance provided by the chiral interface, which was confirmed by the electrochemical and SEM results. With the introduction of ferricyanide probe, D-Trp produced significant current decrease while L-Trp produced a slight current increase, which implied the successful enantioselective recognition of Trp enantiomers (specifically D-Trp) in the true sense. This novel sensor showed a surprisingly wide linear range toward D-Trp of 6 × 10 −7 M to 1 × 10 −2 M, with a detection limit as low as 75 nM (S/N = 3). Moreover, the exclusive enantioselectivity toward D-Trp was discovered since other amino acids showed negligible interference to detection of D-Trp. The recovery of D-Trp in human serum was between 91.30 and 109.3%, which further verified the satisfying specificity and practicality of the proposed strategy. The coordination thermodynamics by UV–Vis spectroscopy and DFT simulation were also used to investigate the enantioselective mechanism. These results highlight the great potential of using Au@p-L-Cys to construct chiral interface for enantiomers recognition and hold the promise of practical application of electrochemical chiral sensors in fields like pharmaceutics and bioanalysis .
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2014
·
IF 3.552
Qiang Zhang,Qinfu Zhao,Ying Zhang,Ning Han,Liang Hu,Chen Zhang,Tongying Jiang,Siling Wang
3-D ordered macroporous (3DOM) materials were customized for BSA adsorption and further oral immunization. These carriers have a high adsorption capacity and our customized carrier showed a distinctive double-plateau adsorption behavior. Different BSA release rates (between the two plateaus) could be obtained by adjusting the ratio of the protein adsorbed on the internal surface and the external surface. This suggests that the release pattern was determined by the adsorption state. One benefit is that the same carrier could have different release profiles making it possible to study the relationship between the release behavior and adjuvant effects without any distractions. Compared with free BSA alone, a significantly higher level of serum IgG, IgA induced by BSA/3DOM was observed and the release profile had an effect on the immunity. The IgG1 and IgG2a titers suggesting that both the Th1 and Th2 mediated immune response were induced. Therefore, this research could help in the development of a novel inorganic oral adjuvant and provide a new avenue for the administration of oral vaccine.
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Food Bioscience
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2025
·
IF 5.9
Chenxi Gu,Liyan Yang,Susu Han,Dengfeng Yang,Xiaodong Xu,WenChao Zhang,Mingguo Jiang,Lixia Pan
Cold-active chitinases that maintain robust catalytic performance at low temperatures are gaining attention as eco-friendly biocatalysts for energy-saving bioprocesses. In this study, a novel multifunctional chitinase of GH18 ( Cm Chi18B) was successfully cloned from a newly identified mangrove-associated bacterium Chitinibacter mangrovi FCG-7 T in Escherichia coli . The enzyme Cm Chi18B demonstrated adaptability to cold environments, retaining 55% of its maximal activity at 0°C. It displayed optimal activity at 55°C and pH 5.5. Cm Chi18B demonstrated high activity across a broad pH range of 4.0-12.0. Substrate hydrolysis analysis indicated that Cm Chi18B possesses bi-functional capabilities, including exochitinase and N-acetyl-β-D-glucosaminidase activities. After 24 h of incubation with colloidal chitin at 30°C, the enzyme achieved a N-acetyl-D-glucosamine (GlcNAc) conversion rate of 91.15% with a purity exceeding 98%. Intriguingly, the hydrolysis time for GlcNAc production varied with temperatures, with 0°C significantly impairing N-acetyl-β-glucosaminidase activity. These attributes position Cm Chi18B as a promising, low-energy biocatalyst for the sustainable production of GlcNAc and the valorization of chitin waste streams.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2014
·
IF 2.711
Xiaomin Xia,Chuanyang Su,Juanli Fu,Pu Zhang,Xiaoji Jiang,Demei Xu,Lihua Hu,Erqun Song,Yang Song
This study investigated the protective effect of α-lipoic acid (LA) on lipopolysaccharide (LPS)/ d -galactosamine ( d -GalN)-induced fulminant hepatic failure in mice. First, we found that LA markedly reduced LPS/ d -GalN-induced increases in serum ALT and AST activities, which were supplemented with histopathological examination, suggested that LA has a protective effect on this model of hepatic damage. Livers challenged with LPS/ d -GalN exhibited extensive areas of vacuolization with the disappearance of nuclei and the loss of hepatic architecture. On the contrary, these pathological alterations were ameliorated by LA treatment. Next, we found that ROS and TBARS levels were increased in LPS/ d -GalN treated liver homogenates , which were attenuated by LA administration. Consistently, decreases in hepatic CAT and GPx activities were observed in LPS/ d -GalN group and were significantly restored by LA administration. Moreover, pretreatment with LA markedly reduced LPS/ d -GalN-induced iNOS , COX-2, TNF-α, NF-κB, IL-1β and IL-6 expressions. Furthermore, our data showed that TUNEL-positive cells increased in LPS/ d -GalN-treated mice liver which was counteracted by LA administration. LPS/ d -GalN induced apoptosis of hepatocytes, as estimated by caspase 3, caspase 8 and caspase 9 activations. Also, the increasing of Bax and the decreasing of Bcl-2 expressions also supported LPS/ d -GalN induced apoptosis. Interestingly, LA marked relieved these apoptotic features. Taking together, our results indicated that LA plays an important role on LPS/ d -GalN-induced fulminant hepatic failure through its antioxidant, anti-inflammatory and anti-apoptotic activities.
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DYES AND PIGMENTS
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2025
·
IF 4.2
Zewei Song,Shuang Li,Yijie Zou,Yinghan Li,Xun Sun,Shouzhi Pu,Zhao Chen
Three D-A-A′ architecture isomeric luminogens ( o -, m -, p -DMAc-Ph 2 CO-BT ) were designed and synthesized, comprising acridine (donor, D), benzophenone (acceptor, A), and trifluoromethylphenol (acceptor, A′) units. Impressively, all three prepared compounds achieved concentration-dependent high-quality white-light emission, which resulted from the integration of bluish-violet single molecules and yellow excimers. Furthermore, solid p -DMAc-Ph 2 CO-BT exhibited reversible mechanofluorochromism (MFC), with a high-contrast fluorescence shift from blue-green to yellow-green upon grinding, rendering it suitable for a rewritable medium. Single-crystal structural analysis revealed the underlying mechanism of force-responsiveness among the isomers that only o -DMAc-Ph 2 CO-BT possessed C–H⋯F interactions, accompanied by diverse and shorter intermolecular contacts, leading to rigid stacking and MFC passivity. In contrast, p -DMAc-Ph 2 CO-BT showed the weakest intermolecular interactions and the most loosely packed structure, endowing it with high sensitivity to mechanical force. This study demonstrated the critical role of intermolecular interactions in modulating the MFC, providing valuable insights for the rational design of intelligent fluorescent materials.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
·
IF 9.1
Zhaoguo Zhang,Dan Mei,Noor Ul Afsar,Yanan Chen,Jin Tang,Ke Liu,Jawad Ali,Dong Wang
Advanced membrane design with high proton selectivity is essential in industry for effective acid recovery and the elimination of multivalent metal ions, thereby reducing chemical consumption and environmental impact. Here, we present Vinyl carbazole (Vc)-functionalized membranes with adjustable interchain spacing (d-spacing) and hydrophilic channels to enhance acid recovery via diffusion dialysis. Adjusting Vc content optimizes the membrane structure for balanced proton conduction and metal-ion transport rejection. Moderate Vc incorporation (2–8 mol%) expands interchain spacing and simultaneously facilitates in situ crosslinking, thereby forming dense networks that suppress water uptake. This form of interconnected ionic channels significantly increases U acid flux: U acid values are 18.21 × 10 −3 m h −1 (HCl/FeCl 2 ) and 14.02 × 10 −3 m h −1 (H 2 SO 4 /FeSO 4 ), while metal ion transport (U salt ) is suppressed to 2.85 × 10 −6 m h −1 (HCl/FeCl 2 ) and 4.5 × 10 −6 m h −1 (H 2 SO 4 /FeSO 4 ). In the HCl/FeCl 2 system, the membrane achieved high selectivity (S ≈ 997.81–4515.99). However, further increasing the Vc content up to 10 mol% (PT 0.9 -Vc 0.1 ) enhances crosslinking, which reduces selectivity (S ≈ 1612.59) and proton flux (U acid = 9.20 × 10 −3 , and 8.86 × 10 −3 m h −1 for HCl/FeCl 2 and H 2 SO 4 /FeSO 4 ). These findings suggest that moderate Vc levels optimize high H + conductivity and prevent metal-ion crossover through steric effects, Donnan exclusion, and charge-assisted hopping via the carbazole nitrogen, thereby controlling Fe 2+ crossover. The adjustable microstructure also minimizes swelling, creating durable membranes suitable for industry use.
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GREEN CHEMISTRY
·
2026
·
IF 9.2
zonghao xu,Zongyu Guan,Yan Song,Xiran Zhao,Yaodong Yuan,Zhipeng Yan,Junhang Tian,Xueyi Sun,Biwei Xiao,Weidong Zhuang
Direct recycling of spent LiFePO₄ (LFP) cathodes is a sustainable and energy-efficient strategy for recovering valuable materials while preserving the intrinsic olivine structure. Herein, we report a LiI-assisted regeneration approach that achieves electronic structure reconstruction and reinforced Fe–O d–p orbital hybridization, enabling simultaneous repair of structural defects and enhancement of Li⁺ transport kinetics. The reductive I⁻ ions promote Fe³⁺→Fe²⁺ conversion and effectively eliminate Fe–Li antisite defects, while Li⁺ replenishment restores the stoichiometry of degraded LFP. Density functional theory (DFT) calculations reveal that LiI triggers a downward shift of the Fe 3d band center and an upward shift of the O 2p band center, narrowing their energy separation and strengthening Fe–O covalency. These electronic modifications reduce Li⁺ migration barriers and reconstruct continuous Li⁺ transport channels, as further validated by ab initio molecular dynamics simulations. In-situ XRD measurements confirm reduced unit-cell volume fluctuations and enhanced FeO₆–PO₄ framework stability, demonstrating improved structural reversibility during cycling. Benefiting from this coupled structural–electronic reconstruction, regenerated LFP delivers 146.9 mAh g⁻¹ at 1 C and retains 97.4% capacity after 500 cycles, outperforming the spent material. This work proposes a green and low-energy direct regeneration route for cathode materials and provides important theoretical guidance for their efficient repair and regeneration.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2026
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IF 21.1
You Xu,Huizhen Wang,Jiabing Geng,Yufeng Yuan,Kai Deng,Hongjie Yu,Ziqiang Wang,Hongjing Wang,Liang Wang
Electrosynthesis of cyclohexanone oxime (CYCO) from nitrite (NO 2 ⁻) and cyclohexanone (CYC) offers a sustainable alternative for green CYCO production. However, precise control of the NO 2 ⁻ reduction pathway to generate *NH 2 OH intermediates while suppressing CYC/CYCO hydrogenation and hydrogen evolution reaction remains challenging. Here, an intermetallic PdInBi metallene ( i -PdInBiene) catalyst was designed for CYCO electrosynthesis, achieving nearly 100% yield and an optimal Faradaic efficiency of 40.73 ± 2.74%. Combination of in situ characterizations with theoretical calculations reveal that the asymmetric p-d-p orbital hybridization in atomic-scale ordered configuration could regulate the local electronic structure of active sites and induce short pathway-hydrogen spillover. The *H modulation over specific active sites favors the selective reduction of NO 2 ⁻ to *NH 2 OH, promotes its spontaneous coupling with CYC to produce CYCO, while suppressing undesired side reactions. The system’s economic feasibility and broad substrate applicability further highlight its potential as a mild, sustainable strategy for oxime electrosynthesis.
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