Bioresources and Bioprocessing
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2026
·Vol. 13
·期 1
·57
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IF 6.3
Liu Bohua,Guo Qingqing,Wang Shuo,Shi Ting,Lu Fuping,Zhang Yi-Heng P. Job
Trehalose 6-phosphate (T6P), often referred to as plant insulin, serves as a central signaling molecule that regulates carbon partitioning and sucrose flux in plants, thereby influencing key agronomic traits, such as grain yield and drought resilience. Foliar spraying of T6P has been shown to significantly enhance yield and stress tolerance of numerous grains and vegetables. To circumvent the dependency on coenzymes in natural T6P synthesis, we designed and validated an in vitro new-to-nature, coenzyme-free, minimal enzymatic pathway for the biosynthesis of T6P from maltose and polyphosphate. This three-enzyme cocktail contained maltose phosphorylase, trehalose 6-phosphate phosphorylase, and polyphosphate glucokinase, and did not involve any costly coenzymes, such as ATP or UDP. Through systematic optimization of experimental parameters (including pH, temperature, Mg²⁺, phosphate concentration, and enzyme ratios), a 93% molar yield of T6P was achieved from 10 g/L maltose. The scale-up of this in vitro bioprocess to a 100-mL bioreactor with 200 g/L maltose enabled the production of up to 541 mM T6P (i.e., 252 g/L T6P disodium salt) within two hours, corresponding to a very high volumetric productivity of 126 g/L/h. This study established a scalable and cost-competitive in vitro biomanufacturing of T6P. Chemical intervention based on timed foliar spraying of T6P to cultivated crops offers a simpler and safer agricultural practice compared to genetic modification of crops. Graphical abstract The alternative text for this image may have been generated using AI.
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2026
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IF 1.7
Wang Guohua,Luo Xu,Tian Jie,Lv Wenbo,Zhang Jiale,Yi Zhuhu,Tong Jun,Li Shiyou
In this study, a novel phosphorus-doped kitchen waste biochar (P-KWBC) was prepared through sodium phosphate-assisted copyrolysis. This simple and low-cost adsorbent achieved a remarkable uranium adsorption capacity of 655.13 mg/g under weakly acidic conditions (pH = 4, 313.15 K), which was more than 500% greater than that of kitchen waste biochar. After five consecutive adsorption‒desorption cycles, the material maintained a high uranium removal efficiency of 90.93%. Quantitative mechanism analysis confirmed that ion exchange (41.09%), mineral precipitation (28.23%), π–π interactions (19.69%), and coordination (10.97%) dominated U(VI) removal. This work provides a waste-to-wealth strategy for simultaneous kitchen waste reuse and uranium-contaminated wastewater treatment.
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RESEARCH ON CHEMICAL INTERMEDIATES
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2026
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IF 3.3
Zou Yutong,Zhao Jingyuan,Li Haixia
Antibiotic contamination in water bodies has necessitated effective and sustainable wastewater treatment strategies. In this study, durian shell-derived activated carbon (DSAC) was synthesized via pyrolysis combined with KOH activation, and its adsorption performance for antibiotics from aqueous solutions was systematically investigated. DSAC was characterized by FTIR, XPS, Raman, XRD, and BET. The results revealed that DSAC-3 exhibited a high surface area (3008 m 2 g −1 ) and a well-developed porous structure. This study investigated the effects of pH, initial antibiotic concentration, adsorbent dosage, and temperature on the adsorption capacity of DSAC-3 for CHL and TC. The maximum adsorption capacities for TC and CHL calculated by the pseudo-second-order kinetic model reached 581.7 mg g −1 and 425 mg g −1 , corresponding to removal efficiencies of 78% and 45%, respectively. Thermodynamic parameters indicated the spontaneity and exothermic character of the adsorption process. The plausible adsorption mechanism was proposed to be primarily dominated by pore-filling, π-π EDA interactions, and van der Waals forces, with hydrogen bonding and electrostatic interactions as secondary contributors. These findings suggest that DSAC demonstrates great potential as an effective, low-cost adsorbent for antibiotic removal from contaminated water.
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Nature Communications
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2026
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IF 18.1
Liu Chengfei,Lei Chuxin,Shen Shuting,Jiang Hao,Li Mingyang,Qi Pengfei,Wang Yan,Fan Wenxin,Sui Kunyan,Yu Guihua
Interfacial solar vapor generation (SVG) has emerged as a promising strategy for water purification and desalination. Among SVG technologies, hydrogel-based evaporators stand out for their high energy efficiency and effective evaporation, but their performance often collapses under high irradiance due to insufficient water transport. Here we develop an ultralow-density rigid-network (ULR) hydrogel engineered for ultrafast water transport and evaporation. The ULR network maximizes water content and establishes steep osmotic-pressure gradients for rapid water supply, while the rigid, anti-shrinkage framework preserves hierarchical pores, sustaining capillary-driven flow and maintaining continuous vapor production under extreme irradiance. ULR evaporators surpass traditional hydrogels’ theoretical maximum water transport rate, achieving an evaporation rate of 25.57 kg m⁻² h⁻¹ at 10 suns for 100 h. In practical trials, a low-cost module produced 138 L m⁻² day⁻¹, yielding 12.42 L day⁻¹ of potable water. This design advances hydrogel-based SVG toward robust, affordable solutions for real-world water scarcity.
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MICROCHIMICA ACTA
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2026
·Vol. 193
·期 5
·357
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IF 5.4
Yang Zhu,Gama Mohamed I. H.,Abdullahi Saminu,Ali Mubashir,Chieng David,Kwong Chiew-Foong,Kar Pushpendu,Nie Zedong
A wearable epifluidic patch featuring a hierarchical dual-lock mechanism is introduced. Tetrahedral DNA nanostructures co-localize enzymes at defined spacing to promote substrate channeling and accelerate kinetics, while a gelatin hydrogel stabilizes the enzymatic microenvironment during hydration cycles, ensuring uniform reaction conditions for homogeneous signal formation. This design produced a visible glucose-responsive signal within 30 s, with a linear range of 50–400 µM and a detection limit of 9.98 µM. Integrated with passive microfluidics and smartphone imaging, the patch enables sequential sampling in human trials conducted during physical exercise. The hierarchical immobilization method, device design, and system integration outlined demonstrate a platform for intuitive, electronics-free colorimetric sensing in dynamic environments. Graphical abstract The alternative text for this image may have been generated using AI.
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Nano-Micro Letters
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2026
·Vol. 18
·期 1
·346
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IF 38.5
Chen Jiajie,Huang Jimin,Yang Zhibo,Tang Kai,Wu Chengtie,Ye Huamao,Shi Jianlin,Zhu Yufang
Diabetes aggravates postoperative tumor recurrence and impairs wound healing due to divergent metabolic adaptations to hyperglycemia in tumor versus normal cells. Current therapeutics fail due to the incapabilities in adaptively modulating the metabolic bifurcation across the contradictory pathological contexts. Here, we address this challenge by developing a platinum (Pt) single-atom nanocatalyst (PtSNC) with microenvironments-selective multienzyme-mimicking activities to pioneer a bi-polar bioenergetic intervention strategy. In acidic and reduced nicotinamide adenine dinucleotide (NADH)-overexpressed tumor niches, it exhibits NADH oxidase (NOX)-, oxidase-, and peroxidase-like activities, depleting NADH reserves and generating highly reactive Pt = O species to disrupt energy metabolism and induce both apoptosis/ferroptosis. While it shows NOX-, catalase-, and superoxide dismutase-like activities in neutral diabetic wounds, rectifying hyperglycemia-induced cellular NAD + /NADH abnormity and bioenergetic disorder to revitalize the cells and tissues, with promoting angiogenesis and mitigating local inflammation to accelerate regeneration. Murine diabetic melanoma resection models demonstrate its extensive capacity in effectively eradicating residual tumor tissues and suppressing the recurrence, and promoting diabetic wound healing concurrently without systemic toxicity, making PtSNC a safe and potent nanotherapeutic for diabetic tumor postoperative therapy. This study holds promise for the application of single-atom catalytic medicines in precision therapy for intractable diseases featuring pathological metabolic bifurcation. The alternative text for this image may have been generated using AI.
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JOURNAL OF MATERIALS SCIENCE
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2026
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IF 4.4
Liu Tong,Zhang Jie,An Qianqian,Wu Yusheng,Li Laishi,Fang Tiehui,You Junhua
Electrocatalytic water splitting is a clean and sustainable method for hydrogen production. Cobalt diselenide (CoSe 2 ) is an electrocatalyst with great application prospects. However, due to the inherent low electrical conductivity and aggregation tendency of transition metal selenides, their practical applications are limited. In this study, under the constraint of three-dimensional porous nitrogen-doped reduced graphene oxide/nickel foam (N-rGO/NF) substrates, small-sized nitrogen-doped cobalt diselenide (CoSe 2-x N x ) nanoneedles were prepared by a one-step hydrothermal method, and excellent electrocatalysts were obtained. The optimized catalyst (N-rGO/CoSe 2-x N x /NF-100) exhibits excellent OER performance in an alkaline environment at current densities of 10, 50 and 100 mA cm −2 . N-rGO/NF not only provides good electrical conductivity for the electrocatalyst, but also limits the size of CoSe 2 . The three-dimensional porous structure constructed by small-sized CoSe 2 nanoneedles increases the specific surface area of the electrocatalyst, which is conducive to the OER process. The doping of nitrogen in CoSe 2 forms Co-Nx electron transfer channels, accelerating charge transfer. Density functional theory (DFT) calculations have confirmed that structural modification methods such as limiting the size of the electrocatalyst to avoid agglomeration, as well as electronic control methods such as nitrogen doping, can accelerate the surface structure reconstruction process of the catalyst, thereby improving the adsorption energy of the electrocatalyst for OER intermediates and reducing the overpotential.
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Nature Communications
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2026
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IF 18.1
Lu Shaojie,Hu Yiping,Li Zhenwen,Zheng Liyu,Xiong Lei,Yue Qin
Developing highly efficient noble-metal-free catalysts for oxygen reduction reaction (ORR) is critical for the widespread commercialization of metal-air batteries. Facet engineering provides an effective strategy to optimize the catalysts’ activity, while the controlling facet orientation in non-precious metals remains challenging. Here we show a substrate-induced growth strategy to synthesize copper nanosheets with specific (200) and (111) facet orientations over nitrogen/oxygen co-doped graphene (N/O-C) via chemical vapor deposition (Cu(200)@N/O-C and Cu(111)@N/O-C), revealing a distinct facet-dependent activity for ORR. It clarifies the substrate oxygen coordination promotes preferential Cu(200) growth under moderate deposition, whereas excess vapor drives thermodynamically favored Cu(111) formation. Theoretical and experimental analyses demonstrate Cu(200) facet in N/O-C optimizes the adsorption energies of ORR intermediates and lowers the reaction barrier for the rate-determining step to outperform the Cu(111), which also exhibits better activity compared to commercial Pt/C. When integrated into zinc-air batteries, Cu(200)@N/O-C delivers a power density of 222.5 mW·cm -2 and stable operation for over 1000 hours, highlighting the potential of facet-engineering on earth-abundant Cu for next-generation energy storage.
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Nature Protocols
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2026
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IF 18.4
Wang Keyun,Li Kejia,Yan Jiayang,Xue Lianji,Ma Yanni,Zhu Haiyang,Yu Ting,Wang Yan,Savitski Mikhail,Ye Mingliang
The peptide-centric local stability assay (PELSA) was developed to identify the protein targets and binding regions of diverse ligands at a proteome-wide scale. In this approach, the ligand is added to a cell lysate, and the proteins are digested for a short, defined time period using 0.5 mg ml −1 trypsin. Protein regions involved in ligand binding show less digestion, making it possible to identify binding sites and even quantify binding activity by dose-dependent analysis. In contrast to modification-based techniques that require chemical modification of ligands, the ligand modification-free property of the PELSA offers a key advantage by avoiding derivatization, thereby enabling proteome-wide identification of target proteins across diverse ligand types and biological contexts. The PELSA is broadly applicable for identifying protein targets of diverse ligands such as drugs and antibodies as well as endogenous metabolites and metal ions. However, performing PELSA experiments and subsequent data analysis to extract protein–ligand interaction details, including the binding protein, binding region and binding affinity, is not a trivial task. To address this, we present a detailed protocol for the experimental and computational PELSA workflow, including raw data processing and result visualization with the PELSA-Decipher software ( https://zenodo.org/records/18266507 ). Key points, such as selection of ligand concentrations, timing of trypsinization and quality control between different replicates, are discussed. Finally, we demonstrate the application of the protocol for identifying targets of staurosporine and 5-methyltetrahydrofolate, as well as for the dose-dependent PELSA analysis of inhibitors targeting the HSP90 family. The full PELSA protocol can be finished in 2 days, 1 day for sample preparation and 1 day for liquid chromatography–tandem mass spectrometry analysis and data processing. PELSA-Decipher can be downloaded from GitHub ( https://github.com/DICP-1809/ ).
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2026
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IF 4.7
Liu Y,Mao H,Tian Z,Wang X,Bao C,An B,Yang C.
The healing of the tendon-bone interface remains a significant challenge due to the lack of suitable bioactive materials for promoting interface regeneration. To address this, we developed a photosensitive hydrogel system designed to mimic the extracellular matrix. This system is based on light-induced imine crosslinking between type I collagen (Col I) and o-nitrobenzyl alcohol (NB)-modified hyaluronic acid (HANB). Experimental results demonstrate that the hydrogel (Col I-HANB) exhibits excellent controllability, low swelling rate, biodegradability, and tissue-adhesive properties. Using a fluorescent dye (8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt, HPTS) as a model molecule, we confirmed that the hydrogel enables localized and sustained release, high-lighting its potential as a drug-delivery platform for tissue engineering. To further enhance tendon-bone interface repair, the hydrogel was functionalized with two key components: (I) The E7 peptide, known for its stem cell-homing properties, was covalently immobilized into the hydrogel network via conjugation to bovine serum albumin (BSA) and followed by imine-ligation between UV-triggered aldehyde generation from HANB and BSA's amino groups; and (II) Kartogenin (KGN)-loaded nanoparticles (KGN-NPs) were incorporated to potentially promote chondrogenic differentiation, with release assays confirming sustained KGN delivery over 21 days. The composite hydrogel demonstrated excellent biocompatibility in cytotoxicity and live/dead assays. Importantly, in a rabbit rotator cuff tear model, it effectively promoted fibrocartilage regeneration and significantly improved tendon-bone healing, creating a favorable microenvironment for repair. The results above indicate that the hydrogel system provides a good microenvironment for rotator cuff injury repair and is a promising scaffold applied in treatment of rotator cuff injury.
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WATER AIR AND SOIL POLLUTION
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2026
·Vol. 237
·期 13
·779
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IF 3.5
Peng Ziling,Wang Xiu,Tan Fatang,Zhou Xian,Fan Zeyu,Lu Qi,Chen Xia,Shi Yu,Xia Yu
For the first time, a series of N-doped graphitic carbon-modified g-C 3 N 4 samples were synthesized via thermal polycondensation by incorporating trace amounts of chitosan into melamine precursors. The photocatalytic performance of the samples was evaluated through Rhodamine B (RhB) degradation under visible-light irradiation. The photocatalyst prepared with 50 mg chitosan achieved complete degradation of 10 mg/L RhB within 30 min, outperforming pristine g-C 3 N 4 . After five consecutive cycles, the photocatalyst maintained 91% degradation efficiency within 30 min while preserving its original crystalline structure, demonstrating outstanding stability and reusability. The large specific surface area, enhanced electron transfer efficiency, extended visible light absorption and low photogenerated electron–hole recombination rate were considered to contribute to the extraordinary visible-light-driven (VLD) photocatalytic activity of N-doped graphitic carbon-modified g-C 3 N 4 . Furthermore, a scavenger study was conducted to identify the dominant reactive oxygen species (ROS) involved in the photocatalytic degradation of RhB, providing deeper insights into the underlying reaction mechanisms.
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Nature Communications
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2026
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IF 18.1
Jin Huihui,Shen Zewen,Yan Chumin,Liu Yang,Tian Yingying,Bao Hongliang,Ji Zhuoyu,Hu Yezi,Zhao Guixia,Wang Xiangke,Huang Xiubing
Radioactive organic wastewater management concerning metal source recovery and organic matter degradation conforms well to the notion of circular economy and environmental protection, but the concurrent conversion in an integrated process is challenging and has been rarely studied. Here, we introduce a high-performance photoelectrochemical system for uranyl extraction and the simultaneous degradation of co-existing urea to manage the treatment of uranyl-containing radioactive organic wastewater generated from the production of nuclear fuel for fourth-generation high-temperature gas-cooled reactor. Cost-efficient self-standing Ni-modified TiO 2 nanotube arrays (Ni/TiO 2 ) and β-cyclodextrin polymer (P-CDP@CC) were used as photoanode and cathode, respectively. Concurrent photoanodic urea degradation and cathodic uranyl reduction towards UO 2 from low-level radioactive organic wastewater demonstrated a uranyl extraction efficiency of 99.08% and the urea degradation efficiency of nearly 100% under a current density of 4.5 mA/cm 2 within 8 hours in a single-compartment electrolysis cell. This work provides an effective, environmentally benign approach to upcycle uranium resources and purify organic pollutants from complicated wastewater matrix.
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