ACS Applied Nano Materials
·
2026
·
IF 5.8
Qi Ke,Ye Tian,Yuting Zeng,Zheng Liu,Zhijia Cai,Xilin Xiong,Pengyu Wu,Yuan Huang,Xianhui Liu,Suxiao Wang
Amyloid protein-induced endothelial leakage (APEL) may promote endothelial dysfunction and enhance Aβ oligomers aggregation, suggesting a potential mechanism in Alzheimer’s disease pathogenesis. Herein, we propose a therapeutic strategy to suppress the APEL effect. The approach involves engineering an Aβ-targeting peptide KD8 conjugated transferrin to selectively capture circulating Aβ oligomers in the bloodstream. Upon the transferrin-peptide-Aβ oligomers formation, the complex is immobilized by transferrin receptor-overexpressing MDA-MB-231 tumor cell membrane-derived nanovesicles through the transferrin receptor. The resulting ″Aβ oligomer-vesicle″ complex exhibits a substantially increased hydrodynamic diameter. This size amplification is hypothesized to mitigate the APEL effect by two potential mechanisms. First, the enlarged complex sizes may exhibit reduced permeability across the intact vascular endothelial barrier. Second, and more critically, the surface immobilization of Aβ oligomers likely imposes significant steric and geometric constraints, impairing their accessibility and binding kinetics to key endothelial targets, such as VE-cadherin. This interference would disrupt the oligomers’ capacity to compromise endothelial tight junctions and initiate leakage. Consequently, this approach aims to systemically reduce the risk of the APEL effect through combined physical sequestration and molecular interference. This mechanism not only prevents the onset of amyloid protein-induced endothelial leakage (APEL) and inhibits further fibril accumulation but also actively promotes the clearance of existing amyloid deposits, underscoring its therapeutic potential for Alzheimer’s disease.
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Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 6
·125412
·
IF 7.5
Huaqing Liu,Yujian Hu,Wenjuan Liu,Mengcheng Zhang,Wenrui Zhang,Yanlong Wang
A spatially regulated Fe⁰@SiO₂@nCaO₂ nanocomposite was rationally designed. • A multifunctional material integrates oxidation, precipitation, and flocculation. • Fe loading regulates ROS generation and pollutant-specific removal pathways. • Sequential activation of oxidation, precipitation, and flocculation pathways was revealed. The simultaneous removal of coexisting organic and inorganic contaminants remains a major challenge for wastewater treatment because conventional technologies fail to integrate oxidative degradation with inorganic immobilization in a single reaction system. Herein, a spatially regulated Fe 0 @SiO 2 @nCaO 2 multifunctional nanocomposite was developed to achieve synergistic purification of representative organic pollutants (sulfamethoxazole and atrazine) and inorganic contaminants (As(III) and phosphate). Batch experiments showed that the optimized Fe 0 @SiO 2 @nCaO 2 composite (Fe loading of 170.2 mg g −1 , 1 g L −1 ) removed 97.9% of sulfamethoxazole and 99.9% of atrazine, As(III), and phosphate within 24 h at an initial concentration of 20 mg L −1 for each contaminant. Radical–quenching experiments combined with electron paramagnetic resonance analysis revealed the involvement of 1 O 2 and •O 2 - , with a possible contribution from •OH. Notably, 1 O 2 showed the strongest apparent influence on sulfamethoxazole oxidation under the present alkaline conditions. Arsenic removal involved an initial As(III) oxidation step, followed by adsorption onto Fe-based sites and final immobilization through Fe/Ca–Si flocculation, rather than direct precipitation. Phosphate was primarily immobilized through rapid calcium-induced precipitation followed by incorporation into Fe/Ca–Si flocs. In the ternary sulfamethoxazole–As(III)–phosphate system, phosphate precipitation, organic oxidation, and arsenic immobilization exhibited distinct temporal separation. Matrix-effect experiments further showed that humic acid mainly inhibited As immobilization. Nevertheless, in a real-water matrix, 24-h removal efficiencies remained 92.1%, 99.5%, and 97.0% for SMX, phosphate, and As, respectively. These results demonstrated that Fe 0 @SiO 2 @nCaO 2 integrated complementary oxidation, precipitation, and immobilization pathways while maintaining effective performance under complex water-matrix conditions.
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JOURNAL OF NUCLEAR MATERIALS
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2026
·
IF 3.3
Qing Song,Wanjun Zhang,Qingguo Chen,Rui Ma,Wei Xu,Sultan Mehmood,Liang Bian,Xiaofeng Zhao
Precipitation enrichment coupled with ceramic solidification represents a technically viable and promising engineering approach for volume reduction and immobilization of high-level radioactive waste. Rhabdophane has been proposed as a candidate host phase for immobilizing actinides from high-level liquid waste (HLLW). However, the wet-chemical co-precipitation mechanism governing the simultaneous incorporation of tetravalent actinides and divalent charge-compensating cations remains incompletely understood. This knowledge gap is particularly relevant because Ba 2+ is a major product in HLLW generated from commercial nuclear power reactors. In this study, we investigate the Ba 2+ /Th 4+ co-incorporation behavior and solubility limits in La 1–2 x Th x Ba x PO 4 ·nH 2 O rhabdophane synthesized at pH=1 and 90 °C. Our results suggest that an excess of Ba 2+ is required to stabilize a single-phase rhabdophane, with an optimal Ba:Th ratio of 5. Combined structural, spectroscopic, and microscopic analyses confirm effective substitution of Ba 2+ and Th 4+ at La 3+ lattice sites for x ≤ 0.05. Higher doping levels lead to the crystallization of rhabdophane with nonstoichiometric compositions, accompanied by Ba 2+ vacancy defects. Over 99% of Th 4+ in 0.05 mol/L solution was removed within 12 h. Upon thermal treatment, the as-synthesized rhabdophane powders can be converted into dense monazite ceramics at 1250 °C.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Alexey Tarabarov,Xingxing Wang,Yige Lu,Jing Jiang,Yifei Cheng,Kexin Huang,Jiayao Yang,Zhuqian Xiao,Fangfang Fan,Dapeng Li,Hongpeng Wang,Jun Huang
Universal antibody purification remains challenging because conventional ligands (Protein A/G/L) target specific antibody regions, limiting their general application. In contrast, protein M from human mycoplasma exhibits unprecedented broad-spectrum binding to diverse antibody types through interaction with the conserved part of the variable domain of the light chain. Its engineered variant, protein M-8His, introduces pH-responsive histidine residues while maintaining this broad binding capability, but the molecular mechanisms governing its variable performance across different antibodies remain poorly characterized. To investigate this, we developed an antibody purification platform through site-specific immobilization of protein M-8His on Ni 2+ -modified superparamagnetic nanoparticles (NiNPs). NiNPs demonstrated a protein M-8His recoverable binding capacity of 74.41 mg/g. The resulting protein M-8His functionalized NiNPs (MNiNPs) demonstrated broad-spectrum antibody binding, with recoverable binding capacities up to 52.01 mg/g for trastuzumab scFv, while maintaining 76.1 % activity after 15 days of storage. Molecular dynamics simulations suggested two distinct interaction patterns: histidine-dominated interactions driving pH-dependent elution (H337/H338 for trastuzumab scFv) and mixed-interaction binding with reduced histidine contribution (for S2P6 and 76E1 antibodies), explaining the observed performance variations. Energy decomposition analysis showed that trastuzumab scFv (100 %) and 76E1 Fab (85.1 %) predominantly bind via Fv domain while S2P6 Fab mainly binds via constant domain (76.7 %). These findings provide mechanistic insights into protein M-8His interactions and lay a foundation for optimizing protein M-8his for industrial applications.
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POLYMER
·
2025
·
IF 4.5
Guangshui Tu,Chong Wang,Shengyou Chen,Qian Wang,Zhenxue Liu,Haotian Zhou,Chunyu Feng,Handou Zheng,Haiyang Gao
Heterogeneous catalysts have long dominated polyolefin production, the immobilization of a pyridylamido hafnium catalyst is highly challenging because of its unique in-situ ligand modification mechanism. Here, we report a heterogeneous pyridylamido hafnium catalyst immobilized on a dual MgCl 2 /Al i Bu n (2-ethyl-1-hexoxide) 3-n activator/support for slurry homo- and copolymerization of 4-methyl-1-pentene (4MP). The supported pyridylamido hafnium catalyst shows enhanced catalytic activity and excellent polymer morphology control toward homopolymerization of 4-methyl-1-pentene. The produced poly(4-methyl-1-pentene)s show high isotacticity (>99%) and high melting temperatures up to 230 °C, and have normal molecular weight distribution. The heterogeneous catalyst still remains the advantages of enhanced catalytic activity and excellent polymer morphology control during the copolymerization of 4MP and α-olefins. Both the incorporation of α-olefin and longer chain α-olefins can improve the resilience of PMP materials.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 18
·4565
·
IF 4.183
Shangyong Li,Yanan Wang,Xiao Li,Beom Suk Lee,Samil Jung,Myeong-Sok Lee
Bacterial biofilm causes severe antibiotic resistance. An extracellular polymeric substance (EPS) is the main component in the bacterial biofilm. Alginate is a key EPS component in the biofilm ofPseudomonas aeruginosaand responsible for surface adhesion and stabilization of biofilm. Alginate lyase has emerged as an efficient therapeutic strategy targeting to degrade the alginate in the biofilm ofP. aeruginosa. However, the application of this enzyme is limited by its poor stability. In this study, chitosan nanoparticles (CS-NPs) were synthesized using low molecular weight chitosan and alginate lyase Aly08 was immobilized on low molecular weight chitosan nanoparticles (AL-LMW-CS-NPs). As a result, the immobilization significantly enhanced the thermal stability and reusability of Aly08. In addition, compared with free Aly08, the immobilized AL-LMW-CS-NPs exhibited higher efficiency in inhibiting biofilm formation and interrupting the established mature biofilm ofP. aeruginosa, which could reduce its biomass and thickness confirmed by confocal microscopy. Moreover, the biofilm disruption greatly increased the antibiotic sensitivity ofP. aeruginosa. This research will contribute to the further development of alginate lyase as an anti-biofilm agent.Keywords:immobilization;chitosan nanoparticles;alginate lyase;anti-biofilm activity;antibiotics susceptibility
研究领域:
微生物学
·
生物技术
·
医学
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Bioresources and Bioprocessing
·
2018
·Vol. 5
·期 1
·1-8
·
IF 0
Gao Zhen,Yi Yunfei,Zhao Jia,Xia Yongyang,Jiang Min,Cao Fei,Zhou Hua,Wei Ping,Jia Honghua,Yong Xiaoyu
Background Laccase, a multicopper oxidase that catalyzes the oxidation of phenols, aromatic amines, and benzenethiols, has attracted much attention in applications of organic synthesis, bioremediation, and pulp/textile bleaching. However, free laccases cannot be recycled and are easily inactivated in diverse environmental conditions. Enzyme immobilization is a promising strategy to improve stability, resistance to extreme conditions, and reusability of laccase. Results In this study, amino-functionalized magnetic Fe 3 O 4 nanoparticles were synthesized for co-immobilization of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) and laccase by glutaraldehyde cross-linking method. The magnetic nanoparticles were characterized with FTIR, XRD and VSM. Cyclic voltammetry was carried out to verify electrochemical behaviors of the co-immobilized laccase and TEMPO nanoparticles. When the co-immobilized laccase and TEMPO nanoparticles were used to decolorize acid fuchsin, the maximum decolorization rate of 77.41% was obtained with the ratio of TEMPO to laccase being 0.3 mM/g:120 U/g. Conclusion The co-immobilized nanoparticles retained above 50% residual activity after eight cycles of operation, which presented an approach to develop a co-immobilized laccase and mediator system for potential industrial application.
研究领域:
生物化学
·
环境科学
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Ganning Zeng,Hanzhang You,Mingming Du,Yan Zhang,Yimei Ding,Chengda Xu,Bo Liu,Bing Chen,Xiangliang Pan
Remediating and controlling PAHs (Polyaromatic Hydrocarbons) contamination in the environment is essential all over the world. Nano-TiO 2 immobilized on large surface area adsorbent is a kind of emerging endeavor to enhance the photo-catalysis performance. In this paper, activated carbon samples (ACs) were synthesized by using a kind of subtropical macroalgae, i.e., Sargassum Horneri ( S.H. ) as raw material. ACs activated by FeCl 3 , ZnCl 2 , a composite activators mixture of FeCl 3 and ZnCl 2 (CA) were selected to immobilize nano-TiO 2 in aquatic environment, and naphthalene degradation performance of these materials was systematically assessed. The results showed that ACs could provide more activated space for naphthalene photocatalytic reaction, in which Zn 2+ helped to hold relative high specific surface area, Fe 3+ helped to extend the valid light responsive wavelength, and CA could take advantage of both metal ions, with the largest whole volume and surface functional groups. The highest naphthalene degradation efficiency of TiO 2 /AC-CA under simulated sunlight could arrive to 77.5%, while those of TiO 2 /AC-FeCl 3 and TiO 2 /AC-ZnCl 2 were 65.0%, 54.5%, respectively. Meanwhile, the pH-resistance and reusing capability of TiO 2 /AC-CA are better than those of other TiO 2 /ACs. The results helped to clarify the synergistic mechanism of AC and TiO 2 in photo-catalysis, especially the metal ions’ role in combining the two materials, and showed the industrial potential of S.H. in PAHs contamination.
研究领域:
环境科学
·
材料科学
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LANGMUIR
·
2026
·
IF 4.4
Zhihai Wu,Gongxuan Lu
The highly efficient enrichment of Li+ and Cs+ from their low-concentration aqueous solutions is a crucial step for Lithium and Cesium clean production, as well as radioactive Cs+ safety remediation from water. The current adsorption and precipitation routes are high energy cost and environmental unfriendly. Electrochemical ion exchange route may serve as a promising alternative route. This study presents results of high efficient, multicycle Li+ and Cs+ enrichment from their low concentration solutions via intercalation and deintercalation in WO3/GaAs composite electrode with Faraday efficiency above 99% and high intercalation capacities 7.25 mg/g for Li+ and 38.89 mg/g for Cs+, respectively. After six cycles, 95 and 90% capacities for Li+ and Cs+ still remained. Both enrichment efficiency and stability are higher than those of LiFePO4 (LFP) and LiMn2O4 (LMO) under the same conditions. This route might provide a way for low-energy cost and highly efficient Li+ and Cs+ recovery from contaminated water.
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高影响因子
Plants-Basel
·
2026
·Vol. 15
·期 3
·483
·
IF 4.7
Huagang Huang,Jinsong He,Denglu Liu,Haiying Yu,Lu Zhang,Tao Liu
Tobacco (Nicotiana tabacumL.) has a propensity to accumulate cadmium (Cd), especially in its leaves, which can have a detrimental impact on yield, quality, and product safety. The development of low-accumulation cultivars is a vital mitigation approach; however, the underlying mechanisms remain inadequately understood. In this study, through pot experiments, the physiological mechanisms responsible for the differential Cd accumulation between the low-accumulating tobacco line CF986 and the high-accumulating Yuyan5 were explored. A comprehensive analysis was conducted on the organ-specific Cd distribution, chemical speciation, subcellular compartmentalization, and photosynthetic responses across a gradient of Cd exposure. In comparison with Yuyan5, CF986 accumulated significantly higher amounts of Cd in the roots and stems, but substantially lower amounts in the leaves. Specifically, the Cd content in the leaves of CF986 was only 64.32–68.74% of that in Yuyan5 across different Cd exposure levels. The organ-specific Cd distribution pattern in CF986 followed the order: leaf > stem > root. Moreover, the proportion of Cd partitioned to the leaves was lower in CF986 compared to Yuyan5, while the roots and stems exhibited enhanced Cd retention, with Cd levels in stems reaching up to 2.04 times higher than those in Yuyan5. Analysis of the chemical forms and subcellular distribution of Cd indicated that the mobile Cd fractions in the stems of CF986 were significantly reduced compared to Yuyan5. A larger proportion of Cd was immobilized in the stem cell-wall fraction, which enhanced Cd retention and restricted xylem-mediated transport to the leaves. Cd exposure did not significantly affect the concentration of foliar photosynthetic pigments in CF986; however, it notably inhibited the activity of the photosystem II (PSII) reaction center. At higher Cd levels, the photoprotective thermal dissipation gradually failed, with a decrease of up to 41.36% in ΦNOfor CF986 compared to CK under Cd4.0 treatment. This research unveiled a stem barrier mechanism, whereby Cd translocation to the leaves is restricted through chemical and subcellular sequestration in the stem. This mechanism provides a novel perspective on both plant heavy metal allocation and the assurance of crop safety.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yan Cai,Weifu Ren,Haiyan Wang,Qi Bian
Diabetic kidney disease (DKD) is a common and serious kidney-related complication of diabetic mellitus. Although albuminuria and estimated glomerular filtration rate (eGFR) are commonly used diagnostic biomarkers, they have limitations in the diagnosis of DKD due to the lack of specificity and sensitivity. Urinary N-glycans are emerging as novel biomarkers for predicting renal prognosis in DKD. However, most of the current N-glycan profiling methods for DKD were based on lectin affinity enrichment or hydrophilic interaction chromatography (HILIC) with optical detection, which provided limited N-glycome coverage in low throughput. Herein, we developed a novel N-glycan purification method, termed Ultrafast Glycoprotein Immobilization for Glycan extraction (UltraGIG), for in-depth profiling of urinary N-glycome in DKD using mass spectrometry (MS). In UltraGIG, proteins were rapidly (within 40 min) immobilized to resin with high efficiency (over 98%) through NHS-reactive chemistry , and then N-glycans were enzymatically released from the resin by PNGase F. Owing to good efficiency of immobilization of proteins to resin, the subsequent washing steps to remove proteins and other impurities could be easily performed. The UltraGIG showed good selectivity towards glycans (extracting N-glycans from the mixture of IgG and BSA at a 1:100 M ratio) and samples loss was minimized (detection sensitivity at fmol level). A total of 237 N-glycan compositions were identified from urine samples with DKD and healthy controls, which representing the largest data set of N-glycome from DKD. Compared to healthy controls, 3 N-glycans were up-regulated and 14 N-glycans were down-regulated in DKD patients. Collectively, UltraGIG offers a competitive sample purification method for in-depth analysis of urinary N-glycome by MS and provide new insights into biomarker study for the diagnosis of DKD.
研究领域:
医学
·
生物化学
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高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2021
·Vol. 39
·期 10
·2889-2897
·
IF 6
Cuiyuan Liang,Xin Wang,Dexin Yu,Wei Guo,Feng Zhang,Fengyu Qu
Main observation and conclusion Detection of hydrazine originated from electrochemical media has recently gained considerable attention in the sensing field. Herein, to improve electron transfer capacity, a polyoxometalate metal-organic framework (POMOF, NENU-3) is in-situ nucleated onto the carboxyl functionalized reduced graphene oxide (CFG) (NENU-3/CFG, abbreviation N3/CFG). The N3/CFG supported onto carbon cloth electrodes (CCEs) has been investigated for hydrazine detection. The amperometric results display that the POMOF/CFG to hydrazine has a broad linear range (0.09—362.5 μmol/L) and low detection limit (24 nmol/L). In addition, the POMOF/CFG-based sensors possess good anti-interference capability, boosted stability and feasibility. Furthermore, when applied to the detection of practical samples, acceptable relative recoveries of 96.26%—107.30% are obtained.
研究领域:
材料科学
·
分析化学
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高影响因子
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