International Journal of Medical Sciences
·
2020
·
IF 2.523
Hao Hu,Liang Xu,Shao-Ju Luo,Ting Xiang,Yan Chen,Zhi-rui Cao,Yu-jian Zhang,Zhuomao Mo,Yongdan Wang,Dong-fang Meng,Ling Yu,Li-zhu Lin,Shi-Jun Zhang
Retinal dehydrogenase 5 (RDH5) is an important enzyme in the visual cycle. Several studies have reported that the RDH family may play crucial roles in tumor prognosis. However, the role of RDH5 in tumor prognosis is still unclear. We examined the mRNA level of RDH5 by using q-PCR in hepatocellular carcinoma (HCC) and adjacent non-cancerous tissues. The proliferation rate of HCC cells was detected by MTS assay, and the invasive ability was examined by transwell and scratch wound assays. The YAP protein localization and expression were visualized by immunofluorescence in two different cell lines. CpG islands in the promoter region were predicted by using the methprimer database. Clinical characteristics of a patient cohort data came from The Cancer Genome Atlas database. RDH5 was significantly downregulated in hepatocellular carcinoma tissues, and low RDH5 expression was associated with metastasis and poor patient prognosis. Functional assays revealed that the RDH5 promoter is methylated in HCC cell lines. Moreover, overexpressing RDH5 can suppress metastasis by reversing the epithelial-mesenchymal transition (EMT) process, and RDH5 also inhibits cell proliferation in HCC cell lines. Furthermore, suppressing RDH5 can activate the Hippo/YAP signaling pathway and promote the nuclear translocation of YAP. Clinical data demonstrated that RDH5 is an independent prognostic factor in HCC. In our study, we provided the first evidence that RDH5 plays a crucial role in suppressing proliferation and metastasis, and the RDH5 promoter is methylated in hepatocellular carcinoma. And as an important regulator, RDH5 can suppress the Hippo/YAP signaling pathway. Taken together, it revealed that RDH5 might be a potential therapeutic target in HCC patients.
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PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2021
·Vol. 40
·期 2
·247-255
·
IF 6
Qian Wang,Huanshun Yin,Yunlei Zhou,Lulu Cao,Zhengkun Yu,Yamin Xu,Shiyun Ai
Comprehensive Summary As a key epigenetic modification, 5-formylcytosine (5fC) controls many important cell functions, such as gene expression and cell differentiation. However, 5fC exists in mammalian tissues and cells with low content, and its structure is highly similar to 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-carboxycytosine (5caC), which requires the detection method for detecting 5fC with high sensitivity and specificity. In this experiment, WS 2 /Bi/Bi 2 O 2 CO 3 was used as the photoactive material, 5fC antibody was used as the target molecule recognition reagent, and rolling amplification (RCA) was employed as the signal amplification strategy to construct a novel photoelectrochemical biosensor for detecting 5-formylcytosine deoxynucleotide (5fdCTP). Firstly, WS 2 /Bi/Bi 2 O 2 CO 3 nanocomposite was prepared and characterized, and then modified on the ITO electrode. Then, polyacrylic acid (PAA) was immobilized by electrostatic adsorption, which was employed as the antibody capture reagent based on the covalent reaction of –COOH on PAA with –NH 2 on Ab. Subsequently, 5fdCTP was recognized through immunoreaction, triggering the subsequent rolling circle amplification reaction with the aid of the crosslinker of Zr 4+ . Finally, positive methylene blue (MB) was adsorbed by negative ssDNA, resulting in an increased photoelectric conversion efficiency of WS 2 /Bi/Bi 2 O 2 CO 3 and improved detection sensitivity. The linear range of the PEC biosensor for detecting 5fdCTP was 0.05—100 nmol/L, and the minimum detection concentration was 12.4 pmol/L (S/N = 3). The applicability of this method has been evaluated by studying the influence of Cd 2+ and antibiotic concentration on the 5fC content in the genomic DNA of maize seedlings.
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ACS Catalysis
·
2025
·
IF 13.1
Wang Xu,Yuhuang Huang,Zhuohan Lin,Qiaohui Ruan,PeiQing Yuan,Yan Li
Efficient zeolitic recycling of polyolefin waste into C2–C4 light olefins offers an attractive route toward resource recovery; however, conventional zeolite catalysts suffer from severe mass transport limitations that lead to undesired secondary reactions and coke formation. Here, we report a “structure modulation and intelligent optimization” strategy to develop hierarchical ZSM-5 (H-ZSM-5), integrating tailored mesoporosity and crystal size control via Bayesian optimization (BO). Catalytic performance was evaluated in a two-stage system combining thermal pyrolysis and subsequent catalytic cracking. BO efficiently navigates this multivariate design space, identifying an optimal architecture (∼100 nm crystals, 4.0 nm mesopores) that achieved 92.6 wt % total gas yield and 85.1 wt % light olefin selectivity at 500 °C with a low catalyst-to-feed ratio (0.2). The nanoscale H-ZSM-5 also exhibited durability over 40 consecutive cycles and broad compatibility with postconsumer plastic mixtures. Mechanistic studies revealed that the synergy between nanoscale morphology and hierarchical porosity enhances mass transport and acid site accessibility, demonstrating that complex catalyst architectures can be precisely optimized via artificial intelligence strategies, such as BO.
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DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Lifang Cao,Pu Wang,Yuhan Tang,Hong Liu,Jiasheng Wang
Amorphous ZSM-5 was facilely synthesized by simply tuning the aqua ammonia amount and used as support of Mo-V-O for aerobic oxidation desulfurization. It was found that both geometric and electronic effects help to enhance the catalytic performance. Geometrically, the amorphous ZSM-5 could better disperse the metals due to its higher specific area. Electronically, the amorphous ZSM-5 could enable V to enrich electrons availing for oxygen activation to superoxide radical thanks to its low work function. The superoxide radical combines with Mo to form peroxide and then oxidize the sulfur-containing substrates. 100% desulfurization can be realized at 80°C for model oil with sulfur content of 500 ppm over the amorphous ZSM-5 supported Mo-V-O catalyst. This study provides inspiration for designing efficient catalysts for aerobic oxidation desulfurization.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Hua Yang,Leqiong Jin,Dengqi Zhao,Zhifeng Lian,Manikandan Appu,Jianying Huang,Zibin Zhang
An omnipresent pathogenic bacterium, Pseudomonas aeruginosa (P. aeruginosa PAO1), is easy to contaminate environmental water or foods, causing daily food spoilage and infections. The biofilm-forming ability and bacterial resistance of P. aeruginosa PAO1 make it difficult to be eradicated by traditional bacteriostatic agents. In this work, we designed and synthesized a pyridinium-based pillar[5]arene (PP5), while trimethylammonium-based pillar[5]arene (TP5) was used as a control compound. After clear characterization, the antibacterial and antibiofilm activities as well as the microbial resistance of TP5 and PP5 against P. aeruginosa PAO1 were extensively examined. It was revealed that PP5 exhibited good inhibition activity with the minimum inhibitory concentration (MIC) of 0.051 mmol/L, while no significant antibacterial and biofilm formation activity for TP5 against P. aeruginosa PAO1 was observed. More importantly, PP5 had negligible antimicrobial resistance even after 18th passages. A transmission electron microscope (TEM) showed that PP5 could physically disrupt the cell membranes, causing the leakage of internal constituents, which is possibly ascribed to the synergistic penetrability and π–π interactions of strain, thus greatly reduced the development of bacterial resistance. Overall, the presented studies indicated that pyridinium moieties could facilitate the cationic pillar[5]arene to generate surprising antibacterial and antibiofilm formation ability against P. aeruginosa PAO1.
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高影响因子
TRANSITION METAL CHEMISTRY
·
2021
·Vol. 47
·期 1
·11-18
·
IF 1.588
He Xiong,Chen Fangmin,Zhou Chichi,Zhang Dan,Li Yan,Zhang Xiuqing,Liu Hanfu
Based on 5-nitroisophthalic acid (5-H 2 nip) and bis(4-pyridyl)amine (dpa), the complexes {[Cd 6 (5-nip) 6 (dpa) 4 ]·EtOH} n ( 1 ) and [Co 5 (5-nip) 2 (dpa) 2 (OH) 2 (SO 4 ) 2 (H 2 O) 2 ] n ( 2 ) have been synthesized and characterized by elemental analysis, single-crystal X-ray diffraction, IR spectroscopy, thermal gravimetry analysis, fluorescence and magnetic susceptibility. The carboxylate groups of the 5-nip 2− adopt three different bridging modes [ bis (chelate-bidentate), bis (bidentate) and bis (monodentate)]. The 5-nip 2− anions and dpa play as linkers, which connect Cd(II) or Co(II) ions to form the three-dimensional structures of 1 and 2 . Moreover, 1 exhibits intense solid-state luminescence emissions centered at 402 nm at room temperature, which mainly originates from the intraligand π – π* transitions of 5-nip 2− . Magnetic susceptibility measurement indicates that 2 shows antiferromagnetic interactions between the Co(II) ions.
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高影响因子
NANOTECHNOLOGY
·
2025
·Vol. 36
·期 17
·175701
·
IF 2.8
Wenjuan Zhang,Kangning Xiong,Dashuai Yan
The wear and corrosion resistance of water-based polytetrafluoroethylene (PTFE) coating need to be further improved to cope with various harsh environmental conditions. In this paper, a mulberry-like ZSM-5 molecular sieve was prepared and then was modified to enhance the uniformity, wear resistance and hardness of PTFE coating. In addition, a ZSM-5/MoS2/PTFE coating was developed by adding MoS2 in the chemical composition of coatings, which significantly increased the critical cracking thickness of PTFE to 27 μm. The wear amount of ZSM-5/MoS2/PTFE coating is only 5.7 mg, and after 680 h of salt spray, there is no obvious corrosion on the surface. The friction coefficients under applied loads of 5 N, 10 N, and 20 N are 0.007, 0.057, and 0.055, respectively. The experiment results show that the coating possesses excellent lubrication, wear resistance, and corrosion resistance, and its performance and cost are far superior to commercial products.
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PubMed
高影响因子
American Journal of Cancer Research
·
2022
·
IF 5.942
Yuxin Zhuang,Guang Yang,Shaoyu Wu,Jianjun Chen,Jiayin Guo,Dongling Quan,Tingting Zhang,Zichao Yang,Shaobin Tan,Yuheng Ji,Zhipeng Chen,Lin Lv
The composition of microtubules involving several steps, including the polymerization and depolymerization of α-tubulin and β-tubulin heterodimers. Microtubule-targeting agents can increase or inhibit microtubule polymerization, thereby disrupting the dynamic process and stalling cells in G2/M phase. Microtubule-targeting agents are generally cytotoxic, which neurological toxicity being one of the significant adverse events associated. We recently reported a novel 5-arylalkynyl-2-benzoyl thiophene (PST-3) that exhibited broad-spectrum cellular cytotoxicity and in vivo potency with high safety. PST-3 was a substrate of p-gp, which could not cross the blood-brain barrier and lead to less neurotoxicity. The antitumor activities in vitro demonstrated that PST-3 combined with the colchicine-binding site on microtubule, induces morphological changes, disrupts microtubule networks, inhibits polymerization of tubulin, arrests breast cancer cells in the G2/M phase of the cell cycle and induces apoptosis. Evaluation of the antitumor effect in vivo demonstrated that PST-3 elicited MDA-MB-468 tumor %T/C of 11.75%, whereas elicited MCF7 tumor %T/C of 44.38% in breast cancer xenograft models. Besides, in vivo experiments of a higher dose (60 mg/kg) of PST-3 treatment for 21 days did not produce any significant neurotoxicity. These results provide evidence that PST-3 might possess the potential to be developed into a new microtubule inhibitor without neurological toxicity.
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高影响因子
CytoJournal
·
2024
·
IF 2.5
Xinghua Liang,Xuelian Liu,Long Zhang,Junhao Liu,Rong Yan,Haiyan Li,Xiancheng Zeng,Hong Wang
Objective:
Colorectal cancer is severely challenging because of the insufficient understanding of the mechanism underlying its resistance to clinical chemotherapy. The purpose of our study is to investigate the role of the LIM protein Ajuba (JUB) in the chemoresistance of colon cancer and its potential effect on clinical treatment.
Material and Methods:
The protein levels of JUB in colon cancer tissues were evaluated using Western blot analysis and immunohistochemistry assays. The correlation between JUB and the prognosis of patients with colorectal cancer was determined using Kaplan–Meier plot analysis. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were employed to determine the 50% inhibitory concentration of 5-fluorouracil (5-FU) and thus assess the effect of JUB on the effectiveness of 5-FU. In addition, the rate of cellular apoptosis was measured using fluorescence-activated cell sorting assays. Side population and sphere formation analyses were conducted to determine the role of JUB in promoting the stem cell-like traits of colon cancer cells. In vivo assays were performed and detect whether the downregulation of JUB induces 5-FU sensitivity. Moreover, luciferase and Western blot assays were employed to uncover the mechanism through which JUB promotes chemoresistance in colon cancer.
Results:
JUB expression was upregulated in chemoresistant colon cancer (P < 0.001) and correlated with relapse-free survival (P = 0.000002). Functionally, the upregulation of JUB conferred 5-FU resistance to colon cancer cells in vitro, whereas the downregulation of JUB induced 5-FU sensitivity in colon cancer cells in vivo. The high expression of JUB promoted the tumorigenic capability of colon cancer cells. Furthermore, the increased expression of JUB activated multiple downstream genes of the Notch signaling pathway with increased expression in JUB-overexpressing cells but reduced expression in JUB-silenced cells. Importantly, the inhibition of Notch signaling using a small-molecule inhibitor significantly suppressed JUB-induced chemoresistance.
Conclusion:
Results suggest that JUB plays an important role and may serve as a biomarker for the clinical treatment of patients with 5-FU-resistant colon cancer.
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高影响因子
ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Caijin Zhou,Chun Shen,Kaiyue Ji,Jiabin Yin,Le Du
Aimed at efficient production of 5-hydroxymethylfurfural (HMF) in a green and sustainable way, dehydrogenation of fructose was enhanced by liquid–liquid extraction in a membrane dispersion microreactor. On account of the high mass-transfer rate resulted from dripping flow, the obtained HMF was readily extracted from the aqueous phase to the organic phase, effectively preventing the sequence side reaction and leading to high HMF selectivity. Enhanced by efficient extraction, the reaction duration decreased from 60 min in a traditional stirred reactor to 4 min in the microreactor, leading to an increase in the space-time yield by 3 orders of magnitude. The effects of total volume flow rate, droplet size, and phase ratio relating to extraction efficiency and HMF yield were systematically investigated. The highest extraction efficiency of nearly 100% coupled with the HMF yield of 93.0% was achieved at the phase ratio of 2 with volume flow rate of 600 mL/h. Overall, this work not only delineates an efficient strategy for synthesizing HMF but also opens a new avenue for reaction systems with subsequent side reaction, which suffer from low selectivity of the intermediates due to the in-line separation bottleneck under conditions of limited mass transfer.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 9
·6664-6672
·
IF 3.201
Yao-Yi Xu,Xue-Ling Wei,Shuai Liang,Ya-Li Sun,Zi-Sheng Chao
Considering the reliable characteristics and the suitable silica alumina content of kaolin, a new way of synthesizing a ZSM-5/NaA hybrid zeolite membrane on 170 mm length ceramic tubes by employing kaolin as a modification layer using a hydrothermal synthesis process was presented for the first time in this work. Kaolin was filtrated on the surface of the support (not only the ceramic tube, but also the NaA membrane layer) as a modification layer not only promoting the interface interlinkage among the support, NaA membrane and ZSM-5 membrane layer, but also promoting the growth of zeolite membranes. The SEM and XRD results showed that compact and homogeneous NaA and ZSM-5 zeolite layers were both formed with the thicknesses being 7.06 μm and 6.14 μm, respectively, after modification with kaolin. The permeation separation experiments demonstrated that the performance of the ZSM-5/NaA hybrid membrane was better than that of the monolayer zeolite membrane with permselectivities of H2 over CO2, N2, CO and CH4 of 5.40, 4.65, 4.70 and 3.93, respectively, which were higher than the corresponding Knudsen diffusion coefficients. Above all, modifying the support with kaolin and then synthesizing the ZSM-5/NaA hybrid membrane could strengthen the performance of the zeolite membrane effectively, which provided a new approach for the synthesis of high performance zeolite membranes in the field of membrane separation.
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高影响因子
NANO
·
2024
·
IF 1
Xuecong Ma,Linlin Geng,Yu Li,Jianwen Wei,Lei Liao,Dunqiu Wang
Micro/mesoporous materials are low-cost functional materials with the potential for CO 2 adsorption. Modifying micro/mesoporous materials is significant for enhancing CO 2 adsorption and their high-value utilization. Herein, the micro/mesoporous material of composite molecular sieve ZSM-5/MCM-48 (ZM) was synthesized by a two-step crystallization method using cetyltrimethylammonium bromide as a template, tetraethyl orthosilicate as a silicon source and ZSM-5 seed as part of silicon aluminum source. Tetraethylenepentamine (TEPA) and polyethyleneimine (PEI) were used as impregnation modifiers to modify ZM with amine groups. The results showed that with the increase of amine loadings, both ZM-T60 (Impregnated with TEPA) and ZM-P60 (Impregnated with PEI) functionalized adsorbents exhibited the best adsorption capacities of 4 . 9 7 mmol ⋅ g − 1 and 3 . 2 8 mmol ⋅ g − 1 at 60, respectively, when the loading of ZM sample was 60%. The adsorption kinetic analysis showed that the adsorption of CO 2 by ZM-T60 and ZM-P60 was a chemisorption-dominated process accompanied by physical adsorption. After 10 adsorption/desorption cycles, the CO 2 adsorption capacity of ZM-T60 and ZM-P60 decreased by 8.7% and 2.6%, respectively. This work demonstrates that impregnation modification of the composite molecular sieve to achieve efficient CO 2 adsorption has practical application significance.
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高影响因子
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