MOLECULAR NUTRITION & FOOD RESEARCH
·
2025
·
IF 4.2
Xin Li,Bohan Sun,Yanting Qin,Fangfang Yue,Xin Lü
Fecal microbiota transplantation (FMT) could significantly alter the recipient's gut bacteria composition and attenuate obesity and obesity-related metabolic syndromes. DL-norvaline is a nonproteinogenic amino acid and possesses anti-obesity potential. However, the specific mechanisms by which gut microbiota might mediate beneficial effects of DL-norvaline have not been completely elucidated. In this study, DL-norvaline-mediated FMT upregulated the beneficial bacteria ( Clostridia_UCG_014 , Christensenellales , Bacilli , Ileibacterium , Dubosiella , Lactobacillus , Muribaculaceae , and Bacteroidaceae ) and downregulated the harmful bacteria ( Tuzzerella and Marinifilaceae ), further intestinal inflammation, oxidative stress, and intestinal barrier were alleviated as well as short chain fatty acids levels were increased, thus alleviating glucose and insulin metabolism, improving biochemical indexes and energy metabolism and decreasing body weight gain and tissue weight. However, heat-inactivated FMT did not demonstrate any of those improvements in obese mice. Notably, both DL-norvaline-mediated FMT and heat-inactivated FMT increased Bacteroidaceae and Muribaculaceae , this being a signature of alterations to the gut microbiota marker caused by DL-norvaline. Therefore, the beneficial effects of DL-norvaline were transmissible via FMT. This study highlighted the pivotal involvement of the gut microbiota in the development of obesity and provided a novel insight into the underlying mechanisms of FMT, thereby potentially enhancing the efficacy and refinement of FMT utilization.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 12
·1-12
·
IF 5.3
Wang Yisi,Zhang Junying,Lv Xinxin,Ding Ya,Wang Yaolong,Liu Yuanhua,Wu Chunyong,Yang Gongjun
A highly sensitive and selective label-free impedimetric immunosensor based on AuNPs@pDA@NiCo 2 S 4 @MoS 2 nanocomposite modified on the surface of a screen-printed electrode (SPE) was designed for the detection of interleukin-6 (IL-6). The distribution of NiCo 2 S 4 nanoparticles on MoS 2 nanosheets was able to prevent them from agglomerating. The polydopamine (pDA) layer was coated on the surface of NiCo 2 S 4 @MoS 2 nanosheets by self-polymerization, which improved the stability and biocompatibility of the nanomaterial. The excellent reduction ability of pDA promoted the synthesis of gold nanoparticles (AuNPs), which increased the amount of antibody adsorption and the conductivity of the material. Finally, the antibody (Ab) of IL-6 was immobilized on the surface of AuNPs@pDA@NiCo 2 S 4 @MoS 2 nanocomposite. Electrochemical impedance spectroscopy (EIS) was used to detect the change of impedance before and after the immune response between Ab and IL-6 antigen (IL-6). Under the optimal experimental conditions, the relative change in impedance and the logarithmic concentration of IL-6 showed a good linear relationship in the range 1.00 to 1.00 × 10 6 pg/mL, with a low detection limit of 0.97 pg/mL. In addition, the proposed immunosensor performed with good reproducibility, stability, and specificity. It was successfully applied to the determination of IL-6 in patient’s serum samples of head and neck carcinoma with recoveries of 98.40% to 106.5%. To sum up, the proposed label-free impedimetric immunosensor was successfully constructed for IL-6 detection in real samples. Graphical abstract
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JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Qiqi Wang,Mei Liu,Shuo Wang,Lulu Shi
A novel supramolecular framework [Cd(QDS) 2 ]·(CB[6]) 2 ( 1 ) based on cucurbit[6]uril (CB[6]) and 8-hydroxyquinoline-5-sulfonic acid ion (QDS − ) was successfully synthesized under solvothermal conditions. The single crystal X-ray diffraction analyses indicated that the construction of this supramolecular framework is attributed to the non-covalent interactions between the QDS − and the outer surface of CB[6]. The performance study demonstrates that 1 exhibits a remarkable sensitivity and selectivity in detecting anions Cr 2 O 7 2− , MnO 4 − , furan antibiotics nitrofurantoin (NFT) and nitrofurazone (NFZ) through fluorescence quenching with the limit of detection (LOD) were 95, 89, 132, and 113 ppb, respectively. Meanwhile, due to the remarkable fluorescent properties of 1 , anti-counterfeiting marks were incorporated into the positioning boxes of quick response (QR) codes. The anticounterfeiting signs appear yellow in daylight while exhibit bright green fluorescence when excited at 365 nm. Furthermore, 1 also have been successfully applied to real samples with satisfactory results. These remarkable findings suggest that 1 hold promise as an effective fluorescent sensor.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yuanyan Luo,Tao Zhou,Costas Molochas,Yongkang Tan,Xiaoting Huang,Zhenyu Chen,Jinliang Zhu,Panagiotis Tsiakaras,Peikang Shen
Pt-Pd-Ni polyhedral nanobelt (PNBs) composite catalysts are synthesized in presence of tungsten hexacarbonyl [W(CO) 6 ] by a simple solvothermal method, combining both higher specific surface area and more index active site than the corresponding single-structure catalysts. The thermal decomposition of W(CO) 6 plays a crucial role in the morphology formation. The tenuous composite with a moderate nanobelt width of 7.46 nm formed after heating for 6 h (Pt-Pd-Ni PNBs2) shows better ORR performance and durability than the ternary (Pt-Pd-Ni) and binary (Pt-Pd) single structures. It exhibits a mass activity of 1.49 (A mg Pt −1 ), which only degrades by about 10.63 % after 50,000 durability cycles. The metal regulatory effects induced by the carbonyl compounds cause the contraction of the Pt-Pt bonds. Density functional theory calculations indicate that the compressive strain of Pt-Pd-Ni PNBs2 leads to a decrease in the d-band center and overpotential negatively shifted, thus provide a more sufficient and faster ORR proceeding process in thermodynamics, facilitating a higher energy conversion efficiency.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Zhang Shirong,Tan Yuxin,Liu Yilei,Li Yafei,Li Jishun,Zhang Shenglan,Pan Hongcheng
Quantum dots (QDs) have emerged as highly sensitive fluorescent markers for lateral flow immunoassays (LFIAs) due to their excellent fluorescence brightness and photostability. The incorporation of multiple QDs onto a carrier can further enhance detection sensitivity. However, traditional carriers such as polystyrene, while commercially successful, involve complex preparation processes and rely on organic solvents, which can lead to fluorescence quenching and adversely affect detection performance. In this study, we selected isoreticular metal–organic framework-3 (IRMOF-3), characterized by its porous structure, high surface area, and abundant amine functional groups, as the carrier. We synthesized IRMOF-3@CdSe/ZnS composite nanoparticles with an average diameter of 378 ± 46 nm by coupling carboxyl-modified CdSe/ZnS QDs to the IRMOF-3 surface through an aqueous synthesis method. These nanocomposites efficiently loaded a high density of QDs without aggregation, thereby maintaining strong fluorescence intensity. Moreover, the abundant amino and carboxyl groups on their surfaces facilitate efficient antibody conjugation, and the nanoparticles retain excellent water dispersibility after labeling. Utilizing IRMOF-3@CdSe/ZnS composite nanoparticles as immunomarkers, we developed a novel fluorescent LFIA for the detection of interleukin-6 (IL-6), a biomarker associated with various inflammatory and autoimmune diseases. The method achieved a limit of detection for IL-6 as low as 8.6 pg/mL, with relative standard deviations ranging from 2.73% to 6.14% and spiking recoveries between 96.4% and 110.8%. Passing-Bablok regression analysis demonstrated good consistency between our method and commercially available immunochromatography assays. The IRMOF-3@CdSe/ZnS composite nanoparticles, as a novel fluorescent label, exhibit considerable potential for applications in biomedical detection. Graphical abstract
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POLYMER ENGINEERING AND SCIENCE
·
2025
·
IF 3.2
Yong Wang,Yanqin Shi,Haojie Li,Meng Ma,Si Chen,Yulu Zhu,Huiwen He,Xu Wang
Epoxy adhesive is becoming increasingly widely used in industry and daily life. There are many requirements to adhere to high and low polar interfaces simultaneously. In this work, a high polar epoxy resin, N , N -bis(4-(oxiran-2-ylmethoxy) phenyl) adipamide (BOYPA), was designed and prepared. The relationship between the curing temperature and the curing time and the curing reaction kinetics equations of BOYPA and Bisphenol A diglycidyl ether (DGEBA) with diethylenetriamine (DETA) was clarified by the study of the curing kinetics according to the Kissinger equation and the Crane equation. Compared with DGEBA, BOYPA required higher curing activation energy and had a lower curing reaction rate. The curing reaction of BOYPA and DGEBA was both a simple curing reaction. Moreover, the peel strength between the PP film and PA6 board bonded by the mixture of BOYPA and DGEBA could be obviously enhanced with the increase of BOYPA content due to the proper polarity and the formation of hydrogen bonds. Meanwhile, the impact strength of the mixture of BOYPA and DGEBA was significantly improved because of the good toughness of BOYPA. As a result, this work provided a simple method for regulating the bonding properties of epoxy adhesive for different adherent surfaces.
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Nanoscale
·
2025
·
IF 5.1
Guanjun Liu,Yan Liu,Meng Zhang,Danyang Zhao,Ping Liu,Lu Wang,Lizhi Li,Meiling Yan
The rise of hydrogen energy places stringent demands on the mechanical strength and thermal conductivity of polyamide 6 (PA6) liners used in hydrogen storage tanks. However, the presence of weak interfaces in PA6 composites significantly hinders the efficient transfer of the intrinsic mechanical and thermal conductivity properties of the reinforcing phases. In this work, hydroxyl-functionalized carbon nanotubes (CNT-OH) were covalently grafted onto graphene oxide (GO) to construct a hybrid nanofiller, which was subsequently surface-functionalized with hexamethylene diisocyanate (HDI), yielding a reactive nanofiller (fGO+CNT-OH+HDI) rich in isocyanate groups. This functionalized nanofiller served as an activator during the in situ anionic ring-opening polymerization of ε-caprolactam, enabling the fabrication of PA6 composites with strengthened nanofiller/nanofiller and nanofiller/matrix interfacial interactions. Owing to the synergistic reinforcement from the hybrid nanofiller and its uniform dispersion driven by in situ polymerization, the resulting composite containing only 0.2 wt% fGO+CNT-OH+HDI exhibited outstanding mechanical performance, with a 36.3% increase in tensile strength and an exceptional elongation at break of 130.86%. In addition, the thermal conductivity was improved by 46.3%. This interfacial engineering strategy provides a promising pathway toward the development of high-performance liner materials for reliable and safe hydrogen energy applications.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiancheng Sun,Chi Zhang,Rubing Zhang
Conventional rigid patch antennas, constrained by high-permittivity substrates ( ε r > 3.0) with severe dielectric loss ( tanδ > 0.02), suffer from catastrophic performance degradation at elevated frequencies (>3 GHz) and inherent inflexibility that fundamentally prohibits their integration in wearable electronics. This work presents a breakthrough in synthesizing PMMA-reinforced polyimide aerogels (PIAs) via a 10-min ambient pressure drying (APD) strategy, enabling tunable porosity (>90%) and ultra-low dielectric constants ( ε r = 1.35, tanδ < 0.01). The incorporation of PMMA into the PI matrix endows the aerogel skeleton with outstanding mechanical resilience (tensile strength of 7.2 MPa and fracture energy of 45.8 kJ/m 2 ), while effectively suppressing capillary-induced shrinkage to below 2.1% during solvent evaporation. The synergistic interplay between PMMA encapsulation and APD-optimized solvent exchange yields a hierarchical nanoporous structure (30 nm average pore size) with exceptional thermal insulation (0.036 W/m·K). When deployed as a substrate for flexible Sub-6 GHz patch antennas, the aerogel exhibits 106% gain enhancement (9.92 dBi) and broad impedance bandwidth (3.862–3.941 GHz), outperforming conventional FR-4 substrates. This work establishes a scalable, low-cost paradigm for multifunctional aerogels in wearable electronics and high-frequency communication systems.
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npj Science of Food
·
2025
·
IF 7.8
Cao Liguang,Li Guojin,Liu Kai,Gao Wenrui,Dong Yifei,Yang Zifeng,Luo Jianchun,Lei Lin,Du Xiliang,Song Yuxiang,Li Xinwei,Gao Wenwen,Liu Guowen
Deoxynivalenol (DON) and fumonisin B1 (FB1) are prevalent mycotoxins commonly found together in dairy cow feed, posing significant risks to cow health and food safety. This study investigates combined toxic effects of DON and FB1 on bovine mammary epithelial cells (BMECs), and role of AMPK-Caspase 6 axis in mycotoxin-induced apoptosis in BMECs and mouse model. We showed that even subcytotoxic doses of DON, combined with FB1, induced synergistic or additive toxic effects, reducing cell viability, increasing LDH release, and promoting apoptosis. This combination also impaired milk triglyceride synthesis and downregulated genes involved in milk fat and protein production. Silencing Caspase 6 via siRNA, inhibiting with Z-VEID-FMK, or activating AMPK with AICAR alleviated apoptosis. Conversely, overexpressing Caspase 6 diminished AMPK’s protective effects. In vivo, inhibiting Caspase 6 alleviated apoptosis in mammary tissues. These findings highlight therapeutic potential of targeting the AMPK-Caspase 6 pathway to mitigate combined toxicity of DON and FB1.
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ANALYTICAL SCIENCES
·
2017
·Vol. 33
·期 5
·585-590
·
IF 1.228
Zhu Xiaoqian,Huang Min,Li Jiao,He Hanping,Zhang Xiuhua,Wang Shengfu
In this paper, a novel electrochemical sensor was developed for the rapid detection of G-G mismatched DNA based on hexaammineruthenium(III) chloride ([Ru(NH 3 ) 6 ]C l3 ) as a redox indicator. The sensor platform was constructed by immobilizing small molecules (NC-linker) on the gold electrode via amide bonds. The as-prepared NC-linker as the nucleic acids recognition molecule can interact with the G base of DNA. After the sensor was incubated with G-G mismatched DNA, the double-stranded DNA (dsDNA) acted as carriers of the signal tags-[Ru(NH3)6]Cl3, which resulted in a remarkable electrochemical signal. More binding of [Ru(NH 3 ) 6 ]C l3 led to increases of the electrochemical signal. Other mismatched DNA produced only a low response, as well as complementary DNA. Thus G-G mismatched DNA can be easily discriminated from other mismatched and complementary DNA based on the sensor. Furthermore, the method was simple, rapid and repeatable for the detection of G-G mismatched DNA. The selective detection of target dsDNA was achieved by a relative current ratio of the target and control DNA. These results demonstrated that this strategy could provide great promise for the rapid and specific detection of other sequence-specific DNA.
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高影响因子
DRUG DEVELOPMENT RESEARCH
·
2021
·Vol. 82
·期 7
·969-979
·
IF 4.36
Ruixian Xing,Xuewen Liu,Buxian Tian,Yan Cheng,Longguang Li
Parkinson's disease (PD) is a disabling neurodegenerative disease mainly caused by degeneration of mesencephalic dopaminergic neurons in the substantia nigra pars compacta (SNpc). The neuroprotective role of Na + /H + exchangers isoform-1 (NHE1) inactivation in cerebral ischemic damage has been elucidated. The current study aimed to investigate the impacts of NHE1 in PD. In this study, 6-hydroxydopamine (6-OHDA)-induced PD rat models were established to attempt to illuminate the role and underlying mechanisms of NHE1 in SNpc neurons of PD. Meanwhile, nerve growth factor-stimulated PC12 cells followed by 6-OHDA treatment was used to mimic PD in vitro. Results showed that the protein levels of NHE1 were significantly increased in the SNpc neurons of rats and differentiated PC12 cells after 6-OHDA treatment. Inactivation of NHE1 with chemical inhibitor HOE642 suppressed SNpc neuronal loss and NHE1 expression in PD rats. The overlays of tyrosine hydroxylase and NHE1 displayed that NHE1 expression was not colocalized but closely associated with TH. Besides, treatment with HOE642 relieved the dyskinesia, mitochondrial dysfunction, and neuronal apoptosis. Further in vitro evidence confirmed that inhibition of NHE1 by genetic-knockdown prevented mitochondrial dysfunction and apoptosis. Our study represents the first experimental evidence of a potential role for NHE1 in the pathogenesis of PD.
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INORGANICA CHIMICA ACTA
·
2024
·
IF 2.7
Shi-Jie Xian,Tao Sun,Yan Li,Kai Wang
Six ternary lanthanide complexes with different β-diketones as primary “antenna” ligand and 2,2’:6’,2’’-terpyridine as neutral ancillary ligand, namely [Ln(hfac) 3 (tpy)] [Ln(III) = Eu ( 1 ), Gd ( 2 ), Tb ( 3 ); hfac = hexafluoroacetylacetone; tpy = 2,2’:6’,2’’-terpyridine] and [Ln(btfa) 3 (tpy)] [Ln(III) = Eu ( 4 ), Gd ( 5 ), Tb ( 6 ); btfa = 4,4,4-trifluoro-1-phenyl-1,3-butanedione], were synthesized and characterized by elemental analysis, infrared spectroscopy, thermogravimetric analysis, single crystal X-ray diffraction and powder X-ray diffraction. The three-dimensional Hirshfeld surface map and two-dimensional fingerprint analysis reveal that the substitution of hfac with btfa result in the decrease of the H···F/F···H and F···F interactions and the increase of the C···H/H···C and H···H interactions. Solid-state luminescence investigations indicate that the Eu(III) complexes ( 1 -Eu and 4 -Eu) and the Tb(III) complexes ( 3 -Tb and 6 -Tb ) display the characteristic lanthanide-centered luminescence upon UV excitations. 1 -Eu and 4 -Eu emit red light at CIE: 0.6827, 0.3172 and CIE: 0.6819, 0.3179 with the quantum yields (QY) of 78.70 % and 68.85 % and the lifetimes ( τ ) of 0.768 and 0.808 ms, respectively. 3-Tb and 6-Tb emit green light at CIE: 0.2677, 0.7221 and CIE: 0.2680, 0.7217 with the QY values of 7.56 % and 1.38 % and the τ values of 0.076 and 0.022 ms, respectively. For the Eu(III) complexes, the substitution of hfac by btfa makes the QY value lower from 78.70 % to 68.85 % and slightly prolong the τ value from 0.768 to 0.808 ms, while for the Tb(III) complexes, both of the QY and τ values decrease. The results suggest that the structures of the primary β-diketone ligands could affect the luminescence performances such as QY and τ values of the Eu(III) and Tb(III) complexes.
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