Frontiers in Aging Neuroscience
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2021
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IF 5.75
Bai Shuju,Wang Wenbo,Zhang Zhiwei,Li Mengyao,Chen Zehan,Wang Jiuqiao,Zhao Yanlin,An Lu,Wang Yuxiang,Xing Shu,Fu Xueqi,Ma Junfeng
Amyloid-β, a hallmark of Alzheimer’s disease, forms toxic intracellular oligomers and extracellular senile plaques resulting in neuronal toxicity. Ethanol is widely consumed worldwide. Moderate ethanol consumption has numerous benefits in humans. We found that ethanol could significantly extend the lifespan of Caenorhabiditis elegans in a previous study. Based on that study, we tested the effect of ethanol on Alzheimer’s disease transgenic Caenorhabiditis elegans strain CL4176, which expresses amyloid-β1-42 peptide in body wall muscle cells. Ethanol delayed paralysis and reduced amyloid-β oligomers in Caenorhabiditis elegans worms of the CL4176 strain. Moreover, ethanol could induce the nuclear translocation of DAF-16 in the nematodes. However, in worms that were fed daf-16 RNAi bacteria, ethanol no longer delayed the paralysis. The qPCR assays showed that ethanol increases the expression of daf-16, hsf-1 and their common target genes- small heat shock protein genes. In addition, we also found that ethanol could increase lysosome mass in the CL4176 worms. In summary, our study indicated that ethanol attenuated amyloid-β toxicity in the Alzheimer’s disease model of Caenorhabiditis elegans via increasing the level of lysosomes to promote amyloid-β degradation and upregulating the levels of small heat shock protein genes to reduce amyloid-β aggregation.
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Journal of Orthopaedic Translation
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2021
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IF 5.191
Wen-Xiang Cheng,Yan-Zhi Liu,Xiang-Bo Meng,Zheng-Tan Zheng,Ling-Li Li,Li-Qing Ke,Ling Li,Cui-Shan Huang,Guo-Yuan Zhu,Hu-Dan Pan,Ling Qin,Xin-Luan Wang,Peng Zhang
Objectives Vascularization is an essential step in successful bone tissue engineering. The induction of angiogenesis in bone tissue engineering can be enhanced through the delivery of therapeutic agents that stimulate vessel and bone formation. In this study, we show that cucurbitacin B (CuB), a tetracyclic terpene derived from Cucurbitaceae family plants, facilitates the induction of angiogenesis in vitro. Methods We incorporated CuB into a biodegradable poly (lactide-co-glycolide) (PLGA) and β-tricalcium phosphate (β-TCP) biomaterial scaffold (PT/CuB) Using 3D low-temperature rapid prototyping (LT-RP) technology. A rat skull defect model was used to verify whether the drug-incorporated scaffold has the effects of angiogenesis and osteogenesis in vivo for the regeneration of bone defect. Cytotoxicity assay was performed to determine the safe dose range of the CuB. Tube formation assay and western blot assay were used to analyze the angiogenesis effect of CuB. Results PT/CuB scaffold possessed well-designed bio-mimic structure and improved mechanical properties. CuB was linear release from the composite scaffold without affecting pH value. The results demonstrated that the PT/CuB scaffold significantly enhanced neovascularization and bone regeneration in a rat critical size calvarial defect model compared to the scaffold implants without CuB. Furthermore, CuB stimulated angiogenic signaling via up-regulating VEGFR2 and VEGFR-related signaling pathways. Conclusion CuB can serve as promising candidate compound for promoting neovascularization and osteogenesis, especially in tissue engineering for repair of bone defects. The translational potential of this article This study highlights the potential use of CuB as a therapeutic agent and strongly support its adoption as a component of composite scaffolds for tissue-engineering of bone repair.
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MICROCHEMICAL JOURNAL
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2023
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IF 4.8
Jianfang Jiang,Kuaqian Deng,Fanglin Dao,Panyang Li,Jiali Huang
Trichlorfon (TCF) is an important chemical for pest and parasite control, but it poses risks to public and environmental health. Thus, the detection of TCF is of great value. In this study, we report a strategy to prepare an AChE-nanoprobe for detecting TCF. Benefiting from the unique and excellent structure of CHO-β-CD, this AChE-nanoprobe can wrap nano-Fe 3 O 4 and covalently bind AChE. As a proof of concept, we prepared an AChE-nanoprobe with a hydrated particle size of 717 nm and a saturation magnetic strength of 64.11 emu/g Fe. The properties were studied in detail. Its catalytic activity was 90 % of that of free AChE, and it had broadened pH, thermal, organic solvents and storage in addition to good reusability, with a residual catalytic activity of 42 % after the 10th cycle and a cumulative catalytic activity 6.9 times higher than that of the free enzyme. The AChE-nanoprobe exhibited good performance in TCF detection; the linear range of TCF was 40–4000 ng/L, and the limit of detection for TCF was 2.8 ng/L. Furthermore, our results suggest that the AChE-nanoprobe has several attractive features, such as easy preparation, outstanding stability and reusability, providing a novel and promising tool for the analysis of TCF.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2011
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IF 3.193
Huang Tang,Li-Zhen Zhao,Hao-Tao Zhao,Shi-Liang Huang,Shu-Ming Zhong,Jiang-Ke Qin,Zhen-Feng Chen,Zhi-Shu Huang,Hong Liang
A series of dual binding site acetylcholinesterase (AChE) inhibitors have been designed, synthesized, and tested for their ability to inhibit AChE, butyrylcholinesterase (BChE), AChE-induced and self-induced β -amyloid (A β ) aggregation. The new hybrids consist of a unit of 1-azabenzanthrone and a tacrine or its congener, connected through an oligomethylene linker containing an amine group at variable position. These hybrids exhibit high AChE inhibitory activity with IC 50 values in the nanomolar range in most cases. Moreover, five out of the 12 hybrids of this series, particularly those bearing a tetrahydroacridine moiety, exhibit a significant in vitro inhibitory activity toward the AChE-induced and self-induced A β aggregation, which makes them promising anti-Alzheimer drug candidates.
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SYNTHESIS-STUTTGART
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2022
·Vol. 54
·期 17
·3719-3728
·
IF 2.969
Xiaokang Kang,Shuyan Lai,Xiayu Liang,Qingle Zeng
1,1-Difunctionalization of α‑carbonyl sulfur ylides and thiosulfonates under simple conditions is disclosed. In this protocol, two new C–S bonds are constructed in a one-step reaction. A series of aliphatic and aromatic β-keto thiosulfones were obtained in moderate to good yields. This reaction probably proceeds through sulfur ylide-involved nucleophilic substitution of an ion pair within a solvent cage.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Feng Wang,Haohao Wang,Kang Kang,Xuan Zhang,Keith Fraser,Fuming Zhang,Robert J. Linhardt
β-Glucosidase is a biological macromolecule that catalyzes the hydrolysis of various glycosides and oligosaccharides . It may also be used to catalyze the synthesis of glycosides under suitable conditions. Carrier-bound β-glucosidase can enhance the enzymatic activity in the synthesis of glycosides in organic solvent solutions, although the molecular mechanism regulating activity is yet unknown. This study investigated the impact of utilizing montmorillonite (Mmt), attapulgite (Attp), and kaolinite (Kao) as carriers on the activity of β-glucosidase from Prunus dulcis (PdBg). When Attp was used as carriers, the molecular dynamic (MD) simulations found the distance between pNPG and the active site residues E183 and E387 was minimally impacted by the adsorptions, hence PdBg maintained about 81.3 ± 0.89 % of its native activity. Out of the three clay minerals, the relative activity of PdBg loaded on Mmt was the lowest because of the highest electrostatic energy. The substrate channel of PdBg on Kao is directed towards the surface, limiting the accessibility of substrates. Secondary structure and conformation studies revealed that the conformational stability of PdBg in solvent solutions was enhanced by coupling to Attp. Unlike dimethyl sulfoxide (DMSO), N , N -dimethylformamide (DMF) and 1,2-dimethoxyethane (DME), tert-butanol ( t -BA) did not penetrate into the active site of PdBg interfering with its binding to the substrate. The maximum yield of n-octyl-β-glucoside (OGP) synthesis catalyzed by Attp-immobilized PdBg reached 48.3 %.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Yibing Xie,Huihui Wu,Jianfei Luo,Shao Zhang,Langlang Wang,Xueqian Wang,Yixing Ma,Ping Ning
The incorporation of vanadium during the synthesis process induced a spontaneous and complete thermal phase transition from α-Bi 2 O 3 to β-Bi 2 O 3 . This phase transition facilitated the development of intrinsic electric field at the interface between V 2 O 5 and β-Bi 2 O 3 , consequently establishing a novel carrier transfer pathway. As a result, the fabricated V 2 O 5 /β-Bi 2 O 3 heterojunction exhibited an outstanding removal efficiency of over 98 % for gaseous mercury (Hg 0 ) under UV light, and this efficiency of 95.0 % even after 35 h of testing. Introducing vanadium enhanced the light absorption capacity in the ultraviolet region and effectively separated photoinduced charge carriers. As revealed by density functional theory calculations, the photoinduced electron–hole transfer in the V 2 O 5 /β-Bi 2 O 3 heterojunction followed the Z-scheme mechanism. This mechanism significantly contributed to the long-term stability of photocatalytic Hg 0 removal, even when H 2 O and NO were present. This study presents a novel material composited by a thermal phase transition technique, aimed at the highly effective and green removal of Hg 0 in the environment.
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JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2023
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IF 5.7
Sehreish Abrar,Faisal Nazeer,Zhuang Ma,Ling Liu,Abdul Malik,Mustafa Kamal,Abdullah G. Al-Sehemi
To raise the operating temperature of Si-based ceramic matrix composites (CMCs), thermal/environmental barrier coating (T/EBCs) materials, typically rare earth silicates are applied. However, the high thermal conductivity of each single silicate possible coating option and the mismatch of thermal expansion present a significant barrier in the development of novel materials to offer thermal insulation and environmental protection to CMCs. The entropy engineering is used to create a multicomponent equi-atomic single-phase pyro silicate with extremely low thermal conductivity and enhanced water vapor corrosion resistance at 1300 °C in 90% H 2 O and 10% O 2 environment, specifically (Yb 0.25 Er 0.25 Tm 0.25 Sc 0.25 ) 2 Si 2 O 7 . X-ray diffraction patterns confirms monophase formation and the thermal parameters such as specific heat capacity, thermal expansion and thermal diffusivity were determined and microstructures were described using scanning electron microscopy before and after water vapor corrosion. The ultra-low thermal conductivity of high entropy disilicates 0.90 W/m.°C at 1000 °C and very low value of weight loss 0.0014 g/cm 2 is observed after 150 h corrosion time which is not reported before for even single or multicomponent high entropy rare earth disilicates.
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MOLECULES
·
2023
·Vol. 28
·期 19
·6879
·
IF 4.6
Tiantian Zhang,Jiacheng Xu,Yan Sun,Shiyu Fang,Zuliang Wu,Erhao Gao,Jiali Zhu,Wei Wang,Shuiliang Yao,Jing Li
Pt-based catalysts exhibit unique catalytic properties in many chemical reactions. In particular, metal–support interactions (MSI) greatly improve catalytic activity. However, the current MSI mechanism between platinum (Pt) and the support is not clear enough. In this paper, the interaction of 1 wt% Pt nanoparticles (NPs) onβ-MnO2in carbon monoxide (CO) oxidation was studied. The Pt onβ-MnO2inhibited CO oxidation below 210 °C but promoted it above 210 °C. A Pt/β-MnO2catalyst contains more Pt4+and less Pt2+. The results of operando DRIFTS-MS show that surface-terminal-type oxygen (M=O) plays an important role in CO oxidation. When the temperature was below 210 °C, Mn=O consumption on Pt/β-MnO2was less thanβ-MnO2due to Pt4+inhibition on CO oxidation. When the temperature was above 210 °C, Pt4+was reduced to Pt2+, and Mn=O consumption due to CO oxidation was greater thanβ-MnO2. The interaction of Pt andβ-MnO2is proposed.Keywords:metal–support interaction;CO oxidation;terminal-type oxygen;bridge-type oxygen;Pt;MnO2
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Jianhua Lv,Yaru Fang,Dazhi Wang,Mi Wu,Wenchang Zhang,Xiaoyu Ou,Huaiguo Li,Lei Shang,Zihong Li,Yan Zhao
Based on the assumption that protein could be removed by the combined mechanism of alkaline induced degradation and strong hydrogen bond interactions of deep eutectic solvents (DESs), β-chitins were successfully prepared from squid pens by using alkaline DESs formed by potassium carbonate and glycerol. The chemical structures of the DESs were investigated by 1 H nuclear magnetic resonance ( 1 H NMR), attenuated total reflection Fourier transform infrared (ATR-FTIR) and molecular modeling , and the physicochemical property of the prepared β-chitins were characterized. The preparation yields was about 32 %, and DESs with K 2 CO 3 /glycerol of 1/10 could be reused for three times while maintaining high preparation yields (31 %–32 %) and degree of deacetylation of 66.9 %–76.9 %. The mechanisms of deproteinization and demineralization by the alkaline DESs were proposed to follow the degradation and dissolution steps, and proteins and minerals were removed from squid pens through the synergistic actions of alkaline degradation and hydrogen bonding interactions. This alkaline DESs are promising to be used as a green and efficient approach for commercial production of β-chitin.
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Water
·
2021
·Vol. 13
·期 21
·3004
·
IF 3.103
Xin Wang,Huafeng Chen,Jianyu Wang,Yi Zhou,Changzheng Cui,Yanbo Zhou
Water pollution caused by organic pollutants and pathogenic microorganism is a critical issue. In this study, we reported a silver-modified β-cyclodextrin polymer (CD-CA/PDA-Ag) used for the removal of dyes and the suppression of microorganisms from water. In order to avoid silver nanoparticles agglomeration, the material was first coated with polydopamine (PDA) on β-cyclodextrin matrix, whose reducing catechol groups subsequently immobilized silver ions in situ to form uniformly dispersed silver nanoparticles. CD-CA/PDA-0.5Ag displayed a high adsorption capacity of methylene blue (535.93 mg/g), which is mainly attributed to electrostatic interactions, host-guest inclusions and π-π bond stacking effects. Furthermore, both diffusion inhibition zone tests and colony formation unit tests were investigated, which showed excellent antimicrobial capability onEscherichia coli(E. coli) andStaphylococcus aureus(S. auerus). Overall, CD-CA/PDA-Ag proved to have an excellent adsorption capacity and antibacterial performance, which provides a great potential in water purification.Keywords:cyclodextrin polymer;adsorption;antibacterial;polydopamine;nano-silver
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ANALYTICA CHIMICA ACTA
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2021
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IF 6.558
Yuwei Bu,Mengjie Zhang,Junliang Fu,Xiaoyan Yang,Shufeng Liu
In this paper, a quantitative cathodic photoelectrochemical aptasensor is described by using black phosphorous quantum dots (BPQDs) as photoactive material and assisted by heme as electron acceptor for sensing of amyloid β peptide (A β ). Specifically, BPQDs were synthesized by solvothermal method and characterized by various techniques. The as-prepared BPQDs were assembled on the transparent indium tin oxide electrode, and the positively charged poly- l -lysine (PLL) was then absorbed onto BPQDs via electronic interaction. Subsequently, the aptamer as the specific recognition element for A β oligomer was introduced on the BPQDs-PLL modified electrode . After bound with heme to form A β -heme complex, A β oligomer was simultaneously captured by the aptamer on the electrode, resulting in an enhanced photocurrent response. Under the optimized conditions, the present PEC sensor reveals a good linear response to A β peptide ranging from 1.0 fM to 100 nM with a detection limit of 0.87 fM. The present signal-on cathodic PEC bioassay possesses the potential to create a new paradigm in amplified PEC assays that could provide outstanding performance for bioanalysis .
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