Journal of Orthopaedic Surgery and Research
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2021
·Vol. 16
·期 1
·1-9
·
IF 2.359
Zhang Hongxia,Wu Guangsheng,Feng Junjian,Lu Xiaohong,Liu Ping
Background This study aimed to investigate the effects of STOML2 and the relationship between STOML2 and PAI-1 in the development of multiple myeloma (MM). Methods Cell proliferation was tested using CCK-8 assay and cell colony formation assay. Glucose consumption, lactate production and ATP level were measured using commercial kits. The mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. Results Both mRNA and protein expression of STOML2 were upregulated in MM patients compared to healthy volunteers. CCK-8 and colony formation assays demonstrated that STOML2 silencing inhibited cell proliferation in MM cells. Knockdown of STOML2 reduced glucose consumption, lactate production and ATP/ADP ratios. STOML2 silencing by shSTOML2 led to reduced PAI-1 expression. Overexpression of PAI-1 reversed the inhibitory effects of shSTOML2 on MM cell growth. Conclusion Results from this study demonstrated that STOML2 silencing inhibits cell proliferation and glycolysis through downregulation of PAI-1 expression, suggesting a new therapeutic target for MM.
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ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 47
·2104830
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IF 18.808
Yuchen Ji,Kai Yang,Mingqiang Liu,Shiming Chen,Xinhua Liu,Biao Yang,Zijian Wang,Weiyuan Huang,Zhibo Song,Shida Xue,Yanda Fu,Luyi Yang,Thomas S. Miller,Feng Pan
Poly(ethylene oxide) (PEO) is a promising solid electrolyte material for solid-state lithium–sulfur (Li–S) batteries, but low intrinsic ionic conductivity, poor mechanical properties, and failure to hinder the polysulfide shuttle effect limits its application. Herein, a polymer of intrinsic microporosity (PIM) is synthesized and applied as an organic framework to comprehensively enhance the performance of PEO by forming a composite electrolyte (PEO-PIM). The unique structure of PIM-1 not only enhances the mechanical strength and hardness over the PEO electrolyte by an order of magnitude, increasing stability toward the metallic lithium anode but also increases its ionic conductivity by lowering the degree of crystallinity. Furthermore, the PIM-1 is shown to effectively trap lithium polysulfide species to mitigate against the detrimental polysulfide shuttle effect, as electrophilic 1,4-dicyanooxanthrene functional groups possess higher binding energy to polysulfides. Benefiting from these properties, the use of PEO-PIM composite electrolyte has achieved greatly improved rate performance, long-cycling stability, and excellent safety features for solid-state Li-S batteries. This methodology offers a new direction for the optimization of solid polymer electrolytes.
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Biomed Research International
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2021
·
IF 3.411
Ruixi Luo,Lifeng Zhao,Shuaishuai Li,Peng Chen,La Wang,Honghong Yu,Kun Cai,Qi Yu,Weiyi Tian
Excessive free fatty acid- (FFA-) induced endothelial lipotoxicity is involved in the pathogenesis of atherosclerosis. Endoplasmic reticulum (ER) stress is mechanistically related to endothelial lipotoxicity. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is the major oxidatively modified low-density lipoprotein (OxLDL) receptor in endothelial cells and is highly abundant in atherosclerotic lesions. Curcumin reduces the LOX-1 expression; however, the mechanism underlying this effect remains unknown. In the current study, we explored whether curcumin ameliorates palmitic acid- (PA-) induced endothelial lipotoxicity and LOX-1 upregulation by reducing ER stress in human umbilical vein endothelial cells (HUVECs). We built endothelial lipotoxicity in vitro and found that LOX-1 was upregulated after PA stimulation, during which ER stress played an important role. Next, we observed that curcumin substantially alleviated PA-induced lipotoxicity by restoring cell viability, increasing angiogenesis, and decreasing lipid deposition. Furthermore, LOX-1 upregulation in HUVECs was blocked by curcumin, possibly via ER stress suppression. Overall, our findings demonstrated that curcumin alleviates endothelial lipotoxicity and LOX-1 upregulation, and ER stress inhibition may play a critical role in this effect.
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ACTA PHARMACOLOGICA SINICA
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2021
·Vol. 43
·期 5
·1264-1273
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IF 6.15
Ren Zheng-nan,Yang Jun,Zhang Meng-ya,Huang Yi-wen,Song Dong-xiao,Sun Xun,Pan Li-long,Sun Jia
Acute pancreatitis (AP), an inflammatory disorder of the pancreas, is a complicated disease without specific drug therapy. ( R )-4,6-dimethoxy-3-(4-methoxy phenyl)-2,3-dihydro-1H-indanone [( R )-TML104] is a synthesized analog of the natural product resveratrol sesquiterpenes (±) -isopaucifloral F. This study aimed to investigate the effect and underlying mechanism of ( R )-TML104 on AP. The experimental AP model was induced by caerulein hyperstimulation in BALB/c mice. ( R )-TML104 markedly attenuated caerulein-induced AP, as evidenced by decreased pancreatic edema, serum amylase levels, serum lipase levels, and pancreatic myeloperoxidase activity. In addition, ( R )-TML104 significantly inhibited the expression of pancreatic chemokines C–C motif chemokine ligand 2 and macrophage inflammatory protein-2 and the infiltration of neutrophils and macrophages. Mechanistically, ( R )-TML104 activated AMP-activated protein kinase and induced sirtuin 1 (SIRT1) expression. ( R )-TML104 treatment markedly induced the SIRT1-signal transducer and activator of transcription 3 (STAT3) interaction and reduced acetylation of STAT3, thus inhibiting the inflammatory response mediated by the interleukin 6-STAT3 pathway. The effect of ( R )-TML104 on SIRT1-STAT3 interaction was reversed by treatment with a SIRT1 inhibitor selisistat (EX527). Together, our findings indicate that ( R )-TML104 alleviates experimental pancreatitis by reducing the infiltration of inflammatory cells through modulating SIRT1.
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FUEL
·
2021
·
IF 6.609
Mingjie Liao,Xin Ning,Junwen Chen,Jiajun Zheng,Wenlin Li,Ruifeng Li
Zeolite is one kind of the solid acid catalysts for the oligomerization process but surfers from rapid deactivation. In this specific study, a series of mesoporous ZSM-5 catalysts have been synthesized and evaluated in the 1-hexene oligomerization. XRD, BET, SEM, TEM, 27 Al MAS NMR, 29 Si MAS NMR, NH 3 -TPD, Py-FTIR and TGA analysis were used to characterize the physicochemical properties of zeolites. The synthesized mesoporous zeolites were found to be more stable in the reaction and the initial activity increase significantly compared with the common ZSM-5 zeolite . Moreover, the catalytic activity of mesoporous ZSM-5 zeolite is found to be closely related to the number and the distribution of acid sites. The mesoporous ZSM-5 zeolites with the lower amount of acid sites, moderate strength of Brønsted acid sites inhibiting the cracking and coke products. High L/B ratio enhanced the dimers formation. The efficiency was further highlighted by a high space–time yield (STY) of 557 mg·g cat −1 h −1 in the average stage at 260 °C. Compared to conventional ZSM-5 catalyst, the synthesized catalyst displayed excellent resistance to coke deposit.
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APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Qing Sun,Meilin Ke,Yingjie Zhao,Bin Wang,Jian Zhang,Jiawei Sheng
Improving the ability of photocatalysts to capture and degrade dye pollutants is essential for the development of photocatalytic technology. Herein, a Bi 2 MoO 6 belt-like morphology with significantly enhanced visible light photocatalytic performance was obtained by solvent hydrothermal combining ethanol heat treatment technique. The Raman, FTIR, TEM, ESR, EIS and PL results demonstrate that the formation of defects on the {0 0 1} facet of BMO can effectively promote charge separation and prolong the life of optical carriers. The photocatalytic performance is evaluated by monitoring different concentrations RhB removal from aqueous solution and the results showed that defective Bi 2 MoO 6 nanobelts displayed 39 times and 60 times degradation rate than that of comparative conventional Bi 2 MoO 6 nanosheets and P25, respectively. In combination of controllable preparation process, excellent adsorption capacity and highly enhanced photocatalytic performance, this newly defective Bi 2 MoO 6 nanobelt might be a promising candidate for degradation of organic dyes in wastewater.
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ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2021
·
IF 4.013
Weihua Wu,Qian Chai,Ziying Zhang
Recent studies have shown that blood glucose fluctuation is associated with complications of diabetes mellitus (DM). SGLT1 (sodium-dependent glucose cotransporter 1), is highly expressed in pathological conditions of heart, and is expressed in cardiomyocytes induced by high glucose. Herein, we constructed a diabetic mouse model with glucose fluctuation to investigate whether SGLT1 is involved in glucose fluctuation-induced cardiac injury. Echocardiography, histology examination, and TUNEL staining were performed to evaluate cardiac dysfunction and damage. To assess glucose fluctuation-induced oxidative stress , reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels were measured. To assess mitochondrial dysfunction, mitochondrial membrane potential (MMP), ATP content, mitochondrial respiratory chain complex activity, and expression of mitochondrial fusion and fission proteins were determined. The results indicated that diabetic mice with glucose fluctuation showed elevation of cardiac SGLT1 expression, left ventricular dysfunction , oxidative stress and mitochondrial dysfunction. Knockdown of SGLT1 could abrogate the effects of glucose fluctuation on cardiac injury. Thus, our study highlighted that SGLT1 plays an important role in glucose fluctuation induced cardiac injury through oxidative stress and mitochondrial dysfunction.
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Journal of Environmental Sciences
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2021
·
IF 5.565
Li Xiang,Chaoyang Liu,Deng Liu,Liyuan Ma,Xuan Qiu,Hongmei Wang,Xiaolu Lu
Soils and waters are heavily contaminated by antimony in Xikuangshan (XKS) mine area. It is widely accepted that oxidative dissolution of sulfide minerals and aqueous dissolution are the most prevalent geochemical mechanisms for the release of Sb to the environment. Bosea sp. AS-1 is an antimonite-oxidizer isolated from the mine slag in Xikuangshan Sb mine. Whole genome sequencing revealed the presence of multiple sulfur-oxidizing genes, antimony (Sb) metabolism genes and carbon fixation genes in AS-1′s genome. We therefore hypothesized that under oxic conditions, AS-1 could mediate the oxidation of sulfide and Sb(III) in stibnite (Sb 2 S 3 ) and lead to the release of Sb. Indeed, strain AS-1 was discovered as an autotrophic Sb(III)-oxidizer. Antimony mobilization studies conducted with strain AS-1 showed significantly enhanced mobilization of Sb, and complete oxidation of released Sb and sulfur to Sb(V) and sulfate. In addition, AS-1 induced a faster release of Sb under heterotrophic condition, and new acicular minerals might form. These findings support the hypothesis that microorganisms play an important role in the mobilization and transformation of Sb in XKS mine area and may contribute to our further understanding of the Sb biogeochemical redox cycle in natural environment.
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International Journal of Greenhouse Gas Control
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2021
·
IF 3.738
Qiangwei Li,Ge Gao,Rujie Wang,Shihan Zhang,Shanlong An,Lidong Wang
Biphasic solvents are promising candidates for CO 2 capture for low regeneration energy consumption. In this study, a novel phase splitter 1-methylimidazole (1-MI) was proposed for triethylenetetramine (TETA) aqueous solvent. 1-MI was proved to act as a phase splitter to induce the phase separation and as an absorption promoter to enhance the absorption rate. By tuning the concentrations of TETA and 1-MI, good phase separation performance was obtained in TETA-1-MI biphasic solvent, achieving a CO 2 -rich phase loading of 5.41 mol/L. 13 C NMR revealed that the upper layer was the CO 2 -lean phase, containing 1-MI and a small amount of water, while the lower layer was the CO 2 -rich phase and mainly comprised of TETAH + and TETA-carbamate. The kinetics results based on the double-film theory indicated that the CO 2 absorption rate was mainly controlled by the gas film mass transfer resistance at lower CO 2 loading and then by liquid film mass transfer resistance when CO 2 loading was higher than 1 mol/L. Owing to the good phase separation behavior triggered by 1-MI, the regeneration heat of TETA-1-MI was 2.26 GJ/t CO 2 , which was comparable to that of the second-generation solvents, and 43.5 % lower than that of 5 M monoethanolamine (MEA). The present findings indicated that TETA-1-MI had a large loading capacity, high absorption rate, and low energy consumption, which can serve as a novel candidate for CO 2 capture.
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ENVIRONMENTAL MICROBIOLOGY
·
2021
·Vol. 23
·期 5
·2550-2563
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IF 5.491
Shunyao Li,Kai Sun,Xin Yan,Chao Lu,Michael Gatheru Waigi,Juan Liu,Wanting Ling
Summary Novosphingobium sp. ES2-1 is an efficient 17 β -estradiol (E2)-degrading bacterium, which can convert E2 to estrone (E1), then to 4-hydroxyestrone (4-OH-E1) for subsequent oxidative cracking. In this study, the molecular bases for this process were elucidated. Two novel monooxygenase systems EstP and EstO were shown to catalyse the oxygenation of E1 and 4-OH-E1, respectively. EstP was a three-component cytochrome P450 monooxygenase system consisting of EstP1 (P450 monooxygenase), EstP2 (ferredoxin) and EstP3 (ferredoxin reductase). Ultraperformance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) analysis revealed that EstP catalysed the 4-hydroxylation of E1 to produce 4-OH-E1. The resultant 4-OH-E1 was further oxidized by a two-component monooxygenase system EstO consisting of EstO1 (flavin-dependent monooxygenases) and EstO2 (flavin reductase). UPLC-HRMS combined with 1 H-nuclear magnetic resonance analysis demonstrated that EstO catalysed the breakage of C9-C10 to yield a ring B-cleavage product. In addition, the oxygenase component genes estP1 and estO1 exhibited contrary inductive behaviours when exposed to different steroids, suggesting that EstP1-mediated 4-hydroxylation was E2-specific, whereas EstO1-mediated monooxygenation might be involved in the degradation of testosterone, androstenedione, progesterone and pregnenolone. This also implied that the mechanisms of the catabolism of different steroids by the same microorganism might be partially interlinked.
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Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-12
·
IF 5.328
Jia Hexue,Sun Wan,Li Xuezhi,Zhao Jian
Background Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 (Cip1) is one of the most abundant proteins in extracellular proteins of T. reesei. Reported literatures about Cip1 mainly focused on the regulation of Cip1 and its possible enzyme activities, but the effect of Cip1 on the enzymatic hydrolysis of lignocellulose and possible mechanism have not still been reported.Results In this study, Cip1 from T. reesei was cloned, expressed and purified, and its effects on enzymatic hydrolysis of several different pretreated lignocellulose were investigated. It was found that Cip1 could promote the enzymatic hydrolysis of pretreated lignocellulose, and the promoting effect was significantly better than that of bovine serum albumin (BSA). And especially for the lignocellulosic substrate with high lignin content such as liquid hot water pretreated corn stover and corncob residue, the promoting effect of Cip1 was even better than that of the commercial cellulase when adding equal amount protein. It was also showed that the metal ions Zn2+ and Cu2+ influenced the promoting effect on enzymatic hydrolysis. The Cip1 protein had no lyase activity, but it could destroy the crystal structure of cellulose and reduce the non-productive adsorption of cellulase on lignin, which partly interpreted the promoting effect of Cip1 on enzymatic hydrolysis of lignocellulose.Conclusion The Cip1 from T. reesei could significantly promote the enzymatic hydrolysis of pretreated lignocellulose, and the promotion of Cip1 was even higher than that of commercial cellulase in the enzymatic hydrolysis of the substrates with high lignin content. This study will help us to better optimize cellulase to improve its ability to degrade lignocellulose, thereby reducing the cost of enzymes required for enzymatic hydrolysis.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Chen Jin,Jian Sun,Yuning Chen,Yafei Guo,Donghui Han,Ruilin Wang,Chuanwen Zhao
Sawdust wastes were used as the low-cost feedstock to prepare porous carbons by KOH activation, and the preparation parameters were optimized via orthogonal experiment. Four main activation parameters (i.e., sawdust type, KOH/sawdust ratio, activation temperature and activation time) were investigated. It is found that activation temperature and KOH/sawdust ratio are the two most important parameters that affect the porous texture properties (specific surface area and micropore volume) of sawdust wastes-derived porous carbons. The optimal sawdust wastes-derived porous carbon possesses a high specific surface area of 1601 m 2 /g and a micropore volume of 0.75 cm 3 /g, therefore exhibiting a desirable CO 2 adsorption capacity of 4.25 mmol/g at 0 °C under ambient pressure. Comparatively, the sawdust wastes-derived porous carbon extrudates exhibit the inferior CO 2 adsorption capacity, dropping to 2.85–3.03 mmol/g. It is mainly attributed to the extrusion treatment and binder addition causing the collapse and blockage of the original porous structure, consequently increasing the CO 2 diffusion and adsorption resistance. However, the sawdust wastes-derived porous carbon extrudates possess the advantages of null pressure build-up, improved bulk density and high compress strength (7.3–12.3 MPa) considering the real application point of view.
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