MOLECULAR CRYSTALS AND LIQUID CRYSTALS
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2016
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IF 0.532
J. Wei,K. Liu,F. Lin,Y. Zhou,J. Zou,C. Lin
Through the sulfonation of protocatechuic acid, sodium 5-carboxy-2,3-dihydroxybenzenesulfonate (SPA) was synthesized. The structure of the sulfonated derivative was confirmed by FT-IR, MS, 1H NMR, 13C NMR, single crystal X-ray diffraction, and thermogravimetric analysis. The antioxidative activities of the derivative SPA were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging method and reducing power determination, showing that SPA had strong antioxidative effects. Meanwhile DPPH scavenging progress by SPA was analyzed from dynamics experiments. Isothermal titration calorimetry (ITC) and circular dichroism (CD) were applied to investigate the interaction between the derivative SPA and human serum albumin (HSA).
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SOUTH AFRICAN JOURNAL OF BOTANY
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2016
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IF 1.244
H. Li,D. Zhang,L.-H. Tan,B. Yu,S.-P. Zhao,W.-G. Cao
In this study, Dipsacus asperoides was separately extracted in three solvents (water, methanol and acetone), and the antioxidant activities of each extract were investigated through assays of DPPH, ABTS, β-carotene/linoleic acid system and reducing power. The total phenolic acids, flavonoids and saponins in each solvent extract were calculated for a correlation analysis. The phenolic compounds in the extracts were identified by LC-ESI-QTOF-MS–MS. The results showed that the three extracts exhibited antioxidant effects in a concentration-dependent manner, and the water extracts exerted the highest antioxidant activity effect followed by the methanol and acetone extracts. The correlation analysis indicated that the total phenolic components presented the greatest contribution to the antioxidant activities. Fifteen main phenolic compounds were identified in the water extracts, and three of these compounds had not been previously identified in D. asperoides . The results suggest that water extraction of D. asperoides is a promising source of natural antioxidants.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
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IF 2.857
Guang Wang,Hong Zhang,Furao Lai,Hui Wu
Selenite can enhance the selenium nutrition level of crops, but excessive selenite may be toxic to plant growth. To elucidate the mechanisms underlying the role of selenite in production and detoxification of oxidative toxicity, peanut seedlings were developed with sodium selenite (0, 3, and 6 mg/L). The effects of selenite on antioxidant capacity, transcript levels of antioxidant enzyme genes, and enzyme activities in hypocotyl were investigated. The CuZn-SOD, GSH-Px, GST, and APX gene expression levels and their enzyme activities in selenite treatments were 1.0–3.6-fold of the control. Selenite also significantly increased the glutathione and ascorbate concentrations by mediating the ascorbate–glutathione cycle, and the selenite-induced hydrogen peroxide may act as a second messenger in the signaling pathways. This work has revealed a complex antioxidative response to selenite in peanut seedling. Understanding these mechanisms may help future research in increasing selenite tolerance and selenium accumulation in peanut and other crops.
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PubMed
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2016
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IF 3.138
Hong Niu,Dan Song,Haibo Mu,Wuxia Zhang,Feifei Sun,Jinyou Duan
Mushroom Inonotus obliquus ( I. obliquus ), a folk medicine, has been widely used to treat several human malicious tumors since 16th century. In this study, three homogenous biomolecules (designated IOA1, IOA2 and IOA3) were prepared from the alkali extract of I. obliquus . Their molecular weights were measured to be 6.1 × 10 4 , 2.9 × 10 4 and 3.5 × 10 4 g/mol respectively and all of them were characterized as lignin–carbohydrate complexes mainly comprised lignin as well as −25% carbohydrates. Antioxidant assays indicated that all of them exhibited pronounced reductive power and strong scavenging activities on DPPH and hydroxyl radicals in vitro . Immunological tests showed that they could also significantly stimulate nitric oxide production and phagocytic activity in RAW 264.7 macrophages. These results implied that the lignin–carbohydrate complexes extracted from I. obliquus might be used as novel natural antioxidants or immunostimulants in functional foods or pharmaceutical candidates.
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PubMed
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Journal of Functional Foods
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2016
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IF 3.973
Limin Xiang,Yihai Wang,Xiaomin Yi,Xinluan Wang,Xiangjiu He
Chinese hickory ( Carya cathayensis Sarg.) is a common and nutritious nut. Twenty-nine phenolic compounds, together with three quinones and three triterpenoids , were isolated from Chinese hickory husks. Their structures were determined by comprehensive spectroscopic methods. The total methanolic extract, chloroform and ethyl acetate fractions and all the isolated compounds were evaluated for antioxidant activity and neuroprotective effects on SH-SY5Y cells, as well as the effects on the expression of PPAR-γ in 3T3-L1 cells. Quercetin ( 10 ) and protocatechuic acid methyl ester ( 24 ) showed pronounced antioxidant activities in all of the different antioxidant assays and neuroprotective effect against H 2 O 2 -induced oxidative stress . Some of the flavonoids could significantly decrease the mRNA expression level of PPAR-γ by approximately 70%. The present study provided supplement information for the evaluation of antioxidant activity and health benefit of Chinese hickory.
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RSC Advances
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2016
·Vol. 6
·期 37
·31374-31385
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IF 3.289
Hongshan Liang,Yaqiong Pei,Jing Li,Wenfei Xiong,Yun He,Shilin Liu,Yan Li,Bin Li
Hydrogen-bonded polyphenol-based assemblies have attracted increasing interest for biomedical applications. Polyphenolic drug-loaded films can be coated on various devices with different shapes and sizes. Here, we report a novel versatile pH-responsive system based on hydrogen-bonded poly(ethylene glycol) (PEG)/tannin acid (TA) coatings on zein/TA colloidal nanoparticles (zein/TA/PEG NPs). Hydrogen bonding was considered to be the driving force for the coating buildup between PEG and TA, which was confirmed by Fourier transform infrared (FTIR) spectra. Because of the reversible/dynamic nature of hydrogen bonding, the release profile of TA was directed by pH value, temperature, ionic strength and TA concentration. The release rate of TA increased with increasing pH and temperature, but decreased with increasing ionic strength. This new drug delivery vehicle could also be used to load hydrophobic and unstable molecules through interacting with zein by hydrophobic interaction to achieve efficient protection. We demonstrated that the hydrophobic molecular nutrient, VD3, can be successfully loaded into zein/TA/PEG NPs with high encapsulation efficiency. Photostability against UV light was significantly improved after encapsulation. The encapsulated VD3 could be regulated not only by the pH of the solutions but also by TA concentration.
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CARBOHYDRATE POLYMERS
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2016
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IF 4.219
Yongqin Wei,Jin Zhang,Yan Zhou,Weiya Bei,Yuan Li,Qipeng Yuan,Hao Liang
Various biological activities of glabridin have been reported in literature, however, the bioavailability and clinical application was limited by its low water solubility. In this study, we developed a novel inclusion complex of glabridin and hydroxypropyl-β-cyclodextrin (HP-β-CD) that features improved solubility and bioactivity. The formation of the inclusion complex was supported by data from FT-IR, PXRD, SEM and NMR. Specifically, the NMR results indicated that the aromatic ring of glabridin was merged into and located at the narrow side the cavity of HP-β-CD. The in vitro tests showed that the formation of the inclusion complex led to 9 times increase in the DPPH radical-scavenging capacity and 20 times increase in the tyrosinase inhibitory activity compared to the free glabridin at the same concentration. Therefore, the glabridin/HP-β-CD inclusion complex adds a promising strategy for the clinical application of glabridin in the future.
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POLYMER BULLETIN
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2016
·Vol. 74
·期 3
·641-656
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IF 1.371
Yang Xiao,Duan Lian,Ran Xiaoqi
The effect of polydopamine coating on improving photostability of polyphenylene sulfide (PPS) fiber was investigated in detail. Due to strong adhesion ability and self-polymerization of dopamine in alkaline solution, the polydopamine could be easily coated on polyphenylene sulfide fiber under mild conditions. XPS, ATR-FTIR and SEM, which indicated the polydopamine coating was homogeneous, confirmed the polydopamine coating on PPS fiber. The UV-spectra analysis of polydopamine coating showed that it had a strong absorbance in ultraviolet wavelength and was very stable to it, which implied that the polydopamine was a potential photoprotection agent. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) experiment further revealed that the PDA could quench free radicals, suggesting PDA was a good antioxidant. This scavenging ability of PDA might contribute to improving photostability of PPS fiber. Compared to pristine PPS fiber, the PPS fiber coated by polydopamine obviously had better photostability after ultraviolet irradiation according to mechanical test, which proved that polydopamine coating did improve the photostability of PPS fiber.
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Scientific Reports
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2017
·Vol. 7
·期 1
·1-13
·
IF 4.259
Zhao Wenwen,Feng Haitao,Guo Shuhui,Han Yantao,Chen Xiuping
ICAM-1 overexpression and subsequent adhesion of leukocytes to endothelial cells play critical roles in the early stage of atherosclerosis. Danshenol A (DA) is an abietane-type diterpenoid isolated from traditional Chinese herb Salvia miltiorrhiza Bunge. The mechanisms under its regulation of adhesion of molecular expression are explored. Here, the effect of DA on TNF-α-induced ICAM-1 expression was investigated in endothelial cells. TNF-α-induced ICAM-1 expression and subsequent adhesion of monocytes, as well as elevated reactive oxygen species (ROS) generation and NOX4 expression were all significantly reversed by DA, siNOX4 and NOX4 inhibitor GKT137831. Furthermore, TNF-α-induced ICAM-1 expression, which was increased via IKKβ/IκBα-mediated activation of NF-κB p65, was also inhibited by DA. Interestingly, NOX4 overexpression suppressed the ICAM-1 expression, and this finding may be ascribed to the activation of Nrf-2. Additionally, NF-κB inhibitor PDTC, siNOX4, or DA can decrease the TNF-α-induced ICAM-1 expression and suppress the adhesion of monocytes. In all, DA inhibited TNF-α-induced ICAM-1 expression and subsequent monocyte adhesion to endothelial cells through the NOX4-dependent IKKβ/NF-κB pathway. Besides, NOX4 played dual role in regulating ICAM-1 expression via diverse signal pathway. This novel bioactivity will make DA a good candidate to be further explored for therapeutic or preventive application for atherosclerosis.
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Journal of Food Measurement and Characterization
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2017
·Vol. 11
·期 4
·1947-1958
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IF 0.536
Zhao Zhenyu,Liu Pinghuai,Wang Shenglin,Ma Shasha
Ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) were developed to extract flavonoids antioxidants from the root of Millettia speciosa ( Millettia speciosa Champ.). The optimal experimental parameters for antioxidant extraction from M. speciosa Champ. were measured using single-factor experimentation combined with response surface methodology (RSM). Final extraction rate (FER) of flavonoids assay was used to quantify antioxidant compounds. Then, 2,2ˊ-diphenyl-1-picryhydrazyl (DPPH) scavenging capacity, 2,2ˊ-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) scavenging capacity and ferric reducing antioxidant power (FRAP) were used to determine the antioxidant capacities of extract. Besides, UAE and MAE were compared with conventional Soxhlet extraction (CSE) and M. speciosa Champ. powders were observed by scanning electronic microscopy to analyze the damage of cell structure caused by different extraction methods. The results showed that UAE was the optimal extraction method to extract flavonoids compounds from M. speciosa Champ. and the optimized UAE conditions of flavonoids was as follows: ethanol, 80%, temperature, 40 °C and ultrasonic power, 500 W. This paper provided useful information to utilize the resource of Chinese herbal medicine and manifest the great antioxidant activities of M. speciosa Champ., and the optimized UAE conditions of flavonoids from M. speciosa Champ. could be used in industrial production.
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JOURNAL OF MOLECULAR LIQUIDS
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2017
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IF 3.648
Yongqin Wei,Jin Zhang,Amjad Hussain Memon,Hao Liang
Phloretin , a dihydrochalcone flavonoid , has been associated with a wide range of biological properties such as antioxidation, antitumor , antidiabetes, skin whiting, etc. However, the instability and aqueous insolubility in its native form decreases its bioavailability and make its usage limited in clinical application. In our study, an inclusion complex of phloretin with hydroxypropyl-β-cyclodextrin (HP-β-CD) that featured improved aqueous solubility and stability was prepared using a simple co-evaporation method. The inclusion complex prepared under a condition of 1:1, 50 °C, 2 h and 500 rpm showed a great enhancement of phloretin aqueous solubility of 54.08 ± 0.05 mg/mL, which was 5808 times higher than that of free phloretin in pure water at 25 °C. The results of phase solubility study and the characterization data from SEM, PXRD , FT-IR and NMR suggested that aromatic ring of one phloretin molecule was merged into the cavity of one HP-β-CD from the narrow side. The in vitro test showed that the inclusion complex still remained an approximately DPPH radical-scavenging capacity compared to the free phloretin at the same concentration. And the stability of phloretin was greatly improved after forming inclusion complex with HP-β-CD. Thus, the phloretin/HP-β-CD inclusion complex would be a promising approach in the clinical application of phloretin in the future.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2017
·Vol. 52
·期 5
·1176-1184
·
IF 1.64
Jing Li,Wenting Shang,Xu Si,Dandan Bu,Padraig Strappe,Zhongkai Zhou,Chris Blanchard
Summary Corn bran polysaccharides were extracted using an ultrasonic-assisted method with a yield of 33.50% and then carboxymethylated. The occurrence of modification was confirmed using Fourier transform infrared spectral analysis. The surface of polysaccharide particles became loosened following the carboxymethylation. The effect of sodium hydroxide concentration, reaction temperature, and the reaction time on the degree of substitution was investigated, and the relationship among the substitution degree, total phenolic content and antioxidant activity were studied. With sodium hydroxide concentration of 6.0 mol L −1 , keeping the reaction temperature of 60 °C and reaction time of 5.0 h, the modified polysaccharides possessed the highest degree of substitution and antioxidant activity. Cell culture model indicated that the carboxymethylated polysaccharide significantly inhibited the proliferation of A549 and HepG-2 cells, and these antitumour characteristics may be associated with the elevated expression of CASP3 , CASP8 , CASP9 , and p53 , as well as the significantly decreased expression of Bcl-2 and iNOS genes. The excellent antioxidant capacity and strong antitumour activity of these polysaccharides might provide a potential application as a naturally functional ingredient.
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