MOLECULES
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2022
·Vol. 27
·期 9
·2809
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IF 4.927
Qi-Ting Fang,Wen-Wen Luo,Ya-Nan Zheng,Ying Ye,Mei-Juan Hu,Xin-Qiang Zheng,Jian-Liang Lu,Yue-Rong Liang,Jian-Hui Ye
Chemicals underlying the floral aroma of dry teas needs multi-dimensional investigations. Green, black, and freeze-dried tea samples were produced from five tea cultivars, and only ‘Chunyu2’ and ‘Jinguanyin’ dry teas had floral scents. ‘Chunyu2’ green tea contained the highest content of total volatiles (134.75 μg/g) among green tea samples, while ‘Jinguanyin’ black tea contained the highest content of total volatiles (1908.05 μg/g) among black tea samples. The principal component analysis study showed that ‘Chunyu2’ and ‘Jinguanyin’ green teas and ‘Chunyu2’ black tea were characterized by the abundant presence of certain alcohols with floral aroma, while ‘Jinguanyin’ black tea was discriminated due to the high levels of certain alcohols, esters, and aldehydes. A total of 27 shared volatiles were present in different tea samples, and the contents of 7 floral odorants in dry teas had correlations with those in fresh tea leaves (p< 0.05). Thus, the tea cultivar is crucial to the floral scent of dry tea, and these seven volatiles could be promising breeding indices.Keywords:floral scent;tea cultivars;processing;volatiles;alcohols;principal component analysis;co-expression analysis
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AQUATIC TOXICOLOGY
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2022
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IF 5.202
Xia Zhao,Xu Shi,Qingqing Liu,Xiaojing Li
Overuse of acetochlor pollutes soil and rivers, causing threats to the ecosystem. Studies found that acetochlor exposure could damage multiple organs and tissues in fish and mammal. Tea polyphenols (TP), a natural antioxidant that extracted from tea, has been widely used in food and feed additions. However, the mechanism by which acetochlor causes tissue damage is unclear, and its mitigating agent has yet to be developed. Therefore, we established acetochlor exposure and TP mitigation models by treating Ctenopharyngodon idellus kidney (CIK) cells with 20 μM acetochlor and/or 2.5 μg/mL TP for 24 h, and detected the programmed cell death and its related pathways. The results showed that acetochlor exposure modified antioxidant enzyme activities, induced oxidative stress, resulted in the decline of MMP and ATP levels, enhanced glycolysis and lactate accumulation, and triggered apoptosis and necroptosis in CIK cells. However, TP could inhibit CYP450s expression, activate Nrf2 pathway, enhance antioxidant capacity, further effectively alleviate acetochlor-induced CIK cell death. Overall, the present study proved that acetochlor exposure triggered mitochondrial damage and lactate accumulation-mediated apoptosis and necroptosis through CYP450s/ROS/MAPK/NF-κB pathway. Furthermore, TP could alleviate effectively cell death through relieving oxidative stress and lightening Warburg-like effect.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2022
·
IF 4.52
Alberta Osei Barimah,Ping Chen,Limei Yin,Hesham R. El-Seedi,Xiaobo Zou,Zhiming Guo
Detrimental health effects caused by the intake of food contaminated with heavy metals have drawn concerns on effective monitoring using rapid and benign methods. This work presented a novel surface-enhanced Raman scattering (SERS)-based 3-Aminobenzeneboronic acid (ABBA) labeled silver (Ag) nanosensor combined with chemometric algorithms to detect and predict total arsenic (TAs) in acid digested spiked black tea leaves. The sensor recognizes TAs through the partial detachment of ABBA and the chemical formation of As-O-Ag linkage between the TAs and the Ag nanoparticles , which caused a SERS-on signal enhancement effect. SERS combined with competitive adaptive reweighted sampling partial least squares algorithm predicted the TAs with higher correlation coefficient (R p ) results (R p = 0.9750) and a detection limit of 0.0273 µg/g. Good recoveries of 83.84–109.53% and the excellent agreement with the inductively coupled plasma-mass spectrometry method (R 2 = 0.999) revealed this developed rapid method could be deployed for fast-tracking of As in food samples.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2022
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IF 5.437
Dahe Qiao,Mengsha Tang,Ling Jin,Xiaozeng Mi,Hongrong Chen,Junyan Zhu,Shengrui Liu,Chaoling Wei
Monoterpenes and sesquiterpenes are the most abundant volatiles in tea plants and have dual functions in aroma quality formation and defense responses in tea plants. Terpene synthases (TPS) are the key enzymes for the synthesis of terpenes in plants; however, the functions of most of them in tea plants are still unknown. In this study, six putative terpene biosynthesis gene clusters were identified from the tea plant genome. Then we cloned three new TPS-b subfamily genes, CsTPS08 , CsTPS10 and CsTPS58 . In vitro enzyme assays showed that CsTPS08 and CsTPS58 are two multiple-product terpene synthases, with the former synthesizing linalool as the main product, and β-myrcene, α-phellandrene, α-terpinolene, D-limonene, cis-β-ocimene, trans-β-ocimene and (4E,6Z)-allo-ocimene as minor products are also detected, while the latter catalyzing the formation of α-pinene and D-limonene using GPP as the substrate. No product of CsTPS10 was detected in the prokaryotic expression system , but geraniol production was detected when transiently expressed in tobacco leaves. CsTPS08 and CsTPS10 are two functional members of a monoterpene synthase gene cluster, which were significantly induced during both Ectropis oblique feeding and fresh leaf spreading treatments, suggesting that they have dual functions involved in tea plant pest defense and tea aroma quality regulation. In addition, the differences in their expression levels in different tea plant cultivars provide a possibility for the subsequent screening of tea plant resources with a specific aroma flavor. Our results deepen the understanding of terpenoid synthesis in tea plants.
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SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Ziwang Mao,Yibo Zhao,Jianhua Dong,Liangzhi Li,Yuyang Zhou
To explore novel and efficient ECL luminophores, a series of cationic iridium(III) complexes with 4,7-diphenyl-1,10-phenanthroline as the ancillary ligand and various main ligands were investigated in this work. The comprehensive studies demonstrate complex 1, with 2-phenylbenzothiazole (bt) as main ligand, exhibited superior ECL properties in aqueous buffer compared to other complexes. Importantly, the structurally optimized complex 1 was successfully further encapsulated into silica nanoparticles to prepare electrochemiluminescent iridium(III)-doped silica nanoparticles (Ir@SiO 2 NPs). These Ir@SiO 2 NPs were then utilized to construct a sensitive and specific ECL aptamer sensor for detecting acetamiprid residues in tea. Notably, our developed sensor demonstrated wide linear ranges from 10 pM to 10 μM and low limit of detection of 1.3 pM. These experimental results presented in this work undoubtedly demonstrate iridium(III)-doped silica nanoparticles as a new and efficient type of ECL nano-emitter have great potentials in ECL-related analytical applications in the future.
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FOOD CONTROL
·
2023
·
IF 6
Lingli Sun,Suwan Zhang,Qiuhua Li,Erdong Yuan,Ruohong Chen,Feiteng Yan,Xingfei Lai,Zhenbiao Zhang,Zhongzheng Chen,Qian Li,Shili Sun
Oolong tea have better richness, sweetness and throat after a certain time of storage. These variations in taste and quality are closely related to changes in the composition of oolong tea during storage, however, the metabolic profiles of oolong tea during storage have not been reported so far. Therefore, we evaluated the differences in taste quality of oolong tea in four storage years (1991, 2001, 2011 and 2021) by using electronic tongue technology, and the results showed that the bitterness, astringency and richness of oolong tea are significantly reduced during storage, but the acidity have the opposite trend, while the sweetness did not trend, with the least sweetness in 2011 and no difference in sweetness in the rest of the years. We further explored the metabolites contributing to the differences in taste quality of Dancong tea in four storage years (1991, 2001, 2011 and 2021) using metabolomics technique and the results showed that a total of 1245 metabolites were detected, of which nine were taste characteristic components of Aged Dancong tea. Dancong tea mainly increased azelaic acid, suberic acid, 2-hydroxyphenylacetic acid and other sour characteristic components in aged tea by regulating phenylalanine metabolism, metabolic pathways, and pyrimidine metabolism and mainly decreased benzamide, propyl 4-hydroxybenzoate, pyrrole-2-carboxylic acid and other bitterness and astringency characteristic components by regulating arginine and proline metabolism, flavonoid biosynthesis, biosynthesis of secondary metabolites, which enhanced the sour and umami of aged tea and reduced its bitterness and astringency. This study provides theoretical support for the quality of aged oolong tea, with a view to providing guidance for the storage of oolong tea.
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Journal of Energy Storage
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2021
·
IF 6.583
Qinghai Ma,Haitao Xi,Fang Cui,Jiajia Zhang,Peng Chen,Tieyu Cui
Exploring porous carbon (PC)-based materials from low-cost, renewable precursors has always been a challenging objective, since they meet the demands of next-generation supercapacitors and electrochemical devices. Herein, hierarchical multi-heteroatoms co-doped PC (MHPC) materials are successfully achieved by the carbonization and activation of tea waste that used as the self-template. The natural structure of the tea leaves combined with the activation strategy favor the formation of special hierarchical MHPC materials, which are constructed by ultrathin MHPC nanosheets. In addition, the heteroatomic doped carbon derived from organic compounds in tea leaves can act as an ideal precursor to fabricate the resultant heteroatomic functionalized carbon nanosheets. Such heteroatoms content of carbon nanosheets are adjustable by altering pyrolysis condition to effectively enhance the electrochemical performance of PC. After activation by KOH, the as-prepared MHPC, especially MHPC-3 (weight ratio of KOH/pre-carbonized tea waste is 3:1) has a superior supercapacitance of up to 170 and 132 F g −1 at current density of 0.5 and 10 A g −1 in KOH electrolyte, and an excellent capacitance retention of ∼94.8% over 14,000 cycles at 3 A g −1 . The result reported here provides valuable guidelines of high-performance energy storge devices from natural sources at the industrial scale.
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MOLECULES
·
2021
·Vol. 26
·期 24
·7464
·
IF 4.412
Liya Zeng,Yongchang Liu,Zhihui Yuan,Zhe Wang
The purpose of this work was to evaluate the possibility of adding tea saponin (TS) to reduce the synthetic surfactant concentration, and maintain or improve the shelf stability of nanoemulsions. TheZanthoxylum bungeanumessential oil (2.5 wt%) loaded oil-in-water nanoemulsions were co-stabilized by Tween 40 (0.5–2.5 wt%) and TS (0.1–5 wt%). A combination of several analytical techniques, such as dynamic laser scattering, interfacial tension, zeta potential, and transmission electron microscope, were used for the characterization of nanoemulsions. Low levels of TS (0.1–0.5 wt%) with Tween 40 had significant effects on the emulsification, and a nanoemulsion with the smallest droplet diameter of 89.63 ± 0.67 nm was obtained. However, in the presence of high TS concentration (0.5–5 wt%), micelles generated by the non-adsorbed surfactants in the aqueous lead to droplets growth. In addition, the combinations of Tween 40 and TS at the high level (>3.5 wt%) exerted a synergistic effect on stabilizing the nanoemulsions and preventing both Ostwald ripening and coalescence. The negative charged TS endowed the droplets with electrostatic repulsion and steric hinderance appeared to prevent flocculation and coalescence. These results would provide a potential application of natural TS in the preparation and stabilization of nanoemulsions containing essential oil.Keywords:tea saponin;Zanthoxylum bungeanum;biosurfactants;Ostwald ripening;electrostatic repulsion
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Chao Wang,Juan Li,Ya Zhang,Zhongrong He,Yin Zhang,Xingmin Zhang,Zhuoyue Guo,Jianan Huang,Zhonghua Liu
The sensory qualities , aroma profile, and microstructural features of instant Pu-erh teas (IPTs) produced by electrostatic spray drying (ESD) were evaluated by sensory and instrumental analyses and compared with those produced by other drying methods (freeze-drying [FD], vacuum drying [VD], and conventional spray drying [CSD]). The sensory qualities of ESDIPT were similar to those of FDIPT, and better than those of VDIPT and CSDIPT. Eighty-eight volatiles were detected in all IPTs, and 45 odor-active compounds were captured. Most of their OAVs were higher in ESDIPT than in VDIPT and CSDIPT but were lower than those in FDIPT. Dihydro-β-ionone had the highest OAV. Aroma recombination experiments were performed to verify the identification results. ESDIPT was present in the shape of microspheres with many regular concave surfaces, which was different from those treated by other drying methods. In terms of sensory quality and productivity, ESD would be a potential method for IPT production.
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JOURNAL OF CHROMATOGRAPHY A
·
2014
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IF 4.258
Xiaoyan Zheng,Lijun He,Yajing Duan,Xiuming Jiang,Guoqiang Xiang,Wenjie Zhao,Shusheng Zhang
New poly(ionic liquid) immobilized magnetic nanoparticles (PIL-MNPs) were synthesized via co-polymerization of 1-vinyl-3-hexylimidazolium-based ionic liquid and vinyl-modified magnetic particles and were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and magnetic measurements. The PIL-MNPs were utilized as adsorbent phases in magnetic solid-phase extraction (MSPE). The extraction and enrichment efficiency were evaluated by using four organophosphorus pesticides (parathion, fenthion, phoxim and temephos) as test analytes. Various parameters, such as amount of adsorbent, adsorption time, desorption solvent and time, and ionic strength were investigated. The proposed method showed good linearity for the analytes in the concentration range of 1–200 μg L −1 with a correlation coefficient ( R ) > 0.9963. Low limit of detection of 0.01 μg L −1 and high enrichment factors ranging from 84 to 161 were achieved. The proposed method has been successfully used to determine organophosphorus pesticides from three tea drink samples with satisfactory recovery of 81.4–112.6% and RSDs of 4.5–11.3%. The PIL-MNP adsorbent can be reused for 20 times without a noticeable decrease in extraction efficiency.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2026
·
IF 5.7
Xiaoyu Yang,Jiawei Zhang,Jinyu Yang,Junhua Wang,XiaoXiao Jiang,Jianzhi Zhao,Xiangyan Chen,Leilei Chen,Qingxin Zhou
E. cristatum drives metabolic transformation during solid-state fermentation. • Multi-omics reveals microbial mechanisms underlying AVL fermentation. • Metaproteomics identified enzymes involved in plant substrate transformation. • Fermentation reshaped non-volatile metabolites and aroma profiles of AVL. • Protein–metabolite associations reveal functional modules in fungal fermentation. Fermentation by food microorganism provides an effective approach for transforming plant-based substrates, yet the microbial mechanisms underlying these biochemical processes remain insufficiently understood. In this study, Eurotium cristatum -mediated solid-state fermentation of Apocynum venetum L. tea (AVL) was investigated at 2, 4 and 6 days using an integrated multi-omics approach and sensory-related analyses. Fermentation decreased bitterness and astringency of AVL infusions, accompanied by reductions in total polyphenols and flavonoids. Untargeted metabolomics identified 41 core differential non-volatile metabolites, predominantly fatty acyls, glycerophospholipids, steroids, phenolics, and terpenoids. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment highlighted phenylpropanoid-, flavonoid-, and isoflavonoid-related pathways. Volatile profiling identified 16 aroma-active compounds. Changes in aldehydes, ketones, alcohols, and esters contributed to fermentation-associated aroma characteristics. Metaproteomic analysis identified 4614 E. cristatum proteins, including 1838 differential proteins enriched in functional categories related to catalytic activity, oxidoreductase activity, glycosyl bond hydrolase activity, carbohydrate metabolism, and secondary metabolism. Integrated multi-omics analysis showed that non-volatile metabolite remodeling was associated with multiple microbial functional modules related to carbon metabolism, redox reactions, and secondary metabolism while fatty acid and amino acid metabolism were associated with the formation of characteristic aroma-active compounds. Overall, E. cristatum -mediated fermentation of AVL represents a growth-coupled, enzyme-associated biotransformation process in which coordinated microbial metabolic activities contribute to metabolites remodeling and the development of characteristic taste and aroma properties. These findings provide a mechanistic basis for understanding E. cristatum -mediated transformation of plant substrates and offer insights into the formation of characteristic quality attributes during fermented tea production.
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Biomaterials Advances
·
2023
·
IF 0
Jie Wang,Yiming Sun,Xiaoqing Liu,Yongyuan Kang,Wangbei Cao,Juan Ye,Changyou Gao
Both bacteria-infection and excessive inflammation delay the wound healing process and even create non-healing wound, thus it is highly desirable to endow the wound dressing with bactericidal and anti-oxidation properties. Herein an antibacterial and antioxidation hydrogel based on Carbomer 940 (CBM) and hydroxypropyl methyl cellulose (HPMC) loaded with tea polyphenols (TP) and hyperbranched poly- l -lysine (HBPL) was designed and fabricated. The hydrogel killed 99.9 % of methicillin-resistant Staphylococcus aureus ( MRSA ) and Escherichia coli ( E. coli ) at 10 7 CFU mL −1 , and showed strong antioxidation against H 2 O 2 and 2,2-di(4- tert -octylphenyl)-1-picryl-hydrazyl (DPPH) radicals without noticeable cytotoxicity in vitro . The CBM/HPMC/HBPL/TP hydrogel significantly shortened the inflammatory period of the MRSA-infected full-thickness skin wound of rats in vivo , with 2 orders of lower MRSA colonies compared with the blank control, and promoted the wound closure especially at the earlier stage. The inflammation was suppressed and the vascularization was promoted significantly as well, resulting in reduced pro-inflammatory factors including interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), and increased anti-inflammatory factors such as interleukin-4 (IL-4) and interleukin-10 (IL-10).
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