ACTA PHARMACOLOGICA SINICA
·
2022
·Vol. 44
·期 6
·1262-1276
·
IF 7.169
Song Qi-qi,Lin Li-ping,Chen Ya-li,Qian Jia-cheng,Wei Ke,Su Jian-wei,Ding Jian-hua,Lu Ming,Liu Yang,Tan Ren-xiang,Hu Gang
Malignant glioma is the most fatal, invasive brain cancer with limited treatment options. Our previous studies show that 2-(indol-3-ylmethyl)-3,3′-diindolylmethane (LTr1), a major metabolite of indole-3-carbinol (I3C) derived from cruciferous vegetables, produces anti-tumour effect against various tumour cell lines. In this study we characterized LTr1 as a novel anti-glioma agent. Based on screening 134 natural compounds and comparing the candidates’ efficacy and toxicity, LTr1 was selected as the lead compound. We showed that LTr1 potently inhibited the viability of human glioma cell lines (SHG-44, U87, and U251) with IC 50 values of 1.97, 1.84, and 2.03 μM, respectively. Furthermore, administration of LTr1 (100,300 mg· kg −1 ·d −1 , i.g. for 18 days) dose-dependently suppressed the tumour growth in a U87 xenograft nude mouse model. We demonstrated that LTr1 directly bound with TrkA to inhibit its kinase activity and the downstream PI3K/AKT pathway thus inducing significant S-phase cell cycle arrest and apoptosis in SHG-44 and U87 cells by activating the mitochondrial pathway and inducing the production of reactive oxygen species (ROS). Importantly, LTr1 could cross the blood-brain barrier to achieve the therapeutic concentration in the brain. Taken together, LTr1 is a safe and promising therapeutic agent against glioma through inhibiting TrkA/PI3K/AKT pathway.
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高影响因子
Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Gao De,Han Feng,Waterhouse Geoffrey I. N.,Li Yan,Zhang Lili
A series of FeTPP-N/C-X catalysts were successfully prepared by adsorption of Fe tetraphenylporphine (FeTPP) onto a porous nitrogen-doped carbon (N/C) support using an ultrasonication method. The FeTPP-N/C catalysts were then used for the preparation of 5-hydroxymethyl-2-furan acid (HMFCA) by catalytic oxidation of 5-hydroxymethylfurfural (HMF) at 80 °C. The effects of reaction temperature, NaOH dosage, reaction time, and oxygen pressure on HMF conversion and HMFCA yield were systematically investigated. Under optimized testing conditions, a FeTPP-N/C-1 catalyst prepared using equal masses of FeTPP and N/C delivered a HMF conversion in water of 92.4%, with a selectivity to HMFCA of 97.2% over 6 h at an oxygen pressure of 1.0 MPa. Further, the catalyst showed good stability in five consecutive HMF oxidation tests. Based on the catalytic tests, and detailed catalyst characterization studies before and after reaction, a probable reaction mechanism was proposed for the selective oxidation of HMF to HMFCA by the FeTPP-N/C catalysts. Graphical Abstract
A series of FeTPP-N/C
catalysts were successfully prepared by the adsorption of iron
tetraphenylporphine (FeTPP) onto a porous nitrogen-doped carbon (N/C) support
using an ultrasonication method. The FeTPP-N/C catalysts were then used for the
catalytic aerobic oxidation of HMF to HMFCA. Under the optimized reaction conditions (temperature
80 °C, reaction time 6 h, O 2 pressure 1 MPa), a high HMF conversion
of 92.4% and a remarkable HMFCA selectivity of 97.2% were obtained, suggesting
that the developed noble-metal free catalyst has excellent promise for the
synthesis of valuable HMFCA from HMF.
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 44
·20641-20653
·
IF 4.682
Ren Meiqi,Dong Xiaoli,Wang Yu,Zheng Nan,Ma Hongchao,Zhang Xiufang
Conjugated microporous polymers have attracted significant attention in recent years due to their high porosity, broad functionality and excellent chemical stability. In this work, the small-sized layered structure of poly(phenylenethiazolo[5,4-d]thiazole) (p-PhTT) copolymer was achieved by a facile solvothermal-assisted exfoliation process in a polar organic solvent. Owing to the shorter diffusion length, the layered structure could enhance carrier diffusion from the interior to the surface, as well as provide more active sites in photocatalytic reaction. The obtained exfoliated p-PhTT copolymers show good dispersion and stability in aqueous solution and exhibit efficiently photocatalytic activity for degrading rhodamine B dye under visible-light irradiation. This effective method offers a promising strategy for exfoliating other organic porous polymers. Graphical Abstract
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Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-13
·
IF 6.352
Zhong Zhi-Hao,Zhang Yu-Qing
Background The immobilized technology for microbial or cells has the advantages of high microbial activity, high microbial density per unit space, good tolerance, strong shock, load resistance, high processing efficiency, and high reuse rate. It is now widely used in environmental remediation, water quality treatment, biodegradation, food industry, chemical analysis, energy development, medicine and pharmaceuticals, and other fields.Results A novel Escherichia coli cell-immobilizing polyvinyl alcohol hydrogel membrane (ECI-PVAHM) was prepared by unidirectional nanopore dehydration (UND) from a 10% polyvinyl alcohol (PVA) aqueous solution containing enhanced green fluorescent protein-labeled E. coli. This bacteria-loaded film has high water stability, flexibility, transparency, and mechanical robustness. Its tensile strength, elongation rate, and swelling rate are in the ranges 0.66–0.90 MPa, 300–390%, and 330–800%, respectively. The effective bacterial load of ECI-PVAHM is 2.375 × 109–1010 CFU/g (dry weight), which does not affect the original crystal structure of the PVAHM. This biofilm has a porous network structure with pore sizes between 0.2 and 1.0 μm, and these cells are embedded in the PVAHM network. When the immobilized cells were continuously cultured for 20 days, and the medium was renewed twice daily, their relative proliferation efficiency after 40 cycles could still be maintained at ~ 91%.Conclusion The above results show that the cell division, proliferation ability, and metabolic activity of immobilized E. coli were not affected by the physical barrier of the porous network structure of the hydrogel. This UND-based ECI-PVAHM has potential applications in molecular biology, biopharmaceutical expression and production, bioreactors, and fuel cells.
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FOOD SCIENCE AND BIOTECHNOLOGY
·
2022
·Vol. 32
·期 3
·361-369
·
IF 3.231
Su Anxiang,Ma Gaoxing,Ma Ning,Pei Fei,Yang Wenjian,Hu Qiuhui
Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear. Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites. FVP were used to vitro fermentation through human fecal inoculums. In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota. The metabolic profiles were investigated using untargeted metabolomics approaches in the LC-MS platform. The results showed that FVP was mainly consisted of glucose, mannose, xylose, fucose and galactose. FVP is shown to increase the relative abundances of Bifidobacteriaceae , as well as Bacteroidaceae and remarkably decrease the numbers of genera Lachnospiraceae coupled with Enterococcaceae . The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids. FVP may exhibit biological activity function by regulating gut microflora composition and metabolites.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-11
·
IF 6.408
Zhou Hang-Yu,Peng Li-Jing,Tian Tao,Zhang Wei-Yi,Chen Guo-Ying,Zhang Hao,Yang Feng-Qing
An effective, selective, and multicolor colorimetric assay for Cu 2+ detection based on the regulation of peroxidase-like nanozyme-mediated etching of gold nanorods (Au NRs) is proposed. Cu 2+ -creatinine complex is selected as the nanozyme that exhibits excellent peroxidase-like activity even in the case of low concentration of Cu 2+ , which can catalyze 3,3,5,5-tetramethylbenzidine (TMB) to produce oxidized TMB (TMB + ) in the presence of hydrogen peroxide, and TMB + is oxidized to generate TMB 2+ after adding H + , and the TMB 2+ can etch Au NRs. The determination of Cu 2+ is achieved based on the blue shift of the longitudinal localized surface plasmon resonance peak of Au NRs. Under the optimal conditions, the developed colorimetric assay exhibits high sensitivity for the detection of Cu 2+ (limit of detection is 0.034 μM) with a wide linear range of 0.05–4.0 μM ( R 2 = 0.987). The solution shows a rainbow-like color in response to the increase of Cu 2+ concentration, which can realize the semi-quantitative detection of Cu 2+ by naked eyes. In addition, the developed method exhibits excellent selectivity for Cu 2+ -detection. The established method was used for the determination of Cu 2+ in lake water, soil, and normal human serum with satisfactory recovery of spiked samples. Graphical abstract
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 38
·期 6
·1349-1355
·
IF 2.726
Xiao Meng,Qi Wenqian,Jia Shuanghui,Pang Mengting,Shi Fengcheng,Mao Hui
Significant concerns have been raised over the removal of antibiotics, such as tetracyclines(TC) in aquatic environments. Herein, we synthesized a new type of heterogeneous catalyst by supporting Fe 0 nanopartciles(FeNPs) onto carbon coated ZIF-8 (C@ZIF-8). The carbon layer formed by glucose was beneficial to maintain the morphology and porous structure of ZIF-8, which can also appropriately improve the hydrophobicity of ZIF-8 for enriching the TC. The as-prepared FeNPs-C@ZIF-8 catalyst featured an extreme large specific surface area(1122.16 m 2 /g), and the supported FeNPs with an average diameter of 6.13 nm exhibited a high dispersity on the supporting matrix of C@ZIF-8. For the removal of tetracycline, the large specific surface area of FeNPs-C@ZIF-8 allowed for an easy access of tetracycline to the FeNPs, while the highly dispersed FeNPs served as actived sites for the efficient degradation of tetracycline. A synergistic effect between adsorption and catalytic degradation of FeNPs(5%, mass fraction)-C@ZIF-8 was proven to be responsible for the high-performance removal of tetracycline with the removal efficiency high up to 93.02% at pH 5, 25 °C. The FeNPs-C@ZIF-8 was capable of recycling after activation with supplementary Fe 0 , which still maintained a high removal efficiency of 75.52% in the 5th cycle within 20 min.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-12
·
IF 17.694
Ma Yuzhu,Zhang Hongjin,Lin Runfeng,Ai Yan,Lan Kun,Duan Linlin,Chen Wenyao,Duan Xuezhi,Ma Bing,Wang Changyao,Li Xiaomin,Zhao Dongyuan
Multi-chambered architectures have attracted much attention due to the ability to establish multifunctional partitions in different chambers, but manipulating the chamber numbers and coupling multi-functionality within the multi-chambered mesoporous nanoparticle remains a challenge. Herein, we propose a nanodroplet remodeling strategy for the synthesis of hierarchical multi-chambered mesoporous silica nanoparticles with tunable architectures. Typically, the dual-chambered nanoparticles with a high surface area of ~469 m 2 g −1 present two interconnected cavities like a calabash. Furthermore, based on this nanodroplet remodeling strategy, multiple species (magnetic, catalytic, optic, etc.) can be separately anchored in different chamber without obvious mutual-crosstalk. We design a dual-chambered mesoporous nanoreactors with spatial isolation of Au and Pd active-sites for the cascade synthesis of 2-phenylindole from 1-nitro-2-(phenylethynyl)benzene. Due to the efficient mass transfer of reactants and intermediates in the dual-chambered structure, the selectivity of the target product reaches to ~76.5%, far exceeding that of single-chambered nanoreactors (~41.3%).
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NEUROCHEMICAL RESEARCH
·
2022
·Vol. 48
·期 2
·570-578
·
IF 4.414
Guo Shenglong,Lei Qi,Guo Hena,Yang Qian,Xue Yanli,Chen Ruili
Ferroptosis and neuroinflammation play a crucial role in the pathogenesis of Alzheimer’s disease (AD), and Edaravone (EDA) has been demonstrated to have anti-inflammatory, antioxidant and neuroprotective effects in neurodegenerative diseases. However, the relationship between EDA and ferroptosis in AD is unidentified. This research aimed to elucidate the mechanism of EDA in AD with Aβ 1-42-induced HT22 cells as in vitro cell model. The results showed that EDA could significantly reduce Aβ 1-42 -induced apoptosis of HT22 cells and formation of pro-inflammatory factors TNF-α, IL-1β and IL-6, prevent the activation of TLR4/NF-κB /NLRP3 signaling pathway, and inhibit ferroptosis and lipid peroxidation. Taken together, EDA contributes to inhibiting neuroinflammatory injury and ferroptosis in Aβ 1-42-induced HT22 cells, and thus may be a potential candidate for the treatment of AD.
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Food Analytical Methods
·
2023
·Vol. 16
·期 4
·709-720
·
IF 2.9
Zhou Binbin,Sheng Xingxin,Xie Hao,Zhou Sisi,Huang Lijun,Zhang Zuokun,Zhu Yongbo,Zhong Ming
Nitrofurazone (NF) is a kind of synthetic antibiotic with teratogenic and carcinogenic properties, which is often residual in animal food, so it is of great significance to detect it with high selectivity and rapid. In this study, a molecularly imprinted electrochemical sensor was constructed by electropolymerization for the high-specificity, high sensitivity,and rapid detection of NF in milk. By sequentially modifying reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) on the glassy carbon electrode, the effective surface area and electron transfer ability of the electrode are improved, the double amplification of the electrical signal is realized, and the sensitivity and stability of the sensor are improved. A molecularly imprinted polymer (MIP) is polymerized on the surface of the modified electrode by using NF as a target molecule and o -phenylenediamine ( o –PD) as a functional monomer to improve the specific detection ability of the sensor for NF. The experimental conditions (the number of turns of depositing RGO, the thickness of the MIP, the elution time, and the adsorption time) were optimized to attain the best detection performance of the sensor. Differential pulse voltammetry (DPV) was used to detect NF by redox probe ([Fe(CN) 6 ] 3−/4− ). The constructed sensor exhibited an admirable linear relationship between 5 and 1000 nmol L −1 ( R 2 = 0.9968) and the detection limit was 0.18 nmol L −1 (S/N = 3). Compared to the non-imprinted sensor, the imprinting factor was found to be 3.09. In addition, the sensor also showed excellent repeatability (RSD = 1.17%) and stability, and also showed a good recovery rate in actual sample detection (99.06–101.46%), which is expected to be applied to the detection of other harmful substances in food.
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JOURNAL OF ELECTRONIC MATERIALS
·
2023
·Vol. 52
·期 4
·2794-2806
·
IF 2.1
Li Shengnan,Meng Haoyan,Fan Wentao,Shen Junqing,Xu Shengang,Liu Yingliang,Cao Shaokui
Abstract Woven resistive random access memory (RRAM) is a promising subject in flexible wearable electronic devices. In this work, polyvinylammonium iodate (PVAm·HI) is added to the FAPbI 3 perovskite precursor solution to fabricate a flexible fibrous woven RRAM array by improving the flexibility and stability of FAPbI 3 perovskite. The long carbochain polymer backbone of PVAm serves as nucleation sites and a growth template to improve the perovskite crystallization process, and to in situ crosslink the perovskite grains at the crystal interface. The functional Al@FAPbI 3 :PVAm fibers are then prepared by solution dip-coating to assemble the fibrous crosspoint RRAM device with the structure of Al@FAPbI 3 :PVAm/Al by crossing bare aluminum (Al) fibers with functional fibers. Compared to a pristine device, the PVAm-modified devices exhibit a considerable improvement in the ON/OFF ratio and environmental stability. Specifically, the 2% PVAm-modified FAPbI 3 RRAM devices show an evident bipolar resistive switching (RS) behavior with a high ON/OFF ratio of 10 9 , which can easily achieve multilevel storage by setting the compliance current ( I cc ) to increase the storage density in the crosspoint array. Interestingly, a 100-times bent plastics-encapsulated device still exhibits a good RS behavior. This work provides a facile approach to achieve high-density storage in flexible wearable electronic devices.
Graphical Abstract
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POLYMER BULLETIN
·
2023
·Vol. 80
·期 12
·13319-13341
·
IF 3.2
Zhang Qunli,Zhai Weili,Cui Linlin,Liu Yupeng,Xie Wenjing,Yu Qimeng,Luo Hao
Polylactic acid (PLA)/starch acetate (SA) base film was prepared by scraping film method with PLA as the base film material, modified SA as the filling phase and polyethylene glycol (PEG) as plasticizer. Chitosan (CS)/tea polyphenol (TP) antibacterial agent was coated on the surface of PLA/SA base film by coating method. The morphology and structure of the composite film were characterized by FTIR, XRD, SEM and thermogravimetric analysis, and the mechanical, optical and antibacterial properties were tested and analyzed. The results showed that CS/TP compound antibacterial agent was well combined with PLA/SA base film. Compared with the PLA/SA base film, when the content of TP (based on the mass of PLA/SA base film, the same below) was 0.4%, the tensile strength of the composite film increased by 29.33%, had good mechanical strength (24.96 ± 0.65 MPa), and the elongation at break increased by 29.34%; There was no obvious change in transparency, the light transmittance decreased by 0.86%; At the same time, the antibacterial composite film had good antibacterial effect on Escherichia coli , Staphylococcus aureus and Bacillus subtilis . PLA/SA/CS/TP antibacterial composite film could be used in food packaging, especially those designed to protect food from light and microbial decay.
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