BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Ganqiao Ran,Dan Tan,Jiping Zhao,Fan Fan,Qiang Zhang,Xingjuan Wu,Peiyao Fan,Xinlei Fang,Xiaoyun Lu
d -Allulose is a promising low-calorie sweetener especially for diabetes and obesity patients. The functionalized polyhydroxyalkanoate (PHA) nano-beads decorated with d -tagatose 3-epimerase (DTE) was produced in recombinant endotoxin-free ClearColi , whereby the expression, purification, and immobilization of the active DTE were efficiently combined into one step. The immobilized DTE exhibited remarkable enzyme activity of 649.3 U/g beads and extremely high stability at a harsh working condition (pH 7.0–8.0, 65 °C). When DTE-PHA beads were subjected to enzymatic synthesis of d -allulose, a maximum conversion rate of 33% can be achieved at pH 7.0 and 65 °C for 3 h, and DTE-PHA beads retained about 80% of its initial activity after 8 continuous cycles. Moreover, the d -allulose/ d -fructose binary mixture can be simply separated by a single cation exchange resin-equipped chromatography. Taken together, DTE-PHA beads are promising and robust nano-biocatalysts that will remarkably simplify the production procedures of d -allulose, contributing to its cost-effective production.
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高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Lihong Qiao,Tingting Sun,Xiaohua Zheng,Min Zheng,Zhigang Xie
Targeting imaging to the desired site of action can increase the accuracy and effectiveness of diagnostic and treatment. In this work, a series of fluorescent carbon dots (CDs) were prepared by varying molar ratios of d -glucose (Glu) to l -aspartic acid (Asp). Their photophysical properties, morphologies and structures were investigated in detail. More important, the targeting ability was screened by confocal laser scanning microscopy and flow cytometry. The results indicate that CDs prepared from the optimal molar ratio of Glu/Asp (7:3) exhibit the highest targeting ability on C6 glioma cells. This work highlights the interplay of molecular design and corresponding functions, and open new possibility of developing state-of-art nanoparticles for biomedical applications.
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高影响因子
Nanoscale
·
2017
·Vol. 10
·期 4
·1631-1640
·
IF 7.367
Lingcan Kong,Xuefeng Chu,Chuanxi Wang,Hong Zhou,Yukang Wu,Wenwei Liu
The aggregation-induced emission (AIE) behavior of metal nanoclusters has attracted much attention owing to their extensive application prospects in bio-imaging and chemical sensors. However, the intrinsic mechanism of metal nanoclusters’ aggregation-induced emission is still not very clear. Herein, Cu nanoclusters S1 and Ag(I)-doped Cu/Ag nanoclusters S2 and S3 coated with D-penicillamine are designed and synthesized by a self-assembly strategy. S1–S3 show strong luminescence properties with luminescence quantum yields as high as 11.4%–14.2%. Moreover, their luminescence peak position shows an obvious hypsochromic shift from 615 (S1) to 570 nm (S3). With the introduction of Ag(I) ions, the assembly morphology also shows clear changes from the irregular assembly structure (S1) to large spherical particles with the average size of 0.18 μm for S2 and 0.47 μm for S3. A detailed investigation of high-resolution transmission electron microscopy (HRTEM) images, X-ray diffraction (XRD) patterns, ESI-TOF-mass spectra, UV-vis absorption spectra, photoluminescence spectra and luminescence lifetimes of Cu/Ag nanoclusters S2 and S3 indicates that Cu/Ag nanoclusters are actually a Cu/Ag alloy nanocluster superstructure and the microscopic arrangement of S2 and S3 is more compact and ordered relative to S1. The more compact and ordered Cu/Ag alloy nanocluster superstructure enhances the metal–metal interaction of inter-nanoclusters and intra-nanoclusters and facilitates the radiative transition of ligand-to-metal charge transfer (LMCT) and/or ligand-to-metal–metal charge transfer (LMMCT), which results in the aggregation-induced emission phenomenon. In addition, an enhanced metal–metal interaction increases the average metal–metal distance of the Cu/Ag nanocluster superstructure, leading to a hypsochromic shift of emission spectra. Furthermore, the Cu/Ag alloy nanoclusters show good stability and reversibility on pH cycling between pH = 3 and 7 and temperature cycling between 5 °C and 48 °C. The Cu/Ag alloy nanoclusters can be used as probes for Ag(I) ion and halide anion detection in real water samples by the ratiometric PL method (I570/I615).
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高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2017
·Vol. 44
·期 1
·491-502
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IF 1.369
Li Shaoxin,Zhao Bing,Kan Wei,Wang Liyan,Song Bo,Chen Shuwen
A novel pH fluorescence probe (PIF), derived from phenanthro[9,10- d ]imidazole bound to the fluorescein, is designed and synthesized based on the intramolecular hydrogen bond and then its spectra properties are investigated. Probe PIF exhibits negligible fluorescence intensity at acidic pH condition due to ESIPT quenching caused by the formation of the intramolecular hydrogen bonds, and a significant fluorescence enhancement in neutral and weak alkaline environments because of the strengthened ICT process arose from the fracture of the intramolecular hydrogen bonds. The studies of UV–Vis and fluorescence spectra mean that probe PIF is able to respond linearly and reversibly over pH from 6.5 to 9.0 with p K
a of 7.0. Moreover, the pH responses of probe PIF are stable and not disturbed by the common cations and anions. The possible response mechanism has been proposed according to the analysis of 1 H NMR and DFT calculations.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Zhen He,Xiangyun Tan,Ming Yuan,Liang Chen,Yan Meng,Qi Wang,Junjie Hu,Zhenpeng Qiu,Yuan Yang
Acute liver injury (ALI) is a prevalent form of hepatic disease associated with significant morbidity and mortality due to medical treatments, exposure to toxins or viral infections. Anethole trithione (ATT) is a heterocyclic sulfur compound recognized for its chemoprotective properties against cancer and drug-induced toxicity. This study aimed to evaluate the effectiveness of ATT in the treatment of ALI. The therapeutic effects of ATT on hepatic injury were evaluated in vivo by inducing ALI in mice through the administration of lipopolysaccharide (LPS) and D-galactosamine (D-Gal). Additionally, HepG2 and Huh7 cells exposed to LPS were utilized to investigate the underlying mechanisms in vitro. The results indicated that ATT significantly reduced the production of reactive oxygen species (ROS), mitigated oxidative stress-related biochemical markers, and inhibited hepatocyte apoptosis in vivo, resulting in marked improvement in ALI in the murine model. Mechanistic studies conducted both in vivo and in vitro demonstrated that ATT alleviates LPS/D-Gal-induced ALI by inhibiting ROS production and the activity of nuclear factor-kappa B (NF-κB). Collectively, these findings underscore the potential therapeutic benefits of ATT in the management of ALI.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Boliang Li,Binfei Li,Liping Du,Teng Lu,Guolin Shao,Zhaomin Li,Mengyuan Zhang,Junhao Zhang
High carbon emissions and low efficiency in thermal utilization are pivotal challenges in heavy oil development. Addressing the urgent need to enhance heavy oil production, this paper introduces an innovative approach in which aerogel and flue gas serve as supplementary additives in steam thermal recovery. To investigate the mechanisms behind the improved heat transfer and oil recovery facilitated by the combination of aerogel and flue gas, condensation heat transfer experiments and 2-D visual oil displacement experiments were carried out. The condensation heat transfer coefficient, oil displacement performance, and heat utilization efficiency were analyzed and compared. The results showed the porous structure of the aerogel enhances its thermal insulation properties by adsorbing flue gas, thereby reducing the steam-to-cold object condensation heat transfer coefficient by 60.6%. The cementation between aerogel nanoparticles (NPs) retained in the formation and rock particles formed an insulating layer that impedes the upward propagation of steam heat. Combined with efficacy of flue gas foam in improving fluid flow, this promoted the horizontal expansion of the steam chamber, resulting in an 8.2% and 3.4% increase in the steam chamber’s extent compared to using steam and a combination of steam with aerogels, respectively. Moreover, the profile control and thermal insulation impacted following the NPs, lowering the water cut in the produced fluid and enhancing the oil recovery by 7.9%. Upon the application of flue gas, this enhancement in recovery extended to 9.3%.
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高影响因子
FUEL
·
2025
·
IF 7.5
Yanan Ma,Dingyu Zhang,Haimeng Wang,Yuxuan Liang,Yuan Zhang,Fengfeng Yan,Liang Sun,Qian Wang,Wei Li
Synthesis of multiple-site single-atom catalyst (SAC) with metal-containing nanoparticles by using zeolite imidazole framework (ZIF) for improving the bifunctional electrocatalytic performance is of great significance, yet it is also a challenge. Trimetallic ZIF-derived porous carbon nanosheets with the CoFe alloy nanoparticles enclosed in nitrogen-doped carbon nanotubes (N-CNTs) are fabricated by utilizing three transition metals as ZIF precursors and sodium chloride as templates via a typical two-step process, including synthesis and pyrolysis. By building CoFe alloy and tailoring d-band center ( E d ) of the Co site, the as-obtained FeCoZn@S-CN ZIF exhibits predominant kinetic activity and durability towards ORR and OER with an extremely low potential gap (Δ E ) value of 0.584 V, exceeding many other efficient counterparts. This study provides an effective approach for designing and synthesizing atomically dispersed multi-metal site catalysts with alloyed nanoparticles to achieve high-efficient bifunctional electrocatalytic performance in the application of the recharged zinc-air battery (ZAB).
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APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Xiao Shang,Kai-Li Yan,Zi-Zhang Liu,Shan-Shan Lu,Bin Dong,Jing-Qi Chi,Xiao Li,Yan-Ru Liu,Yong-Ming Chai,Chen-Guang Liu
Oxidized carbon fiber (oCF) as support successfully realizes the vertical growth of uniform WS 2 nanosheets arrays for efficient hydrogen evolution reaction (HER) via a facile hydrothermal process. Thanks to oxygen functional groups on oCF, vertical WS 2 nanosheets structures have grown more easily on oCF, which can provide better dispersion, short charge transfer distance and more exposed active sites for HER in comparison with bulk WS 2 and WS 2 nanosheets on bare carbon fiber (CF) fabricated at the same condition. The electrochemical measurements confirmed that WS 2 /oCF possesses better HER activity than bulk WS 2 and WS 2 /CF. Especially, the 10-h stability with unchanged vertical WS 2 nanosheets morphology further demonstrate the positive effect of oxygen functional groups on the enhanced vertical structure and close combination between WS 2 and oCF. It may offer a facile way to realize more exposed active sites from stable electrocatalyst hybrids of transition metal sulfides by surface oxidization of carbon supports.
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高影响因子
ChemCatChem
·
2016
·Vol. 9
·期 3
·458-464
·
IF 4.724
Ting Yang,Lan-Lan Lou,Wenjun Yu,Yajun Feng,Hao Li,Kai Yu,Shuangxi Liu
Graphical Porous but extraordinarily durable : 3 D ordered macroporous alumina–carbon composite supported Pt nanocatalysts are applied in the asymmetric hydrogenation of ethyl pyruvate. They exhibit high catalytic activity and extraordinary durability. The three-dimensionally ordered macroporous alumina-carbon composite materials were firstly prepared through a surfactant-assisted colloidal crystal template method and applied to support Pt nanoparticles. The results of SEM, XRD, TEM, and N 2 sorption analyses revealed that these composite materials possessed highly ordered macroporous structures with meso- and micropores within the skeletons of macropores and that the Pt nanoparticles were well dispersed on these materials with a very narrow size distribution. These prepared Pt nanocatalysts were evaluated in the asymmetric hydrogenation of ethyl pyruvate after chiral modification with cinchonidine. Excellent catalytic activity and enantioselectivity were achieved. More importantly, these catalysts exhibited extraordinary stability and reusability during the reaction and could be reused at least 26 times without significant loss in catalytic activity and enantioselectivity.
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高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2017
·
IF 6.035
Xuehua Ruan,Xinfang Zhang,Xuhang Liao,Xiaobin Jiang,Yan Dai,Xiaoming Yan,Gaohong He
Zeolitic imidazolate framework-8 (ZIF-8) with regular pore size (~ 0.34 nm) is widely attempted for gas separation membranes based on size sieving. However, the prospect is subjected to fabricating the continuous ZIF-8 membranes with high mechanical stability and uniformity. In response, an adhesive layer of Zn(II)-doped polydopamine (Zn-PDA) is pre-coated on the stainless steel nets in this work to improve ZIF-8 nucleation and growth. Zn-PDA layer shows high tolerance to ultrasonic agitation and heated methanol (system for ZIF-8 synthesis), little dissolution within 24 h, which supplies a high stable surface for ZIF-8 growth. Furthermore, the Zn(II) doped in PDA layer is opportunely serving as the even-distributed ZIF-8 nucleation starting sites, verified with the EDX and FE-SEM images for the aggregated Zn(II) clusters and the nascent ZIF-8 crystals. Therefore, an indented and hinged boundary is constructed between ZIF-8 and substrate to enhance the mechanical stability. The FE-SEM images after ultrasonic agitation show that the continuous ZIF-8 membranes prepared on Zn-PDA layers can tolerate the deformation to a certain extent. Finally, the prepared ZIF-8 membranes behave excellently with α H2/CO2 = 9.9, α H2/CO = 17.2, α H2/CH4 = 26.0, and permeation rate J H2 = 3980 GPU. This work supplied a useful approach to enhance mechanical stability and uniformity of defect-free ZIF-8 membranes.
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高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Deng Jun,Wu Sai,Yao Mengyun,Gao Changyou
Chirality is one of the ubiquitous phenomena in biological systems. The left handed (L-) amino acids and right handed (D-) sugars are normally found in proteins and in RNAs and DNAs, respectively. The effect of chiral surfaces at the nanoscale on cellular uptake has, however, not been explored. This study reveals for the first time the molecular chirality on gold nanoparticles (AuNPs) functions as a direct regulator for cellular uptake. Monolayers of 2-mercaptoacetyl-L(D)-valine (L(D)-MAV) and poly(acryloyl-L(D)-valine (L(D)-PAV) chiral molecules were formed on AuNPs surface, respectively. The internalized amount of PAV-AuNPs was several times larger than that of MAV-AuNPs by A549 and HepG2 cells, regardless of the chirality difference. However, the D-PAV-AuNPs were internalized with significantly larger amount than the L-PAV-AuNPs. This chirality-dependent uptake effect is likely attributed to the preferable interaction between the L-phospholipid-based cell membrane and the D-enantiomers.
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高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2016
·
IF 3.299
Xi Zhang,Jiawei Wang,Shaofeng Chen,Zongbi Bao,Huabin Xing,Zhiguo Zhang,Baogen Su,Qiwei Yang,Yiwen Yang,Qilong Ren
Preparation of a novel hybrid boron-selective adsorbent with N- methyl- d -glucamine functional groups was reported. The adsorbent was synthesized by inverse suspension polymerization method based on chemistry of sol-gel reaction. The structure and morphology of the hybrid adsorbent were characterized by MS, FTIR, XPS, BET analysis and PXRD , etc. The perfectly spherical hybrid adsorbent exhibits exceptionally efficient adsorption of boric acid from the aqueous solution. The boron adsorption behavior on the adsorbent was investigated by varying the dosages of the adsorbent, pH, and competitive ions. The adsorption isotherm was in better accordance with the Langmuir model, exhibiting a theoretical maximum adsorption capacity of 2.02 mmol/g. The adsorption capacity peaks at pH ∼ 9 due to the vast formation of stable tetrahedral structure between dominant B(OH) 4 − and the adsorbent. The presence of Na + ions has slight effect on boron adsorption while Mg 2+ cations significantly enhanced the adsorption capacity because of the simultaneous adsorption attributed by Mg(OH) 2 .
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