Frontiers in Bioengineering and Biotechnology
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2022
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IF 6.064
Yang Yiling,Wang Wenbin,Liu Kefeng,Zhao Jie
Senescence is a major inductive factor of aging-related diseases in connection with an accumulation of reactive oxygen species (ROS). Therefore, it is important to maintain ROS at an appropriate level to keep homeostasis in organisms. Superoxide dismutase (SOD) is a vital enzyme in defending against oxidative damage in vivo. Because of the defects in the direct application of SOD and SOD mimics, mounting delivery systems have been developed for the efficient applications of SOD to realize antioxidant treatment. Among these systems, mesoporous silica nanoparticles (MSNs) have been widely studied because of various advantages such as desirable stability, low toxicity, and adjustable particle sizes. Herein, SOD was immobilized on MSNs using a physical absorption strategy to construct the nanosystem SOD@MSN. The nematode Caenorhabditis elegans (C. elegans) was selected as the model organism for the subsequent antioxidant and anti-aging studies. The research results suggested the nanosystem could not only be effectively internalized by C. elegans but could also protect the nematode against external stress, thus extending the lifespan and healthspan of C. elegans. Therefore, SOD@MSN could be applied as a promising medicine in anti-aging therapeutics.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Xiaoyuan Ma,Shan Xu,Liangyu Li,Zhouping Wang
Here, an aptamer-based SERS method for the detection of Staphylococcus aureus ( S. aureus ) without immobilization using [email protected] NPs/slide as enhanced substrate was constructed. ROX-aptamer of S. aureus was modified on the surface of [email protected] NPs/slide through electrostatic interaction. Based on the specific binding effect of the aptamer to S. aureus , the ROX-aptamer fell off from the surface of the substrate, resulting in a decrease of the SERS signal intensity of the substrate. Under the optimal experimental conditions, a good linear relationship was found between SERS intensity at 1500 cm −1 and the logarithm of concentration of S. aureus in the range of 10 2 cfu/mL-10 7 cfu/mL (y = 6623-796lgx, R 2 = 0.994) with a detection limit of 6 cfu/mL. The selectivity analysis revealed that the method had higher selectivity toward the corresponding target. The results for milk sample using the developed SERS method for the detection of S. aureus were similar to those of the plate counting method. The recovery ratio was from 90.60% to 107.26%, indicating the accuracy and reliability of the developed method. This method eliminates the need for bacterial immobilization and improves the convenience and efficiency of detection.
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CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Guifang Wang,Guangchuan Liang,Huizhen Xiao,Jun Qiu,Haiyan Liu,Shaojian Ma,Sridhar Komarneni
The conventional environmental remediation materials, which are applied for the long-term treatment of mine tailings are easy to fail when affected by acid rain. Therefore, dynamic column simulation experiments were conducted to compare the immobilization persistence of As, Mn, Pb and Zn by the addition of triethylenetetramine modified montmorillonite (TTA-Mt) and calcium-based montmorillonite (Ca-Mt) in sulfide tailings during a simulated 5-year study of acid rain leaching. The structural and morphological characteristics of TTA-Mt were analyzed with FTIR, XRD, SEM, TEM and EDS. Results showed that the additions of TTA-Mt could persistently immobilize the heavy metal ions while Ca-Mt additions had little effect. After long-term simulated leaching for 5 years, TTA-Mt still had superior environmental stability for Zn and As with the immobilization rates of 96% and 86%, and the leaching amounts of Mn and Pb were less than 4.23 and 0.66 mg/kg, respectively. Morphology and chemical fraction analyses confirmed that the immobilization was mainly due to the formation of relatively stable phases by complexation and precipitation. Results presented here demonstrated the significant potential of TTA-Mt for long-term immobilization of heavy metals in tailings against acid rain and thus could provide guidance for remediation of tailings.
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ANALYTICA CHIMICA ACTA
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2023
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IF 6.2
Wenchao Geng,Linsheng Xue,Yuling Li,Jiangying Ji,Xinxin Yuan,Lihua Ding,Ruiying Yang
Herein, a novel silver ion-loaded gold microemulsion assemblies (Au/Ag + MAs) mediated multifunctional signal amplification strategy was proposed to construct a sensitive immobilization-free photoelectrochemical (PEC)/colorimetric biosensor for carcinoembryonic antigen (CEA) detection. Through the sandwiched reaction among CEA, the CEA aptamer (DNA1) loaded on the Au nanoparticles (NPs) functionalized iron oxide (Fe 3 O 4 ) nanospheres and another CEA aptamer (DNA2) immobilized on Au/Ag + MAs , a complex is formed and acquired by magnetic separation. Then, Au/Ag + MAs of the complex are disassembled into Au NPs and Ag + ions driven by an acetone response, and the obtained demulsification solution is transferred to the cadmium sulfide/cadmium telluride (CdS/CdTe) photoactive composites modified electrode . Based on the multiple inhibition functions (blocking effect of oleylamine; energy transfer effect of Au NPs; and electron snatching effect of Ag + ), the photocurrent of the electrode decreases obviously, resulting in the ultrasensitive detection of CEA (a detection limit of 16 fg mL −1 ). Interestingly, the ion-exchange reactions between CdS/CdTe composites and Ag + ions generate silver sulfide/silver telluride (Ag 2 S/Ag 2 Te) composites, and a color change of composites can be distinguished directly, leading to a quick visual detection of CEA. Compared with the traditional single-modal assay for CEA, such dual-modal PEC/colorimetric assay is a more accurate and reliable due to different mechanisms and independent signal conversion. This work will offer a new perspective for the applications of various self-assemblies in PEC bioanalysis .
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ACS Applied Materials & Interfaces
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2023
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IF 9.5
Chenhui Yan,Junyu Li,Chuan Tan,Guangyuan Chen,Qian Zhao,Yuxuan Chen,Pan He,Yulong Luo,Tao Duan,Jiehong Lei,Lin Zhu
The efficient capture and immobilization of radioiodine (I–, IO3–) is of great importance in radioactive waste management. Here, a Cu2O-loaded three-dimensional bulk cationic hydrogel composite (Cu2O@CH) was successfully prepared by simple redox reactions and UV photopolymerization, which realized the rapid enrichment and efficient immobilization of I– and IO3–. The adsorption experiments showed that the maximum adsorption capacity of Cu2O@CH for I– in the solution at pH = 3 reached 416.5 mg/g, and the adsorption capacity of IO3– in the solution at pH = 6 could reach 313.4 mg/g. It exhibited extremely fast adsorption kinetics for I– and IO3–. In addition, Cu2O@CH also exhibited efficient I– and IO3– removal from simulated high-level liquid waste. The rapid capture and effective immobilization of radioiodine (I–, IO3–) were realized by the electrostatic interaction of −N+(CH3)3 groups in Cu2O@CH with I– and IO3–, as well as the chemical reactions between Cu2O and I–. The bulk cationic hydrogel composite explored the multifunctional role toward fast, high adsorption capability and easy handling, highlighting its superiority compared to the powder adsorbent, which renders it a potential adsorbent for the removal of radioactive iodine (I–, IO3–) in nuclear wastewater treatment.
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CHEMOSPHERE
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2023
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IF 8.8
Wenya Dong,Jiaqi Yan,Yaoyu Yang,Qingsheng Wu,Xiaojun Hu
Laccase is an efficient green biocatalyst, widely used for the degradation of various organic pollutants. However, free laccase is unstable and difficult to recover, which limits its practical application. In this study, a multilayer core-shell magnetic mesoporous silica (Fe 3 O 4 @d-SiO 2 @p-SiO 2 ) microsphere with high specific surface area (275 m 2 g −1 ) was fabricated for immobilization of laccase. The unique structure of Fe 3 O 4 @d-SiO 2 @p-SiO 2 enabled the successful immobilization of laccase. Under the optimal immobilization conditions of laccase concentration of 1.5 mg mL −1 , immobilization time of 6 h, immobilization pH of 6, the loading capacity of laccase was up to 567 mg g −1 . Compared with free laccase, immobilized laccase exhibited remarkable pH stability, thermal stability and storage stability. Moreover, the immobilized laccase was easy to achieve magnetic recovery and possessed excellent reusability, with its activity remaining 58.2% after 10 consecutive reuses. More importantly, immobilized laccase had good degradation performance for benzo[a]pyrene (BaP), which can achieve rapid and efficient degradation of low concentration BaP over a wide range of pH and temperature. The removal efficiency of BaP was up to 99.0% within 1 h, and still exceeded 35.0% after 5 cycles. The removal of BaP by immobilized laccase was achieved through both adsorption and degradation. The degradation products and possible degradation pathways were determined by GC-MS analysis. This study indicated that Fe 3 O 4 @d-SiO 2 @p-SiO 2 could effectively enhance the stability and biocatalytic activity of laccase, which is expected to provide a new clean biotechnology for the remediation of BaP contaminated sites.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2023
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IF 7.2
Zihan Tian,Yantao Song,Jing Zhang,Shuhao Qin,Yuanyuan Yang,Jianxin Li,Zhenyu Cui
Organic pollutants, such as dyes, contaminated water bodies seriously. Suitable materials and efficient technologies for degrading pollutants needed to be explored to protect the environment and human health. In this investigation, PVDF(PAA-g-β-CD)@TiO 2 hybrid film was prepared by a combination of “in situ hydrothermal-vapor induction” under mild conditions. The distribution, particle size, and interaction with the film surface for TiO 2 were investigated by XRD , XPS , SEM, and EDS . The TiO 2 nanoparticles were firmly immobilized and uniformly distributed on the hybrid film surface. Poly (acrylic acid) (PAA) and β-cyclodextrin (β-CD) on the film matrix were connected by the chemical bond, which provided stable nucleation and growth sites for TiO 2 while inhibiting photogenerated electron-hole recombination and significantly enhancing the adsorption ability. Furthermore, the multiple interaction forces (hydrogen bonding, coordination, and hydrophilic-hydrophilic interactions) between the film matrix and the TiO 2 were evaluated by simulated geometries and theoretical calculations. The multiple interactions reduced the band gap (Eg) while endowing the hybrid photocatalytic film with excellent degradation efficacy ( η ), stability, and recycling properties. Finally, the η of methylene blue (MB) reached 97.34 % within 120 min and remained above 90 % even after 8 reuses. It was believed that PVDF(PAA-g-β-CD)@TiO 2 film provided an approach for the mild preparation of polymer/TiO 2 hybrid film with high catalytic activity and optimization of photocatalytic properties.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Dongmin Zhao,Zhuoyan Li,Kaini Zhu,Ai Lu,Ying Wang,Jingjing Jiang,Cong Tang,Xing-Can Shen,Changping Ruan
Owing to the strong Hg−Se interaction, Se-containing materials are promising for the uptake and immobilization of Hg(II) ions; compared with metal selenides or selenized compounds, elemental Se contains the highest ratio of Se. However, it remains a challenge to fully expose all the potential Se binding sites and achieve high utilization efficiency of elemental Se. Through rational design on the structure, dispersity, and size of materials, Se/CNF aerogels composed of abundant well-dispersed and amorphous nano-Se have been prepared and applied for the high-efficient uptake and immobilization of Hg(II) ions. The well-dispersion of nano-Se increases the exposure of Se sites, the amorphous structure benefits the easy cleavage of Se−Se bonds, the 3D porous networks of aerogels permit fast ions transport and easy operation. Benefiting from the combination effect of strong Hg−Se interaction and sufficient exposure of Se-enriched sites, the Se/CNF aerogels demonstrate strong binding ability ( K d = 3.8 ×10 5 mL·g -1 ), high capacity (943.4 mg·g -1 ), and preeminent selectivity ( α M Hg > 100) towards highly toxic Hg(II) ions. Notably, the utilization efficiency of Se in Se/CNF aerogels is as high as 99.5%. Moreover, the strong Hg−Se interaction and extraordinary stability of HgSe could minimize the environmental impact of the spent Se/CNF adsorbents after its disposal.
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SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Weidong Qin,Junhua Wang,Zhanqiu Tang,Hongyuan Tian,Zhenglong Wu
Quick and efficient immobilization of DNAs on gold nanoclusters (AuNCs) has a substantial impact on nanoscale applications relative to molecular recognition and patterned assembly. We found that in the presence of tris(2-carboxyethyl)phosphine (TCEP), chemisorption of thiolated DNA on the AuNC surface could be accomplished within 1.5 h, which was dramatically shorter than the incubation time needed in the conventional thiolate exchange reactions. Based on this observation, a core-shell-satellite nanocomposite consisting of magnetic nanoparticle , silica coating, and AuNCs (Fe 3 O 4 NPs@SiO 2 @AuNCs) was fabricated, on which a fluorescent hairpin probe was anchored with the assistance of TCEP. Integrated with duplex-specific nuclease (DSN)-assisted target recycling strategy, the sensing platform could simultaneously detect microRNA-21 and microRNA-141 with detection limits of 0.020 and 0.017 pM, respectively. The feasibility of the developed immobilization approach might significantly advance the use of DNAs and AuNCs in analytical sciences and clinical research.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Siqin He,Bin Xu,Ying Liu,Xiaoshuang Yin,Wenzhong Yang,Yun Chen
Polyaminoamide (PAMAM) dendrimers with different generations from 1 G to 5 G were grafted on magnetic core-shell Fe 3 O 4 -SiO 2 composite particles , respectively. Using Fe 3 O 4 as a magnetic center and SiO 2 as the connecting layer, the PAMAM dendrimers with amino functional groups were modified on SiO 2 successfully, while laccase was immobilized to the surface of Fe 3 O 4 -SiO 2 -PAMAM x by covalent cross-linking using amide bond. Under the optimal conditions, the loading capacity of laccase reached 65.5 mg/g, and about 62.8 % of its initial activity of free laccase was reserved. Immobilized laccase degradated 2,4-dichlorophenol (2,4-DCP) with a degradation ratio of 91.5 % after 24 h. The degradation ratio of 82.9 % was maintained after 5 cycles of the operation, and when enhanced to the 10 cycles degradation, the degradation rate was still remained close to 80 %. Compared with free laccase, the comprehensive stability of immobilized laccase was improved significantly, and it displayed the excellent catalytic degradation ability for 2,4-DCP.
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Journal of Environmental Sciences
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2023
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IF 6.9
Mei Du,Jingzhang Liu,Qiong Wang,Fengbang Wang,Lei Bi,Chunyan Ma,Maoyong Song,Guibin Jiang
Endocrine disruptors such as bisphenol A (BPA) adversely affect the environment and human health. Laccases are used for the efficient biodegradation of various persistent organic pollutants in an environmentally safe manner. However, the direct application of free laccases is generally hindered by short enzyme lifetimes, non-reusability, and the high cost of a single use. In this study, laccases were immobilized on a novel magnetic three-dimensional poly(ethylene glycol) diacrylate (PEGDA)–chitosan (CS) inverse opal hydrogel (LAC@MPEGDA@CS@IOH). The immobilized laccase showed significant improvement in the BPA degradation performance and superior storage stability compared with the free laccase. 91.1% of 100 mg/L BPA was removed by the LAC@MPEGDA@CS@IOH in 3 hr, whereas only 50.6% of BPA was removed by the same amount of the free laccase. Compared with the laccase, the outstanding BPA degradation efficiency of the LAC@MPEGDA@CS@IOH was maintained over a wider range of pH values and temperatures. Moreover, its relative activity of was maintained at 70.4% after 10 cycles, and the system performed well in actual water matrices. This efficient method for preparing immobilized laccases is simple and green, and it can be used to further develop ecofriendly biocatalysts to remove organic pollutants from wastewater.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Yuan-song Zhang,Xuechao Xu,Juanli Yang,Mengyuan Tan,Wenyuan Zhou,Lu Gao,Zhen-quan Yang
Cronobacter sakazakii in powdered infant formula (PIF) can cause infections in newborns that are life-threatening. Establishing a High-performance evaluation method for C. sakazakii in the PIF is urgently needed. In this study, a chromogenic system based on phage-directed immobilized nanozyme for colorimetric measurement of C. sakazakii in PIF was constructed . Initially, our acquired phage EspYZU15 was electrostatically immobilized on the surface of a palladium-based nanozyme to produce EspYZU15 directionally immobilized Pd (EspYZU15@Pd). EspYZU15@Pd possesses outstanding peroxidase-like properties, catalyzing the chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . The ability of EspYZU15@Pd to recognize and trap host bacteria allows C. sakazakii to specifically inhibit the TMB chromogenic process catalyzed by EspYZU15@Pd. Based on this detection strategy, a visual quantification method for C. sakazakii ranging from 3.2 × 10 2 to 3.2 × 10 7 CFU mL −1 was developed, yielding a low limit of detection (LOD) of 3.6 × 10 1 CFU mL −1 . This method was able to successfully detect C. sakazakii in PIF, with outstanding recoveries ranging from 95.8% to 104.9%. These characteristics indicate that the EspYZU15@Pd-based chromogenic system has considerable promise for the colorimetric determination of C. sakazakii in PIF.
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