Biosensors-Basel
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2022
·Vol. 12
·期 2
·66
·
IF 5.743
Bo Wu,Zehua Li,Zepeng Kang,Chunling Ma,Haiyan Song,Fuping Lu,Zhiguang Zhu
D-2-hydroxyglutaric acid (D2HG) is overproduced as a result of the D-2-hydroxyglutaric aciduria and relevant cancers, caused by gene mutation. Accurate analysis of D2HG could help rapid diagnosis of these diseases and allow for timely treatment. In this work, a D-2-hydroxyglutarate dehydrogenase fromRalstonia solanacearum(RsD2HGDH) is cloned and recombinantly expressed. This enzyme features the direct electron transfer to chemical electron mediators (such as methylene blue (MB)) in the absence of additional coenzymes. Therefore, NAD+, a natural electron acceptor for the commercial D2HGDH and usually known for being unstable and difficult for immobilization can be avoided in the preparation of biosensors. TheRsD2HGDH and MB are co-immobilized on a two-dimensional material, Ti3C2MXene, followed by drop-coating on the gold screen-printed electrode (AuSPE) to construct a compact and portable biosensor. The D2HG in samples can be catalyzed byRsD2HGDH, where the current change is measured by chronoamperometry at −0.23 V. The biosensor shows a D2HG detection range of 0.5 to 120 µM (R2= 0.9974) with a sensitivity of 22.26 μA mM−1cm−2and a detection limit of 0.1 µM (S/N = 3). The biosensor retains 72.52% performance of its incipient state after 30 days of storage. The samples of D2HG-containing fetal bovine serum and artificial urine were analyzed with the recovery of 99.56% to 106.83% and 97.30% to 102.47% further indicating the great application potential of our portable D2HG biosensor.Keywords:D-2-hydroxyglutaric acid;MXene;biosensor;screen-printed electrode;D-2-hydroxyglutarate dehydrogenase
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高影响因子
BRAIN RESEARCH
·
2021
·
IF 3.252
Shuai-Nan Zheng,Long Pan,Ai-Mei Liao,Yin-Chen Hou,Guang-Hai Yu,Xiao-Xiao Li,Yong-Jian Yuan,Yu-Qi Dong,Zi-Shan Zhang,Cui-Zhu Tian,Zeng-Liang Liu,Wen-Jin Lin,Ming Hui,Jian Cao,Ji-Hong Huang
Wheat embryo globulin nutrient (WEGN), with wheat embryo globulin (WEG) as the main functional component, is a nutritional combination that specifically targets memory impairment. In this study, we explored the protective role of WEGN on Alzheimer's disease (AD)-triggered cognitive impairment, neuronal injury, oxidative stress, and acetylcholine system disorder. Specifically, we established an AD model via administration of d -galactose ( d -gal) and Aluminum chloride (AlCl 3 ) for 70 days, then on the 36th day, administered animals in the donepezil and WEGN (300, 600, and 900 mg/kg) groups with drugs by gavage for 35 days. Learning and memory ability of the treated rats was tested using the Morris water maze (MWM) and novel object recognition (NOR) test, while pathological changes and neuronal death in their hippocampus CA1 were detected via HE staining and Nissl staining. Moreover, we determined antioxidant enzymes by measuring levels of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) in serum, cortex, and hippocampus, whereas changes in the acetylcholine system were determined by evaluating choline acetyltransferase (ChAT), and acetylcholinesterase (AChE) activities, as well as choline acetylcholine (Ach) content. Results revealed that rats in the WEGN group exhibited significantly lower escape latency, as well as a significantly higher number of targeted crossings and longer residence times in the target quadrant, relative to those in the model group. Notably, rats in the WEGN group spent more time exploring new objects and exhibited lower damage to their hippocampus neuron, had improved learning and memory activity, as well as reversed histological alterations, relative to those in the model group. Meanwhile, biochemical examinations revealed that rats in the WEGN group had significantly lower MDA levels and AChE activities, but significantly higher GSH, SOD, and ChAT activities, as well as Ach content, relative to those in the model group. Overall, these findings indicate that WEGN exerts protective effects on cognitive impairment, neuronal damage, oxidative stress, and choline function in AD rats treated by d -gal/AlCl 3 .
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高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yuling Sun,Jie Liu,Zhibo Li,Jianjun Wang,Yanbin Huang
Water-soluble, biodegradable, nonionic, and biocompatible polymers with multiple functional groups are highly desired for biomedical applications. Here, we report that water-soluble nonionic poly(d/l-serine) is chirality-controllable, biodegradation-controllable, and non-cytotoxic. Hence, it can be a highly sought-after alternative to the widely used poly(ethylene glycol), with an additional advantage of having multiple hydroxyl groups for further functionalization. As one example of its biomedical applications, poly(d/l-serine) demonstrated an obvious cryoprotective effect on the red blood cells. The usage of poly(d/l-serine) in the cryopreservation field would be of great promise to resolve the difficulties in separating cryoprotectants due to toxicity.
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高影响因子
Advanced Optical Materials
·
2021
·Vol. 9
·期 17
·2100622
·
IF 9.926
Yanhui Sun,Hui Li,Xiangyun Gao,Zhiyang Yu,Zhipeng Huang,Chi Zhang
The exploitation of high-performance nonlinear optical (NLO) materials is necessitated by the broad application of ultra-intensive and ultra-fast lasers. The superb nonlinear absorption and electronic-structure-related NLO response of M 3 HHTP 2 are introduced here, where M is Co(II), Ni(II), or Cu(II) and HHTP is 2,3,6,7,10,11-hexahydroxytriphenylene. With the laser excitation at 532 nm, the Cu 3 HHTP 2 exhibits a two-photon absorption (2PA) cross-section of 1.38 ± 0.11 × 10 7 GM, a value much larger than those of most reported NLO materials so far. The NLO response of M 3 HHTP 2 correlates strongly with the number of metal d electrons. With metal d electrons number increasing from 7 to 10, the optical nonlinearity of M 3 HHTP 2 under the laser excitation at 532 nm varies from three-photon absorption (3PA) in Co 3 HHTP 2 , a combination of 3PA and 2PA in Ni 3 HHTP 2 , to 2PA in Cu 3 HHTP 2 . Abundant metal d electrons can mitigate the ground-state depletion, and therefore the 2PA is enhanced. Accordingly, the superb nonlinear absorption of Cu 3 HHTP 2 can be associated with abundant d electrons in Cu.
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高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Huaxia Li,Wencui Chai,Yijun Cao,Yankun Wu,Shichong Yang
Mixed collectors were widely used in mineral flotation. In this work, D-phenylalanine (D-phe) was first introduced to improve the collectability and selectivity of sodium oleate (NaOL) for diaspore from kaolinite. The flotation performance of diaspore and kaolinite were studied by micro-flotation tests, and the synergistic mechanisms of D-phe with NaOL were investigated through FTIR spectra, zeta potential, solution chemistry and X-ray photoelectron spectroscopy (XPS). Single and mixed flotation results show that D-phe exhibited superior synergism power with the NaOL collector. Under pulp pH 9.0, the flotation recovery of diaspore was increased from 77.13% to 91.07%, while that of kaolinite was decreased from 16.27% to 13.75%. The zeta potential and XPS analysis indicate that the synergistic mechanisms of D-phe with NaOL were mainly the co-adsorption via the carboxylate group of D-phe and NaOL bonding with the Al sites of diaspore surface. D-phe was chelated onto the diaspore surface through the –COO – and –NH 3 + /NH 2 groups, and the pre-adsorption of D-phe could increase the Al active sites of diaspore surface for the direct adsorption of NaOL. NaOL could also indirectly adsorb on the diaspore surface through H-bonding with –NH 2 /NH 3 + groups of D-phe.
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GREEN CHEMISTRY
·
2020
·Vol. 23
·期 3
·1382-1388
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IF 9.48
Liang Chen,Yiming Huang,Ren Zou,Jiliang Ma,Yunyi Yang,Tingzhen Li,Mingsai Li,Qi Hao,Haibo Xie,Xinwen Peng
Synthesis of D-xylonic acid, a high value chemical feedstock, from biomass chemicals through a photocatalytic process is of great significance in respect of economical and sustainable concerns, and therefore has aroused considerable attention. Herein, a TiO2/Ti3C2(TT) photocatalyst was designed for the oxidation of xylose to D-xylonic acid under ambient conditions. The photocatalyst is fabricated by in situ growth of TiO2 nano-particles on Ti3C2 nanosheets and exhibits 64.2% of D-xylonic acid yield with excellent stability. The mechanistic study indicates that the electron transfer from TiO2 to Ti3C2 significantly increases the life time of light-induced charge carriers, and hence improved photocatalytic performance.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Teng Li,Ping Jin,Yekai Zhang,Kexin Huang,Jingen Long,Jun Jiang,Shengqin Liu,Ayusman Sen,Weiran Yang
As an important biomass-derived chemical, d-sorbitol can be converted to a variety of useful chemicals and fuels. Because of the strong nucleophilicity of I– and reducibility of hydrogen iodide (HI), hydroiodic acid has been used for polyol hydrogenolysis with high selectivity. Here, d-sorbitol was converted by HI, rhodium, and hydrogen to give a high yield of iodohexanes (94.2%) at 373 K in a water/cyclohexane biphasic system. Rhodium and hydrogen were used to regenerate HI in situ. The reaction mechanism was studied in detail by using model molecules. The substitution–elimination–addition (SEA) mechanism was proven to be the most possible pathway. The high concentration of both proton and iodide was found to be necessary for the efficient conversion of d-sorbitol and the selective formation of iodohexanes. Besides, water could inhibit the conversion of d-sorbitol dramatically. These findings were explained by kinetic study and demonstrated in the reaction kinetic equation. This catalytic system, including HI and RhCl3, was proven to be reusable. In addition, iodohexanes can be quantitatively converted to hexenes over ZrO2 at 473 K.
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高影响因子
PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Xuelian Zhou,Yi Shen,Yong Xu,Venkatesh Balan
2-Keto- d -gluconic acid (2-KGA) is a high-value-added product from lignocellulosic straw-based biorefining process obtained via cell catalysis using Gluconobacter oxydans ( G. oxydans ); nonetheless, the economical production has some limitations because of the formation of undesirable mixture products of keto-gluconic acid from glucose. In this study, it was demonstrated that pulse addition of hydrogen peroxide (H 2 O 2 ) to synthetic media was beneficial to improve the conversion rate of glucose to 2-KGA by 1.47-fold in G. oxydans . Moreover, some lignocellulosic degradation compounds served as substitute and played the role of H 2 O 2 on corn stover hydrolysate for the production of 2-KGA. By conducting qRT-PCR analysis, eight dehydrogenase genes were identified in G. oxydans , which were responsible for regulating 2-KGA production. Moreover, most of the identified genes were found to produce membrane-bound glucose dehydrogenase and gluconate 2-dehydrogenase, and were up-regulated by adding H 2 O 2 or degradation compounds. These up-regulated genes could be responsible for directing cell catalysis of glucose to 2-KGA in G. oxydans .
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高影响因子
CATALYSIS LETTERS
·
2020
·Vol. 151
·期 2
·338-343
·
IF 2.482
Shi Hexian,Zhang Linfeng,Wu Yudong,Yu Rui,Peng Yang,Wang Yantao,Li Teng,Yang Weiran
Abstract Glucose and its derivatives are valuable and sustainable carbon sources that can be converted into varieties of value-added chemicals. In this study, d -glucaric acid was selectively converted to 3-iodoadipic acid (3-IAA) by hydrodeoxygenation in HI-Rh/C-H 2 system. During this process, HI was a reducing reagent, which can be regenerated in situ by metal catalyzed hydrogenation. The reaction pathway was proposed based on the intermediates identification. Under the optimal conditions, 67.2% yield of 3-IAA was obtained at 130 ℃ for 4 h. As a platform molecule, 3-IAA can be further converted to varieties of building block efficiently, such as adipic acid (AA) and 3-hydroxyadipic acid (3-HAA). Graphic Abstract
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Yan Xia,Xiang-Mei Xiang,Kang-Yu Dong,Yan-Yan Gong,Zhan-Jun Li
Microplastics (MPs) have aroused rising social concerns. Although amounts of surfactants exist in wastewater and are expected to alter the surface properties of MPs significantly as they are designed to be adsorbed by hydrophobic particles. However, rare works have been done on the influence of surfactants on the coagulation removal process of MPs which was thought to be an effective way to remove MPs together with other natural particles, such as clay. We used 3-D fluorescence imaging to track the coagulation removal process of polystyrene MPs. Our results indicate that nonionic surfactant, tween 20 in ppm scale, could inhibit the coagulation removal of polystyrene MPs significantly. Residue MPs in the effluent is proportional with the surfactant concentration and increases up to tens of times, which will lead to a dramatic increase in their potential environmental risks. Apparent size effect exists in the coagulation in which smaller MPs can escape from the coagulation removal more easily. Mechanism study suggests that the steric resistance of the hydrophilic flexible polyethylene glycol (PEG) layer formed by tween 20 adsorbed on MP surface inhibits clay deposition and thus hinders subsequent agglomeration and precipitation. A surfactant stealth effect, which is used in the design of nanomedicine to avoid the human immune recognition and clearance of nano-drugs from blood circulation , also exists in the coagulation removal process of MPs. Our finding not only proves the strong influence of surfactants on MPs but also will stimulate related studies on other latent surfactant effects of MPs.
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高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 80
·期 4
·1868-1876
·
IF 4.1
Xiaoqin Cheng,Qiang Wen,Yunna Li,Shanshan Wang,Gang Fan,Zhaocheng Ma,Yuancheng Guo,Xiaoxue Li,Hongyu Zhang
BACKGROUND Bactrocera minax is a devastating pest of citrus fruits. However, there have been no effective control measures before. Few reports on the sex pheromones of B. minax are available. RESULTS In this study, nine of the volatile compounds in adult females were identified using headspace solid-phase microextraction (HS-SPME) in combination with gas chromatography–mass spectrometry (GC–MS). Among them, d-limonene, caprolactam, 2-Nitro-1H-imidazole, and creatinine could evoke antennal responses in males. Field bioassays showed that only d-limonene could lure male flies, with a relative lure rate of 78.18% in all tested samples, which was significantly higher than that of paraffin oil control, while all volatile compounds did not have any lure effective to female flies. Moreover, d-limonene was diluted with paraffin oil into differential concentrations, the lure effect on males was better at 100, 500, and 800 μL d-limonene mL −1 than pure d-limonene (1000 μL mL −1 ). The relative male lure rate of d-limonene at 100 μL mL −1 was 85.88%, which was significantly higher than that of food−baits (14.12%) on day 3. However, d-limonene was unattractive to female and male Bactrocera dorsalis and Zeugodacus tau . Further kinetic analysis showed that female adults released d-limonene around 15-day post eclosion. Electroantennography 1 results showed that 500 μL mL −1 d-limonene evoked the strongest responses to antennae of 10- to 25-day-old male flies. CONCLUSION Our results indicated that d-limonene could be a sex pheromone from female flies of B. minax , and it could be used as a male-specific sex attractant for B. minax. © 2023 Society of Chemical Industry.
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高影响因子
Journal of Materials Chemistry A
·
2023
·Vol. 12
·期 7
·3967-3976
·
IF 11.9
Surong Zheng,Shiwei Yu,Zaka Ullah,Lei Liu,Ledi Chen,Houliang Sun,Mingliang Chen,Liwei Liu,Qi Li
All-solid-state lithium-ion batteries have great scope in the development of advanced secondary batteries owing to their high energy density and nonflammability. However, high contact resistance at the solid/solid (electrode/electrolyte) interface and the possible existence of side reactions limit their large-scale applications. In this study, a π-d conjugated coordination structure was established between a cathode and electrolyte: 2,5-dihydroxy-1,4-benzoquinone (DHBQ) was the cathode and polyvinylidene fluoride-based Li0.33La0.557TiO3 (PVDF-LLTO) polymer was the solid-state electrolyte. First-principle calculations and performed experiments showed that CO and O–H groups in DHBQ can be coordinated with La3+ in LLTO, and this π-d conjugate coordination structure strengthens the contact interface between the electrode material and solid electrolyte, which further increases the cycling life and durability of all-solid-state batteries.
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危险品化学品经营许可证（带存储）