Foods
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2021
·Vol. 10
·期 9
·2123
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IF 4.35
Yong Sun,Fanghua Guo,Xin Peng,Kejun Cheng,Lu Xiao,Hua Zhang,Hongyan Li,Li Jiang,Zeyuan Deng
Tetrastigma hemsleyanumDiels et Gilg is a herbaceous perennial species distributed mainly in southern China. TheTetrastigma hemsleyanumroot (THR) has been prevalently consumed as a functional tea or dietary supplement. In vitro digestion models, including colonic fermentation, were built to evaluate the release and stability of THR phenolics with the method of HPLC-QqQ-MS/MS and UPLC-Qtof-MS/MS. From the oral cavity, the contents of total phenolic and flavonoid began to degrade. Quercetin-3-rutinoside, quercetin-3-glucoside, kaempferol-3-rutinoside, and kaempferol-3-glucoside were metabolized as major components and they were absorbed in the form of glycosides for hepatic metabolism. On the other hand, the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, and glutathione (GSH) content were significantly increased, while malondialdehyde (MDA) content was decreased in plasma and tissues of rats treated with THR extract in the oxidative stress model. These results indicated that the THR extract is a good antioxidant substance and has good bioavailability, which can effectively prevent some chronic diseases caused by oxidative stress. It also provides a basis for the effectiveness of THR as a traditional functional food.Keywords:Radix Tetrastigma;colonic fermentation;phenolics;antioxidant capacity;bioavailability
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INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
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2018
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IF 1.372
Yuanyuan Ding,Jianhong Yang,Jun Cai
Guanidinylated carboxymethyl chitosan (GCMCS) was prepared via the guanidinylation of carboxymethyl chitosan (CMCS). A device employing the diffusive gradients for thin films (DGT) technique was made using a GCMCS aqueous solution as the binding agent and a cellulose acetate dialysis membrane (CADM) as the diffusion phase to measure labile Cu2+, Pb2+ and Cd2+ in water. The percentage uptake (U%) values of labile Cu2+, Pb2+ and Cd2+ in a synthetic water sample were almost consistent with the theoretical values at 101.6 ± 2.8%, 104.6 ± 6.1% and 95.9 ± 4.4%, respectively. The optimum pH ranges for the measurement of labile Cu2+, Pb2+ and Cd2+ were 3.0–7.0, 3.0–7.0 and 4.0–8.0, respectively. The ionic strength mainly affected the diffusion of metal ions in the CADM. The diffusion rates decreased with increasing concentrations of NaNO3 solutions. The application of GCMCS-DGT in natural water and industrial wastewater showed that dissolved organic carbon (DOC) only affects metal species, and the accurate determination of labile Cu2+, Pb2+ and Cd2+ can be achieved when the diffusion coefficients of these metal ions in the diffusion phase have been determined. GCMCS is suitable for DGT application as a chelating agent for metal ions.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2017
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IF 4.233
Fangyuan Gai,Li Yin,Mengmeng Fan,Ling Li,Johnny Grahn,Yuhui Ao,Xudong Yang,Xuming Wu,Yunling Liu,Qisheng Huo
This work demonstrated the synthesis of (4E)-4-(4-(diphenylamino)benzylideneamino)-1,2-dihydro-1,5- dimethyl-2-phenylpyrazol-3-one (DBDDP) for Fe (III) detection in aqueous media and in the core of silica cross-linked micellar nanoparticles in living cells. The free DBDDP performed fluorescence enhancement due to Fe (III)-promoted hydrolysis in a mixed aqueous solution, while the DBDDP-doped silica cross-linked micellar nanoparticles (DBDDP-SCMNPs) performed an electron-transfer based fluorescence quenching of Fe (III) in living cells. The quenching fluorescence of DBDDP-SCMNPs and the concentration of Fe (III) exhibited a linear correlation, which was in accordance with the Stern-Volmer equation. Moreover, DBDDP-SCMNPs showed a low limit of detection (LOD) of 0.1 ppm and an excellent selectivity against other metal ions. Due to the good solubility and biocompatibility, DBDDP-SCMNPs could be applied as fluorescence quenching nanosensors in living cells.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Kehan Zhu,Zhen Yao,Tianyi Gu,Xianhao Jiang,Jianqin Zhou,Duxin Li
Swollen culms of Zizania latifolia possess a peculiar flavor, nutritional value and health-promoting effects. This study investigated the wound healing ability of polysaccharides isolated from Zizania latifolia (ZLP). Through in vitro assays, ZLP demonstrated negligible cytotoxicity and promoted the migration and phagocytosis of Staphylococcus aureus by macrophages where ZLP-2 had a significantly greater effect. Moreover, ZLP-2 has a higher purity and molecular weight, an integral and large lamellar morphology, and a larger particle size in solution, suggesting its ability to form films. Thus, ZLP-2 was fabricated as a gel formulation for topical application. The in vivo results showed that the ZLP-2 gel had significantly greater effects than did the autonomous healing process according to quantitative analysis of the wound closure area. The ZLP-2 gel decreased the levels of TNF-α and IL-6 in wounded tissue. Furthermore, the quantification of the molecular indexes via qRT-PCR revealed that the levels of tnf-α, tgf-β, mcp-1, ifn-γ, inos, and vegf in the treated group increased during the inflammatory and proliferation stages but decreased after wound closure. These results suggest that ZLP-2 has great potential in pharmaceutical applications for wound care.
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Analytical Methods
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2025
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IF 2.6
Zhaoyang Gu,Yongming Huang,Beichen Lin,Kunde Lin
Current methods for determining dissolved reactive phosphorus (DRP) in sediment porewater mainly rely on ex-situ analysis, which can be labor-intensive and introduces errors due to changes in the concentration and morphology during sampling and transportation. Therefore, there is a clear need for in-situ measurement methods for DRP in sediment porewater. In this study, a novel in-situ analytical method was developed by combining diffusive gradients in thin-films (DGT) and digital imaging techniques, based on the classic phosphomolybdenum blue (PMB) method. During deployment, DRP in sediment porewater diffuses into the DGT device, while reducing reagent (ascorbic acid) from the diffusive gel simultaneously diffuses into the binding gel and reacts with ammonium molybdate, resulting in the formation of PMB compounds. The color of the PMB compounds was recorded in-situ using a digital imaging technique, and the resulting images were analyzed to calculate the concentration of DRP in surface sediment porewater. By using the new method, analytical data of DRP in surface sediment porewater could be quickly obtained in the field near real-time, without water sampling and any further sample treatment, transportation and instrumental analysis in laboratories. The key parameters including the loading procedure for colorimetric reagents in the gels, the concentrations of ammonium molybdate and ascorbic acid, the amount of C18, and the thickness of the C18 binding gel, were systematically optimized. The optimized method demonstrated a wide linear range (4 ‒ 1000 μmol L-1, R2=0.993), with satisfactory accuracy (-6.23% ‒ 8.68%, n = 3) and good precision (6.60% ‒ 13.46%, n = 3) at an exposure time of 60 min. The method also performed well across different salinities (0 ‒ 35) and in the presence of interfering ions (SiO32‒, AsO33‒, F‒, and S2‒). The method was successfully applied to both freshwater and seawater sediments, with results comparable to those obtained using traditional analytical methods. Overall, the proposed method provides a new strategy for in-situ determination of DRP in surface sediment porewater.
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ACS Sustainable Chemistry & Engineering
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2025
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IF 7.3
Fang Li,Pengfei Du,Haili Lin,Huiqin Yu,Yang Wang,Bin Fang,Jing Cao
Replacing the anodic oxygen evolution reaction with electro-oxidation of glycerol for hydrogen energy not only reduces energy consumption but also obtains high-value chemicals. However, this system still faces bottlenecks of limited Faradaic efficiency, low selectivity, and an unclear reaction pathway. Herein, the Mott–Schottky Ni(OH)2–In heterostructure was prepared for selective glycerol electro-oxidation (GOR). A series of experiments and theoretical calculations validated the charge shuttling through a heterointerface and the formation of the built-in electric field (BIEF) structure. As expected, the Ni(OH)2–In delivered a low potential of 1.22 V vs RHE at 10 mA cm–2. The Faradaic efficiency and selectivity for formate are up to 93% and 95%, respectively, together with long-term stability for the 20 cycles. The excellent performance can be attributed to the changed reaction pathway of producing the formate, reduced reaction energy barrier of the rate-determining step (RDS), and a more rapid GOR kinetics which arise from the robust BIEF. This work indicates that via constructing BIEF in the Mott–Schottky heterostructure, the electrocatalytic glycerol oxidation can be promoted.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Buqing Wang,Xiaoman Zhou,Yasen Wang,Yahui Gao,Hideki Nakanishi,Morihisa Fujita,Zijie Li
The acidic lipase from Rasamsonia emersonii named LIPR has great potential for biodiesel synthesis due to its strong methanol tolerance. Nonetheless, the limited thermostability of LIPR and low expression level in Escherichia coli remain major obstacles to its use in biodiesel synthesis. To enhance the thermostability, the mutant LIPR harboring mutations A126C-P238C for the formation of a new disulfide bond and amino acid substitution D214L was obtained through rational design. To our delight, the thermostability of LIPR mutant was greatly improved. Moreover, a comprehensive optimization strategy, such as employing the Mss signal peptide, co-expressing the molecular chaperone protein disulfide isomerase (PDI), knocking out the vacuolar sorting receptor gene VPS10-01 , and overexpressing the dihydroxyacetone synthase gene DAS2 , was adopted to obtain the combination-optimized mutant Pichia pastoris strain GS54. Furthermore, the biodiesel synthetic capability with the mutant GS54-LIPR was verified and the production yield was 52.2 % after 24 h in a shake flask. Subsequently, a continuous flow system was adopted to increase the biodiesel yield to 73.6 % within 3 h, demonstrating its efficacy in enhancing enzyme biocatalysis. The engineered GS54-LIPR mutant lipase is an efficient and reusable biocatalyst for the sustained production of biodiesel in a continuous flow reaction.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2019
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IF 3.17
Yaqian Zhou,Junjie Zhou,Ping Li,Qiushi Xie,Binbin Sun,Ying Li,Yang Chen,Kaijing Zhao,Tingting Yang,Liang Zhu,Jiong Xu,Xiaodong Liu,Li Liu
Liver failure altered P-glycoprotein (P-gp) function and expression at blood-brain barrier (BBB), partly owing to hyperammonemia . We aimed to examine the effects of partial portal vein ligation (PVL) plus chronic hyperammonemia (CHA) on P-gp function and expression at rat BBB. Experimental rats included sham-operation (SH), PVL, CHA and PVL+CHA. The PVL+CHA rats were developed by ammonia-containing diet for 2 weeks after operation. The brain-to-plasma concentration ratios (K p ) and apparent unidirectional influx constants (K in ) of rhodamine123 and sodium fluorescein were measured to assess function of P-gp and BBB integrity, respectively. Human cerebral microvascular endothelial cells (HCMEC/D3) were used to assess effects of ammonia on P-gp expression and function. It was found that PVL+CHA significantly decreased K p and K in of rhodamine123 without affecting brain distribution of fluorescein. The P-gp expressions in membrane protein in cortex and hippocampus were significantly increased in CHA and PVL +CHA rats, especially in PVL + CHA rats, while remarkably increased phosphorylated ERK1/2 was only found in PVL +CHA rats. Expressions of tight junction proteins claudin-5 and occluding in rat brain remained unchanged. In vitro data showed that NH 4 Cl increased reactive oxygen species , membrane expression and function of P-gp as well as phosphorylated ERK1/2 levels in HCMEC/D3. The NH 4 Cl-induced alterations were reversed by reactive oxygen species scavenger N-acetylcysteine and ERK1/2 inhibitor U0126 . In conclusion, PVL+CHA increased function and membrane translocation of P-gp at rat BBB partly via ammonia. Reactive oxygen species/ERK1/2 pathway activation may be one of the reasons that ammonia upregulated P-gp expression and function at BBB.
研究领域:
药理学
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神经科学
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病理生理学
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分子生物学
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Nutrients
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2024
·Vol. 16
·期 4
·514
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IF 4.8
Xiao Wang,Yongjie Liang,Bohan Zhang,Lixin He,Wenxuan Li,Wenwen Zhang,Chengzong Li,Lihong Luo,Talha Umar,Huili Feng,Changwei Qiu
2’-Hydroxychalcone is a hydroxyl derivative of chalcones, which are biosynthetic precursors of flavonoids and rich in the human diet. The anticancer activity of 2’-hydroxychalcone has been reported in several cancers but remains to be investigated in breast cancer. In the current study, 2’-hydroxychalcone showed significant cytotoxicity against breast cancer cell lines MCF-7 and CMT-1211. It could inhibit breast cancer cell proliferation, migration, and invasion in vitro and suppress tumor growth and metastasis in vivo. Mechanistic investigation revealed that the NF-κB pathway was significantly inhibited by 2’-hydroxychalcone treatment accompanied by an excessive intracellular accumulation of reactive oxygen species, induction of endoplasmic reticulum stress, and activation of JNK/MAPK. In addition, 2’-hydroxychalcone elevated the autophagic levels in breast cancer cells equipped with increasing numbers of autophagy vesicles and complete autophagic flux. Finally, autophagy-dependent apoptosis was observed in 2’-hydroxychalcone-induced cell death. In conclusion, 2’-hydroxychalcone enhances the autophagic levels and induces apoptosis in breast cancer cells, which could be contributed to the inhibition of the pro-survival NF-κB signaling, indicating a promising potential for 2’-hydroxychalcone in future anticancer drug development.
研究领域:
肿瘤学
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药理学
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细胞生物学
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分子生物学
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Cancer Cell International
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2021
·Vol. 21
·期 1
·1-15
·
IF 5.722
Lv Minchao,Xu Qingxin,Zhang Bei,Yang Zhiqiang,Xie Jun,Guo Jinku,He Feixiong,Wang Wei
Background Osteosarcoma is the third most common cancer in adolescence and the first common primary malignant tumor of bone. The long-term prognosis of osteosarcoma still remains unsatisfactory in the past decades. Therefore, development of novel therapeutic agents which are effective to osteosarcoma and are safe to normal tissue simultaneously is quite essential and urgent. Methods Firstly, MTT assay, cell colony formation assay, cell migration and invasion assays were conducted to evaluate the inhibitory effects of imperatorin towards human osteosarcoma cells. RNA-sequence assay and bioinformatic analysis were then performed to filtrate and assume the potential imperatorin-induced cell death route and signaling pathway. Moreover, quantitative real-time PCR assay, western blot assay and rescue experiments were conducted to confirm the assumptions of bioinformatic analysis. Finally, a subcutaneous tumor-transplanted nude mouse model was established and applied to evaluate the internal effect of imperatorin on osteosarcoma by HE and immunohistochemistry staining. Results Imperatorin triggered time-dependent and dose-dependent inhibition of tumor growth mainly by inducing autophagy promotion and G0/G1 phase arrest in vitro and in vivo. Besides, imperatorin treatment elevated the expression level of PTEN and p21, down-regulated the phosphorylation of AKT and mTOR. In contrast, the inhibition of PTEN using Bpv (HOpic), a potential and selective inhibitor of PTEN, concurrently rescued imperatorin-induced autophagy promotion, cell cycle arrest and inactivation of PTEN-PI3K-AKT-mTOR/p21 pathway. Conclusions This work firstly revealed that imperatorin induced autophagy and cell cycle arrest through PTEN-PI3K-AKT-mTOR/p21 signaling pathway by targeting and up-regulating PTEN in human osteosarcoma cells. Hence, imperatorin is a desirable candidate for clinical treatments of osteosarcoma.
研究领域:
肿瘤学
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药理学
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细胞生物学
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分子生物学
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EUROPEAN JOURNAL OF HISTOCHEMISTRY
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2022
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IF 1.966
Haixia Li,Ning Su,Yaqiao Zhu,Wei Wang,Meihong Cai,Xiaohuan Luo,Wei Xia,Song Quan
Recurrent implantation failure (RIF) is defined as failure to achieve clinical pregnancy after at least 3 transfers of good-quality embryos by natural or artificial means. RIF is often a complex problem with a wide variety of etiologies and mechanisms as well as treatment options. In this study, using immunohistochemistry and Western blot, we demonstrated that the expression of leukemia inhibitory factor (LIF), Janus kinase 1 (JAK1), and signal transducer and activator of transcription 3 (STAT3) was increased, while that of suppressor of cytokine signaling 1 (SOCS1) was decreased in RIF patients. Growth hormone (GH) administration proved to have positive effects on embryo implantation in RIF patients, but the action mechanism of GH has not been elucidated yet. To this aim, we studied the effects of GH on the proliferation in vitro of endometrial adenocarcinoma Ishikawa cells. GH stimulated the expression of LIF and SOCS1, and through SOCS1 inhibits the expression of phosphorylated STAT3, and finally inhibits the occurrence of RIF. Excessive phosphorylation of STAT can lead to decreased endometrial receptivity and abnormal embryo implantation. We also examined the effects of LIF overexpression and an LIF inhibitor (EC330) on the JAK/STAT pathway. LIF promoted cell proliferation, and the up-regulation of LIF increased the expression of SOCS1 and JAK1/STAT3 pathway-related genes in Ishikawa cells. As GH can inhibit the JAK1/STAT3 pathway through LIF, we hypothesize that upregulating SOCS1 may be a potential approach to treat RIF at the molecular level. GH can inhibit the JAK1/STAT3 pathway through LIF, up-regulating SOCS1 to treat RIF at the molecular level.
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CRYSTAL GROWTH & DESIGN
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2022
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IF 4.01
Lu Liu,Yue-Ming Yu,Fan-Zhi Bu,Yan-Tuan Li,Cui-Wei Yan,Zhi-Yong Wu
To give full prominence to the superiority of phenolic acid nutraceuticals syringic acid (SYRA) and gallic acid (GALA) in perfection of in vitro and in vivo performances for cardiotonic drug milrinone (MLN), and ulteriorly attain novice insights in the development of cardiotonic drug-nutraceutical cocrystals, two new solid forms of MLN including one cocrystal with SYRA, and one cocrystal hydrate with GALA─namely, MLN-SYRA (1) and MLN-GALA- H2O (2)─are first assembled and thoroughly characterized. The single-crystal X-ray diffraction showcases that both cocrystal 1 and cocrystal hydrate 2 have three-dimensional supramolecular structures controlled by hydrogen-bonding and aromatic packing interactions, where the hydrogen-bonding dimers of MLN itself still remain in 1 but are broken in 2, forming hydrophobic cavities in the former and hydrophilic ones in the latter. Such structural characteristics and aggregation motifs, because of the introduction of different phenolic acids in the crystalline lattices, have important influence on the improvements of dissolvability and permeability for the two crystalline forms, and in accordance with the theoretical studies. Intriguingly, the optimized in vitro biopharmaceutical properties can be effectually transformed to satisfactory in vivo pharmacokinetic peculiarity, performing the increased bioavailability and extended half-life. Thus, the current research not only offers promising solid-state forms for MLN with development foregrounds, but opens up new applications for nutraceutical phenolic acids in cardiotonic pharmaceutical cocrystals.
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