ANALYTICAL CHEMISTRY
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2026
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IF 7.3
Qingfei He,Lingjun Cheng,Yuanyuan Yang,Zhiyan Zou,Canping Su,Xuan Shi,Jiaming Chen,Weirong Huang,Zhenyu Lin,Guolin Hong
Gold nanoclusters (AuNCs) are emerging electrochemiluminescence (ECL) emitters with unique luminescence and high stability. However, conventional AuNC-based ECL systems, which rely on single-signal regulation, often exhibit weak signal responses and limited stability. To address the challenge of detecting extremely low concentrations of the Alzheimer’s disease (AD) blood biomarker Aβ40, this study presents a novel ECL-based biosensing platform that leverages dual-signal regulation of AuNCs via glutathione (GSH) and H2O2 serving as ECL quenchers. Based on the peroxidase-like activity of the Aβ40–hemin complex, this system restores the ECL signal by catalyzing the consumption reaction between GSH and H2O2. Using this mechanism, a dual-regulation ECL platform was developed, demonstrating a linear detection range of 0.001–100 ng/mL for Aβ40 with a detection limit of 0.18 pg/mL (S/N = 3). This approach not only offers high sensitivity and specificity but also reduces the complexity of surface modification steps. Overall, this study advances ECL technology through dual regulation and provides a sensitive, practical, efficient, and reliable analytical method for early AD screening.
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INORGANIC CHEMISTRY
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2024
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IF 4.3
Xueting Zhang,Cecile Genevois,Cheng Li,Xiaoyan Yang,Michael J. Pitcher,Mathieu Allix,Xiaojun Kuang,Alberto J. Fernández-Carrión
La2Mo2O9 is acknowledged as an exceptional oxide ion conductor. It undergoes a reversible phase transition around 580 °C from the nonconductive low-temperature monoclinic α-La2Mo2O9 phase to the highly conductive high-temperature cubic β-La2Mo2O9 phase. In addition, La2Mo2O9 demonstrates complex chemistry under reducing conditions. This study reports, for the first time, the stabilization at ambient temperature of a novel cubic phase through a topotactic reduction of α-La2Mo2O9 employing CaH2. This phase contains approximately ∼3 atom % oxygen vacancies relative to the nominal composition (La2Mo2O8.68(1)). The cubic symmetry is associated with a static distribution of these vacancies, in contrast to the dynamic distribution observed in the high-temperature cubic β-La2Mo2O9 phase reported previously. Additionally, the material exhibits mixed-ion-electronic conduction, which expands its potential use in applications requiring both ionic and electronic transport.
研究领域:
材料科学
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固体化学
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离子学
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功能材料
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电陶瓷
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Lixi Cai,Yanqi Gao,Yunmeng Chu,Yuanqing Lin,Lixing Liu,Guangya Zhang
Tailoring magnetic nanocarriers with tunable properties is of great significance for the development of multifunctional candidate materials in numerous fields. Herein, we report a one-pot biomimetic silicification-based method for the synthesis of silica-coated magnetic nanoparticles. The synthesis process was mild, low cost, and highly efficient, which took only about 21 min compared with 4.5–120 h in other literature. Then, the carriers had been characterized by VSM, SEM, TEM , XRD, FT-IR, and EDS to confirm their function. To evaluate the usefulness of the carriers, they were adopted to couple the purification and immobilization of β-1,3-xylanase from the cell lysate in a single step with high immobilization yield (92.8 %) and high activity recovery (82.4 %). The immobilized enzyme also retained 58.4 % of the initial activity after 10 cycles and displayed good storage properties, and improved thermal stability , which would be promising in algae biomass bioconversion as well as other diverse applications.
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ULTRASONICS SONOCHEMISTRY
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2022
·
IF 9.336
Xia Ma,Lin Dong,Yan He,Shiwen Chen
Yeast β-glucan (YG) possess an extensive range of biological activities, such as the inhibition of oxidation, but the poor water solubility of macromolecular YG limits its application. In this study, through the combined degradation of ultrasonic waves and H 2 O 2 , and the optimization of the main process parameters for solubilizing YG by response surface methodology (RSM), a new product of YG UH was generated. The molecular weight, structural characteristics and degradation kinetics before and after solubilization were evaluated. The results showed that the optimal solubilization conditions were reaction time: 4 h, ultrasonic power: 3 W/mL, H 2 O 2 concentration: 24 %. Under these conditions, ultrasound-assisted H 2 O 2 increased the solubility (from 13.60 % to 70.00 %) and reduced molecular weight (from 6.73 × 10 6 Da to 1.22 × 10 6 Da). Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), Congo red (CR), scanning electron microscopy (SEM) revealed that ultrasound-assisted H 2 O 2 increased the conformation's flexibility greatly, without changing the main structure of YG. More importantly, solubilization of YG improved free radical scavenging activity with YG UH exhibiting the highest levels of DPPH and ABTS + free radical scavenging activity. These results revealed that ultrasound-assisted H 2 O 2 degradation could be a suitable way to increase the solubility of YG for producing value-added YG.
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JOURNAL OF MEMBRANE SCIENCE
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2023
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IF 9.5
Huange Zhen,Mengyuan Wu,Zhijie Yuan,Zhibo Qi,Yingshuang Meng,Xihong Zu,Dan Liu,Gaohong He,Xiaobin Jiang
Nanofiltration (NF) is a promising technique to achieve sustainable and efficient separation of antibiotics with high industrial value. In this work, carboxymethyl- β -cyclodextrin (CM- β -CD) modified nanofiltration membrane was fabricated through interfacial polymerization (IP) for high concentration cephalexin solution separation, which is a classic antibiotic pharmaceutical wastewater. In principle, CM- β -CD can slow down the diffusion of amine monomers via electrostatic attraction, steric effect, hydrogen bonding and adsorption of the piperazine (PIP) monomer onto the hydrophilic surface, which renders amine monomer the high local concentration. The fabricated NF membranes possessed a larger proportion of dense primary layer on the surface. In addition, the accumulation of CM- β -CD between the substrate membrane and polyamide (PA) layer played the role as a barrier with specific cavity structure and bulk carboxylate to enhance the rejection of cephalexin. The cavity structure and stacking gap of CM-β-CD simultaneously rendered the membrane interlayer highly permeable. The optimal CM- β -CD modified membrane exhibited high rejection of cephalexin (94.7%) and water permeability of 122.4 L m −2 h −1 MPa −1 when the concentration of cephalexin was 3473.9 ppm (10 mmol/L). This work furnished an available way to prepare a novel nanofiltration membrane with efficient separation performance for relevant pharmaceutical production wastewater.
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Food Science and Human Wellness
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2022
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IF 8.022
Cheng Li,Yang Tian,Jianli Han,Yu Lu,Meiyi Zou,Yue Jia,Chengjian Wang,Linjuan Huang,Zhongfu Wang
β -Conglycinin is one of the major allergens existed in soybean. N -Glycans attached to the β -conglycinin influenced the immunoreactivity and antigen presenting efficiency of β -conglycinin. In this study, we described a new method used to release and collect the N -glycans from β -conglycinin, and the N -glycans existed in linear epitopes of β -conglycinin were identified. Glycopeptides hydrolyzed from β -conglycinin were purified by cotton hydrophilic chromatography. Trifluoromethylsulfonic acid was then used to release glycans from glycopeptides, and new glycopeptides containing one single N -acetyl- D -glucosamine (GlcNAc) moiety were then utilized for mass spectrometry. Five glycosylation sites (Asn-199, Asn-455, Asn-215, Asn-489 and Asn-326) and 22 kinds of glycopeptides were identified. It is noteworthy that the peptide VVN # ATSNL (where # represents for the glycosylation site) was analyzed to be both glycopeptide and linear epitope. Our results provided a new method for the N -glycoform analysis of food allergens, and laid a foundation for understanding the relationship between glycosylation and food allergy.
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NEUROGASTROENTEROLOGY AND MOTILITY
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2022
·Vol. 35
·期 2
·e14488
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IF 3.96
Mengjuan Lin,Guiying Hu,Baoping Yu
Background Hydrogen sulfide (H 2 S), an important endogenous gasotransmitter, is involved in the modulation of gastrointestinal motility, but whether it mediates the intestinal dysmotility in irritable bowel syndrome (IBS) is not known. This study explored the significance of cystathionine-β-synthase (CBS)/H 2 S signaling in stress-induced colonic dysmotility. Methods A rat model of IBS was established using chronic water avoidance stress (WAS). Colonic pathological alterations were detected histologically. Intestinal motility was determined by intestinal transit time (ITT) and fecal water content (FWC). Visceral sensitivity was assessed using the visceromotor response (VMR) to colorectal distension (CRD). Real-time PCR, Western blotting, and immunostaining were performed to identify the expression of CBS in the colon. The contractions of distal colon were studied in an organ bath system and H 2 S content was measured by ELISA. The effects of SAM, a selective CBS activator, on colonic dysmotility were examined. MEK1 was tested as a potential upstream effector of CBS/H 2 S loss. Key Results After 10 days of WAS, the ITT was decreased and FWC was increased, and the VMR magnitude in response to CRD was enhanced. The colonic CBS expression and H 2 S levels were significantly declined in WAS-exposed rats, and the density of CBS-positive enteric neurons in the myenteric plexus in WAS-treated rats was lower than that in controls. SAM treatment relieved WAS-induced colonic hypermotility via increased H 2 S production. AZD6244, a selective inhibitor of MEK1, partially reversed CBS downregulation and colonic hypermotility in WAS-treated rats. Conclusions & Inferences Decreased CBS/H 2 S signaling through increased MEK1 signaling might be important in the pathogenesis of chronic stress-induced colonic hypermotility. SAM could be administered for disorders associated with intestinal hypermotility.
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Separations
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2023
·Vol. 10
·期 2
·111
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IF 2.6
Zexing Xie,Meiying Xie,Tingting Tang,Fan Yang,Liyan Xue,Zhengming Jiang
6Li and7Li are strategic resources. Because Li+ions have no outermost electrons and the radii of6Li and7Li differ by only one neutron, the separation of the naturally stable isotopes of Li, especially by solvent extraction, is recognized as a difficult problem worldwide. Therefore, in this paper, an advancedβ-diketone-driven deep eutectic solvent (DES) extraction system containing 2-thenoyltrifluoroacetone (HTTA) and tri-n-octyl phosphine oxide (TOPO) is introduced to the extraction and separation of6Li+and7Li+ions. Compared with those of reported HTTA extraction systems and crown ether extraction systems, the separation coefficient (β7Li/6Li) of theβ-diketone-driven DES extraction system can reach the best value of 1.068, which is now the highest knownβ-value reported in the extraction system. From the intramolecular hydrogen bond of HTTA to the intermolecular hydrogen bond of DES, the bond energy increases by 47.8%. Because the active site of the proton in DES provides a higher energy barrier for the separation of7Li, theβ7Li/6Liis significantly increased. The extractions were characterized by spectrum, using1H nuclear magnetic resonance (NMR) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and X-ray photoelectron spectroscopy (XPS). The mechanism was determined on the basis of the reaction kinetics and density functional theory (DFT). The DES extractant shows excellent cycle performance with regard to stripping and reusability. In conclusion, the highly efficient, economical, and stableβ-diketone-driven DES extraction system can be used for the separation of naturally stable Li isotopes, which provides good industrial application prospects.Keywords:β-diketone;Lithium-7;separation of isotopes;deep eutectic solvent (DES);solvent extraction
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Journal of Food Measurement and Characterization
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2022
·Vol. 16
·期 2
·1383-1394
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IF 3.006
Zhang Daojiu,Zhang Yujie,Gao Yeying,Lin Yiying,Ding Chao,Zhang Jiwei,Liu Qingdai
In the present study, garlic essential oil (GEO) was embedded in lecithin (LT) and β -sitosterol ( β -S). Liposomes (L-S-G) were then prepared by injecting ethanol injection to improve bioavailability of GEO (51%) andthe particle size, encapsulation efficiency, and stability of L-S-G were analyzed. The stability of L-S-G samples under different storage conditions such as light and dark was also evaluated. Results of the present study showed that the encapsulation efficiency of the liposomes was 81% and the average particle size of the embedded L-S-G was 375.8 nm. The particle size of liposomes stored for 15 days under light and dark conditions were 529 nm and 473 nm, respectively. In addition, the findings from the gastrointestinal experiment in the current study showed that the bioavailability of L-S-G was 51%. The long-term bacteriostatic experiments of the present study revealed that the garlic essence liposomes effectively inhibited growth of Listeria monocytogenes in beef, chicken, and pork as compared with garlic essential oil. Graphical abstract
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Catalysis Science & Technology
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2022
·Vol. 12
·期 8
·2659-2669
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IF 6.177
Maoqi Li,Jian Wu,Guoliang Shen
Photocatalytic water splitting for hydrogen production and water pollutant degradation is a frontier topic to solve energy and environmental problems. In particular, applying electric and magnetic fields on photocatalysts is the most promising solution to improve their catalytic activity. In this work, bifunctional catalyst PDDA-coated β-Fe2O3 nanoclusters (β-Fe2O3@PDDA) have been successfully synthesized by a simple one-pot method at 200 °C. β-Fe2O3@PDDA exhibits excellent photoelectrocatalytic (PEC) activity for the oxygen evolution reaction (OER) with an overpotential of 300 mV, Tafel slope of 45 mV dec−1 and good catalytic stability. Moreover, β-Fe2O3@PDDA exhibited a good magnetic field-enhanced photocatalytic (MF-PC) RhB degradation activity under artificial simulated sunlight; the photocatalytic efficiency can be improved by 44% under an external magnetic field than that without a magnetic field. The surface PDDA-coated β-Fe2O3 can be stably dispersed in water for more than 10 days without agglomeration, which is of great significance for the MF-PC degradation of water pollutants in practical applications. The high catalytic activity for MF-PC is attributed to the magnetic field-suppressed electron–hole recombination and the spin-polarized properties of β-Fe2O3 in photocatalysis. Our study provides a new strategy for the preparation of highly dispersed β-Fe2O3 nanoclusters under mild conditions and the development of ultrastable organic/inorganic composite photocatalysts. This opens up a new avenue for developing electric and magnetic field-enhanced photocatalysts for use in clean energy and environmental control.
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SENSORS
·
2022
·Vol. 22
·期 5
·1912
·
IF 3.847
Lanlan Bai,Yun Gao,Jiajia Wang,Tuerxunnayi Aili,Zhenhong Jia,Xiaoyi Lv,Xiaohui Huang,Jie Yang
In this paper, carbon quantum dot-labelled β-lactoglobulin antibodies were used for refractive index magnification, and β-lactoglobulin was detected by angle spectroscopy. In this method, the detection light is provided by a He-Ne laser whose central wavelength is the same as that of the porous silicon microcavity device, and the light source was changed to a parallel beam to illuminate the porous silicon microcavity’ surface by collimating beam expansion, and the reflected light was received on the porous silicon microcavity’ surface by a detector. The angle corresponding to the smallest luminous intensity before and after the onset of immune response was measured by a detector for different concentrations of β-lactoglobulin antigen and carbon quantum dot-labelled β-lactoglobulin antibodies, and the relationship between the variation in angle before and after the immune response was obtained for different concentrations of the β-lactoglobulin antigen. The results of the experiment present that the angle variations changed linearly with increasing β-lactoglobulin antigen concentration before and after the immune response. The limit of detection of β-lactoglobulin by this method was 0.73 μg/L, indicating that the method can be used to detect β-lactoglobulin quickly and conveniently at low cost.Keywords:porous silicon microcavity;carbon quantum dots;angular spectrum
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SENSORS AND ACTUATORS A-PHYSICAL
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2022
·
IF 4.291
Jing Li,Guoyun Zhou,Yan Hong,Wei He,Shouxu Wang,Yuanming Chen,Chong Wang,Yao Tang,Yukai Sun,Yongkang Zhu
Wearable electronic devices with biocompatibility and stability to monitor the practical human physiological states are urgently expected for some intensive disease recovery or healthy fitness improvement. In this work, a highly sensitive piezoelectric sensor with the aid of polythiophene (PT) promoting polyvinylidene Fluoride (PVDF) from α-phase to β-phase was designed. Electric-sensitivity of 4.961 V/N is obtained at the low-pressure and 0.09 N periodic pressure positively produces considerable output open circuit voltage. It is proved that PT facilitates PVDF to accumulate charge carriers at their interfaces, resulting in interface polarization and thus extremely piezoelectric sensitivity. Reliability test suggested the sensor stays stable performance after a long-term (~2.6% decline after 5000 cycles) repetitive deformation. Through the practices of our assembled wearable sensor demonstrating its powerful ability in recording human joint movement and breathing, which also shows that it is very promising in online movement monitoring of personal health.
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