ORAL DISEASES
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2024
·
IF 2.9
Qin Jiang,Fang Wang,Gang Zhou
Objective Oral lichen planus (OLP) is a chronic inflammatory disease characterized by a dense T-cell infiltration and the degeneration of basal keratinocytes. The potential functions of mucosal associated invariant T (MAIT) cells in OLP have been analyzed in our previous study. Keratinocytes under proinflammatory conditions have been demonstrated to activate T cells. This study was aimed to investigate how keratinocytes stimulate MAIT cells in OLP, and to explore the role of activated MAIT cells on keratinocytes. Methods and Results Increased MAIT cells and higher activation marker CD69 were detected in OLP lesions by flow cytometry. The enhanced expression of MHC class I-like molecule (MR1) required for MAIT cell activation in the epithelial layer of OLP lesions was determined by immunohistochemistry. Keratinocytes treated by 5-A-RU prodrug and lipopolysaccharide, respectively, exhibited higher expression of MR1 and secretion of IL-18. In direct coculture systems consisting of keratinocytes and peripheral blood mononuclear cells, both 5-A-RU prodrug-pretreated keratinocytes and lipopolysaccharide-pretreated keratinocytes activated MAIT cells to secrete granzyme B, contributing to elevated keratinocyte apoptosis. Conclusions Keratinocytes were capable to activate MAIT cells via MR1 and cytokines in OLP, and granzyme B produced by activated MAIT cells intensified keratinocyte apoptosis, engaging in the pathogenesis of OLP.
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FIBERS AND POLYMERS
·
2018
·Vol. 19
·期 8
·1611-1617
·
IF 1.353
Wang Cuie,Wang Ning,Liu Xinhua,Wan Peng,He Xinwei,Shang Yongjia
We propose a green synthesis of benzopyran compound based on immobilized lipase. Firstly, by using a combined electrospun membrane and biocompatible chitosan, we show that the chitosan-functionalized electrospun PMA-co-PAA fibrous membrane can provide effective anchoring sites for immobilization of the Candida antarctica lipase B (CALB), and a maximum loading amount of CALB (152 mg/g) can be achieved. Then, the resulting membrane displays enhanced stability in catalyzing Knoevenagel condensation reaction of salicylaldehyde with active methylene compound in mixed aqueousorganic media. The catalytic activity and stability of the CALB on chitosan-modified fiber is better than its non-immobilized counterpart. Furthermore, this immobilize CALB catalyst still retained about 45 % of the initial activity after 5 cycles, which potentially had implications for the design of “green” enzyme catalyst for sustainable organic synthesis.
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International Journal of Environmental Research and Public Health
·
2022
·Vol. 19
·期 14
·8683
·
IF 4.614
Xiang Guo,Lei Rao,Zhenyu Shi
Adsorption can quickly remove pollutants in water, while photocatalysis can effectively decompose organic matter. B-TiO2/g-C3N4ternary composite photocatalytic materials were prepared by molten method, and their adsorption–degradation capability under visible light conditions was discussed. The morphology of the B-TiO2/g-C3N4materials was inspected by SEM, TEM, BET, and EDS, and the results showed that close interfacial connections between TiO2and g-C3N4, which are favorable for charge transfer between these two semiconductors, formed heterojunctions with suitable band structure which was contributed by the molten B2O3. Meanwhile, the molten B2O3effectively increased the specific surface area of TiO2/C3N4materials, thereby increasing the active sites and reducing the recombination of photogenerated electron–hole pairs and improving the photocatalytic degradation abilities of TiO2and g-C3N4. Elsewhere, the crystal structure analysis (XRD, XPS, FTIR) results indicated that the polar -B=O bond formed a new structure with TiO2and g-C3N4, which is not only beneficial for inhibiting the recombination of electron holes but also improving the photocatalytic activity. By removal experiment, the adsorption and degradation performances of B-TiO2/g-C3N4composite material were found to be 8.5 times and 3.4 times higher than that of g-C3N4. Above all, this study prepared a material for removing water pollutants with high efficiency and provides theoretical support and experimental basis for the research on the synergistic removal of pollutants by adsorption and photocatalysis.Keywords:adsorption;degradation;B2O3;TiO2/C3N4;molten
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INORGANIC CHEMISTRY
·
2021
·
IF 5.165
Weiwei Cao,Xiaoyan Yang,Cécile Genevois,Mathieu Allix,Xiaojun Kuang
The new 8-layer twinned hexagonal solid solution Ba8Cr4–xTa4+0.6xO24 (x = 0.0–3.0) was isolated through the aliovalent substitution of Ta5+ for Cr3+ in Ba2CrTaO6, showing the widest B-site vacancy content range among the 8-layer twinned hexagonal perovskites. Ba8Cr4–xTa4+0.6xO24 forms a simple 8-layer hexagonal perovskite structure within 0.0 ≤ x < 2.4 and a tripled 8-layer hexagonal perovskite superstructure within 2.4 ≤ x ≤ 3.0. The latter shows expanded a and b axes by times in comparison to the simple 8-layer hexagonal perovskite structure owing to the partial face-sharing octahedral (FSO) B cation ordering along the ab plane. The B-cation and vacancy distributions in the tripled superstructure were characterized by neutron and X-ray powder diffraction and further confirmed by a scanning transmission electron microscopy–high angle annular dark field imaging and intensity profile analysis. The formation of 8-layer twinned hexagonal perovskites Ba8Cr4–xTa4+0.6xO24 in an extended solid solution range can be attributed to the presence of both covalent B–B and B–O–B bonding and B-site vacancies in the FSO sites. This work provides an effective way of combining covalent B–B and B–O–B bonding and vacancy creation as well as the cationic ordering in the FSO sites to reduce electrostatic repulsion, which could further enable the stabilization of new hexagonal perovskite compounds.
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BIOMEDICINE & PHARMACOTHERAPY
·
2023
·
IF 7.5
Chunxia Hu,Jinyong Zhang,Ruiqin Cui,Shiyi Liu,Ying Huang,Huan Zeng,Shumin Cheng,Guibao Zhou,Jingli Li,Longqin Sun,Yan Zhao,Xiao Wang,Jianhua Liu,Quanming Zou,Wei Huang
Given the important role of polymyxin B (PB) in the treatment of drug-resistant Gram-negative bacterial infections, the emergence of PB resistance poses a serious threat to public health. Adjuvant development is a supplementary strategy that can compensate for the lack of novel antibiotics by protecting PB. In this study, we found a small molecule named Lyb24 that showed weak antibacterial activity (minimum inhibitory concentration ≥ 10 μg/ml) but potentiated and revitalized the efficacy of PB against Gram-negative pathogens, including mcr-1 - and mgrB -deletion-mediated PB-resistant strains. Our results showed that Lyb24 inhibits the translational levels of genes associated with the modification of lipid A. In addition, Lyb24 increases the permeability, disrupts the integrity and induces the depolarization of the membrane. We further found that both Lyb24 and PB could directly bind to AzoR and inhibit its activity. Structural analysis showed that Lyb24 binds to the isoalloxazine ring of flavin mononucleotide (FMN) through pi-pi stacking and loop η4 of AzoR. A pneumonia model was used to confirm that the activity against clinical PB-resistant Klebsiella pneumoniae was enhanced due to Lyb24 on PB. In conclusion, we provide a potential therapeutic regimen by combining Lyb24 and PB to treat Gram-negative-resistant bacterial infections. Our findings not only explain the synergistic effect of Lyb24, but also expand our knowledge on the mechanism of action of PB.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Sikai Wu,Jing Shi,Jixiang Chen,Deyu Hu,Liansheng Zang,Baoan Song
A series of novel 6-sulfonyl-1,2,4-triazolo[3,4-b][1,3,4]thiadiazole derivatives were designed and synthesized. CoMFA models were established to analyze the quantitative structure–activity relationships on the basis of the EC50 values of the compounds. The models were used to design and synthesize compounds 32 and 33 with higher activities. The EC50 values of compound 33 against Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) were 0.59 and 1.63 mg/L, respectively, which were higher than those of thiodiazole copper (90.43 and 97.93 mg/L) and bismerthiazol (68.37 and 75.59 mg/L). Moreover, protective activities of compound 33 against bacterial leaf streak (BLS) and bacterial leaf blight (BLB) were 49.65% and 49.42%, respectively, which were superior to those of thiodiazole copper (44.28% and 41.51%) and bismerthiazol (38.89% and 40.09%). Protective activity of compound 33 against BLS was closely related to the improvement of defense-related enzyme activities, chlorophyll content, and photosynthesis activation. This is consistent with the upregulated expression of defense responses and photosynthesis-related proteins.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2015
·Vol. 26
·期 9
·1-12
·
IF 2.587
Zeng Xiaolong,Zhou Xiaoyan,Li Mengyi,Wang Changyong,Xu Jiake,Ma Dong,Xue Wei
Bioreducible polymers have appeared as the ideal drug carriers for tumor therapy due to their properties of high stability in extracellular circulation and rapid drug release in intracellular reducing environment. Recently, the diselenide bond has emerged as a new reduction-sensitive linkage. In this work, the amphiphilic poly(ethylene glycol)- b -poly( l -lactide) containing diselenide bond has been synthesized and used to load anti-tumor drug, docetaxel (DTX), to form the redox micelles. It was found that the redox micelles showed a rapid response to glutataione (GSH), which resulted in a fast release of DTX in the presence of GSH. In contrast, <40 % of DTX was released from the micelles within 72 h under the normal condition (absence of GSH). The DTX-loaded redox micelles showed the significant inhibition effect to MCF-7 cells, and the cytotoxicity was dependent on the intracellular GSH concentrations. Moreover, considering the potentially clinical applications of the micelles through intravenous injection, the blood compatibility was also studied by the hemolysis analysis, activated partial thromboplastin time, prothrombin time and thromboelastography assays. These results confirmed that the redox micelles showed good blood safety, suggesting a potential application in tumor therapy.
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DYES AND PIGMENTS
·
2016
·
IF 4.055
Xing Qian,Rucai Yan,Yongjie Hang,Yuezhen Lv,Lixin Zheng,Chong Xu,Linxi Hou
Four new metal-free organic dyes QX15 – 18 based on indeno[1,2- b ]indole have been successfully designed and synthesized. These D–π–A type dyes consist of an indeno[1,2- b ]indole donor and a benzene or thiophene π-bridge. In an attempt to tune their photoelectric properties, the effects of different acceptor groups including cyanoacrylic acid, rhodanine-3-acetic acid, and 2-(1,1-dicyanomethylene)rhodanine (DCRD) have been investigated. The dye QX15 with a cyanoacrylic acid acceptor exhibited a highest V oc of 813 mV and a high J sc of 11.0 mA cm −2 , generating a highest power conversion efficiency of 6.29%. Meanwhile, the dye QX18 with a DCRD acceptor and a thiophene π-bridge showed a significantly improved J sc of 11.9 mA cm −2 , which could be attributed to its red-shifted absorption spectra and high molar absorption coefficient.
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TALANTA
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2021
·
IF 6.057
Hao Yang,Danke Xu
As an important kind of environmental endocrine disruptors , 17 β -Estradiol (E2) plays a major role in affecting the growth of human including sexual characters, pregnancy system, etc. In the modern society, with the threat of abuse in breeding, it is imperative to design sensitive methods for detecting low concentration of E2 in environment. In this work, we constructed a highly sensitive and simple fluorescent aptasenor for detecting E2 via amplification of hybridization chain reaction (HCR) and horseradish peroxidase (HRP). Through the competitions between complementary strand (cmDNA) and E2 to E2 aptamer modified on magnetic beads, the unbound cmDNA would be collected and captured by polystyrene microspheres to induce HCR which brought abundant biotin sites. Subsequently, benefit from the excellent catalytic performance of streptavidin-horseradish peroxidase (SA-HRP), the highly sensitive fluorescence signals could be obtained in low concentration of E2. Under the optimal conditions, the prospered method for E2 detection was shown a good liner range from 1 to 100 pg/mL, with the lower detecting limit of 0.2 pg/mL compared with previous work. In addition, the recovery rates tested in the real samples of milk and water were 99.20%–108.06% and 91.07%–106.13%. In all, the assay may provide a perspective way for highly sensitive detection for various contaminants in the real samples.
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高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
Hu Linzhen,Zhu Hucheng,Li Lei,Huang Jinfeng,Sun Weiguang,Liu Junjun,Li Hua,Luo Zengwei,Wang Jianping,Xue Yongbo,Zhang Yu,Zhang Yonghui
Two pairs of new enantiomers with unusual 5,5-spiroketal cores, termed (±)-japonones A and B [(±)- 1 and (±)- 2 ], were obtained from Hypericum japonicum Thunb. The absolute configurations of (±)- 1 and (±)- 2 were characterized by extensive analyses of spectroscopic data and calculated electronic circular dichroism (ECD) spectra, the application of modified Mosher’s methods and the assistance of quantum chemical predictions (QCP) of 13 C NMR chemical shifts. Among these metabolites, (+)- 1 exhibited some inhibitory activity on Kaposi’s sarcoma associated herpesvirus (KSHV). Virtual screening of (±)- 1 and (±)- 2 were conducted using the Surflex-Dock module in the Sybyl software and (+)- 1 exhibited ability to bind with ERK to form key interactions with residues Lys52, Pro56, Ile101, Asp165, Gly167 and Val99.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2012
·
IF 2.625
Chi Ma,Di Pang,Zuochun Xiong,Wei Bai,Chengdong Xiong
Control of cellular responses is crucial for the use of electrospun membranes in biomedical applications, including tissue engineering or biomedical devices. However, it is still unclear whether adhesion and proliferation of fibroblasts is stimulated or inhibited on polyethylene glycol (PEG)-modified electrospun membranes. In this study, poly( L -lactide-co-glycolide) (PLLGA)–PEG copolymer and pure PEG were blended with PLLGA, and then electrospun onto nonwoven membranes. The effects of blending of PLLGA–PEG or pure PEG on the adsorption of proteins, and further on the adhesion and proliferation of L929 fibroblasts on the electrospun membranes were investigated. Addition of PLLGA–PEG or PEG significantly improved the hydrophilicity of the electrospun membranes. Pure PEG had no obvious effects on the growth of L929 fibroblasts; in contrast, PLLGA–PEG significantly inhibited the adsorption of proteins and the proliferations of the cells on the electrospun membranes. In response to diminished protein adsorption, mRNA expression of genes related to cell adhesion and migration was up-regulated. The limited effects of pure PEG were probably caused by its preferential dissolution, whereas membrane-confined PLLGA–PEG displayed excellent performance on the inhibition of protein adsorption and cell proliferation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:2897–2904, 2012.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Yibo Gao,Chengxin Xu,Xiaoyan Yang,Wen Yin,Shulin Liu,Xiaojun Kuang
Twinned hexagonal perovskite Ba 8 ZnTa 6 O 24 exhibits partially B-cation order but exceptionally low microwave dielectric loss, surpassing those of its fully ordered shifted hexagonal perovskite counterparts, on which the underlying structural origin has not been fully understood. This study employs neutron pair distribution function (nPDF) analysis combined with reverse Monte Carlo (RMC) modeling to elucidate the local B-site cation and vacancy ordering in Ba 8 ZnTa 6 O 24 . Refinements against nPDF data, utilizing both small unit cell and large supercell (8 ×8 ×4) models, consistently confirm that corner-sharing octahedral (CSO) sites are exclusively fully occupied by Ta 5 + cations and face-sharing octahedral (FSO) sites are partially occupied by both Zn 2+ and Ta 5+ cations, accommodating the B-site vacancies. The large supercell RMC simulations confirm the specific B-site atomic and vacancy arrangement avoiding strong electrostatic repulsion by preventing high-charge Ta 5+ -Ta 5+ pairs in the FSO layers and establishes charge neutrality over short ranges, which consequently could account for the observed ultra-low dielectric loss. This work provides atomic-scale cation-ordering insights into the low microwave loss of twinned hexagonal perovskites, offering guidance for designing high-performance microwave dielectrics.
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