Microbiology Spectrum
·
2023
·Vol. 11
·期 6
·
IF 3.7
Minyu Wang,Zheng Wang,Duncan James Lessing,Min Guo,Weihua Chu
Fusobacterium nucleatum is a harmful bacterium that produces hydrogen sulfide by metabolizing sulfur-containing amino acids, both of which have been shown to promote/initiate the development of various cancers, such as colorectal cancer, liver cancer, as well as lung cancer. However, few studies have reported how F. nucleatum acts on gut microbiota, influences the autophagy process, and promotes the development of colorectal cancer through the metabolite hydrogen sulfide. It was found that HCT116 cells secreted more inflammatory factors and showed higher survival as well as migration rate when given a pathological concentration of hydrogen sulfide. Using cellular transcriptome sequencing, it could be seen that after giving HCT116 cells F. nucleatum or F. nucleatum with L-cysteine, many inflammation-related signaling pathways, including autophagic pathways, were elevated compared to the control group. In vivo, the mice in the model groups displayed cancer progression, and the expression of some of the autophagy-related genes, DRAM1, NBR1, ATG7, MAP1LC3A were also increased in mice given F. nucleatum or F. nucleatum with L-cysteine when compared to the untreated control mice. The number of certain probiotic bacteria and fungi in the mice given F. nucleatum or F. nucleatum and L-cysteine decreased significantly; in contrast, the number of potentially pathogenic bacteria and fungi increased. These findings suggest that F. nucleatum and its metabolite hydrogen sulfide can modulate intestinal microbiota and autophagy as well as promote the development and progression of colorectal cancer.
研究领域:
微生物学
·
癌症生物学
·
分子生物学
·
胃肠病学
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高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Chanjuan Xu,Bingxian Yang,Wei Zhu,Ximin Li,Jingkui Tian,Lin Zhang
The leaves of Linderae aggregate (LAL) has been used as a type of tea in China and other Southeast Asian countries. In this study, 11 polyphenols in LAL were clarified for the first time using multiple high-performance liquid chromatographic techniques. An optimal extracting method was developed through the comparison of the amount of quercetin-3- O -α- l -rhamnoside using a uniform design method. From the fingerprint liquid chromatography data, 11 common peaks in the 8 samples collected from April to November were semi-determined. The antioxidant capacities were examined using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay and the ferric reducing/antioxidant power assay. All 8 samples contained the same 11 polyphenols in similar ratios. Three samples, S2, S5 and S6 contained higher amount of quercetin-3- O -α- l -rhamnoside and were demonstrated to have stronger antioxidant capacities in both antioxidant assays. These results are critical in optimising harvest time and quality control of LAL.
研究领域:
植物化学
·
药理学
·
分析化学
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DOI
高影响因子
Journal of Sulfur Chemistry
·
2022
·
IF 2.325
Ling Jiang,Qingqing Lu,Qianqian Huang,Yue Cheng,Xiaohe Chu
In this paper, a simple and environment-friendly protocol was developed for the synthesis of arylsulfonyl 4H-pyrans via L-proline-catalyzed one-pot two-step reaction of aromatic aldehyde with phenylsulphonyl acetonitrile and dimedone under ball-milling conditions. Environmental acceptability, wide substrate scope, low cost and operational simplicity are the key features of this method.
研究领域:
有机化学
·
绿色化学
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DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2020
·
IF 2.627
Jiyuan Li,Mifen Cui,Zhuxiu Zhang,Xian Chen,Qing Liu,Zhaoyang Fei,Jihai Tang,Xu Qiao
Isooctane attracts great interest in recent years because of its promising potential as friendly-environmental gasoline, which is obtained by dimerization of isobutene with a hydrogenation step. Herein, a solid acid catalyst sulfated zirconia modified by ZnO was prepared. The oligomerization of isobutene had been investigated over ZrO 2 -SO 4 and ZnO( X )/ZrO 2 -SO 4 catalyst in order to find efficient catalysts for the production of isobutene oligomers . The presence of ZnO obviously enhanced the dimerization of isobutene and ZnO( X )/ZrO 2 -SO 4 exhibited the highest di-isobutene yield of 60%. Kinetic studies showed the higher trimerization-to-dimerization activation energy ratios of ZnO( X )/ZrO 2 -SO 4 than those of ZrO 2 -SO 4 from 353 to 393 K. In addition, reaction rate of dimerization was higher than trimerization over ZnO( X )/ZrO 2 -SO 4 . The high L/B ratio manifested the capability to enhance the selectivity of C 8 in isobutene dimerization. Furthermore, ZnO( X )/ZrO 2 -SO 4 exhibited stable conversion for the dimerization of isobutene.
研究领域:
化学工程
·
催化
·
石油化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 3
·1107-1116
·
IF 3.288
Yan Qiao,Yilian Li,Wenxian Li,Jinrong Bao,Yushan Zheng,Lina Feng,Yangyang Ma,Kuisuo Yang,Anping Wu,He Bai,Yunjiang Yang
In this paper, novel core–shell (CS) structure composites SiO2@ANA-Si–Eu and SiO2@ANA-Si–Eu–L (L = phen, TTA) were prepared by the condensation of 5-N-bis(amidopropyltriethoxysilyl) nicotinic acid (HOOCC5H4NN(CONH(CH2)3Si(OCH2CH3)3)2, abbreviated as ANA-Si) and a SiO2 microsphere with a diameter of 200 nm in the absence of surfactants. The as-synthesized CS structure composites possess good dispersion, uniform size and excellent luminescence properties. Furthermore, silica modified core–shell–shell (CSS) structure composites SiO2@ANA-Si–Eu@SiO2 and SiO2@ANA-Si–Eu–L@SiO2 were successfully prepared by a sol–gel method using CS structure composites as the core and amorphous silica as the shell. Compared with the CS structure composites, CSS structure composites exhibited a stronger luminescence intensity in the solid state. Furthermore, an improved luminescence stability property of the CSS structure composites was found in aqueous medium. CSS structure composites exhibited improved luminescence, high stability and good solubility, which provided potential for their wide use in advanced phosphors, molecular biomarkers and luminescent tracers.
研究领域:
材料科学
·
发光材料
·
无机化学
·
纳米复合材料
·
光学材料
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-12
·
IF 6.232
Dong Wenjuan,Wang Ruiping,Gong Xiaojuan,Liang Wenting,Fan Li,Song Shengmei,Dong Chuan
A far-red fluorescence “off-on” sensing strategy is described for sequential ratiometric determination of Cu 2+ and L-histidine (L-His) based on fluorescence resonance energy transfer (FRET) system. N,S,P co-doped carbon dots (N,S,P-CDs) and N-acetyl-L-cysteine functionalized gold nanoclusters (NAC-AuNCs) are used in the FRET system, which serve as energy donor and acceptor, respectively. After adding NAC-AuNCs into the solution of N,S,P-CDs, the fluorescence of N,S,P-CDs is effectively quenched, while the far-red fluorescence of NAC-AuNCs appears. Cu 2+ can decrease fluorescence of NAC-AuNCs, and then L-His can effectively recover the fluorescence of NAC-AuNCs. The possible reason is that the stronger affinity between Cu 2+ and L-His can pull Cu 2+ away from the surface of NAC-AuNCs. Through it all, the emission intensity of N,S,P-CDs remains nearly constant, so the ratio of fluorescence intensities at 485 and 625 nm exhibits a linear correlation to the Cu 2+ and L-His concentration, respectively. The sensing platform shows good selectivity towards Cu 2+ and L-His with a linear range of 0.65–26.58 μM and 3.13–56.25 μM and determination limits of 0.50 μM and 0.374 μM, respectively. The proposed method has been successfully used for Cu 2+ and L-His determination in real samples with satisfying results.
Graphical abstract
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DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2018
·Vol. 18
·期 1
·576-582
·
IF 1.354
Yu Yadong,Wu Na,Peng Gang,Li Tao,Jiang Ling,Huang He
Convenient and sensitive methods for the detection of α-ketoglutarate (α-KG) will be beneficial for biomedical diagnosis and microbial fermentation industry. In this work, reduced MoS2 nanoparticles (rMNs) were prepared by ultrasonication and electrochemical reduction.
The rMNs were then decorated with gold nanoparticles (Aunano) to fabricate rMNs-Aunano nanocomposite. The glass carbon electrode modified with rMNs-Aunano nanocomposite (rMNs-Aunano-GCE) showed excellent electro-catalytic oxidation toward NADH with
a satisfied reproducibility and exhibited linear response in the range of 38.46–214.29 μM. Moreover, the L-Glutamic dehydrogenase modified rMNs-Aunano-GCE exhibited a sensitive and fast response to α-KG, with a linear response range of 11.12–52.94 μM
and a detection limit of 6.25 μM. These findings will pave ways for the α-KG detection and the application of MoS2 or other two-dimensional materials in electrochemical sensing.
研究领域:
生物医学
·
微生物发酵工业
·
电化学传感
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yuanyuan Dong,Jinming Li,Lei Zhang
Epinephrine (EP) is a catecholamine neurotransmitter produced from l -tyrosine (L-TY), which usually coexists with each other in biological fluids. Moreover, their abnormal concentrations are related to multiple diseases, so sensitive simultaneous determination of EP and l -TY is important. Herein, we delicately designed 3D SnS@Co ,N- C hierarchical hollow microrod assembled from 2D SnS nanoplates vertically anchored on the MOF derived cobalt, nitrogen co-doped carbon rod (Co ,N- C) as electrode materials using one-step hydrothermal method, followed by thermal annealing in N 2 , and accompanying sequential phase transformation (2D SnS 2 nanoplates in-situ reduced to SnS phase). Obtained hierarchical hollow carbon microrod with beneficial heteroatom doped not only provided sufficient electroactive sites, but also increased the conductivity of the materials. As-fabricated strongly coupled 3D SnS@Co ,N- C microrod displayed enhanced electrochemical performance, offering highly sensitive sensing responses toward biomolecules EP and L-TY. Differential pulse voltammetry (DPV) study indicated that the sensor offered wide linear ranges of 0.01–300.0 and 0.01–250.0 μmol/L, and low detection limits of 3.3 and 3.1 nmol/L for EP and L-TY respectively. The constructed sensor was successfully applied to monitor simultaneously EP and L-TY in milk, human urine and serum samples with high accuracy and reliability.
研究领域:
材料科学
·
生物医学工程
·
分析化学
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DOI
高影响因子
Nano Today
·
2025
·
IF 10.9
Wei Hu,Jiaxi Xu,Mingchen Lv,Min Sun,Zhen Fan,Jianzhong Du
Immunotherapy offers enduring cancer treatment by activating the immune system to eliminate cancer cells, but faces challenges like tumor immune evasion and low immunogenicity. Chirality, crucial in biological interactions, remains underexplored for immune modulation. Inspired by the metal-binding domain of manganese superoxide dismutase, we develop a cyclic D/L peptide for chirality-driven immune activation. The cyclic L -Tyr- D -His- L -Asp- D -His (YhDh) peptide coordinates with Mn 2+ to self-assemble into nanoparticles, cloak with tumor cell membranes for homologous targeting. Compared to all-L or all-D peptides, YhDh nanoparticles promote costimulatory molecule expression on dendritic cells (DCs). Upon intravenous administration, they are oxidized at melanoma sites with tyrosinase overexpression, enabling photothermal-induced immunogenic cell death (ICD). Chiral peptides synergize with Mn 2+ -mediated cGAS-STING activation and ICD, enhancing DC maturation and T cell infiltration (4.36- and 2.88-fold), suppressing tumor metastasis while establishing immune memory. Such chiral cyclic peptides offer a novel strategy for immune activation by regulating peptide chirality.
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高影响因子
ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Hongjie Zong,Bo Wang,Guifei Li,Shifeng Yan,Kunxi Zhang,Yufeng Shou,Jingbo Yin
Currently, biodegradable hydrogels are one of the most promising materials in tissue engineering. From the perspective of clinical needs, hydrogels with high strength and minimally invasive implantation are preferred for the reconstruction of load-bearing tissues. In this work, a biodegradable, high-strength, and injectable hydrogel was developed by one-step photo-cross-linking of two biomacromolecules, polyethylene glycol acrylated poly(l-glutamic acid) (PLGA-APEG) and methacrylated gellan gum (GG-MA). The hydrogels, named as PLGA/GG hydrogels, exhibited high mechanical properties. The compression stress of the hydrogels was 0.53 MPa, and the fracture energy was 7.7 ± 0.2 kJ m–2. Meanwhile, the storage modulus could reach 44.0 ± 0.6 kPa. The hydrogel precursor solution loaded with adipose-derived stem cells (ASCs) was subcutaneously injected into C57BL/6 mice and then cross-linked via in situ transdermal irradiation, which demonstrated the excellent injectability and subcutaneous gelatinization of PLGA/GG hydrogels as cell carriers. Furthermore, three-dimensional encapsulation of ASCs in hydrogels after 7, 14, and 21 days showed outstanding cytocompatibility, and the viability of ASCs was up to 84.0 ± 1.7%. The PLGA/GG hydrogels exhibited ideal behaviors of degradation, with 60 ± 5% of hydrogels degraded in phosphate buffered solution (PBS) after 11 weeks. H&E staining demonstrated that the hydrogels degraded gradually after 6 weeks and supported tissue invasion without inflammatory reactions, which indicated the laudable biodegradability of hydrogels. Hence, the biodegradable and high-strength hydrogels with well-performed injectability and biocompatibility possessed high potential applications in tissue engineering, especially in load-bearing tissue regeneration.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2026
·
IF 9.5
Bing Liu,Haoming Zhong,Hina Gul,Hao Peng,Xiaolei Guo,Yu-Long Xie,Minhua Ai,Xiangwen Zhang,Ji-Jun Zou,Lun Pan
Biomass electrooxidation for coupled hydrogen production offers a promising route to overcome the sluggish kinetics and high energy cost of conventional water electrolysis. The electrocatalytic oxidation of L-ascorbic acid (AA) to high-value dehydroascorbic acid (DHA) is particularly attractive but requires efficient catalysts to facilitate the challenging two-step dehydrogenation process. Here, we report a FeRu-NC catalyst with excellent L-ascorbic acid oxidation reaction (AAOR) catalytic activity and selectivity. The FeRu-NC catalyst achieves a significantly lower overpotential of 10 mV at 10 mA•cm⁻² and delivers a high current density of 200 mA•cm⁻² at only 0.68 V vs. RHE, while maintaining stable operation for over 80 h. DHA is produced with high yield (~95%), selectivity (~97%), and Faradaic efficiency (~95%). XPS, XAFS studies and in-situ IR spectroscopy reveal that the introduction of trace Ru modulates the electronic structure of Fe single-atom sites, enhancing the adsorption of key intermediates and promoting the second dehydrogenation step, presenting a highperformance AAOR activity and high selectivity for DHA.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Guangai Sun,Shijie Guo,Yuanyuan Yao,Zeyu Lin,Hui Gao,Roland H. Stauber,Bin-Chun Li,Guo-Bin Ding
α-L-Rhamnosidase is a significant biocatalyst that specifically cleaves terminal α-L-rhamnose group from natural flavonoid diglycosides, enabling the biocatalytic production of high-value flavonoid glucosides like prunin from naringin. Compared to its precursor naringin, prunin exhibits superior solubility and bioavailability, making this enzymatic conversion commercially valuable for the food and pharmaceutical industry. However, the industrial application of free α-L-rhamnosidases faces significant challenges, including enzyme instability, difficulty in recovery, and unsatisfactory reusability. To address these issues, this study developed an innovative magnetically recoverable biocatalyst (Fe₃O₄-Rha) through covalent immobilization of recombinant α-L-rhamnosidase from human fecal metagenome onto Fe₃O₄ nanoparticles using EDC/NHS chemistry, and the immobilization parameters were systematically optimized. The successful preparation of Fe₃O₄-Rha was verified by TEM, FTIR, TGA, and SQUID analysis. Fe₃O₄-Rha retained the catalytic property of free Rha in terms of optimal pH and temperature but exhibited superior tolerance on organic solvent especially ethanol and isopropanol. Moreover, Fe₃O₄-Rha could effectively biotransform naringin to prunin and maintained 61.34% of initial activity after 5 cycles. In a scaled-up reaction system, Fe₃O₄-Rha also efficiently converted naringin into prunin and the complete conversion was achieved within 10 h. This work successfully developed a magnetically recoverable immobilized α-L-rhamnosidase system for the efficient and reusable biotransformation of naringin, offering a promising approach for the enzymatic modification of bioactive small molecules using biological macromolecular catalysts.
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PubMed
高影响因子
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