ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Hongtao Li,Yan Luo,Binbin Zhai,Helan Zhang,Yiping Tian,Yanyan Luo,Pei Wang,Yu Fang
A key challenge in the commercialization of surface-enhanced Raman scattering (SERS) technology is the scalable and controllable preparation of substrates that simultaneously achieve high sensitivity, uniform signal distribution, excellent reproducibility, and long-term stability. In this study, we report a flexible SERS substrate based on a porous, high-surface-energy nanofilm, designed to serve as a spatially confined template for the uniform growth of silver nanoparticles (AgNPs). This substrate exhibits outstanding SERS performance with an enhancement factor (EF) of 2.05 × 1010 and a detection limit (DL) as low as 1.74 × 10–15 M for rhodamine 6G (R6G). The preparation process is mild, highly reproducible (RSD = 8.53% over 60 consecutive batches), and scalable to 25 cm2 areas. Moreover, the substrate maintains its SERS activity for over three months within a vacuum bag at room temperature. Owing to its excellent adhesion and good flexibility, the substrate can adhere effectively to complex surfaces. The substrate was successfully applied to sensitive in situ detection of trace thiram residues on grape surfaces, achieving a detection limit (DL) of 8.51 × 10–10 g cm–2. Additionally, a wearable sensor was developed based on the substrate and used for the noninvasive monitoring of uric acid levels in human sweat, with a DL of 2.95 × 10–7 M. This work provides a practical strategy for constructing high-performance, scalable, and flexible SERS substrates, advancing their potential for real-time, on-site analysis.
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高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Willis Kwun Hei Ho,Qin Zhang,Fariza Zhorabe,Jiaxiang Yan,Yutian Gu,Shujun Wang,Changqing Yi,Yu Zhang,Mo Yang
Amyloid-β (Aβ) plaques are a key pathological hallmark of Alzheimer's disease (AD), highlighting the need for highly sensitive bioassays for Aβ detection to enable AD diagnosis. Here, we synthesized a buoyant plasmonic substrate composed of polyvinyl alcohol microbubbles (MBs) decorated with in situ-reduced gold nanoparticles (Au NPs). Benefiting from its inherent buoyancy and near-infrared plasmonic properties, the Au/MB substrate serves as an ideal platform for biomolecular sensing via the surface-enhanced Raman spectroscopy (SERS) technique. Compared to conventional flat SERS substrates, the three-dimensional (3D) curved surface of the Au/MB substrate significantly increases the effective sensing area, while its inherent buoyancy facilitates the efficient removal of unbound targets, thereby enhancing detection specificity. By functionalizing Au/MB substrates with copper ions (Cu2+) and 4-mercaptobenzoic acid (4-MBA), we achieved sensitive detection of AD-related Aβ proteins. In the presence of the target analyte, the interaction between Aβ proteins and Cu2+ induces molecular deformation and orientation changes in 4-MBA, leading to distinct spectral changes in the SERS signals. The results demonstrate that the developed Au/MB-based SERS sensor enables sensitive detection of Aβ1–40 oligomers with a sensitivity as low as 10−9 M. Therefore, this work not only establishes a foundational framework for designing buoyant plasmonic substrate-based SERS sensing platform but also paves the way for the quantitative detection of disease-associated protein biomarkers, contributing to advancements in AD diagnostics.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 7
·1-14
·
IF 5.3
Gao Yueming,Weng Guojun,Li Jianjun,Zhu Jian,Zhao Junwu
A flexible silver triangular nanoplate (Ag TNPs)@m-phenylenediamine (mPD)@polyacrylonitrile (PAN) skin patch based on electrospinning is fabricated and applied to detect human beta-defensin 2 (HBD-2) by using surface-enhanced Raman scattering (SERS). First, PAN was used as the base material to create a fibrous membrane through electrospinning. The fibers were then modified with Ag TNPs by mPD endowing positive charges, forming a flexible Ag TNPs@mPD@PAN substrate. The flexible substrate features advantages of simple preparation, low cost, and convenient portability, which are suitable for in situ rapid detection on irregular surfaces. The combination of the flexible Ag TNPs@mPD@PAN substrate with Ag TNPs probes enabled the specific detection of HBD-2. It demonstrated satisfactory sensitivity and reproducibility with a wide detection range of 100–10 ng/mL and a limit of detection (LOD) of 59.57 fg/mL. Eventually, the flexible substrate was applied to HBD-2 detection on the surface of contaminated chicken skin. This study provides a simple and highly sensitive method for the detection of HBD-2, offering a novel approach for the non-invasive rapid diagnosis of psoriasis and the monitoring of disease progression. Graphical
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Huiqiao Liu,Jiakun Zhang,Fan Zhang,Jiale Yang,Yuyuan Lu,Jun-Tao Cao,Kangzhe Cao
Accurate detection of theophylline (THP) is often hindered by interference from structurally similar compounds and limitations in sensitivity. To address these challenges, this work introduces a novel voltage-assisted molecularly imprinted surface-enhanced Raman scattering (MIP-SERS) platform by integrating in-situ electrokinetic preconcentration with specific molecular recognition. The key innovation lies in applying an optimized potential during incubation to actively drive and accelerate THP binding kinetics at imprinted sites, thereby overcoming the intrinsic diffusion limitations of passive MIP sensors and improving the SERS intensity. As a result, the sensor achieves an exceptional detection limit of 0.52 nM, and a linear range of 1.0 × 10 −9 -1.0 × 10 −5 M, while demonstrating robust quantification in complex tea matrices. This platform has significant potential for precise therapeutic drug monitoring and food safety control, establishing a new paradigm for sensitive detection in complex samples.
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高影响因子
LANGMUIR
·
2024
·
IF 3.7
Tianran Wang,Chushu Zhu,Peitao Dong
Surface-enhanced Raman scattering (SERS) is a novel spectroscopic technique that enables the identification of analytes through analysis of their unique chemical signatures. Its high sensitivity, specificity, and rapid response make it a valuable tool in a range of fields, including biological detection, food safety, and environmental monitoring. However, traditional SERS nanoparticle substrates are susceptible to instability and agglomeration. In this study, Ta4C3 MXene/gold nanostar (AuNSs) hybrids were prepared as SERS substrates. By optimizing the experimental conditions of AuNSs, the optimal preparation method for the Ta4C3 MXene/AuNSs composite structure was identified. The addition of MXene to the Ta4C3 MXene/AuNSs hybrid substrates was found to enhance the sensitivity of the composite for Raman detection, as evaluated using 4-aminothiophenol (PATP) as a Raman molecule. This improvement in sensitivity is attributed to the enhanced electromagnetic properties of the hybrid substrates, which facilitate more efficient charge transfer and enhance the Raman scattering process. The limit of detection (LOD) of Ta4C3 MXene/AuNSs is 10–9 M for PATP and 10–7 M for thiram solutions. In addition, good reproducibility and spatial uniformity were obtained for the Raman signals of Ta4C3 MXene/AuNSs. Furthermore, Ta4C3 MXene/AuNSs were combined with filter paper to create paper-based SERS substrates that could be used for detection. By directly wiping the apple peel and subsequently detecting the thiram residues using Ta4C3 MXene/AuNSs, the level of detection of thiram residues on the peel surface was down to 7.8 ng/cm2.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-11
·
IF 5.3
Gu Yingqiu,Fang Puhao,Chen Yu,Xie Tianhua,Yang Guohai,Qu Lulu
A miniature multi-channel surface-enhanced Raman scattering (SERS) sensor based on polydimethylsiloxane (PDMS) is constructed to achieve rapid delivery of polluted water and specific identification of multiple components. Hg 2+ , organic pollutants, and sodium nitrite are successfully identified by the multi-channel SERS sensor using Cy5, cyclodextrin, and urea in the corresponding detection area. This multi-channel sensor exhibits excellent sensitivity and specificity, with detection limits of 3.2 × 10 −10 M for Hg 2+ , 1.0 × 10 −8 M for aniline, 6.9 × 10 −9 M for diphenylamine, 9.1 × 10 −8 M for PCB-77, and 7.5 × 10 −9 M for pyrene, and 5.0 × 10 −7 M for sodium nitrite. Compared with traditional analysis techniques, this method exhibited excellent recovery for the water pollutants ranging from 82.1 to 115.8%. The PDMS-based microchannel allows for simultaneous and rapid identification of multiple environmental pollutants, offering a portable detection method for emergency testing of environmental pollutants and routine determination of water pollutants. Graphical Abstract
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高影响因子
Crystals
·
2022
·Vol. 12
·期 7
·884
·
IF 2.67
Yuanzhao Wu,Mingjie Chen,Haohao Bai,Binjie Wang,Jiye Wang,Yazhou Qin,Weixuan Yao
Hollow urchin-like Au nanocrystals have been widely studied due to their excellent surface plasmon resonance properties and large specific surface area, but the controllable preparation of hollow urchin-like Au nanocrystals is still a challenge. In this article, we successfully prepared hollow urchin-like Au nanocrystals using HAuCl4·3H2O and AgNO3as precursors and ascorbic acid as the reducing agent. No surface ligands or polymer stabilizers are required in the preparation process. HAuCl4·3H2O and AgNO3will first form AgCl cubes, then the reducing agent, ascorbic acid, will reduce the Au3+in the solution to Au0, and Au0will be deposited on the pre-formed AgCl cubes to form AgCl@Au nanocrystals. We characterized the morphology of the prepared Au nanocrystals by scanning electron microscopy and found that by increasing the amount of HAuCl4·3H2O in the reaction, the surface morphology of the Au nanocrystals would change from a rough spherical shape to an urchin-like shape. By further increasing the amount of the precursor HAuCl4·3H2O, urchin-like Au will convert into flake-like morphology. The AgCl in the interior was removed with ammonia water, and finally, hollow urchin-like Au crystals were formed. In addition, we used R6G molecule to explore the surface-enhanced Raman spectroscopy (SERS) enhancement effect of prepared Au crystals. The results show that the minimum detectable concentration of R6G reaches 10−8M. Moreover, we applied hollow urchin-like Au nanocrystals as catalysts and SERS enhancing materials to detect the photocatalytic reaction of 4-NTP. We used a 785 nm laser as both the SERS light source and the catalytic light source to monitor the photocatalytic effect of the laser on 4-NTP in situ by adjusting the laser power.Keywords:Au nanocrystals;sea urchin-like structure;SERS;photocatalytic reaction
研究领域:
材料科学
·
化学
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高影响因子
Scientific Reports
·
2015
·Vol. 5
·期 1
·1-10
·
IF 5.578
Zhang Youlin,Li Xiaokun,Xue Bin,Kong Xianggui,Liu Xiaomin,Tu Langping,Chang Yulei
Silica-coated SERS tags have been attracting greater attention in recent years. However, the reported methods to synthesize these tags are tedious and often subjected to the limited signal intensity. Here, we report a facile and general method to prepare the silica-coated Ag SERS tags with the enhanced signal intensity by no introducing the primers. This approach mainly depends on the colloidal stability of the Ag NPs in alcohol solution. By decreasing the concentration of salt in Ag NP solution, the citrate-stabilized Ag NPs can be well dispersed in alcohol solution. Based on this, the Ag SERS tags can be directly coated with thickness-controlled and homogeneous silica shells. This approach is highly reproducible for silica shell growth and signal intensity, not depending on the properties of Raman molecules, proved by 7 kinds of the Raman molecules. Moreover, this kind of SERS tags coated with silica hold the stronger SERS signals than the traditional method due to no interference from the priming molecules.
研究领域:
材料科学
·
分析化学
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高影响因子
Materials Research Express
·
2020
·Vol. 7
·期 9
·095012
·
IF 1.929
Menghui Wan,Haodong Zhao,Zhihua Wang,Yanbao Zhao,Lei Sun
In this paper, a convenient method to fabricate flexible and free-standing surface-enhanced Raman scatting (SERS) substrates for direct bacteria detection without aptamer bonding is presented. SiO2 nanofibers were prepared via electrospinning and calcination by using tetraethyl orthosilicate as the precursor. Subsequently, it was coated with polydopamine (PDA) by self-polymerization. Finally, Ag@PDA@SiO2 nanofibrous membranes were obtained through in situ growth of Ag nanoparticles in Tollens' reagents. The as-prepared Ag@PDA@SiO2 composite nanofibrous membranes were characterized by techniques of scanning electron microscopy, transmission electron microscopy, X-ray powder diffraction, energy-dispersive x-ray spectroscopy and thermo gravimetric analysis. The flexibility of the as-prepared nanofibrous membranes were verified simply through manual folding. Small molecule probes of 4-mercaptophenol (4-MPh) and 4-mercaptobenzoic acid (4-MBA) were chosen to investigate the SERS sensitivity of the as-prepared Ag@PDA@SiO2 as free-standing substrates. Furthermore, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), as typical strains of Gram-negative and Gram-positive bacteria, were performed to directly SERS detection by dropping bacteria suspension onto the fibrous membranes without any previous treatment, such as aptamer combination. In addition, the antimicrobial properties of the as-prepared Ag@PDA@SiO2 electrospinning nanofibrous membranes were tested by inhibition zone and turbidity methods. The results show that Ag nanoparticles with an average diameter of 50 nm are uniformly deposited on the surface of electrospinning nanofibers, and the as-prepared Ag@PDA@SiO2 nanofibrous membranes are flexible. As SERS substrates, it shows a rather high detection limitation of 10−11 mol l−1 for 4-MPh and 4-MBA. More importantly, this substrate can be applied for bacteria label-free SERS detection, i.e., complicated procedures are avoided. Meanwihile, the as-prepared Ag@PDA@SiO2 nanofibrous membranes exhibit excellent antibacterial properties. Thus, it has application prospects in trace bacteria detection and water purification.
研究领域:
材料科学
·
生物医学工程
·
分析化学
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Yiran Tian,Xiuting Li,Fuyan Wang,Chenjie Gu,Ziqi Zhao,Hongjie Si,Tao Jiang
Here, straight upward Au nanowires (NWs) were successfully grown onto Fe 3 O 4 @TiO 2 matrix through a seed-mediated strategy to intensively improve its photocatalysis and SERS performances, facilitating a peculiar recyclable surface-enhanced Raman spectroscopy (SERS)-based immunoassay of CA19–9 in liquid form based on visible light irradiation. Such immunoassay was also supported by a smart heterobifunctional cross-linking agent-mediated probe of anti-CA19–9/4-MBA without metal nanoparticles . A low limit of detection of 5.65 × 10 −4 IU mL −1 and a wide linear range from 1000 to 0.001 IU mL −1 were demonstrated through repeated constructing the sandwich immunostructure with only one batch of nanocomposites . Moreover, the actual levels of CA19–9 for colorectal cancer patients were readily measured by the recyclable immunoassay, the results of which are principally consistent with the conventional CLIA detection. Thus, such a green strategy of visible light-induced recyclable immunoassay could be expected to have a potential practicability in the clinical diagnoses of cancer.
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NANOTECHNOLOGY
·
2022
·Vol. 33
·期 13
·135501
·
IF 3.953
Jun Dong,Yan Wang,Qianying Wang,Yi Cao,Qingyan Han,Wei Gao,Yongkai Wang,Jianxia Qi,Mengtao Sun
Surface-enhanced Raman scattering (SERS) is recognized as one of the most favored techniques for enhancing Raman signals. The morphology of the SERS substrate profoundly affects molecular Raman spectra. This study aimed to construct a ring-mounted nanostructured substrate via liquid–liquid two-phase self-assembly incorporated with anodic aluminum oxide (AAO) membrane transfer techniques. High-density nanoparticles (NPs) assembled on AAO membranes were ascribed to reduce the diameters of the nanopores, with Au–Ag alloy NPs to regulate the dielectric constant so as to reveal the local surface plasmon resonance tunability. SERS engineered in this way allowed for the fabrication of a ring-mounted nanostructured substrate where the distribution density of NPs and dielectric constant could be independently fine-tuned. High SERS activity of the substrate was revealed by detecting the enhanced factor of crystal violet and rhodamine 6G molecules, which was up to 1.56 × 106. Moreover, SERS of thiram target molecules confirmed the supersensitivity and repeatability of the substrate as a practical application. The results of this study manifested a low-cost but high-efficiency ring-mounted nanostructured SERS substrate that might be suitable in many fields, including biosensing, medical research, environmental monitoring, and optoelectronics.
研究领域:
材料科学
·
光学
·
生物传感
·
环境监测
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高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-10
·
IF 5.7
Wang Zedong,Zhang Liang,Sun Lian,Bao Siqi,Liu Dajun,Li Hongji,Liu Yuming
Silver nanocubes monolayer-modified polydimethylsiloxane (Ag NC/PDMS) flexible SERS substrates have been prepared by a three-phase interface self-assembly procedure. The combination of this method with membrane technology brings nanoparticles in close proximity, densely, and regularly arranged in monolayers over a large area, leading to excellent SERS properties. Considering the complexity of practical detection, molecular imprinted polymers (MIPs) were anchored on the surface of SERS substrate and applied to selective detection of microcystin-LR (MC-LR). It is worth mentioning that the SERS imprinted membranes (AP-MIMs) were still clearly detected at a concentration of 0.1 µg·L −1 of MC-LR in drinking water, and the detection limit was as low as 0.0067 µg·L −1 . The substrate exhibited excellent uniformity with a relative standard deviation (RSD) of 6.1%. In the presence of interference molecules, AP-MIMs exhibited excellent selectivity for MC-LR. Furthermore, in the spiking and recovery tests of practical lake water samples, the method showed excellent recoveries ranging from 96.47 to 105.31%. It has been demonstrated that the prepared AP-MIMs can be applied to sensitive and specific detection of trace amounts of MC-LR in drinking water. Graphical
研究领域:
分析化学
·
环境科学
·
材料科学
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