Journal of Materials Chemistry B
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2017
·Vol. 5
·期 34
·7153-7163
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IF 4.543
Yan Li,Zuyuan Luo,Xiao Xu,Yongliang Li,Siqi Zhang,Ping Zhou,Yi Sui,Minjie Wu,En Luo,Shicheng Wei
Several bone diseases, including arthritis, fracture and osteoporosis, have a pathophysiologically important inflammatory component. Sustained inflammation can result in delayed bone healing. Therefore, to promote bone repair, it is important to inhibit inflammatory bone erosion and suppress pro-inflammatory mediators. In this study, aspirin significantly enhanced immunomodulation and osteogenic differentiation in human mesenchymal stem cells (hMSCs). Additionally, an osteogenic BFP-1 peptide-decorated substrate (PS-PEP) enhanced osteogenic differentiation of aspirin-treated hMSCs compared to a pristine substrate. Alkaline phosphatase assay, quantitative real-time polymerase chain reaction, immunostaining and Alizarin Red S staining revealed that aspirin-treated hMSCs cultured on PS-PEP exhibited enhanced osteogenesis compared with untreated cells. Thus, we report here that the anti-inflammatory and osteogenic effects of aspirin promote the activity and osteogenesis of hMSCs. The combination of aspirin and an osteogenic BFP-1 peptide-decorated substrate suppresses the production of pro-inflammatory mediators and promotes osteogenic differentiation of hMSCs; therefore, this novel strategy has potential for application in cell therapy and bone tissue engineering.
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RSC Advances
·
2016
·Vol. 6
·期 24
·20202-20210
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IF 3.289
Tianlin Gao,Weiwei Cui,Zongliang Wang,Yu Wang,Ya Liu,Ponnurengam Sivakumar Malliappan,Yoshihiro Ito,Peibiao Zhang
Lack of bioactivity has seriously restricted the development of biodegradable implants in bone tissue engineering. In this study, Bone Morphogenic Protein 2 (BMP-2) was photo-immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA composites via photo-reactive gelatin (Az-Gel). The results demonstrate that the Az-Gel facilitates efficient and long-term immobilization of peptides on the surface of PLGA. The immobilization of Az-Gel enhanced the adhesion and proliferation of Mouse Adipose-Derived Stem Cells (ADSCs), while HA incorporation facilitated cellular osteodifferentiation. The immobilization of BMP-2 induced the osteogenesis of the ADSCs, indicated by alkaline phosphatase activity, quantitative real-time polymerase chain reaction (qRT-PCR) analysis and mineralization on the deposited substrates. Furthermore, the surface immobilization of BMP-2 could not only reduce the amount of growth factor, but also have a long-term osteoinductive effect. Therefore, it is considered that the surface modification method has a great potential for the enhancement of osteointegration of biodegradable bone implants.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2016
·
IF 3.782
Hong Liu,Yan Tan,Lisi Xie,Lei Yang,Jing Zhao,Jingxuan Bai,Ping Huang,Wugen Zhan,Qian Wan,Chao Zou,Yali Han,Zhiyong Wang
Stem cells hold great promise for treating various diseases. However, one of the main drawbacks of stem cell therapy is the lack of non-invasive image-tracking technologies. Although magnetic resonance imaging (MRI) and near-infrared fluorescence (NIRF) imaging have been employed to analyse cellular and subcellular events via the assistance of contrast agents, the sensitivity and temporal resolution of MRI and the spatial resolution of NIRF are still shortcomings. In this study, superparamagnetic iron oxide nanocrystals and IR-780 dyes were co-encapsulated in stearic acid-modified polyethylenimine to form a dual-modality contrast agent with nano-size and positive charge. These resulting agents efficiently labelled stem cells and did not influence the cellular viability and differentiation. Moreover, the labelled cells showed the advantages of dual-modality imaging in vivo.
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BIOLOGICAL & PHARMACEUTICAL BULLETIN
·
2017
·
IF 1.683
Yan Fang,Lisheng Chu,Lin Li,Jun Wang,Yan Yang,Jingjing Gu,Jianping Zhang
Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation is one of the new therapeutic strategies for treating ischemic stroke. However, the poor survival rate of transplanted BMSCs in ischemic tissue limits the therapeutic efficacy of this approach. Oxidative stress is a major mechanism underlying the pathogenesis of brain ischemia and has a negative impact on the survival of transplanted BMSCs. Tetramethylpyrazine (TMP) has been reported to possess potent antioxidant activity. In the present study, we aimed to investigate the protective effects of TMP pretreatment on BMSCs survival of hydrogen peroxide (H2O2)-induced apoptosis in vitro and to elucidate the potential antiapoptotic mechanisms of TMP pretreatment on BMSCs. BMSCs were pretreated with TMP (10, 25, 50, 100, and 200 µmol/L) for 24 h and then exposed to 500 µmol/L of H2O2 for 24 h. We found that TMP pretreatment significantly increased cell viability and decreased cell apoptosis and intracellular reactive oxygen species (ROS) generation. Furthermore, the protective effects of TMP were related to increased Bcl-2 expression, attenuated Bax expression, and enhanced levels of phosphorylated Akt (p-Akt) and extracellular regulated protein kinases1/2 (p-ERK1/2). Further studies found that these beneficial effects of TMP were significantly blocked by wortmannin (an inhibitor of phosphoinositide-3 kinase (PI3K)) or PD98059 (an inhibitor of ERK1/2). In conclusion, our results confirm that TMP protects BMSCs against H2O2-induced apoptosis by regulating the PI3K/Akt and ERK1/2 signaling pathways, suggesting that TMP may be used in combination with BMSCs to improve cell survival for the treatment of ischemic stroke.
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RSC Advances
·
2015
·Vol. 5
·期 38
·30268-30274
·
IF 3.84
Xiaopeng Huang,Yuqin Li,Xiang Huang,Yaowen Chen,Wenhua Gao
An ingenious sensing strategy for detecting thrombin in human serum has been developed on the basis of a hairpin DNA sequence and resonance light scattering (RLS) technique. A thrombin aptamer sequence was embedded inside the hairpin DNA strand (H-eTBA), which was designed to be the loop-stem structure. Moreover, methylene blue (MB) was utilized as the RLS signal indicator according to its different affinity to single or double stranded DNA. Upon the addition of thrombin, the thrombin aptamer inside H-eTBA interacted specifically with thrombin. Thus the conformation of H-eTBA would change. After the introduction of the DNA strand (CTBA), which was complementary to H-eTBA, the amount of double stranded DNA would decrease as a consequence. Later when MB solution was added, the RLS signal would present various response values based on different amounts of thrombin. The determination of thrombin in human serum could be obtained with a detection limit of 0.32 nM and this specific sensor could be applied to detect thrombin practically. Furthermore, this aptasensor showed quite good selectivity and simplicity toward thrombin. Finally, the proposed sensing method showed its superiority with selectivity and practicability, which could be used as a simple platform for thrombin detection.
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CRYSTENGCOMM
·
2013
·Vol. 15
·期 41
·8330-8336
·
IF 3.879
Yamei Li,Shidong Ji,Yanfeng Gao,Hongjie Luo,Shaotang Li,Meng Jiang,Yijie Zhou,Rong Li,Bingbing Wang,Ping Jin
This paper presents a novel vapor–solid growth approach of stem-like 1D vanadium oxide nanostructures in a core-regenerated fashion on VO2@TiO2 core–shell rods under a very low temperature (400 °C). The composition and microstructure of the as-grown nanostructures were analyzed by HRTEM, EDS and XPS, confirming the vapor–solid mechanism. The confinement effect of the shell was analyzed by varying experimental parameters (coating thickness and heating rate), and the shell was found to play a critical role in generating and accumulating vapor with supersaturated concentration for the nanocrystal nucleation. Additionally, PVP was added as a surface modifier to favor the growth, probably by forming carbonaceous/nitrogen gaseous species as carrier media, as deduced by FTIR analysis. By exploring the impact of different heating rates, we found that the mass transfer process was facilitated only using a relatively high heating rate (>1.33 °C s−1). However, such a growth was still limited only within the early stages (~15 min), because the confined vacuum interface will disappear with the progress of the crystallization of the nanoporous anatase shell. A unique 1D rods to 2D nano-flakes evolution can be achieved by increasing the heating rate from 1.3 °C s−1 to over 8 °C s−1. These findings provide a valuable understanding on the thermodynamics and kinetics during an unique VS based nanocrystal growing process, and also showed a new method to obtain hierarchical heterogeneous nanostructures.
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INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
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2015
·
IF 4.046
Jie Ding,Zihua Tang,Jiarong Chen,Haosong Shi,Jianling Chen,Cuicui Wang,Cui Zhang,Liang Li,Ping Chen,Jinfu Wang
Sensorineural hearing loss and vestibular dysfunction have become the most common forms of sensory defects. Stem cell-based therapeutic strategies for curing hearing loss are being developed. Several attempts to develop hair cells by using chicken utricle stromal cells as feeder cells have resulted in phenotypic conversion of stem cells into inner ear hair-cell-like cells. Here, we induced the differentiation of human embryonic stem cells (hESCs) into otic epithelial progenitors (OEPs), and further induced the differentiation of OEPs into hair-cell-like cells using different substrates. Our results showed that OEPs cultured on the chicken utricle stromal cells with the induction medium could differentiate into hair-cell-like cells with stereociliary bundles. Co-culture with stromal cells, however, may be problematic for subsequent examination of the induced hair-cell-like cells. In order to avoid the interference from stromal cells, we cultured OEPs on laminin with different induction media and examined the effects of the induction medium on the differentiation potentials of OEPs into hair-cell-like cells. The results revealed that the culture of OEPs on laminin with the conditioned medium from chicken utricle stromal cells supplemented with EGF and all-trans retinoic acid (RA) could promote the organization of cells into epithelial clusters displaying hair-cell-like cells with stereociliary bundles. These cells also displayed the expected electrophysiological properties.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Danle Lin,Ziyu Zhou,Mengdan Zhang,Songyou Yao,Lingling Yuan,Meng Xu,Xiaoyue Zhang,Xiaoli Hu
Biophysical and biochemical cues of biomaterials can regulate cell behaviors. Dental pulp stem cells (DPSCs) in pulp tissues can differentiate to odontoblast-like cells and secrete reparative dentin to form a barrier to protect the underlying pulp tissues and enable complete pulp healing. Promotion of the odontogenic differentiation of DPSCs is essential for dentin regeneration. The effects of the surface potentials of biomaterials on the adhesion and odontogenic differentiation of DPSCs remain unclear. Here, poly(vinylidene fluoride-trifluoro ethylene) (P(VDF-TrFE)) films with different surface potentials were prepared by the spin-coating technique and the contact poling method. The cytoskeletal organization of DPSCs grown on P(VDF-TrFE) films was studied by immunofluorescence staining. Using atomic force microscopy (AFM), the lateral detachment forces of DPSCs from P(VDF-TrFE) films were quantified. The effects of electrical stimulation generated from P(VDF-TrFE) films on odontogenic differentiation of DPSCs were evaluated in vitro and in vivo. The unpolarized, positively polarized, and negatively polarized films had surface potentials of −52.9, +902.4, and −502.2 mV, respectively. DPSCs on both negatively and positively polarized P(VDF-TrFE) films had larger cell areas and length-to-width ratios than those on the unpolarized films (P < 0.05). During the detachment of DPSCs from P(VDF-TrFE) films, the average magnitudes of the maximum detachment forces were 29.4, 72.1, and 53.9 nN for unpolarized, positively polarized, and negatively polarized groups, respectively (P < 0.05). The polarized films enhanced the mineralization activities and increased the expression levels of the odontogenic-related proteins of DPSCs compared to the unpolarized films (P < 0.05). The extracellular signal-regulated kinase (ERK) signaling pathway was involved in the odontogenic differentiation of DPSCs as induced by surface charge. In vivo, the polarized P(VDF-TrFE) films enhanced adhesion of DPSCs and promoted the odontogenic differentiation of DPSCs by electrical stimulation, demonstrating a potential application of electroactive biomaterials for reparative dentin formation in direct pulp capping.
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JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Wenbo Yang,Qing Pan,Yizhong Peng,Yuxiang Hu,Yongzhi Cui,Zengwu Shao,Chunqing Meng,Hong Wang,Wei Huang
Steroid-induced osteonecrosis of the femoral head (SANFH) is a common hip joint disease that imposes a heavy economic burden on society. Patients continue to experience bone necrosis even after discontinuing glucocorticoid therapy, and the specific mechanisms require further investigation. The results of this study demonstrate that exosomes secreted by damaged vascular endothelial cells in SANFH lesions may be a crucial factor leading to abnormal adipogenic differentiation of bone marrow stromal cells (BMSCs). We also found that adipogenically differentiated BMSCs can secrete increased pro-inflammatory factors, which contribute to the maintenance of endothelial cell damage, thereby creating a vicious cycle. Due to endothelial damage and abnormal differentiation of BMSCs, insufficient bone repair occurs, leading to spontaneous disease progression. Based on our newly explored mechanism, we designed a nanomaterial, M-SiO 2 @Icariin@EBHM, that simultaneously targets the repair of damaged vascular endothelium and re-regulation of BMSCs, and we assessed its role in delaying SANFH progression. We found that the M-SiO 2 @Icariin@EBHM nanomaterial can target SANFH lesions when applied systemically, effectively clearing the elevated reactive oxygen species in vascular endothelial cells caused by glucocorticoids and regulating the osteogenic differentiation of BMSCs. This process breaks the vicious cycle while promoting bone repair and significantly inhibiting SANFH progression, revealing its potential as a promising treatment strategy for SANFH.
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ACS Nano
·
2024
·
IF 15.8
Meng Sun,Min Li,Min Hu,Yueyun Fan,Yanhong Liu,Jian Sun,Jinfeng Zhang
Acute liver failure (ALF) is a serious global disease characterized by rapid onset and high mortality. Currently, the clinical treatment of ALF faces considerable hurdles due to limited medication options and the scarcity of liver transplants. Despite biomacromolecules such as hepatocyte growth factor (HGF) and glutathione (GSH) having been applied for ALF symptom relief in the clinic, they still face substantial challenges including poor stability, difficulty in acting on intracellular targets, and inadequate therapeutic outcome. In this work, by taking advantage of the innate targeting and regenerative capabilities of mesenchymal stem cells (MSCs), we harnessed MSC-derived exosomes as natural bioactive carriers for the simultaneous delivery of HGF and GSH, forming a fully bioactive nanodrug termed HG@Exo. Impressively, the HG@Exo demonstrated potent therapeutic effects against both carbon tetrachloride (CCl4)- and extreme hepatectomy-induced ALF through multiple mechanisms, including regulation of oxidative stress, reduction of inflammation, and promotion of hepatocyte regeneration, which were facilitated by its inflammation-targeting to damaged liver tissues. Furthermore, an FDA-approved near-infrared fluorescent dye, indocyanine green (ICG), has been incorporated into the exosomes (HGI@Exo) to endow them with real-time in vivo tracking capability, which showed favorable liver accumulation of the HGI@Exo in both CCl4- and surgery-induced ALF animal models, providing crucial insights into their biodistribution and therapeutic efficacy. Overall, the presented fully bioactive nanodrugs with targeting and theranostic abilities hold significant promise for potentiating the therapeutic efficacy of biomacromolecules for the improved treatment of ALF and other inflammatory diseases.
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LIFE SCIENCES
·
2024
·
IF 5.2
Tuoying Jiang,Jianan Huang,Bo Xu,Zhen Ge,Yi Li,Leiting Wei,Luyang Yu,Jinying Li
Aims Parkinson's disease (PD) remains a substantial clinical challenge due to the progressive loss of midbrain dopaminergic (DA) neurons in nigrostriatal pathway. In this study, human amniotic epithelial stem cells (hAESCs)-derived dopaminergic neuron-like cells (hAESCs-DNLCs) were generated, with the aim of providing new therapeutic approach to PD. Materials and methods hAESCs, which were isolated from discarded placentas, were induced to differentiate into hAESCs-DNLCs by following a “two stages” induction protocol. The differentiation efficiency was assessed by quantitative real-time PCR (qRT-PCR), immunocytochemistry (ICC), and ELISA. Immunogenicity, cell viability and tumorigenicity of hAESCs-DNLC were analyzed before in vivo experiments. Subsequently, hAESCs-DNLCs were transplanted into PD rats, behavioral tests were monitored after graft, and the regeneration of DA neurons was detected by immunohistochemistry (IHC). Furthermore, to trace hAESCs-DNLCs in vivo , cells were pre-labeled with PKH67 green fluorescence. Key findings hAESCs were positive for pluripotent markers and highly expressed neural stem cells (NSCs) markers. Based on this, we established an induction method reliably generates hAESCs-DNLCs, which was evidenced by epithelium-to-neuron morphological changes, elevated expressions of neuronal and DA neuronal markers, and increased secretion of dopamine. Moreover, hAESCs-DNLCs maintained high cell viability, no tumorigenicity and low immunogenicity, suggesting hAESCs-DNLCs an attractive implant for PD therapy. Transplantation of hAESCs-DNLCs into PD rats significantly ameliorated motor disorders, as well as enhanced the reinnervation of TH + DA neurons in nigrostriatal pathway. Significance Our study has demonstrated evident therapeutic effects of hAESCs-DNLCs, and provides a safe and promising solution for PD.
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Sustainability
·
2024
·Vol. 16
·期 7
·2629
·
IF 3.3
Haifeng Wang,Yang Yang,Mengping Wang,Runjiao Yuan,Wenyi Song,Lin Wang,Ni Liang,Jiayi Shi,Jing Li
In the present study, banana pseudo-stem (BS) was pyrolyzed under anaerobic conditions without any physical or chemical modification. Their properties, as well as their sorption affinity to ofloxacin (OFL), were studied. As a result, oxalates and KCl formed at a relatively low temperature of 300 °C, while bicarbonates generally formed at a pyrolysis temperature above 400 °C. Surface functional groups of BS biochars facilitated OFL sorption mainly via specific interactions including electronic attraction (EA), π–π electron donor–acceptor (π–π EDA) interaction, the ordinary hydrogen bond (OHB), and the negative charge-assisted hydrogen bond ((−)CAHB). Except for (−)CAHB, these interactions all decreased with an elevated pH, resulting in overall decreased OFL sorption. Significant OFL sorption by BS biochars produced at 300 °C, observed even at an alkaline condition was attributed to (−)CAHB. Micropores formed in BS biochar prepared at 500 °C, with a specific surface area as high as 390 m2g−1after water washing treatment. However, most micropores could not be accessed by OFL molecules due to the size exclusion effect. Additionally, the inherent K-containing salts may hinder OFL sorption by covering the sorption sites or blocking the inner pores of biochars, as well as releasing OH−into the solution. Thus, BS biochar produced at 300 °C is an excellent sorbent for OFL removal due to its high sorption ability and low energy. Our findings indicate that biochar techniques have potential win–win effects in recycling banana waste with low energy and costs, and simultaneously converting them into promising sorbents for the removal of environmental contaminants.
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危险品化学品经营许可证（带存储）