Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Zhang Haipeng,Zhao Xiaoou,Wang Xiaoyu,Wang Xinyue,Gu Jinyang,Liu Zhongliang,Kong Lingcong,Chen Jingrui,Ma Hongxia
The widespread use of commercial antibiotics has led to the emergence of multidrug resistance in pathogenic bacteria, posing a significant threat to human health and underscoring the urgent need for alternative antimicrobial agents. In this study, a bacteriocin-producing strain, Bacillus velezensis FS-3 ( B. velezensis FS-3), was isolated from soil in Changbaishan, China. Fermentation conditions were optimized to enhance both bacteriocin activity and yield. A novel bacteriocin, PFS-3, was purified from B. velezensis FS-3 using hydrochloric acid precipitation, organic solvent extraction, and preparative reversed-phase high-performance liquid chromatography. Liquid chromatography-mass spectrometry/mass spectrometry analysis determined its molecular weight to be 929.16 Da, and amino acid sequencing of this peptide revealed eight amino acids (STYLFEGL). To date, the biological properties of PFS-3 have not been reported. We demonstrate here that PFS-3 exhibits low toxicity and remarkable stability under diverse conditions, including variations in temperature, pH, and the presence of metal ions or organic reagents. PFS-3 displayed broad-spectrum antimicrobial activity, with particularly strong activity against Gram-negative bacteria. Notably, its minimum inhibitory concentration against multidrug-resistant Escherichia coli B2 (MDR E. coli B2) was 16 μg/mL. Mechanistic investigations revealed that bacteriocin PFS-3 exerts bactericidal effects on MDR E. coli B2 by disrupting cardiolipin in the outer membrane of the cells. Furthermore, in vivo experiments demonstrated that PFS-3 significantly improved survival rates in infection models of MDR E. coli . In conclusion, PFS-3 is a newly identified bacteriocin with strong antibacterial activity, high stability, safety, and a favorable therapeutic index, highlighting its potential applications in the food industry and biopharmaceuticals for combating MDR E. coli infections.
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高影响因子
JOURNAL OF BIOSCIENCES
·
2020
·Vol. 45
·期 1
·1-7
·
IF 1.645
Jin Jia-Yuan,Chen Ye,Wang Xing-You,Li Chen-Ming,Chen Wei-Lin,Li Li
Herein, we found that serum concentration of superoxide dismutase 3 (SOD3) was significantly reduced in children with mycoplasma pneumonia (MP) infection. To study the roles of SOD3 in inflammatory regulation of MP infection, human A549 type II alveolar epithelial cells were stimulated with 10 7 CCU/ml of MP to build MP infection in vitro . Secretion of pro-inflammatory cytokine interleukin (IL)-8 and tumor necrosis factor (TNF)-α were measured via enzyme-linked immunosorbent assay (ELISA) to assess the inflammatory response of A549 cells. Levofloxacin (LVFX) was used as an anti-inflammatory drug while recombinant TNF-α was used as an inflammatory promotor in MP-infected cells. Transcriptional activity of nuclear factor (NF)-кB was assessed by detecting protein levels of nuclear NF-кB and cytoplasm NF-кB using Western blot analysis. Our data suggested that the expression of SOD3 mRNA and protein, as well as content of SOD3 in cultured supernatant, were time-dependently inhibited in MP-infected A549 cells. However, lentiviruses-mediated SOD3 overexpression alleviated inflammatory response of MP-infected A549 cells, and prevented the unclear translocation of NF-кB, as evidenced by obviously reducing the production of IL-8 and TNF-α in cell cultured supernatant, as well as decreasing nuclear NF-кB while increasing cytoplasm NF-кB.
Inspiringly, SOD3 overexpression induced anti-inflammatory effect and the inactivation of NF-кB was similar to that of 2 μg/ml of LVFX, but reversed by additional TNF-α treatment. Therefore, we can conclude that transcriptional activity of NF-κB was the underlying mechanism, by which SOD3 regulated inflammatory response in MP infection in vitro .
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Microbiome
·
2023
·Vol. 11
·期 1
·1-23
·
IF 15.5
Hu Jun,Chen Jianwei,Xu Xiaojian,Hou Qiliang,Ren Jing,Yan Xianghua
Background The intestinal epithelial barrier confers protection against the intestinal invasion by pathogens and exposure to food antigens and toxins. Growing studies have linked the gut microbiota to the intestinal epithelial barrier function. The mining of the gut microbes that facilitate the function of intestinal epithelial barrier is urgently needed.Results Here, we studied a landscape of the gut microbiome of seven pig breeds using metagenomics and 16S rDNA gene amplicon sequencing. The results indicated an obvious difference in the gut microbiome between Congjiang miniature (CM) pigs (a native Chinese breed) and commercial Duroc × [Landrace × Yorkshire] (DLY) pigs. CM finishing pigs had stronger intestinal epithelial barrier function than the DLY finishing pigs. Fecal microbiota transplantation from CM and DLY finishing pigs to germ-free (GF) mice transferred the intestinal epithelial barrier characteristics. By comparing the gut microbiome of the recipient GF mice, we identified and validated Bacteroides fragilis as a microbial species that contributes to the intestinal epithelial barrier. B. fragilis-derived 3-phenylpropionic acid metabolite had an important function on the enhancement of intestinal epithelial barrier. Furthermore, 3-phenylpropionic acid facilitated the intestinal epithelial barrier by activating aryl hydrocarbon receptor (AhR) signaling.Conclusion sThese findings suggest that manipulation of B. fragilis and 3-phenylpropionic acid is a promising strategy for improving intestinal epithelial barrier.
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Cell Chemical Biology
·
2020
·
IF 7.739
Giorgia Croppi,Yueyang Zhou,Rong Yang,Yunfei Bian,Mingtao Zhao,Youtian Hu,Benfang Helen Ruan,Jing Yu,Fang Wu
Summary H 2 S-producing enzymes in bacteria have been shown to be closely engaged in the process of microbial survival and antibiotic resistance. However, no inhibitors have been discovered for these enzymes, e.g., 3-mercaptopyruvate sulfurtransferase (MST). In the present study, we identified several classes of inhibitors for Escherichia coli MST (eMST) through high-throughput screening of ∼26,000 compounds. The thiazolidinedione-type inhibitors were found to selectively bind to Arg178 and Ser239 residues of eMST but hardly affected human MST. Moreover, the pioglitazone of this class concentration dependently accumulates the 3-mercaptopyruvate substrate and suppresses the H 2 S and reactive sulfane sulfur products in bacteria. Importantly, pioglitazone could potentiate the level of reactive oxygen species in cellulo and consequently enhance the antimicrobial effects of gentamicin and macrophages in culture. This study has identified the bioactive inhibitor of eMST, paving the way for the pharmacological targeting of eMST to synergistically control the survival of E. coli .
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2017
·Vol. 134
·期 46
·45484
·
IF 1.86
Teng Xue,Erjun Tang,Jian Zhou,Ruitao Han,Shaojie Liu
A coordinating ionic liquid (IL), 1-[(diethyl amine)amine]ethyl-3-methyl imidazolium chloride ([N 3 MIM]Cl), was prepared as an alternative to a simple organic ligand to coordinate to copper(I) bromide (CuBr). We, thereby, obtained a novel catalyst for atom transfer radical polymerization (ATRP) reactions. This catalyst was applied to the ATRP of methyl methacrylate in the IL 1-allyl-3-methyl imidazolium chloride ([AMIM]Cl). The chemical structures of the ILs obtained were confirmed by Fourier transform infrared spectroscopy, mass spectrometry, and 1 H-NMR analyses. The coordination ability of [N 3 MIM]Cl was assessed by cyclic voltammetry, and the redox potential of [N 3 MIM]Cl–CuBr was −0.507 V. The [N 3 MIM]Cl–CuBr complex was expected to be a markedly more active catalyst than the amine DETA–CuBr complex. The coordination mode toward CuBr was also examined. The [N 3 MIM]Cl–CuBr catalyst system showed good controllability in the aforementioned ATRP reaction in [AMIM]Cl. The Cu catalyst was easily separated from the obtained polymer with the coordinating IL as a ligand. Consequently, the coordinating IL overcame the shortcomings of traditional organic ligands, such as poor compatibility with IL media and poor separation of the catalyst from the polymer; this makes it highly promising for applications in the ATRP field. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45484.
研究领域:
高分子化学
·
催化化学
·
功能材料
·
绿色溶剂
·
配位化学
·
电化学
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REPRODUCTION FERTILITY AND DEVELOPMENT
·
2021
·Vol. 33
·期 12
·700-712
·
IF 2.311
Xin Zhan,Yiran Xie,Liping Sun,Qi Si,Hongkai Shang,Xin Zhan,Yiran Xie,Liping Sun,Qi Si,Hongkai Shang
This study explored the molecular mechanism underlying the effects of dexamethasone (DEX, 1 µM) on glucose transporters (GLUT) in JEG-3 human placental choriocarcinoma cells. JEG-3 cells were treated with DEX, an expression plasmid encoding human glucocorticoid receptor α (GRα), pcDNA3.1-GRα, GRα short interference (si) RNA, LY294002, xanthine oxidase (XO)/hypoxanthine (HX), rapamycin, insulin-like growth factor (IGF) 1, N-acetylcysteine (NAC) or phosphatidic acid (PA), and cell proliferation, apoptosis, mitochondrial membrane potential (MMP), human chorionic gonadotrophin (hCG) content, human placental lactogen (hPL) content, glucose uptake, reactive oxygen species levels and signalling pathway modulation were evaluated. Treatment of JEG-3 cells with DEX (1 µM), GRα siRNA, LY294002 (50 µM), XO/HX (7.2 µM/36 nM) or rapamycin (80 nM) inhibited cell proliferation, induced apoptosis, significantly decreased MMP and hCG and hPL content and increased ROS levels. In addition, glucose uptake was decreased through downregulation of the mRNA and protein expression of GRα, GLUT1 and GLUT3. Treatment of JEG-3 cells with GRα siRNA, LY294002, XO/HX or rapamycin inhibited phosphorylation of phosphatidylinositol 3-kinase (PI3K), Akt, glycogen synthase kinase 3 and mammalian target of rapamycin (mTOR) and induced the phosphorylation of AMP-activated protein kinase (AMPK) and tuberous sclerosis complex 2. The effects of GRα overexpression and IGF1 (100 nM), NAC (5 nM) or PA (100 µM) treatment on JEG-3 cells contrasted with those of DEX treatment. DEX blocked glucose uptake by downregulating GRα expression, which reduced GLUT1 and GLUT3 mRNA and protein expression, which, in turn, may have inhibited the PI3K/AKT/mTOR pathway and activated the ROS/AMPK pathway.
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ChemistrySelect
·
2025
·Vol. 10
·期 21
·e01335
·
IF 2
Yu-cheng Jiang,Bao-guang Zhu,Xiang-li Long
Graphical The production of 1-(4-chlorophenyl)-1H-pyrazol-3-ol from the oxidation of 1-(4-chlorophenyl)-pyrazolidin-3-one by air under the catalysis of Co(II)@AC has been enhanced by modifying the activated carbon with H₂C₂O₄ solution to increase the acidic groups and micropores on the carbon surface. The production of 1-(4-chlorophenyl)-1 H -pyrazol-3-ol by oxidizing 1-(4-chlorophenyl)- pyrazolidin-3-one with H 2 O 2 suffers from some shortcomings. The oxidation of 1-(4-chlorophenyl)-pyrazolidin-3-one to 1-(4-chlorophenyl)-1 H -pyrazol-3-ol can be realized by air catalyzed with Co(II)@AC (prepared by supporting cobalt(II) on activated carbon). Air is a good substitute for H 2 O 2 because it can minimize the cost, hazards, and pollution, which is beneficial to the improvement of the production process for pyraclostrobin. In this study, activated carbon has been modified by H 2 C 2 O 4 solution to improve the catalytic performance of Co(II)@AC. The experiments indicate that the best carbon is made by heating the carbon at 300 °C for 3 h with a heating rate of 5 °C/min in N 2 after being soaked in 0.4 mol/L H 2 C 2 O 4 solution at 50 °C for 12 h with an L/S ratio of 40/1 mL/g. The 1-(4-chlorophenyl)-1 H -pyrazol-3-ol concentration obtained by Co(II)@AC immobilized on the optimized carbon is 39.39% over that catalyzed by Co(II)@AC loaded on the original carbon. Characterization results reveal that modification with H 2 C 2 O 4 increases the specific surface area and enhances the acidity on the carbon surface. The oxidation rate of 1-(4-chlorophenyl)-pyrazolidin-3-one is primarily determined by the physical structure. The selectivity toward 1-(4-chlorophenyl)-1 H -pyrazol-3-ol is mainly influenced by the chemical characteristics.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2024
·Vol. 26
·期 30
·20273-20279
·
IF 2.9
Hao Huang,Chaohui Wei,Qiang Zhao,Aijun Zhou,Jingze Li
An in situ polymerization strategy is regarded as a promising approach to fabricate gel polymer electrolytes (GPEs) and improve interface contact between the electrolyte and electrodes, in which the initiator is initially dissolved in the precursor solution. Herein, aluminum trifluoromethanesulfonate (Al(OTf)3) is preloaded onto a separator sheet as the initiator to trigger the ring-opening reaction of 1,3-dioxolane (DOL). The polymer matrix near the separator has a higher crystallization degree than that far away from the separator. Fluoroethyl carbonate (FEC) is further introduced as a liquid plasticizer to produce an amorphous GPE for enhanced ionic conductivity and interfacial stability. As a result, the as-synthesized FEC based GPE exhibits a substantial ionic conductivity of 1.5 × 10−4 S cm−1 at room temperature, an expanded electrochemical window of 4.8 V, and a high Li+ transference number of 0.63. The symmetric Li|Li cell exhibits a stable lifespan for 650 h at 1 mA cm−2 and 1 mA h cm−2. Moreover, the LiFePO4 full cell exhibits stable cycling for 300 cycles at 1C with a capacity retention of 94.5%. This work provides a novel idea for the in situ synthesis of advanced GPEs toward practical application of solid-state lithium metal batteries.
研究领域:
材料科学
·
电化学
·
储能技术
·
高分子化学
·
固态电池
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Journal of Physical Chemistry C
·
2022
·
IF 4.177
Xuejia Luo,Mengyao Wang,Yuting Xie,Zhiqiang Wang,Qingshan Xie,Fanghui Lou,Guan Wang,Huming Hao,Liangxuan Yang,Guixin Wang
Severe deformation, thermal runaway, and low ionic migration do harm to the applications of silicon. Herein, a negative thermal expansion material of LiTi2(PO4)3 (LTP) with high ionic conductivity was synthesized and utilized to modify nanoscaled Si, and its effects were investigated. Si modified with 3 wt % LTP (SL3) shows the best performance, and its discharge capacities retain 849.4 and 410.9 mAh/g after 100 cycles at 2 A/g as temperatures are 25 and 60 °C, respectively, about 245.4 and 399.9% higher than those of Si. At 60 °C, the diffusion coefficient of lithium ions in Si is increased by 26 times, while the voltage difference between the main redox peaks, charge transfer resistance, and strain of the SL3 are 0.43 V, 171.4 Ω/cm2, and 211 με, respectively, around 15.7, 94.8, and 45.3 lower than those of the Si. The released heat of SL3 is declined to 175.4 J/g, about 85.9% that of the Si. The improvement mechanism of Si by LTP is discussed. It provides a path for enhancing the performance and safety of materials by decreasing heat and deformation hazards, improving the interface, and intensifying transfer.
研究领域:
材料科学
·
电化学
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Analytical Methods
·
2017
·Vol. 9
·期 30
·4439-4446
·
IF 1.9
Ping Zhang,Qian Yu,Yuhan He,Wentao Zhu,Zhiqiang Zhou,Lin He
The enantiomeric separation of fenpropathrin enantiomers on Lux Cellulose-1, Lux Cellulose-3 and Chiralpak IC chiral columns as well as enantioselective degradation of fenpropathrin in soil were investigated by reverse-phase high-performance liquid chromatography (RP-HPLC). The effects of the chiral stationary phase, mobile phase component, mobile phase ratio, temperature and thermodynamic parameters on resolution were carefully evaluated. Fenpropathrin enantiomers achieve a baseline separation (Rs = 2.30) on the Lux Cellulose-3 column with a mobile phase of methanol/water at a ratio of 85/15. Partial separations were achieved on Lux Cellulose-1 and Chiralpak IC columns with maximum Rs = 1.01 and Rs = 0.64, respectively. Enantioselective degradation of fenpropathrin enantiomers in soil was investigated based on the proposed separation method. The half-life (t1/2) of S-fenpropathrin and R-fenpropathrin was 17.8 days and 12.6 days, respectively, indicating that the degradation of fenpropathrin in soil was enantioselective, with R-fenpropathrin preferentially degraded. Furthermore, the formation and degradation of 3-PBA, the main metabolite of fenpropathrin, were also investigated in three soils. Soil microorganisms were confirmed as the main factor that is responsible for enantioselective degradation of fenpropathrin enantiomers in soil. Such results provide a new approach for fenpropathrin enantiomer separation and contribute to a comprehensive risk assessment of fenpropathrin at the enantiomer level.
研究领域:
环境科学
·
农药化学
·
微生物学
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2012
·Vol. 109
·期 9
·3492-3497
·
IF 9.681
Xufang Duan,Eric Block,Zhen Li,Timothy Connelly,Jian Zhang,Zhimin Huang,Xubo Su,Yi Pan,Lifang Wu,Qiuyi Chi,Siji Thomas,Shaozhong Zhang,Minghong Ma,Hiroaki Matsunami,Guo-Qiang Chen,Hanyi Zhuang
Odorant receptors (ORs) in olfactory sensory neurons (OSNs) mediate detection of volatile odorants. Divalent sulfur compounds, such as thiols and thioethers, are extremely potent odorants. We identify a mouse OR, MOR244-3, robustly responding to (methylthio)methanethiol (MeSCH2SH; MTMT) in heterologous cells. Found specifically in male mouse urine, strong-smelling MTMT [human threshold 100 parts per billion (ppb)] is a semiochemical that attracts female mice. Nonadjacent thiol and thioether groups in MTMT suggest involvement of a chelated metal complex in MOR244-3 activation. Metal ion involvement in thiol–OR interactions was previously proposed, but whether these ions change thiol-mediated OR activation remained unknown. We show that copper ion among all metal ions tested is required for robust activation of MOR244-3 toward ppb levels of MTMT, structurally related sulfur compounds, and other metal-coordinating odorants (e.g., strong-smelling trans-cyclooctene) among >125 compounds tested. Copper chelator (tetraethylenepentamine, TEPA) addition abolishes the response of MOR244-3 to MTMT. Histidine 105, located in the third transmembrane domain near the extracellular side, is proposed to serve as a copper-coordinating residue mediating interaction with the MTMT–copper complex. Electrophysiological recordings of the OSNs in the septal organ, abundantly expressing MOR244-3, revealed neurons responding to MTMT. Addition of copper ion and chelator TEPA respectively enhanced and reduced the response of some MTMT-responding neurons, demonstrating the physiological relevance of copper ion in olfaction. In a behavioral context, an olfactory discrimination assay showed that mice injected with TEPA failed to discriminate MTMT. This report establishes the role of metal ions in mammalian odor detection by ORs.
研究领域:
神经科学
·
分子生物学
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2018
·
IF 3.927
Zhen Li,Ling Yan,Jinjie Zhou,Xiaoli Wang,Yaqin Sun,Zhi-Long Xiu
The separation of 1,3-propanediol (1,3-PD), butyric acid (BA) and acetic acid (HAc) from the fermentation broth was studied by two-step salting-out extraction. In the first salting-out extraction, the partition coefficient and recovery of BA reached 42.21 and 96.42%, respectively, under the optimal condition of 25 wt% NaH 2 PO 4 /30 wt% n-butyl acetate. Subsequently, 91.28% of BA in the organic phase could be recovered through back-extraction when sodium carbonate solution was mixed with organic phase with initial phase ratio of n-butyl acetate solution to alkaline solution being 2:1, in which the molar ratio of sodium carbonate to organic acids was 3:5. Finally, 50% (v/v) ethanol (95%) was added to the bottom phase for the second step salting-out extraction. And the partition coefficient and recovery of 1,3-PD were 9.40 and 95.50%, and those of HAc were 7.46 and 94.40%, respectively. All the cells and most of the proteins (97.16%) could be removed. Effective separation of 1,3-PD from BA was realized by two-step salting-out extraction, which provides a potential method for separation of 1,3-PD, BA and HAc on an industrial scale.
研究领域:
生物工程
·
化学工程
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高影响因子
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