ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Zhihan Tu,Xinyi Mu,Xuemei Chen,Yanqing Geng,Yan Zhang,Qingying Li,Rufei Gao,Taihang Liu,Yingxiong Wang,Junlin He
Dibutyl phthalate (DBP), one of the most widely used plasticizers , is a known environmental endocrine disruptor that impairs male and female fertility. In this study, oral administration of DBP was given to pregnant mice on 14.5 days post coitus (dpc) for 3 days; and additionally, DBP was added into the culture of 14.5 dpc fetal ovaries for 3 days. DBP exposure during gestation disturbed the progression of meiotic prophase I of mouse oocytes , specifically from the zygotene to pachytene stages. Meanwhile, the DBP-exposed pachytene oocytes showed increased homologous recombination sites and unrepaired DNA damage. Furthermore, DBP caused DNA damage by increasing oxidative stress , decreased the expression of multiple critical meiotic regulators, and consequently induced oocyte apoptosis. Moreover, the effect of DBP on meiosis I prophase involved estrogen receptors α and β. Collectively, these results demonstrated a set of meiotic defects in DBP-exposed fetal oocytes. As aberrations in homologous recombination can result in aneuploid gametes and embryos, this study provides new support for the deleterious effects of phthalates.
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PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Xiao-Jing Liao,Hui-Jin Xiao,Jun-Tao Cao,Shu-Wei Ren,Yan-Ming Liu
Photoelectrochemical (PEC) immunoassay is a burgeoning and promising bioanalytical method . However, the practical application of PEC still exist some challenges such as the inevitable damage of biomolecules caused by the PEC system and the unsatisfactory sensitivity for biomarkers with low abundance in real sample. To solve the problems, we integrated the cosensitized structure of Ag2S/ZnO nanocomposities as photoelectrode with photogenerated hole-induced chemical redox cycling amplification (CRCA) strategy to develop a split-type PEC immunosensor for cardiac troponin I (cTnI) with high sensitivity. Initially, the immunoreaction was carried out on the 96-well plates in which alkaline phosphatase (ALP) could catalyze ascorbic acid 2-phosphate (AAP) to generate the signal-reporting species ascorbic acid (AA). Subsequently, the AA participated and the tris (2-carboxyethyl) phosphine (TCEP) mediated chemical redox cycling reaction took place on the photoelectrode, thus leading to signal amplification. Under the optimized conditions, the immunosensor demonstrated a detection limit (LOD) of 3.0 × 10 −15 g mL −1 with a detection range of 1.0 × 10 −14 g mL −1 to 1.0 × 10 −9 g mL −1 for cTnI. Impressively, the proposed method could determine the cTnI in human serum samples with high sensitivity and satisfactory accuracy. Considering the virtues of the photoelectrode and the chemical redox cycling strategy, the method would hold great potential for highly sensitive biosensing and bioanalysis .
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PubMed
高影响因子
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2020
·
IF 0
Xue-Wen Liu,Ning-Yi Liu,Yuan-Qing Deng,Shan Wang,Ting Liu,Yu-Cai Tang,Yuan-Dao Chen,Ji-Lin Lu
Ruthenium(II) complexes containing phenazine ring have attracted attention to design as ‘molecular light switches’. In this study, we synthesized two ruthenium complexes containing phenazine ring and studied their abilities to function as DNA intercalators, DNA ‘light switches’, DNA topo I inhibitors, DNA photocleavers and potential antitumor reagents. [Ru(bpy)2(mbipz)](PF6)2 (1) (bpy = 2,2′-bipyridine, mbipz = 2-(4′-methyl-bipyridine-4-yl)-1H-imidazo[4,5-b]phenazine) exhibited off–on type DNA ‘light swtich’ behavior. DNA binding modes of the two complexes were determined as intercalation by using UV–vis spectra, emission spectra, viscosity and molecular docking experiments. DNA photocleavage experimental results showed that the two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they displayed good topo I inhibition activities. We further found that the two complexes displayed good antitumor activities against Eca-109 cells and A549 cells. The results demonstrated that introduction of phenazine ring will be helpful to design DNA ‘light switch’ based on ruthenium complex.Communicated by Ramaswamy H. Sarma
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PubMed
MOLECULES
·
2018
·Vol. 23
·期 12
·3369
·
IF 3.098
Li-Min Zhao,Zhen Guo,Yi-Jie Xue,Jun Zhe Min,Wen-Jing Zhu,Xiang-Yu Li,Hu-Ri Piao,Cheng Hua Jin
The transforming growth factor-β (TGF-β), in which overexpression has been associated with various diseases, has become an attractive molecular target for the treatment of cancers. Thirty-two quinoxaline-derivatives of 3-substituted-4-(quinoxalin-6-yl) pyrazoles14a–d,15a–d,16a–d,17a–d,18a–d,19a–d,25a,25b,25d,26a,26b,26d,27b, and27dwere synthesized and evaluated for their activin TGF-β type I receptor kinase and p38α mitogen activated protein (MAP) kinase inhibitory activity in enzymatic assays. Among these compounds, the most active compound19binhibited TGF-β type I receptor kinase phosphorylation with an IC50value of 0.28 µM, with 98% inhibition at 10 µM. Compound19balso had good selectivity index of >35 against p38α MAP kinase, with 9.0-fold more selective than clinical candidate, compound3(LY-2157299). A molecular docking study was performed to identify the mechanism of action of the synthesized compounds and their good binding interactions were observed. ADMET prediction of good active compounds showed that these ones possess good pharmacokinetics and drug-likeness behavior.Keywords:TGF-β;ALK5 inhibitor;kinase assay;selectivity;docking
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高影响因子
INORGANICA CHIMICA ACTA
·
2022
·
IF 3.118
Xin Yan,Jia-Qian Chen,Mao-Lin Hu,Hiroshi Sakiyama,Mohd. Muddassir,Jian-Qiang Liu
Two different silver(I) complexes with diphenylphosphonic acid as peripheral ligand were designed and synthesized by using different solvents. The complexes were characterized by X-ray photoelectron spectroscopy (XPS), infrared spectroscopy (IR), elemental analysis (EA), thermogravimetric analysis (TG). Single crystal X-ray analysis established the final structure of both complexes. The interaction modes of the two clusters with DNA were investigated by fluorescence and electrochemical analysis. The theoretical result was justified with the help of theoretical data. The results established that the cluster with methanol solvent was more stable binding with the DNA and protein than the cluster with DMF molecule.
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高影响因子
KINETICS AND CATALYSIS
·
2013
·Vol. 54
·期 6
·716-723
·
IF 0.543
Zeng Minfeng,Sun Xudong,Qi Chenze,Zhang Xian-Man
A novel and environmentally friendly heterogeneous catalyst has been prepared through the wet impregnation of copper(I) species onto the highly porous chitosan microspheres (CuI/PCMS). This novel CuI/PCMS heterogeneous catalyst with no additional ligands is much better than CuI homogeneous catalysts when applied in Heck reactions. Moreover, the reaction conditions are much milder than those for the similar copper catalysts mediated cross-coupling reactions reported in the literature. PCMS has been demonstrated not only the excellent solid support, but also have many unique functional groups to chelate the copper (I) species to enhance its catalytic activity.
研究领域:
催化化学
·
有机合成
·
材料化学
·
环境友好化学
·
功能高分子材料
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高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2024
·
IF 3.2
Shu Li,Zhanmin Cao,Zhongnan Guo,Wenxia Yuan,Fan Sun,Hongmei Wang
This article reports a new perovskite compound Ca 6 Sb 2 O 11 . The X-ray powder diffraction technique was used to analyze its crystal structure. The results show that Ca 6 Sb 2 O 11 has a tetragonal structure with the I 4/ m space group, and the lattice parameters are a = b = 5.658 (1) Å, c = 7.984 (1) Å, α = β = γ = 90°. It exhibits a typical A 4 B' 2 B″ 2 X 12- x anoxic multi-perovskite structure with rock-salt ordering of the B-site cations. The compound's chemical composition and valence states were characterized through scanning electron microscopy-energy dispersion spectrum (SEM-EDS) and X-ray photoelectron spectroscopy (XPS). The presence of oxygen vacancies was confirmed by electron paramagnetic resonance (EPR) spectroscopy. A chemical and structure transition temperature and the full melting temperature of Ca 6 Sb 2 O 11 were measured, with values of 1515 ± 3 K and 1720 ± 3 K, respectively. The density functional theory (DFT)-electron structure calculation results show that Ca 6 Sb 2 O 11 is an indirect wide-band gap (∼4.47 eV) semiconductor material , which agrees well with the experimental value (∼4.38 eV) obtained through diffuse reflection spectroscopy (DRS). Moreover, the top of the valence band and bottom of the conduction band are mainly contributed to by O-2p and Ca-3d states, respectively.
研究领域:
材料科学
·
固体化学
·
计算物理
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高影响因子
APPLIED CLAY SCIENCE
·
2021
·
IF 5.467
Lin Yuan,Yufeng Zhou,Ze Wang,Enrou Mei,Xiaojuan Liang,Weidong Xiang
Colloidal lead halide perovskite nanocrystals (NCs) have recently emerged as versatile photonic sources. However, their performance stability has been far from the requirements, and this type of material is toxic to the environment after degradation. In this paper, a hot injection strategy was used to prepare a CsPbX 3 perovskite NC solution, wherein the photoluminescence quantum yield (PLQY) of CsPbBrI 2 reached 78% and that of CsPbBr 3 was 63%. Then, CsPbX 3 NCs are successfully adsorbed on natural layered mineral pyrophyllite for improving heat, light, and water stability. Monodispersed CsPbX 3 NCs are evenly distributed on the surface of the pyrophyllite sheet in a high-density manner, which possibly originates from the electrostatic attraction effect of abundant anions on the surface of pyrophyllite to CsPbX 3 NCs. In a series of CsPbX 3 @pyrophyllite nanocomposites prepared, the PLQYs of green-emitting CsPbBr 3 and red-emitting CsPbBrI 2 were 47% and 29%, respectively. The materials were employed to fabricate white light-emitting diodes (WLEDs) with a wide color gamut of 117%. Importantly, because of the swelling and cation-adsorbent properties of pyrophyllite, the dissolution of lead in water was greatly reduced, which plays a certain role in ecological protection.
研究领域:
材料科学
·
环境科学
·
光学工程
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高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Fei He,Wenjie Li,Lijuan Yang,Zhen Zhu,Liang Zhou,Xin Zhang,Xiaodong He
Unidirectional alumina aerogels (AAs) were prepared by simultaneously using boehmite hydrosols and alumina nano-powder as solid solutes at different freezing temperatures. In order to understand the porous structural characteristics under different freezing parameters, the real-time frozen parameters were recorded at the designated positions under different freezing temperatures. Fourier transform infrared (FT-IR) spectra, X-ray diffraction (XRD), mercury intrusion porosimetry (MIP) and scanning electron microscopy (SEM) were used to investigate the functional groups, phase composites, microstructures and porous characteristics. The regularities and influence factors of unidirectional porous structures of AAs were detailedly explored under different frozen parameters. The results show that the AAs are characterized with unidirectional porous structures at the direction of ice-crystal growth. The cooling rate and ice front rate ( v inf ) increase and the structure wavelengths ( λ s) decrease with the decrease of the freezing temperature. The relationship between λ and v inf approximately satisfies a power law, which perhaps is related to the uniform dilute solution containing nanoparticle .
研究领域:
材料科学
·
化学工程
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Qiangyu Zhao,Zhenyuan Zhao,Renzhi Rao,Yang Yang,Songyuan Ling,Fukun Bi,Xiaoyu Shi,Jingcheng Xu,Guang Lu,Xiaodong Zhang
In this paper, a simple solvothermal synthesis method was proposed for the preparation of metal organic framework/graphene oxide hybrid nanocomposite (UiO-67/GO). A series of UiO-67/GO composites were prepared by varying the addition forms and amounts of GO, and the optimal synthesis conditions were screened. The composites were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR), transmission Electron Microscope (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopic (XPS), water contact angles (CA) and thermogravimetric analysis (TGA). The adsorption capacity and the adsorption process of toluene were investigated by dynamic adsorption and adsorption kinetics , respectively. The results indicated that 67/GO-0.5% reached the maximum adsorption capacity (876 mg g −1 ), which far exceeded the other adsorbents. Kinetic model and the Weber-Morris model correlated satisfactorily to the experimental data. The improved adsorption performance was attributed to GO, which enhanced π-π interaction, promoted defect generation and provided more adsorption sites. Finally, the excellent regeneration performance of the adsorbent was verified by temperature programmed desorption (TPD) and cyclic adsorption–desorption experiments. Moreover, the adsorption mechanism was further revealed. Combined with the related adsorption experiments and the density functional theory (DFT) analysis, the efficient removal of toluene by UiO-67/GO was attributed to the cooperation of defects, π-π interaction and hydrogen bonding .
研究领域:
材料科学
·
环境科学
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Scientific Reports
·
2026
·
IF 4.9
Zu Kaiyang,Xue Jionghao,Wang Yicong,Li Zhonghan,Gao Jianping,Chen Yu,Luo Xi,Ju Lili,Wang Hao
The study aimed to characterize the regional histological architecture, collagen type I/III composition, and biomechanical properties of human dura mater to provide insights for the biomimetic design of dural repair substitutes. Dura from nine formalin-fixed cadavers were harvested from four anatomical regions: the cranial calvaria, skull base, cervical enlargement, and lumbosacral enlargement. Histological architecture and collagen type I/III composition were assessed using H&E and picrosirius red staining; elastic fibers were visualized with aldehyde fuchsin staining. Collagen type I/III contents were quantified by LC–MS/MS. Mechanical properties, including thickness, ultimate tensile strength, elastic modulus, elongation at break, and toughness, were determined by uniaxial tensile testing. Prototype patches were fabricated using a collagen type I/III blend and mechanically compared against those made from type I collagen alone using uniaxial tensile testing. Cranial dura exhibited four to five interwoven collagen lamellae, whereas spinal dura comprised three predominantly longitudinal layers. Low levels of collagen III were detected in both cranial and spinal dura. Cranial samples were dominated by collagen I with lower collagen III/I ratio, whereas spinal dura showed a relatively higher collagen III/I ratio, mainly attributable to lower collagen I content rather than an increase in absolute collagen III content. Elastic fibers were sparse in cranial dura, least abundant at the skull base, and most abundant in the lumbosacral region. Tensile tests showed that spinal dura had higher elastic modulus than the calvaria. The lumbosacral region exhibited greater tensile strength, elongation at break, and toughness than the calvaria, and higher tensile strength than the skull base. Correlation analyses suggested regional collagen subtype ratio and elastic fiber abundance were associated with elastic modulus. In the preliminary proof-of-concept tensile comparison, patches incorporating 20% type III collagen exhibited increased elongation at break and higher ultimate tensile strength than type I collagen-only control patches. Human dura mater displays pronounced site-dependent structure-composition-mechanics relationships. Regional variations in architecture, collagen type I/III ratio and elastin abundance help explain the distinct mechanical characteristics in cranial and spinal dura. The findings provide a basis for the design of biomimetic dural graft to improve repair outcomes.
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Qiushu Du,Peipei Wang,Zhen Song,Xiliang Luo,Shaoping Lv
Electrochemical biosensors often suffer from biofouling in complex biological samples, which compromises detection accuracy and limits their practical applicability. To address this problem, we developed a synergistic antifouling biosensor by integrating the bovine serum albumin hydrogel (BSAG) with a designed multifunctional peptide for the detection of cardiac troponin I (cTnI) in complex biofluids. A stable and versatile antifouling layer was constructed via a facile one-step crosslinking method based on bovine serum albumin (BSA), and the amino-enriched surface of BSA enabled the secondary grafting of a multifunctional peptide that combines with anchoring, antifouling, and recognition properties. This approach not only enhanced the antifouling performance and durability of the coating, but also avoided potential interference associated with the introduction of exogenous recognition molecules. The cooperative antifouling performance of this hierarchical interface was systematically validated through electrochemical measurements and fluorescence imaging, demonstrating excellent resistance to biofouling in various biological fluids. The fabricated biosensor possessed long-term stability, high sensitivity and a broad linear response was obtained within the range of 0.1 pg mL −1 - 100 ng mL −1 , accompanied by a low detection limit of 0.042 pg mL −1 . Overall, this study presents a robust and generalizable interface engineering strategy that significantly enhances biosensor antifouling performance and analytical reliability, highlighting its strong potential for clinical monitoring in complex biological environments.
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