SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xinhui Hou,Yifan Song,Hengquan Zhou,Lei Guo,Guiying Li,Qian Tao
A novel turn-on fluorescent sensor for hydrogen peroxide (H 2 O 2 ) was prepared from chitosan (CS) coating mesoporous silica nanoparticles (MSNs) loaded with 1-(4-Aminophenyl)-1,2,2-triphenylethene (TPE-NH 2 ) and silver nanoparticles (AgNCs). The surface of MSNs was coated by CS as the gatekeeper and the template for loading of AgNCs. Because of the surface plasmon-enhanced energy transfer (SPEET), AgNCs effectively quenched the fluorescence emission of nanoparticles. In the presence of H 2 O 2 , AgNCs can be oxidized to Ag + , resulting in the recovery of fluorescence. This fluorescent sensor was characterized with respect to its chemical composition, morphological features and optical properties by means of FTIR, XRD, TGA, SEM, TEM, XPS, UV–Vis and fluorescence spectroscopy. The MSN/TPE-CS@Ag nanoparticles showed good sensitivity and selectivity for H 2 O 2 even with various interfering ions and agents. Under optimized conditions, the detection limit for H 2 O 2 was 0.64 μM in the rage of 1–300 μM. The feasibility of the practical application of this probe was confirmed by accurate quantitative of H 2 O 2 in practical samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Zhijie Zhao,Xuzhe Qin,Lilong Cao,Junjie Li,Yuping Wei
Existing anti-swelling hydrogels with poor mechanical strength restrict their underwater human monitoring as wearable electronic sensing equipment. Herein, a nonswelling double network (DN) hydrogel with strong self-recoverability (97.22%) was developed by adding chitosan (CS) to poly(acrylic acid-2-methoxyethyl acrylate)-Fe 3+ [P(AA-MEA)-Fe] network. Owing to the introduction of CS, the hydrogel displayed excellent nonswelling properties under aqueous solutions (pH = 1, 4 and 7), physiological saline, seawater, dodecane, n-hexane and chloroform. Besides, CS improved mechanical properties of hydrogel through non-covalent network (large stretchability of 1199%, tensile strength of 0.462 MPa and toughness of 2.01 MJ/m 3 ). Surprisingly, the hydrogel still reached the extensibility (1072%) and tensile stress (0.467 MPa) even after immersing in water for 7 days. Fabricating hydrogel as flexible strain sensor, periodic real-time signals of human movements (e.g., joint actions and electronic skin touching) were accurately monitored under the water and seawater. The nonswelling P(AA-MEA)-CS-Fe hydrogel shows huge potential in underwater sensing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Gels
·
2022
·Vol. 8
·期 5
·314
·
IF 4.432
Yang Yang,Zixun Wang,Yuanyuan Xu,Jingjing Xia,Zhaoxian Xu,Shuai Zhu,Mingjie Jin
Collagen and chitosan are frequently used natural biomaterials in tissue engineering. However, most collagen is derived from animal tissue, with inconsistent quality and pathogen transmittance risks. In this context, we aimed to use a reliable Type-III recombinant human collagen (RHC) as an alternative biomaterial together with chitosan to develop novel photo-responsive bioinks for three-dimensional (3D) bioprinting. RHC was modified with methacrylic anhydride to obtain the RHC methacryloyl (RHCMA) and mixed with acidified chitosan (CS) to form composites CS-RHCMA. The characterizations demonstrated that the mechanical properties and the degradation of the bioinks were tunable by introducing the CS. The printabilities improved by adding CS to RHCMA, and various structures were constructed via extrusion-based 3D printing successfully. Moreover, in vitro tests confirmed that these CS-RHCMA bioinks were biocompatible as human umbilical vein endothelial cells (HUVECs) were sustained within the constructs post-printing. The results from the current study illustrated a well-established bioinks system with the potential to construct different tissues through 3D bioprinting.Keywords:chitosan;recombinant human collagen;bioinks;3D printing;tissue engineering
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Tongyao Lin,Jinglin Zhang,Hui Long,Fengjuan Yang,Langhuan Huang,Sui-Ping Deng,Jingxian Zhang,Xiang Cai,Yunhua Yang,Shaozao Tan
To improve the environmental response and drug loading/release performance of hydrogels, carboxylated chitosan-modified carbon nanotubes (CCS/CNT) were synthesized as a reinforcer. Moreover, diethylacrylamide (DEA) and N-hydroxymethylacrylamide (NHMAA) acted as a temperature-sensitive monomer and a hydrophilic comonomer, respectively. Thus, a novel micro-nanomodified temperature-sensitive hydrogel (DEA-NHMAA-CNT/CCS) was fabricated via polymerization and cross-linking reactions. The results showed that DEA-NHMAA-CNT/CCS with a uniform and complete pore structure exhibited improved temperature-responsive speed, compression property, and photothermal performance. Furthermore, DEA-NHMAA-CNT/CCS owned an enhanced drug loading/release ability, displaying good temperature-controlled drug release behavior and satisfactory antibacterial activity. The advantages combining facile preparation and good overall performance suggested the great potential application of DEA-NHMAA-CNT/CCS in the fields of drug delivery, wound dressing, and tissue engineering scaffolds.
✓ 已验证
产品关联
DOI
高影响因子
CELLULOSE
·
2022
·Vol. 29
·期 15
·8265-8282
·
IF 6.123
Chaudary Aneeba,Patoary Mohammed Kayes,Zhang Meiling,Chudhary Tayba,Farooq Amjad,Liu Lifang
Aerogels are materials comprising hierarchical designs and are of growing significance to acquire advanced structure-based functionality in future technical applications. Since the researchers have previously focused on a single freeze-casting strategy in the fabrication of aerogels, the correlation between processing routes and their integrated characteristics could not be determined. Herein, we constructed cellulose nanofiber (CNF)/chitosan (CS) based composite aerogels (CCSA) by employing random and directional freeze-casting strategies. The effect of such techniques has been explored as a source of an integrated freeze-cast dried porous structure. Random freeze-dried aerogels (r-CCSA) exhibited isotropic properties with high porosity (99.6%) and large pore volume (2.71 mL/g). Whereas, controlled freeze ice-cast nucleation endowed the directional aerogels with ultra-low thermal conductivity (0.027 Wm −1 K −1 ) in the radial direction and about 20% greater (0.033 Wm −1 K −1 ) in the axial direction. Moreover, high BET-specific surface area (342 m 2 /g) and 60% strain adherence was observed for these anisotropic aerogels. This composite bio-mass aerogel preparation approach can offer a clear insight into the adoption of the right methodology considering the potential applications and environmental safety. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
AAPS PHARMSCITECH
·
2022
·Vol. 23
·期 6
·1-11
·
IF 4.026
Zou Linghui,Zhang Zhongbin,Chen Jinqing,Yang Xu,Li Yuyang,Tang Jing,Du Xiaolu,Tang Ling,Liang Dan,Zhu Xiaoyong,Feng Jianfang,Ding Wenya
β-Cyclodextrin (CD) and chitosan (CS) have attracted great attention due to their unique properties and structures. β-Cyclodextrin-grafted chitosan (CD-CS) has been widely used as a drug carrier to prepare nano-formulations for drug delivery. However, few researches have been conducted to investigate the effect of CD-CS as an excipient on cellular uptake and intestinal absorption. Herein, Caco-2 cells were used to investigate the influence of CD-CS on cellular uptake. The MTT assay showed that CD-CS was non-toxic to Caco-2 cells in concentrations ranging from 15.62 to 125 μg/mL. Confocal laser microscopy and flow cytometry measurements indicated that the uptake ability of Caco-2 cells was significantly enhanced after being treated with CD-CS at a concentration of 31.25 μg/mL or incubation for 0.5 h, and the uptake enhancement gradually increased with increasing CD-CS concentration and incubation time. The Caco-2 monolayer cell model and the everted intestinal sac method were employed to preliminarily explore the mechanism of the improved intestinal absorption. The results demonstrated that CD-CS might open the tight junctions and enhance the clathrin-dependent endocytosis, macro-pinocytosis, and phagocytosis of the intestinal epithelial cells. Such findings can serve as references and inspiration for the design of absorption enhancers. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Tao Liu,Zhuoran Zhang,Jiacheng Liu,Peijie Dong,Feng Tian,Fan Li,Xin Meng
Development of chitosan-based hemostatic products and their application in wound healing has always been a research hotspot in the field of emergency treatment and biomedicine . In this study, the classic hemostatic chitosan and the most well-known inorganic hemostatic agent—kaolin were tried to combine to form a more excellent dressing. Together with the aid of non-toxic, harmless and good hydrophilic polyethylene oxide, chitosan/polyethylene oxide (PEO)/kaolin nanofiber membranes (CPKs) were prepared by electrospinning technology. Such membranes exhibited adjustable mechanical properties and good biocompatibility. Furthermore, a series of in vitro coagulation experiments proved that CPKs with 10 % ratio of kaolin (CPK10) has excellent hemostatic ability. Especially, in the whole blood coagulation time (WBCT) assay, the hemostatic time of CPK10 (43 ± 1.4 s) was significantly lower than that of chitosan/polyethylene oxide (CPK0) nanofiber membrane (61 ± 2.2 s) and QuikClot® Combat Gauze (55.7 ± 1.2 s). The further rat liver injury test reconfirmed that CPK10 can stop bleeding better and faster compared to other groups. In addition, CPKs could promote back wound healing in rats within 14 days without significant inflammatory response. This safe and effective hemostatic CPKs is expected to be a promising candidate hemostat in pre-hospital medical care.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
·
2022
·
IF 2.712
Junjing Zhou,Xinglian Wu,Chaochen Zhao
Hepatocyte scaffold is an essential part in bioartificial liver device. We have designed a novel hepatocyte scaffold based on porcine liver extracellular matrix (ECM) and chitosan (CTS) fabrics. This CTS-ECM scaffold can improve cell adhesion and proliferation. In the present study, an orthogonal test was designed to optimize the CTS/ECM composite scaffold, in which ECM concentration, EDC concentration and EDC to NHS ratio were taken as factors, proportion of nitrogen element and hydroxyproline content as indicators. The cytocompatibility of the novel scaffold for C3A hepatocytes was analyzed in vitro. The orthogonal test demonstrated that the optimal scaffold should be based on ECM concentration of 5 mg/mL, EDC concentration of 5 mg/mL, and EDC to NHS ratio 1:1. C3A hepatocytes cultured on the optimized CTS-ECM scaffolds showed stronger proliferation and functionality than those on Cytodex3 microcarriers (p < 0.05). The CTS/ECM composite scaffold may be widely used as a promising hepatocyte culture carrier, especially in bioartificial liver support systems.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
·
2022
·
IF 3.221
Yuting Zhang,Chenlu Dai,Jiugang Yuan,Ping Wang
Maintaining a moist wound environment facilitates the wound healing process. However, traditional dressings such as medical gauze are difficult to meet this condition and easy to cause wound infection when the gauze is replaced. In this paper, a novel multifunctional medical dressing based on keratin porous materials was prepared by the freeze-drying method. Keratin was first modified by poly (ethylene glycol) dimethacrylate (PEGDMA) to increase its hydrophilicity. Then, the carboxymethyl chitosan (CMCS) was mixed with keratin solution to further improve the pore-forming properties, and finally, the solution was dried through the freezing method to obtain the porous dressing. The performance of the dressing was detailed and studied using DPPH free radical scavenging ability, water retention, blood coagulation, and bacteriostasis tests. The results showed that PEGDMA grafting greatly improved moisture retention from 22 to 43. CMCS blending not only improved the uniform and compact of the pore structure, but also effectively improved blood coagulation and antibacterial properties. After blending with CMCS, the performance of the keratin porous material was greatly improved. Carboxymethyl chitosan is positively charged, which makes the negatively charged platelets easy to aggregate, so the coagulation performance is greatly improved, and the BCI decreased to 15%. At the same time, the antibacterial performance against Staphylococcus aureus was greatly improved, and the optimal condition was PEG-g-KR:CMCS = 6:4. The PEG-g-KR/CMCS dressing prepared in this method had the characteristics of promoting wound healing, good moisture absorption and water retention, excellent blood coagulation performance and strong bacteriostasis. It is of great significance to the research and application of composite dressings.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Xiaoli Zhang,Qingli Qu,Anquan Yang,Jing Wang,Weixia Cheng,Yankang Deng,Aying Zhou,Tao Lu,Ranhua Xiong,Chaobo Huang
The emergence, spread and difficult removal of bacteria biofilm, represent an ever-increasing persistent infections and medical complications challenge worldwide. Herein, a self-propelled system Prussian blue micromotor (PB MMs) were constructed by gas-shearing technology for efficient degradation of biofilms by combining chemodynamic therapy (CDT) and photothermal therapy (PTT). With the interpenetrating network crosslinked by alginate , chitosan (CS) and metal ions as the substrate, PB was generated and embedded in the micromotor at the same time of crosslinking. The micromotors are more stable and could capture bacteria with the addition of CS. The micromotors show excellent performance, containing photothermal conversion, reactive oxygen species (ROS) generation and bubble produced by catalyzing Fenton reaction for motion, which served as therapeutic agent could chemically kill bacteria and physically destroy biofilm. This research work opens a new path of an innovative strategy to efficiently remove biofilm.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2021
·Vol. 10
·期 12
·3003
·
IF 4.35
Kaibo Yu,Jing Xu,Lei Zhou,Liqiang Zou,Wei Liu
Mango (Mangifera indicaLinn.) is a famous climacteric fruit containing abundant flavor and nutrients in the tropics, but it is prone to decay without suitable postharvest preservation measures. In this study, the chitosan (CH)-cinnamon essential oil (CEO) Pickering emulsion (CH-PE) coating was prepared, with cellulose nanocrystals as the emulsifier, and applied to harvested mangoes at the green stage of maturity. It was compared with a pure CH coating and a CH-CEO emulsion (CH-E) coating, prepared with the emulsifier Tween 80. Results showed that the CH-PE coating had a lower water solubility and water vapor permeability than the other coatings, which was mainly due to electrostatic interactions, and had a better sustained-release performance for CEO than the CH-E coating. During mango storage, the CH-PE coating effectively improved the appearance of mangoes at 25 °C for 12 d by reducing yellowing and dark spots, and delayed water loss. Hardness was maintained and membrane lipid peroxidation was reduced by regulating the activities of pectin methyl esterase, polygalacturonase, and peroxidase. In addition, the nutrient quality was improved by the CH-PE coating, with higher contents of total soluble solid, titratable acid, and ascorbic acid. Therefore, the CH-PE coating is promising to comprehensively maintain the postharvest quality of mangoes, due to its enhanced physical and sustained-release properties.Keywords:emulsion;cinnamon essential oil;edible coating;mango;postharvest quality
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2021
·
IF 3.383
Jun-Lan Yang,Hou-Qun Yuan,Bao-Sheng Liu,Jia-Xin He,Qing Fan,Ke Deng,Deping Song,Guang-Ming Bao
Enrofloxacin is a broad spectrum antibacterial agent with an excellent killing effect against bacteria, commonly infecting the respiratory and intestinal tracts of livestock and poultry. However, its bitter taste and significant irritation to the gastrointestinal tract often lead to a failure of the commonly used current drug delivery system to achieve the desired therapeutic effect due to inefficient release of drugs. In the present study, pH-responsive enrofloxacin nanoparticles ( ENR-NPs ) for treating bacterial infections were fabricated through a facile one-pot approach to achieve the amphiphilic functionalization of chitosan (CS) through acid-labile Schiff-base with cinnamaldehyde and drug encapsulation. The structure and morphology of ENR-NPs were characterized by FTIR, X-Ray Diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). The ENR-NPs were uniform in size and full in shape with an average particle size of 135 nm. The encapsulation rate (76.61%) and drug loading capacity (3.3%) were measured by ultraviolet spectrophotometry . The in vitro experiments elucidated that the release rate of enrofloxacin from ENR-NPs was significantly faster at pH 5.0 than at pH 7.4 due to the cleavage of the acid-sensitive Schiff-base bond. Further, the antimicrobial studies revealed that the inhibitory effects were improved with the increase of acidity of the culture medium. Therefore, ENR-NPs provide a potential application of enrofloxacin for bacterial-response drug delivery.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）