JOURNAL OF MOLECULAR LIQUIDS
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2018
·
IF 4.513
Yue Zhang,Caili Dai,Xuepeng Wu,Yining Wu,Yuyang Li,Yongping Huang,Haonan He,Chenglin Gu,Long He,Mingwei Zhao
To further investigate the interaction between surface-modified nanoparticles and wormlike micelles (WLMs) and their rheological response, a newly kind of anhydride-modified silica nanoparticles (AMSNPs) was modified by succinic anhydride . A quaternary ammonium surfactant with the headgroup modified by two hydroxyethyl groups N -cetyl- N , N -di(2-hydroxyethyl)methylammonium bromide (CDHAB) was synthesized in this work. The transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used to characterize AMSNPs. The formation of WLMs by CDHAB and sodium salicylate (NaSal) was observed by cryogenic transmission electron microscopy (cryo-TEM). With the addition of nanoparticles, rheological properties of WLM solution get enhancement remarkably. Furthermore, compared with the aid of normal silica nanoparticles (SNPs), WLMs with AMSNPs show higher viscosity and better viscoelasticity . This can be attributed to the high ionization degree of AMSNPs in the nanofluid and stronger electrostatic attraction and extra hydrogen bonding interaction between functional groups of AMSNPs and CDHAB, leading to more micelle-nanoparticle junctions and longer micelles. This work can give further view and knowledge of interaction between functional groups of modified nanoparticles and special surfactants of WLMs.
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Soft Matter
·
2019
·Vol. 15
·期 38
·7644-7653
·
IF 3.399
Yugui Han,Yefei Wang,Xianghai Meng,Qiuxia Wang,Xiaodong Han
Wormlike micelles (WLMs) have been successfully constructed from many different C22-tailed surfactants. Here, we creatively introduced a bulky piperazine group onto a C22-tailed zwitterionic surfactant, N-erucamidopropyl-N,N-piperazine-N-methyl ammonium propanesulfonate (EDPS), and investigated the micellar structure and properties of the EDPS WLMs via molecular dynamics simulation, cryo-TEM and rheological techniques. It was found that 25 mM EDPS increased the zero-shear viscosity to as high as ∼106 mPa s. Furthermore, abnormal rheological behaviors, such as an inflection in the shear thinning region of steady rheology and an abrupt decrease of the shear stress at a critical shear rate, were observed, which was attributed to the unique ladder shape micellar structure. The EDPS WLMs were superior to other C22-tailed surfactants in many aspects, such as a low overlapping concentration, higher viscosity, stable viscosity over the whole pH range, and great temperature and salt (NaCl, CaCl2 and MgCl2) tolerance.
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高影响因子
COLLOID AND POLYMER SCIENCE
·
2018
·Vol. 296
·期 3
·495-502
·
IF 1.967
Zhang Rongli,Xu Xiaofang,Fan Xiaoxia,Yang Renchun,Wu Tao,Zhang Cuige
A novel kind of biocompatible conducting micelles self-assembled from commercial poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) and chitosan (CS) via electrostatic interaction. The resultant micelles were then used to provide a platform for fabricating high sensitive biosensor. It is expected that the introduction of chitosan in micelles can solve the problem of PEDOT:PSS film easily peeling off from the surface of the electrode. The micelles with nanostructure on the electrode can facilitate the immobilization of enzyme due to the large surface to volume ratio. The good conductivity of the commercial PEDOT:PSS can provide a platform to accelerate the electron transfer and facilitate the signal transduction from the micelles film to the underlying electrode. These advantages endowed the biosensor with high sensitivity for H 2 O 2 detection. Under the optimal conditions, a linear ranged from 1 × 10 −10 to 1 × 10 −5 mol·L −1 with a detection limit of 3 × 10 −11 mol·L −1 were obtained for H 2 O 2 detection. The biosensor can be used to analyze H 2 O 2 in commercial disinfector with satisfactory results.
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CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Shulong Jiang,Mengying Li,Ying Hu,Zhenhai Zhang,Huixia Lv
A polymer of Galactosamine-hyaluronic acid-Vitamin E succinate (Gal-HA-VES) was designed to prepare multifunctional self-assembled micelles for delivery of Norcantharidin (NCTD) to Hepatic carcinoma. NCTD/Gal-HA-VES showed higher cytotoxicity toward CD44-overexpressing MCF-7 cells, MCF-7/Adr cells and ASGP-R overexpressing HepG2 cells, consistent with the enhanced cellular uptake in the selected cell models, indicating Gal-HA-VES micelles were taken up in MCF-7 and HepG2 cells by CD44 and ASGPR receptor mediated endocytosis, respectively. Moreover, the accumulation of Rhodamine 123 demonstrated that Gal-HA-VES has the same action of TPGS as a P -glycoprotein inhibitor blocked drug efflux-related MDR mechanism in resistant MCF-7/Adr cells. The Cell apoptosis assays indicated that NCTD/Gal-HA-VES were more effective in triggering apoptosis, compared with free NCTD or NCTD/HA-VES groups. In vivo study demonstrated that NCTD/Gal-HA-VES group exhibited enhanced tumor targeting and antitumor activity with lower systemic toxicity. Hence NCTD/Gal-HA-VES micelles system can achieve significant tumor targeting and effective treatment of hepatic carcinoma.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2018
·
IF 2.297
Xiaoling Wang,Junbo Wang,Junjun Li,Hongxia Huang,Xiaoyi Sun,Yuanyuan Lv
To enhance the effectiveness and specificity of photodynamic therapy (PDT) in CD44-overexpressing cancers, the hyaluronic acid-b-poly (d,l-lactide-co-glycolide) copolymer (HA-b-PLGA) was synthesized, and potent photosensitizer protoporphyrin IX (PpIX) loaded polymeric micelles were prepared. The formulation organic solvent, polymer concentration, and feeding weight ratio of PpIX to polymer were optimized. PpIX-loaded HA-b-PLGA micelles (PpIX HA-PLGA micelles) were obtained with a drug loading rate of 2%, encapsulation efficiency of 43%, average diameter of 213 nm and zeta potential of −24 mV. The PpIX HA-PLGA micelles exhibited enhanced phototoxicity toward A549 cells. A cellular uptake study indicated that the micelles were internalized by CD44-mediated endocytosis. Exposure of A549 cell spheroids to HA-PLGA micelles showed deeper penetration and improved phototoxicity compared with nanoparticle and free drug analogues. PpIX HA-PLGA micelles have great potential for CD44-targeting delivery in PDT treatment.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2018
·Vol. 30
·期 1
·40-50
·
IF 2.137
Feng Su,Xiangke Sun,Rongye Li,Yuandou Wang,Laishun Xi,Yangsheng Chen,Suming Li
A series of poly(ɛ-caprolactone/glycolide)-poly(ethylene glycol) (P(CL/GA)-PEG) diblock copolymers were prepared by ring opening polymerization of a mixture of ɛ-caprolactone and glycolide using mPEG as macro-initiator and stannous octoate as catalyst. Self-assembled micelles were prepared from the copolymers using nanoprecipitation method. The micelles were spherical in shape. The micelle size was larger for copolymers with longer PEG blocks. In contrast, the critical micelle concentration of copolymers increased with decreasing the overall hydrophobic block length. Drug loading and drug release studies were performed under in vitro conditions, using paclitaxel as a hydrophobic model drug. Higher drug loading was obtained for micelles with longer poly(ε-caprolactone) blocks. Faster drug release was obtained for micelles of mPEG2000 initiated copolymers than those of mPEG5000 initiated ones. Higher GA content in the copolymers led to faster drug release. Moreover, drug release rate was enhanced in the presence of lipase from Pseudomonas sp., indicating that drug release is facilitated by copolymer degradation. The biocompatibility of copolymers was evaluated from hemolysis, dynamic clotting time, and plasma recalcification time tests, as well as MTT assay and agar diffusion test. Data showed that copolymer micelles present outstanding hemocompatibility and cytocompatibility, thus suggesting that P(CL/GA)-PEG micelles are promising for prolonged release of hydrophobic drugs.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Zhongcheng Ke,Lei Yang,Hao Wu,Zihao Li,Xiaobin Jia,Zhenghai Zhang
This study aimed to develop a novel type of multilayer micelle using protamine (PRM) and hyaluronic acid (HA) for the delivery of gambogic acid (GA). GA-loaded micelles (GA-M) were simply and rapidly prepared using lecithin/solutol HS15 using a film-dispersion method. PRM and HA were added in sequence to form layer-by-layer self-assembled micelles (HA-PRM-GA-M), in which particle size, zeta potential , particle morphology, drug loading, encapsulation efficiency, and in vitro release were investigated. Surface charge reversal demonstrated that rapid HA detachment exposed PRM, leading to activation of a “proton sponge” effect in the hyaluronidase (HAase)-rich tumor microenvironment . Compared with coumarin 6-loaded micelles (C6-M), more efficient intracellular trafficking was observed for HA-PRM-C6-M, which is associated with the endosomal/lysosomal escaping ability of the exposed PRM. In vivo imaging showed increased enrichment of near infrared fluorescent dye (DIR)-loaded HA-PRM-DIR-M at the tumor site, suggesting that HA enhanced the active tumor targeting of GA. Furthermore, HA-PRM-GA-M showed the stronger antitumor activity than GA and GA-M against human lung adenocarcinoma (A549) tumor xenografts in nude mice . In summary, our findings show the potential of HA-PRM-GA-M as a novel intravenous drug carrier for the treatment of lung cancer.
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Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Wenhao Zhou,Zhaojun Jia,Pan Xiong,Jianglong Yan,Ming Li,Yan Cheng,Yufeng Zheng
To endow orthopaedic implants with satisfactory antibacterial properties, the design and development of antibiotic coating on the surface of implants is highly desired. In this work a novel and facile strategy was developed to form pH-responsive layer-by-layer (LbL) films implanted with polymeric micelles as nano-vehicles loaded with charge-weak antibiotic drugs, enabling high drug loading efficiency. Negatively charged tobramycin (Tob)-embeded heparin miscells (HET) and positively charged chitosan (CHT) were exploited as a pH-responsive LBL multilayer building block, respectively. The formation mechanism and pH-stimulated release behavior of the Tob-contained heparin micelles were studied. The characterization on the morphologies, chemical compositions and hydrophilicity of the modified surface confirmed the successuful deposition of the Tob-loaded CHT/HET multilayers coatings on the polydopamine-modified Ti surface. The drug release profiles displayed fast release at pH 7.4 and slow release after exposure to weakly acidic environments. Antibacterial tests indicated that the Tob-embed CHT/HET nanostructured multilayers not only strongly inhibited initial bacterial adhesion, but also disruptted biofilm formation. Particularly, this functional coatings showed “long-term antibacterial” pattern in acid condition. Meanwhile, MC3T3 cells showed acceptable adhesion, spread and proliferation on the multilayer coatings in cytocompatible studies. In a word, these multilayer coatings incorporated with a wide variety of antibiotics show promisiong applications in preventing postoperative infection and resolving unmet clinical need.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Jilian Wu,Huiyuan Zhang,Xu Hu,Ruiling Liu,Wei Jiang,Zhonghao Li,Yuxia Luan
Clinically, codelivery of chemotherapeutics has been limited by poor water-solubility and severe systemic toxicity. In this work, we developed a new reduction-sensitive mixed micellar system for self-codelivery of doxorubicin (DOX) and docetaxel (DTX). Biodegradable methoxy poly(ethylene glycol)-poly(ε-caprolactone) (mPEG-PCL) was coupled with DOX and DTX by a reduction-sensitive disulfide bond, resulting in mPEG-PCL-SS-DOX and mPEG-PCL-SS-DTX, respectively. mPEG-PCL-SS-DOX was mixed with mPEG-PCL-SS-DTX at a mole ratio of 1:1 in water, forming a mixed micellar system. The mixed micelles had a diameter of 223.7 nm and a low critical micelle concentration. Reductive-triggered drug release revealed a “smart” characteristic of the mixed micelles. A cellular uptake and cytotoxicity assay in vitro showed that the mixed micelles could efficiently accumulate in MCF-7 cells and suppress the growth of tumour cells. The proposed reduction-sensitive mixed micelles assembled from amphiphilic prodrugs can be used as a promising drug codelivery system for cancer therapy.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Xuzhu Zhang,Fang Du,Jin Huang,Wei Lu,Shiyuan Liu,Jiahui Yu
This research is aimed to develop a biodegradable micelle delivery system with sheddable poly (ethylene glycol) shell to achieve the reduction-triggered intracellular sustained release of 6-mercaptopurine (6-MP) and decreased toxicity. Firstly, the amino-disulfide linked poly (ethylene glycol) monomethyl ether (mPEG-SS-NH 2 ) was synthesized by the amidation reaction between cystamine and active ester of mPEG and p-nitrophenyl chloroformate (p-NPC) (mPEG–NPC). And then, the five-member rings in poly ( l -succinimide) (PSI) were successively opened by mPEG-SS-NH 2 and 2-(pyridyldithio)-ethylamine (PDA) to produce the graft copolymer of mPEG-SS-NH- graft -PAsp-PDA. To avoid the drug initial burst, 6-MP was covalently conjugated with mPEG-SS-NH- graft -PAsp-PDA by thoil–disulfide exchange reaction to give the resultant product mPEG-SS-NH- graft -PAsp-MP. The product was found to form spherical micelles in aqueous media because of its amphiphilic nature with average particle size of 160 nm measured by dynamic light scattering (DLS). It was found that the mPEG-SS-NH- graft -PAsp-MP micelles, though stable in phosphate buffer solution (PBS), were prone to aggregation in the presence of dithiothreitol (DTT). The in vitro drug release studies revealed the release of 6-MP were distinct from the conventional micelles whose drugs loaded by physical encapsulation. Sustained release profile of 6-MP over 85 h was found in the presence of DTT (40 mM) simulating the intracellular condition while minimal drug release was observed within 24 h at the level of DTT corresponding to extracellular environment. Remarkably, the cell viability results showed there was essential decrease of cytotoxicity to HL-60 cell line compared to free 6-MP.
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JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2015
·Vol. 54
·期 1
·99-107
·
IF 3.113
Ying Huang,Rui Sun,Qiaojie Luo,Ying Wang,Kai Zhang,Xuliang Deng,Weipu Zhu,Xiaodong Li,Zhiquan Shen
In this study, a facile method to fabricate reduction-responsive core-crosslinked micelles via in situ thiol-ene “click” reaction was reported. A series of biodegradable poly(ether-ester)s with multiple pendent mercapto groups were first synthesized by melt polycondensation of diol poly(ethylene glycol), 1,4-butanediol, and mercaptosuccinic acid using scandium trifluoromethanesulfonate [Sc(OTf) 3 ] as the catalyst. Then paclitaxel (PTX)-loaded core-crosslinked (CCL) micelles were successfully prepared by in situ crosslinking hydrophobic polyester blocks in aqueous media via thiol-ene “click” chemistry using 2,2′-dithiodiethanol diacrylate as the crosslinker. These PTX-loaded CCL micelles with disulfide bonds exhibited reduction-responsive behaviors in the presence of dithiothreitol (DTT). The drug release profile of the PTX-loaded CCL micelles revealed that only a small amount of loaded PTX was released slowly in phosphate buffer solution (PBS) without DTT, while quick release was observed in the presence of 10.0 mM DTT. Cell count kit (CCK-8) assays revealed that the reduction-sensitive PTX-loaded CCL micelles showed high antitumor activity toward HeLa cells, which was significantly higher than that of reduction-insensitive counterparts and free PTX. This kind of biodegradable and biocompatible CCL micelles could serve as a bioreducible nanocarrier for the controlled antitumor drug release. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2016 , 54 , 99–107
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BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2015
·Vol. 20
·期 3
·550-560
·
IF 1.113
Hao Shanhu,Yan Ying,Ren Xue,Xu Ying,Chen Lanlan,Zhang Haibo
A multifunctional polymer-drug conjugate candesartan-graft-polyethyleneimine (CD-PEI, CP) containing low molecular weight polyethyleneimine (PEI) and candesartan (CD) conjugated via an amide bond was fabricated as a co-delivery micelle of drug and siRNA for potential lung cancer therapy. Here, CD as an angiotensin II type 1 receptor blocker rich in imidazole and tetrazole rings was utilized to strengthen endosomal buffering capacity of CP and suppress tumor angiogenesis. The selfassembled CP/siRNA complexes exhibited desirable and homogenous particle size, moderate positive charges, and efficient release of drug and siRNA in vitro . In addition, CD and siRNA could readily detached from nanovectors in tumor cells via an amidase-responsive mechanism and they achieved synergistic anti-angiogenesis efficacy by effectively downregulating the expression of vascular endothelial growth factor (VEGF) mRNA and protein via different pathways in vitro. In vivo investigation on nude mice bearing A549 tumor xenografts revealed that CP/siRNA complexes possessed strong antitumor activity. These findings suggested that CP could be an ideal nanovector for simultaneous transfer of drug and siRNA, and a multifunctional CP/siRNA co-delivery system with enhanced endosomal buffering capacity, amidase-responsive drug release and synergistic anti-angiogenesis efficacy might be a new promising strategy for effective lung cancer therapy.
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