Analytical Methods
·
2024
·Vol. 16
·期 46
·7864-7872
·
IF 2.7
Jing Wang,Lu Ren,Qiang Sun,Yanqi Liu,Wanru Jia,Huihong Zhang,Dawei Zhang
A simple turn-off fluorescent probe, 5-(diethylamino)-2-(hydrazonomethyl)phenol (DHP), is designed and synthesized for the sensitive and selective detection of Cu2+. The bright green fluorescence of DHP is quenched after the addition of Cu2+. The probe DHP exhibits good anti-interference performance against Cu2+ in the presence of multiple metal ions. The fluorescence intensity of DHP (10 μM) at 522 nm is well linearized with the Cu2+ concentration at 0–5 μM, and it has a detection limit of 0.29 nM (R2 = 0.9949). The complexation ratio of the probe DHP to Cu2+ is 2 : 1 and the complexation constant is 3.44 × 104 M−1 (R2 = 0.9974). In addition, the probe DHP can be recovered using EDTA and Cu2+ can be effectively monitored at pH 5–11, with good results in dipstick experiments and actual water samples. HepG-2 cells remained viable in excess of 90% after being exposed to DHP (50 μM) for 24 h, which demonstrates the extremely low toxicity of DHP, and it can be used for in vivo cell imaging.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Zhen Wu,Yi Wei,Yue Tang,Lian Xia,Xiaojuan Niu,Yuangen Wu
Food poisoning caused by excessive intake of histamine is a significant public health concern. Traditional analytical methods struggle to meet the demands for on-site monitoring of histamine in food. Colorimetry based on nanozyme catalysis can be applied to rapidly detect histamine, but some challenges remain in the specific response of nanozymes to histamine. Herein, a novel “off-on” colorimetric sensor was first established for histamine detection based on Cu 2+ -mediated laccase-mimic activity of flower-like Mn 3 O 4 nanoparticles ( Mnf ). This work proved that Cu 2+ can inhibit the laccase-mimic activity by hindering the electron transfer between Mnf and 2,4-DP molecules. Once histamine was present in the system, Cu 2+ was first consumed due to the coordination with histamine, resulting in the recovery of laccase-mimic activity of Mnf . The color of sensing solution can be converted to the corresponding RGB value by a smartphone for on-site detection of histamine in food. The colorimetric sensor has a limit of detection as low as 0.148 μg mL −1 , which is comparable to that of most colorimetric methods. Moreover, the colorimetric sensor exhibits high selectivity, and has considerable applicability for histamine detection in aquatic products.
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Weiwei Qiu,Qingxiang Wang,Natsumi Yano,Yusuke Kataoka,Makoto Handa,Feng Gao,Hidekazu Tanaka
A flexible metal-organic framework (MOF) of Cu 2 ( trans- 1,4 - cyclohexanedicarboxylic acid) 2 (Cu 2 (CHDC) 2 ) has been synthesized by a facile hydrothermal method . The physical characterization experiments show that the Cu 2 (CHDC) 2 presents unique flower-like shape, large surface area and high electroactivity. Then gold nanoparticles (AuNPs)/Cu 2 (CHDC) 2 modified electrode was prepared and utilized as an electroactive matrix for thrombin aptamer immobilization. The electrochemical assay shows that the AuNPs can effectively enhance the electrochemical signal of the Cu 2 (CHDC) 2 . Upon the binding of the electrode-confined aptamer with the thrombin, the electrochemical signal of the Cu 2 (CHDC) 2 decreases obviously due to the change of the microenvironment of the sensing interface. Thus a label-free electrochemical sensing strategy for thrombin was realized. The aptasensor displays excellent performance with wide linear range, ultralow detection limit of 0.01 fM (S/N = 3), and favorable specificity. Also, the aptasensor was applied for determination of thrombin in the human serum samples, and the satisfactory results are obtained. The results imply that the proposed sensor can be potentially used for the early diagnosis of disease and clinical monitoring.
✓ 已验证
DOI
高影响因子
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
·
2021
·
IF 3.995
Wei Tian,Yaqin Rong,Dali Li,Jin Tian,Naiming Lin,Zhongde Wang
The contamination of water by heavy metal ions can easily cause serious environmental problems and damage to the human body hands down. Using zeolitic imidazolate framework-67 (ZIF-67) as a sacrificial template, polyhedral CoS hollow nanocages (HNC) were successfully synthesized by self-template approach as adsorbents for heavy metal ions removal. The CoS HNCs suggested good chemical stability and extremely efficient heavy metal ions removal of Ag + , Cd 2+ , Cu 2+ , Pb 2+ and Zn 2+ , which can be ranked in the order of Ni 2+ ≪ Zn 2+ , Cd 2+ < Pb 2+ , Cu 2+ , Ag + . The saturation adsorption for Ag + reached 2147.944 mg g −1 and exhibited high distribution coefficients (K d ) > 107 mL g −1 . The CoS HNCs can rapidly reduce the Ag + concentration from ppm level to trace level of 1 ppb. It has been proved that the rapid and selective adsorption was achieved via the hierarchical pore structure of the CoS HNCs can better disperse these metal vacancies with super affinity and the S 2− , reduce the mass transfer distance and accelerate mass transfer. It is expected that such a CoS HNCs could be a promising alternative to conventional materials for the adsorption of heavy metal ions.
✓ 已验证
DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 9
·3013-3022
·
IF 4.019
Baogang Wang,Hui Tan,Tailiang Zhang,Wenmeng Duan,Yuanqiang Zhu
N-Doped carbon dots (NCDs) were facilely synthesized by a hydrothermal method using an ethanolamine–ionic liquid (1-carboxyethyl-3-methyl imidazole chloride) gel as a precursor. The NCDs had an average particle size of 3.4 nm and were partially crystalline and abundant in amide and hydroxyl groups and pyridinic/pyrrolic nitrogen atoms on their surfaces. In water, the NCDs showed excitation- and concentration-dependent photoluminescence (PL) properties with a high salt tolerance and a quantum yield of 24.7% under 350 nm excitation. The PL of the aqueous suspension of NCDs remained stable in a pH range of 4–11 and was significantly quenched by Hg2+ and Cu2+ owing to the strong interactions between the metal ions and the surface groups and/or nitrogen atoms of the NCDs. The possible quenching mechanism was determined to be photo-induced electron transfer. By introducing the masking agents of P2O74− and S2O32− into the aqueous suspension of NCDs, two label-free “turn-off” fluorescent probes for Hg2+ and Cu2+ with a high sensitivity and selectivity were built, respectively. Moreover, the quenched NCDs–Hg2+ system could be used as a selective “turn-on” fluorescent probe for S2O32− due to the strong affinity of S2O32− to Hg2+. The above described three probes all illustrated two good linear detection ranges for Hg2+ (0–10 and 10–50 μM), Cu2+ (0–2.5 and 2.5–40 μM) and S2O32− (0–20 and 20–80 μM), and their limits of detection were calculated to be 0.076, 0.125 and 1.17 μM. The recovery tests demonstrated that the above described probes were reliable and capable of detecting corresponding ions in tap water samples with satisfactory results.
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Fan Wang,Yuqing Wang,Lili Zhang,Ting Zheng,Jun Shen
Two novel cellulose phenylcarbamate derivatives bearing 3,5-dimethylphenylcarbamate substituents at 2,3-positions and 4-(4-(3-(2-benzo[ d ]thiazolyl)ureayl)benzyl)) ( Cel-1 ) or 4-(4-(3–4-(2-benzo[ d ]thiazolyl)phenyl)ureayl)benzyl)) ( Cel-2 ) at 6-position of glucose units were prepared. Their metal ion discrimination properties were then carefully scrutinized using 12 metal ions. Combined the 2,3-dimethylphenylcarbamates with a more bulky benzothiazolyl phenylcarbamate at 6-position, the derivative Cel-2 exhibited excellent recognition ability for both Cu 2+ and Fe 3+ , and achieved high selective detection at extremely low concentrations through fluorescence and UV–visible modes. The limit of detection of Cel-2 for the two metal ions in both modes were 2.0 μM (Cu 2+ ) and 1.0 μM (Fe 3+ ) (in fluorescence mode), 1.3 μM (Cu 2+ ) and 0.7 μM (Fe 3+ ) (in UV–visible mode), respectively, which were much lower than the maximum pollution level for copper ions (20 μM) and iron ions (5.4 μM) in the potable water. In addition, good anti-interference ability of Cel-2 was also observed for specific detection of Cu 2+ /Fe 3+ in the combined system containing other metal ions. It revealed that the obtained regioselectively substituted phenylcarbamate of cellulose possessed powerful performance for efficient detection of Cu 2+ /Fe 3+ in various circumstance via fluorescence and UV–visible modes. Furthermore, Cel-2 was also been prepared into smart ink, presenting its possible potential in the anti-counterfeiting application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Jialing Zhuang,Shunyu Chen,Xiufeng Xiao
3D-printed gelatin scaffolds are widely explored for bone regeneration due to their excellent biocompatibility and biodegradability, yet their clinical translation is severely hindered by several inherent defects, including weak mechanical stability, fast in vivo biodegradation, limited osteogenic capability, and the absence of anti-infective functions. Globally, it remains a key challenge in bone tissue engineering to develop integrated scaffold systems that simultaneously satisfy mechanical matching, long-term biological activity, and anti-pathogenic requirements. To address this challenge, a multifunctional CHm/PCA/Cu 2+ /ε-PL@Gel-OCS scaffold with enhanced mechanical properties, outstanding antibacterial, anti-inflammatory, pro-vascularization and osteogenic activities was developed via cryogenic 3D printing of a gelatin (Gel)/oxidized chondroitin sulfate (OCS) composite ink loaded with chitosan microspheres surface-functionalized by protocatechuic aldehyde (PCA), copper ions (Cu 2+ ) and ε-polylysine (ε-PL). Scanning electron microscopy and energy-dispersive X-ray analysis confirmed uniform dispersion of the microspheres and sustained release of therapeutic agents as the scaffold degraded. Rheological and mechanical testing demonstrated excellent print fidelity, interconnected porosity (161 ± 37 μm pores), and compressive strengths (100–200 MPa) suitable for cortical bone repair. Such porous structure and mechanical performance are highly compatible with human cortical bone microenvironment, which facilitates cell infiltration, nutrient exchange and mechanical load bearing. In vitro release studies revealed a sequential sustained release profile (OCS > Cu 2+ > ε-PL), ensuring a sustainable osteogenic, angiogenic, anti-inflammatory and antibacterial activity. The scaffold achieved 100% bactericidal efficiency against both Staphylococcus aureus and Escherichia coli , suppressed protein denaturation (anti-inflammation), and promoted neovascularization in a chick chorioallantoic membrane assay. Biocompatibility assays using MC3T3-E1 osteoblasts showed enhanced cell adhesion, proliferation, and live/dead viability over 5 days. Osteogenic potential was significantly elevated on the multifunctional scaffold, as evidenced by time-dependent increases in ALP activity, mineral deposition (Alizarin Red S), and upregulated expression of ALP, RUNX2, OPN, and OCN genes compared with Gel and Gel–OCS controls. Taken together, our cryogenic 3D-printed Gel-OCS scaffold incorporating PCA/Cu 2+ /ε-PL-functionalized chitosan microspheres provides a single-step, customizable platform that combines robust mechanical properties with multi-modal therapeutic functionalities. Different from conventional single-function bone scaffolds reported in most international studies, this multi-component synergistic design successfully realizes the integration of mechanical reinforcement, antibacterial, anti-inflammatory, vascularization and osteogenesis functions in one system. These promising preclinical results highlight a novel therapeutic strategy for bone defect regeneration, it solves the common bottlenecks of traditional gelatin-based bone scaffolds, provides a feasible and universal fabrication strategy for high-performance multifunctional bone repair materials, and offers new insights for the global development and clinical translation of 3D-printed bone tissue engineering scaffolds.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xiaodong Lin,Bergoi Ibarlucea,Tao Peng,Ren Shen,Peiyue Li,Pan Zhang
Susceptibility point-of-care testing (POCT) and bacterial inactivation are crucial for food safety and clinical diagnosis since bacteria pose a severe hazard to public health and healthcare. To enable photothermal determination and hyperthermal inactivation of bacteria, vancomycin-modified near-infrared (NIR)-responsive copper selenide nanoparticles (Cu 2−X [email protected] NPs) with excellent photothermal conversion are synthesized here. Cu 2−X [email protected] NPs can be trapped on the surface of Gram-positive bacteria such as Staphylococcus aureus ( S. aureus ), forming a Cu 2−X [email protected] NPs/ S. aureus complex. Suspension (Cu 2−X [email protected] NPs) and sediment (Cu 2−X [email protected] NPs/ S. aureus ) can convert the NIR laser energy into a thermal signal that can be detected by the thermal camera at concentrations as low as 6.0 CUF mL −1 . Meanwhile, S. aureus can be effectively inactivated by localized temperature rises during temperature-based signal detection. The antibacterial efficiency can reach as high as 99.53%. The proposed strategy not only provides a sensitive POCT strategy for bacterial determination but also implements efficient inactivation of bacteria simultaneously.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Lifen Wang,Yonggang Liu,Yinjun Lin,Yonghao Yu,Xueying Zhang,Ruiqin Zhang,Yunpu Zhai
In this study, an effective Ni-doped Cu 2 (OH) 3 F was synthesized by a simple one-step hydrothermal method to activate H 2 O 2 for degrading organic pollutants in water. The prepared (Cu 2−x Ni x ) 2 (OH) 3 F showed significant degradation performance for the degradation of RhB and TC. The characterization methods of XRD, XPS , TEM , SEM, FT-IR proved that Ni successfully replaced part of the Cu in Cu 2 (OH) 3 F site and provided more active sites for decomposition of H 2 O 2 through the synergistic effect of the bimetal . Besides, the photochemical properties of the prepared catalysts were analyzed by UV–vis, electrochemical impedance spectroscopy and transient photocurrent, which confirmed that the introduction of Ni narrowed the band gap of (Cu 2−x Ni x ) 2 (OH) 3 F and accelerated the separation of photogenerated electrons and hole pairs. Free radical capture experiments showed that •OH, h + , and •O 2 - all contributed to the reaction, and •OH was the main active substance. This research provides a photo-Fenton-like catalyst with simple synthesis, high performance and stable properties for future sewage treatment.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Immunology
·
2024
·
IF 5.7
Shen Man,Dai Xianling,Ning Dongni,Xu Hanqing,Zhou Yang,Chen Gangan,Ren Zhangyin,Chen Ming,Gao Mingxuan,Bao Jing
The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, H2O2 serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu2-xSe/GO nanostructure-based nanonzymatic H2O2 electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition. The obtained Cu2-xSe/GO@Pt/SPCE sensing electrode possesses a remarkable increase in specific surface derived from the three-dimensional surface constructed by GO nanosheets. Moreover, the localized surface plasma effect of the Cu2-xSe nanospheres enhances the separation of photogenerated electron-hole pairs between the interface of the Cu2-xSe NPs and the Pt NPs. This innovation enables near-infrared light-enhanced catalysis, significantly reducing the detection limit of the Cu2-xSe/GO@Pt/SPCE sensing electrode for H2O2 (from 1.45 μM to 0.53μM) under NIR light. Furthermore, this biosensor electrode enables in-situ real-time monitoring of H2O2 released by cells. The NIR-enhanced Cu2-xSe/GO@Pt/SPCE sensing electrode provide a simple-yet-effective method to achieve a detection of ROS (H2O2、-OH) with high sensitivity and efficiency. This innovation promises to revolutionize the field of wound inflammation treatment by providing clinicians with a powerful tool for accurate and rapid assessment of ROS levels, ultimately leading to improved patient outcomes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Fengping Zhan,Xiaolei Liao,Feng Gao,Weiwei Qiu,Qingxiang Wang
A novel electrochemical DNA biosensor has been facilely constructed by in-situ assembly of electroactive 4′-aminobenzo-18-crown-6-copper(II) complex (AbC-Cu 2+ ) on the free terminal of the hairpin-structured molecule beacon. The 3′-SH modified molecule beacon probe was first immobilized on the gold electrode (AuE) surface through self-assembly chemistry of Au–S bond. Then the crow ester of AbC was covalently coupled with 5′–COOH on the molecule beacon, and served as a platform to attach the Cu 2+ by coordination with ether bond (–O–) of the crown cycle. Thus, an electroactive molecule beacon-based biosensing interface was constructed. In comparison with conventional methods for preparation of electroactive molecule beacon, the approach presented in this work is much simpler, reagent- and labor-saving. Selectivity study shows that the in-situ fabricated electroactive molecule beacon remains excellent recognition ability of pristine molecule beacon probe to well differentiate various DNA fragments. The target DNA can be quantatively determined over the range from 0.10 pM to 0.50 nM. The detection limit of 0.060 pM was estimated based on signal-to-noise ratio of 3. When the biosensor was applied for the detection cauliflower mosaic virus 35s (CaMV 35s) in soybean extraction samples, satisfactory results are achieved. This work opens a new strategy for facilely fabricating electrochemical sensing interface, which also shows great potential in aptasensor and immurosensor fabrication.
✓ 已验证
DOI
PubMed
高影响因子
ACS ES&T Water
·
2025
·
IF 4.3
Xiaoming Zou,Yujin Li,Yanbo Zhou,Hong Chen,Xinrong Liu,Mengting Zhang,Tongtong Dong,Ligui Wu,Lingling Rong,Yuanmin Mo
In aquatic environments, microplastics (MPs), sulfonamides (SAs), and heavy metals frequently coexist due to their enduring persistence and continual influx. MPs exhibit a notable capacity for adsorbing both SAs and heavy metals, but their coadsorption behavior and the underlying mechanisms remain inadequately understood. This study explores the coadsorption behavior of ten SAs and copper ions (Cu2+) on polyamides (PA) in aqueous solutions. Results reveal that PA possesses an excellent adsorption capacity with synergistic effects for SAs and antagonistic effects for Cu2+. These effects persist under varying pH values, calcium ions, and humic acid. Mechanistic investigations indicate that these joint effects are not driven by the SA–Cu2+ complexation but by changes in PA’s surface properties, functional groups, and weak interactions. Introducing Cu2+ leads to variations in the functional groups at the interaction interface, resulting in an increase negative surface charge of PA. In coadsorption, interaction distances and energies for SAs and Cu2+ are significantly modified, particularly for electrostatic and van der Waals forces, due to enhanced electron transfer. Key SA quantum chemical descriptors (εα and π) correlate with these joint effects. These findings provide a new theoretical foundation for understanding coadsorption of pollutants on MPs.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）