SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Bin Jiang,Jiayun Guan,Yilin Lv,Chaojie Yang,Minfeng Yang,Xinyu Shen,Bin Xu,Qi Wang,Yuling Qin,Li Wu
The cholinergic hypothesis constitutes a pivotal theoretical framework for elucidating the pathological mechanisms underpinning Alzheimer's disease (AD). Quantitative assessment of cholinesterases (ChEs) and NAD(P)H levels in neuronal cells, particularly within the septo-hippocampal pathway, is imperative for the comprehensive study of the cholinergic system. In this study, we designed and synthesized a dual-channel, dual-enzyme-responsive fluorescent indicator that was chemically optimized for detecting levels of ChEs and NAD(P)H in neuronal cells. Biological experiments demonstrated that this indicator can sensitively and reliably visualize the dynamics of intracellular ChEs and NAD(P)H following stimulation. The corresponding ChEs activities and NAD(P)H concentrations demonstrated a linear correlation with fluorescence intensity, reaching equilibrium within a few minutes. This innovative and precise technique for visualizing ChEs and NAD(P)H dynamics with high spatial resolution during the progression of AD is expected to serve as a robust tool for investigating alterations in the cholinergic nervous system, including changes in acetylcholine neurotransmission and neuronal energy metabolism.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Guoqiang Zhao,Wenjing Chen,Rongrong Zhang,Rui Zhang,Bo Jiang,Ruyan Hou,Xing Huang
Cypermethrin, a common pyrethroid insecticide, raises significant ecological concerns due to its widespread use. In this study, a highly efficient cypermethrin-degrading strain, Rhodococcus sp. H-3, was isolated. The strain was capable of degrading 30 mg/L cypermethrin at the rate of 83.48 % within 7 days and it could hydrolyze cypermethrin into 3-phenoxybenzoic acid (3-PBA) and permethric acid (DCVA). A novel cypermethrin degradation gene, cymE1 , was identified in strain H-3 through genome analysis, and its highest level of amino acid sequence similarity observed was 33.50 % in the Swiss-Prot database. The functional strain, SCK6-E, which could secrete CymE1, was successfully constructed by using double-exchange strategies. Strain SCK6-E could be applied for the removal of cypermethrin residues from tea plant leaves with a removal rate of 57.83 % within 2 h. Furthermore, strain H-3 demonstrated the capability to remediate cypermethrin-contaminated soil, exhibiting a degradation rate of 65.80 % over 10 days. The results of the 16S rRNA high-throughput sequencing indicated that strain H-3 was conducive to the restoration of the diversity and richness of the bacterial community in the soil. This study provides novel strain and gene resources for researching the degradation mechanism of pyrethroids, and offers valuable insights for developing effective pesticide residue remediation strategies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2024
·
IF 4.3
Chunmiao Wang,Zhaoquan Li,Honglan Zhai,Xiaoyan Shen,Fengming Li,Qiuping Zhang,Danrong Li,Huaxin Hou
Background Cytoplasmic epidermal growth factor receptor (EGFR) is overexpressed in both nasopharyngeal carcinoma (NPC) and triple-negative breast cancer (TNBC), while clinical outcome and prognosis vary greatly among patients treated with gefitinib, and all patients eventually develop resistance to this agent. Therefore, we propose a new concept for synthesizing multitarget compounds and reveal new therapeutic strategies for NPC and TNBC expressing EGFR. Methods Compound H was synthesized in our previous study. Molecular docking, and cell thermal shift assays (CETSAs) and drug affinity responsive target stability(DARTS) were used to confirm the binding of compound H to EGFR and GLUT1. Methylthiazolyldiphenyl-tetrazolium bromide(MTT), annexin V-PE assays, mitochondrial membrane potential (MMP) assays, and animal models were used to evaluate the inhibitory effect of compound H on TNBC cell lines. Energy metabolism tests, Western blotting, and immunofluorescence staining were performed to evaluate the synergistic effects on EGFR- and glucose transporter type 1(GLUT1)-mediated energy metabolism. Results Compound H can simultaneously act on the EGFR tyrosine kinase ATP-binding site and inhibit GLUT1-mediated energy metabolism, resulting in reductions in ATP, MMP, intra-cellular lactic acid, and EGFR nuclear transfer. The anti-tumor activity of compound H is significantly superior to the combination of GLUT1 inhibitor BAY876 and EGFR inhibitor gefitinib. Compound H has remarkable anti-proliferative effects on TNBC MDA-MB231 cells, and importantly, no obvious toxicity effects of compound H were found in vivo . Conclusions Synergistic effects of inhibition of EGFR- and GLUT1-mediated energy metabolism by compound H may present a new strategy for the treatment of TNBC and NPC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2025
·
IF 6.3
Ziqian Ding,Xiaolin Qiu,Huaxuan Liu,Lingli Gu
Functional form-stable phase change materials (FPCMs) offer great promise for thermal management in transport packaging due to their stability after phase change cycle and the extension of service life. However, challenges remain in enhancing thermal conductivity and energy storage efficiencies. In this study, a magnetically aligned h-BN-embedded superporous carbon foam-based FPCM containing paraffin (M-BN-CPCMs) was fabricated via a foam polymerization method followed by high-temperature carbonization. A super-porous hydrogel (SPH) precursor was used to construct a robust, highly porous carbon skeleton to accommodate more PCMs. To further enhance thermal conductivity, h-BN was functionalized with Fe 3 O 4 nanoparticles, and the resulting BN-Fe 3 O 4 was magnetically oriented within the carbon skeleton using a synchronous magnetization process. This novel design achieved a thermal conductivity improvement of over 90% compared to SPH. Additionally, the synergistic photothermal conversion effect achieved by the magnetic nanoparticles and carbon skeleton resulted in an outstanding photothermal conversion efficiency of approximately 90% for M-BN-CPCMs. Notably, the incorporation of BN-Fe 3 O 4 increased pore sizes, enabling higher paraffin loading capacity. The M-BN-CPCMs with large holes exhibit a remarkable phase change enthalpy of 154.9 J/g, demonstrating excellent energy storage capability, which surpasses that of superporous carbon foam skeleton-based FPCMs (CPCMs). The optimized M-BN-CPCMs demonstrated superior thermal energy storage, faster thermal charging rates, and improved thermal regulation, effectively reducing temperature fluctuations within insulated packaging. These findings underscore the potential of M-BN-CPCMs as a groundbreaking solution for energy storage and thermal management in transport packaging, addressing critical challenges in the field.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ziying Guo,Yuting Zhou,Ping Wang,Qiang Wang,Bo Xu,Man Zhou,Li Cui,Ying Zhang,Yuanyuan Yu
The high energy consumption and pollution associated with traditional cotton fabric treatment have become increasingly serious concerns; thus, in this study, a synergistic cotton fabric treatment based on Novozymes pectinate lyase (NPL) scouring/ultraviolet light-mediated catalytic (UV/H 2 O 2 ) bleaching was innovatively proposed. This study investigated the degradation mechanisms of impurities in cotton fabrics, concluding that the oxidative bleaching reaction of morin follows first-order kinetics; in the UV/H 2 O 2 /NPL system, pectin degradation occurs through the β-elimination of NPL and oxidation by free radicals. Under optimized conditions—254 nm UV irradiation, 8 g⋅L −1 NPL, 30 g⋅L −1 H 2 O 2 , pH 9, and one-side irradiation for 45 min. The fabric whiteness could reach 79.04 %，but the fiber damage was severe, manifested by a 40 % decrease in polymerization and strength. After adjusting the process parameters, adjust the UV wavelength to 302 nm and add 6 g⋅L −1 of the activator (NOBS), whiteness of the fabric was about 76.7 % and strength retention rate was 96.38 %. This method overcomes the shortcomings of traditional pretreatment processes for cotton fabrics, under low temperature and alkaline conditions, low-strength damaged fabrics can be obtained, providing a new strategy for developing environmentally friendly pre-treatment processes for cotton fabrics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Yuwei Zeng,Chuang Huang,Dongming Duan,Aiju Lou,Yuan Guo,Tianhua Xiao,Jianguo Wei,Song Liu,Zhao Wang,Qihao Yang,Lei Zhou,Zenghui Wu,Le Wang
Insufficient vascularization is a major challenge in the repair of critical-sized bone defects. Deferoxamine (DFO) has been reported to play a potential role in promoting the formation of H-type blood vessels, a specialized vascular subtype with coupled angiogenesis and osteogenesis. However, whether DFO promotes the expression of H-type vessels in critical femoral defects with complete periosteal damage remains unknown. Moreover, stable drug loading systems need to be designed owing to the short half-life and high-dose toxic effects of DFO. In this study, we developed an injectable DFO-gelatin microspheres (GMs) hydrogel complex as a stable drug loading system for the treatment of critical femoral defects in rats. Our results showed that sustained release of DFO in critical femoral defects stimulated the generation of functional H-type vessels. The DFO-GMs hydrogel complex effectively promoted proliferation, formation, and migration of human umbilical vein endothelial cells in vitro. In vivo , the application of the DFO-GMs hydrogel complex expanded the distribution range and prolonged the expression time of H-type vessels in the defect area and was positively correlated with the number of osterix+ cells and new bone tissue. Topical application of the HIF-1α inhibitor PX-478 partially blocked the stimulation of H-type vessels by DFO, whereas the osteogenic potential of the latter was also weakened. Our results extended the local application of DFO and provided a theoretical basis for targeting H-type vessels to treat large femoral defects. Statement of significance Abundant functional blood vessels are essential for bone repair . The H-type blood vessel is a functional subtype with angiogenesis and osteogenesis coupling potential. A drug loading system with long-term controlled release was first used to investigate the formation of H-type blood vessels in critical femoral defects and promotion of bone repair. Our results showed that the application of DFO-GMs hydrogel complex expanded the distribution range and expression time of H-type vessels, and was positively correlated with the number of osteoblasts and volume of new bone tissue. These results expanded the local application approach of DFO and provide a theoretical basis for targeting H-type vessels to treat large femoral defects.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 31
·5968-5975
·
IF 7.571
Yanhui Guo,Shuheng Huang,Han Sun,Zhe Wang,Yutong Shao,Lukun Li,Zhiliang Li,Fengling Song
Due to their excellent electronic and optical properties, porphyrins are extensively studied conjugated macrocycles in supramolecular chemistry for assembling functional nanomaterials. Although the aggregation of monomers plays a significant role in driving the self-assembly process into ordered nanostructures, it remains a challenge for tuning the self-assembling behavior of porphyrins through molecular structure modifications, especially in aqueous solutions. In the present work, two novel water-soluble porphyrin derivatives were synthesized by introducing cationic linear side chains into the π-conjugated core for phosphate-templated assembly through electrostatic interactions. It was found that the stacking patterns (H- or J-type aggregation) of porphyrins could be tuned by varying the number of side chains, which are associated with dramatic morphological change. The cytotoxicity and photodynamic properties of the J-aggregation-driven nano-assemblies were also investigated for the purpose of anti-cancer treatment. This study demonstrates a facile and effective strategy to regulate the aqueous self-assembling behavior of porphyrins that can impact the structure and properties of assembly, which will be of great benefit to the design and synthesis of functional nanomaterials for specific applications.
研究领域:
超分子化学
·
纳米材料
·
生物医学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
TETRAHEDRON
·
2019
·Vol. 75
·期 31
·4174-4179
·
IF 2.379
Yingying Zuo,Feng Xiong,Jing Zhao
Organic sulfur compounds have broad applications in biology, medicine and material sciences and intensive efforts have been devoted to developing mild and general C–S bond-forming methods. However, a mild, transition-metal-free, direct C–H bond functionalization method remains elusive. Here, we report the use of an internal oxidant-directing strategy to achieve this goal. The cascade reactions described here show excellent chemoselectivity and a wide substrate scope for both oxyamines and sulfenylation reagents. This study enlarges the synthesis toolbox for preparing structurally diverse sulfilimines under mild conditions.
研究领域:
有机化学
·
合成化学
·
药物化学
·
生物医学材料
·
分子生物学
✓ 已验证
DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2025
·
IF 7
Kexin Xu,Yifan Lv,Xinrui Cui,Wenyu Lai,Cun-Yue Guo,Baolin Li
As a type of organic small molecule (OSMs), the introduction of sulfur atoms in fused-ring thiophenes can reduce the dihedral angle between fused rings, which helps to form planar structures and makes it easier to form tightly packed crystal structures, creating more continuous paths for charge transfer. Therefore, they have aroused widespread research interest. However, little research has been conducted on benzo[1,2-b:3,4-b′:5,6-b″]trithiophenes (BTTs), and their simple and efficient syntheses still need to be urgently developed. Herein, we have developed a synthetic approach for BTTs via C–H thienannulation. This protocol features streamlined domino synthetic procedures, eliminating the requirement for the introduction of halogen functional groups, catalysts, and additives. BTTs exhibit planar structures, enabling tight crystal packing that creates a continuous pathway for charge transport. These structural attributes endow BTTs with substantial promise for application in organic electronic materials. Therefore, 2,5,8-tris(4-propylphenyl)benzo[1,2-b:3,4-b′:5,6-b″]trithiophene ((C3-Ph)3-BTT) and its brominated derivative ((C3-Ph)3-Br3-BTT) were investigated as organic small molecules (OSMs) for doping with single-walled carbon nanotubes (SWCNTs) to fabricate OSM/SWCNT composite thermoelectric materials. The composite films exhibited excellent thermoelectric performance, achieving a maximum power factor of 515.23 ± 3.54 μW m–1 K–2 at room temperature. This value represents the highest reported power factor for thiophene derivative/SWCNT thermoelectric composites, marking a significant advancement in the development of high-performance composite thermoelectric materials based on thiophene derivatives.
✓ 已验证
DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2017
·
IF 4.849
Xiaoxia Wang,Na Li,Zhongfeng Li,Honghua Rao
A dehydrogenative C(sp2)–N bond-forming strategy via copper-catalyzed intramolecular C–H/N–H coupling has been developed, which systematically unraveled the feasibility and practicality for benzoimdazo[1,2-a]indole formations through oxidative anilidic N–H activation. The merit of this strategy is illustrated by the broad tolerance of functionalities, as well as the utilization of extremely cheap copper catalysis to realize potentially useful indole-fused tetracycles in a step- and atom-economical manner.
研究领域:
有机化学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2022
·
IF 5.723
Shipei He,Sheng Fan,Kai Yuan,Shujia Guo,Sen Wang,Zhangfeng Qin,Mei Dong,Weibin Fan,Jianguo Wang
Herein, K + was added in the synthesis gel to regulate the distribution of acid sites in the ITQ-13 zeolite and enhance its catalytic performance in the conversion of methanol to olefins (MTO). The results indicate that a proper content of K + ions in the synthesis gel can not only expedite the crystallization of ITQ-13 zeolite, but also significantly decrease the proportion of paired Al and distribute more Al atoms in the 10-ring sinusoidal channel out from the intersection cavity. Accordingly, over the H-ITQ-13(0.3 K) zeolite prepared using the synthesis gel with a KCl/Al 2 O 3 molar ratio of 0.3, the alkene cycle of MTO keeping to the dual cycle hydrocarbon pool (HCP) mechanism is preferably enhanced, whereas the hydride transfer and aromatization reactions are substantially suppressed. As a result, H-ITQ-13(0.3 K) exhibits much longer catalytic lifetime and higher selectivity to propene and butene , in comparison with the H-ITQ-13 counterpart obtained without adding K + .
研究领域:
化学工程
·
催化材料科学
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Yangqing He,Na Xu,Lucas Binnah Junior,Xing Hao,Binghua Yao,Qian Yang,Dongjie Liu,Zhanying Ma
Construction of semiconductor-assisted noble metal nanocomposites has been approved as an efficient strategy to achieve promising photocatalysts. In this paper, highly efficient AuNPs/h-BN nanocomposites have been fabricated by loading gold nanoparticles (AuNPs) as interfacial electron transfer mediators onto the hexagonal boron nitride nanosheets (h-BN). The SEM and HRTEM images, together with XPS analysis revealed that AuNPs were successfully deposited onto the surface of h-BN and the layered heterojunctions were generated between AuNPs and h-BN. The optimum 15%AuNPs/h-BN nanocomposites exhibit dramatically enhanced photocatalytic performance for degradation of levofloxacin hydrochloride with 84.4% removal rate, and hydrogen evolution of 61.15 μmol within 5 h. It is almost 4 and 61 times higher than that of pure h-BN, respectively. In addition, this is the first time using h-BN based material to evaluate the elimination efficiency of levofloxacin hydrochloride. The corresponding enhanced mechanism for 15%AuNPs/h-BN can be attributed to the effective interfacial electron-hole pairs separation and migration between h-BH and AuNPs and its surface Plasmon absorption (SPA). Moreover, the possible decomposition pathways for levofloxacin hydrochloride are proposed according to the UPLC-MS analysis. Herein, we have provided a good example for removing antibiotic residues by using h-BN-based metal nanocomposites towards environmental remediation.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）