JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Wang Suyu,Zabed Hossain M.,Zhang Guoyan,Yun Junhua,Tian Yuehui,Zhang Yufei,Qi Xianghui
Ergothioneine (EGT) is a high-value antioxidant for food,
pharmaceutical,
nutraceutical, and cosmetic applications. Microbial production from
renewable feedstocks is promising, but efficient biosynthesis requires
coordinated precursor supply. Precursor-supplementation experiments
indicated that multiple amino acids may jointly influence EGT production.
Based on pathway biochemistry and previous engineering evidence, endogenous l -cysteine supply was selected as one mechanistically relevant
engineering target. Here, an Escherichia coli platform for glucose-derived EGT production was developed by combining
biosensor-driven evolution and transcriptome-guided chassis engineering.
A dual-output l -cysteine-responsive biosensor linked kanamycin
resistance and fluorescence, enabling sequential growth-based enrichment
and fluorescence-based prioritization. Mutagenesis, microdroplet-assisted
adaptive evolution, and single-cell sorting generated an evolved chassis
with 47.10% higher extracellular l -cysteine accumulation
and 64.49% higher EGT production. Transcriptome-guided bsmA activation further improved production, and the final strain produced
1.20 g/L EGT in a 3 L fed-batch fermentation without additional l -cysteine supplementation.
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高影响因子
Food Bioscience
·
2026
·
IF 6.2
Dongling Yan,Mingming Zheng,Kaige Li,Huanyu Zheng,Yufei Zhang
L-α-Glycerylphosphorylcholine (GPC) is a key biosynthetic precursor of the human neurotransmitter acetylcholine with multiple biological functions. Currently, the enzymatic preparation of GPC is limited by the poor stability, low reusability and unsatisfactory catalytic efficiency of free phospholipase A 1 (PLA1). To address these limitations, this study constructed aqueous catalytic system using anion-exchange acrylic resin LXTE-902 immobilized PLA1 as the biocatalyst and egg yolk lecithin (PC) as the substrate for GPC preparation. The results indicated that LXTE-902 exhibited excellent immobilization performance for PLA1, with an immobilization capacity of 65.9 mg/g. Under optimized conditions, the immobilized PLA1 efficiently catalyzed PC hydrolysis within 1 h, achieving a high GPC yield of 93.7%. A facile solvent extraction and precipitation purification method was further developed to efficiently separate free fatty acids from the reaction system, affording high-purity GPC with a purity of 92%. Compared with free PLA1, the immobilized biocatalyst possessed superior storage stability, pH tolerance and solvent tolerance, and retained a GPC yield of 40% after seven reuse cycles. This work demonstrates that LXTE-902-immobilized PLA1 is an efficient, stable and recyclable biocatalyst. The proposed enzymatic strategy provides a promising technical reference for industrial-scale production of high-purity GPC.
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高影响因子
BMEMat
·
2026
·
IF 17.2
Pai Peng,Dongchao Qiu,Yongyuan Kang,Qiaoxuan Wang,Xuelong Wang,Boyana Parvanova,Miroslav Karabaliev,Changyou Gao
Bioprosthetic heart valves (BHV) remain limited by progressive calcification, infection, and structural degeneration, leading to premature failure despite decades of clinical use. Herein, a rapid and efficient surface grafting strategy based on hyperbranched poly-L-lysine (HBPL) was developed to re-engineer the pericardial interface toward improved functional performance and translational potential. The HBPL-surface engineering enhanced the structural stability, resistance to enzymatic degradation, and anti-calcification performance of bovine pericardium (BP). Mechanistic investigations revealed that the hyperbranched topology and dense amino functionality of HBPL regulate calcium binding behavior and inhibit the nucleation and phase transformation of amorphous calcium phosphate. In addition, HBPL modification significantly reduced bacterial adhesion while maintaining favorable cytocompatibility and hemocompatibility. Arteriovenous shunt (AV-shunt) assays ex vivo demonstrated improved blood compatibility under flow conditions, and subcutaneous implantation in rats showed attenuated inflammatory responses and reduced calcification. Furthermore, balloon-expandable valves fabricated from HB-BP exhibited satisfactory hydrodynamic performance and durability exceeding 200 million fatigue cycles. Collectively, these results identify the HBPL-based surface engineering as a simple and versatile platform for developing BHVs with enhanced resistance to calcification and infection, improved durability, and promising translational potential.
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高影响因子
Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Jianjun Kang,Xue Lin,Chen Lin,Wuyuan Pan,Jian Wang,Fang Ke
Levodopa (L-DOPA) is a crucial neurotransmitter utilized in the treatment of neurological disorders, particularly in Parkinson's disease, where its concentration in biological fluids serves as a key diagnostic marker. Despite its significance, current methods for L-DOPA detection often suffer from limitations such as prolonged detection times, low sensitivity and weak anti-interference. This study addresses these challenges by introducing a highly efficient and colorimetric sensor for the rapid detection of L-DOPA, utilizing manganese-doped graphitic carbon nitride (Mn-g-C 3 N 4 ) as a peroxidase-mimetic catalyst. The sensor employs 3,3′,5,5′-tetramethylbenzidine as a substrate and achieves detection within 20 s, with a linear response over the concentration ranges of 1–10 μM and 20–90 μM, and the detection limits were 0.049 μM and 0.51 μM, respectively. The method has good specificity for L-DOPA and has been successfully applied to human serum even in the presence of interference from dopamine and other amino acids. In addition, the sensor exhibits satisfactory performance in smartphone-based L-DOPA detection. The novel sensor platform offers a simple, cost-effective, and scalable solution for real-time L-DOPA monitoring, with significant potential for applications in both clinical and environmental contexts.
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高影响因子
Food Bioscience
·
2025
·
IF 5.9
Li Xiaolin,Wu Zhen,Luo Liyong,Zeng liang,Wang Yuanhui,Wang Yongde,Luo Wei
This study investigates the protective effects of bergamot (Citrus medica L. var. sarcodactylis Swingle) extract on acute alcoholic gastric ulcers in mice model. The extract was analyzed for its chemical composition, total phenol and flavonoid contents, and antioxidant activities using DPPH and ABTS assays. Acute gastric ulcers were induced in mice with anhydrous ethanol, and the protective effects of the extract were evaluated by assessing gastric tissue morphology, serum superoxide dismutase (SOD), malondialdehyde (MDA), and inflammatory markers (TNF-α, IL-6, and IL-1β). LC-MS analysis revealed 674 components in the extract, including flavonoids, saponins, and other bioactive compounds. Quantification analysis of individual phenolic compounds such as gallic acid, ferulic acid, p-coumaric acid, hesperidin, rutin, neohesperidin, naringin, eriocitrin, limettin, narirutin, oxypeucedanin, and narcissoside showed a total amount of 1451 mg/kg. The total phenol content was 4.74±0.24% DW (dry weight), and the total flavonoid content was 0.64±0.04% DW. The extract showed good antioxidant activity, with DPPH and ABTS IC 50 values of 287.65±5.38 μg mL -1 and 326.31±10.89 μg mL -1 respectively. Treatment with bergamot extract significantly decreased the gastric ulcer index, increased the ulcer inhibition rate, reduced MDA levels, and increased SOD activity, improving gastric mucosal injury. Furthermore, inflammatory markers TNF-α, IL-6, and IL-1β were significantly reduced ( p < 0.01). These findings suggest that bergamot extract may offer effective protection against alcohol-induced gastric injury.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Shuo Shi,Xi Lan,Jianxing Feng,Yongsheng Ni,Mingqiang Zhu,JingSun,Jianlong Wang
Designing wound dressing materials with hemocompatibility, suitable mechanical properties, outstanding hemostatic effects and anti-inflammatory activity is of great practical significance for wound management. Herein, a hemostatic hydrogel loaded with Lycium barbarum L. polysaccharide (LBP)-functionalized ultrathin MMT nanosheets (L-MMT NSs) was fabricated for efficient hemostasis and wound healing . Loading the L-MMT NSs into polyvinyl alcohol (PVA), the obtained P-L-MMT hydrogel exhibited a 3D porous structure with good swelling properties, cytocompatibility, hemocompatibility, and anti-inflammatory activity. Importantly, in vivo investigations demonstrated that the P-L-MMT hydrogel exerts outstanding hemostasis activity in the hemorrhaging mouse liver model and reduces tissue damage caused by inflammation to shorten wound healing time. Altogether, the convenient exfoliation and functionalization of bulk MMT using LBPs make this inexpensive and rising nanostructure more attractive in the application of nanomedicine. Moreover, due to the synergy between hemostasis and anti-inflammation, this newly developed multifunctional P-L-MMT hydrogel represents a promising material in biomedical fields.
研究领域:
生物材料学
·
医学
·
纳米医学
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高影响因子
Journal of Materials Chemistry A
·
2026
·
IF 9.5
Bing Liu,Haoming Zhong,Hina Gul,Hao Peng,Xiaolei Guo,Yu-Long Xie,Minhua Ai,Xiangwen Zhang,Ji-Jun Zou,Lun Pan
Biomass electrooxidation for coupled hydrogen production offers a promising route to overcome the sluggish kinetics and high energy cost of conventional water electrolysis. The electrocatalytic oxidation of L-ascorbic acid (AA) to high-value dehydroascorbic acid (DHA) is particularly attractive but requires efficient catalysts to facilitate the challenging two-step dehydrogenation process. Here, we report a FeRu-NC catalyst with excellent L-ascorbic acid oxidation reaction (AAOR) catalytic activity and selectivity. The FeRu-NC catalyst achieves a significantly lower overpotential of 10 mV at 10 mA•cm⁻² and delivers a high current density of 200 mA•cm⁻² at only 0.68 V vs. RHE, while maintaining stable operation for over 80 h. DHA is produced with high yield (~95%), selectivity (~97%), and Faradaic efficiency (~95%). XPS, XAFS studies and in-situ IR spectroscopy reveal that the introduction of trace Ru modulates the electronic structure of Fe single-atom sites, enhancing the adsorption of key intermediates and promoting the second dehydrogenation step, presenting a highperformance AAOR activity and high selectivity for DHA.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Guangai Sun,Shijie Guo,Yuanyuan Yao,Zeyu Lin,Hui Gao,Roland H. Stauber,Bin-Chun Li,Guo-Bin Ding
α-L-Rhamnosidase is a significant biocatalyst that specifically cleaves terminal α-L-rhamnose group from natural flavonoid diglycosides, enabling the biocatalytic production of high-value flavonoid glucosides like prunin from naringin. Compared to its precursor naringin, prunin exhibits superior solubility and bioavailability, making this enzymatic conversion commercially valuable for the food and pharmaceutical industry. However, the industrial application of free α-L-rhamnosidases faces significant challenges, including enzyme instability, difficulty in recovery, and unsatisfactory reusability. To address these issues, this study developed an innovative magnetically recoverable biocatalyst (Fe₃O₄-Rha) through covalent immobilization of recombinant α-L-rhamnosidase from human fecal metagenome onto Fe₃O₄ nanoparticles using EDC/NHS chemistry, and the immobilization parameters were systematically optimized. The successful preparation of Fe₃O₄-Rha was verified by TEM, FTIR, TGA, and SQUID analysis. Fe₃O₄-Rha retained the catalytic property of free Rha in terms of optimal pH and temperature but exhibited superior tolerance on organic solvent especially ethanol and isopropanol. Moreover, Fe₃O₄-Rha could effectively biotransform naringin to prunin and maintained 61.34% of initial activity after 5 cycles. In a scaled-up reaction system, Fe₃O₄-Rha also efficiently converted naringin into prunin and the complete conversion was achieved within 10 h. This work successfully developed a magnetically recoverable immobilized α-L-rhamnosidase system for the efficient and reusable biotransformation of naringin, offering a promising approach for the enzymatic modification of bioactive small molecules using biological macromolecular catalysts.
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PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Hongtao Du,Juncheng Chen,Shan Tian,Hongling Gu,Na Li,Yao Sun,Jiajia Ru,Junru Wang
In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82 ± 0.38%, which is in good agreement with the predicted value (9.93 ± 0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70 kDa and 4.39 kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400 μg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods .
研究领域:
植物化学
·
免疫学
·
食品科学
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高影响因子
Natural Product Communications
·
2025
·
IF 1.4
Phuong Nhung Nguyen,Ngoc Lien Nguyen,Hai Nam Pham,Phuong Khanh Nguyen,Kieu Oanh Nguyen Thi
ObjectivesPteris vittata L. is one of the hyperaccumulator plants that can grow in heavy metal-contaminated soil. This plant may tolerate metal stress through different mechanisms, eg, by producing a wide range of secondary metabolites. These compounds play an essential role in physiological function and can also serve as rich sources of chemicals for health benefit purposes. This study aims to determine the phytochemicals of plant extracts and the relationship between these profiles with pharmacological properties to predict active agents and their interaction.MethodsThe total phenolic and total flavonoid contents were measured by colorimetric assays, while the widely targeted metabolomics was applied to reveal leaf and root P. vittata metabolome. In parallel, the antioxidant and antibacterial effects of these extracts were determined by ABTS scavenging and micro-broth dilution methods. Multivariate analysis, ie, Partial Least Square Regression, calculated the correlation between metabolites and bioactivities.ResultsThe results showed the significant content of phenolics and flavonoids in plants collected from mining soils of Thai Nguyen province, Vietnam, and their potential ABTS scavenging activity. Additionally, the plant's metabolome characterized 82 compounds, mainly secondary metabolites. The multivariate analysis indicated the positive correlation among quercetin derivatives and bioactivity, suggesting the contribution of their combination, not individually, to the antibacterial effect of P. vittata.ConclusionHyperaccumulators in metalliferous soils are potential reservoirs for exploring the antibacterial agents against multi-drug resistant strains, and this effect may be exerted by additive or synergistic mechanisms of action of multiple compounds.
研究领域:
植物学
·
药理学
·
环境科学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 99
·81482-81491
·
IF 3.84
Xuefei Leng,Zhiyong Wei,Yingying Ren,Yang Li,Yurong Wang,Qinyi Wang
In this work, two series of linear-comb and star-comb well defined graft poly(L-lactide) (PLLA) have been synthesized conveniently by one-pot ring-opening polymerization (ROP) of L-lactide using functionalized polybutadiene macroinitiators. The used organocatalyst of 1,8-diazabicyclo[5,4,0]undec-7-ene (DBU) allows the polymerization of L-lactide to proceed rapidly at room temperature. Kinetic studies of the ROP reaction in this system indicate first-order kinetics in monomer concentration. 1H NMR and GPC techniques are employed to characterize the synthesized polymers, validating the formation of the desired comb structures with controllable chain length. Linear-comb and star-comb graft PLLA were comparatively studied as well as with linear PLLA by DSC and POM. The results reveal that the comb structure gives a remarkable improvement of PLLA crystallization ability in both crystallinity and growth rate of spherulites. Furthermore, the more compacted star-comb structure imposes restriction on chain mobility, which weakens the growth effect to some extent. It is found that the glass transition temperature (Tg) and melting temperature (Tm) significantly depend on the side chain length and backbone structure. Rheological studies of both melt and instinct viscosity of the solution show that star-comb PLLA has the lowest hydrodynamic volume compared with linear-comb PLLA and linear PLLA.
研究领域:
高分子化学
·
材料科学
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高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2014
·Vol. 50
·期 11
·1065-1071
·
IF 0.66
Chao Mingyong,Ma Xinying
Herein, a poly( L -tryptophan) modified glassy carbon electrode (Ptry/GCE) for the determination of maltol is fabricated by electrochemical polymerisation. The electrochemical behaviour of maltol at the Ptry/GCE is studied by cyclic voltammetry (CV). The modified electrode shows excellent electrocatalytic activity towards the oxidation of maltol and the oxidation is a one-proton-one-electron process. In pH 8.0 phosphate buffer solution (PBS), the oxidation peak current of maltol shows a linear relationship with its concentration in the range from 9.00 × 10 −5 to 3.75 × 10 −3 mol L −1 with a correlation coefficient of 0.9972. The limit of detection is estimated to be 8.00 × 10 −6 mol L −1 . The novel method shows good selectivity, recovery, reproducibility and great convenience and has been satisfactorily demonstrated in real food sample analysis.
研究领域:
电分析化学
·
食品安全
·
传感器与生物传感器
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高影响因子
危险品化学品经营许可证（带存储）