Biomass Conversion and Biorefinery
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2021
·Vol. 13
·期 8
·7175-7184
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IF 4.987
Yang Chunyan,Zhang Liu,Zhu Yingming,Liu Yingying,Liu Changjun,Wu Kejing,Lu Houfang,Liang Bin
In this study, ordered mesoporous carbon materials are prepared to support β-Mo 2 C catalysts for conversion of Kraft lignin into aromatic monomers. Carbon supports with different porous structures are compared to discuss the critical role of ordered mesopores. The results show a volcano evolution of aromatic monomer yields with the increase in Mo contents over β-Mo 2 C supported on ordered mesoporous carbon, and a high aromatic yield of 0.576 g/g Kraft lignin is achieved at 280 °C for 3 h for only 10% Mo mass content. Dispersion of β-Mo 2 C and confinement of ordered mesopores contribute to the highest aromatic yield per unit Mo. The highly dispersed β-Mo 2 C on the surface of ordered mesoporous carbon exhibits the advances of confined environment and accessibility of lignin fragments to active β-Mo 2 C, resulting high activity of β-Mo 2 C for Kraft lignin decomposition.
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Food Science & Nutrition
·
2021
·Vol. 9
·期 3
·1399-1409
·
IF 2.863
Mengna Cao,Jian Gao,Yang Li,Chengzhi Liu,Jieyu Shi,Fangfang Ni,Gerui Ren,Hujun Xie
Heat treatment is widely used in food industry. Proteins and polysaccharides as important natural polymers in food, under heat treatment, the interactions between them could mediate the conformation and functional properties of proteins. Thermally induced β-lactoglobulin-gum arabic complexes (β-Lg-GA) were fabricated, and the effect of heat treatment on physicochemical properties of the complexes was systematically investigated. The average particle size of β-Lg-GA complexes decreased with temperature increased, at 85°C, a smaller size of 273 nm was obtained. A saturated adsorption of GA was found when mass ratio of β-Lg/GA was <1:2. At pH = 4.2–7.0, electrostatic attraction between β-Lg and GA was low and a fairly constant turbidity was observed, the formed composite particles had good stability to the pH value. Through UV, fluorescence, and FTIR spectroscopy, it was found that formation of the nanoparticles relied on thermal denaturation and aggregation of protein, the electrostatic, hydrophobic, and hydrogen bonding interactions between β-Lg and GA were also important. Scanning electron microscope further indicated β-Lg and GA had good compatibility, and the complexes had a spherical core–shell structure at molecular level. In addition, these prepared natural nanoparticles by heat treatment show significantly higher encapsulation efficiency for (-)-epigallocatechin-3-gallate (EGCG) than that of unheated, thus could be used as a promising carrier for biologically active substances.
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Journal of Materials Chemistry B
·
2020
·Vol. 9
·期 1
·170-175
·
IF 5.344
Fang Fan,Li Zhang,Xinguang Zhou,Fangya Mu,Guoyue Shi
The development of non-invasive and sensitive optical probes for in vivo bioimaging of cancer-related enzymes is desirable for early diagnosis and effective cancer therapy. β-galactosidase (β-gal) is regarded as a key ovarian cancer biomarker, owing to its overexpression in primary ovarian cancer. Herein, we designed a sensitive near-infrared (NIR) probe (DCMCA-βgal) for the detection and real-time imaging of β-gal activity in ovarian tumors, thereby achieving the visualization of ovarian tumors by β-gal activity detection. DCMCA-β-gal could be triggered by β-gal, resulting in the release of a NIR chromophore, DCM-NH2; the linear range of fluorescent response to β-gal concentration was 0–1.2 U with a low detection limit of 1.26 × 10−3 U mL−1. We used DCMCA-β-gal to detect and visualize β-gal activity in SKOV3 human ovarian cancer cells, as well as for real-time imaging of β-gal activity in ovarian cancer mouse models. DCMCA-β-gal possessed high sensitivity, “turn-on” NIR emission, a large spectral shift, and high photostability in a dynamic living system and thus could serve as a highly sensitive sensor for real-time tracking of β-gal activity in vivo and ovarian tumor imaging.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Chaoying Qiu,Mengting Lei,Wan Jun Lee,Ning Zhang,Yong Wang
Oleofoams have emerged as attractive low-calorie aeration systems, but saturated lipids or large amount of surfactants are commonly required. Herein, an innovative strategy was proposed to create oleofoams using medium-long chain diacylglycerol (MLCD) and β-sitosterol (St). The oleofoams prepared using MLCD and St in ratios of 15:5 and 12:8 exhibited smaller bubble size and much higher stability. MLCD crystals formed rigid Pickering shell, whereby air bubbles acted as “active fillers” leading to enhanced rigidity. Both Pickering and network stabilization for the MLCD-St oleofoam provided a steric hindrance against coalescence. The gelators interacted via hydrogen bonding, causing a condensing effect in improving the gel elasticity. The oleofoams and foam-based emulsions exhibited a favorable capacity in controlling volatile release where the maximum headspace concentrations and partition coefficients showed a significantly decrease. Overall, the oleofoams have shown great potential for development of low-calorie foods and delivery systems with enhanced textural and nutritional features.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Jia Li,Yuankun Wang,Zhifeng Wang,Jun Wang,Defeng Wu
Chitin nanocrystal (ChNC) is good nucleation agent for aliphatic polyesters because of its high-energy surface. To moderate its nucleation activity, silane coupling agents with different chain lengths or functional groups were used to modify ChNCs in this work, and biodegradable poly( β -hydroxybutyrate) (PHB) was used as target polymer for crystallization study. Surface coupling of ChNCs improves their phase adhesion to PHB chain and weakens their nucleation activities. The alterations strongly depend on the surface chain structure of ChNCs: sulfhydryl silane-coupled ChNC shows lowered nucleation activity, whereas amino silane-coupled ChNCs even become antinucleation agents. The interfacial compatibility is vital to altered role of ChNCs and to following changes in spherulite growth and ring-banded morphology, which is further disclosed using Flory-Huggins interaction parameters and rheological responses as probes. This work provides useful information on tailoring the functions of ChNCs as nanoadditive for biodegradable aliphatic polyesters by the way of surface chain engineering.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 2
·1-8
·
IF 5.833
Yin Lixiu,Wang Yueju,Tan Rong,Li Huiling,Tu Yifeng
In order to effectively and conveniently detect the β-amyloid oligomer (AβO) for earlier diagnosis of Alzheimer’s disease (AD), a disposable aptamer biosensor has been developed with high performance, facile operation, and low cost. Using a nanocomposite by in situ reduction of chloroauric acid to decorate Au nanoparticles (AuNPs) on Fe-MIL-88NH 2 material via Au–N bond to effectively enhance the electrochemiluminescence (ECL) of luminol, the functioned basal electrode provides adequate background for sensing response. When the aptamer is linked via Au–S bond on the surface, the sensor gets the ability of specific recognition and coalescence toward the target (AβO). After incubating the sample on the aptasensor, its ECL signal is inhibited owing to the steric hindrance of the AβO macromolecules. The relative inhibition ratio linearly depends to the logarithm of AβO concentration in the range 0.1 pM to 10 pM, with an LOD of 71 fM. The aptasensor has high selectivity to AβO among its analogs. The recoveries in human serum were 98.9–105.4%. This research provides a new approach for sensitive detection of AβO in clinic laboratories for investigation and diagnosis of AD. Graphical abstract
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COLLOID AND POLYMER SCIENCE
·
2020
·Vol. 298
·期 3
·303-312
·
IF 1.536
Gao Feng,Wang Qiang,Yang Xiaochun
Synthesis of amphiphilic poly( β -amino esters) (PBAE) was optimized by stepwise feeding of monomers (PBAE-(P-H)) compared with one-pot synthesis (PBAE-(P&H)) based on poly(ethylene glycol) diacrylate (PEGDA) and 1,6-hexanediol diacrylate (HDDA). Interestingly, optimized synthetic PBAE-(P-H) had smaller critical micelle concentration (CMC) of 6.6 μg/mL on average than one-pot synthetic PBAE-(P&H) (10.4 μg/mL). Nanoparticles were prepared based on PBAE-(P-H) and PBAE-(P&H), respectively. PBAE-(P-H) nanoparticles exhibited more narrow size distribution (average size, 105.3 nm; PDI, 0.103) than PBAE-(P&H) nanoparticles (average size, 117.8 nm; PDI, 0.233) demonstrated by dynamic light scattering and transmission electron microscopy. Afterward, PBAE-(P-H) and PBAE-(P&H) nanoparticles were selected to further investigate the properties including charge conversion and pH responsiveness. Doxorubicin (DOX) was encapsulated into nanoparticles. DOX release in vitro was enhanced as the decrease of pH value. Furthermore, DOX-loaded PBAE-(P-H) and PBAE-(P&H) nanoparticles exhibited enhanced cytotoxicity and cellular uptake against HCT116 cells compared with DOX under tumor microenvironment.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2020
·Vol. 31
·期 6
·4797-4807
·
IF 2.22
Lyu Cong,Ju Lunan,Yang Xuejiao,Song Lan,Liu Ning
Iron-based catalysts as peroxymonosulfate (PMS) activator are commonly used to oxidize and degrade azo dye. However, there are still some issues such as low catalytic performance, relatively less reactive oxygen species and high metal iron dissolution. As such, a novel iron oxyhydroxide (FeOOHs) with four crystal form was synthesized and employed as efficient PMS activator for AO7 degradation in this study. It was found that the crystal form of catalyst had significant influence on its catalytic performance. β -FeOOH exhibited the best catalytic performance, owing to its smallest lattice size, maximum specific surface area and highest surface hydroxyl density. β -FeOOH/PMS system possessed a great high apparent reaction rate constant (1.3919 h −1 ), which was about 17, 3, 31, 20, 14, 49 and 23 times higher than that in β -FeOOH/persulfate(PS), Fe 0 /PMS, Fe 0 /PS, Fe 2 O 3 /PMS, Fe 2 O 3 /PS, Fe 3 O 4 /PMS and Fe 3 O 4 /PS system, respectively. More importantly, radical quenching tests and Electron spin resonance (ESR) analysis revealed that OH . , SO 4 .− , O 2 .− and 1 O 2 were involved in the reaction process. The catalytic mechanism proposed that β -FeOOH had the effective electron transfer rate performance, and Fe 3+ underwent a reduction to Fe 2+ during the β -FeOOH/PMS catalytic oxidation process. Then, the regeneration of Fe 2+ on the hydroxylated surface of FeOOH promoted the formation of FeOH + , which was crucial for PMS activation and reactive oxygen species (ROS) generation. The recyclability tests proved good stability and reusability of β -FeOOH. This study provides a kind of new understanding of peroxymonosulfate activation for environmental remediation and advances the study of iron-based oxyhydroxide in heterogeneous catalysis.
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FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Yao Hu,Yang Qin,Chao Qiu,Xueming Xu,Zhengyu Jin,Jinpeng Wang
In this study, a starch-based nanocarrier was fabricated to allow tangeretin to avoid the disadvantages of poor aqueous solubility and weak stability. A green technique comprised of a combination of ultrasonication and recrystallization was applied, which ensured the products would be more applicable in the food , pharmaceutical, and cosmetic industries. Debranched starch (DBS) and β-cyclodextrin (β-CD) underwent ultrasonic irradiation and were self-assembled at 4 °C to form CD/DBS-nanoparticles (NPs). By developing CD/DBS-NPs with different degrees of polymerization of DBS and under varied durations of ultrasonic irradiation, the correlation between particle construction and tangeretin loading ability of CD/DBS-NPs was revealed. The maximum loading amount and loading efficiency of tangeretin in tangeretin-loaded CD/DBS-NPs (TAN@CD/DBS-NPs) were 17.91 ± 0.03 μg/mg and 83.20 ± 0.15%, respectively. Furthermore, the TAN@CD/DBS-NPs displayed a homogenous distribution and satisfying stability in a series of human microenvironments, including ionic strength (physiological saline), temperature (37 °C), and acidic stomach conditions (pH 2.1). In addition, the maximum retention index of tangeretin in TAN@CD/DBS-NPs reached 99.21 ± 0.17% after a 20-day storage period, suggesting an outstanding effectiveness of CD/DBS-NPs for tangeretin preservation. The prepared nanocarriers were demonstrated to be non-toxic to cells, showing promising potential for applications in the food, pharmaceutical, and cosmetic industries.
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NEUROPHARMACOLOGY
·
2020
·
IF 4.431
Kaiyang Ma,Xiao Ding,Qiqi Song,Zhitao Han,Hang Yao,Jianhua Ding,Gang Hu
In recent years, with the discovery and research of lactate-specific receptor HCAR1(hydroxycarboxylic acid receptor 1), lactate is not only as a product of Glycolysis in astrocytes, but also as a signaling molecule which has gradually received attention. Studies have found that lactate can be used as an intercellular signaling molecule involved in synaptic plasticity , and so that peripheral administration of lactate can produce antidepressant effects. Here, we focus on HCAR1 on the most widely distributed astrocytes in the brain, found and verified that lactate could cause Arc/arg3.1 protein overexpression in astrocytes through HCAR1. However, the expression of Arc/arg3.1 does not depend on the Gi protein pathway of HCAR1, and we found that lactate enhanced the expression of Arc/arg3.1 protein through the HCAR1-β-arrestin2 pathway. In summary, lactate acts on HCAR1 of astrocytes. It enhances the expression of MAPK-dependent Arc through β-arrestin2, thereby reducing the influx of calcium ions when astrocytes are exposed to glutamate damage, achieving the role of protecting astrocytes and indirectly enhancing the absorption of glutamate by astrocytes. These results also demonstrate that HCAR1 in the brain is a potential therapeutic target in an experimental in vitro model of glutamate damage, which is strongly associated with many neurodegenerative diseases .
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Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Jiao Zou,Jin-Gang Yu
In this work, we have fabricated nafion (NF) stabilized black phosphorus nanosheets (BPNSs) and 6-O-α-maltosyl-β-cyclodextrin (G2-β-CD) composite (BPNSs-G2-β-CD) as novel electrochemical sensoring platform for chiral recognition of tryptophan (Trp) enantiomers. BPNSs-G2-β-CD composite modified glassy carbon electrode (BPNSs-G2-β-CD/GCE) was further coated with NF which served as a protective film to immobilize BPNSs-G2-β-CD on the electrode surface to achieve high stability. Under the optimum conditions, the oxidation peak current ratio of L-Trp to D-Trp (I L /I D ) and the difference between the peak potential (ΔEp = E D - E L ) were observed to be 1.49 and 20 mV at NF/BPNSs-G2-β-CD/GCE by square wave voltammetry (SWV). In addition, a linear calibration curve could be obtained for peak current versus Trp enantiomers in the concentration range 0.01–1.00 mM with detection limits of 1.07 μM and 1.71 μM for L-Trp and D-Trp (signal-to-noise ratio of 3, S/N = 3), respectively. The chiral recognition mechanism was also proposed, and the intermolecular hydrogen bonding interactions as well as the hydrophobic-cavity-triggered embedding effect dominated the effective chiral recognition. Moreover, the proposed NF/BPNSs-G2-β-CD/GCE showed excellent stability, good reproducibility and anti-interference capability. Therefore, the designed chiral sensor is expected to be practically applied for the sensitive recognition of Trp enantiomers in real samples.
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JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 4
·1105-1113
·
IF 2.479
Yuanyuan Zhong,Wenhui Li,Lidan Ran,Ran Hou,Ping Han,Shiling Lu,Qingling Wang,Wei Zhao,Yi Zhu,Juan Dong
The purpose of this study was to prepare and characterize inclusion complexes between tea polyphenol (TP) and hydroxypropyl-β-cyclodextrin (HP-β-CD), and to evaluate their antioxidant properties. Freeze-drying was used to prepare the inclusion complex of TP/HP-β-CD at different component ratios (1:0.5, 1:1, and 1:2). The supermolecular structure of the TP/HP-β-CD complex was characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). Molecular docking was used to simulate the positions and interactions of the binding sites of TP/HP-β-CD inclusion complexes and target protein receptors. In addition, the effects of TP/HP-β-CD inclusion complexes on myofibrillar protein (MP) from lamb tripe were observed under oxidative conditions. Results showed that TP was encapsulated in the cavity of HP-β-CD to form an optimal complex with 1:2 molar ratio of stoichiometry, while the FTIR, TGA, and SEM studies also support the inclusion process. Molecular modeling results were systematically analyzed to determine the stability of inclusion complexes and protein. Furthermore, the addition of an appropriate concentration (5 to 105 µmol/g) of TP/HP-β-CD inclusion complex decreased the carbonyl content, hydrophobicity, and protein aggregation of MP from lamb tripe, whereas it increased the sulfhydryl content. This improved antioxidant activity and bioavailability of the inclusion complexes will be beneficial for its potential applications in food. Practical Application Tea polyphenol was an antioxidant with potential for the field of food. In this study, the unstable properties of tea polyphenols were evaluated and were improved by inclusion of HP-β-cyclodextrin. The binding mode of the inclusion complex with protein was revealed via the molecular docking method, and the application of inclusion complex to control protein oxidation was studied. Results showed that the inclusion complex could effectively inhibit protein oxidation, which can provide a reference for the application of polyphenols in meat products and the improvement of protein properties.
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