ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Yuping Zeng,Xiangyu Gan,Zhourui Xu,Xiaoxiang Hu,Chenxuan Hu,Hanbin Ma,Hangjia Tu,Bao Chai,Chengbin Yang,Siyi Hu,Yujuan Chai
Background Sensitive and rapid antigen detection is critical for the diagnosis and treatment of infectious diseases, but conventional ELISAs including chemiluminescence-based assays are limited in sensitivity and require many operation steps. Fluorescence immunoassays are fast and convenient but often show limited sensitivity and dynamic range. Results To address the need, an aggregation-induced emission fluorgens (AIEgens) enhanced i mmunofluorescent assay with beads-based quantification on the digital microfluidic (DMF) platform was developed. Portable DMF devices and chips with small electrodes were fabricated, capable of manipulating droplets within 100 nL and boosting the reaction efficiency. AIEgen nanoparticles (NPs) with high fluorescence and photostability were synthesized to enhance the test sensitivity and detection range. The integration of AIEgen probes, transparent DMF chip design, and the large magnetic beads (10 μm) as capture agents enabled rapid and direct image-taking and signal calculation of the test result. The performance of this platform was demonstrated by point-of-care quantification of SARS-CoV-2 nucleocapsid (N) protein. Within 25 min, a limit of detection of 5.08 pg mL −1 and a limit of quantification of 8.91 pg mL −1 can be achieved using <1 μL sample. The system showed high reproducibility across the wide dynamic range (10–10 5 pg mL −1 ), with the coefficient of variance ranging from 2.6% to 9.8%. Significance This rapid, sensitive AIEgens-enhanced immunofluorescent assay on the DMF platform showed simplified reaction steps and improved performance, providing insight into the small-volume point-of-care testing of different biomarkers in research and clinical applications.
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JOURNAL OF THROMBOSIS AND HAEMOSTASIS
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2025
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IF 5
Yunchong Liu,Zhengde Zhao,Xiuyi Huang,Ying Xiao,Na Li,Wenchao Yang,Ruijia Feng,Weiqi Feng,Ting Long,Haoliang Wu,Guiyan Peng,Sifan Chen,Guangqi Chang,Kan Huang,Zilun Li
Background 2-Methoxybenzoic acid (2MOA) is a natural compound with potential salicylate-like effects; however, its impact on arterial thrombosis remains unclear. Objectives This study aimed to investigate the effects of 2MOA on thrombogenesis and its underlying mechanisms. Methods FeCl 3 -induced carotid artery injury and laser-induced cremaster artery injury thrombosis assays were used to explore the effect of 2MOA on thrombogenesis in vivo . Various ex vivo platelet function assays were conducted to evaluate the impacts of 2MOA on platelet activity. In addition, untargeted metabolomics analysis was performed to identify the alterations in intraplatelet metabolites following 2MOA treatment. Results We found that 2MOA significantly ameliorated thrombosis in a dose-dependent manner, without affecting the normal hemostasis in C57BL/6J mice. 2MOA suppressed platelet reactivity as indicated by decreased spreading, retraction, and aggregation in both mouse and human platelets. Metabolomics analysis revealed significantly alterations in purine metabolism following 2MOA treatment, which increased cyclic guanosine monophosphate production in platelets. Mechanistically, 2MOA inhibited the activity of carbonic anhydrase, leading to elevated intraplatelet cGMP level, and subsequent suppression of cytosolic phospholipase A2 phosphorylation. Conclusion Our study illustrates that 2MOA efficaciously inhibits platelet reactivity and alleviates thrombogenesis via suppressing carbonic anhydrase activity, which should be a promising reagent in the prevention and treatment of arterial thrombotic events.
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EUROPEAN POLYMER JOURNAL
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2024
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IF 5.8
Zhonglian Wu,Haidi Sun,Xu Hu,Jian Yang,Zehao Gu,Chirun Wang,Hui Chong,Jihang Dai,Hang Yao,Dong-An Wang
Osteochondral defects pose significant challenges in tissue regeneration due to the complex structure and composition of native tissue. Restoring the intricate structure and functional capacity of tissues remains a formidable task in osteochondral transplantation. In this study, a novel composite crosslinked 3D printing ink was designed to construct a biomimetic osteochondral scaffold with a sustained release bone morphogenetic protein 2 (BMP-2) hydrogel system of dopamine-modified hyaluronic acid (HAD) and BMP-2 interaction. The enzyme-linked immunosorbent assay results showed a cumulative release of BMP-2 reaching 48.3 %±6.1 % at 28 days. Theoretical calculations indicated that the binding affinity of HAD and BMP-2 was −41.8 ± 1.27 kJ/mol. These findings corroborated the outstanding sustained release efficacy of the hydrogel scaffolds due to the HAD and BMP-2 interaction. Biological function evaluations revealed that the scaffolds possessed significant osteogenesis and chondrogenesis abilities in vitro . This effect is attributed to the hydrophilic nature of the upper layer of the scaffold and the sustained release of BMP-2 from the lower layer. Ultimately, the bilayer scaffolds achieved integrated osteochondral regeneration in a rat model. These results demonstrated the potential of the 3D-printed biomimetic scaffolds with a sustained release BMP-2 hydrogel system for osteochondral repair.
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ENZYME AND MICROBIAL TECHNOLOGY
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2024
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IF 3.4
Lili Zhang,Yu Hong,Jiapeng Lu,Yi Wang,Wei Luo
Transaminases (EC 2.6.1.X, TAs) are important biocatalysts in the synthesis of chiral amines, and have significant value in the field of medicine. However, TAs suffer from low enzyme activity and poor catalytic efficiency in the synthesis of chiral amines or non-natural amino acids, which hinders their industrial applications. In this study, a novel TA derived from Paracoccus pantotrophus ( pp TA) that was investigated in our previous study was employed with a semi-rational design strategy to improve its enzyme activity to 2-ketobutyrate. By using homology modeling and molecular docking, four surrounding sites in the substrate-binding S pocket were selected as potential mutational sites. Through alanine scanning and saturation mutagenesis, the optimal mutant V153A with significantly improved enzyme activity was finally obtained, which was 578 % higher than that of the wild-type pp TA (WT). Furthermore, the mutant enzyme pp TA-V153A also exhibited slightly improved temperature and pH stability compared to WT. Subsequently, the mutant was used to convert 2-ketobutyrate for the preparation of L-2-aminobutyric acid (L-ABA). The mutant can tolerate 300 mM 2-ketobutyrate with a conversion rate of 74 %, which lays a solid foundation for the preparation of chiral amines.
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FOOD CHEMISTRY
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2024
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IF 8.5
Xueli Yang,Zihao Zong,Ben Niu,Hangjun Chen,Weijie Wu,Xiangjun Fang,Ruiling Liu,Haiyan Gao,Honglei Mu
In this study, Shiitake mushroom-derived extracellular nanovesicles (SMDENVs) were isolated from fresh Shiitake mushrooms by ultracentrifugation and sucrose gradient ultracentrifugation. The morphological characteristics of SMDENVs were investigated via Transmission Electron Microscopy and Laser Scanning Confocal Microscopy. SMDENVs were spherical, hollow, and uniform in size, with an average diameter of 177.6 ± 51.4 nm. Based on the analysis of lipidomics and proteomics, 383 lipids species and 1290 proteins were identified in SMDENVs. Compared with the conventional liposomes, SMDENVs demonstrated higher stability in different environmental conditions. Furthermore, we observed that SMDENVs were cytocompatible and inhibited the proliferation of Caco-2 cells. SMDENVs could be phagocytized by Caco-2 cells in a time-dependent manner. Further, SMDENVs also inhibited the proliferation of Caco-2 cells in a dose-dependent manner, and the half-maximal inhibitory concentration (IC 50 ) was 236.2 ± 3.2 μg/mL. Additionally, SMDENVs induced cellular apoptosis by increasing the levels of reactive oxygen species and decreasing the mitochondrial membrane potential.
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CRYSTAL GROWTH & DESIGN
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2024
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IF 3.2
Huiqin Ma,Zhuxiang Mao,Wen Liu,Yahao Zhao,Peng Liu,Difu Zhan,Qian Fu,Yizhong Huang,Changcun Han
ZnIn2S4 (ZIS) is an N-type direct bandgap semiconductor material with a visible light response, which exhibits excellent photocatalytic water-splitting performance. To enhance ZIS-based photocatalysts, heterojunction construction and cocatalyst loading methods have been explored extensively. In this research, a Co9S8/ZnIn2S4 (CS/ZIS) heterojunction was successfully synthesized via a solvothermal method, and then Ni(OH)2 nanoparticles were successfully loaded on CS/ZIS by photodeposition, yielding the Ni(OH)2/CS/ZIS photocatalyst. Our findings reveal tight coupling of ZIS nanosheets with the CS surface, establishing an effectively constructed heterojunction structure that enhances interface charge transfer and light absorption. The synergistic effect of CS and Ni(OH)2 significantly boosts the photocatalytic hydrogen production of ZIS composites. The optimum hydrogen production rate of CS/ZIS is 10.06 mmol·h–1·g–1 and that of Ni(OH)2/CS/ZIS reaches 12.92 mmol·h–1·g–1, a 11.9-fold increase over pure ZIS, with exceptional light stability. Furthermore, we discuss the photocatalytic reaction mechanism based on the experimental and characterization results. This work presents a novel approach to designing highly efficient photocatalysts using ZnIn2S4, aimed at enhancing the conversion of solar energy into chemical energy.
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CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
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2025
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IF 2
Jingyi Cao,Guangyue Wang,Yan Zhao,Guihua Zhang,Qian Wang,Hua Peng,Jie Liang,Jie Zhou,Yuning Dong,Shasha Li,Ke Jiang,Changsong Pei,Qichao Wang
Background/aims: Bladder cancer is a type of malignant tumor that disrupts normal urinary function in patients, thereby significantly impacting their quality of life. This disease also imposes a heavy economic burden on both patients and public health agencies due to high medical costs. Current common therapies, such as surgical intervention, chemical treatment, and radiotherapy, are associated with serious adverse reactions and risks of metastasis recurrence. Effective attenuation of bladder cancer proliferation and invasion remains a significant challenge. Circular RNAs have shown promise in regulating proliferation and migration of cancer cells, thus making it a potential therapeutic target for bladder cancer treatment and prognosis. This study aims to evaluate the impact of regulating circPTK2 expression on progression of bladder cancer. Methods: This research established overexpression and knock down circPTK2 models of bladder cancer cells (SW780 and UM-UC-3) primarily. Then evaluate the effect by a series of cell function test (including RT-qPCR, MTT, EdU assay, cell clone, transwell, cell cycle and cell apoptosis). Results: The findings suggest that regulated expression of circPTK2 in bladder cancer cells correlated with the abundance of mir129-5p. Meanwhile, knock down circPTK2 expression in bladder cancer cells reduced their ability to proliferate and invade; but these processes were reversed when circPTK2 expression was increased. Conclusion: In conclusion, circPTK2 may play a vital role in regulating bladder cancer progression, thereby showing potential for treatment of bladder cancer and improvement of prognosis by modulating circPTK2.
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CERAMICS INTERNATIONAL
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2025
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IF 5.6
Dongbo Zhang,Ning Wang,Xinyue Tang,Jiangrong Yan,Yongsheng Zhang
High-entropy oxides (HEOs) are promising materials for thermal barrier coatings (TBCs). In this study, a new type of TBC material, that is, (La 0.5 Gd 0.5 ) 2 (Ce 0.2 Nb 0.2 Yb 0.2 Ta 0.2 Zr 0.2 ) 2 O 7 [(LG)(CNYTZ)O] pyrochlore-structured HEO was designed and synthesized. The results showed that the structure of the (LG)(CNYTZ)O HEO synthesized at 1400 °C for 6 h was pure phase pyrochlore oxide. The microstructures of the (LG)(CNYTZ)O HEO solid specimens were studied after sintering at different temperatures (1500, 1520, 1540, 1550, 1560, 1580, and 1600 °C) for 6 h, respectively. The optimal sintering temperature was determined to be 1550 °C. The properties of the (LG)(CNYTZ)O HEO specimen sintered at 1550 °C for 6 h were tested. Its direct and indirect optical bandgap widths were determined to be 3.05 and 2.52 eV, respectively, from its UV–visible diffuse reflectance spectroscopy profile. Its infrared spectrum was recorded at 1100 °C, which confirmed its excellent high-temperature thermal radiation-shielding properties. Its thermal conductivity was determined in the temperature range of 30–1000 °C after correction for porosity, using the laser flash method, and was found to be in the range of 0.91–1.00 W m −1 K −1 . Its coefficient of thermal expansion ranged from approximately 7.47 × 10 −6 k −1 to 10.36 × 10 −6 k −1 in the temperature of 100–1000 °C. It also exhibited excellent thermophysical properties. Its hardness was in the range of 6.76–7.46 GPa, and its average hardness was measured to be 7.11 GPa using a microhardness tester, while its fracture toughness was in the range of 1.81–2.31 MPa m 1/2 , and its average fracture toughness was 2.06 MPa m 1/2 , respectively. These results indicate that the (LG)(CNYTZ)O HEO can be considered a suitable candidate material for TBCs.
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CHEMICAL PHYSICS LETTERS
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2025
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IF 3.1
Jiaying Yang,Yiman Qian,Jingqi Yan,Jiayin Li,Pengfei Liu
Advanced oxidation processes (AOPs) surpass photocatalytic methods in energy efficiency, enabling organic dye degradation under mild conditions without light activation. In this study, we crafted a highly effective Zr(WO 4 ) 2 catalyst for AOPs, rigorously characterized through SEM, TEM, TG-DTA, N 2 -sorption, XRD, and XPS analyses. The system demonstrated exceptional catalytic performance, achieving 95.62 % phenol removal (2 mg/L) within 60 min using only 0.1 g/L Zr(WO 4 ) 2 and 1 mM PMS, with singlet oxygen ( 1 O 2 ) identified as the dominant reactive species. Crucially, the synergistic redox interplay between Zr 4+ and WO 4 2− surface species emerged as the critical driver for efficient PMS activation. Beyond its catalytic prowess, the Zr(WO 4 ) 2 system exhibited remarkable reusability and versatility across multiple applications, while generating treated water with minimal toxicity, positioning it as an environmentally promising solution.
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BIOCHEMICAL PHARMACOLOGY
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2025
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IF 5.6
Shanhui Zhang,Fuhui Zhao,Changluan Pan,Yajing Wang,Zhangxiang Yang,Jing Yang,Yunniao Meng,Shaofang Lv,Jiayu Huang,Li Dong,Lei Li,Yongxi Dong
Type 2 diabetes (T2DM), which is characterized by insulin resistance and diminished sensitivity, accounts for more than 90 % of the incidence rate of diabetes. At present, the drugs used to treat T2DM in clinic are restricted due to their poor compliance or serious adverse reactions. The abnormally elevated activity of GSK-3β plays an important regulatory role in the occurrence and development of T2DM. Herein, we report the therapeutic effect of a GSK-3β covalent inhibitor N -(3-(4-benzyl-3,5-dioxo-1,2,4-thiadiazol-2-yl) phenyl) acrylamide ( GL10a ), which was discovered by our group with excellent inhibitory activity and homologous kinase selectivity. Further research had confirmed that GL10a could inhibit the activity of GSK-3β, thereby increasing the hepatic glycogen synthesis, restoring glucose homeostasis, regulating insulin signaling pathways, and improving pancreatic β-cell function with consequent increase in insulin secretion. Additionally, our findings revealed that GL10a exerted beneficial effects on gut microbiota dysbiosis in T2DM, which may contribute to its therapeutic efficacy and facilitate disease recovery. These comprehensive pharmacological properties positioned GL10a as a promising candidate, potentially offering novel therapeutic strategies and clinical options for T2DM management.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2025
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IF 6.3
Runfang Yang,Kai Zhang,Wei Yan,Chunyang Li,Yiwen Long,Maosheng Wu,Xiaolin Cheng,Hongwei Han,Xingguang Zhang,Mingxing Zhang,Guozhong Wu
Ni 3 (NO 3 ) 2 (OH) 4 positive electrode material was for the first time synthesized by gamma ray radiation under ambient conditions, and systematically investigated the synthesis mechanisms and electrochemical performance. The electrochemical performance of Ni 3 (NO 3 ) 2 (OH) 4 electrode can be regulated by controlling the absorbed dose. Ni 3 (NO 3 ) 2 (OH) 4 -200 kGy displays a remarkable electrochemical performance for supercapacitor, including a high specific capacity (1,086.7 C g –1 at 1 A g –1 ), good rate capability (50.6% at 15 A g –1 ), fast Faraday reaction kinetics, and excellent electrochemical cycle stability (90.8% retention at 5 A g –1 , after 5,000 cycles). Furthermore, the assembled Ni 3 (NO 3 ) 2 (OH) 4 -200 kGy//AC hybrid device exhibits a notable energy density of 99.8 Wh kg –1 at a power density of 849 W kg –1 (1 A g –1 ), and excellent electrochemical cycle stability, retaining ~92.5% of its initial capacitance after 5,000 cycles at 1 A g –1 . This work provides a new reaction pathway for the synthesis of inorganic materials and expands the potential application of radiation technology for electrode materials.
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DALTON TRANSACTIONS
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2025
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IF 3.3
Wei Jia,Qi He,Zhiqiang Guo,Xiang Wu
The development of electrode materials has become a top priority as the requirement of global energy. Two-dimensional Ni(OH) 2 nanomaterials hinder its application in supercapacitors due to its low conductivity and limited active sites.Herein, we synthesize Ni(OH) 2 @NiS composites by a two-step hydrothermal route. It increases the specific surface area of samples and improves the ion transport rate. The Ni(OH) 2 @NiS-2 product delivers a specific capacitance of 1778 F g -1 at 1 A g -1 and cycle stability (88% at 2 A g -1 after 10,000 times cycling). Furthermore, the asymmetric supercapacitor (ASC) achieves an energy density of 110.25 Wh kg -1 at 2700 W kg -1 . Also, the devices show stable cycling capability at extreme temperatures.
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危险品化学品经营许可证（带存储）