MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Siqi Cai,Dejun Li,Fang Luo,Zhenyu Lin,Aiwen Huang,Bin Qiu
In this work, an electrochemiluminescence (ECL) biosensor for T-2 toxin detection has been designed by combining the high specificity of a DNA hydrogel system with the convenience and sensitivity of homogeneous ECL detection. Specifically, A hydrogel was engineered through the reaction between the carboxyl group of hyaluronic acid (HA) and the amino groups of both polyethyleneimine (PEI) and DNA-modified strands, resulting in a three-dimensional network that encapsulates glucose oxidase. Glucose oxidase (GOD) is capable of catalyzing the conversion of glucose into hydrogen peroxide (H₂O₂), which is a co-reactant necessary for the chemiluminescent reagent luminol to emit light. Upon the presence of the target analyte T-2 toxin, the aptamer recognizes and binds to it, leading to the dissociation and cleavage of the hydrogel framework. This process results in the leakage of the encapsulated GOD into the supernatant. Subsequently, Adding the collected supernatant to a phosphate-buffered saline (PBS) solution containing glucose and luminol for ECL testing yields a noticeable signal change. This sensor eliminates the need for electrode modification, simplifying the operation. Within a concentration range of 10 fg/mL to 100 ng/mL, the ECL signal intensity exhibits an excellent linear relationship with the logarithm of the target concentration, with a detection limit as low as 0.32 fg/mL. The method also demonstrates excellent reproducibility and stability. The sensor demonstrated precise detection capabilities in actual milk and beer samples, a crucial aspect for research focused on specific targets.
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SENSORS
·
2025
·Vol. 25
·期 6
·1637
·
IF 3.5
Jincai Yang,Zunquan Zhao,Tianyi Ma,Jialei Bai
Point-of-care (POC) antigen detection plays a crucial role in curbing the spread of viruses. Paper-based fluorescence aptasensors are expected to offer a low-cost tool to meet the needs of decentralized POC diagnosis. Herein, we report on a fluorescent paper-based sensing system for detecting the SARS-CoV-2 spike protein. The sensing system was constructed by loading multi-layer Nb2C MXene nano-quenchers and carbon-dot-labeled aptamer (G-CDs@Apt) probes onto a mixed cellulose ester (MCE) paper substrate. On the Nb2C MXene/G-CDs@Apt sensing paper, abundant G-CDs@Apt probes were attached to the multilayer MXene nano-quenchers and kept in a fluorescence-off state, while recognition of the target detached the G-CDs@Apt probes formed the nano--quenchers, resulting in fluorescence recovery of the sensing paper. The developed paper-based sensor performed well in the one-step detection of the SARS-CoV-2 S1 protein with a detection limit of 0.067 ng/mL (0.335 pg/test). The assay exhibited good selectivity and anti-interference in the detection of the SARS-CoV-2 S1 protein in artificial saliva. Moreover, the paper-based aptasensor was successfully used to detect the SARS-CoV-2 S1 protein in actual environmental samples with recoveries of 90.87–100.55% and relative standard deviations of 1.52–3.41%. The proposed technology provides a cost-effective alternative to traditional antibody test strips for a wide range of POC diagnostic applications.
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Signal Transduction and Targeted Therapy
·
2024
·Vol. 9
·期 1
·1-13
·
IF 40.8
Yang Minghui,Li Chunhui,Ye Guoguo,Shen Chenguang,Shi Huiping,Zhong Liping,Tian Yuxin,Zhao Mengyuan,Wu Pengfei,Hussain Abid,Zhang Tian,Yang Haiyin,Yang Jun,Weng Yuhua,Liu Xinyue,Wang Zhimin,Gan Lu,Zhang Qianyu,Liu Yingxia,Yang Ge,Huang Yuanyu,Zhao Yongxiang
Emerging and recurrent infectious diseases caused by human coronaviruses (HCoVs) continue to pose a significant threat to global public health security. In light of this ongoing threat, the development of a broad-spectrum drug to combat HCoVs is an urgently priority. Herein, we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These aptamers have nanomolar affinity with the nucleocapsid protein (NP) of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and also show excellent binding efficiency to the N proteins of both SARS, MERS, HCoV-OC43 and -NL63 with affinity K D values of 1.31 to 135.36 nM. Such aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant (BA.5) with EC 50 values at 2.00 nM and 41.08 nM, respectively. The protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs (−229E and -HKU1). In conclusion, we have identified six aptamers with a high pan-coronavirus antiviral activity, which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.
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JOURNAL OF THROMBOSIS AND HAEMOSTASIS
·
2025
·
IF 5
Yunchong Liu,Zhengde Zhao,Xiuyi Huang,Ying Xiao,Na Li,Wenchao Yang,Ruijia Feng,Weiqi Feng,Ting Long,Haoliang Wu,Guiyan Peng,Sifan Chen,Guangqi Chang,Kan Huang,Zilun Li
Background 2-Methoxybenzoic acid (2MOA) is a natural compound with potential salicylate-like effects; however, its impact on arterial thrombosis remains unclear. Objectives This study aimed to investigate the effects of 2MOA on thrombogenesis and its underlying mechanisms. Methods FeCl 3 -induced carotid artery injury and laser-induced cremaster artery injury thrombosis assays were used to explore the effect of 2MOA on thrombogenesis in vivo . Various ex vivo platelet function assays were conducted to evaluate the impacts of 2MOA on platelet activity. In addition, untargeted metabolomics analysis was performed to identify the alterations in intraplatelet metabolites following 2MOA treatment. Results We found that 2MOA significantly ameliorated thrombosis in a dose-dependent manner, without affecting the normal hemostasis in C57BL/6J mice. 2MOA suppressed platelet reactivity as indicated by decreased spreading, retraction, and aggregation in both mouse and human platelets. Metabolomics analysis revealed significantly alterations in purine metabolism following 2MOA treatment, which increased cyclic guanosine monophosphate production in platelets. Mechanistically, 2MOA inhibited the activity of carbonic anhydrase, leading to elevated intraplatelet cGMP level, and subsequent suppression of cytosolic phospholipase A2 phosphorylation. Conclusion Our study illustrates that 2MOA efficaciously inhibits platelet reactivity and alleviates thrombogenesis via suppressing carbonic anhydrase activity, which should be a promising reagent in the prevention and treatment of arterial thrombotic events.
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高影响因子
Molbank
·
2024
·Vol. 2024
·期 2
·M1818
·
IF 0.6
Zhongwei Wang,Luxiao Zhou,Peng He,Yukun Qin
This study presents a novel approach in the realm of catalytic organic synthesis by integrating biomass catalytic conversion with organic synthesis techniques. UtilizingN-acetylglucosamine as the primary feedstock, the first phase of the research involves its catalytic transformation into 5-hydroxymethylfurfural (HMF). The subsequent phase employs a condensation reaction between HMF and 3,3-Dimethyl-2-butanone to synthesize a new compound, (E)-1-(5-(hydroxymethyl) furan-2-yl)-4,4-dimethylpent-1-en-3-one. This two-step process not only demonstrates the feasibility of converting biomass into valuable chemical precursors but also exemplifies the synthesis of novel compounds through green chemistry principles. The successful execution of this methodology offers fresh insights and opens new avenues for advancements in catalytic organic synthesis, emphasizing sustainability and efficiency.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Haiyan Liu,Yunfeng Bai,Jun Qin,Haiyan Wang,Yuzhen Wang,Zezhong Chen,Feng Feng
A novel sensitive fluorescence method was developed for adenosine detection by using adenosine aptamer, Exo I and a 2-aminopurine-modified DNA (2-AP probe). 2-AP probe was completely complementary to adenosine aptamer. This strategy took advantage of the high binding affinity of adenosine aptamer and the susceptibility of 2-aminopurine (2-AP) to the local base stacking environment. In the absence of the adenosine, the formation of double-stranded DNA (dsDNA) through the hybridization of the aptamer with the 2-AP probe prevented the digestion of 2-AP probe by Exo I. The fluorescence of 2-AP was significantly quenched due to the base-stacking interaction. In the presence of adenosine, the binding of adenosine with the aptamer prevented the hybridization between aptamer and 2-AP probe, leading to the digestion of 2-AP probe by Exo I and the release of 2-AP. The fluorescence was recovered. A limit of detection of 0.30 μM and a linear range from 10 μM to 600 μM ( R 2 = 0.9933) were achieved by this assay. Moreover, adenosine analogs did not produce any significant change in the fluorescence intensity response, indicating good selectivity of this sensor for adenosine detection. Finally, it was used for the diluted serum sample.
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高影响因子
IONICS
·
2024
·
IF 2.4
Yan Qiang,Zhu Wenxu,Ma Xiaoxue,Liu Na,Liu Yucheng,Xia Meichen
A series of ether-functionalized paramagnetic ionic liquids, 1-(2-methoxyethyl)-3-alkylimidazolium tetra thiocyanate cobalt [C n 2O1IM] 2 [Co(NCS) 4 ] ( n = 1, 2, 3), was synthesized and characterized. The density, surface tension, refractive index, and electrical conductivity of these ionic liquids were measured at 293.15 to 343.15 K at intervals of 5 K, and their thermal expansion coefficients α were calculated. The molecular volume was obtained by measuring the pore volume and porosity. Based on Glasser theory, the standard entropy S 0 (298 K), lattice energy U POT , surface entropy S a , and surface enthalpy, H of the ionic liquids were calculated, and the reason why the ionic liquid is in a molten state at room temperature was explained from the perspective of lattice energy. The molar surface Gibbs energy was introduced to improve the traditional Eötvös equation, which was combined with the refractive index to estimate the surface tension, obtaining a fitting index exceeding 0.99. Finally, the relationship between the electrical conductivity of the ionic liquids and temperature was investigated, and the activation energy, molar electrical conductivity, and electrical conductivity diffusion coefficient of the ionic liquids were obtained. The relationship between their properties was summarized. Compared with previously reported ionic liquids using 1-(2-methoxyethyl)-3-methylimidazolium as a cation, this type of ionic liquid has higher density and a smaller coefficient of thermal expansion.
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Materials Chemistry Frontiers
·
2025
·
IF 6.4
Xiyue Zhang,Yingjie Zhou,Minjie Hou,Xiecheng Yang,Kun Ren,Peng Dong,Jiaqian Qin,Da Zhang,Feng Liang
To meet the urgent requirement for sustainable energy storage technologies, incorporating effective waste management into the design of energy storage materials can achieve a closed-loop supply chain from waste to energy. Herein, extrinsic pseudocapacitive (PDC) cathode materials with different carbon contents were synthesized using FeSO4·7H2O waste through a simple ball milling and annealing process, which enhances sodium storage kinetics and achieves waste recycling. The results indicate that NFSO particles and graphite flakes create a disordered porous structure, which enhances the PDC effect, thus overcoming the slow migration rate of sodium-ions. Specifically, the diffusion coefficient of NFSO/C-10 increased by around an order of magnitude compared to that of NFSO, increasing from 10−12 to 10−11. The discharge capacity reached 84 mA h g−1 at 0.2C (1C = 91 mA g−1). The present work realizes the high-value reuse of waste and highlights the crucial role of the PDC effect in enhancing diffusion dynamics, which opens up a new direction for the design and synthesis of high-rate iron-based sulfate cathode materials.
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Molecular Medicine Reports
·
2024
·Vol. 29
·期 5
·1-11
·
IF 3.4
Jiayan Yang,Liangliang Dong,Yifan Wang,Lifen Gong,Hongwei Gao,Yicheng Xie
Acute lung injury (ALI) is an acute inflammatory lung disease associated with both innate and adaptive immune responses. Hexokinase 2 (HK2) is specifically highly expressed in numerous types of inflammation‑related diseases and models. In the present study in vitro and in vivo effects of targeted degradation of HK2 on ALI were explored. The degradation of HK2 by the targeting peptide TAT (transactivator of transcription protein of HIV‑1)‑ataxin 1 (ATXN1)‑chaperone‑mediated autophagy‑targeting motif (CTM) was demonstrated by ELISA and western blotting in vitro and in vivo. The inhibitory effects of TAT‑ATXN1‑CTM on lipopolysaccharide (LPS)‑induced inflammatory responses were examined using ELISAs. The therapeutic effects of TAT‑ATXN1‑CTM on LPS‑induced ALI were examined via histological examination and ELISAs in mice. 10 µM TAT‑ATXN1‑CTM administration decreased HK2 protein expression and the secretion of proinflammatory cytokines (TNF‑α and IL‑1β) without altering HK2 mRNA expression in LPS‑treated both in vitro and in vivo, while pathological lung tissue damage and the accumulation of leukocytes, neutrophils, macrophages and lymphocytes in ALI were also significantly suppressed by 10 µM TAT‑ATXN1‑CTM treatment. TAT‑ATXN1‑CTM exhibited anti‑inflammatory activity in vitro and decreased the severity of ALI in vivo. HK2 degradation may represent a novel therapeutic approach for ALI.
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xueli Yang,Zihao Zong,Ben Niu,Hangjun Chen,Weijie Wu,Xiangjun Fang,Ruiling Liu,Haiyan Gao,Honglei Mu
In this study, Shiitake mushroom-derived extracellular nanovesicles (SMDENVs) were isolated from fresh Shiitake mushrooms by ultracentrifugation and sucrose gradient ultracentrifugation. The morphological characteristics of SMDENVs were investigated via Transmission Electron Microscopy and Laser Scanning Confocal Microscopy. SMDENVs were spherical, hollow, and uniform in size, with an average diameter of 177.6 ± 51.4 nm. Based on the analysis of lipidomics and proteomics, 383 lipids species and 1290 proteins were identified in SMDENVs. Compared with the conventional liposomes, SMDENVs demonstrated higher stability in different environmental conditions. Furthermore, we observed that SMDENVs were cytocompatible and inhibited the proliferation of Caco-2 cells. SMDENVs could be phagocytized by Caco-2 cells in a time-dependent manner. Further, SMDENVs also inhibited the proliferation of Caco-2 cells in a dose-dependent manner, and the half-maximal inhibitory concentration (IC 50 ) was 236.2 ± 3.2 μg/mL. Additionally, SMDENVs induced cellular apoptosis by increasing the levels of reactive oxygen species and decreasing the mitochondrial membrane potential.
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DALTON TRANSACTIONS
·
2025
·
IF 3.3
Wei Jia,Qi He,Zhiqiang Guo,Xiang Wu
The development of electrode materials has become a top priority as the requirement of global energy. Two-dimensional Ni(OH) 2 nanomaterials hinder its application in supercapacitors due to its low conductivity and limited active sites.Herein, we synthesize Ni(OH) 2 @NiS composites by a two-step hydrothermal route. It increases the specific surface area of samples and improves the ion transport rate. The Ni(OH) 2 @NiS-2 product delivers a specific capacitance of 1778 F g -1 at 1 A g -1 and cycle stability (88% at 2 A g -1 after 10,000 times cycling). Furthermore, the asymmetric supercapacitor (ASC) achieves an energy density of 110.25 Wh kg -1 at 2700 W kg -1 . Also, the devices show stable cycling capability at extreme temperatures.
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BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Shanhui Zhang,Fuhui Zhao,Changluan Pan,Yajing Wang,Zhangxiang Yang,Jing Yang,Yunniao Meng,Shaofang Lv,Jiayu Huang,Li Dong,Lei Li,Yongxi Dong
Type 2 diabetes (T2DM), which is characterized by insulin resistance and diminished sensitivity, accounts for more than 90 % of the incidence rate of diabetes. At present, the drugs used to treat T2DM in clinic are restricted due to their poor compliance or serious adverse reactions. The abnormally elevated activity of GSK-3β plays an important regulatory role in the occurrence and development of T2DM. Herein, we report the therapeutic effect of a GSK-3β covalent inhibitor N -(3-(4-benzyl-3,5-dioxo-1,2,4-thiadiazol-2-yl) phenyl) acrylamide ( GL10a ), which was discovered by our group with excellent inhibitory activity and homologous kinase selectivity. Further research had confirmed that GL10a could inhibit the activity of GSK-3β, thereby increasing the hepatic glycogen synthesis, restoring glucose homeostasis, regulating insulin signaling pathways, and improving pancreatic β-cell function with consequent increase in insulin secretion. Additionally, our findings revealed that GL10a exerted beneficial effects on gut microbiota dysbiosis in T2DM, which may contribute to its therapeutic efficacy and facilitate disease recovery. These comprehensive pharmacological properties positioned GL10a as a promising candidate, potentially offering novel therapeutic strategies and clinical options for T2DM management.
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