Drug Design Development and Therapy
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2023
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IF 4.8
Zhen Yu,Shixin Chan,Xu Wang,Rui Sun,Ming Wang,Zhenglin Wang,Xiaomin Zuo,Jiajie Chen,Huabing Zhang,Wei Chen
Purpose To evaluate the effect of 5-fluorouracil (5-FU) combined with rutaecarpine (RUT) on the antiproliferative, anti-migratory, and apoptosis-promoting ability of colorectal cancer (CRC) cells and explore the underlying mechanism.Methods The antiproliferative effects of RUT and 5-FU on CRC cells were evaluated using MTT and colony formation assays. Anti-migration was assessed by cell scratch and transwell tests. The synergistic effect of RUT and 5-FU was assessed by isobologram and combination index analysis using CompuSyn software. The effects of RUT and 5-FU on cell apoptosis were detected by flow cytometry. Differences in protein expression levels with or without RUT and/or 5-FU treatment were assessed by Western blot. Moreover, a mouse xenograft model of CRC was established to investigate the antitumor effect of RUT and 5-FU in vivo, and Ki67 and cleaved caspase-3 expression was detected by immunofluorescence.Results In this study, we found that 5-FU combined with RUT can inhibit the proliferative, migratory, and antiapoptotic abilities of CRC cells to a significantly greater extent than either RUT or 5-FU alone both in vivo and in vitro. Western blot analysis showed that the level of signal transducer and activator of transcription 3 (STAT3) phosphorylation in CRC cells was significantly reduced after combination therapy compared with that seen with the respective monotherapies. In addition, combination therapy influenced the STAT3 signaling pathway, namely, it inhibited the expression of c-Myc, CDK4, and Bcl-2 while enhancing that of the proapoptotic protein cleaved caspase-3. Immunofluorescence staining further showed that the expression of Ki67 and cleaved caspase-3 was significantly downregulated and upregulated, respectively, in tumor tissues of mice treated with combination therapy compared with that observed with 5-FU treatment alone.Conclusion Combined therapy with 5-FU and RUT exerted a superior curative effect in CRC than treatment with either single drug alone and has potential as a novel therapeutic modality for the treatment of CRC.
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Food Bioscience
·
2023
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IF 5.2
Hongchi Zhou,Sixuan Wang,Weichao Liu,Lixuan Chang,Xuemei Zhu,Guangqing Mu,Fang Qian
This study aimed to investigate the safety and probiotic properties of Lactobacillus paraplantarum LS-5 isolated from traditional northeastern sauerkraut , and to assess the antioxidant capacity of different solvent extracts (ethanol, water, and hot water) of L. paraplantarum LS-5-inoculated sauerkraut. L. paraplantarum LS-5 was shown to have various probiotic characteristics, such as good survival ability under artificial gastrointestinal conditions, antagonistic activity of various pathogens, non-biogenic amines, antibiotic susceptibility , and non-hemolytic. The 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging activity of the L. paraplantarum LS-5-inoculated sauerkraut alcohol extract (71.13%) was significantly higher than water extract (59.59%) ( p < 0.05). The ferric reducing antioxidant power (FRAP) of sauerkraut alcohol extract (614.93 Trolox μmol L −1 ) exhibited a slightly stronger ability compared to water extract (388.53 Trolox μmol L −1 ) and the heated sauerkraut water extract (383.20 Trolox μmol L −1 ) at extract concentrations of 5.0 mg mL −1 . Its 1, 1-diphenyl1-2-picrylhydrazyl (DPPH) radical scavenging activity was also stronger. It was found by principal component analysis (PCA) and Pearson that the DPPH radical-scavenging activities of sauerkraut extracts were significantly correlated with the content of polyphenols and flavonoids ( p < 0.05). Unlike kimchi , Northeastern sauerkraut is consumed mainly by heating, so this study could contribute to understanding the antioxidant capacity of different solvent extracts of sauerkraut, and before and after heating water extracts.
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FUEL
·
2023
·
IF 7.4
Yanhong Wang,Lijiao Fan,Liqun Xiao,Lulu Wang,Dongsheng Shen,Yuyang Long
5-Hydroxymethylfurfural (HMF) has been considered a valuable biomass-derived platform chemical that can be produced by hexose dehydration in the presence of an acid. However, the yield of HMF is always reduced because of side reactions such as rehydration and condensation. In this work, the reactive adsorption process of fructose dehydration to HMF catalyzed by hydrochloric acid under microwave condition was studied. BP2000 has better adsorption capacity than activated carbon (AC) in reactive adsorption, and addition of BP2000 increases the HMF yield. The maximum theoretical yield of HMF was 65 % obtained by adding 39 g L −1 BP2000 and reacting for 4 min at 160 °C under a microwave hydrothermal condition. It was discovered that microwave hydrothermal technology (MHT) could significantly increase efficiency and reduce reaction time. Reactive adsorption could efficiently buffer HMF from side reactions when reaction conditions became severe, thereby increasing the HMF yield. Reactive adsorption provides a more sustainable method for HMF production and separation.
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Molecular Catalysis
·
2023
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IF 4.6
Xiuqin Dong,Chen Xin,Lingtao Wang,Hao Gong,Yifei Chen
Immobilization of the Rh active sites in novel porous materials has attracted attentions for solving the problems of separation and recycling of homogeneous catalysts. In this work, a comparative study of phosphine modified 1Rh/MOF-5 using three different methods was reported, namely 1Rh-P/MOF-5, 1Rh/MOF-5-P and 1Rh/MOF-5-PPh 3 . Similar with the traditional methods, PPh 3 was etched on Rh particles by impregnation in 1Rh-P/MOF-5 and encapsulated in the pores by solvothermal method in 1Rh/MOF-5-P. Moreover, we proposed a novel post-synthesis strategy for grafting phenyl phosphine on the organic linkers in 1Rh/MOF-5-PPh 3. The position of phenyl phosphine in MOFs and the effect of phosphine on the Rh active sites in hydroformylation were investigated by XRD, IR, XPS, TGA and DRIFTS. 1Rh/MOF-5-PPh 3 exhibited the best catalytic activity and stability than 1Rh-P/MOF-5 and 1Rh/MOF-5-P in the hydroformylation of 1-butene. Different P/Rh ratio in P-modified 1Rh/MOF-5 led to the spatial environment change around the Rh active sites. This study showed details in the role of phosphine ligands played in hydroformylation reactions and provided some guidance for MOFs materials in the immobilization of homogeneous catalysts.
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International Journal of Nanomedicine
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2023
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IF 8
Ning-Ke Guo,Han She,Lei Tan,Yuan-Qun Zhou,Chun-Qiong Tang,Xiao-Yong Peng,Chun-Hua Ma,Tao Li,Liang-Ming Liu
Background Intestinal barrier dysfunction is an important complication of sepsis, while the treatment is limited. Recently, parthenolide (PTL) has attracted much attention as a strategy of sepsis, but whether nano parthenolide (Nano PTL) is therapeutic in sepsis-induced intestinal barrier dysfunction is obscured.Methods In this study, cecal ligation and puncture (CLP)-induced sepsis rats and lipopolysaccharide (LPS)-stimulated intestinal epithelial cells (IECs) were used to investigate the effect of PTL on intestinal barrier dysfunction. Meanwhile, we synthesized Nano PTL and compared the protective effect of Nano PTL with ordinary PTL on intestinal barrier function in septic rats and IECs. Network pharmacology and serotonin 2A (5-HTR2A) inhibitor were used to explore the mechanism of PTL on the intestinal barrier function of sepsis.Results The encapsulation rate of Nano PTL was 95±1.5%, the drug loading rate was 11±0.5%, and the average uptake rate of intestinal epithelial cells was 94%. Ordinary PTL and Nano PTL improved the survival rate and survival time of septic rats, reduced the mean arterial pressure and the serum level of inflammatory cytokines, and protected the liver and kidney functions in vivo, and increased the value of transmembrane resistance (TEER) reduced the reactive oxygen species (ROS) and apoptosis in IECs in vitro through 5-HTR2A. Nano PTL had better effect than ordinary PTL.Conclusion Ordinary PTL and Nano PTL can protect the intestinal barrier function of septic rats by inhibiting apoptosis and ROS through up-regulating 5-HTR2A, Nano PTL is better than ordinary PTL.
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INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Miao Zuo,Qiang Niu,Yanzhong Zhu,Sijin Li,Wenlong Jia,Zheng Zhou,Xianhai Zeng,Lu Lin
This study prepared a series of biochar-based catalysts using different parts of Larix principis-rupprechtii as feedstock , to promote the 5-Hydroxymethylfurfural (HMF) synthesis from glucose and fructose. Sulfonated biochar showed high converting efficiency of fructose in both dimethyl sulfoxide (DMSO) and aqueous-natural deep eutectic solvent (A-NADES) reaction systems, with HMF yield of 96.3% prepared from the pine needle-derived acidic catalyst under mild condition. Bifunctional acidic biochar catalysts containing both Lewis acid and Brønsted acid functional groups could effectively enhance the glucose conversion with 57.2% of the HMF yield obtained in A-NADES. After the detailed characterization of the biochar catalysts, the potential mechanism of the bifunctional catalyst for the synthesis of HMF by conversion of glucose and fructose was proposed. The possible promoting mechanism of carbohydrate conversion by A-NADES through intermolecular hydrogen bonding was also explored.
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WEAR
·
2023
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IF 5
Peng Wang,Jixia Qiu,Peng Gao,Rui Dong,Yunyan Han,Mingjin Fan
The tribological and anti-corrosion performances of four as-synthesized 5-phenyltetrazole ionic liquid (PTIL) lubricant additives for water were investigated. Research results suggested that an addition 3% of PTIL lubricant additive could greatly improve the tribological properties of water. Notably, the friction coefficient was reduced by about 80% and the wear volume by about 94% for the addition of 3% of tetrabutylphosphonium 5-phenyltetrazol-2-ide (PTP 4444 ) in water. Further tested by CA, QCM, EDS , SEM and XPS showed that the exceptional tribological performances presented by PTILs were mainly due to the physical and chemical tribofilms generated during the rubbing procedure. Moreover, the synergistic effect of N-P elements also played a key role in the good tribological performances of PTP 4444 .
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DALTON TRANSACTIONS
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2023
·Vol. 52
·期 7
·1950-1961
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IF 4
Jingjing Fan,Yin Zhao,Qian Wang,Mingyu Gao,Xintao Li,Dianqing Li,Junting Feng
Aiming at the comprehensive utilization of waste carbon resources and renewable carbon resources, we put forward the photocatalytic coupling process of CO2 reduction and 5-hydroxymethylfurfural (5-HMF) oxidation mediated by the anionic compound of layered double hydroxides (LDHs). Specifically, a ZnNiFe–LDH was synthesized by co-precipitation method, during which CO2 was stored between LDH layers in the form of carbonate. Then, a certain amount of metal vacancies were introduced into LDH nanosheets by selectively etching Zn2+ ions. ICP-AES, EPR and XPS showed that the concentration of Zn vacancies gradually increased with the etching time prolonging, which thus optimized the electronic structure of LDH layers. Under the catalysis of the electron-rich metal cations and hydroxyl groups on the layers, the interlayer carbonate was in situ reduced into CO coupled accompanied with the 5-HMF oxidation to 2.5-furandiformaldehyde (DFF). Compared with the unetched ZnNiFe–LDHs, the CO and DFF yields over the LDHs etched for 3 h were increased by 2.84 and 2.82 times under UV-vis irradiation with a density of 500 mW cm−2. Finally, combined with isotope-labeled 13CO2 experiments and in situ FTIR characterization, we revealed the possible coupling mechanism and defect-induced performance enhancement mechanism.
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Phytochemistry Letters
·
2023
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IF 1.7
Lin Jia,Cui-Ping Zhang,Yi Zhu,Chun-Yan Zhao,Zeng-Hui Han,Dong-Bao Hu,Wei Shi
In an endeavor to pinpoint the structural constituents vital for the biological activity of 2,4-dihydroxy-5-methylphenyl ethanone, a series of its ether derivatives was synthesized and examined for their antifungal activities against five notable phytopathogenic fungi. The bioassay revealed that compounds 2 , 3a , 3g , 3j and 3k manifested a broad spectrum of antifungal efficacy. Notably, compound 3g was identified as especially potent, with IC 50 values ranging from 16.52 to 37.52 μ g/mL, outperforming the standard thiabendazole. The data underscore compound 3g as a prospective candidate for the development of an innovative antifungal agent. A comprehensive structure-activity relationship (SAR) study of the acetophenones was also conducted.
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International Journal of Electrochemical Science
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2023
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IF 1.5
Fang Wang,Shu Xian Zhao,Han Zeng,Wen Shan Huo
Petaloid-shapedZnO grafted with 4,5-imidazoledicarboxylic acid was proposed as a composite to entrap Hemoglobin molecules through the synergistic effect of covalent bonding and adjacent complexation between the hetero-atoms of the imidazolyl ring andtheheme sites in Hemoglobin. The morphology, structuralfeatures, and physiochemical properties ofthecomposite with Hemoglobin anchoring were investigated by photography, spectroscopy, and electrochemicalmethods, as well as the mechanism of charge shuttle of the heme protein into the composite and the dynamics of enzymaticcatalysis. Experimental results revealed that intricate interactions between components of the complex and the integrated Hemoglobin molecules alter the mechanism of charge shuttle between cofactors in the enzyme and the conductive matrix with partial heme sites in coordination with the imidazolyl ring as an intra-molecular electron relay. Such interactions arealso responsible for theprocess of substrate binding for the cofactors in the redox protein. The rapid kinetics of heterogeneous charge convection is found to be 89.0 s -1 with a unique pattern of two electrons per proton. This interactions would also lead to a lower affinity to hydrogen peroxide (893.0 μM), a high detection limit (0.9 mM), inferior sensitivity (4.5×10 -5 A•L•mol-1) and moderate kinetics of substrate attachment and transformation (1.9×10 6 and 29.8 s -1 ).
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International Journal of Electrochemical Science
·
2023
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IF 1.5
Huimin Yang,Xin Liu,Jihui Wang,Wenbin Hu
The following study is conducted to improve the corrosion resistance of magnesium alloy. First, ZnAl layered double hydroxide (ZnAl LDH) film was prepared on AZ61 magnesium alloy by using hydrothermal method. Then, as-prepared ZnAl LDH was put into 2-methylimidazole (2-mIm) solution to synthetize ZIF-8 film (ZIF-8/LDH). Finally, ZIF-8/LDH film synthesized ZIF-8-DMBIM film (ZIF-8-DMBIM/LDHs) via a shell-ligand-exchange-reaction in the methanol solution containing 5, 6-dimethylbenzimidazole (DMBIM). The surface morphology, structure, and composition of the composite film were observed and determined by scanning electronic microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and energy-dispersive X-ray spectroscopy. The wettability of composite film was measured by using a water contact angle meter, and the corrosion resistance of the composite film was estimated by the polarization curve and electrochemical impedance spectroscopy techniques. Results show that the prepared ZIF-8-DMBIM/LDH composite film has a dense hexagonal plate morphology with a two-layer structure and a thickness of 3.5 μm. Compared with ZnAl LDH and ZIF-8/LDH films, ZIF-8-DMBIM/LDH composite film has a high contact angle (115.7°), low corrosion current density, and high corrosion potential and polarization resistance due to its near-smooth surface, dense two-layer structure, and hydrophobic characters.
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EUROPEAN JOURNAL OF HISTOCHEMISTRY
·
2023
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IF 2
Zhongzhu Tang,Lei Wang,Yunwang Chen,Xiaomin Zheng,Runyu Wang,Bingxue Liu,Shiqi Zhang,Huimin Wang
Quercetin (Que) has been proven to enhance the chemosensitivity of multiple cancers, including colon cancer (CC). However, whether the combination of Que and 5-fluorouracil (5-FU) has a synergistic effect on drugresistant CC cells has not previously been reported. The effect of Que (5 and 10 μg/mL) on cell vitality and apoptosis of CC and CC drug-resistant cells was examined using a cell counting kit-8 (CCK-8) and flow cytometry. After cells were treated with 5-FU (10, 40 μg/mL), Que (10 μM, 40 μM), or 5-FU in combination with Que, cell proliferation, apoptosis, oxidative stress-related factors, reactive oxygen species (ROS), and nuclear factor erythroid 2-related factor (Nrf2)/heme oxygenase-1 (HO-1) pathway-related factors were examined by colony formation assay, flow cytometry, ELISA, ROS kit, immunofluorescence assay, and Western blot. The results showed that 5-FU reduced cell viability and induced apoptosis of CC as well as 5-FU-resistant CC cells. Que further restrained the proliferation, oxidative stress-related factors (SOD, CAT, GPx, and GR), ROS production, and induced apoptosis in CC cells and 5-FU-resistant CC cells induced by 5-FU. Moreover, the combination of Que and 5-FU attenuated the Nrf2/HO-1 pathway-related marker levels in CC cells and 5- FU-resistant CC cells. Therefore, our results suggest that Que reverses 5-FU resistance in CC cells via modulating the Nrf2/HO-1 pathway.
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