BIOMEDICAL AND ENVIRONMENTAL SCIENCES
·
2023
·
IF 3.5
Hao WU,Jiang Shan XU,Yan Hong LI,Xing Han WU,Wei HU,Meng Die LIU,Qiang SUN,Bin GUO
Objective To develop an effective treatment strategy to simultaneously avoid fatal adverse effects in the treatment of oral cancer, combination therapy has been explored because of its multiple functions. This work aims to develop a novel type of gold-nanorod-based nanomaterials decorated with tetrahedral DNA nanostructures (TDN) carrying antitumor drugs, namely, [email protected] nanocomposites. Methods In the designed structure, TDN, with a three-dimensional geometry composed of DNA strands, can provide GC base pairs for binding with the anticancer drug doxorubicin (DOX). The photothermal heating properties, biocompatibility properties, and antitumor performance of obtained [email protected] nanocomposites were investigated to assess their application potential in tumor treatment. Results Systematic studies have shown that the obtained [email protected] nanocomposites have high photothermal conversion under the illumination of an 808-nm infrared laser, leading to effective antitumor applications. In addition, the cell viability study shows that [email protected] nanocomposites have good biocompatibility. In vitro studies based on A375 cells show that the [email protected] nanocomposites can effectively eliminate cancer cells because of the combination of photothermal therapy induced by GNRS and chemotherapy induced by TDN-carrying DOX. The result shows that the obtained [email protected] nanocomposites have efficient cellular uptake and lysosome escape ability, together with their nuclear uptake behavior, which results in a significant antitumor effect. Conclusion This work has demonstrated a potential nanoplatform for anticancer applications.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2016
·Vol. 28
·期 5
·4138-4145
·
IF 1.798
Yu Mei,Meng Yanbing,Zhang Jindan,Liu Jianhua,Li Songmei
Super helical Au/TiO 2 nanocomposites were synthesized using plasmid DNA and incorporated into photoanode of dye-sensitized solar cells (DSSCs). The performance of photoanode films and DSSCs was investigated. Plasmid DNA can not only support as bio scaffold to synthesize Au/TiO 2 nanocomposites, but also act as an effective reducing agent under ultraviolet (UV) irradiation. Compared with TiO 2 -only DSSCs, the light harvesting (LH), corresponding photocurrent and power conversion efficiency (PCE) of DSSCs were enhanced by incorporating of mechanically mixed Au-TiO 2 NPs and Au/TiO 2 nanocomposites. Compared to the TiO 2 -only DSSCs samples (5.12%), the PCE of DSSCs with mechanically mixed Au-TiO 2 nanoparticles (NPs) (6.41%) increased by 25.20%, and DSSCs with Au/TiO 2 nanocomposites (8.17%) increased by 59.57%. The best PCE (8.24%) of DSSCs was obtained with the incorporation of Au/TiO 2 nanocomposites. Two reasons were presented for the best performance of DSSCs with Au/TiO 2 nanocomposites. Firstly, the localized surface plasmon resonance (LSPR) effect of Au NPs enhanced the absorption visible light. Secondly, the super helical structure of Au/TiO 2 nanocomposites provided a path for the transmission of photoelectrons.
✓ 已验证
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Lei Wang,Wenjing Zhi,Dongsheng Lian,Yu Wang,Juan Han,Yun Wang
Zeolitic imidazolate framework-8 (ZIF-8) has been successfully employed as a protective coating to encapsulate enzyme via various immobilization strategies by recent works. However, in all of the research, ZIF-8 only acts as the enzyme-immobilization matrix. In this work, by exploiting the fluorescence quenching properties of the ZIF-8 surface, we for the first time properly amalgamated the basic functionalities of ZIF-8 for horseradish peroxidise (HRP) encapsulation and fluorescence quencher by constructing HRP@ZIF-8/DNA hybrids and gave the successive detection of Hg2+ and phenol. Specifically, we introduced a biomimetic mineralization approach for facile encapsulation of HRP by ZIF-8 with high HRP-encapsulation efficiency (98.4%). Indeed, the HRP@ZIF-8 biocomposite functioned as an active biocatalyst under biologically challenging conditions, such as alkaline condition, high temperature, organic solvent, long-term storage, and continuous operation. After addition of FAM-labeled single-stranded DNA (ssDNA), the HRP@ZIF-8/DNA hybrid system could achieve rapid, specific, and sensitive detection of Hg2+ with a detection limit as low as 0.22 nM. Subsequently, benefiting from excellent catalytic activity of as-obtained HRP@ZIF-8 biocomposite, which could be successively utilized as a colorimetric sensor for sensitive measurement of phenol in micromolar level. It is believed that our strategy provides a convenient and efficient method for functionality integration of ZIF-8 without any complex modification. To the field of biosensing, our discovery broadens up the potential of bio-ZIF-8 composite for successive detection of two pollutants.
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Li Sun,Xueliang Wang,Na Zou,Hongying Li,Zhangyu Yu
The interaction of formononetin (FN) with herring sperm DNA has been studied by electrochemical methods, UV-Vis spectroscopy and fluorescence. On a poly L-cysteine film modified electrode (PLC/GCE), FN showed a couple of well-defined voltammetric peaks in 0.1 M acetate buffer of pH 5.3. As adding the herring sperm DNA into above solution, the peak current of FN decreased and no new peaks appeared. Based on the electrochemical results, the binding constant and binding ratio between FN and herring sperm DNA were determined to be 1.98×10 4 M -3 and 1:3, respectively. The UV-Vis spectroscopy indicated that the maximum absorption of FN had obviously hyperchromic effect and red shift after the interaction of DNA and FN. The interaction of DNA and FN can also make the fluorescence intensity of FN enhancement. The electrochemical and spectroscopic results indicated that the interaction of FN to DNA was an intercalative mode.
✓ 已验证
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2020
·Vol. 26
·期 50
·11604-11613
·
IF 4.857
Binggang Chen,Wenliang Wang,Xinxin Yan,Shengran Li,Sangni Jiang,Sanrong Liu,Xiaojing Ma,Xifei Yu
Graphical All-in-one : Besides the good mechanical properties of being tough and stretchable, this DNA-inspired hydrogel also exhibits both self-adhesive and strain-sensitive properties and could monitor a person's motions as good strain sensor. Hydrogels used as strain sensors often rely on splicing tapes to attach them to surfaces, which causes much inconvenience. Therefore, to develop strain sensor hydrogels that possess both good mechanical properties and self-adhesion is still a great challenge. Inspired by the multiple hydrogen bonding interactions of nucleobases in DNA, we designed and synthesized a series of hydrogels PAAm-GO-Aba/Tba/Aba+Tba comprising polyacrylamide (PAAm), graphene oxide (GO), acrylated adenine and thymine (Aba and Tba). The introduction of nucleobases helps hydrogels to adhere to various substrates through multiple hydrogen-bonding interactions. It has also been found that the adhesive strength of hydrogels with nucleobases for hogskin increased to 2.5 times that of those without nucleobases. Meanwhile, these hydrogels exhibited good dynamic mechanical and self-recovery properties. They can be directly attached to human skin as strain sensors to monitor the motions of finger, wrist, and elbow. Electrical tests indicate that they give precise real-time monitoring data and exhibit good strain sensitivity and electrical stability. This work provides a promising basis from which to explore the fabrication of tough, self-adhesive, and strain-sensitive hydrogels as strain sensors for applications in wearable devices and healthcare monitoring.
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Qian Yu,Jingshu Wang,Haibo Li,Ruiqing Li,Suyuan Zeng,Rui Li,Qingxia Yao,Hongyan Chen,Konggang Qu,Yao Zheng
In the electrocatalytic oxygen reduction and hydrogen evolution reactions (ORR/HER), metal-free carbon-based materials presented great potentials as alternatives to Pt because of their flexible adjustability of electronic and geometric properties. The pursuit of high-performance carbons demand for high-level doping, large surface area and high graphitization, but the improvement of the former two aspects would inevitably impair the third one, which seems a contradictive proposition. Herein, by a facile “mix-and-pyrolyze” method, natural DNA was first developed to synthesize N, P, S-tridoped carbons (NPSC) with porous sheet structures, high graphitization, large surface areas and abundant high-quality dopants simultaneously. Thus, the newly-prepared NPSC displays superior alkaline ORR performance comparable to Pt/C together with excellent acidic activity and better stability. Meanwhile, NPSC is one of most active acidic HER catalysts and the best alkaline HER catalyst among carbon counterparts. Density function theory computation reveled the origins of superior performance are attributed to positively charged C sites neighboring to S for ORR and P sites for HER because of DNA-derived advantaged structural features. The as-fabricated rechargeable zinc–air battery based on current tridoped carbon has closed peak power density and specific capacity but much better charge–discharge stability compared with Pt/C.
✓ 已验证
DOI
高影响因子
Science Advances
·
2021
·Vol. 7
·期 46
·
IF 14.143
Chengtao Xu,Biao Ma,Zhongli Gao,Xing Dong,Chao Zhao,Hong Liu
DNA has been considered as a compelling candidate for digital data storage due to advantages such as high coding density, long retention time, and low energy consumption. Despite many works reported, the development of a DNA-based database of full integration, high efficiency, and practical applicability is still challenging. In this work, we report the synthesis and sequencing of DNA on a single electrode with scalability for an integrated DNA-based data storage system. The synthesis of DNA is based on phosphoramidite chemistry and electrochemical deprotection. The sequencing relies on charge redistribution originated from polymerase-catalyzed primer extension, leading to a measurable current spike. By regeneration of the electrode after sequencing, repeated sequencing can be achieved to improve the accuracy. A SlipChip device is developed to simplify the liquid introduction involved in DNA synthesis and sequencing. As the proof-of-concept experiment, text information is stored in the system and then accurately retrieved.
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-11
·
IF 5.3
Liu Huamin,Li Chenghong,Wang Lina,Fang Lichao,Huang Hui,Deng Jun,Hu Yue,Li Mimi,Ran Xiaoping,Li Lulu,Zheng Junsong
A “signal-off” photoelectrochemical (PEC) sensing platform has been designed for the ultrasensitive detection of DNA methylation levels and multiple methylated sites. The platform employs tungsten trioxide and TpPa-1-COF loaded by gold nanoparticle (AuNPs@WO 3 @TpPa-1-COF) composite material as the photoactive component and p-type reduced graphene (rGO) as an efficient quencher. The PEC signal of AuNPs@WO 3 @TpPa-1-COF composite is effectively quenched in the presence of p-type rGO, because p-type rGO can compete with AuNPs@WO 3 @TpPa-1-COF to deplete light energy and electron donors. In addition, a hybrid strand reaction (HCR) amplification strategy fixes more target DNA and then combines with rGO-modified anti-5-methylcytosine antibody to facilitate ultrasensitive DNA methylation detection. Under optimal conditions, DNA methylation can be measured within a linear concentration range of 10 –14 to 10 –8 M, with an exceptionally low detection limit of 0.19 fM (S/N = 3). At the same time, the platform can conduct quantitative determination of multi-site methylation, with the linear equation △I = 44.19Log A + 61.43, and the maximum number of methylation sites is 5. The sensor demonstrates high sensitivity, excellent selectivity, and satisfactory stability. Furthermore, the proposed signal-off PEC strategy was successfully employed to detect DNA methylation in spiked human serum samples, with recoveries ranging from 93.17 to 107.28% and relative standard deviation (RSD) ranging from 1.15 to 5.49%. Graphical
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Jicong Hao, Xueyan Gong, Xueyuan Duan, Weiwei Qin, Haiying Ren, Xuping ShenTu, Zihong Yea ,Xiaoping Yu
The rapid and ultrasensitive detection of parvovirus B19 (B19V) DNA is critical for preventing severe complications in high-risk populations, such as fetal hydrops in pregnant women and aplastic crisis in immunocompromised patients. The absence of clinically approved vaccines or antivirals against B19V thus mandates the urgent development of accessible in vitro diagnostics to enable time-critical interventions and contain community transmission. Herein, we developed an electrochemical biosensor as a proof-of-concept for B19V by integrating CRISPR-Cas12a with a multivalent framework nucleic acids (FNAs), namely, 12 nm tetrahedral DNA nanostructures (TDNs). Target B19V DNA activates Cas12a to indiscriminately cleave the biotin-modified ssDNA protruded from the four vertexes of the TDNs, while the TDNs precisely orient ssDNA probes on electrodes, minimizing nonspecific adsorption. This method takes advantage of the target-specific cleavage ability of CRISPR-Cas12a (Cas12a-crRNA complex, 10-12 nm) and the unique structural and functional features of 12 nm TDNs. The comparable dimensions of the Cas12a-crRNA complex and the TDN suggest a potential synergistic effect, which contributes to the observed signal amplification and high detection sensitivity. The developed platform is user-friendly, has a low detection limit (2.19 fM), and shows high selectivity. This work establishes a foundational biosensing platform, demonstrating potential for ultrasensitive nucleic acid detection. By combining the accuracy of CRISPR-Cas12a and the benefits of FNAs, this method provides a more efficient, amplification-free, and reliable approach that holds promise for future development in point-of-care diagnostics and other applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 20
·6385
·
IF 3.5
Peng Sun,Mingbin Liu,Yongxin Zhang,Chaoran Liu,Xun Yang
Chronic hepatitis B poses a serious threat to human health and life, and early diagnosis is essential to improving patient cure rates. Hepatitis B virus (HBV) and Alpha-fetoprotein (AFP) are two key biomarkers for diagnosing chronic hepatitis B. In this study, we propose a silicon nanowire array field effect transistor (SiNW-array FET) biosensor that enables highly sensitive, real-time, and low-cost joint detection of both HBV and AFP. The SiNW-array FET is fabricated using traditional micro-nano fabrication techniques such as self-limiting oxidation and anisotropic etching, and its morphology and electrical properties were tested. The results show that the diameters of the fabricated silicon nanowires (SiNWs) are uniform and the SiNW-array FET exhibits a strong output signal and high signal-to-noise ratio. Through specific chemical modification on the surface of SiNWs, the SiNW-array FET is highly sensitive and specific to HBV-DNA fragments and AFP, with ultralow detection limits of 0.1 fM (HBV-DNA) and 0.1 fg/mL (AFP). The detection curve of the SiNW-array FET exhibits good linearity within the HBV-DNA concentration range of 0.1 fM to 100 pM and AFP concentration range of 0.1 fg/mL to 1000 pg/mL. More importantly, the device could also detect HBV-DNA successfully in serum samples, laying a solid foundation for the highly sensitive clinical detection of chronic hepatitis B.
✓ 已验证
DOI
PubMed
高影响因子
BIOMATERIALS
·
2013
·
IF 7.604
Lin Gao,Lisi Xie,Xiaojing Long,Zhiyong Wang,Cheng-Yi He,Zhi-Ying Chen,Lei Zhang,Xiang Nan,Hulong Lei,Xin Liu,Gang Liu,Jian Lu,Bensheng Qiu
Gene therapy is a very promising technology for treatment of liver diseases. Minicircle DNA (MCDNA) is a versatile gene vector , which possesses excellent features in bio-safety and duration of transgene expression. However, its application has been hampered by the lack of an efficient gene delivery system . In this study, we developed a magnetic resonance imaging (MRI) visible nanoparticle to monitor MCDNA gene delivery and explore the potential of gene therapy in vivo . The nanoparticle was constructed via self-assembly of stearic acid modified low molecular polyethyleneimine (stPEI) and superparamagnetic iron oxide (SPIO) nanocrystals . The multiple SPIO nanocrystals with a controlled clustering structure in the nanoparticles were designed to achieve high MRI sensitivity. Furthermore, the MCDNA was combined with the nanoparticles via electronic interaction. Through intrabiliary infusion, the stPEI-SPIO/MCDNA nanoparticles were efficiently delivered to liver that was visualized by MRI in vivo and confirmed by histology. Moreover, the MCDNA nanoparticles exhibited non-cytotoxicity with no obvious inflammation in the transfection sites. These results indicate that stPEI-SPIO nanoparticle is able to serve as both an efficient DNA vector delivery system and a sensitive agent for MRI visualization.
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2026
·
IF 14.1
Shuang Liang,Yiwei Tian,Feiyu Zhao,Yue Han,Kongshuo Ma,Linna Hai,Kaiqing Yun,Yueyang Zhao,Siqi Zhang,Ziyi Zhang,Yuxuan Peng,Kuan Hu,Jing Zhong,Bai Xiang,Zhaohui Wang
The cGAS-STING pathway, a critical cytosolic DNA-sensing mechanism in innate immunity, holds significant promise for cancer immunotherapy. However, conventional DNA-damaging therapies lack tumor specificity and cause damage to normal tissue. Furthermore, dendritic cells (DCs), central to the STING-mediated immune response, exhibit extrinsic immunosuppression via inhibitory receptors such as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), which impairs DNA internalization and subsequent pathway activation. Herein, we engineered a telomere stress-induced nanoreactor composed of a pH-responsive zeolitic imidazolate framework-8 encapsulating telomerase-targeted 6-thio-2’-deoxyguanosine (6-thio-dG), with TIM-3 antibodies (αTIM-3) adsorbed onto its surface. Following accumulation in the tumor, the nanoreactor degrades within the acidic tumor microenvironment, releasing 6-thio-dG to induce tumor cell-specific telomeric DNA damage. Concurrently, the αTIM-3 blocks TIM-3 receptors on DCs, thereby enhancing their internalization of the released DNA. This dual-action strategy drives robust cGAS-STING activation, enhancing type I interferon production and DCs maturation. In murine models of immunogenic and poorly immunogenic tumors, the nanoreactor significantly suppresses tumor growth and prolongs survival. By coupling tumor-intrinsic telomere stress with DC-extrinsic checkpoint inhibition, this work establishes a precision platform for cGAS-STING pathway activation, presenting a promising therapeutic strategy for telomerase-positive malignancies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）