JOURNAL OF MEDICINAL FOOD
·
2014
·Vol. 17
·期 2
·236-243
·
IF 1.699
Qisi Lin,Miao Wang,Jinghua Li,Wanping Shi,Hui Wang,Chunjie Zhao
Recineckea carnea and Tupistra chinensis collected from the Guizhou province (China) were evaluated in this study. Petroleum ether fractions from the two herbs were subjected to gas chromatography–mass spectrometry analysis; 10 species, which were fatty acids or aliphatic esters, were identified. The antimicrobial activities of a variety of extracts were evaluated against four microorganisms. The methanol extract (ME), chloroform fraction, and ethyl acetate fraction from T. chinensis exhibited antimicrobial activities comparable to standard antibiotics, whereas none of the investigated extracts from R. carnea demonstrated any antimicrobial activities. The antioxidant potential was evaluated in vitro using ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical method. The FRAP value of the ME from T. chinensis (4.19±0.088 mmol/g) was found to be significantly higher than the analogous extract from R. carnea (2.39±0.092 mmol/g); the EC50 of the ME from R. carnea (0.32±0.011 mg/mL) was found to be significantly higher than that of T. chinensis (0.30±0.015 mg/mL). Total phenolic content was estimated by the Folin–Ciocalteu's colorimetric method. A positive correlation was found between total phenolic content and antioxidant activities (FRAP value and the reciprocal of EC50). The results suggested that the phenolic compounds contributed significantly to the antioxidant capacity of R. carnea and T. chinensis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2014
·
IF 3.065
Xiangnan Li,Jiale Huang,Zidan Wang,Xinde Jiang,Wencheng Yu,Yanmei Zheng,Qingbiao Li,Ning He
The alkaline extraction and acid precipitation of longan seed phenolic compounds were investigated to develop a more practical process than currently exists to isolate these chemicals. By using an alkaline buffer as the extraction solvent, the yield of total phenols reached 46.86±0.43 mg/g after conventional solid–liquid extraction. Because of the benefit achieved from the preconcentration process, an acid precipitation efficiency of 65.29 ± 0.38% was obtained, and the separation yield of phenols reached 22.04 ± 1.95 mg/g. However, the purity of the phenolic extract was influenced by the presence in the precipitate of proteins and carbohydrates, which both moved together in all four precipitation fractions at different pH values. The FRAP assay and the assay of the radical-scavenging activity revealed the strong antioxidant activity possessed by the phenolic compounds in the precipitate extracts. This preparation process for phenolic compounds has obvious advantages, such as easy operation, safe products and low production cost, which indicated its prospective industrial potential.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Limin Xiang,Lingyun Xiao,Yihai Wang,Haifeng Li,Zebo Huang,Xiangjiu He
Moderate consumption of red wine reduces the risk of heart disease and extends lifespan, which these healthy benefits are often attributed to its high antioxidant content. The relative contributions of wine polyphenols in healthy benefits were studied in this study. Among all wine polyphenols, caffeic acid was the richest one, while gallic acid showed the highest free radical scavenging activity. There was no significant difference between the prime red wine and the red wine adding 10-fold resveratrol on neuroprotective effects on SH-SY5Y cell line. The contribution percentage of resveratrol to the antioxidant activity of red wine was less than other tested polyphenols. It suggested that resveratrol may be negligible with respect to healthy benefits of red wine.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2014
·
IF 3.096
Huiling Kong,Jixin Yang,Yifeng Zhang,Yapeng Fang,Katsuyoshi Nishinari,Glyn O. Phillips
Selenium nanoparticles (SeNPs) were prepared by using gum arabic (GA) as the stabilizer in a facile synthetic approach. The size, morphology, stability and antioxidant activity in vitro of the gum arabic-selenium nanocomposites (GA-SeNPs) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and ultraviolet–visible spectrophotometry (UV–vis). SeNPs (particle size of ∼34.9 nm) can be stabilized in gum arabic aqueous solutions for approximately 30 days. FTIR results show that SeNPs were combined to the hydroxyl groups of GA. In the present work, the alkali-hydrolyzed GA (AHGA) was also prepared and its efficiency in stabilizing SeNPs was compared with GA. It was concluded that the branched structure of GA was a significant factor for the functionality. The hydroxyl radical scavenging ability and DPPH scavenging ability of GA-SeNPs were higher than those of AHGA-SeNPs and could reach 85.3 ± 2.6%, 85.3 ± 1.9% at a concentration of 4 mg/ml, respectively.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemistry Central Journal
·
2014
·Vol. 8
·期 1
·1-7
·
IF 1.663
Luo Hui,Cai Yongqiang,Peng Zhijun,Liu Tao,Yang Shengjie
Background Hylocereus polyrhizus and Hylocereus undatus are two varieties of the commonly called pitaya fruits, and pitaya fruits have gained popularity in many countries all over the world. However, studies on chemical composition and the nutritional quality of pitaya flesh peel are limited.Results Extracts of pitaya (H. polyrhizus and H. undatus) peel were extracted by supercritical carbon dioxide extraction, and analyzed by gas chromatography–mass spectrometry analysis. Their cytotoxic and antioxidant activities were investigated. The main components of H. polyrhizus extract were β-amyrin (15.87%), α-amyrin (13.90%), octacosane (12.2%), γ-sitosterol (9.35%), octadecane (6.27%), 1-tetracosanol (5.19%), stigmast-4-en-3-one (4.65%), and campesterol (4.16%), whereas H. undatus were β-amyrin (23.39%), γ-sitosterol (19.32%), and octadecane (9.25%), heptacosane (5.52%), campesterol (5.27%), nonacosane (5.02%), and trichloroacetic acid, hexadecyl ester (5.21%). Both of the two extracts possessed good cytotoxic activities against PC3, Bcap-37, and MGC-803 cells (IC50 values ranging from 0.61 to 0.73 mg/mL), and the activities of their main components were also studied. Furthermore, these extracts also presented some radical scavenging activities, with IC50 values of 0.83 and 0.91 mg/mL, respectively.Conclusion This paper provides evidence for studying the chemical composition of supercritical carbon dioxide extracts of pitaya peel and their biological activity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2013
·
IF 3.334
Simin Feng,Zisheng Luo,Yanbing Zhang,Zhou Zhong,Baiyi Lu
Antioxidant capacities and phytochemicals such as phenolics, flavonoids, triterpenoids, phytosterols of four parts of two sugarcane cultivars have been characterised. The total triterpenoid content (TTC), total phenolic content (TPC), total flavonoid content (TFC) and total sterol content (TSC) were different among parts and cultivars of sugarcane. The node of both cultivars contained the highest TTC (2096.02 and 1779.66 mg ursolic acid/100 g DW for green-rind and red-rind sugarcane, respectively). However, the highest TPC, TFC and TSC were found in the rind. Green-rind sugarcane cultivars contained higher TSC than the red-rind sugarcane cultivars in most parts, while opposite trend were found in TPC and TFC. Stigmasterol and β-sitosterol were found as two main phytosterols. Furthermore, TPC and TFC revealed good correlations with DPPH and FRAP. As cultivars and parts could affect phytochemical content, present result may provide a theoretical basis for further exploitation of the health beneficial resources of sugarcane.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2014
·
IF 3.208
Xian Xia,Jianguo Cao,Yunxia Zheng,Quanxi Wang,Jianbo Xiao
Ferns are famous traditional medicinal plants and 300 fern species have been used as phytomedicines by Southwestern of China. However, there is little information on the phytochemicals and pharmacological characteristics of most fern species. The total flavonoid concentrations, antioxidant activity, anticancer activity, and acetylcholinesterase inhibition of extracts from 19 species of ferns were investigated. The total flavonoid concentrations ranged from 8.6 to 306.4 mg/g (w/w). The antioxidant activity including DPPH free radical scavenging, ABTS radical scavenging, and superoxide anion scavenging, reducing power and ferric reducing antioxidant potential (FRAP) of flavonoid extracts from these ferns showed a weak linear relationship with their total flavonoid concentrations. Selaginella frondosa with very low total flavonoid concentration (13.4 mg/g) showed highest inhibition against A549 cells and it is necessary to further investigate the anti-cancer compounds in S. frondosa . The extracts from Davallia cylindrica Ching and Stenoloma chusanum Ching significantly inhibited AChE activity, which illustrated that it is worthy of attention to investigate flavonoids from D. cylindrica and S. chusanum as AChE inhibitors.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Yashu Yao,Yan Xie,Chao Hong,Guowen Li,Hongyi Shen,Guang Ji
Myricetin shows low oral bioavailability (<10%) in rats due to poor aqueous solubility, though it has various pharmacological activities. Complexation with cyclodextrins (CDs) is a potent pharmaceutical method to enhance the bioavailability of poorly soluble compounds. The myricetin/HP-β-CD inclusion complex was prepared and confirmed by DSC, PXRD, and SEM. Here, the inclusion mode is described in detail with regard to structural and energetic aspects using a phase solubility diagram and 1 H NMR, NOESY, and FT-IR spectra. The water solubility and dissolution rate of myricetin were greatly enhanced by forming the myricetin/HP-β-CD inclusion complex. Consequently, the oral bioavailability of the myricetin/HP-β-CD inclusion complex in rats was effectively increased 9.4-fold over free myricetin, and its antioxidant activity was also improved. The present study provides useful information for the potential application of complexation with myricetin, a naturally occurring hydrophobic phenolic compound in herbal medicine.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Yuanqing Fu,Yan Zhang,Huiying Hu,Ying Chen,Rong Wang,Duo Li,Songbai Liu
Novel galloyl phytosterols were rationally designed by incorporation of gallic acid into phytosterols through straightforward esterification . The esterification was successfully achieved by coupling of gallic acid and phytosterols through a mild chemical Steglich esterification reaction that is more straightforward than the enzymatic method . The identity of the newly synthesized galloyl phytosterols was confirmed by NMR, HPLC–MS and IR spectroscopies . Further evaluation of the novel galloyl phytosterols with radical scavenging, ferrous ion chelating, and Rancimat methods revealed its excellent antioxidant activities that are comparable to the most potent fat-soluble antioxidants . This novel antioxidant offers an intriguing solution for naturally derived antioxidants and will have great potential application as antioxidant in food industry. The methods developed in this study will be valuable for development of other phenolic phytosterols.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2013
·Vol. 8
·期 7
·e71144
·
IF 3.73
Yihai Wang,Limin Xiang,Chunhua Wang,Chao Tang,Xiangjiu He
The antidiabetic and antioxidant activities of the ethyl acetate-soluble extract (MFE) of mulberry fruit (Morus alba L.) were investigated. In vitro , MFE showed potent α-glucosidase inhibitory activity and radical-scavenging activities against DPPH and superoxide anion radicals. In vivo , MFE could significantly decrease fasting blood glucose (FBG) and glycosylated serum protein (GSP), and increase antioxidant enzymatic activities (SOD, CAT, GSH-Px) in streptozotocin (STZ)-induced diabetic mice. Bioactivity-guided fractionation of the MFE led to the isolation of 25 phenolic compounds, and their structures were identified on the basis of MS and NMR data. All the 25 compounds were isolated from mulberry fruit for the first time. Also, the α-glucosidase inhibitory activity and antioxidant activity of the phenolics were evaluated. Potent α-glucosidase inhibitory and radical-scavenging activities of these phenolics suggested that they may be partially responsible for the antidiabetic and antioxidant activities of mulberry fruit.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2013
·Vol. 48
·期 11
·2324-2330
·
IF 1.24
Ming Cai,Xiangyang Guo,Hanhua Liang,Peilong Sun
Summary In this study, microwave-assisted extraction with ethanol (MAEE) of star anise oil from Illicium verum Hook.f . has been optimised by response surface methodology (RSM). A maximum yield of star anise oil was obtained at an optimum condition: the ratio of solvent to sample 17 mL g −1 , extraction time 16 min and microwave power 505 W. Accordingly, the highest yield of star anise oil was about 24.98%, which was much higher than that of steam distillation (SD), 7.17%. Oxygenated organic compounds in representative of trans-anethole are a major component in star anise oil, nearly 94.21% for SD and 86.66% for MAEE, identified and determined by GC-MS. The oils extracted by SD and MAEE both have strong antioxidant activities that were demonstrated by the DPPH and ABTS assays.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2013
·
IF 2.755
Wei-Xin Wang,Tian-Xiang Li,Hui Ma,Jian-Fa Zhang,Ai-Qun Jia
Ethnopharmacological relevance Smilax riparia A. DC., known as “Niu-Wei-Cai” in China, is distributed through the south and middle of China. The roots and rhizomes of Smilax riparia have been used not only as traditional Chinese medicines (TCMs) for the treatment of bronchitis , lumbago of renal asthenia , traumatic injury, asthenia edema, and cancer but also as edible wild herbs in some areas of China. Aim of the study To identify the phytochemicals in the roots and rhizomes of Smilax riparia and to investigate their antioxidant activities and cytotoxicities toward several tumor cell lines. Materials and methods Four fractions and five phenylpropanoid glycosides were obtained from roots and rhizomes of Smilax riparia under bioassay-guided screenings. The structures of five compounds were elucidated by spectroscopic methods and compared with published data. We evaluated their antioxidant activities and their cytotoxicities on five cancer cell lines: human promyelocytic leukemia (HL-60), human hepatocellular carcinoma (SMMC-7721), human lung cancer (A-549), human breast cancer (MCF-7), and human colon cancer (SW480). Results Of the five glycosides, one new compound (3, smilaside P) was isolated from an EtOAc fraction. Compound 1 was cytotoxic toward HL-60, SMMC-7721, A-549, MCF-7, and SW480 (IC 50 2.70, 3.80, 11.91, 3.79, and 3.93 μM, respectively). Moreover, compounds 1 – 3 showed moderate scavenging activities against the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC 50 339.58, 330.66, 314.49 μM, respectively). Conclusions Five phenylpropanoid glycosides were reported for the first time from this TCM. Each was studied, as observed here for the first time, in the cytotoxic experiments toward HL-60, SMMC-7721, and SW480 cell lines. Compound 1 , bearing three feruloyl groups and three acetyl groups, had the greatest cytotoxicity toward the five tumor cell lines. Compounds 1 – 3 showed moderate antioxidant activities. All results reflect that compounds 1 – 3 are cytotoxic for a wide variety of cancer cell lines of differing tissue origins and that the cytotoxicities of these compounds may be related to their antioxidant activities.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）