DRUGS IN R&D
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2024
·
IF 2.2
Jia Caiyun,Zhao Na,Song Haojing,Hu Yiting,Xu Yufang,Guo Caihui,Bai Wanjun,Dong Zhanjun
Background Ondansetron is a highly selective 5-HT3 receptor antagonist that alleviates nausea and vomiting. Bioequivalence evaluation ensures that the efficacy of generic drugs is consistent with that of the original drug. Objective The objective of this study was to evaluate the bioequivalence of ondansetron hydrochloride (HCl) tablets taken in single doses under fasting and postprandial conditions in healthy subjects. Methods In this randomized, open-label, two-cycle, crossover phase I study, liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) was used to determine the ondansetron concentration in dipotassium-ethylenediaminetetraacetate (K 2 -EDTA) plasma after the subjects received a single 8 mg of ondansetron and reference formulation. Twenty-six healthy subjects received one tablet of ondansetron under fasting conditions and 28 subjects received one under postprandial conditions. Bioequivalence was established if the 90% confidence interval (CI) was 80.00–125.00%. The pharmacokinetic parameters were calculated via WinNonLin 8.1 software and the bioequivalence data were evaluated via Phoenix WinNonlin 8.1 statistics software. Results The geometric mean ratio (GMR) of the maximum observed concentration ( C max ), the area under the plasma concentration‒time curve (AUC) from time zero to the last sampling time (AUC 0– t ), and the AUC from time zero to infinity (AUC 0–∞ ) from the test/reference formulation under fasting conditions were 90.50, 90.43, and 90.25, respectively. The 90% CIs were 83.75–97.79, 82.64–98.95, and 82.25–99.03, respectively. The GMRs of C max , AUC 0– t , and AUC 0–∞ after a high-fat meal were 96.85, 93.57, and 93.77, respectively; the 90% CIs were 88.43–106.07, 87.35–100.24, and 87.35–100.68, respectively. Conclusion The test and reference formulations of ondansetron HCl have bioequivalence for healthy adult subjects under fasting and postprandial conditions.
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高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 11
·1570-1578
·
IF 2.7
Meijuan Du,Xin Cheng,Qian Chen,Xueqin Xu
In this paper, a novel and sensitive electrochemical aptasensor for sulfadimethoxine (SDM) detection has been designed based on the triple helix structure/exonuclease I (Exo I)-assisted double signal amplification strategy. The aptamer probe (Apt) hybridizes with the signal transduction probe (STP) on the electrode to form a rigid double-stranded DNA (dsDNA) structure, so that the STP remains upright and methylene blue (MB) on the STP is far away from the electrode surface, resulting in a delicate current signal. In the presence of SDM, the SDM and Apt combine into a complex, leading to the transfer of the Apt and the exposure of the STP. Meanwhile, the added Exo I can digest the Apt to realize the cyclic amplification of SDM. After the addition of the signal probe (SP), a triple helix structure between the SP and STP is formed under acidic conditions, and MB on the STP and SP collide with the electrode surface to generate a strong electrochemical signal. The proposed aptasensor combines the features of the triple helix structure and Exo I to achieve double signal amplification for the sensitive detection of SDM with a wide linear range of 0.05–1000 ng mL−1 and a low detection limit of 0.02 ng mL−1. Furthermore, it has been successfully used to detect SDM in milk and lake water samples.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Yu Yao,Qianhong Shen,Qifeng Chen,Yanyan He,Libei Jiang,Jie Liu,Yue Li,Xudan Cao,Hui Yang
A silver phosphate (Ag3PO4)/silver (Ag)/silver halide (AgX, X = Cl, Br, or I) ternary composite photocatalysts were prepared by loading Ag nanoparticles on Ag3PO4 tetrapods followed with in situ halogenation. The interface charge transfer behavior in the Ag3PO4/Ag/AgX nanocomposite was studied, and the photocatalytic performance was also evaluated. The results show that the Ag3PO4/Ag/AgI photocatalyst exhibits higher photocatalytic activity than the other two photocatalysts due to the more matched band structure between AgI and Ag3PO4. An internal electric field (IEF) from Ag3PO4 to AgI is formed at the heterogeneous interface, so the photogenerated electrons at the conduction band of AgI can rapidly transfer to the valence band of Ag3PO4 and recombine with photogenerated holes, which is consistent with the S-scheme charge transfer mechanism. Meanwhile, the photogenerated electrons left at the conduction band of Ag3PO4 can transfer to Ag nanoparticles due to the Schottky junction and react with oxygen to produce •O2–, which is proved to be the main active species in the photocatalytic procedure. This tandem junction modulated between the S-scheme heterojunction and Schottky junction promotes the efficient separation of photogenerated carriers, thus significantly increasing the average PL lifetime of electrons by 2.95 times. Therefore, the Ag3PO4/Ag/AgI photocatalyst exhibits excellent photocatalytic activity for the degradation of methylene blue and norfloxacin. The seven consecutive cyclic tests prove the good stability of the photocatalyst, thus showing the application potential in the field of sewage treatment.
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ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY
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2024
·
IF 3
Ma Licheng,Qi Jungang,Gao Leqi,Wei Tao,Guo Yanhua
In this study, a cesium ion imprinted polymer (Cs(I)-IIP) was prepared by free radical thermal polymerization using 18-crown-6-ether (18C6) as ligand, methacrylic acid (MAA) as functional monomer, and ethylene glycol dimethacrylate (EGDMA) as crosslinking agent, which can be used for adsorption and separation of cesium ions from low-concentration solutions. The adsorption kinetic and isotherm results showed that the adsorption of Cs + fitted to the pseudo-second-order kinetic model and Langmuir model, indicating that the adsorption of Cs + on Cs(I)-IIP was the monolayer chemical adsorption. The maximum adsorption capacity was 84.21 mg·g − 1 . The selective adsorption properties are performed in Cs + , Li + , Na + , and K + multicomponent systems. The results showed that the Cs(I)-IIP has a high selectivity in the presence of coexisting Li + , Na + , and K + , and the selectivity coefficients ( K’ ) of Cs(I)-IIP for Cs + /Li + , Cs + /Na + , Cs + /K + are 2.1, 1.56, and 1.33, respectively. The high adsorption capacity and selectivity are attributed to the introduction of imprinting technology to form specific Cs + recognition adsorption sites, and the 18C6 cavity was easier to recognize Cs + in the competitive adsorption process. Finally, the Cs(I)-IIP can be regenerated and reused for 10 times with the adsorption capacity only decreased by 8.1%, indicating that the polymer has good reuse performance.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2024
·
IF 11.2
Xiaotu Yang,Zihao Yue,Ruixiang Deng,Zhengliang Zhang,Tao Zhang,Lixin Song
Stability hinders further development of all-inorganic CsPb X 3 ( X = Cl, Br, I) quantum dots (QDs) although they exhibit promising prospects in optoelectronic applications. Coating perovskite quantum dots (PQDs) with a glass network to form QD glass can significantly improve their stability. However, the dense glass network degrades their luminescent performance. In this work, the crystallization behavior of PQDs in glass and better luminescence properties are prompted by introducing titanium dioxide into borosilicate glass. The luminescence intensity of TiO 2 -doped CsPbBr 3 QD glass is increased by 1.6 times and the PLQY is increased from 49.8 % to 79 % compared to the undoped glass. Evidence proves that the improved properties are attributed to the enhanced nucleation effect of titanium dioxide during the annealing process. Benefiting from the densification of the glass network caused by titanium dioxide doping, the stability of the PQD glass is further improved. LED devices with an ultra-wide color gamut that fully covers the NTSC1953 standard and achieves 128.6 % of the NTSC1953 standard as well as 91.1 % of the Rec.2020 standard were fabricated by coupling PQD glass powder, demonstrating promising commercial applications of PQD glass in optoelectronic displays.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xueyi Cai,Ziqiang Lin,Lang Liu,Wangwang Yan,Zi Wang,Yang Zhang,Xiao Chen,Dong Liang
Momordica charantia L., commonly known as bitter gourd, is a popular vegetable and medical herb in many countries. The most characteristic phytochemical profile of bitter gourd is its high content of cucurbitane-type triterpenoids, which are responsible for its bitter taste and therapeutic potential. More than 260 cucurbitane-type triterpenoids, including at least 11 5,19-hemiacetals, 40 5,19-methyl acetals, 46 methyl ethers, 6 5,19-ethyl acetals, and 7 ethyl ethers, have been identified from bitter gourd. In this work, by studying the chemical transformation of momordicine I, one of the most abundant cucurbitane-type triterpenoids in bitter gourd, we showed that under acidic conditions, 5,19-hemiacetals of cucurbitane-type triterpenoids could be formed from the intramolecular nucleophilic addition between the C-19 aldehyde and the C-7 hydroxyl group; furthermore, 5,19-acetals and methyl ethers could be generated if methanol was available. Using a UPLC-MS/MS quantification method, we showed that these momordicine I derivatives could not be detected from bitter gourd fruits when extracted with a nonmethanolic solution but could be detected when extracted with methanol after thermal treatment. Moreover, these momordicine I derivatives were detected in two of eight brands of bitter gourd dietary supplements. This might be concerning because these derivatives were not the natural components of bitter gourd, and their safety and efficacy profiles were unknown. Besides, the transformation reactions reported herein should apply widely to other cucurbitane-type triterpenoids as well. Therefore, we believe all 40 5,19-acetals, 46 methyl ethers, 6 5,19-ethyl acetals, and 7 ethyl ethers were the extraction and isolation artifacts and should not be regarded as the natural components of bitter gourd. This work will help to clarify the current misunderstanding of the real cucurbitane-type triterpenoid profile of bitter gourd.
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REACTIVE & FUNCTIONAL POLYMERS
·
2025
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IF 5.1
Lin Yang,Xueling Liu,Bingyue Song,Sixian Yang,Xiaojie Zhang,Chong Zhao,Yixiao Wu,Lei Zhang,Yanjun Huang
Effective and selective recovery of precious metal silver (Ag(I)) from wastewater can promote resource recycling and mitigate environmental pollution. In this study, dimercaptosuccinic acid (DMSA) functionalized polyhedral oligomeric silsesquioxane (POSS)-based hybrid polymer (DPHP) was synthesized and employed for the adsorption of Ag(I) from aqueous solution. The obtained adsorbent was characterized using SEM-EDS, XPS, solid-state 13 C NMR, and 29 Si NMR spectra. Batch adsorption experiments were conducted to evaluate the adsorption performance of DPHP for Ag(I). The results revealed that the maximum adsorption capacity of DPHP for Ag(I) was 494.65 mg g −1 at pH 4, and the adsorption process could be well described by pseudo-second-order and Sips models. Thermodynamic studies implied the adsorption of Ag(I) was an endothermic and spontaneous adsorption process in nature. Adsorption and desorption experiments demonstrated that the regeneration efficiency of DPHP remained above 83 % after five cycles and the preconcentration factor of DPHP was determined to be 125. Furthermore, DPHP exhibited selectivity to Ag(I) in solutions containing a variety of competitive metal ions and simulated industrial wastewater. Based on density functional theory (DFT) calculations, as well as XPS, FTIR, and Raman analyses, the interaction between Ag(I) and DPHP involved electrostatic attraction, ion exchange, and coordination, with the coordination mainly between thioether and carboxy groups and Ag(I) ions. Overall, DPHP is a promising adsorbent for the recovery of Ag(I) from aqueous solutions.
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ACS Applied Nano Materials
·
2025
·
IF 5.5
Xinran Li,Yuwei Zhong,Habib Ullah,Jian Luo,Lee Li,Ghafar Ali,Alaa G. M. Osman,Xiujian Zhao,Yi Xie
The intrinsic structural lability and limited processability of inorganic halide perovskites heavily hamper their commercial applications. Herein, we develop a facile and scalable room-temperature (RT) water-based procedure for the synthesis of Na–Cu-codoped CsPbIxBr3–x-embedded Pb(OH)I (denoted as the Na–Cu–CsPbIxBr3–x@Pb(OH)I) composite to overcome the above-mentioned bottlenecks. Compared to Na doping, the codoping with Na and Cu leads to a slight shift of the photoluminescence (PL) peak position from ∼612 to 622 nm, increases the PL intensity by 32.5 times, and improves the PL quantum yield from 1% to 5%. Notably, the optimized Na–Cu–CsPbIxBr3–x@Pb(OH)I composite displays outstanding stability under various harsh environments such as soaking in RT water or hot water (up to 90 °C) and annealing at high temperature, which indicates that Pb(OH)I can act as an effective water-blocking and protective encapsulation layer. It is more interesting that the photoluminescence (PL) intensity of Na–Cu–CsPbIxBr3–x@Pb(OH)I can be significantly increased to 2.17 and 1.29 times after soaking the sample in RT water for 35 and 105 days, respectively, and 1.25 times even after immersing in hot water (90 °C) for 6 h, indicating an improved optical property. Meanwhile, the crystal structure and emission wavelength are maintained. The insight of this work is quite beneficial for designing highly stable inorganic perovskite-based nanomaterials for realization in light-emitting devices and other optoelectronic devices.
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Journal of Translational Medicine
·
2025
·Vol. 23
·期 1
·1-19
·
IF 7.5
Xing Chunyang,Hu Wei,Zhao Liyuan
Background Osteosarcoma (OS) is one of the most common malignancies arising in bone. Hypoxia and immune regulation are pivotal in tumor biology. However, their combined effects and mechanisms in OS remain understudied. This study aimed to explore the role and mechanism of hypoxic-induced M2 macrophages in promoting the progression of OS.Methods Differentially expressed proteins in hypoxic macrophage supernatants were detected by antibody array. Cell functional experiments, siRNA-mediated gene silencing, and overexpression transfection were used to study osteopontin (OPN) and its supernatant effect. Bioinformatics analysis was applied to investigate downstream targets and pathways, and a xenograft model was established to assess in vivo effects.Results Our data revealed that hypoxic M2 macrophage supernatant enhanced OS malignancy and epithelial-mesenchymal transition, activating cancer pathways. Hypoxia upregulated OPN in M2 macrophages, and OPN inhibition reduced its tumor-promoting effect. Early growth response 3 (EGR3) was differentially expressed in OS cells treated with the supernatant, and its overexpression inhibited OS cell migration, reversing tumor promotion. Interferon-stimulated gene 15 (ISG15), a key differentially expressed gene related to OPN and EGR3 overexpression, inhibited OS cell proliferation and migration. Additionally, OPN increased retinoic acid-inducible gene I (RIG-I) expression and enhanced signal transducer and activator of transcription 3, nuclear factor kappa B, and extracellular signal-regulated kinase signaling, while EGR3 and ISG15 overexpression inhibited these effects. Silencing ISG15 restored pathway activation and reversed the inhibitory effect of EGR3 on OS cell migration. Dual-Luciferase reporter gene assay confirmed that EGR3 activates ISG15 transcription. OPN treatment upregulated DNA (cytosine-5)-methyltransferase 1 (DNMT1) expression, and ChIP assays demonstrated that EGR3 overexpression enhanced DNMT1 binding to the EGR3 promoter. These findings suggest that OPN promotes OS malignancy by downregulating EGR3 and ISG15, and by enhancing RIG-I expression, as validated in a xenograft model of OS.Conclusion Our findings demonstrate that hypoxic-induced M2 macrophages promote OS progression through OPN-dependent mechanisms, including inhibition of EGR3 and ISG15 expression and upregulation of RIG-I.Graphical abstract
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Environmental Chemistry and Ecotoxicology
·
2025
·
IF 8.2
Gao Feiyue,Zhou Chuncai,Li Chen,Xu Shihai,Li Zhiguo,Li Xin,Liu Guijian
The novel ultra-micro porous carbon sheet (PCs) with exceptionally high surface area (3034 m 2 /g) were synthesized through green potassium citrate (K 3 C 6 H 5 O 7 ) activation for efficient thallium(I) [Tl(I)] removal from wastewater. The static adsorption results revealed that PCs achieved exceptional removal efficiencies above 95 % across pH 6–12, maintained high selectivity against competing ions (K + , Na + , Cl − , F − ) and organic interferents, and retained above 80 % capacity after five regeneration cycles. Adsorption followed Pseudo-second order kinetics and Freundlich isotherm models, indicating multilayer adsorption on heterogeneous surfaces, with thermodynamics confirming spontaneous exothermic processes. The density functional theory (DFT) calculation results demonstrated that lone pair electrons involved in complexation and π-electrons participating in coordination were supplied by oxygen-containing functional groups and aromatic rings, respectively. The sorption mechanisms included surface complexation, ion exchange, electrostatic attraction, and cation-π interaction. The column sorption results demonstrated the influent flow rate, bed height, and initial Tl(I) concentration-dependent removal performance. The Tl(I)-containing wastewater at ppm level could meet the discharge standard by optimizing influent flow rate and bed height in column adsorption. The dynamic sorption process was described properly by Thomas and Yoon-Nelson models. This study provides a systematic theoretical foundation for designing porous carbons in Tl-contaminated water remediation.
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ACS Nano
·
2025
·
IF 16
Hui Wang,Weitao Yang,Yan Xu,Weiwei Zeng,Dinghua Liu,Tianming Cui,Yanjing Yun,Chang Liu,Zhuoyao Wu,Bingbo Zhang
Monovalent copper (Cu(I)) drives cuproptosis and catalytic therapy, yet its rapid oxidation reduces the activity. We developed bioinspired Cu(I)-bovine serum albumin single-atom nanozymes (Cu(I)-BSA SAzymes) via mild biomineralization, achieving stability (>90 days) and dual enzyme-like activities (peroxidase (POD)/catalase (CAT)) to convert tumor H2O2 into cytotoxic reactive oxygen species (ROS) while alleviating hypoxia. These SAzymes deplete glutathione to amplify oxidative stress and release Cu(I), which aggregates dihydrolipoamide S-acetyltransferase (DLAT) to induce cuproptosis─distinct from apoptosis/ferroptosis. Mechanistically, transcriptomics analyses suggest that Cu(I)-BSA SAzymes exerted their effects by regulating the oxidative stress and energy metabolism in 4T1 cells. The tumor microenvironment further mediates SAzymes aggregation and in situ transformation into paramagnetic Cu(II), enabling self-amplified T1-weighted MRI contrast for real-time activation monitoring. In breast (4T1) and colon (CT26) tumors, Cu(I)-BSA SAzymes suppress growth by 68.8% and 80.3% ([Cu] = 4 mg kg–1). This study reports a stable Cu(I) platform for catalytic-plus-cuproptosis theranostics.
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Comparative Immunology Reports
·
2025
·
IF 0
Dandan Feng,Zhu Zhu,Shangpeng Wang,Yangcan Gao,Yingwen Li,Yuying Sun,Jiquan Zhang
Antimicrobial peptides (AMPs) are crucial immune effectors in the defense against pathogens. Crustins, small molecule antimicrobial peptides found in crustaceans, exhibit antimicrobial and antiviral properties, microbial binding ability, biofilm growth inhibition, and protease inhibition. In this study, we identify and characterize a crustin (named NdCrus1) from Neocaridina denticulata sinensis . The NdCrus1 was expressed in all tested tissues, with the highest expression in the stomach. NdCrus1 shares a high identity with type I crustins and contains a whey acidic protein (WAP) domain, featuring eight cysteine residues that form the conserved ‘‘four-disulfide core’’ structure. Recombinant NdCrus1 (rNdCrus1) did not significantly inhibit Bacillus subtilis and Vibrio parahaemolyticus but possessed an inhibitory effect on proteases under a wide range of temperature conditions. In addition, in vitro binding assays and molecular docking results indicated that NdCrus1 had a strong binding capacity with chitin. These results provide new insights into crustins in the life activities of N. denticulata sinensis and provide references for studying antibacterial activity characteristics and mechanisms in other crustaceans.
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