INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 21
·12788
·
IF 6.208
Chichang Chen,Ye Wang,Li Xu,Siwen Wang,Xiangning Chen,Yuanyue Shen
The surface of fresh-cut carrots is apt to white blush, however the physiological and molecular mechanism for this process is not yet fully understood. In this study, exogenous abscisic acid (ABA) and ethylene separately promoted and inhibited the white-blush formation after three days after treatment, respectively. Metabolome analysis found that white-blush components mainly consist of p-hydroxyphenyl lignin and guaiacyl lignin. Transcriptome analysis found an increase in the whiteness values was consistent with the higher expression of genes encoding O-methyltransferase, trans-anol O-methyltransferase, bergaptol O-methyltransferase, caffeic acid 3-O-methyltransferase, phenylalanine ammonia-lyase, and ferulate-5-hydroxylase, together with the lower expression of genes encoding cinnamic acid 4-hydroxylase caffeoyl-CoA O-methyltransferase and 5-O-(4-coumaroyl)-D-quinate 3′-monooxygenase. In conclusion, ABA plays an important role in lignin biosynthesis essential to the formation of white blush in fresh-cut carrots. This is the first report that uncovers the physiological and molecular causes of white blush in fresh-cut carrots, providing a basis for white-blush control in fresh-cut carrots.Keywords:white blush;carotenoids;lignin;molecular mechanisms
研究领域:
植物生理学
·
分子生物学
·
食品科学
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DOI
PubMed
高影响因子
NMR IN BIOMEDICINE
·
2026
·Vol. 39
·期 4
·e70249
·
IF 2.8
Xiaomin Duan,Longyang Xiao,Haichen Zhao,Yuanxiang Gao,Lili Wang,Haiyang Yu,Xiaoyuan Zhou,Xiaojuan Shi,Zhiming Li
This study aimed to validate the feasibility of hypoxia-targeted magnetic resonance imaging (MRI) molecular probe (CAIX-Gd) for evaluating hypoxia status in rats with hepatic fibrosis. A macrocyclic ligand compound (Compound 3) was synthesized using the carbonic anhydrase IX (CAIX) inhibitor acetazolamide as the targeting moiety, and its structure was confirmed by 1 H-NMR spectroscopy. CAIX-Gd was then prepared by chelating gadolinium, with free gadolinium content quantified via the xylenol orange assay. In biocompatibility tests, CAIX-Gd administration showed no significant histopathological changes compared with saline controls. For hypoxia detection, rats were divided into three groups: (A) hepatic fibrosis (HF) with CAIX-Gd, (B) HF with DTPA-Gd, and (C) normal controls (NC) with CAIX-Gd. The difference in T1 relaxation time (∆T1) pre- and post-contrast was measured in regions of interest (ROI). HF rats showed increased collagen deposition (Masson staining), α-SMA activation, and elevated hypoxia markers (PIMO/HIF-1α) compared to controls (all p < 0.05). In group C, mild ∆T1 shortening was seen at 10 min post-injection. While ∆T1 continuously decreased in group B, the shortening was significantly greater in group A ( p < 0.05), peaking at 10 min. In conclusion, the CAIX-Gd probe was successfully synthesized and demonstrated potential for the non-invasive MRI detection of hypoxia in hepatic fibrosis.
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Wenxing Xin,Qian Wang,Dong Yang,Ao Yan,Yiwei Yu,Enfeng Zhang,Shaojie Qin,Xiping Zhang,Ziyi Zhu,Yiyong Zhang,Xue Li
Lithium metal batteries suffer from unstable interphases due to uncontrolled dendrite growth and continuous electrolyte depletion. Research indicates that constructing a solid electrolyte interphase (Hereinafter referred to as SEI) film enriched with lithium fluoride (LiF) can effectively suppress lithium dendrite growth and reduce the occurrence of side reactions. However, existing research has primarily focused on the formation mechanism of LiF and its concentration within the SEI, while neglecting a critical parameter in the film formation kinetics—the LiF formation rate. This limitation has resulted in a lack of effective strategies for rapidly forming high-performance SEIs rich in LiF. This work introduces a cobalt-based organometallic salt additive, wherein the exchange reaction between cobalt and lithium ions in situ generates a catalytic metallic cobalt layer. Through active sites on the cobalt layer surface, TFSI − anions are strongly adsorbed and catalyzed to decompose at the interphase. This layer synergistically optimizes the electrolyte to promote LiF formation. This strategy effectively accelerates TFSI − decomposition, hastens Li + desolvation, reduces nucleation overpotential, and guides uniform lithium deposition/stripping, thereby significantly suppressing dendrite propagation. Consequently, the Li||Cu half-cell achieves a Coulombic efficiency of up to 99.77% under the Aurbach test; while the Li||LFP full cell retained 72.37% capacity after 400 cycles under stringent conditions (N/ P = 1.1, 1 C). This site-specific catalytic strategy provides an effective pathway for constructing stable inorganic-rich SEI layers, paving a promising road toward developing safe and durable lithium metal batteries.
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2026
·Vol. 61
·期 8
·5353-5368
·
IF 3.9
Wang Rui,Pang Guoli,Zhu Yuping,Liu Yiming,Zhang Qiang,Ji Yongfeng,Dong Lili,Lei Tingzhou,Ren Suxia
Ideal double-layer capacitor electrodes require both high specific surface area and a porous structure efficiently wetted by electrolyte ions. Therefore, precisely controlling the pore structure of porous carbon materials to synergistically enhance both specific surface area and ion transport efficiency has become a key research challenge. This study employs lignin as a renewable carbon source, utilizing a templating approach to controllably synthesize hierarchical porous carbon materials. It systematically investigates the effects of single MgO hard templates, MgO-P123 dual templates, and single P123 soft templates on material structure and electrochemical performance. The MgO templating agent primarily contributes macropores/mesopores, enhancing ion transport and structural stability; the P123 templating agent mainly contributes mesopores, providing an efficient transport network and high specific surface area; the MgO and P123 dual-templating agents synergistically construct a multi-level pore structure comprising micropores, mesopores, and macropores. The ELCP-0.25 porous carbon prepared using P123 exhibits a high specific surface area and hierarchical porous structure, demonstrating outstanding performance in electrochemical energy storage applications. Within a three-electrode test system, this material displays exceptional electrochemical properties in KOH electrolyte, achieving a specific capacitance of 430 F/g at a current density of 1 A/g. Based on ELCP-0.25, symmetrical supercapacitors assembled with KOH and Et 4 NBF 4 /PC as electrolytes exhibit high specific capacitance and excellent cycle stability of 343 F/g and 161.84 F/g, respectively, with capacitance retention rates of up to 99.33% and 76.64% after 10,000 charge–discharge cycles. This work demonstrates the advantages of the template method in constructing hierarchical porous carbon materials, providing an effective technical approach for converting biomass waste into electrode materials for energy storage devices.
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Ge Yan,Yuting Wang,Ji Ni,Ying Jiang,Jinping Liang
Value-added utilization of biowastes has been a research hot spot in waste-to-resource field. However, the roles of the self-confined trace elements in biowaste-derived carbon have been frequently neglected. Herein, using mango seeds-derived carbon (MSC) as a proof of concept, the pivotal roles of self-confined trace elements in peroxymonosulfate (PMS) activation toward Rhodamine B (RhB) removal were rectified. The selected mesoporous MSC 1000 (carbonization at 1000 °C), with more than 10 trace elements (Ca, O, K, Mg, Si, Fe, Cu, etc.), had an ultrahigh BET specific surface area of 1903 m 2 /g. It presented outstanding adsorption capacity, which, in combination with remarkable activity for PMS activation, facilitated total RhB removal within 60 min. Quenching experiments, EPR tests and chemical probes collectively revealed that high-valent metal-oxo species, e.g., Fe(IV) O, generated from self-confined trace metal/metal oxides, dominated RhB mineralization. Furthermore, the secondary 1 O 2 was also produced at pyridinic N and graphite N active sites. In addition, MSC 1000 had prominent adaptability to complicated water matrices. Surprisingly, regeneration by recalcination can improve catalyst’s performances. This study help recognize the critical roles of trace components in carbonaceous catalysts in Fenton-like oxidation toward wastewater decontamination.
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DOI
高影响因子
Frontiers in Plant Science
·
2026
·
IF 4.8
Zhiheng Feng,Peijun Li,Guang Yang,Yining Wang,Mengqi Li,Jiangting Wu,Nan Chao,Li Liu
Proanthocyanidins (PAs), polymers of flavan-3-ols, are crucial for the sensory quality and stress defense of mulberry (Morus spp.). The biosynthesis of their monomers, catechin and epicatechin, are catalyzed by leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), respectively, representing key rate-limiting steps that determine PA composition and abundance. In this study, systematic functional analyses including phylogenetic analysis, quantified spatio-temporal expression profiles during fruit development (S1-S4 stages), in vitro enzymatic assay, knock-down using Virus-Induced Gene Silencing (VIGS) in mulberry leaves and heterologous overexpression in Arabidopsis thaliana were conducted to reveal their roles in proanthocyanidin biosynthesis in mulberry. Results showed that MaLAR (969 bp) and MaANR (1014 bp) were successfully cloned and phylogenetically conserved. Spatio-temporal expression analysis revealed distinct patterns: MaLAR expression continuously increased, reaching highest expression level at the fully ripe stage (S4), whereas MaANR showed highest expression level at the color-turning stage (S2). In vitro enzymatic assays confirmed that MaLAR catalyzed the formation of catechin from leucoanthocyanidin, and MaANR catalyzed the formation of epicatechin from anthocyanidin. VIGS-mediated silencing of either gene in mulberry leaves led to significant reduction in total PA content. Conversely, heterologous overexpression of MaLAR or MaANR in Arabidopsis resulted in significant increase of PA in the seed coat. Our findings confirm that MaLAR and MaANR are conserved, key positive regulators of PA biosynthesis in mulberry. The differential expression patterns during fruit ripening suggest they play distinct, temporally regulated roles in determining the final PA composition and content. These results provide an important theoretical basis and represent important targets for the metabolic engineering and molecular breeding of mulberry for improved fruit quality.
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产品关联
DOI
PubMed
高影响因子
GENE
·
2015
·
IF 2.138
Ping Chen,Jiying Wang,Haiyin Li,Yan Li,Peng Chen,Tian Li,Xi Chen,Junjie Xiao,Liang Zhang
In insects, pigment patterns are formed by melanin, ommochromes, and pteridines. Here, the effects of pteridine synthesis on melanin formation were studied using 4th instar larvae of a wild-type silkworm strain, dazao ( Bombyx mori ), with normal color and markings. Results from injected larvae and in vitro integument culture indicated that decreased activity of guanosine triphosphate cyclohydrolase I (GTP-CH I, a rate-limiting enzyme for pteridine synthesis), lowers BH4 (6R- l -erythro-5,6,7,8-tetrahydrobiopterin, a production correlated with GTP-CH I activity) levels and eliminates markings and coloration. The conversion of phenylalanine and tyrosine to melanin was prevented when GTP-CH I was inhibited. When BH4 was added, phenylalanine was converted to tyrosine , and the tyrosine concentration increased. Tyrosine was then converted to melanin to create normal markings and coloration. Decreasing GTP-CH I activity did not affect L-DOPA (3,4- l -dihydroxyphenylalanine). GTP-CH I affected melanin synthesis by generating the BH4 used in two key reaction steps: (1) conversion of phenylalanine to tyrosine by PAH (phenylalanine hydroxylase) and (2) conversion of tyrosine to L-DOPA by TH (tyrosine hydroxylase). Expression profiles of BmGTPCH Ia , BmGTPCH Ib , BmTH , and BmPAH in the integument were consistent with the current findings.
研究领域:
昆虫学
·
生物化学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 10
·期 4
·598-606
·
IF 6.331
Shuai Wu,Xiaoyang Cheng,Xiaomu Xu,Jiacheng Wu,Zhiqiang Huang,Zhenzhao Guo,Ping He,Changren Zhou,Hong Li
In this work, the role of chitosan (CS) in improving the properties of bioactive glass (BG) paste for wound healing was studied. Based on in vitro evaluation, it was found that the addition of CS neutralizes the pH value from 11.0 to 7.5, which did not lead to decreasing the bioactivity of BG paste in vitro. The rheological properties showed that the composite paste had higher bio-adhesion and better affinity with the skin surface than either CS or the BG paste. The antibacterial property evaluation showed that the composite paste had stronger antibacterial activity than either CS or BG paste and promoted the proliferation of HUVECs (human umbilical vein endothelial cells) and HaCat (human immortalized keratinocyte cells). Comparatively, the effect of promoting the proliferation of HUVECs is more significant than that of HaCat. The burn-wound model of rat was developed for evaluating in vivo activity, and the addition of CS effectively promoted wound healing without obvious inflammation according to the IL-1β and IL-6 staining. This novel paste is expected to provide a promising alternative for wound healing.
研究领域:
材料科学
·
生物学
·
医学
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DOI
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2020
·Vol. 192
·期 6
·1-11
·
IF 1.903
Zagui Guilherme Sgobbi,de Andrade Leonardo Neves,Moreira Natália Columbaro,Silva Thaís Vilela,Machado Gabriel Pinheiro,da Costa Darini Ana Lúcia,Segura-Muñoz Susana Inés
Multidrug resistance mediated by β-lactamase in Gram-negative bacilli is a serious public health problem. Sewers are considered reservoirs of multiresistant bacteria due to presence of antibiotics that select them and favor their dissemination. The present study evaluated the antibiotic resistance profile and β-lactamases production in Gram-negative bacilli isolates from hospital sewage and urban wastewater treatment plants (UWWTP) in Brazil. Bacteria were isolated and identified with biochemical tests. Antibiotic susceptibility testing was performed by the disk-diffusion method and detection of extended-spectrum β-lactamase and carbapenemases by enzymatic inhibitor and conventional PCR. Differences in resistance to amoxicillin clavulanic, aztreonam, cefepime, and cefotaxime were observed in hospital sewage compared with urban sewage ( p < 0.05). The multidrug-resistant phenotype was observed in 33.3% of hospital sewage isolates ( p = 0.0025). β-lactamases genes were found in 35.6% of isolates, with the most frequent being bla KPC and bla TEM (17.8%), and bla SHV and bla CTX-M (13.3% and 8.9%, respectively). The data obtained are relevant, since the bacteria detected are on the priority pathogens list from the World Health Organization and hospital sewage could be released untreated into the municipal collection system, which may favor the spread of resistance. Changes in hospital sewage discharge practices, as well as additional technologies regarding effluent disinfection in the UWWTP, can prevent the spread of these bacteria into the environment and negative impact on water resources.
研究领域:
微生物学
·
公共卫生
·
环境科学
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DOI
高影响因子
Systems
·
2022
·Vol. 10
·期 1
·16
·
IF 2.895
Chunmei Zhang,Ziwen Feng,Qilong Ren,Wei-Lng Hsu
Ecological land has two definitions: the broad category and the narrow category. This paper studied the narrow category, which refers to the land whose main purpose is to provide ecological services, such as environmental regulation and biological conservation. Ecological land areas are likely to decrease with the development of urbanisation all over the world, which poses potential risks to the environment, society, and public health, and low utilisation efficiency of ecological land will exacerbate this risk. Based on the actual input–output situation of ecological land utilisation, we constructed an index system in the Yangtze Delta to measure the ecological land utilisation efficiency with a Slack-Based Model (SBM) in the data envelopment analysis (DEA). The results revealed that the comprehensive efficiency exhibited an increasing trend from 2012 to 2020, and more high-efficiency cities appeared in the areas closer to Shanghai. Both the technical efficiency and scale efficiency increased over the research period. The technical efficiencies in most cities were efficient and equal to the scale efficiencies. Among the remaining cities, the technical efficiencies and scale efficiencies were inefficient and not synchronous. In addition, we found that the that poor efficiencies of some cities can mainly be attributed to low-technology, an unsuited-scale, or a combination of these. In the future, improvement should be focused on the economical and intensive use of ecological land, technical innovations, and the optimal scale. The present results can provide references for making scientific decisions regarding the efficient utilisation of ecological land in the Yangtze Delta and other similar rapid-urbanization areas.
研究领域:
环境科学
·
城市规划
·
系统建模
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可持续发展
·
土地利用管理
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DOI
高影响因子
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
·
2024
·
IF 4.7
Jiang-long YI,Han-lin LIAO,Cheng CHANG,Xing-chen YAN,Min LIU,Ke-song ZHOU
A series of nano-WC surface modified AlSi10Mg powders with different WC contentswere synthesized by the electrostatic assembly method. After the selective laser melting (SLM) processing, various Al−W intermetallicswere in-situ formed in the SLM nano-WC/AlSi10Mg composites. A well-coherent Al/Al 5 W interface with a low lattice mismatch of 4.7% was confirmed with an orientation relationship of (002) α (Al) //(104) Al5W . The nano-WC particles and Al−W intermetallics triggeredthe columnar-to-equiaxed transition, leading to a fine equiaxed microstructure. As a result, the SLMed 3 wt.% nano-WC/AlSi10Mg composites showed remarkable mechanical properties, with an ultimate tensile strength of (464.1±8.68) MPa and elongation of (5.6±0.95)%. Additionally, the SLMed 3 wt.% WC/AlSi10Mg samples also achieved the lowest average coefficient of friction (0.429) and wear rate of 3.842×10 −5 mm 3 /(N·m) compared to other WC/AlSi10Mg composites. The remarkable mechanical and wear properties are ascribed to fine-grain and second-phase strengthening.
研究领域:
材料科学
·
增材制造
·
金属基复合材料
·
力学性能
·
表面工程
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 25
·期 7
·6300-6307
·
IF 2.741
Zhang Li,Liu Jutao,Zhang Dawen,Luo Linguang,Liao Qiegen,Yuan Lijuan,Wu Naicheng
Poyang Lake is the largest freshwater lake in China and an important drinking water source. Since the year 2000, toxic cyanobacteria have been observed frequently in Poyang Lake. In the present study, spatial and seasonal variations of microcystins (MCs; MC-RR, MC-YR, and MC-LR) in water column were examined monthly from January to December (except the months of March, May, and November) in 2013, by using ultra-high-performance liquid chromatography-electrospray ionization tandem triple quadrupole/mass spectrometry (UPLC-MS/MS). MC-RR was the most dominant variant, followed by MC-LR, while MC-YR was detected in low concentration. Total MC concentrations (intracellular + extracellular MCs) ranged from 1.26 to 9916.05 ng/L, with an average of 469.99 ng/L, and only 3.14% (6 out of 192 samples) of the water samples contained MC concentrations that exceeded the drinking water guideline level of 1 μg/L for MC-LR proposed by the World Health Organization (WHO). MC concentrations in water column showed obvious seasonal variations in Poyang Lake. Intracellular and extracellular MCs were both at a low level from January to April but increased quickly from June to August and decreased dramatically thereafter. Intracellular MCs exhibited similar spatial distribution pattern with extracellular MCs. Both intracellular and extracellular MC concentrations in eastern bays and around Songmen Mountain of Poyang Lake were higher than other regions. Intracellular MC concentrations were positively correlated with Chl a ( r = 046, P < 0.01), pH ( r = 0.25, P < 0.01), cyanobacterial biomass ( r = 0.40, P < 0.01), and temperature ( r = 0.36, P < 0.01) but negatively correlated with TN ( r = − 0.28, P < 0.01), suggesting that TN, cyanobacteria biomass, pH, and temperature might be regulating factors for MC production in Poyang Lake.
研究领域:
环境科学
·
湖泊生态学
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水生毒理学
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环境监测
·
淡水生态学
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DOI
高影响因子
危险品化学品经营许可证（带存储）