RSC Advances
·
2015
·Vol. 5
·期 99
·81482-81491
·
IF 3.84
Xuefei Leng,Zhiyong Wei,Yingying Ren,Yang Li,Yurong Wang,Qinyi Wang
In this work, two series of linear-comb and star-comb well defined graft poly(L-lactide) (PLLA) have been synthesized conveniently by one-pot ring-opening polymerization (ROP) of L-lactide using functionalized polybutadiene macroinitiators. The used organocatalyst of 1,8-diazabicyclo[5,4,0]undec-7-ene (DBU) allows the polymerization of L-lactide to proceed rapidly at room temperature. Kinetic studies of the ROP reaction in this system indicate first-order kinetics in monomer concentration. 1H NMR and GPC techniques are employed to characterize the synthesized polymers, validating the formation of the desired comb structures with controllable chain length. Linear-comb and star-comb graft PLLA were comparatively studied as well as with linear PLLA by DSC and POM. The results reveal that the comb structure gives a remarkable improvement of PLLA crystallization ability in both crystallinity and growth rate of spherulites. Furthermore, the more compacted star-comb structure imposes restriction on chain mobility, which weakens the growth effect to some extent. It is found that the glass transition temperature (Tg) and melting temperature (Tm) significantly depend on the side chain length and backbone structure. Rheological studies of both melt and instinct viscosity of the solution show that star-comb PLLA has the lowest hydrodynamic volume compared with linear-comb PLLA and linear PLLA.
研究领域:
高分子化学
·
材料科学
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PHYTOMEDICINE
·
2026
·
IF 11.3
Yuanyuan Liu,Ruoyan Wu,Huijuan Li,Danfeng Pu,Xingang Wang,Shan Chen,Chenjie Jiang,Wangmin Hu,Liyun Wang,Xinhua Ye,Wenlong Huang,Xueqin Wang,Yun Hu,Shiqin Yuan,Yumeng Han,Jun Ye,Qing He,Lan Xu,Yuling Liu
Background Polycystic ovary syndrome (PCOS) is a neuroendocrine-metabolic disorder with no approved drugs. Hypothalamic-pituitary-ovarian (HPO) axis dysfunction is core pathogenesis, and metabolic heterogeneity complicates treatment. Existing medications rarely ameliorate PCOS comprehensively. Sangzhi alkaloids (SZ-A), an extract from Morus alba L. twigs approved for type 2 diabetes in China, have not been systematically explored for PCOS. Purpose To clarify the therapeutic effect and the mechanisms of SZ-A in PCOS. Study Design Animal studies, in vitro experiments and a placebo-controlled clinical trial. Materials and methods In vivo : Obese/non-obese PCOS rats received oral SZ-A 67, 100, 150 mg/(kg·d) for 21 days (positive controls: Diane-35, metformin). In vitro : Granulosa cell apoptosis induced by dihydrotestosterone was treated with SZ-A 25, 50, 100, 200 μg/mL. Clinical: 6-month placebo-controlled trial (128 PCOS patients; oral SZ-A 300 mg/d, n=88; placebo, n=40). Multi-omics/Western blotting explored HPO pathways. Results and discussion In rats, SZ-A (67 to 150 mg/kg/d, 21 days) dose-dependently reduced androgens, regularized estrous cycles, and improved ovarian morphology. In vitro, SZ-A (25 to 200 μg/mL) protected granulosa cells from apoptosis. In patients, 6-month SZ-A (300 mg/d) lowered free androgen index and improved menstrual regularity versus placebo. Mechanistically, SZ-A inhibited hyperandrogenism-induced granulosa cell apoptosis and regulated GnRH secretion and downstream signaling pathways. Conclusion SZ-A exerts therapeutic effects on PCOS by modulating HPO axis function through multiple pathways. Trial registration ChiCTR2200065097
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JOURNAL OF BIOMATERIALS APPLICATIONS
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2026
·
IF 2.4
Zishan Zhou,Hongquan Tao,Haocheng Yang,Ao Duan,Jiahui Yu,Yixin Chen,Yongyan Zhu,Quanhong Zhu
Favorable biocompatibility is essential to biomaterials, and natural amino acids are recognized as the promising building block of polymers due to their non-toxicity and tunable side chains. We prepared polymeric nanoparticles (NPs) using N-acryloyl-L-tryptophan monomer by precipitation polymerization, and modified with polyethylene glycol and folate (PEG-FA) to improve the solubility and target folate-receptors (FR) overexpressed tumor tissues. Serving as drug carriers for vinblastine (VBL), NPs-PEG-FA with about 212.4 nm had the drug loading of VBL of 6.65 ± 0.41% after co-incubating for 1 h and showed sustained-release in pH 7.4 PBS, in which 99.87 ± 1.00% of VBL was released from NPs-PEG-FA during 72 h. Furthermore, NPs-PEG-FA was more efficiently taken up by FR positive Hela cells compared with NPs-PEG, which signified folate could enhance the internalization of NPs-PEG-FA into FR over-expressed cells. And NPs-PEG-FA began to enter Hela cells in large quantities from 3 h onwards, meanwhile the released drug increased more quickly in the first 3 h, which indicated most of the drugs would be released after entering tumor cells. More importantly, NPs-PEG-FA had good biocompatibility to L929 mouse fibroblast cells and exhibited hemocompatibility via the assays of hemolysis, antithrombogenicity, coagulation activation and platelet activation. NPs-PEG-FA could serve as drug carriers for delivering drugs into FR positive tumor cells.
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高影响因子
Journal of Sustainable Agriculture and Environment
·
2026
·Vol. 5
·期 2
·e70172
·
IF 4
Tu Nguyen Quang Le,Karen Robertson,Ian Fisk,Nam Nghiep Tran,Dike Yang,Cordelia Selomulya,Volker Hessel
Phosphorus (P) availability is strongly constrained in both acidic and calcareous soils due to fixation reactions, reducing nutrient-use efficiency and driving excessive fertiliser inputs. This study develops and evaluates a series of chitosan–apatite–citrate ( CAC) fertilisers prepared using four continuous-flow manufacturing strategies: a coiled-flow inverter ( CFI_CAC ), a water/oil sheath-flow system ( Sheath_CAC ), a Stolichem scalable agitated baffle reactor ( Stoli_CAC ), and a pilot-scale spray dryer ( Spray_CAC ). All fertilisers were characterised for composition (phosphate, citrate, chitosan) and microstructure, and their phosphorus releasing behaviours in water were quantified. Distinct release behaviours were observed, which were impacted via citrate content, particle morphology, and the presence of a chitosan hydrogel shell. The Sheath_CAC formulation exhibited the most suitable release profile in aqueous solutions according to the chosen logistic growth model for releasing behaviour ( R 2 = 0.97 ) due to its defined capsule architecture. Radish growth experiments were conducted over two consecutive model seasons in acidic and calcareous soils to assess the functional performance of the materials. In the context of this study, Sheath_CAC consistently produced the high germination rate, leaf development, biomass accumulation, and P uptake across both soils. In acidic soil, Sheath_CAC increased dried root yield by up to 60% and total P uptake by 46.9% compared with the control, while maintaining moderate soil P levels, indicating reduced susceptibility to leaching compared with commercial soluble fertiliser. In calcareous soil, Sheath_CAC partially mitigated P fixation effects, sustaining higher biomass and P uptake relative to the untreated control. While commercial fertiliser maintained the highest available P levels, Sheath_CAC demonstrated stable and controlled release which boosts the yield of radish. These findings highlight Sheath_CAC as a high-performance fertiliser balancing steady P availability with sustained plant growth.
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高影响因子
Gels
·
2026
·Vol. 12
·期 5
·381
·
IF 6.4
Jizhong Yuan,Yaohuang Jiang,Mengle Liu,Peipei Wu,Guoxian Feng,Yanchun Yu,Xiongfa Yang
UV-curable L(-)–borneol-functionalized antibacterial hydrogels for packaging fresh-cut banana and cherry tomato (UV-LBs) were designed from L(-)–borneol-functionalized polyurethane acrylate prepolymers (LB-PUAs) and thiol-functionalized PVA (PVA-SH) using a thiol-ene click reaction initiated by UV light. UV-LBs exhibit unique properties, including excellent thermal stability, high mechanical performance and quite high antibacterial efficiency. The initial thermal decomposition temperature (Td5), tensile strength and elongation at break are in the range of 225–240 °C, 1.38–2.05 MPa and 44.4–68.6%, respectively. The antibacterial efficiency of UV-LBs againstStaphylococcus aureus(S. aureus),Escherichia coli(E. coli), andMonilia albican(M. albican) can reach 67.4%, 75.6% and 83.7%, respectively. The storage time of packaged fresh-cut banana and cherry tomato can be extended from 12 h to 30 h and 4 d to 5 d, respectively.
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GENETIC RESOURCES AND CROP EVOLUTION
·
2013
·Vol. 60
·期 8
·2207-2217
·
IF 1.593
Mikel Mark A.
A major objective of this work is to elucidate the ancestry and genetic background of contemporary U.S. garden pea ( Pisum sativum L. convar. medullare Alef.) cultivars used as cultivated vegetables. This is facilitated through pedigree analysis of 147 cultivars registered during the era from 1990 through 2010 by collecting data from U.S. Plant Variety Protection, U.S. utility patent, and journal publication. Proprietary breeding programs developed 141 cultivars and public programs six, with over half (82 of 147 cultivars) originating from the two (Seminis Vegetable Seeds, Inc. and Syngenta Seeds, Inc.) largest proprietary breeding programs. During this era, the semi-leafless (afila) trait was bred into cultivars to decrease lodging, and 43 % of the cultivars were afila. Registered cultivars were 86 % of determinate growth habit. Bi-parental breeding crosses were used in the development of 78 % of these cultivars. The lineages of the cultivars ‘Bolero’, ‘Spring’, ‘Genie’, and ‘Rally’ made large contributions to the genetic composition of this germplasm, ‘Bolero’ contributed 6.5 % (29 progeny), ‘Spring’ 6.4 % (29), ‘Genie’ 4.0 % (18), and ‘Rally’ 3.6 % (23) of the genes. The garden pea ancestor cultivar ‘Perfection’ fostered the most descendants (75) among the 147 cultivars of this era.
研究领域:
植物学
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遗传学
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植物育种
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园艺学
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作物科学
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高影响因子
RUSSIAN JOURNAL OF ELECTROCHEMISTRY
·
2014
·Vol. 50
·期 11
·1065-1071
·
IF 0.66
Chao Mingyong,Ma Xinying
Herein, a poly( L -tryptophan) modified glassy carbon electrode (Ptry/GCE) for the determination of maltol is fabricated by electrochemical polymerisation. The electrochemical behaviour of maltol at the Ptry/GCE is studied by cyclic voltammetry (CV). The modified electrode shows excellent electrocatalytic activity towards the oxidation of maltol and the oxidation is a one-proton-one-electron process. In pH 8.0 phosphate buffer solution (PBS), the oxidation peak current of maltol shows a linear relationship with its concentration in the range from 9.00 × 10 −5 to 3.75 × 10 −3 mol L −1 with a correlation coefficient of 0.9972. The limit of detection is estimated to be 8.00 × 10 −6 mol L −1 . The novel method shows good selectivity, recovery, reproducibility and great convenience and has been satisfactorily demonstrated in real food sample analysis.
研究领域:
电分析化学
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食品安全
·
传感器与生物传感器
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高影响因子
Antioxidants
·
2023
·Vol. 12
·期 2
·468
·
IF 7
Yanbei Wu,Yali Wang,Zhengyan Liu,Jing Wang
3-deoxyanthocyanidins (3-DAs) are typical flavonoids found in sorghum bran, and they have received much attention in recent years owing to their bioactivities. To further enhance the resource utilization of sorghums cultivated in China, three brewing sorghum cultivars (Liaoza-48, Liaonuo-11, and Liaonian-3) commonly used in China were selected as raw materials for the construction of an extraction technology system for 3-DAs and the clarification of their structures and bioactivities. Based on single-factor experiments and response surface analysis, the optimized system for the extraction of 3-DAs from sorghum grains was as follows: a hydrochloric acid-methanol solution (1:100,v/v) was the extraction solvent, the solid-liquid ratio was 1:20 (g/mL), the extraction time was 130 min, and the temperature was 40 °C. This extraction system was simple and feasible. High performance liquid chromatography analysis suggested that the main monomeric compounds of the extracted 3-DAs were Apigeninidin (AE) and Luteolinidin (LE). Among the three selected sorghum grains, Liaoza-48 had the highest amount of AE (329.64 μg/g) and LE (97.18 μg/g). Antioxidant experiments indicated that the 3-DAs extracted from Liaoza-48 showed higher free-radical scavenging activities for DPPH, ABTS+, and hydroxyl radicals than those extracted from Liaonuo-11 and Liaonian-3. These results provide basic data and technical support for the high-value and comprehensive utilization of sorghums in China.Keywords:sorghum;3-deoxyanthocyanidins;solvent extraction;Apigeninidin;Luteolinidin;bioactivity
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高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2022
·
IF 3.521
Yu Zhang,Dekun Cheng,Zhangtie Wang,Yiwen Guo,Ming Chang,Ruijie Liu
Excessive ultraviolet (UV) exposure is the primary environmental factor that contributes to skin aging. Orbitides have been considered as important functional components in linseed, and they are complex and multiple. In this study, linseed orbitides (LOs) were divided into oxidized linseed orbitides (OLOs) and reduced linseed orbitides (RLOs) and used to investigate protection against ultraviolet B-induced photoaging in zebrafish. First, the results of the zebrafish embryo acute toxicity test showed that the OLOs had obvious embryo toxicity compared with RLOs. And RLOs had better effect in ultraviolet B-treated zebrafish, which may significantly reduce the reactive oxygen species levels and inhibit the degradation of collagen. Besides, we also found that RLOs could effectively inhibit the oxidation of proteins and lipids and regulate the activity of antioxidant enzymes. Furthermore, neutrophil recruitment to the dorsal and caudal fin regions was observed in UVB-treated zebrafish, and RLOs effectively alleviated this migration. In conclusion, RLOs have strong photoprotective effects and potential to be used as antiphotoaging ingredients.
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JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Shuang Liang,Guanting He,Guozhe Sui,Jinlong Li,Dongxuan Guo,Ze Luo,Rongping Xu,Hong Yao,Chao Wang,Zhiyong Xing
Metal organic frameworks (MOFs) are considered as desirable precursors for the preparation of photocatalyst to improve photocatalytic hydrogen evolution efficiency due to the maintained special morphology. Herein, C-doped rod-like ZnO with porous structure has been successfully prepared by using ZIF-L as precursor via an optimized two-step calcination strategy, which is applied for photocatalytic hydrogen evolution reaction from water splitting. The results show that ZnO-350-450 exhibits the best photocatalytic activity with a hydrogen production rate of 0.35 mmol·g −1 ·h −1 , which is 1.9 times higher than that of ZnO-400 (0.184 mmol·g −1 ·h −1 ) under simulated sunlight illumination. The enhanced photocatalytic performance is attributed to the fact that the two-step calcination provides the regular porous rod-like structure (ZIF-L) and the inclusion of carbon in ZnO photocatalyst. C-doped ZnO with regular morphology offers faster charge transfer rate and higher charge separation efficiency, and the C-doped expands a wider range of light absorption. Overall, the presented work could provide a new idea for designing and preparing of photocatalysts with high efficiency for photocatalytic hydrogen production.
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Microbial Biotechnology
·
2022
·Vol. 15
·期 5
·1610-1621
·
IF 6.575
Xing Liu,Xiao Han,Yuan Peng,Chunlin Tan,Jing Wang,Hongsong Xue,Ping Xu,Fei Tao
Summary 3, 4-Dihydroxyphenyl- l -alanine ( l -DOPA) is a compound of high medical value and is considered effective as a treatment for Parkinson’s disease. Currently, bioproduction of l -DOPA is mainly carried out by whole-cell catalysis mediated by recombinant Escherichia coli carrying heterogeneous tyrosine phenol lyase. Vibrio natriegens is increasingly attracting attention owing to its superiority, including extremely rapid growth and high soluble protein expression capacity. In this study, we attempt to develop an efficient whole-cell catalyst for l -DOPA production using V. natriegens as the chassis. The maximum soluble protein expression by V. natriegens was accomplished in 4 h at 37°C, which was equivalent to that achieved by E. coli in 16 h at 16°C. Furthermore, the maximum productivity reached over 10.0 g l −1 h −1 in the early stage of biocatalysis, nearly two-fold higher than previously reported. Approximately 54.0 g l −1 l -DOPA was obtained with a catechol conversion rate greater than 95%. In conclusion, V. natriegens displays advantages, including rapid protein expression and catalytic rate in the catalysis process for l -DOPA production. These findings strongly suggest that V. natriegens has remarkable potential as a whole-cell catalysis chassis for the production of valuable chemicals.
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Frontiers in Plant Science
·
2022
·
IF 6.627
Zhao Lu,Song Zhongbang,Wang Bingwu,Gao Yulong,Shi Junli,Sui Xueyi,Chen Xuejun,Zhang Yihan,Li Yongping
Proanthocyanidins (PAs) are important phenolic compounds and PA biosynthesis is regulated by a ternary MBW complex consisting of a R2R3-MYB regulator, a bHLH factor and a WDR protein. In this study, a tobacco R2R3-MYB factor NtMYB330 was characterized as the PA-specific regulator in which the PA biosynthesis was promoted in the flowers of NtMYB330-overexpressing lines while decreased in the flowers of ntmyb330 mutants. NtMYB330 can interact with flavonoid-related bHLH partner NtAn1b and WDR protein NtAn11-1, and the NtMYB330-NtAn1b complex is required to achieve strong transcriptional activation of the PA-related structural genes NtDFR1, NtANS1, NtLAR1 and NtANR1. Our data reveal that NtMYB330 regulates PA biosynthesis in seeds and affects seed germination, in which NtMYB330-overexpressing lines showed higher PA accumulations in seed coats and inhibited germination, while ntmyb330 mutants had reduced seed coat PAs and improved germination. NtMYB330 affects seed germination possibly through two mechanisms: modulating seed coat PAs to affect coat-imposed dormancy. In addition, NtMYB330 regulates the expressions of abscisic acid (ABA) and gibberellin acid (GA) signaling-related genes, affecting ABA-GA crosstalk and seed germination. This study reveals that NtMYB330 specifically regulates PA biosynthesis via formation of the MBW complex in tobacco flowers and affects germination through adjustment of PA concentrations and ABA/GA signaling in tobacco seeds.
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高影响因子
危险品化学品经营许可证（带存储）