INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Fei-yue Xu,Jin-lin Cai,Bo-ru Chen,Xin-an Zeng,Lang-hong Wang,Rui Wang
This study developed a bilayer emulsion gel using complexes of succinylated whey protein isolate (SWPI) and chitosan (CS) (termed HCS), primarily characterizing its structure, stability, and digestion properties, with curcumin delivery as a model application. Molecular docking revealed succinylation increased SWPI-CS binding sites and affinity improvement. The HCS emulsions exhibited pH/ionic-dependent stability, achieving optimal performance at pH 4 and 160 mM NaCl. Fluorescence imaging validated a trilaminar interface (outer CS layer/inner SWPI layer/oil core). Subsequent experiments incorporating curcumin demonstrated oxidative protection (90 % curcumin retention at 24 h) and digestion resistance (37.5 % bioaccessibility). Rheology demonstrated gelation (G′ > G″) at φ ≥ 0.4, with NaCl strengthening the network. In vitro digestion showed HCS-E gastric resistance (pepsin shielding by CS) and controlled intestinal release (2-fold slower FFA hydrolysis vs. single-layer emulsions). The bilayer design synergistically combines SWPI's emulsification with CS's steric hindrance, offering a platform for bioactive delivery.
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Aquaculture Reports
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2025
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IF 3.7
Mingzuo Gao,Jingu Shi,Zike Yan,Hongyao Zhang,Kaijie Lin,Yutian Zhan,Yuhan Li,Shuyu Han,Jingzhen Liang
This study investigated the effects of dietary curcumin supplementation at different concentrations on the growth performance, intestinal morphology and microbiota, and liver transcriptome of Mauremys mutica . Healthy turtles were randomly assigned to five groups: a control group (C0, 0 mg/kg curcumin) and four treatment groups (C100, C200, C400, and C800) receiving curcumin at 100, 200, 400, and 800 mg/kg diet, respectively. Each group consisted of three replicates (30 turtles per replicate), and the trial lasted for 60 days. The results demonstrated that dietary supplementation with 100, 200, and 400 mg/kg curcumin significantly improved both weight gain rate (WGR) and specific growth rate (SGR), with the 200 mg/kg group showing the highest activities of α-amylase, trypsin, and lipase compared to the control group. Curcumin supplementation also significantly increased intestinal villus height, muscular thickness, and goblet cell counts per villus, indicating an improvement in intestinal morphology. Furthermore, curcumin modulated the gut microbiota composition by increasing the relative abundance of Prevotellaceae_unclassified while decreasing Desulfobacterota, Proteobacteria, Edwardsiella , Bacteroides , and Negativibacillus . Transcriptomic analysis of liver tissues revealed 215, 422, 265, and 243 unique DEGs in the 100, 200, 400, and 800 mg/kg groups, respectively. These DEGs were primarily enriched in immune-related pathways such as the PI3K-Akt, and TNF signaling pathways, as well as metabolism-related pathways including the PPAR signaling pathway, nucleotide metabolism, and various amino acid metabolism pathways. Collectively, these findings indicate that curcumin enhances growth performance and digestive capacity, improves intestinal morphology and digestive enzyme activity, modulates microbial community, and regulates hepatic gene expression related to immune and metabolic function in M. mutica . Based on regression analysis (R 2 > 0.5) of key indicators—including WGR, SGR, FCR, and the activities of trypsin and lipase, as well as malondialdehyde (MDA) content—and with no significant effect on survival, the optimal dietary inclusion level of curcumin for M. mutica was estimated to be between 199.74–266.73 mg/kg.
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International Journal of Nanomedicine
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2025
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IF 6.5
Shuiling Jin,Aifang Zhou,Qi Zhang,Di Zhao,Xintong Dong,Hong-Min Meng,Zhengquan Yu,Zhaohui Li
Purpose Irinotecan (CPT-11), a semisynthetic camptothecin derivative and topoisomerase I inhibitor, is globally used for treating cancers such as colorectal cancer. However, its clinical application is limited by severe adverse effects, including diarrhea and myelosuppression. Biomimetic nano-drug delivery is widely used and can improve chemotherapeutic activity and decrease side effects.Methods In this study, we developed a cancer cell membrane-functionalized metal-organic frameworks for irinotecan/curcumin (named MZCC) for colorectal cancer treatment. In this study, by combining irinotecan, curcumin, MOFs and functionalized cancer cell membrane, a pH-sensitive drug delivery system of MZCC was developed for colorectal cancer treatment with enhanced chemotherapeutic efficacy while reducing side effects. The characteristics and stability of MZCC were carefully examined, and its anti-cancer efficiency was studied in vitro and in vivo. In vivo experiments in a murine colorectal cancer mouse model given intratumorally MZCC injection every 4 days were used to assess antitumor efficacy and systemic toxicity.Results The MZCC system improved treatment antitumor efficacy by 2.3-fold compared with free CPT-11 through synergistic chemotherapy. MZCC-treated groups exhibited a significant reduction in inflammatory cytokine levels within colon tissues; inflammation levels decreased by 55% compared with free CPT-11, and colon length was restored to 79% of normal.Conclusion Our strategy effectively alleviates CPT-11-induced intestinal toxicity and mitigates adverse reactions. This approach may provide a novel method for enhancing chemotherapeutic efficacy while reducing gastrointestinal toxicity.
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FOOD CHEMISTRY
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2025
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IF 9.8
Haifeng Wang,Ting Fang,Ying Chen,Jiang Mou,Teng Li,Yuying Fu,Yun Zhai
This study systematically investigates the structure-function relationships between Maillard-glycated (PWM) and genipin-mediated crosslinked (PWG) pectin-whey protein isolate (WPI) complexes, with particular emphasis on their differential impacts on bioadhesive performance and digestion profiles for curcumin-loaded delivery systems. Both crosslinking strategies induced structural unfolding of WPI and endogenous pectin proteins, exposing amino-active sites that facilitated their reconstitution into stable complexes. PWG developed denser and more homogeneous hydrophobic microdomain networks compared to PWM. Curcumin was encapsulated via hydrogen bonding and hydrophobic interactions within these networks. Ex vivo intestinal adhesion assays demonstrated PWG loaded curcumin (PWG-Cur) achieved 28.69 % particulate retention in colonic environments, representing 7.0-fold and 1.75-fold enhancements over free curcumin and PWM loaded curcumin (PWM-Cur), respectively. In vitro digestion models confirmed PWG-Cur's superior bioaccessibility (22.46 %), reflecting a 37 % enhancement over PWM-Cur (16.35 %). These findings provide critical design principles for engineering mucoadhesive pectin-polyphenol delivery platforms capable of precisely modulating cargo release while enhancing bioaccessibility.
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JOURNAL OF NEUROSCIENCE RESEARCH
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2025
·Vol. 103
·期 7
·e70064
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IF 3.4
Sun Hong,Liu GuoYan,Wu JiaWen,Yu Xin,Zhan ShuQin,Reng HongWei,Chen MengYing,Cheng YuXuan
Chronic epilepsy is mainly characterized by spontaneous recurrent seizures (SRS). The peroxisome proliferator activated receptor gamma/phosphatase and tensin homolog/protein kinase B (PPARγ/PTEN/Akt) pathway is involved in the pathogenesis of SRS and neuronal loss. Curcumin is a natural compound, and previous studies have shown it provides neuroprotection via anti-inflammation and anti-oxidant effects in many central nervous system (CNS) diseases. In the present study, we show that curcumin regulates the abnormal expression of PTEN and Akt in the SRS phase, improves the neuronal loss in the hippocampus, and suppresses SRS development and seizure spike activity in epileptic rats. More importantly, these effects are reversed by the PPARγ antagonist, T0070907, suggesting that curcumin exerts neuroprotective and anti-epileptic effects through the PPARγ/PTEN/Akt signaling pathway. Other studies have shown that curcumin can cross the BBB and has a safety profiles and pleiotropic pharmacological effects. Thus, our data support the proposition that curcumin might be a potential neuroprotective and anti-epileptic agent for chronic epilepsy.
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Rongyi Ye,Ran Yi,Yihan Wang,Nan Huang,Yanhua Wang,Chen Chen
Pesticide toxicity to honeybees has become a pressing ecological issue, yet the effects of pesticide co-exposure are still not fully understood. This research investigates the toxicological implications of concurrent exposure to broflanilide (BFL), a novel diamide insecticide, and myclobutanil (MYC), a commonly used triazole fungicide, on honeybees ( Apis mellifera L . ), while exploring potential preventive strategies. Acute toxicity tests revealed a significantly lower 96-hour lethal concentration 50 (LC 50 ) for BFL (0.34 mg a.i. L −1 ) compared to MYC (82.3 mg a.i. L −1 ), and their co-exposure resulted in pronounced synergistic toxicity. Worker bees were exposed to environmentally relevant doses of BFL and MYC for 7 days, and midgut toxicity was assessed. The co-exposure caused severe midgut damage, including G-layer deterioration, loss of columnar epithelium integrity, and downregulation of the tight junction protein Z O-2. Additionally, oxidative stress-related genes ( Sod1 , Catalase , SelK , GstD1 ) were upregulated, accompanied by higher MDA levels and increased CAT and SOD activities. Furthermore, a greater number of TUNEL-positive cells were detected, along with elevated expression of apoptosis-related genes ( Caspase-3-like , Caspase-8-like , Caspase-9-like ) and higher caspase enzyme activities. Curcumin (Cur) was tested for its protective effects, and it significantly alleviated midgut damage, oxidative stress, and apoptosis. This study reveals the synergistic ecotoxicological effects of pesticide combinations and suggests Cur as a potential prevention strategy for mitigating their harmful impact on honeybees.
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MOLECULAR NEUROBIOLOGY
·
2024
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IF 4.6
Xu Yao,Liu Yongsheng,Wu Yan,Sun Jingshan,Lu Xiaocheng,Dai Kun,Zhang Yiting,Luo Chengliang,Zhang Jian
Early brain injury caused by subarachnoid hemorrhage (SAH) is associated with inflammatory response and ferroptosis. Curcumin alleviates neuroinflammation and oxidative stress by as yet unknown neuroprotective mechanisms. The objective of this study was to investigate the impact of curcumin on neuronal ferroptosis and microglia-induced neuroinflammation following SAH. By examining Nrf2/HO-1 expression levels and ferroptosis biomarkers expression both in vitro and in vivo, it was demonstrated that curcumin effectively suppressed ferroptosis in neurons after SAH through modulation of the Nrf2/HO-1 signaling pathway. Furthermore, by analyzing the expression levels of Nrf2, HO-1, p-p65, and inflammation-related genes, it was confirmed that curcumin could prevent the upregulation of pro-inflammatory factors following SAH by regulating the Nrf2/HO-1/NF-κB signaling pathway in microglia. The ability of curcumin to reduce neuronal damage and cerebral edemas after SAH in mice was validated using TUNEL staining, Nissl staining, and measurement of brain tissue water content. Additionally, through implementation of the modified Garcia test, open field test, and Y-maze test, it was established that curcumin ameliorated neurobehavioral impairments in mice post-SAH. Taken together, these data suggest that curcumin may offer a promising therapeutic approach for improving outcomes following SAH by concurrently attenuating neuronal ferroptosis and reducing neuroinflammation. Graphical Abstract
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2019
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IF 4.527
Sihong Li,Ishfaq Muhammad,Hongxiao Yu,Xiaoqi Sun,Xiuying Zhang
In this study, we identified AFB1 adducts as potential markers and investigated the role of curcumin in alleviating AFB1-induced liver damage by suppressing the production of AFB1 adducts and oxidative stress in AA broilers liver. A total of 64 one-day-old Arbor Acres (AA) broilers were randomly divided into four groups, including control group, AFB1 group (5 mg/kg AFB1), cur + AFB1 group (300 mg/kg curcumin+5 mg/kg AFB1) and curcumin group (300 mg/kg). Serum biochemical parameters, liver antioxidant abilities, AFB1 adducts and oxidative stress mechanism were studied in broilers. AFB1 administration accompany with signs of liver injury, including hepatic histological lesions, increased serum enzymes activities , decreased liver antioxidant enzymes activities and the suppression of ROS and 8-OHdG. Meanwhile, Nrf2/HO-1 pathway was depressed by AFB1 treatment. Immunohistochemistry and ELISA showed that AFB1 significantly increased AFB1-DNA adduct in liver ( p < 0.05) and AFB1-lysine adduct in serum ( p < 0.05). Importantly, supplementation of curcumin can ameliorate these alterations. Intriguingly, curcumin alleviated AFB1-induced toxicity and oxidative stress by inhibiting the generation of ROS, 8-OHdG and AFB1 adducts, and activated Nrf2 signaling pathway in broilers. Conclusively, our experiments suggest that curcumin could be considered as a potential agent for prevention of AFB1-induced toxicity and oxidative stress, and AFB1 adducts could be suitable therapeutic targets.
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FOOD CHEMISTRY
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2025
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IF 9.8
Yuelong Xu,Yu Zhang,Jingcheng Ma,Ting Wang,Chong Zhang
This study elucidates the mechanism of curcumin encapsulation in soy protein isolate (SPI) modified via enzymatic deamidation and pH-shifting. A novel Chryseobacterium sediminis -derived glutaminase (CS-PG) demonstrated superior thermal stability, catalytic efficiency, and specificity over commercial CP-PG. Controlled deamidation by both enzymes enhanced SPI functionality (solubility/emulsification/foaming) with minimal hydrolysis, where CS-PG induced hydrophobic self-assembly and CP-PG promoted hydrophilic domain formation. These distinct conformations dictated binding modes: CS-PG-SPI achieved 92.97 % higher encapsulation efficiency versus SPI through hydrophobic encapsulation, while attained an 85.00 % increase via surface-layer stabilization. pH-shifting further altered SPI conformation by inducing reversible unfolding and refolding, enabling formation of amorphous, stable protein–curcumin complexes. Dual modification significantly enhanced bioaccessibility versus free curcumin—1034 % for CS-SPI–Cur–pH and 1058 % for CP-SPI–Cur–pH. The work establishes enzymatic deamidation as a precision tool for tailoring SPI self-assembly and bioactive delivery in functional foods.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Ya-Long Zheng,Zhi-Shan Tu,Hong-Mei Cui,Shuai Yan,De-Chen Duan,Wei Tang,Fang Dai,Bo Zhou
Reprograming of energy metabolism is a major hallmark of cancer, but its effective intervention is still a challenging task due to metabolic heterogeneity and plasticity of cancer cells. Herein, we report a general redox-based strategy for meeting the challenge. The strategy was exemplified by a dietary curcumin analogue (MitoCur-1) that was designed to target mitochondria (MitoCur-1). By virtue of its electrophilic and mitochondrial-targeting properties, MitoCur-1 generated reactive oxygen species (ROS) more effectively and selectively in HepG2 cells than in L02 cells via the inhibition of mitochondrial antioxidative thioredoxin reductase 2 (TrxR2). The ROS generation preferentially mediated the energy crisis of HepG2 cells in a dual-inhibition fashion against both mitochondrial and glycolytic metabolisms, which could hit the metabolic plasticity of HepG2 cells. The ROS-dependent energy crisis also allowed its preferential killing of HepG2 cells (IC50 = 1.4 μM) over L02 cells (IC50 = 9.1 μM), via induction of cell-cycle arrest, apoptosis and autophagic death, and its high antitumor efficacy in vivo, in nude mice bearing HepG2 tumors (15 mg/kg). These results highlight that inhibiting mitochondrial TrxR2 to produce ROS by electrophiles is a promising redox-based strategy for the effective intervention of cancer cell energy metabolic reprograming.
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Analytical Cellular Pathology
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2021
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IF 2.916
Teng Zhang,Xiaomin Chen,Yueqing Qu,Yanbing Ding
Oxidative stress-induced neuronal damage is a main cause of ischemia/reperfusion injury. Curcumin (Cur), the principal constituent extracted from dried rhizomes of Curcuma longa L. (turmeric), exhibits excellent antioxidant effects. Previous studies have indicated that miR-1287-5p was downregulated in patients with ischemic stroke. Additionally, we predicted that Lon Peptidase 2, Peroxisomal (LONP2), which is involved in oxidative stress regulation, is targeted by miR-1287-5p. The aim of the current study is to investigate the effect of Cur on ischemia/reperfusion damage and its underlying mechanism. To mimic ischemia/reperfusion damage environment, SH-SY5Y cells were subjected to oxygen-glucose-deprivation/reperfusion (OGD/R). OGD/R treatment downregulated miR-1287-5p and upregulated LONP2 in SH-SY5Y cells, but Cur alleviated OGD/R-induced oxidative damage and reversed the effect of OGD/R on the expression of miR-1287-5p and LONP2. Furthermore, we confirmed the interactive relationship between miR-1287-5p and LONP2 (negative regulation). We revealed that miR-1287-5p overexpression alleviated OGD/R-induced oxidative damage alleviation, similar to the effect of Cur. MiR-1287-5p inhibition accentuated OGD/R-induced oxidative damage in SH-SY5Y cells, which was reversed by Cur. The expression of LONP2 in OGD/R-treated SH-SY5Y cells was decreased by miR-1287-5p overexpression and increased by miR-1287-5p inhibition, and Cur counteracted the increase in LONP2 expression induced by miR-1287-5p inhibition. In conclusion, we suggest that Cur alleviates OGD/R-induced oxidative damage in SH-SY5Y cells by regulating the miR-1287-5p/LONP2 axis. The findings provide a theoretical basis for the clinical application of curcumin.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Yan Hu,Shangwen Zhang,Zhijie Wen,Hudie Fu,Jie Hu,Xuexin Ye,Li Kang,Xiaojun Li,Xinzhou Yang
Anti-inflammatory drugs for ulcerative colitis (UC) treatment should specifically penetrate and accumulate in the colon tissue. Herein, a multi-bioresponsive anti-inflammatory drug (curcumin, CUR)-loaded heterogeneous double-membrane microgels ( [email protected] ) for oral administration was fabricated in this study, in which the inner core was derived from polyvinyl alcohol (PVA) and guar gum (GG) and the outer gel was decoration with alginate and chitosan by polyelectrolyte interactions. The structure and morphology of microgels were characterized. In vitro, the formulation exhibited good bio-responses at different pH conditions and sustained-release properties in simulated colon fluid with a drug-release rate of 84.6 % over 34 h. With the assistance of the outlayer gels, the microgels effectively delayed the premature drug release of CUR in the upper gastrointestinal tract. In vivo studies revealed that [email protected] specifically accumulated in the colon tissue for 24 h, which suggest that the interlayer gels were apt to reach colon lesion. As expected, the oral administration of microgels remarkably alleviated the symptoms of UC and protected the colon tissue in DSS-induced UC mice. The above results indicated that these facilely fabricated microgels which exhibited excellent biocompatibility and multi-bioresponsive drug release, had an apparent effect on the treatment of UC, which represents a promising drug delivery strategy for CUR in a clinical application.
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