Molecular Medicine Reports
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2024
·Vol. 29
·期 5
·1-11
·
IF 3.4
Jiayan Yang,Liangliang Dong,Yifan Wang,Lifen Gong,Hongwei Gao,Yicheng Xie
Acute lung injury (ALI) is an acute inflammatory lung disease associated with both innate and adaptive immune responses. Hexokinase 2 (HK2) is specifically highly expressed in numerous types of inflammation‑related diseases and models. In the present study in vitro and in vivo effects of targeted degradation of HK2 on ALI were explored. The degradation of HK2 by the targeting peptide TAT (transactivator of transcription protein of HIV‑1)‑ataxin 1 (ATXN1)‑chaperone‑mediated autophagy‑targeting motif (CTM) was demonstrated by ELISA and western blotting in vitro and in vivo. The inhibitory effects of TAT‑ATXN1‑CTM on lipopolysaccharide (LPS)‑induced inflammatory responses were examined using ELISAs. The therapeutic effects of TAT‑ATXN1‑CTM on LPS‑induced ALI were examined via histological examination and ELISAs in mice. 10 µM TAT‑ATXN1‑CTM administration decreased HK2 protein expression and the secretion of proinflammatory cytokines (TNF‑α and IL‑1β) without altering HK2 mRNA expression in LPS‑treated both in vitro and in vivo, while pathological lung tissue damage and the accumulation of leukocytes, neutrophils, macrophages and lymphocytes in ALI were also significantly suppressed by 10 µM TAT‑ATXN1‑CTM treatment. TAT‑ATXN1‑CTM exhibited anti‑inflammatory activity in vitro and decreased the severity of ALI in vivo. HK2 degradation may represent a novel therapeutic approach for ALI.
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IONICS
·
2024
·
IF 2.4
Yan Qiang,Zhu Wenxu,Ma Xiaoxue,Liu Na,Liu Yucheng,Xia Meichen
A series of ether-functionalized paramagnetic ionic liquids, 1-(2-methoxyethyl)-3-alkylimidazolium tetra thiocyanate cobalt [C n 2O1IM] 2 [Co(NCS) 4 ] ( n = 1, 2, 3), was synthesized and characterized. The density, surface tension, refractive index, and electrical conductivity of these ionic liquids were measured at 293.15 to 343.15 K at intervals of 5 K, and their thermal expansion coefficients α were calculated. The molecular volume was obtained by measuring the pore volume and porosity. Based on Glasser theory, the standard entropy S 0 (298 K), lattice energy U POT , surface entropy S a , and surface enthalpy, H of the ionic liquids were calculated, and the reason why the ionic liquid is in a molten state at room temperature was explained from the perspective of lattice energy. The molar surface Gibbs energy was introduced to improve the traditional Eötvös equation, which was combined with the refractive index to estimate the surface tension, obtaining a fitting index exceeding 0.99. Finally, the relationship between the electrical conductivity of the ionic liquids and temperature was investigated, and the activation energy, molar electrical conductivity, and electrical conductivity diffusion coefficient of the ionic liquids were obtained. The relationship between their properties was summarized. Compared with previously reported ionic liquids using 1-(2-methoxyethyl)-3-methylimidazolium as a cation, this type of ionic liquid has higher density and a smaller coefficient of thermal expansion.
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ChemCatChem
·
2024
·
IF 3.8
Siyang Yan,Ruilin Feng,Jiaxu Liu,Chenxi Bai,Yanlong Qi
Graphical The dehydration of 2,4-pentanediol to unsaturated alcohol was performed over ceria-based catalysts with high selectivity. A stereocatalytic effect of CeO 2 catalyst was observed for the production of trans -3-penten-2-ol, with negligible content of cis -3-penten-2-ol. The transformation of biobased alkanediols over heterogeneous catalysts is a major object. Here, a series of catalysts were prepared for 2,4-pentanediol dehydration to 3-penten-2-ol. Ceria-based catalysts had a unique property of showing high efficiency. More importantly, a stereocatalytic effect was observed for CeO 2 , yielding trans -3-penten-2-ol with a high selectivity. Raman, XPS, TPD, and TPR were carried out to clarify the catalyst structure, and the relationship between the catalyst structure and catalytic performance were studied. The amount of Lewis acid sites can hardly be correlated with the total concentrations of the oxygen vacancies. It was suggested that the geometry structure of the active sites played a crucial role in trans -3-penten-2-ol production. The oxygen vacancies originating from CeO 2 consisted of active sites with a geometrical structure, which were different from those created by Pr doping CeO 2 . In addition, the Pr doping enhanced the oxygen migration, which resulted in side reactions, consequently, giving a lower selectivity to trans -3-penten-2-ol.
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2024
·
IF 4.2
Fangfang Ding,Huimei Wang,Yingzhi Li,Xueping Leng,Jiaming Gao,Danfei Huang
Due to the massive production and use of plastic, the chronic and evolving exposure to microplastics in our daily lives is omnipresent. Nonylphenol (NP), a persistent organic pollutant, may change toxicity when it co-exists with microplastics. In this study, polystyrene microplastics (PS-MPs), either alone or with pre-absorbed NP, generated oxidative stress and inflammatory lesions to Caco-2 cells, as well as affecting proliferation via the MAPK signaling pathway and causing apoptosis. Damage to cell membrane integrity and intestinal barrier (marked by lower transepithelial electric resistance, greater bypass transport, and tight junction structural changes) leads to enhanced internalization risk of PS-MPs. Some important intestinal functions including nutrient absorption and xenobiotic protection were also harmed. It is worth noting that the exposure of PS-MPs with a diameter of 0.1 μm improved intestinal functions quickly but acted as a chemosensitizer for a long time, inhibiting cell perception of other toxic substances and making the cells more vulnerable.
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INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Han Luo,Yu Zhang,Tingyu Wang,Ling Huang,Liling Cao,Xuehua Dong,Guohong Zou
Two antimony selenites, Sb2O2SeO3 and Sb2O(SeO3)2, were synthesized by simultaneously incorporating stereochemically active lone pair electrons containing SeO32– and Sb3+. These compounds are structured with [SbOx] polyhedra and [SeO3] units within a two-dimensional framework. Both of them exhibit cutoffs at 300 and 330 nm within the ultraviolet (UV) range and demonstrate significant birefringence, with indices of 0.069 and 0.126 at 546 nm, respectively. These properties highlight their potential as UV birefringent materials. Structural analyses and theoretical calculations reveal that their exceptional birefringence results from the synergistic interactions between SeO32– anions and Sb3+ cations.
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Materials Chemistry Frontiers
·
2025
·
IF 6.4
Xiyue Zhang,Yingjie Zhou,Minjie Hou,Xiecheng Yang,Kun Ren,Peng Dong,Jiaqian Qin,Da Zhang,Feng Liang
To meet the urgent requirement for sustainable energy storage technologies, incorporating effective waste management into the design of energy storage materials can achieve a closed-loop supply chain from waste to energy. Herein, extrinsic pseudocapacitive (PDC) cathode materials with different carbon contents were synthesized using FeSO4·7H2O waste through a simple ball milling and annealing process, which enhances sodium storage kinetics and achieves waste recycling. The results indicate that NFSO particles and graphite flakes create a disordered porous structure, which enhances the PDC effect, thus overcoming the slow migration rate of sodium-ions. Specifically, the diffusion coefficient of NFSO/C-10 increased by around an order of magnitude compared to that of NFSO, increasing from 10−12 to 10−11. The discharge capacity reached 84 mA h g−1 at 0.2C (1C = 91 mA g−1). The present work realizes the high-value reuse of waste and highlights the crucial role of the PDC effect in enhancing diffusion dynamics, which opens up a new direction for the design and synthesis of high-rate iron-based sulfate cathode materials.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jing Tang,Guangrong Zou,Chen Chen,Jing Ren,Fang Wang,Zilin Chen
The identification of formylpyrimidines in DNA is crucial for a better understanding of epigenetics. Although many techniques have been explored to detect their content, more accurate methods of formylpyrimidine determination are still required due to the relatively lower sensitivity or lack of selectivity in current methods. Herein, an electrochemical method based on the covalent bonding of the azido derivative of (2-benzimidazolyl) acetonitrile (azi-BIAN) and the aldehyde group of 5-formyluracil (5fU) was proposed for the selective detection of 5fU in the presence of 5-formylcytosine (5fC) and apyrimidinic (AP) sites. Target DNA containing 5fU was first treated with azi-BIAN and then incubated with DBCO-PEG4-Biotin to introduce a biotin group by copper-free click chemistry. Next, the sulfhydryl group was attached to the 5′ end of above DNA through T4 polynucleotide kinase-catalyzed reaction. Subsequently, the labeled DNA was assembled onto the AuNPs-modified glassy carbon electrode (AuNPs/GCE) through Au–S bonds, and the streptavidin–horseradish peroxidase conjugate (SA-HRP) was further immobilized onto the surface of the above electrode by specific recognition between biotin and streptavidin. Finally, HRP catalyzed hydroquinone oxidation to benzoquinone to enhance the current signal, which was related to the amount of 5fU in nucleic acids. This method demonstrated a good linear relationship with 5fU concentrations ranging from 0.1 to 10 nM. Moreover, the level of 5fU in γ-irradiated nucleic acids was also successfully detected, indicating that the combination of molecule-depended chemical recognition and electrochemical sensing is a promising method for the selective and sensitive detection of 5fU.
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Journal of Biomedical Nanotechnology
·
2021
·Vol. 17
·期 8
·1545-1553
·
IF 4.099
Xiao Chuanguang,Wang Xiaohong,Shen Jiacheng,Xia Yanjie,Qiu Shusheng,Liang Xiaodong,Gu Wenyue
Despite the broad anticancer activity, whereas the clinical application of luteolin is hindered by unsatisfactory water solubility and non-targeting. Herein, targeted inhibitory effects of luteolin-loading HER2 nanospheres (Her-2-NPs) were successfully prepared by thin film ultrasonic
method. In comparison with the non-targeted nanospheres, Her-2 nanospheres could significantly boost the intake of luteolin in SK-BR-3 cells. The proliferation and apoptosis of breast cancer cells were detected by MTT testing and flow cytometry examination, respectively. Consequently, the
expressions of FOXO1 mRNA level was detected using qPCR assay and protein level was detected using Westernblot. We discovered that Luteolin-loading Her-2 nanospheres could significantly hinder the proliferation of breast cancer cells, down-regulation their migration, and up-regulation FOXO1
expression at mRNA and protein levels, reveal a mechanism whereby luteolin interferes with breast cancer. Collectively, these results suggest Her-2-modified nanospheres increases the efficiency of luteolin uptake and thus improves the treatment benefit of breast cancer.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ting Chen,Pengfei Liu,Huanxiang Wang,Yue Su,Sheng Li,Shimeng Ma,Xuan Xu,Jie Wen,Zhen Zou
Accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is of great importance to control the COVID-19 pandemic. The gold standard assays for COVID-19 diagnostics are mainly based on separately detecting open reading frame 1ab (ORF1ab) and nucleoprotein (N) genes by RT-PCR. However, the current approaches often obtain false positive-misdiagnose caused by cross-contamination or undesired amplification. To address this issue, herein, we proposed a dumbbell-type triplex molecular switch (DTMS)-based, logic-gated strategy for high-fidelity SARS-CoV-2 RNA detection. The DTMS consists of a triple-helical stem region and two-loop regions for recognizing the ORF1ab and N genes of SARS-CoV-2. Only when the ORF1ab and N gene are concurrent, DTMS experiences a structural rearrangement, thus, bringing the two pyrenes into spacer proximity and leading to a new signal readout. This strategy allows detecting SARS-CoV-2 RNA with a detection limit of 1.3 nM, independent of nucleic acid amplification, holding great potential as an indicator probe for screening of COVID-19 and other population-wide epidemics.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Wei Song,Kai Zheng,Xin Xu,Cong Gao,Liang Guo,Jia Liu,Xiulai Chen,Liming Liu,Guipeng Hu,Jing Wu
l-Ascorbic acid-2-phosphate (AsA-2P) is stable in aqueous solution and at high temperatures and is widely used in foods, pharmaceuticals, cosmetics, and fodders; however, practical application of enzymatic synthesis methods to promote industrial-scale production of AsA-2P remains a major challenge. In this study, we enhanced the phosphorylation efficiency of Pseudomonas aeruginosa acid phosphatase (APase; EC 3.1.3.2) for AsA-2P production via protein engineering. Among the mutants obtained, we selected the most efficient mutant (Var5; G125E/D135T/S136N), which exhibited an increased kcat of 18.6 s–1 and a Km for AsA of 223.9 mM. In addition, Var5 exhibited a maximum enzyme activity of 2080.4 U/L after 10 h of fermentation, which was 80% higher than the wild-type enzyme. Furthermore, under optimal conditions, Var5 showed a maximal conversion of 48.6% and achieved a final AsA-2P titer of 61.5 g/L at 8 h, which is considerably higher than that reported for other similar biocatalytic approaches. These findings demonstrate the potential of this method for the large-scale production of AsA-2P.
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BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
·
2021
·
IF 1.632
Yingxue Liao,Zhijian Ni,Jinyong Wu,Zhongkui Li,Yuanfei Ge,Xiangsong Chen,Jianming Yao
2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2'-FL production. The highest 2'-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the arcA mutant strain using shake-flask culture. In addition, the arcA mutant showed a significantly reduced accumulation of acetate. However, the deletion of iclR and the double deletion of arcA/iclR provided no benefit in terms of 2'-FL biosynthesis. Overall, these results suggest that deletion of arcA could efficiently increase the 2'-FL yield in E. coli and could lay a foundation for more efficient and larger-scale production. Moreover, in shake-flask culture under optimal conditions, the 2'-FL titer reached a maximum value of 7.14 g/L.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Hui Xu,Ye Hu,Qiuhui Hu,Jianhui Liu,Anxiang Su,Minhao Xie,Gaoxing Ma,Fei Pei,Alfred Mugambi Mariga,Wenjian Yang
Flammulina velutipes has anti-inflammatory, immunomodulatory, antioxidant and many bioactive properties with high contents of carbohydrate, proteins and fibers. In this study, a novel proteoglycan with polysaccharide complexes and protein chain, named PGD1-1, was isolated from F. velutipes . The structural characteristics of PGD1-1 were then determined, and its anti-proliferation and pro-apoptotic activities against HepG-2 cells were demonstrated in vitro. Results proved that the average molecular weight of PGD1-1 was 32.71 kDa, and the carbohydrate and protein contents were 93.35 and 2.33%, respectively. The protein moiety was bonded to a polysaccharide chain via O-glycosidic linkage. The monosaccharides consisted of d -glucose, D-galactose and D-xylose in a molar ratio of 21.90:2.84:1.00. PGD1-1 significantly inhibited the proliferation of HepG-2 cells by affecting cell lipid peroxidation and nitric oxide production. In addition, PGD1-1 promoted the apoptosis of HepG-2 cells, especially the early apoptosis. These findings proved that PGD1-1 was a novel potent ingredient against the proliferation of HepG-2, which will provide a theoretical basis for the development and utilization of the functional ingredients of the F. velutipes .
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