PEPTIDES
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2018
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IF 2.851
Fengjing Jia,Yi Zhang,Jiayi Wang,Jinxiu Peng,Ping Zhao,Lishi Zhang,Haiyan Yao,Jingman Ni,Kairong Wang
Antimicrobial peptides (AMPs) are believed to be a promising class of antimicrobial agents against bacteria and fungi. To promote the clinical use of AMPs, their antimicrobial activity and susceptibility to protease degradation should be further improved. The antimicrobial peptide Jelleine-I was originally isolated from the royal jelly of honeybees ( Apis mellifera ) with a short sequence of PFKLSLHL-NH 2 (953.24 Da). Here, a series of halogenated derivatives of the antimicrobial peptide Jelleine-I were designed and synthesized. The results showed that the in vitro antimicrobial activity, antibiofilm activity and in vivo antimicrobial efficacy were enhanced 1-8-fold after halogenation. Additionally, the proteolytic stability of Jelleine-I was improved 10-100-fold by halogenation. Meanwhile, the halogenated derivatives retained negligible hemolytic activity and cytotoxicity. Among these derivatives, the antimicrobial activity and antibiofilm activity of chlorine-Jelleine-I (Cl-J-I), bromine-Jelleine-I (Br-J-I), and iodine-Jelleine-I (I-J-I) were better than those of fluorine-Jelleine-I (F-J-I). The stabilities of Br-J-I and I-J-I against the degradation of enzymes and the serum were better than those of F-J-I and Cl-J-I. In conclusion, this study may offer a useful strategy to enhance antimicrobial efficacy and proteolytic stability by halogenation. The halogenated derivatives Cl-J-I, Br-J-I and I-J-I may be considered as potential antimicrobial agents against microbial infection.
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ACS Applied Materials & Interfaces
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2018
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IF 8.097
Yu-Xia Li,Yu-Nong Ji,Meng-Meng Jin,Shi-Chao Qi,Shuai-Shuai Li,Ding-Ming Xue,Ming Bo Yue,Xiao-Qin Liu,Lin-Bing Sun
Here, we designed a double-solvent/host–guest redox combined strategy to construct Cu+ sites in metal–organic frameworks (MOFs) for the first time. As a proof of concept, a representative MOF MIL-100(Fe) with tunable valence states of cations was employed as the host. The combined strategy realizes selective introduction of Cu2+ precursors to the interior pores of MIL-100(Fe), remarkably minimizing the aggregation of Cu2+ and subsequently formed Cu+ species. Owing to the proper reducibility of in situ formed Fe2+ in the frameworks, controlled conversation of Cu2+ to Cu+ with ∼100% yield is achieved in the absence of any additional reducing agents. These characteristics make the obtained materials Cu+-modified MIL-100(Fe) highly active in selective CO adsorption. The CO adsorption capacity is up to 3.75 mmol·g–1 at 298 K and 1 bar, which is superior to all other Cu+-containing adsorbents reported so far such as CuCl/activated carbon (2.5 mmol·g–1), CuCl/γ-Al2O3 (1.0 mmol·g–1), and CuCl/SBA-15 (0.50 mmol·g–1). The same adsorbent also exhibits quite high selectivity of CO over N2, and the ideal adsorption solution theory selectivity reaches 424. The outstanding CO adsorption performance make the present adsorbents great potential in separation of CO from various mixtures.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
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IF 3.412
Jingjing Luo,Xiaofeng Meng,Jianyu Su,Hang Ma,Wen Wang,Liming Fang,Huade Zheng,Yexia Qin,Tianfeng Chen
15,16-Dihydrotanshinone I (DI), a natural compound isolated from a traditional Asian functional food Salvia Miltiorrhiza Bunge, is known for its anticancer activity. However, poor solubility of DI limits its desirable anticancer application. Herein, polylactic-co-glycolic acid (PLGA) was functionalized with polyethylene glycol (PEG) and biotin to form copolymers PEG-PLGA (PPA) and biotin-PEG-PLGA (BPA). DI was encapsulated in copolymers PPA and BPA to obtain DI-PPA-NPs (NPs = nanoparticles) and DI-BPA-NPs, respectively. The particle size and its distribution, encapsulation efficiency, and in vitro releasing capacity of DI-BPA-NPs were characterized by biophysical methods. MTT assay was used to evaluate the antiproliferative activity of free DI, DI-PPA-NPs, and DI-BPA-NPs in human cervical cancer Hela cells. DI-BPA-NPs showed the highest cytotoxicity on Hela cells with an IC50 value of 4.55 ± 0.631 μM, while it was 8.20 ± 0.849 and 6.14 ± 0.312 μM for DI and DI-PPA-NPs in 72 h, respectively. The superior antiproliferative activity was supported by the fact that DI-BPA-NPs could be preferentially internalized by Hela cells, owing to their specific interaction between biotin and overexpressed biotin receptors. In addition, DI-BPA-NPs effectively inhibited Hela cell proliferation by inducing G2/M phase cycle arrest and decreasing the intracellular reactive oxygen species (ROS) level by 31.50 ± 2.29% in 5 min. In summary, DI-BPA-NPs shows improved antiproliferative activity against human cervical cancer as comparing with free DI, demonstrating its application potential in cancer therapy.
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SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Jinjie Wang,Shuyi Ma,Jicun Ren,Jinxia Yang,Yi Qu,Derun Ding,Min Zhang,Guang Yang
Keratins are a group of cysteine-rich structural proteins found abundant in hair, wool, feathers, etc. In this work, a highly luminescent gold nanocluster (AuNCs-Ag@Keratin) was synthesized for the first time by using keratin as the template, followed by a simple modification with silver(I) ions. The prepared AuNCs-Ag@Keratin could exhibit dramatically enhanced red fluorescence (λ em = 725 nm), which was about 5-folds higher than that of common AuNCs@Keratin. The interactions of AuNCs with silver(I) ions were systematically characterized by fluorescence measurements, fluorescence correlation spectroscopy (FCS) and X-ray photoelectron spectroscopy (XPS). It was demonstrated that the “silver effect” could not only enhance the fluorescence intensity of AuNCs, but also improved the particles’ dispersion and stability. The as-prepared AuNCs-Ag@Keratin was applied to detect Hg 2+ in a wide detection range (2.44–2500 nM) and low detection limit (2.31 nM).
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 10
·1-6
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IF 5.705
Ma Liang,Guo Ting,Pan Shuli,Zhang Yuhao
A fluorometric patulin (PAT) assay is presented that is based on the use of magnetic reduced graphene oxide (rGO) and DNase I. The fluorescence of the PAT aptamer labelled with 6-carboxyfluorescein (FAM) is quenched by magnetized reduced graphene oxide (rGO-Fe 3 O 4 ) due to fluorescence resonance energy transfer (FRET). However, in the presence of PAT, the labelled aptamer is stripped off from rGO-Fe 3 O 4 . The rGO-Fe 3 O 4 is then magnetically separated so that the fluorescence of free labelled PAT aptamer is restored. DNase I cannot hydrolyze the aptamer on rGO-Fe 3 O 4 , but it can cleave the free aptamer-PAT complex. This will release FAM and PAT which can undergo a number of additional cycles to trigger the cleavage of abundant aptamer. Recycling of DNase I-assisted target therefore leads to a strong amplification of fluorescence and consequently to an assay with low limit of detection. The detection limit for PAT is as low as 0.28 μg L −1 which is about 13 times lower than that without using DNase I. The method offers a new approach towards rapid, sensitive and selective detection based on an aptamer. Conceivably, it has a wide scope in that it may be applied to numerous other analytes if appropriate aptamers are available.
Schematic of a fluorometric assay based on the use of magnetic graphene oxide and DNase I. It was applied to the determination of patulin. DNase I was introduced for recycling amplification. The detection limit is about 13 times lower than that without using DNase I. Figure a contains poor quality of text in image. Otherwise, please provide replacement figure file.Thank you. I will provide the figure file.
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CHEMISTRY OF MATERIALS
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2018
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IF 9.89
Ying Liu,Fei Li,Quanlin Liu,Zhiguo Xia
All inorganic cesium lead halide CsPbX3 (X = Cl, Br, I) perovskite nanocrystals (NCs) are generally considered to be sensitive and even decompose when exposed to ambient humidity. Herein, we perform a well-controlled investigation of the effect of postsynthetic water treatment of the as-prepared CsPbX3 NCs on the photoluminescence quantum yields (PLQYs) and stability, and one can find that the surface defective layer of CsPbX3 NCs can be dissolved into water and we can further separate the supernatant CsPbX3 NCs hexane solution from the water, which improves the PLQYs of CsPbCl3, CsPbBr3, CsPbI3 from 5%, 69.3%, 68.1% to 78.5%, 95.5%, 91%, respectively. As a synergetic effect, a trace amount of residual water facilitated the hydrolysis of tetramethyl orthosilicate, leading to the formation of SiO2-coated CsPbX3 NCs with high stability. The stable green-emitting CsPbBr3/SiO2 composite is used as an example to combine with red K2SiF6:Mn4+ to fabricate the light-emitting diodes device on the blue InGaN chip, which displays wide color gamut (128% of National Television Standard Committee standard), providing the possibility for liquid-crystal display backlight utilization.
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Frontiers in Plant Science
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2017
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IF 4.291
Chen Guilin,Guo Mingquan
Topoisomerase I (Topo I) catalyzes topological interconversion of duplex DNA during DNA replication and transcription, and has been deemed as important antineoplastic targets. In this study, the fraction R.d-60 from ethyl acetate extracts of Rhamnus davurica showed higher inhibitory rates against SGC-7901 and HT-29 compared with the R.d-30 fraction in vitro. However, the specific active components of R.d-60 fraction remain elusive. To this end, a method based on bio-affinity ultrafiltration and high performance liquid chromatography/electrospray mass spectrometry (HPLC- ESI-MS/MS) was developed to rapidly screen and identify the Topo I inhibitors in this fraction. The enrichment factors (EFs) were calculated to evaluate the binding affinities between the bioactive constituents and Topo I. As a result, eight ligands were identified and six of which with higher EFs showed more potential antitumor activity. Furthermore, antiproliferative assays in vitro (IC50 values) with two representative candidates (apigenin, quercetin) against SGC-7901, HT-29 and Hep G2 cells were conducted and further validated. Finally, the structure-activity relationships revealed that flavones contain a C2-C3 double bond of C ring exhibited higher bio-affinities to Topo I than those without it. This integrated method combining Topo I ultrafiltration with HPLC-MS/MS proved to be very efficient in rapid screening and identification of potential Topo I inhibitors from the complex extracts of medicinal plants, and could be further explored as a valuable high-throughput screening platform in the early drug discovery stage.
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ACS Applied Nano Materials
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2024
·
IF 5.3
Yu Yao,Qianhong Shen,Qifeng Chen,Yanyan He,Libei Jiang,Jie Liu,Yue Li,Xudan Cao,Hui Yang
A silver phosphate (Ag3PO4)/silver (Ag)/silver halide (AgX, X = Cl, Br, or I) ternary composite photocatalysts were prepared by loading Ag nanoparticles on Ag3PO4 tetrapods followed with in situ halogenation. The interface charge transfer behavior in the Ag3PO4/Ag/AgX nanocomposite was studied, and the photocatalytic performance was also evaluated. The results show that the Ag3PO4/Ag/AgI photocatalyst exhibits higher photocatalytic activity than the other two photocatalysts due to the more matched band structure between AgI and Ag3PO4. An internal electric field (IEF) from Ag3PO4 to AgI is formed at the heterogeneous interface, so the photogenerated electrons at the conduction band of AgI can rapidly transfer to the valence band of Ag3PO4 and recombine with photogenerated holes, which is consistent with the S-scheme charge transfer mechanism. Meanwhile, the photogenerated electrons left at the conduction band of Ag3PO4 can transfer to Ag nanoparticles due to the Schottky junction and react with oxygen to produce •O2–, which is proved to be the main active species in the photocatalytic procedure. This tandem junction modulated between the S-scheme heterojunction and Schottky junction promotes the efficient separation of photogenerated carriers, thus significantly increasing the average PL lifetime of electrons by 2.95 times. Therefore, the Ag3PO4/Ag/AgI photocatalyst exhibits excellent photocatalytic activity for the degradation of methylene blue and norfloxacin. The seven consecutive cyclic tests prove the good stability of the photocatalyst, thus showing the application potential in the field of sewage treatment.
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DRUGS IN R&D
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2024
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IF 2.2
Jia Caiyun,Zhao Na,Song Haojing,Hu Yiting,Xu Yufang,Guo Caihui,Bai Wanjun,Dong Zhanjun
Background Ondansetron is a highly selective 5-HT3 receptor antagonist that alleviates nausea and vomiting. Bioequivalence evaluation ensures that the efficacy of generic drugs is consistent with that of the original drug. Objective The objective of this study was to evaluate the bioequivalence of ondansetron hydrochloride (HCl) tablets taken in single doses under fasting and postprandial conditions in healthy subjects. Methods In this randomized, open-label, two-cycle, crossover phase I study, liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) was used to determine the ondansetron concentration in dipotassium-ethylenediaminetetraacetate (K 2 -EDTA) plasma after the subjects received a single 8 mg of ondansetron and reference formulation. Twenty-six healthy subjects received one tablet of ondansetron under fasting conditions and 28 subjects received one under postprandial conditions. Bioequivalence was established if the 90% confidence interval (CI) was 80.00–125.00%. The pharmacokinetic parameters were calculated via WinNonLin 8.1 software and the bioequivalence data were evaluated via Phoenix WinNonlin 8.1 statistics software. Results The geometric mean ratio (GMR) of the maximum observed concentration ( C max ), the area under the plasma concentration‒time curve (AUC) from time zero to the last sampling time (AUC 0– t ), and the AUC from time zero to infinity (AUC 0–∞ ) from the test/reference formulation under fasting conditions were 90.50, 90.43, and 90.25, respectively. The 90% CIs were 83.75–97.79, 82.64–98.95, and 82.25–99.03, respectively. The GMRs of C max , AUC 0– t , and AUC 0–∞ after a high-fat meal were 96.85, 93.57, and 93.77, respectively; the 90% CIs were 88.43–106.07, 87.35–100.24, and 87.35–100.68, respectively. Conclusion The test and reference formulations of ondansetron HCl have bioequivalence for healthy adult subjects under fasting and postprandial conditions.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2015
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IF 2.729
Meiling Wang,Zhengnian Chen,Yaomin Chen,Chunjin Zhan,Jianwei Zhao
Positively charged gold nanoparticles (AuNPs) were self-assembled onto the surfaces of 1-allyl-3-methylimidazolium chloride (AMIM-Cl) functionalized reduced graphene oxide sheets (RGO) here. The AMIM-Cl functionalized RGO (ILRGO) loaded with AuNPs (ILRGO@AuNPs) modified glass carbon electrodes (GCEs) possess larger effective specific surface areas which can favor the adsorption of more Sudan I into the working electrode . So the ILRGO@AuNPs composites were successfully used for the fabrication of a facile and sensitive sensor for the determination of Sudan I. And the prepared sensor exhibits a broad linear range for Sudan I spanned 4 orders of magnitude from 1.0 × 10 − 10 to 1.0 × 10 − 6 M and a detection limit as low as 5.0 × 10 − 11 M (S/N = 3). It is expected that this green sensor can be applied in the determination of other similar dyes.
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TALANTA
·
2017
·
IF 4.162
Lan Luo,Fengzhou Xu,Hui Shi,Xiaoxiao He,Taiping Qing,Yanli Lei,Jinlu Tang,Dinggeng He,Kemin Wang
Deoxyribonuclease I (DNase I) is an important physiological indicator and diagnostic biomarker, but traditional methods for assessing its activity are time-consuming, laborious, and usually radioactive. Herein, by effectively combining the special functions of DNase I and terminal deoxynucleotidyl transferase (TdT), a simple, green, cost-effective, label-free and ultrasensitive assay for DNase I activity has been constructed based on superlong poly(thymine)-hosted copper nanoparticles (poly T-CuNPs). In this strategy, a 3′-phosphorylated DNA primer is designed to block TdT polymerization. After addition of DNase I, the primer could be digested to release 3′-hydroxylated fragments, which could further be tailed by TdT in dTTP pool with superlong poly T ssDNA for CuNPs formation. Fluorescence measurements and gel electrophoresis demonstrated its feasibility for DNase I analysis. The results indicated that with a size of 3–4 nm, the CuNPs templated by TdT-polymerized superlong poly T (>500 mer) had several advantages such as short synthetic time (<5 min), large Stokes shift (~275 nm) and intense red fluorescence emission . Under the optimal conditions, quantitative detection of DNase I was realized, showing a good linear correlation between 0.02 and 2.0 U/mL (R 2 =0.9928) and a detection limit of 0.02 U/mL. By selecting six other nucleases or proteins as controls, an excellent specificity was also verified. Then, the strategy was successfully applied to detect DNase I in diluted serum with a standard addition method, thus implying its reliability and practicability for biological samples. The proposed strategy might be promising as a sensing platform for related molecular biology and disease studies.
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Nanoscale Research Letters
·
2017
·Vol. 12
·期 1
·1-8
·
IF 2.833
Zhang Z. L.,Men B. Q.,Liu Y. F.,Gao H. P.,Mao Y. L.
Precursor solution of CH 3 NH 3 PbI 3-x Cl x for perovskite solar cells was conventionally prepared by mixing PbCl 2 and CH 3 NH 3 I with a mole ratio of 1:3 (PbCl 2 :CH 3 NH 3 I). While in the present study, CH 3 NH 3 PbI 3-x Cl x -based solar cells were fabricated using the precursor solutions containing PbCl 2 and CH 3 NH 3 I with the mole ratios of 1:3, 1.05:3, 1.1:3, and 1.15:3, respectively. The results display that the solar cells with the mole ratio of 1.1:3 present higher power conversion efficiency and less I-V hysteresis than those with the mole ratio of 1:3. Based on some investigations, it is concluded that the higher efficiency could be due to the smooth and pinhole free film formation, high optical absorption, suitable energy band gap, and the large electron transfer efficiency, and the less I-V hysteresis may be attributed to the small low frequency capacitance of the device.
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