Frontiers in Immunology
·
2025
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IF 5.9
Huimin Xie,Lin Ma,Xiaoli He,Songsong Zhao,Jin Wang,Ao Zhu,Changming Liu,Olga Piskareva,Chao Deng,Fenghua Meng,Mi Liu
Introduction: Cancer vaccines work through activating tumor-specific T cells, which can specifically attack cancer cells by recognizing antigens binding with Major-Histocompatibility-Complex I (MHC I) molecules. The downregulation or loss of MHC I expression on tumor cells can affect the efficacy of cancer vaccines.Methods: Herein, to increase the MHC I expression on tumor cells, a nanovesicle-based strategy was developed to improve the efficacy of cancer vaccines. Several clinically applied medicines, such as tyrosine kinase inhibitors (TKIs), were screened for their capacity to upregulate MHC I.Results: Two TKIs, Sunitinib and Sorafenib, were found to be very effective in elevating MHC I expression, and they were encapsulated into redox-responsive nanovesicles respectively (SUN-KD10 or SOR-KD10), which demonstrated favourable tumor-targeting capabilities in the tumor microenvironment. Sunitinib or Sorafenib activates the IFNγ/STAT1 pathway, which improve the expression of MHC I. When combined with whole-tumor-antigen-loaded nanovaccines, these nanovesicle formulations elicited a synergistic antitumor effect in both breast cancer and melanoma mouse models. The tumors in the tumor-bearing mice treated with combined strategy grew more slowly and the survival times of such mice are significantly prolonged.Discussion: The studies demonstrated that more tumor-specific T cells were activated in the combined strategy treated mice, suggesting improved immune-mediated tumor clearance. This combinatorial approach provides a promising strategy to overcome immune evasion and to enhance the therapeutic outcomes of cancer vaccine-based immunotherapy by using clinical-applied medicines with cancer vaccines.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Chengren Ouyang,Jielin Yu,Haixin Teng,Guanrong Ou,Bingru Lu,Xu Zhang,Hao Xie,Chun-Qiong Zhou
The combination of type I photodynamic therapy (PDT) and photothermal therapy (PTT) has attracted increased interest in the clinical treatment of tumors because of the suitability of oxygen-independent therapy for hypoxic tumors and the maintenance of high PTT efficacy by reactive oxygen species (ROS). Particularly, near-infrared (NIR) organic molecules have become a research hotspot because of their good reproducibility and low toxicity. However, the majority of them are still far from meeting the clinical requirements for phototherapeutic efficacy and biosafety. Herein, a croconium derivative, CR-497 , was synthesized with benzo[ e ]indole and (thiophen-2-yl)piperazine and complexed with Fe 3+ ( CR-497 -Fe 3+ ) and a tumor-targeting peptide to construct the CR-497 -Fe 3+ nanoparticle (NP) platform. At the breast tumor site, the released CR-497 exhibited pH activation for NIR fluorescence (NIRF) and photoacoustic (PA) imaging, type I PDT, and PTT. In the tumor microenvironment, where glutathione (GSH) and H 2 O 2 were overexpressed, the accumulated Fe 3+ induced ferroptosis and •OH generation. ROS from chemodynamic therapy (CDT) and PDT and lipid peroxidation from ferroptosis inhibited the expression of heat shock proteins (HSPs) and maintained the high efficacy of PTT. In a mouse model, CR-497 -Fe 3+ NPs accurately located the tumor by NIRF and PA bioimaging and exhibited the combined therapeutic effects of ferroptosis, CDT, PDT and PTT with good biosafety. This study presents an effective strategy for the design of tumor-targeted single-component croconium nanoplatforms that exhibit excellent biosafety and enhanced efficacy for type I PDT and PTT.
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ACS Nano
·
2025
·
IF 16
Hui Wang,Weitao Yang,Yan Xu,Weiwei Zeng,Dinghua Liu,Tianming Cui,Yanjing Yun,Chang Liu,Zhuoyao Wu,Bingbo Zhang
Monovalent copper (Cu(I)) drives cuproptosis and catalytic therapy, yet its rapid oxidation reduces the activity. We developed bioinspired Cu(I)-bovine serum albumin single-atom nanozymes (Cu(I)-BSA SAzymes) via mild biomineralization, achieving stability (>90 days) and dual enzyme-like activities (peroxidase (POD)/catalase (CAT)) to convert tumor H2O2 into cytotoxic reactive oxygen species (ROS) while alleviating hypoxia. These SAzymes deplete glutathione to amplify oxidative stress and release Cu(I), which aggregates dihydrolipoamide S-acetyltransferase (DLAT) to induce cuproptosis─distinct from apoptosis/ferroptosis. Mechanistically, transcriptomics analyses suggest that Cu(I)-BSA SAzymes exerted their effects by regulating the oxidative stress and energy metabolism in 4T1 cells. The tumor microenvironment further mediates SAzymes aggregation and in situ transformation into paramagnetic Cu(II), enabling self-amplified T1-weighted MRI contrast for real-time activation monitoring. In breast (4T1) and colon (CT26) tumors, Cu(I)-BSA SAzymes suppress growth by 68.8% and 80.3% ([Cu] = 4 mg kg–1). This study reports a stable Cu(I) platform for catalytic-plus-cuproptosis theranostics.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Xuedong Jia,Hongyuan Guo,Xiu Zhao,Ruining Liu,Xiayu Song,Zhuo Shi,Yingchun Zhu,Suozhu Wu
A novel electrochemical sensor based on gold nanoparticles-poly(phenylboronic acid)/glass carbon electrode (AuNPs-poly(PBA)/GCE) was successfully constructed for detecting nitrite (NO 2 − ) ions using the iodine/iodide (I 2 / I − ) mixed system as a probe. The AuNPs-poly(PBA) composites were firstly prepared on a bare GCE by one-step electrodeposition. By combining the specific enrichment ability of poly(PBA) to NO 2 − and I 2 / I − with the catalytic activity of AuNPs to the reaction between them, the response range of this sensor towards NO 2 − was 1.0 × 10 −11 M to 1.0 × 10 −4 M with a limit of detection (LOD) of 1.7 × 10 −12 M (S/N = 3). The sensor had the advantages of easy preparation, fast response, wide linear range, low LOD, and good selectivity, and could be applied to detect NO 2 − in beef and tap water samples with satisfactory results, which provided a highly sensitive and rapid analysis approach in the fields of food safety and environmental monitoring.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Xueyi Cai,Ziqiang Lin,Lang Liu,Wangwang Yan,Zi Wang,Yang Zhang,Xiao Chen,Dong Liang
Momordica charantia L., commonly known as bitter gourd, is a popular vegetable and medical herb in many countries. The most characteristic phytochemical profile of bitter gourd is its high content of cucurbitane-type triterpenoids, which are responsible for its bitter taste and therapeutic potential. More than 260 cucurbitane-type triterpenoids, including at least 11 5,19-hemiacetals, 40 5,19-methyl acetals, 46 methyl ethers, 6 5,19-ethyl acetals, and 7 ethyl ethers, have been identified from bitter gourd. In this work, by studying the chemical transformation of momordicine I, one of the most abundant cucurbitane-type triterpenoids in bitter gourd, we showed that under acidic conditions, 5,19-hemiacetals of cucurbitane-type triterpenoids could be formed from the intramolecular nucleophilic addition between the C-19 aldehyde and the C-7 hydroxyl group; furthermore, 5,19-acetals and methyl ethers could be generated if methanol was available. Using a UPLC-MS/MS quantification method, we showed that these momordicine I derivatives could not be detected from bitter gourd fruits when extracted with a nonmethanolic solution but could be detected when extracted with methanol after thermal treatment. Moreover, these momordicine I derivatives were detected in two of eight brands of bitter gourd dietary supplements. This might be concerning because these derivatives were not the natural components of bitter gourd, and their safety and efficacy profiles were unknown. Besides, the transformation reactions reported herein should apply widely to other cucurbitane-type triterpenoids as well. Therefore, we believe all 40 5,19-acetals, 46 methyl ethers, 6 5,19-ethyl acetals, and 7 ethyl ethers were the extraction and isolation artifacts and should not be regarded as the natural components of bitter gourd. This work will help to clarify the current misunderstanding of the real cucurbitane-type triterpenoid profile of bitter gourd.
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REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Lin Yang,Xueling Liu,Bingyue Song,Sixian Yang,Xiaojie Zhang,Chong Zhao,Yixiao Wu,Lei Zhang,Yanjun Huang
Effective and selective recovery of precious metal silver (Ag(I)) from wastewater can promote resource recycling and mitigate environmental pollution. In this study, dimercaptosuccinic acid (DMSA) functionalized polyhedral oligomeric silsesquioxane (POSS)-based hybrid polymer (DPHP) was synthesized and employed for the adsorption of Ag(I) from aqueous solution. The obtained adsorbent was characterized using SEM-EDS, XPS, solid-state 13 C NMR, and 29 Si NMR spectra. Batch adsorption experiments were conducted to evaluate the adsorption performance of DPHP for Ag(I). The results revealed that the maximum adsorption capacity of DPHP for Ag(I) was 494.65 mg g −1 at pH 4, and the adsorption process could be well described by pseudo-second-order and Sips models. Thermodynamic studies implied the adsorption of Ag(I) was an endothermic and spontaneous adsorption process in nature. Adsorption and desorption experiments demonstrated that the regeneration efficiency of DPHP remained above 83 % after five cycles and the preconcentration factor of DPHP was determined to be 125. Furthermore, DPHP exhibited selectivity to Ag(I) in solutions containing a variety of competitive metal ions and simulated industrial wastewater. Based on density functional theory (DFT) calculations, as well as XPS, FTIR, and Raman analyses, the interaction between Ag(I) and DPHP involved electrostatic attraction, ion exchange, and coordination, with the coordination mainly between thioether and carboxy groups and Ag(I) ions. Overall, DPHP is a promising adsorbent for the recovery of Ag(I) from aqueous solutions.
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ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Baoling Tang,Jiance Jin,Kai Han,Tianrui Li,Haoran Zhang,Xuejie Zhang,Maxim Molokeev,Yuzhen Wang,Zhiguo Xia,Bingfu Lei
Exploring new types of scintillators, especially fabricating large-area scintillating screens, is essential in applications of life science, industry, and material science. However, the thickness and light scattering in composite scintillator film present a major challenge for balancing the spatial resolution and radioluminescence intensity. Herein, an in situ crystallization route is developed to innovate the preparation of the large-area scintillating screens based on the hybrid copper(I)-based C 6 H 8 N 2 OCuX (X = I and Br), in which carboxymethylcellulose sodium (CMC-Na) contribute to the “CMC-Na membrane” film formation and further increase absorption cross-section for X-ray radiation owing to the existence of Na elements. The choice of halogen can regulate the photoluminescence of C 6 H 8 N 2 OCuX (X = I, Br, and Cl) from cyan to green and then to yellow, and significant scintillation property can be achieved for C 6 H 8 N 2 OCuI with the light yield of 52 000 photons MeV −1 and detection limit of 43.14 nGy s −1 . Finally, the high X-ray absorption assisted by CMC-Na, as well as the thin thickness of the composite scintillator films help to realize remarkable spatial resolution above 14 lp mm −1 . This study provides a foundation for discovering high-performance copper(I)-based halides scintillators and offers a creative think film preparation method for X-ray imaging.
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RSC Advances
·
2018
·Vol. 8
·期 65
·37451-37461
·
IF 2.936
Limo Zhang,Yunsong Jiang,Zhongtian Yin,Jinyuan Sun,Hehe Li,Xiaotao Sun,Mingquan Huang,Fuping Zheng
In the present study, fermented grains (Jiupei), the raw material for Baijiu distillation, were used to isolate and identify low molecular weight peptides with angiotensin-I-converting enzyme (ACE) inhibitory activities. The methods of peptides extraction from Jiupei are described as follows: ultrasonication, centrifugation and filtration. Peptide purification was performed by ultrafiltration, adsorption on a macroporous resin, gel chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC). HPLC with quadrupole-time-of-flight-mass spectrometry/mass spectrometry (HPLC-Q-TOF-MS/MS) was used to identify the peptides, and the ACE inhibitory activities of the peptides were measured. Finally, the optimum peptide extraction and separation parameters were determined to be a liquid/solid (ultrapure water/Jiupei powder) ratio of 15 mL g−1, extraction temperature of 57 °C and ultrasonication time of 33 min. XAD-16 resin was used for the removal of sugars and salts. Under these conditions, the total yield of peptides extracted was 57.682 mg/1 g Jiupei. The peptides identified were Val-Asn-Pro and Tyr-Gly-Asp. Val-Asn-Pro exhibited some ACE inhibitory activity (IC50 = 38.02 μM), while Tyr-Gly-Asp showed higher ACE inhibitory activity (IC50 = 5.21 μM). These results provide an important foundation for the study of peptides in Jiupei and show a reference for the trace of peptides in Baijiu production.
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ACS Biomaterials Science & Engineering
·
2022
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IF 5.395
Wenyi Xia,Tao Yan,Lianlei Wen,Shijie Zhu,Wang Yin,Miao Zhu,Meidong Lang,Chunsheng Wang,Changfa Guo
Despite the fact that heart transplantation (HTx) is a relatively mature procedure, heart ischemic and reperfusion (I/R) injury during HTx remains a challenge. Even after a successful operation, the heart will be at risk of primary graft failure and mortality during the first year. In this study, temperature-sensitive polymer poly(N-n-propylacrylamide-co-N-tert-butyl acrylamide) (PNNTBA) was coated on diallyl trisulfide (DATS)-loaded mesoporous silica nanoparticles (DATS-MSN) to synthesize hypothermia-triggered hydrogen sulfide (H2S) releasing particles (HT-MSN). Because the PNNTBA shell dissolves in phosphate-buffered saline at 4 °C, the loaded DATS could continuously release H2S within 6 h when activated by glutathione (GSH). Furthermore, after co-culturing biocompatible HT-MSN with cardiomyocytes, H2S released from HT-MSN at 4 °C was found to protect cardiomyocytes from ischemic and reperfusion (I/R) injury. In detail, the rate of cell apoptosis and lactate dehydrogenase activity was decreased, as manifested by increased BCL-2 expression and decreased BAX expression. More importantly, in an isolated heart preservation experiment, HT-MSN demonstrated potent protection against cardiac I/R injury and reduced expression of inflammatory factors TNF-α and IL-1β. This study provided a new method for the controlled release of H2S by the donor and myocardial protection from I/R injury.
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ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Jinyang Chen,Xinghu Ji,Zhike He
On the basis of an interesting experimental phenomenon, a novel and smart composite reagent consisting of double-stranded DNA-templated copper nanoparticles (dsDNA–CuNPs) and DNA intercalator (SYBR Green I) was developed and exploited for hydrogen peroxide (H2O2) detection as well as oxidase-based biosensing. The study found that, within the composite reagent, the small molecule SYBR Green I was easily adsorbed on the surfaces of CuNPs, instead of intercalating into the dsDNA. So the composite reagent only exhibited the red fluorescence generated from dsDNA–CuNPs. However, when the solution of H2O2 was added into the composite reagent, the CuNPs were deconstructed and their fluorescence was quenched; meanwhile, the inhibition of SYBR Green I binding with dsDNA was eliminated. As a result, the mixed solution of the composite reagent with H2O2 exhibited green fluorescence generated from the intercalation of SYBR Green I into dsDNA. Since H2O2 is an important molecule and involved in various research fields, this developed composite reagent could be employed for many applications in biological analysis. As a proof-of-application demonstration, the sensitive detection of glucose was conducted. Moreover, the method was also extended to the detection of other biomolecules, such as cholesterol and horseradish peroxidase, which indicated the broad applications of the proposed sensing strategy in biomedical analysis.
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
·
2014
·
IF 3.679
Sheng-Jie Chen,Yong-Qi Li,Yong-Yong Wang,Xiao-Li Zhao,Ye Liu
The ionic hybrid P,N-ligands of 1 and 2 have been synthesized as one type of unconventional tertiary phosphines with the advantages of both the π-accepting ligands and the hemilabile ligands. The corresponding ionic Rh(I)-complexes of 1A and 2A proved to be more efficient catalysts for hydroformylation of 1-octene over isomerization than the traditional Rh I (acac)(CO)(PPh 3 ), due to the π-backdonation interaction in Rh → P linkage and the hemilabile ligation of N,P-coordinating sites to Rh-center. The in situ high-pressure FT-IR analysis indicated that: (1) the formation and lifetime of the active Rh H species (2047 cm −1 ) derived from 1A are facilitated by the presence of ligand 1 ; (2) the formation of the active Rh H species is depressed without the aid of the P,N-hemilabile ligation; and (3) the active Rh H species derived from Rh I (acac)(CO)(PPh 3 ) is unstable and susceptible to the formation of the inactive carbonyl-bridged Rh-dimer.
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APPLIED OPTICS
·
2016
·Vol. 55
·期 35
·9907-9916
·
IF 1.598
Hongpeng Liu,Dan Yu,Ke Zhou,Dongyao Mao,Langbo Liu,Hui Wang,Weibo Wang,Qinggong Song
Temperature-induced diffraction spectrum responses of holographic
gratings are characterized for exploring the temperature-sensing
capability of a holographic sensor. Linear blue shift of peak
wavelength and linear diffraction reduction are observed. It provides
quantitative expressions for sensing applications. Inorganic
nanoparticles are dispersed into the binder to improve sensing
properties. Obvious improvement of sensing parameters, including
wavelength shift and diffraction change, is confirmed. The
sensitivity, response rate, and linear response region of holographic
sensors are determined to evaluate sensing capacity. Influence of
relative humidity on holographic sensing response is discussed.
Expansion of humidity range provides a probability for extending the
range of wavelength shift. Finally, the temperature response
reversibility of a holographic sensor is evaluated. These experimental
results can expand the practical application field of holographic
sensing strategy and accelerate the development of holographic
sensors.© 2016 Optical Society of
America
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