Cell Death & Disease
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2021
·Vol. 12
·期 6
·1-16
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IF 8.469
Chi Xiaochun,Luo Weiwei,Song Jiagui,Li Bing,Su Tiantian,Yu Miao,Wang Tianzhuo,Wang Zhenbin,Liu Cheng,Li Zhen,He Huiying,Zhan Jun,Zhang Hongquan
Kindlin-2 is known to play important roles in the development of mesoderm-derived tissues including myocardium, smooth muscle, cartilage and blood vessels. However, nothing is known for the role of Kindlin-2 in mesoderm-derived reproductive organs. Here, we report that loss of Kindlin-2 in Sertoli cells caused severe testis hypoplasia, abnormal germ cell development and complete infertility in male mice. Functionally, loss of Kindlin-2 inhibits proliferation, increases apoptosis, impairs phagocytosis in Sertoli cells and destroyed the integration of blood-testis barrier structure in testes. Mechanistically, Kindlin-2 interacts with LATS1 and YAP, the key components of Hippo pathway. Kindlin-2 impedes LATS1 interaction with YAP, and depletion of Kindlin-2 enhances LATS1 interaction with YAP, increases YAP phosphorylation and decreases its nuclear translocation. For clinical relevance, lower Kindlin-2 expression and decreased nucleus localization of YAP was found in SCOS patients. Collectively, we demonstrated that Kindlin-2 in Sertoli cells is essential for sperm development and male reproduction.
研究领域:
生殖生物学
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发育生物学
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分子生物学
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IEEE SENSORS JOURNAL
·
2025
·Vol. 26
·期 2
·1957-1965
·
IF 4.5
Fang Wang,Gan Liu,Binggang Xiao,Jianyuan Qin
Resonances originating from the concept of bound states in the continuum (BIC) have been shown to produce high quality factors and strong field enhancements. Here, an ultrasensitive terahertz (THz) metallic metasurface based on BIC is developed to address the limited sensitivity of THz sensing technology. The metasurface employs a u-shaped resonator and an l-shaped resonator as the unit cell, and a resonance around 0.7 THz can be excited. The analysis of the quality factor and the band structure confirms that this resonance is induced by the BIC mode. Based on the calculations, it can be concluded that the frequency shift changes linearly as a function of analyte thickness within the range of 0–10 μm, and the sensitivity can be as high as 151 GHz/RIU. The feasibility of the detection of chlorpyrifos concentration in soybean oil is assessed experimentally, and a liquid cell is applied as a sample holder to maintain the sample in a liquid state. It can be concluded that the metasurface enables effective detection of chlorpyrifos in soybean oil, with a limit of detection (LOD) as low as 0.007 mg/L. Our work enables the detection of trace chlorpyrifos in soybean oil, facilitating the application of THz technology in food safety.
研究领域:
生物传感器
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食品安全
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光学检测
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分子诊断
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食品科学
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物理化学
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分析化学
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Acta Pharmaceutica Sinica B
·
2026
·
IF 14.6
Jingjing Shi,Junyu Zhang,Xuting Zhou,Dandan Zhang,Hui Qi,Simian Chen,Yunlong Hou,Zhenhua Jia,Caisheng Wu
Comprehensive identification of in vivo metabolites from natural medicines (NMs) remains challenging due to their chemical complexity and the limited reference spectra. Here, we presented the deep mass dataset relevance bridging (Deep MDRB) platform, an integrated platform powered by a diagnostic weighted cosine similarity (DWCS) algorithm that efficiently facilitates high-coverage annotation of in vivo metabolites. By integrating mass spectral databases with biosynthetic and metabolic reaction rules, Deep MDRB enables fully automated non-targeted analysis, including peak extraction, background correction, multimodal data alignment, multilevel similarity matching, and parent–metabolite network bridging. As a proof of concept, Deep MDRB was applied to in vitro liver microsome models of verapamil and berberine, as well as an in vivo metabolic study involving mixed reference standards (MRS) and Tongxinluo (TXL) capsule in rats. The platform identified 1565 parent compounds with a 94.4% identification rate for the TXL capsule and constructed a biotransformation network of 29 in vitro , 83 (MRS) and 2069 (TXL capsule) in vivo metabolite nodes, respectively, with a correlation rate exceeding 90%. Compared to existing software, Deep MDRB substantially improves annotation coverage and network integrity. Overall, the “knowledge-data” dual-driven framework of Deep MDRB provides a novel strategy for metabolite profiling in complex systems.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Xiaobing Wang,Yun Gao,Hanyu Chai,Kai Yang,Jinsu Liao,Chao Zhang,Jianhuan Wei,Fazheng Ren,Pengjie Wang,Yi Wang
Buffalo milk caseins coagulated faster into larger, rougher curds in gastric phase. • Buffalo milk showed slower and more sustained protein hydrolysis in gastric phase. • Fewer peptide species with lower sequence coverage were generated from buffalo milk. • Clot structure during gastric digestion was the key factor affecting hydrolysis. This study compared the digestion behavior and peptide profiles of water buffalo and Holstein skim milk using the standardized INFOGEST in vitro protocol. During the gastric phase, protein hydrolysis and amino acid release demonstrated that buffalo milk proteins underwent a slower but more sustained digestion process than those of Holstein milk. SDS–PAGE analysis further confirmed the slower rate of protein hydrolysis in buffalo milk. Particle size distribution and confocal microscopy revealed that buffalo milk coagulated more rapidly into larger and more wrinkled coagulates than Holstein milk, which likely reduced pepsin accessibility and contributed to the slower protein hydrolysis. LC–MS peptide profiling further showed that buffalo milk generated fewer peptide species with lower protein sequence coverage following gastric digestion. In the intestinal phase, proteins from both milks were rapidly degraded, and species differences in protein hydrolysis behavior and peptide profiles became less pronounced. Download: Download high-res image (165KB) Download: Download full-size image
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Frontiers in Veterinary Science
·
2026
·
IF 3.1
Chengxiang Guo,Hao Ling,Junbing Mao,Jiale Dong,Cai Zhang,Yue Wang,Shuai Wang,Shuai Guo,Jicang Wang
Cadmium (Cd) is a widespread toxicant with high bioaccumulation potential. This study explores the interplay of endoplasmic reticulum stress (ERS), autophagy, and apoptosis in Cd-induced hepatotoxicity, focusing on whether ERS-driven autophagy protects against liver injury. Male SD rats (4 weeks old, n = 24) were acclimatized for 7 days and randomized into four groups receiving CdCl₂ at doses of 0, 0.5, 1, or 2 mg/kg for 14 consecutive days. A second cohort (n = 36) was similarly allocated to six groups: control, Cd, 4-PBA, Cd + 4-PBA, CQ and Cd + CQ. All treatments were administered via daily intraperitoneal injection throughout the study. On day 14, blood and liver tissues were collected for analyses of liver function, hematological parameters, and histopathology. The expression of target factors was analyzed via qRT-PCR and Western blotting. Results indicate that Cd exposure causes liver injury and disordered hepatocyte morphology. ERS markers Grp78 and Caspase-12 and autophagy-related factors Beclin-1, Atg5, P62 and LC3 are upregulated at both mRNA and protein levels. The endoplasmic reticulum-phagy (ER-phagy) receptor FAM134B and the apoptosis effector cleaved Caspase3 are upregulated at the protein level. These findings indicate that Cd induces ERS, UPR activation, autophagy, reticulophagy, and apoptosis. The ERS inhibitor 4-PBA markedly attenuated Cd-induced hepatic injury. Compared with the Cd group, the Cd + 4-PBA group showed decreased serum ALT and AST levels, as well as reduced RBC, WBC, MCH, and MCV counts. At the molecular level, mRNA and protein expression of ER stress markers Grp78, Caspase-12, PERK, eIF2α, ATF4, IRE1α, JNK, and ATF6 were all downregulated. Protein levels of the ER-phagy receptor FAM134B and the apoptosis effector cleaved Caspase-3 were also decreased. The autophagy inhibitor CQ aggravated such injury. Compared with the Cd group, the Cd + CQ group showed increased serum ALT and AST levels, as well as elevated RBC, MCH, and MCV counts. At the protein level, expression of the autophagy-related factors P62 and LC3, and the apoptosis effector cleaved Caspase-3 was significantly increased. These results show that Cd-induced ERS activates autophagy and reticulophagy mainly via the PERK, IRE1α, and ATF6 pathways, and this adaptive response clears autophagic substrates to alleviate hepatocyte damage.
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Pathogens
·
2026
·Vol. 15
·期 8
·860
·
IF 3.8
Qian Wang,Liping Peng,Youjing Wang,Jiajia Hu,Liangying Kong,Yajun Liu
Plant-parasitic nematodes rely on the perception of host-derived chemical cues released from plant roots to locate suitable hosts, representing a critical prerequisite for successful plant infection. In this study, we selected four representative tomato (Solanum lycopersicumL.) root-derived chemical signals and demonstrated their distinct effects on the host-seeking behavior of the southern root-knot nematode (Meloidogyne incognita). Among these compounds, the flavonoids quercetin and luteolin exhibited significant attractant effects, whereas the alkaloids dihydrocapsaicin and solasodine showed repellent effects. RNA interference (RNAi) experiments further indicated that multiple chemosensory neurons are involved in the recognition of plant-derived chemical cues. Silencing of key chemosensory genes significantly impaired the directed chemotactic response ofM. incognitatoward tomato roots and reduced its infectivity, demonstrating that the chemosensory system represents a crucial molecular basis regulating host localization and parasitic establishment. This study provides new insights into host recognition mechanisms in root-knot nematodes and offers a theoretical foundation for developing environmentally sustainable strategies for nematode management by targeting host perception processes.
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2026
·
IF 5.2
Junkang Zhao,Yue Zhang,Jinyu Li,Yuli Zhu,Xunuo Song,Yao Meng,Yuming Zhang,Qinghua Deng,Liyin Du
Chlorpyrifos (CPF), a widely used organophosphate insecticide, can cause intestinal toxicity under chronic exposure conditions, but the upstream mechanisms underlying intestinal epithelial barrier-associated injury remain unclear. This study investigated whether endoplasmic reticulum stress (ERS) contributes to CPF-induced tight junction (TJ) alterations and intestinal epithelial injury. We used an 8-week drinking-water exposure model in weaned ICR mice with 30, 60, and 120 mg/L CPF and a MODE-K cell model, combined with pharmacological ERS modulation using 4-phenylbutyric acid (4-PBA) and tunicamycin (TM). Quantitative single-cell immunofluorescence was performed at 12, 24, and 48 h to assess temporal changes in TJ proteins and apoptosis-related markers. CPF induced jejunal villus atrophy, reduced the villus-to-crypt ratio, downregulated ZO-1, Occludin, and Claudin-1, impaired TJ structural integrity, increased the total protein levels of PERK, IRE1, and ATF6, and enhanced apoptosis-related alterations, including increased Bax and total Caspase-3 levels and decreased Bcl-2 levels. 4-PBA partially restored TJ protein expression and attenuated apoptosis-related alterations, whereas TM further aggravated TJ protein loss and apoptosis-related changes. Time-course analysis showed that TJ protein reductions were detectable before marked apoptosis-related changes and persisted at later time points. Collectively, chronic CPF exposure induced jejunal epithelial barrier-associated injury in weaned mice, accompanied by coordinated increases in the total protein levels of UPR sensors, TJ protein reductions, and enhanced apoptosis-related alterations. The opposing effects of 4-PBA and TM support the involvement of ERS in CPF-associated epithelial injury. These findings suggest that ERS contributes to progressive intestinal epithelial injury induced by CPF.
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JOURNAL OF MEMBRANE SCIENCE
·
2026
·
IF 8.8
Qiankun Wu,Wentian Zhang,Cunxian Lai,Houlong Yang,Fangang Meng,Shanshan Zhao
This study proposes a bio-inspired in-situ cross-linking strategy to address the critical issue of swelling and structural instability in MXene two-dimensional (2D) lamellar membranes. By anchoring gallic acid (GA) onto MXene nanosheets via hydrogen bonding and subsequently coordinating cross-linking with Al 3+ ions, a robust three-dimensional GA-Al network is constructed between the nanosheets. This approach effectively transforms the inherently swellable, loosely stacked MXene assembly into a coordination-crosslinked reinforced lamellar architecture, successfully yielding structurally stable MXene/GA-Al composite membranes with excellent separation performance. The GA-Al network significantly suppressed interlayer swelling (the dry-wet d-spacing difference is only 1.2 Å), enabling precise sub-nanometer channel tuning, mitigating interlayer defects, and improving surface hydrophilicity. The optimized membrane achieved over 99% rejection of Congo red (CR), a pure water permeability of 120.0 L m −2 h −1 bar −1 , and outstanding separation factors of 1105 and 1403 for CR/NaCl and CR/Na 2 SO 4 mixed solutions, respectively. These results outperform most documented 2D lamellar and nanofiltration membranes. This work offers a new design paradigm for developing high-performance, anti-swelling 2D lamellar separation membranes.
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MOLECULES
·
2026
·Vol. 31
·期 12
·1997
·
IF 5.1
Fang Shi,Weibin Jin,Jingchun Wu,Bo Zhao,Chunlong Zhang,Lifeng Mao
To address gas channeling, low sweep efficiency, and water sensitivity in CO2flooding of shale reservoirs, amidine-functionalized graphene quantum dots (FN-GQDs) were synthesized via amidation of citric acid-derived GQDs. FTIR and UV-Vis confirmed successful grafting. Conductometric titration showed an optimal reaction time of 24 h with a grafting ratio of 58%, in good agreement with the 60% saturation predicted by molecular dynamics simulation. Upon CO2introduction, protonation of amidine groups induced a nonlinear viscosity increase from 0.298 to 2.0 mPa·s at 0.02 wt% via electrostatic attraction and hydrogen bonding, forming a dynamic crosslinking network. FN-GQDs maintained low oil-water interfacial tension of 0.12–0.25 mN/m at 80–120 °C and rapidly reversed rock wettability from strongly oil-wet to water-wet, reducing the contact angle from 141.7° to 38.9° within 80 min. The positively charged surface inhibited clay swelling, achieving 92% at 0.20 wt%. Core flooding and NMR T2spectra revealed that alternating CO2and FN-GQDs injection at a 2:1 gas–water ratio achieved a final oil recovery of 52.5%, significantly higher than pure CO2flooding. Through synergistic effects of interfacial tension reduction, wettability alteration, viscosity enhancement, and anti-swelling, FN-GQDs improve microscopic displacement efficiency and macroscopic sweep volume, showing great potential for CO2-enhanced oil recovery in shale reservoirs.
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Nano Energy
·
2026
·
IF 16.7
Hongguang Sun,Biaolin Peng,Kun Peng,Yi Chen,Ying Qiu,Min Wang,Kanghua Li,Zhonghui Shen,Tianwen Dong,Liang Liao,Ming Luo,Shujun Zhang,Xi Xiao,Qiuyun Fu,ZhongLin Wang
A novel ferroelastic domain reconfiguration mechanism delivers giant thermo-optic response in sol–gel barium titanate (BTO) thin films. • 750 °C-annealed BTO achieves record thermo-optic coefficient of 9.69×10⁻⁴ °C⁻¹, multiple times higher than silicon. • Fully reversible a-c domain switching is realized within the practical 25 °C-100 °C working window for on-chip photonic devices. • Prototype Fabry-Pérot modulator reaches 40% modulation depth for photonic integrated circuits and wavelength selective switching. • The domain engineering strategy is potentially extendable to CMOS-compatible ferroelectric perovskites. Thermo-optic tuning in ferroelectric perovskites has emerged as a promising strategy for achieving broadband, energy-efficient optical modulation. Nevertheless, the physical origins governing the near-room-temperature thermo-optic response of ferroelectrics are still not clearly defined. In this work, we report a previously unexplored thermo-optic mechanism in sol-gel-processed (001)-oriented BaTiO₃ films. This mechanism is driven by thermally induced, reversible a-c ferroelastic domain switching, activated through the synergistic control of interfacial screening, domain, and defect engineering, resulting in a large change in refractive index in the 25°C-100 °C temperature range. Films crystallized at 750 °C exhibit high tetragonal phase purity, strong c-axis alignment, and an exceptional thermo-optic coefficient of 9.69×10 -4 °C⁻¹—over several times larger than that of crystalline silicon—together with reversible domain evolution upon thermal cycling. A Fabry-Pérot thermo-optic modulator incorporating this film delivers 40% intensity modulation in the C-band. These findings establish a ferroelastic-domain-driven route to thermo-optic enhancement in ferroelectric perovskites and lay the groundwork for scalable. Download: Download high-res image (175KB) Download: Download full-size image Fig. X: TOC Graphic.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Wenxing Xin,Qian Wang,Dong Yang,Ao Yan,Yiwei Yu,Enfeng Zhang,Shaojie Qin,Xiping Zhang,Ziyi Zhu,Yiyong Zhang,Xue Li
Lithium metal batteries suffer from unstable interphases due to uncontrolled dendrite growth and continuous electrolyte depletion. Research indicates that constructing a solid electrolyte interphase (Hereinafter referred to as SEI) film enriched with lithium fluoride (LiF) can effectively suppress lithium dendrite growth and reduce the occurrence of side reactions. However, existing research has primarily focused on the formation mechanism of LiF and its concentration within the SEI, while neglecting a critical parameter in the film formation kinetics—the LiF formation rate. This limitation has resulted in a lack of effective strategies for rapidly forming high-performance SEIs rich in LiF. This work introduces a cobalt-based organometallic salt additive, wherein the exchange reaction between cobalt and lithium ions in situ generates a catalytic metallic cobalt layer. Through active sites on the cobalt layer surface, TFSI − anions are strongly adsorbed and catalyzed to decompose at the interphase. This layer synergistically optimizes the electrolyte to promote LiF formation. This strategy effectively accelerates TFSI − decomposition, hastens Li + desolvation, reduces nucleation overpotential, and guides uniform lithium deposition/stripping, thereby significantly suppressing dendrite propagation. Consequently, the Li||Cu half-cell achieves a Coulombic efficiency of up to 99.77% under the Aurbach test; while the Li||LFP full cell retained 72.37% capacity after 400 cycles under stringent conditions (N/ P = 1.1, 1 C). This site-specific catalytic strategy provides an effective pathway for constructing stable inorganic-rich SEI layers, paving a promising road toward developing safe and durable lithium metal batteries.
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Catalysts
·
2026
·Vol. 16
·期 2
·129
·
IF 4
Jing Yan,Junbing Tao,Fengxu Xiao,Guiyang Shi,Youran Li
This study systematically characterized the L-cysteine biosynthetic pathway inBacillus licheniformisand demonstrated that exogenous serine supplementation significantly upregulated the expression of pathway-associated genes, confirming serine as the primary precursor driving L-cysteine synthesis. Through targeted gene deletions, we generated knockout strains BL2ΔglyA, BL2ΔsdaAA, BL2ΔmetC, BL2Δ2, and BL2Δ3 to minimize precursor diversion and product degradation. Combinatorial overexpression of the feedback-resistant mutantcysEfand the transportereamAyielded an engineered strain achieving 1.075 g/L L-cysteine in shake-flask fermentation with an 18.69% molar conversion yield. These findings highlight the potential ofB. licheniformisas a platform for sulfur metabolic engineering and provide a sustainable fermentation strategy to replace traditional high-pollution hydrolysis-based L-cysteine production. Additionally, this work reveals fundamental differences in sulfur metabolism networks between Gram-positive and Gram-negative bacteria, elucidating microbial metabolic diversity and the cross-regulatory mechanisms linking sulfur, carbon, and nitrogen metabolism.
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