BIOMATERIALS
·
2015
·
IF 8.557
Jiao Wang,Haoyue Wang,Rongrong Zhu,Qiang Liu,Jian Fei,Shilong Wang
Sepsis is a significant public healthcare problem, affecting millions of people worldwide each year, killing one in four, and increasing in incidence. Thus, advanced therapeutic strategies are required to treat sepsis patients. Curcumin (Cur) is a promising anti-inflammatory agent for various inflammatory disorders. However, the therapeutic efficacy of Cur is limited due to poor aqueous solubility, rapid degradation, and low bioavailability. The aims of this study were to evaluate the therapeutic potential of Cur-loaded solid lipid nanoparticles (Cur-SLNs) for sepsis treatment. A firefly luciferase transgenic mouse was used to monitor real time interleukin 1β (IL-1β) expression in lipopolysaccharide (LPS)-induced sepsis model to examine the protective effect of Cur-SLNs, and to elucidate its underlying molecular mechanisms. Mice (female or male) were intraperitoneally administered with free Cur or Cur-SLNs (30 mg/kg) before the intraperitoneal delivery of LPS (3 mg/kg). Our results indicated that Cur-SLNs can effectively reduced levels of IL-1β expression compared to free Cur, especially at 3 h after LPS injection. Also, Cur-SLNs significantly decreased the expression of serum pro-inflammatory cytokines, including IL-6, TNF-α, and IL-1β as compared with free Cur, but augmented anti-inflammatory cytokine IL-10 by ELISA assay. Further, marked alleviation of the sepsis-induced damage to organs, including kidney, liver, and heart was observed with Cur-SLNs treatment as determined by hematoxylin/eosin-staining. Western blot analyses revealed that Cur-SLNs can significantly lower the expression levels of TLR4 , TLR2 , and TNF-α in lymph node tissues. Meanwhile, it showed suppressions of NF-κB activation and IκBα degradation levels. In conclusion, we suggested that Cur-SLNs may be used as an effective and safe therapeutic agent in treating sepsis in high-risk patient groups.
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FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yaqing Xiao,Yingnan Liu,Shufang Kang,Mengdi Cui,Huaide Xu
Spherical curcumin/polyvinylpyrrolidone nanocapsules (CurNC) with the improved water solubility and thermal stability than native curcumin (Cur) were synthesized. Subsequently, soy protein isolate-based pH-sensitive multifunctional packaging films reinforced with cellulose nanocrystals (CNC), CurNC, CNC/CurNC, and CNC/Cur were developed for shrimp freshness detection. It was found that adding the CNC-containing agents improved the tensile strength, hydrogen bond interactions, thermal stability, and crystalline structure of the film and decreased its water solubility and water vapor and oxygen permeability. The Cur-containing agents decreased the L* (lightness/darkness) and a* (redness/greenness) values and increased the b* (yellowness/blueness) and ΔE (total color difference) values of the film. A more dense and uniform matrix of the nanocomposite films was likely responsible for delaying the release of Cur from the film matrix. Interestingly, CNC/CurNC film displayed higher antiradical scavenging activity than CNC/Cur film, as well as remarkable color responsiveness to pH changes and NH 3 presence. Moreover, the CNC/CurNC film was able to decrease the total volatile basic nitrogen of stored shrimp and visually monitor shrimp freshness in real-time.
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Food Packaging and Shelf Life
·
2025
·
IF 10.6
Xutao Mai,Yuheng Yang,Daoying Wang,Weimin Xu,Zhilan Sun,Fang Liu
To enhance the solubility and stability of curcumin, a novel double-crosslinked antimicrobial hydrogels OHA/GEL/CUR-Zn(II) was prepared. Curcumin, as a ligand, was complexed with Zn 2+ to form a stable complex (CUR-Zn(II)), which was grafted to the hydrogel formed by oxidized hyaluronic acid (OHA) and gelatin (GEL) through Schiff base reaction. Fourier transform infrared spectroscopy analysis was employed to determine the formation of Schiff base (C N) between OHA with GEL, as well as the ester bond between OHA with CUR-Zn(II). X-ray diffraction results showed the presence of amorphous peaks in the composite film. The change in crystal structure was probably due to the formation of new hydrogen bonding or chemical bonding between CUR-Zn(II) and OHA/GEL. The incorporation of CUR-Zn(II) complexes into a hydrogel matrix resulted in a significant enhancement of tensile strength (TS) and elongation at break, accompanied by a reduction in water vapor transmission rate. Meanwhile, the hydrogel achieved a bactericidal rate of over 99 % against the predominant spoilage bacteria Escherichia coli and Staphylococcus aureus in chilled chicken meat, extending the shelf life of the chilled chicken meat to over 8 days. Overall, the composite hydrogel enhanced the stability of curcumin by chelating it with Zn 2+ and increased its solubility by grafting it onto OHA and GEL cross-linked hydrogels, demonstrating significant application potential in food packaging.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Boshan Shi,Gang Chen,Maolin Tu,Chao Zhao,Chao Liu,Lingyi Liu,Mohamed A. Farag,Lianliang Liu
Proteins and polysaccharides are widely used to construct drug delivery systems, while oat polyphenols exhibit strong antioxidant properties. Despite its antioxidant capacity, curcumin remains inherently unstable. In this study, sodium caseinate (SC) -polyphenol and SC-polysaccary-polyphenol conjugates were prepared by ultrasound-assisted enzymatic method, and the HIPE system was constructed to improve the stability of curcumin. Intrinsic fluorescence spectroscopy demonstrated a marked decrease in maximum emission intensity of SC-chitosan oligosaccharide (COS) conjugates, confirming fluorescence quenching through covalent SC-polysaccharide cross-linking. SC-CMC conjugates exhibited significantly higher surface hydrophobicity. In vitro digestion experiments demonstrated that HIPEs prepared with CMC achieved higher curcumin retention rates. Curcumin retention in HIPE systems showed no significant variation across pH 3.0–7.0, with polyphenol incorporation effectively mitigating pH-dependent retention fluctuations ( p < 0.01). Notably, the pH sensitivity of curcumin was reduced by the addition of polyphenols. The HIPE-based system significantly enhanced curcumin stability and shows potential for the targeted delivery of bioactive conjugates.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zenghong Xing,Hongtao Chen,Yuzhong Yan,Deming Gong,Guowen Zhang
In this study, the egg white protein (EWP)-tannic acid (TA)-xanthan gum (XG) ternary conjugate was used to construct emulsion and oleogel delivery systems to load curcumin. The effect of EWP-TA-XG conjugate concentration on the properties of emulsions and oleogels as well as the digestion characteristics of curcumin-loaded emulsion and oleogel were investigated. It was found that with the increase of EWP-TA-XG conjugate concentration, the D[4, 3] of emulsions decreased, while the absolute value of zeta potential and the stability of the emulsions increased. All emulsions showed good stability after centrifugation at different temperature, pH and ionic strength. Additionally, the emulsions at high concentrations of EWP-TA-XG conjugate (0.6–1.0 %, wt) had high viscosity and good gel property. As the concentration of EWP-TA-XG conjugate increased, the oil binding capacity of oleogels enhanced (90.38–99.87 %), and all oleogels showed solid behaviors (G′ > G″). The oleogels had a higher antioxidant activity than bulk oil, and their antioxidant capacity was positively correlated with the concentration of EWP-TA-XG conjugate. It was also found that the free fatty acid release rate and bioaccessibility of curcumin in the emulsion (66.63 % and 65.53 %) and oleogel (64.24 % and 62.20 %) systems were significantly improved compared to those in bulk oil (30.63 % and 29.75 %), indicating that the emulsion and oleogel delivery systems played a good protective role in the delivery of curcumin. This study may offer novel insights into the development of EWP-TA-XG conjugates-based emulsion and oleogel as delivery systems in encapsulating and delivering fat-soluble active substances.
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Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 24
·3884-3893
·
IF 5.047
Yuancheng Liu,Fan Chen,Kui Zhang,Quan Wang,Yuanwei Chen,Xianglin Luo
Conventional cross-linked micelles may suffer from low drug loading efficiency, side effects or toxicity of residual cross-linkers and complicated preparation processes of drug-loaded micelles. Herein, pH-sensitive reversibly cross-linked micelles by phenol–yne click via curcumin (Cur) were developed by combining drug loading and cross-linking using poly(ethylene glycol)-b-poly(2-methacrylate ethyl 5-hexynoicate), (mPEG-b-PHEMA-5HA), as a drug delivery system. mPEG-b-PHEMA-5HA was synthesized by ATRP of 2-hydroxyethyl methacrylate (HEMA) using mPEG-Br as a macroinitiator and then by esterification with 5-hexynoic acid. mPEG-b-PHEMA-5HA could form micelles and the micelles could load Cur. Meanwhile, the Cur loaded micelles were readily cross-linked by a phenol–yne click reaction. The cross-linked micelles showed excellent stability after high dilution with water, and turned into smaller micelles under an acid environment (pH 5.0). The cross-linked micelles achieved a higher drug loading of Cur (17.81%, wt%) than the noncross-linked micelles, due to physical entrapment and chemical click reaction. Cur release from the Cur-cross-linked micelles was inhibited under physiological conditions (pH 7.4) but was faster at pH 5.0 in comparison with the noncross-linked micelles. The cross-linked micelles exhibited a superior antitumor activity with a lower IC50 of 4.86 and 9.6 μg mL−1 toward HeLa and 4T1 cells, respectively, resulting from effective internalization by tumor cells. In pharmacokinetics, the cross-linked micelles showed a prolonged circulation time, and thus exhibited more effective inhibition of tumor growth compared to free Cur and Cur loaded noncross-linked micelles in in vivo experiments. Therefore, pH-sensitive reversibly cross-linked micelles via Cur are promising as an antitumor drug delivery system.
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International Journal of Nanomedicine
·
2023
·
IF 8
Lian Deng,Xiongjie Zhu,Zhongjian Yu,Ying Li,Lingyu Qin,Zhile Liu,Longbao Feng,Rui Guo,Yanfang Zheng
Background Although single-drug chemotherapy is still an effective treatment for esophageal cancer, its long-term application is limited by severe side-effects, poor bioavailability, and drug-resistance. Increasing attention has been paid to nanomedicines because of their good biological safety, targeting capabilities, and high-efficiency loading of multiple drugs. Herein, we have developed a novel T7 peptide-modified pH-responsive targeting nanosystem co-loaded with docetaxel and curcumin for the treatment of esophageal cancer.Methods Firstly, CM-β-CD-PEI-PEG-T7/DTX/CUR (T7-NP-DC) was synthesized by the double emulsion (W/O/W) method. The targeting capacity of the nanocarrier was then investigated by in vitro and in vivo assays using targeted (T7-NP) and non-targeted nanoparticles (NP). Furthermore, the anti-tumor efficacy of T7-NP-DC was studied using esophageal cancer cells (KYSE150 and KYSE510) and a KYSE150 xenograft tumor model.Results T7-NP-DC was synthesized successfully and its diameter was determined to be about 100 nm by transmission electron microscopy and dynamic light scattering. T7-NP-DC with docetaxel and curcumin loading of 10% and 6.1%, respectively, had good colloidal stability and exhibited pH-responsive drug release. Good biosafety was observed, even when the concentration was as high as 800 μg/mL. Significant enhancement of T7-NP uptake was observed 6 hours after intravenous injection compared with NP. In addition, the therapeutic efficacy of T7-NP-DC was better than NP-DC and docetaxel in terms of growth suppression in the KYSE150 esophageal cancer model.Conclusion The findings demonstrated that T7-NP-DC is a promising, non-toxic, and controllable nanoparticle that is capable of simultaneous delivery of the chemotherapy drug, docetaxel, and the Chinese Medicine, curcumin, for treatment of esophageal cancer. This novel T7-modified targeting nanosystem releases loaded drugs when exposed to the acidic microenvironment of the tumor and exerts a synergistic anti-tumor effect. The data indicate that the nanomaterials can safely exert synergistic anti-tumor effects and provide an excellent therapeutic platform for combination therapy of esophageal cancer.
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Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 1
·139-149
·
IF 5.068
Qi Mengjiao,Zou Shaohua,Guo Chunjing,Wang Kaili,Yu Yueming,Zhao Feng,Fan Huaying,Wu Zimei,Liu Wanhui,Chen Daquan
In this paper, a novel pH and redox dual-sensitive nanocarrier loaded with curcumin (Cur) and anticancer polypeptide (AP) was developed for dual targeting mitochondrial and CD44 receptor. The amphiphilic block copolymer was prepared by triphenylphosphonium (TPP)/oligomeric hyaluronic
acid (oHA)/disulfide-menthone 1,2-glycerol ketal (SM), hereinafter referred to as TPP-oHSM. The TPP targeted the mitochondria, pH/redox dual-sensitive SM served as a hydrophobic part, and the CD44 receptor targeting oHA worked as a hydrophilic part. The chemical structure of the TPP-oHSM was
identified using 1H NMR and FTIR technologies. Cur and AP were loaded into the TPP-oHSM micelles by self-assembly and denoted as C/A@TM. The C/A@TM prepared in this study exhibited an approximately spherical structure, with a mean diameter of 191.3 ± 3.1 nm and a negative
zeta potential of –26.10 ± 0.45 mV. The in vitro release study and cellular uptake test revealed that the C/A@TM targeted the mitochondria and CD44 receptor, as well as it showed sensitivity towards pH and redox. In addition, the C/A@TM demonstrated satisfactory cytotoxic
effects against MDA-MB-231 cells and MCF-7 cells. Finally, the in vivo application of the C/A@TM showed excellent therapeutic effects. The C/A@TM developed in this study exhibited promising multifunctional properties as a co-delivery carrier of polypeptide and chemical drug for an effective
clinical therapy for cancer.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2017
·
IF 2.329
Yingnan Liu,Shaoxuan Yu,Lizhi Liu,Xiaoyue Yue,Wentao Zhang,Qingfeng Yang,Li Wang,Yanru Wang,Daohong Zhang,Jianlong Wang
In this work, the double-edged effect of curcumin (Cur) on Maillard reaction (MR) in model milk pasteurization process was demonstrated for the first time, where Cur inhibits MR at low concentrations (<5 μg/mL), while promotes it at high concentrations (>5 μg/mL). A nanocapsule strategy was introduced to resolve the disadvantages of Cur on MR at high temperature. In vitro experiments confirmed that curcumin @ polyvinylpyrrolidone nanocapsules (Cur@PVP NCs) synthesized by a one-pot method can control the continuous and slow release of Cur and exert inhibitory effect on MR by competing with lactose for casein, trapping the reactive dicarbonyl compounds and scavenging ROS, which might provide a new strategy for the application of functional food ingredients in the control of MR during food heat processing , including milk pasteurization.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Zijun Wang,Huan Li,Shikai Zhang,Ke Ding,Ying Xie,Rongrong Wang,Liang Zhao,Wenjiang He,Yang Shan,Shenghua Ding
Plant proteins are increasingly used to encapsulate hydrophobic nutrients to formulate functional foods. A major challenge for plant protein-based nanocarriers is to improve the solubility and stability, thereby enhancing the absorption efficiency and bioavailability of hydrophobic nutrients within them. To address this issue, a free radical grafting strategy of folic acid (FA) modified pea protein isolate (PPI) was developed. The FA loading capacity of PPI-FA conjugates was 2.1-fold higher than that of PPI-FA mixtures. Conjugation of FA improved the solubility, thermal stability and storage stability of PPI. The photostability of FA was also enhanced (retention rate increased from 75.76 % to 94.92 %). Complexation with PPI-FA conjugate elevated the solubility, anti-cancer activity and cellular uptake of curcumin (Cur). The bioavailability of PPI-FA-Cur was 63.27 %, far exceeding those of free Cur (6.95 %) and PPI-Cur (24.47 %). These findings expand PPI's application and provide new reference for the development of advanced food delivery systems.
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MATERIALS & DESIGN
·
2025
·
IF 7.9
Peng Luo,Jiaming Fu,Xiaowei Yang,Fengbo Mo,Yanlong Zhong,Qi Lai,Shaorong Huang,Xiaoyong Zhang,Bin Zhang,Yen Wei
Osteoporosis is one of the most common chronic metabolic diseases that mainly caused by an imbalance in bone homeostasis. Considered its severe concurrent disorders, osteoporosis has become a global health issue, however, the anti-osteoporosis medications available in clinical practice are confronted with challenges such as high costs, adverse side effects, and limited efficacy. Herein, we reported construction of pH-responsive and bone-targeting nanomedicine (termed HPGCA) via click conjugation of alkyne functionalized hyperbranched polyglycerol (HPG) with curcumin (Cur) and alendronate (ALN) for the first time. We demonstrated that HPGCA exhibit superior water dispersibility, small particle size, and pH-dependent drug release behavior. Results from biological assays indicated that HPGCA displayed desirable bone-targeting capability, strong anti-osteoporosis efficacy, good hemocompatibility, and negligible adverse effects. More importantly, HPGCA showed better anti-osteoporosis efficacy than both free Cur and ALN according results from micro-CT and TRAP staining. The synergistic osteoporosis therapeutic efficacy would be explained by targeting delivering HPGCA to bone tissues and in-situ releasing cargos under acidic environment. Therefore, considered the above aspects, we believe that the innovative nanomedicine is promising for osteoporosis therapy in clinic and will provide new insights for full realization anti-osteoporotic properties of natural compounds
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Gang Liu,Qi Wang,Zhongze Hu,Jie Cai,Xinguang Qin
In this study, whey protein isolates (WPI), lactose (Lac) Maillard-reacted products, and epigallocatechin gallate (EGCG) complex were used to enhance the thermal stability of the Pickering emulsion delivery of curcumin. Atomic force microscopy combined with Fourier transform infrared spectroscopy were employed to study the morphological characteristics and structural changes of WPI-Lac/EGCG nanoparticles. Results proved that WPI-Lac and EGCG were combined by hydrogen bonding and hydrophobic interaction. The mechanism underlying WPI-Lac/EGCG-stabilized Pickering emulsion was further characterized by confocal laser scanning microscopy. The optimal binding ratio of WPI-Lac to EGCG was 1:1 (w/w) at pH 3.0. The particle size and zeta potential of the WPI-Lac/EGCG nanoparticles were about 110 nm and 27 mV, respectively. Analysis of microstructure and droplet size distribution revealed that the glycated WPI-Lac/EGCG-stabilized emulsions exhibited more uniform droplet distribution, stronger thermal stability, and higher curcumin percentage retention than WPI. These results indicate that the WPI-Lac/EGCG nanoparticles are potential stabilizers for Pickering emulsion requirements. This study provides a basis for the construction of Pickering emulsion systems while carrying pro-/hydrophobic bioactive components.
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