APPLIED SURFACE SCIENCE
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2026
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IF 6.6
Fang Miao,Mingquan Jiang,Jing Yu,Yiyan Wang,Jiehong Lei
Dual-layer Ag 0 -HA@ACF enables rapid iodine capture and durable immobilization. • HA-driven in situ reduction achieved 21.58 wt% uniformly dispersed Ag 0 loading. • Ag 0 -HA@ACF achieves 1934.7 mg/g iodine uptake, surpassing most Ag-based adsorbents. • DFT revealed strong chemisorption of I 2 on Ag 0 (−3.880 eV) with AgI formation. A dual-layer active interface synergistic strategy was innovatively developed to address the critical challenge of efficient capture and immobilization of radioactive iodine vapor during spent fuel reprocessing. Humic acid (HA)-grafted activated collagen fiber (ACF) was constructed as a rapid induced adsorption layer, while nanoscale zero-valent silver (21.58 wt%) was in situ immobilized through HA-assisted chelation/double reduction combined with NaBH 4 reduction, forming a specific chemical immobilization layer. The resulting Ag 0 -HA@ACF composite exhibited an outstanding iodine-vapor uptake of 1934.7 mg/g and achieved 81.2% of its saturated capacity within 6 h, outperforming most reported silver-based adsorbents. FT-IR, XPS, and DFT analyses systematically revealed the synergistic “induced adsorption–specific immobilization” mechanism, where phenolic hydroxyl and amino groups promoted rapid I 3 ⁻ formation, while nanoscale Ag 0 converted iodine into thermally stable AgI. Moreover, DFT calculations revealed a strong chemisorption interaction between Ag 0 and I 2 , with an adsorption energy of − 3.880 eV. Owing to its facile preparation and scalability, this material provides a promising strategy for radioactive iodine management. Download: Download high-res image (124KB) Download: Download full-size image
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xiaozhou Li,Bin Wu,Zhen Gao,Wei Zhuang
To address the challenges of quantitative immobilization and unavoidable spatial hindrance of multiple enzymes in traditional multi-enzyme cascade immobilization, we developed a novel bienzyme immobilization system based on a hierarchically structured MXene@LDHs@Fe₃O₄ (MLF) carrier. The MLF composite, with a high theoretical surface area (∼300 m 2 /g), adjustable interlayer spacing (10–50 nm), and strong magnetization (20 emu/g), provides an ideal support for high-capacity enzyme loading. The system was fabricated through the assembly of MXene nanosheets with layered double hydroxides (LDHs) and magnetic Fe₃O₄ nanoparticles, integrating horseradish peroxidase (HRP) on the MLF core, a polydopamine (PDA) interfacial barrier, and surface-immobilized glucose oxidase (GOx) through melamine-genipin mediated dendritic cross-linking. This spatially organized catalytic architecture enables quantitative enzyme loading while minimizing spatial hindrance, thereby enhancing catalytic efficiency. The co-immobilized bienzyme reactor (GOX@PDA@HRP@MLF) achieved loading capacities of 272.46 mg/g for HRP and 414.33 mg/g for GOx, with an activity recovery of 85.3 % during 2,4-dichlorophenol degradation. The immobilized enzymes retained ∼80 % of their initial activity after five operational cycles. Under optimized conditions, the GOX@PDA@HRP@MLF material achieved a degradation rate of 4.6 for 2,4-dichlorophenol in a continuous flow system, representing a ∼21-fold increase in space-time yield compared to random multi-enzyme immobilization on single-layer resin materials. These results demonstrate that the dendritic cross-linking strategy employed in this study can serve as a universal method for multi-enzyme immobilization, with significant potential for industrial applications.
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高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Meiqing Chen,Wanxia Li,Bo Ruan,Yuxin Gu,Qingcheng Zhang,Hongling Bu,Peixin Du,Peng Yuan
The interaction between birnessite and dissolved organic matter(DOM) is critical for carbon sequestration and heavy metal immobilization. This study investigated humic acid (HA, a representative form of DOM) in mediating the formation of triclinite birnessite (TB) and elucidated the influence mechanisms for carbon sequestration and As(V) immobilization. HA significantly affected both the growth and structural properties of TB. HA induced TB structural transformation from lamellar-like to honeycomb-like, reduced surface negative charge, and increased specific surface area, oxygen vacancies and hydroxyl groups. HA-Mn(III) complexes and pore trapping enhance organic carbon sequestration synergistically. As(V) immobilization was improved by inner-sphere complexation (via hydroxyl coordination) and electrostatic attraction with the maximum adsorption capacities for HA-mediated birnessite (TB-2H) were 65.140 mg·g⁻¹ , 6.4-fold higher than pure TB. Besides, TB-2H shows low manganese release, high stability and good reusability. These findings achieves synergistic carbon sequestration and As(V) immobilization, providing an innovative strategy for environmental remediation.
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高影响因子
POLYMER COMPOSITES
·
2026
·
IF 4.7
Mengfei Du,Enhao Zhu,Xilai Yan,Zhe Wang,Ke Wang
Enhancing the durability of ultraviolet-cured-in-place pipe (UV-CIPP) in alkaline environments remains challenging due to its long-term exposure to concrete pore solution from the original pipe. Herein, a novel strategy that γ-AlOOH coating assists to immobilize Irganox 1010 onto glass fibric (GF) was proposed for simultaneously improving alkaline corrosion resistance and mechanical properties of UV-CIPP. Through the beneficial bridging of γ-AlOOH with the coupling agent, Irganox 1010 was immobilized on the GF (GF-Al-g-1010) surface through hydrogen bonding and covalent bonding. The leaching of Si 2+ from the GF backbone of unsaturated polyester resin (UP)/GF-Al-g-1010 composite was reduced by 40.60% and the hydrolyzed alcohol products decreased by 47.1%. Meanwhile, the mechanical properties of UP/GF-Al-g-1010 remained well preserved. The flexural and tensile modulus retained at 99.0% and 96.3%, respectively. The immobilization of Irganox 1010 at the UP/GF interface formed a protective hydrophobic layer and stabilized free radicals, which together inhibited resin degradation and reduced interface debonding. Moreover, the flexural strength and modulus of composites increased by 36.5% and 38.4%, respectively. The chemical bonding between GF-Al-g-1010 and UP chains enhanced interfacial adhesion. In summary, this work will facilitate the design of UV/thermal dual-cured composites with high strength and alkaline corrosion resistance for pipeline rehabilitation. Highlights A novel AlOOH coating assists to immobilize Irganox 1010 onto glass fabric. The flexural strength and modulus increased 36.5% and 38.4%, respectively. The Si 2+ leaching and hydrolyzed alcohol products were reduced by 40.60% and 47.1%. The flexural and tensile modulus retention reached 99.0% and 96.3%, respectively. The AlOOH-1010 coating improved both durability and mechanical properties.
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高影响因子
Advanced Sensor and Energy Materials
·
2024
·
IF 0
Hongyan Yang,Zeyu Ma,Dan Zhang,Yi Wang,Lei Li,Guobao Zhou
Although immobilization-free and label-free electrochemical DNA (E-DNA) biosensors have engaged tremendous interest due to their superior properties, such as easy operation, time-saving and cost-saving, most of them are fabricated in homogeneous modes and usually produce high background current. In the present work, we proposed a new immobilization-free and label-free heterogeneous E-DNA assay based on a dual-blocker-aided multibranched hybridization chain reaction (HCR) for one-pot nucleic acid detection with zero background. The target nucleic acid triggers the HCR involving cascaded hybridization between two metastable hairpins, resulting in the generation of HCR products with multibranched arms, which can be captured onto the electrode via π-π stacking interactions between multibranched arms and reduced graphene oxide (rGO). Prior to the incubation process with an electrode, two blockers are designed to prohibit the nonspecific absorption of unreacted hairpin probes. Thus, an immobilization-free and label-free heterogeneous electrochemical assay for one-pot nucleic acid detection with zero background is readily realized. This strategy also presents additional merits of simplicity and cheap cost, since probe immobilization, signal tag labeling, and multiple incubation processes are avoided. Therefore, the as-proposed effective and versatile biosensor has great potential to be applied in nucleic acid-related practical biosensing.
研究领域:
生物传感技术
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分子生物学
·
分析化学
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DOI
BIORESOURCE TECHNOLOGY
·
2019
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IF 6.669
Li-Qun Jin,Bo Yang,Wei Xu,Xian-Xiao Chen,Dong-Xu Jia,Zhi-Qiang Liu,Yu-Guo Zheng
In this study, recombinant E. coli BL21(DE3)/pCDFDuet-1-XR-GDH harboring xylose reductase (XR) and glucose dehydrogenase (GDH) were immobilized and applied for the production of xylitol from xylose mother liquor (XML). Various immobilization methods were screened and the cross-linking approach with diatomite and polyetherimide as the raw materials and glutaraldehyde as the cross-linking agent was the optimal one, and the recovery activity reached of 80.3% after immobilization. The half-life of immobilized cells was 1.52 times to that of free cells. Batch experiments showed that the enzyme activity of immobilized cells remained 70.5% of the initial activity after 10 batches and the space-time yield of xylitol reached of 11.5 g/(L h). The production of xylitol from xylose mother liquor by immobilized E. coli cells containing xylose reductase and glucose dehydrogenase was reported for the first time, which paved a foundation for industrial production of xylitol from waste xylose mother liquor.
研究领域:
生物工程
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代谢工程
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工业微生物学
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高影响因子
CHEMISTRY OF MATERIALS
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2014
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IF 8.535
Weifei Li,Xuan Liu,Guangyue Qian,Jianping Deng
The present study originally created a methodology for immobilizing polymeric nanoparticles on graphene oxide (GO) by chemical bonds, aiming at chiral functionalization of GO and simultaneously improving the dispersibility of polymer nanoparticles. To prepare the novel GO hybrids, GO was first prepared and alkynylated to form an actual comonomer (MGO), in the presence of which acetylenic monomer (M1) underwent emulsion polymerization in aqueous media with sodium dodecyl sulfate as emulsifier and (nbd)Rh+B–(C6H5)4 as catalyst. SEM and HRTEM images ascertained the formation of polymeric nanoparticles immobilized on GO (NPpoly1/GO). CD and UV–vis absorption spectra demonstrated the optical activity of NPpoly1/GO, originated from the chiral helical conformations adopted by the polymer chains constructing the nanoparticles. FT-IR, Raman, XPS, XRD, and TGA techniques were also utilized to characterize the GO hybrids. The as-obtained NPpoly1/GO was further used as a chiral additive to perform enantioselective crystallization of l-alanine from racemic alanine. l-Alanine was preferentially induced to crystallize rod-like crystals, according to SEM, CD, and XRD characterizations. The present study provides a versatile platform for preparing GO-derived functional materials, particularly novel chiral materials.
研究领域:
材料科学
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高分子化学
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纳米技术
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手性化学
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表面功能化
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高影响因子
INORGANIC CHEMISTRY
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2026
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IF 4.7
Shu Xian Wang,Kai Li,Yun Huai Zhang,Shuai Ma,Li Zhi Zhu,Yun Gong,Jian Hua Lin
Sodium–iodine batteries offer high voltage and theoretical capacity but suffer from iodine dissolution, shuttle effect, and sluggish kinetics. This work develops a porous Cu/Ni–Mo metal–organic framework (MOF) formulated as CuIINiII(4,4′-bpy) (MoVIO4)2 (4,4′-bpy = 4,4′-bipyridine) that addresses these challenges through a synergistic mechanism. The material’s channels can physically confine iodine via hydrogen bonds, while copper sites facilitate chemical immobilization via the reversible conversion CuNi(4,4′-bpy)(MoO4)2 + (x/2) I2 = Cu1-xNi(4,4′-bpy)(MoO4)2 + x CuI, with an energy barrier of 0.45 eV. The structural stability of the Cu-vacant MOF is associated with its rigid multinuclear {Cu2Ni2} clusters and abundant Mo–O–M (M= Ni, Cu) linkages. Impressively, some specific crystalline planes of the MOF undergo reversible order–disorder transitions during charge–discharge processes. These dynamic transformations are related to the adsorption of I2 on the Mo/Ni centers and the lattice O2– of these surfaces, promoting I–I bond elongation and cleavage, significantly enhancing iodine redox kinetics. Consequently, the as-fabricated Na–I2 battery shows a capacity of ∼250 mAh g–1 at 0.3 A g–1 with capacity retention (∼100 mAh g–1) over 1000 cycles at 2 A g–1.
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PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2017
·Vol. 93
·期 2
·489-497
·
IF 3.135
Xinyu Jin,Saisai Li,Nengbing Long,Ruifeng Zhang
BACKGROUND Immobilization of more than one peroxidase on the same support is an ideal technology for application because such a multi-enzyme catalytic system may show high activity over a very wide optimal range of pH, temperature and H 2 O 2 concentration. In this work, chloroperoxidase (CPO) and horseradish peroxidase (HRP) were co-immobilized on ZnO nanowires/macroporous SiO 2 composite support through an in situ cross-linking method. An anionic bi-epoxy cross-linker was used by adsorption on the surface of ZnO nanowires before cross-linking. RESULTS The cross-linking was carried out under suitable conditions: pH 6.5, reaction temperature 15°C and reaction time 15 h. Using a 1/1 mixture of CPO and HRP resulted in a co-immobilized enzyme with loading 79.6 mg CPO g -1 support and 52.8 mg HRP g -1 support , and total specific activity up to 15.7 U per mg support . The co-immobilized enzyme also showed good stability after 60 days of storage, and excellent reusability over 20 repeat uses. CONCLUSIONS For the decolorization of azo dyes the co-immobilized CPO (60%)/HRP (40%) exhibited high catalytic activity over very wide ranges of pH, temperature and H 2 O 2 concentration. Using this robust biocatalyst, complete decolorizations of azo dyes have been realized within 3 h of reaction. © 2017 Society of Chemical Industry
研究领域:
生物催化
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环境生物技术
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酶工程
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纳米材料
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废水处理
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Bo Ruan,Pingxiao Wu,Meiqing Chen,Xiaolin Lai,Liya Chen,Langfeng Yu,Beini Gong,Chunxi Kang,Zhi Dang,Zhenqing Shi,Zehua Liu
In this study, batch experiments were carried out to evaluate the biodegradation of phenol by Sphingomonas sp. GY2B, which were immobilized in polyvinyl alcohol (PVA) – sodium alginate – kaolin beads under different conditions. The optimal degradation performance was achieved by GY2B immobilized in beads containing 1.0% (w/v) of kaolin, 10% (w/v) of PVA, 0.3% (w/v) of sodium alginate, 10% (v/v) of biomass dosage, and exposed to an initial phenol concentration of 100 mg/L. The experimental results indicated that PVA-sodium alginate-kaolin beads can accelerate the degradation rate of phenol by reducing the degradation time and also improve degradation rate. The biodegradation rate of phenol by immobilized cells (16.79 ± 0.81 mg/(L·h)) was significantly higher than that of free cells (11.49 ± 1.29 mg/(L·h)) under the above optimal conditions. GY2B immobilized on beads was more competent than free GY2B in degradation under conditions with high phenol concentrations (up to 300 mg/L) and in strong acidic (pH = 1) and alkaline (pH = 12) environments. Higher phenol concentrations inhibit the biomass and reduce the biodegradation rate, while the lower biodegradation rate at low initial phenol concentrations is attributed to mass transfer limitations. The Haldane inhibitory model was in agreement with the experimental data well, revealing that phenol showed a considerable inhibitory effect on the biodegradation by Sphingomonas sp. GY2B, especially at concentrations higher than 90 mg/L. Intra-particle diffusion model analysis suggests that adsorption of phenol by immobilized beads was controlled by both rapid surface adsorption as well as pore diffusion mechanism. It's worth noting that the presence of 1 mg/L Cr(VI) enhanced the biodegradation of phenol by free cells, while Cr(VI) showed no obvious impact on the removal of phenol by immobilized cells. In addition, immobilized cells were reused 16 times and removed 99.5% phenol, and when stored at 4 °C for 90 days, more than 99% phenol was removed. These results showed that immobilized cells can significantly improve the microbial degradation performance, and protect microorganisms against unfavorable environment. It is implied that PVA -sodium alginate-kaolin beads have great potential to be applied in a practical and economical phenolic wastewater treatment system.
研究领域:
环境生物技术
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微生物降解
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废水处理
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生物修复
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应用微生物学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Yoann de Rancourt de Mimérand,Kun Li,Cong Zhou,Xiaoyun Jin,Xiaoxian Hu,Yufei Chen,Jia Guo
Inorganic photocatalysts became an essential and powerful tool for the remediation of polluted water. However, important limitations of photocatalysts in their colloidal form, especially nanosized, remain. For instance, their separation from water after use and recovery, which can be particularly demanding, time- and energy-wise. Considering such aspects, supported catalysts bear significant advantages. However, efforts still have to be made to develop processes that allow the permanent and efficient immobilization of inorganic photocatalysts in sustainable conditions, in order to maintain the advantages of supported catalysts over colloidal ones. Herein, we report the use of an aqueous-phase plasma-aided grafting (APPAG) process to produce functional and efficient hybrid photocatalysts. More specifically, based on cold plasma discharge (CPD), ZnO/Bi2MoO6 heterojunctions were permanently immobilized on polymer supports generated by 3D-printing, with fractal-inspired designs. Three different approaches of the APPAG process have been successfully used for the immobilization of the inorganic phase, that is core–shell-assisted direct grafting, indirect grafting and in situ complexation-assisted precipitation (ISCAP). Noticeably, the latter technique has never been reported before to our knowledge. These three immobilization routes rely on different strategies and yield to distinct morphological specificities, but all allow using mild synthesis conditions and producing stable, active, permanently immobilized coatings of photocatalysts. Regarding the preparation of the organic supports, two sorts of additive manufacturing (AM) technologies were employed, namely fused-deposition modeling (FDM) and liquid crystal diode (LCD)-based SLA (stereolithography). The use of fractal geometries combined with AM permits the production of supports with relatively high surface areas, in a single processing step. Overall, the three plasma-based immobilization methods revealed to be efficient and the performance of the different hybrid photocatalysts have later been assessed through the photodegradation of Rhodamine B dye under simulated sunlight irradiation and visible light only, with promising results.
研究领域:
环境科学
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材料科学
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化学工程
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等离子体技术
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增材制造
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DOI
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2017
·
IF 2.24
Fei Chang,Saisai Xue,Xiaqing Xie,Wei Fang,Zemin Fang,Yazhong Xiao
Complicated purification steps, together with the fact that β-glucosidase has to be tolerant to ethanol restricts the application of β-glucosidase in isoflavone aglycone hydrolyzing process. β-Glucosidase Bgl1A(A24S/F297Y) is a promising enzyme in hydrolyzing isoflavones. In this work, six different carbohydrate-binding modules (CBMs), which were from 3 families, were fused to the C-terminal of Bgl1A(A24S/F297Y), respectively, to simplify the enzyme preparation process. The fusion proteins were expressed in Escherichia coli and adsorbed onto cellulose . The Bgl-CBM 24 was found to have the highest immobilization efficiency at room temperature within 1 h adsorption. Notably, 1-g cellulose absorbs up to 254.9 ± 5.7 U of Bgl-CBM 24 . Interestingly, the immobilized Bgl-CBM 24 showed improved ethanol tolerance ability, with the IC 50 of 35% (v/v) ethanol. Bgl-CBM 24 effectively hydrolyze soybean isoflavone glycosides . The hydrolysis rate of daidzin and gemistin was 85.22 ± 3.24% and 82.14 ± 3.82% within 10 min, with the concentrations of daidzein and genistein increased by 6.36 ± 0.18 mM and 3.98 ± 0.22 mM, respectively. In the repetitive hydrolytic cycles, the concentrations of daidzein and genistein still increased by 3.07 ± 0.24 mM and 1.94 ± 0.34 mM in the fourth cycle with 20% (v/v) ethanol. These results suggest that the immobilized Bgl-CBM 24 has excellent potential in the preparation of isoflavone aglycones.
研究领域:
生物化学
·
酶工程
·
食品科学
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高影响因子
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