MOLECULES
·
2023
·Vol. 28
·期 23
·7740
·
IF 4.6
Chen Luo,Xiaoxiao Chen,Pu Li,Chaobiao Huang
Based on DNA bio-dots-induced aggregation of gold nanoparticles (AuNPs), a methionine (Met) photoelectrochemical (PEC) sensor with CS–GSH–CuNCs/TiO2NPs as the photoelectric conversion element and AuNPs as the specific recognition element was constructed. First, a TiO2NPs/ITO electrode and CS–GSH–CuNCs were prepared, and then the CS–GSH–CuNCs/TiO2NPs/ITO photosensitive electrode was obtained by self-assembly. Next, DNA bio-dots were modified to the upper surface of the electrode using a coupling reaction to assemble the DNA bio-dots/CS–GSH–CuNCs/TiO2NPs electrode. Amino-rich DNA bio-dots were used to induce the aggregation of AuNPs on the electrode surface via Au–N interactions and prepare the AuNPs/DNA bio-dots/CS–GSH–CuNCs/TiO2NPs electrode. Due to the fluorescence resonance energy transfer (FRET) between CS–GSH–CuNCs and AuNPs, the complexation chance of electron-hole (e−-h+) pair in CS–GSH–CuNCs increased, which, in turn, led to a decrease in photocurrent intensity. When Met was present, AuNPs aggregated on the electrode surface were shed and bound to Met since the Au–S interaction is stronger than the Au–N interaction, resulting in the recovery of the photocurrent signal. Under optimal conditions, the photocurrent intensity of the PEC sensor showed good linearity with the logarithm of Met concentration in the range of 25.0 nmol/L–10.0 μmol/L with the limit of detection (LOD) of 5.1 nmol/L (S/N = 3, n = 10).
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高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 45
·e202304084
·
IF 2.1
Xiaoyu Zhang,Zekun Li,Mengge Shi,Zhao Wang
Graphical The AuNPs size is precisely controlled in hydrogel composite films by glow discharge plasma. The low-temperature plasma not only prevents gold agglomeration but also safeguards the nanopores of the hydrogel from collapsing. The resulting hydrogel composite membrane catalyzes the reduction of MB by NaBH 4 , achieving complete degradation in just 12 seconds. Gold nanoparticles (AuNPs) were effectively synthesized and highly dispersed within a hydrogel composite by glow discharge plasma reduction. The hydrogel structure was successfully preserved due to the low temperature operation of plasma. The gold was reduced in situ within the hydrogel channels via electron reduction, and the uniformly distributed metal particles benefited from the confinement effect. The particle size could be controlled by adjusting precursor concentration and the type of hydrogel used. Hydrogels based on gelatin were found to increase the interaction between gold and the hydrogel. This enhancement was attributed to the Coulomb force arising from the isoelectric point and surface plasmon resonance (SPR) of the hydrogel composites, which promoted the dispersion of AuNPs. The different treatment procedures confirmed that SPR induced changes in the hydrogel surface, which played a key role in the dispersion and anchoring of gold during the reduction process. As a result, the AuNP-doped hydrogel composite exhibited significantly enhanced catalytic activity in the degradation of methylene blue, with AuNP-Gel-1 achieving complete MB degradation in just 12 seconds.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Jilin Yan,Xi Wen,Lixiu Yin,Yueju Wang,Huiling Li,Yifeng Tu
The aggregation of amyloid-β protein (Aβ) into amyloid-β oligomer (AβO) and finally the deposition of amyloid-β fiber (AβF) in brain was found strongly related to Alzheimer's disease (AD). In this work, an electrochemiluminescent (ECL) aptasensor reinforced with a gold nanoparticle – iron metal orgaic framwork nano-composite (AuNPs/Fe-MOFs) was constructed for the detection of Aβ. It has the ability to specifically quantify Aβ, the precursor that had not yet been aggregated into AβO. The employed AuNPs/Fe-MOFs nano-composite in this sensor not only provided the opportunity to anchor the aptamer, but also enhanced the response to promote its sensitivity. The relative inhibition ratio of luminol ECL on this sensor was linearly related to the logarithm of Aβ concentration in the range from 0.5 to 50 pM, with a LOD at 104 fM. The aptasensor has high specificity to Aβ over its analogs and recoveries in human serum were obtained within 96.0–103.7%. The research prospectively provides a new demonstration for early diagnosis of the disease in clinic.
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CELLULOSE
·
2019
·Vol. 26
·期 6
·4191-4204
·
IF 3.917
Cheng Deshan,Bai Xue,He Mantang,Wu Jihong,Yang Hongjun,Ran Jianhua,Cai Guangming,Wang Xin
Depositing anisotropic noble metal nanoparticles with high uniformity and yield on flexible substrates is the determining factor for surface enhanced Raman spectroscopy (SERS) detection. In this work, flexible, durable and sensitive SERS substrates were fabricated by in situ reduction of Ag nanoparticles on polydopamine templated cotton fabrics (CF) as catalytic hotspots to enhance the following deposition of Au nanoparticles. The coated CF were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy and X-ray diffraction to understand the surface morphology, chemical composition and crystalline structure, respectively. The SEM images indicate that the nanoparticles are dispersed evenly on the CF. 4-Mercaptobenzoic acid (4-MBA) has been used as the probe molecule to evaluate the sensitive and reproducible SERS properties of the as-fabricated SERS substrate. The as-prepared SERS substrates were demonstrated to detect carbaryl pesticides on a cucumber, and carbaryl with the concentration of as low as 10 −6 M (0.20 ppm) could be detected to ensure food safety.
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ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Wenjie Liu,Qiaofang Shi,Gang Zheng,Jun Zhou,Ming Chen
In this paper, gold nanoparticles (AuNPs) modified by thiolated calix[n]arene (TCnA, n = 4, 6, 8) were anchored on graphene nanosheets (GN) by π-π stacking interaction between TCnA molecules and GN. Testing of various electrochemical techniques display that the AuNPs@TCnA/GN modified glass carbon electrodes (GCE) show good electrocatalytic property , molecular recognition and enrichment performance toward dopamine (DA) and acetaminophen (AC), and exhibit high current response. In all of modified electrodes , AuNPs@TC8A/GN/GCE shows an outstanding electrochemical response for DA and AC with high sensitivity of 5.08 and 9.76 mA mmol −1 L cm −2 , respectively. The analytical curves for DA obtained by differential pulse voltammetry (DPV) are linear in the range from 0.5 to 150 μM and from 0.3 to 1.0 mM. The linear range for AC obtained by DPV is from 0.5 to 120 μM. Besides, AuNPs@TC8A/GN/GCE can be applied to determinate of DA and AC simultaneously, implying the prospect to develop a dual sensor under the same construction platform.
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Analytical Methods
·
2024
·
IF 2.7
Weiqiang Li,Xiaoxue Liu,Pianpian He,Wentao Hu,Kaijie Tang,Yangping Wen,Qian Zeng,Huiyi Tang,Yaxuan Lei,Xin Liu
Deoxynivalenol (DON) has drawn considerable attention for its obvious pathogenicity and wide use in agro-products, which cause a potential threat to human health. In this work, an electrochemical immunosensor is developed for the highly sensitive and selective detection of DON in wheat flour using AuNPs-BP-MWCNTs-COOH and antibodies. The AuNPs-BP-MWCNTs-COOH nanocomposite was prepared via an in situ reduction reaction and ultrasonic-assisted liquid-phase exfoliation. The nanocomposite exhibits a larger surface area, decent stability, excellent electron transfer capability, good protein binding capability and prominent specificity. The plentiful carboxyl group on the nanocomposite can bind to the amino group of the antibody, and AuNPs have an affinity for the sulfhydryl group of the antibody, which makes it feasible for the nanocomposite to load the antibody. The peak currents are plotted against the logarithm of DON concentration from 0.002 to 80 ng mL−1 with a limit of detection (LOD) of 0.5 pg mL−1. This approach establishes an effective label-free immunosensor platform for the detection of DON with high sensitivity and selectivity in various food and agricultural products.
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高影响因子
LUMINESCENCE
·
2017
·Vol. 33
·期 1
·119-124
·
IF 1.509
Nan Liu,Juan Hao,Keying Cai,Mulan Zeng,Zhenzhong Huang,Lili Chen,Bingxian Peng,Ping Li,Li Wang,Yonghai Song
A novel ratiometric fluorescence nanosensor for superoxide anion (O 2 •− ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb 3+ and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs. The AuNPs-BSA not only acted as a template to accelerate the growth of Tb/GMP NCPs, but also could be used as the reference fluorescence for the detection of O 2 •− . The resulting AuNPs-BSA@Tb/GMP NCP ratiometric fluorescence nanosensor for the detection of O 2 •− demonstrated high sensitivity and selectivity with a wide linear response range (14 nM–10 μM) and a low detection limit (4.7 nM).
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CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Pan Wang,Mingliang Du,Ming Zhang,Han Zhu,Shiyong Bao,Meiling Zou,Tingting Yang
Here we proposed a facile strategy to synthesize multi-layered gold nanoparticles/polyaniline/halloysite nanotubes (AuNPs/PANI/HNTs) nanostructures used for electrochemical sensors. The PANI/HNTs were firstly obtained by making use of the in situ polymerization as well as employing the thioglycollic acid (TA) as the dopant, thereafter, Au ions were anchored to TA and then reduced by PANI. Field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) observations implied that a large amount of uniform AuNPs were immobilized on the PANI/HNTs. The as-prepared AuNPs/PANI/HNTs nanostructures were also characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS), and the results confirmed the successful synthesis of AuNPs/PANI/HNTs nanostructures and explained the reactions in depth as well. Further investigations suggested that the AuNPs/PANI/HNTs nanostructures with well-separated AuNPs exhibited high electrochemical performance as sensors to detect hydrogen peroxide (H 2 O 2 ).
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NANO
·
2015
·
IF 1.09
YALI MAO,HUANBAO FA,YANMEI CHENG,YANQIU DU,WEI YIN,CHANGJUN HOU,DANQUN HUO,DONG ZHANG
A novel sensitive and elective electrochemical sensor was developed to detect methyl parathion (MP) based on a glassy carbon electrode (GCE) modified with gold nanoparticles ( AuNPs )/graphene nanocomposites film. The AuNPs were modified onto graphene sheets using NaBH 4 as a reductant. The obtained AuNPs /graphene nanocomposites were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The electrochemical behavior of MP and interference studies were then investigated. Compared with metal ions and nitroaromatic compounds, which exist in environmental samples, the AuNPs /graphene/GCE exhibited high adsorption and strong affinity toward MP. Under optimized conditions, the oxidation peak current of MP was linear to its concentration within the range of 4.0 × 10 -7 –8.0 × 10 -5 M, with a detection limit ( S/N = 3) of 8.5 × 10 -8 M. These results indicated that the AuNPs /graphene nanocomposites displayed a synergic effect involving the catalytic characteristics of graphene and AuNPs nanocomposites, which can effectively improve the electrochemical properties of MP. Furthermore, the AuNPs also enhanced sensor sensitivity to MP. Therefore, the AuNPs /graphene/GCE could be a promising sensor for the fast, sensitive and selective detection of MP in real samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yu Li,Ying Chen,Dongmei Deng,Liqiang Luo,Haibo He,Zhenxin Wang
In this work, a water-dispersible graphene/amphiphilic pyrene derivative nanocomposite has been synthesized for electrochemical immunosensing carcinoembryonic antigen (CEA). Water-soluble 4-armed poly(ethylene glycol)-NH 2 is immobilized on graphene through noncovalent functionalization with the assistance of pyrene as an anchor group. The noncovalent functionalization strategy can significantly improve the hydrophilicity of graphene and loading capacity of gold nanoparticles (AuNPs) on graphene, resulting in efficient immobilization of antibodies on the nanocomposite. The morphology and conductivity of the as-prepared nanocomposites were characterized by scanning electron microscopy, Fourier transform infrared and UV–vis spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). CEA monoclonal antibody (anti-CEA) is immobilized on AuNPs via gold-thiol chemistry to construct the electrochemical immunosensing platform, and the surface binding events between anti-CEA and CEA is achieved by monitoring the change of the electrochemical impedance response. Under optimum conditions, the modified electrode exhibits a linear impedance response to CEA in a wide range of 0.1–1000 ng mL −1 ( R 2 = 0.9959) with low detection limit of 0.06 ng mL −1 ( S/N = 3).
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Xuechun Kan,Jing Ma,Jun Ma,Dongdong Li,Fan Li,Yuyu Cao,Cheng Huang,Yan Li,Peidang Liu
Radiation therapy (RT) is one of the most effective and widely used treatment methods for glioblastoma multiforme (GBM). However, its efficacy is often compromised by the inherent radioresistance of tumor cells, while the restrictive nature of the blood-brain barrier (BBB) specifically impedes the delivery of radiosensitizer. Thus, we constructed and characterized polyethylene glycol (PEG)-functionalized silver-gold core-shell nanoparticles (PSGNPs) targeting both BBB (TfRA4) and GBM (DNA1) (TDSGNPs). Afterwards, studies conducted both in vitro and in vivo were employed to assess the BBB penetration capabilities, abilities of GBM targeting and radiosensitization effect. Transmission electron microscope images of PSGNPs showed a core-shell structure, and the results of ultraviolet-visible absorption spectroscopy and dynamic light scattering displayed that TDSGNPs were successfully constructed with excellent dispersion properties. TDSGNPs could be specifically taken up by U87MG cells and the uptake peaked at 24 h. TDSGNPs combined with RT obviously increased the apoptosis proportion of the cells. It was shown by the in vitro and in vivo investigations that TDSGNPs could target U87MG cells after crossing the BBB, and further study revealed that TDSGNPs showed an uptake peak in the tumor sites after 3 h intravenous injection. The radiosensitization of TDSGNPs was better than that of the nanoparticles modified with single aptamers and the median survival of tumor-bearing mice was greatly extended. This study demonstrated that TDSGNPs could penetrate BBB to target GBM, functioning as a promising radiosensitizer for the targeted therapy of GBM.
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高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 7
·e4829
·
IF 3.2
Juanjuan Cheng,Xinru Wei,Lun Wang,Hongqi Chen
A ratio luminescence probe was developed for detecting Staphylococcus aureus ( S. aureus ) based on luminescence energy transfer (LET) using double-wavelength emission (550 nm and 812 nm) upconversion nanoparticles (UCNPs) as donor, gold nanoparticles (AuNPs) as acceptor and the aptamer for S. aureus as the specific recognition and link unit. The LET process could cause luminescence quenching because of the spectral overlap between the acceptor and the donor at 550 nm. In the presence of S. aureus , S. aureus selectively combined with the aptamer, and the AuNPs left the surface of UCNPs, which weakened the quenching effect and restored the luminescence of UCNPs. Based on this, the ratio detection was realized by monitoring the change of the luminescence signal of the probe at 550 nm and taking the luminescence signal at 812 nm as the reference signal. Crucially, the probe has a fast reaction speed, with a reaction time of 25 min, and the detection of S. aureus is realized in the concentration range of 5.0 × 10 3 –3.0 × 10 5 CFU/ml, with the detection limit of 106 CFU/ml. Therefore, the ratio probe has great potential for detecting of S. aureus in food because of its high sensitivity, fast speed and good selectivity.
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