FOOD CHEMISTRY
·
2023
·
IF 8.8
Zihang Cheng,Nannan Li,Zhining Chen,Kaikai Li,Dongling Qiao,Siming Zhao,Binjia Zhang
High-fat diet is a risk factor for many chronic diseases, whose symptoms are probably regulated by ingesting food ingredients such as resistant starch. For cooked rice stored in cold-chain, the starch component can retrograde to generate ordered structures (helices and crystallites) and become resistant. However, the role of retrograded starch in managing hyperlipidemia symptoms is insufficiently understood. Here, compared to the normal high-fat diet, ingesting retrograded starch reduced the triglyceride and low-density lipoprotein cholesterol levels of high-fat diet mice by 17.69% and 41.33%, respectively. This relieved hyperlipidemia could be linked to the changes in intestinal bacteria. Retrograded starch intervention increased the relative abundance of Bacteroides (2.30 times higher), which produces propionic acid (increased by 8.26%). Meanwhile, Bacteroides were positively correlated with butyric acid (increased by 98.4%) with strong anti-inflammatory functions. Hence, retrograded starch intervention may regulate the body's health by altering intestinal bacteria.
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CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Mingxia Yang,Senqing Fan,Jiaojiao Chen,Yu Chen,Chuang Li,Jiaxin Meng,Haijie Qing,Yangchao Liu,Zeyi Xiao
An electrocatalytic membrane structured reactor (EMSR) fabricated by loading MnO 2 catalyst in Ti membrane pores for oxidation of n-propanol coupled with hydrogen evolution. During the process of n-propanol oxidation coupled with hydrogen evolution by MnO 2 @Ti EMSR via flow-through mode, the conversion rate of n-propanol was increased by 44%, the selectivity of propionic acid was increased by 33%, and the Faraday efficiency was increased by 59%, compared with those of MnO 2 /Ti membrane with MnO 2 immobilized on the membrane surface. Enhanced contact and mass transfer are two contributors for the catalytic performance improvement by MnO 2 @Ti EMSR via flow-through mode. Besides, the cell voltage during n-propanol oxidation coupled with hydrogen production is decreased by 305 mV with energy consumption reduced by 0.73 kWh Nm H2 -3 , compared with those of hydrogen evolution by water splitting with oxygen evolution reaction. The achievements of this work provide a promising strategy for coproduction of propionic acid and hydrogen.
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Marine Drugs
·
2023
·Vol. 21
·期 5
·265
·
IF 5.4
Sathuvan Malairaj,Suresh Veeraperumal,Wanzi Yao,Mugesh Subramanian,Karsoon Tan,Saiyi Zhong,Kit-Leong Cheong
In this study, the effects of a homogenous porphyran fromPorphyra haitanensis(PHP) on the intestinal barrier and gut microbiota were investigated. The results showed that oral administration of PHP resulted in a higher luminal moisture content and a lower pH environment for the growth of beneficial bacteria in the colon of mice. PHP significantly increased the production of total short-chain fatty acids during the fermentation process. PHP made the intestinal epithelial cells of mice arrange more tidily and tightly with a significant increase in mucosal thickness. PHP also increased the amount of mucin-producing goblet cells and the expression of mucin in the colon, which maintained the structure and function of the intestinal mucosal barrier. Moreover, PHP up-regulated the expression of tight junctions including ZO-1 and occludin, improving the intestinal physical barrier function. The results of 16S rRNA sequencing showed that PHP regulated the composition of gut microbiota in mice, increasing the richness and diversity of gut microbiota and the ratio of Firmicutes to Bacteroidetes. This study revealed that the intake of PHP is beneficial for the gastrointestinal tract and PHP could be a potential source of prebiotics in the functional food and pharmaceutical industries.Keywords:Porphyra haitanensis;porphyran;intestinal barrier;gut microbiota
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AQUACULTURE
·
2023
·
IF 4.5
Li Zhou,Fenglu Han,Kunyu Lu,Yanbing Qiao,Erchao Li
Prebiotic fermentation by gut microbiota is a key foundation for prebiotics exhibiting physiological functions. However, the relationship among prebiotic type, gut microbiota and gut microbiota metabolism remains unclear. In this study, the gut microbiota of shrimp ( Litopenaeus vannamei ) was inoculated into medium containing 1% fructooligosaccharide (FOS), galactooligosaccharide (GOS), mannanoligosaccharide (MOS), inulin, β-glucan, isomaltooligosaccharide (IMO) and xylooligosaccharide (XOS). The pH value, digestive enzyme activity, short-chain fatty acid yield and gut microbiota in the fermentation liquid were evaluated. The results showed that after 12 h of in vitro fermentation, β-glucan exhibited the lowest pH value and highest amylase activity among the other types of prebiotics. β-glucan had a higher ability to produce short-chain fatty acids (SCFA) and inhibit the formation of branched-chain fatty acids (BCFA) than the other types of prebiotics. At the genus level, β-glucan markedly promoted the growth of Bacillus . These findings suggest that β-glucan was the most suitable type of prebiotic and had greater prebiotic effects than the other types of prebiotics on the basis of in vitro fermentation by the gut microbiota of shrimp ( Litopenaeus vannamei ).
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·Vol. 140
·期 24
·e53954
·
IF 3
Jian Wang,Wen Sun,Ming He
Thermoresponsive polymer hydrogels have great potential in the fields of smart window and information encryption due to the drastic changes in transmittance caused by the phase transition at specific temperatures. The synthesis of thermoresponsive hydrogels with a wide range and sensitive temperature response is current research goal. Here, we demonstrate a polymethacrylamide (PMAAm) hydrogel with the upper critical solution temperature (UCST) ranging from 62 to −1°C by simply polymerizing the monomer in different concentrations of acetic acid (Ac) solutions. The hydrogel exhibits sensitive temperature response with a temperature hysteresis within 2°C and can maintain stable transmittance changes after 200 cycles of testing. The phase transition mechanism is revealed by combining ultraviolet visible spectra, differential scanning calorimetry, Fourier transform infrared spectra, dynamic light scattering and scanning electron microscope measurements. This thermoresponsive hydrogel can be applied in smart window and information encryption. The smart window prepared with the hydrogel had a very high Δ T lum (The luminous transmittance modulation, 94.4%), ensuring daytime illumination and privacy protection at night. Meanwhile, the smart window exhibits an excellent thermal insulation capability for energy-saving buildings. The hydrogels with different UCST are flexibly combined to display designed patterns at different temperatures, demonstrating the ability of the hydrogels to be used for information encryption.
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Food Bioscience
·
2023
·
IF 5.2
Linlin Gao,Lin Zhang,Huan Liu,Jielun Hu
In order to increase children's daily intake of dietary fiber and protein, the effects of soluble dietary fiber (SDF) supplement including high-amylose corn starch (HACS), inulin , fructo-oligosaccharides (FOS), galactose oligosaccharides (GOS), β-glucan (BG), arabinogalactan (AG), arabinoxylan (AX), and xylo-oligosaccharides (XOS) on protein digestion of multigrain noodles and the fecal microbiota of school-age children were evaluated by in vitro digestion and fermentation in this study. The addition of SDF in multigrain noodles retained a high protein content, which is beneficial for children intake. Except HACS and inulin, the addition of other SDFs significantly increased protein hydrolysis ( p < 0.05) during gastrointestinal digestion, which will enhance the protein digestion for the children. In addition, noodles (after digestion) containing SDF could reduce fermentation pH, increase short-chain fatty acid levels, and regulate microbiota composition during in vitro fermentation for 24 h. Noodles with HACS resulted in the highest short-chain fatty acids production during fermentation. Noodles containing FOS, GOS and HACS significantly increased the abundance of Bifidobacterium , Lactobacillus and Clostridium-sensu_stricto_1 ( p < 0.05) at genus level in fecal microbiota during fermentation. Results indicated that FOS, GOS and HACS are more suitable to be added to multigrain noodles to improve protein and fiber intake for school-age children.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xinmiao Wang,Qian Wang,Yuanyuan Hu,Fawen Yin,Xiaoyang Liu,Dayong Zhou
The models of rat everted gut sac and hydrolysis by rat plasma were used to clarify the hydrolysis and transport characteristics of tyrosol-SCFA esters (TYr-SEs). HPLC-UV results indicated that TYr-SEs could be hydrolyzed by intestinal lipase, which showed sustained release of SCFAs and TYr. Meanwhile, TYr-SEs and the liberated SCFAs and TYr could cross the membrane and were transported into blood circulation. TYr-SEs were further hydrolyzed by carboxylesterase in plasma. Obviously, the hydrolysis of TYr-SEs in blood also showed sustained release of SCFAs and TYr. Especially, the rates of hydrolysis and transport correlated positively with the acyl chain lengths. Besides, the above rates of the TYr-SE with a straight chain were greater than those of its isomer with a branched chain. Therefore, the above-mentioned two-step release of SCFAs and TYr clearly demonstrated that TYr-SEs would be an effective approach to enhance the beneficial health effects of SCFAs and TYr.
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JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2022
·
IF 2.345
Yu Huang,Qingfang Niu,Le Jian,Wengui Zhao,Yuan Li,Wenjuan Dong,Kai Zhang,Wenting Liang,Cheng Yang
Zirconium-porphyrin metal-organic framework (PCN-224) hybridized reduced graphene oxide (PCN-224/rGO) was coupled with gold nanoparticles (Au NPs) to form a novel nanocomposite (PCN-224/rGO@Au). The PCN-224/rGO@Au has been served as a sensing interface for electrochemical determination of Cu 2+ via differential pulse voltammetry (DPV) approach. The PCN-224 contains the active site for capturing Cu 2+ and exhibits as a biomimetic catalyst to reveal a higher catalytic performance to target Cu 2+ . Simultaneously, the rGO and Au NPs provide good electrical conductivity and efficient catalytic activity as an electrochemical indicator. Under the optimized determining conditions, the peak current of Cu 2+ was linearly proportional to the concentration over the range of 5–150 µM with the LOD of 1.5 µM (S/N = 3). This approach exhibits good sensitivity, selectivity as well as great stability, which can accurately determine the content of Cu 2+ in the real water samples.
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Foods
·
2022
·Vol. 11
·期 24
·3997
·
IF 5.561
Youyou Yang,Jing Li,Jiangtao Xing,Weihai Xing,Chaohua Tang,Zhenghua Rao,Junmin Zhang
Volatile compounds play vital roles in food sensory attributes and food quality. An analysis of volatile compounds could illustrate the sensory attributes at the microscale level. Here, untargeted profiling approaches for volatiles in five most-consumed meat species were established using headspace SPME-GC/high resolution Orbitrap MS. An extended high-resolution database of meat volatile compounds was established to enhance the qualification accuracy. Using sulfur-containing compounds, aldehydes, and ketones as the research model, the parameters including fiber coating types, extraction temperature, extraction time, and desorbing time were optimized. Principle component analysis, volcano analysis and partial least squares discriminant analysis were applied to run the classification and the selection of discriminant markers between meat varieties, respectively. Different varieties could be largely distinguished according to the volatiles’ profiles. 1-Octen-3-ol, 1-octen-3-one, 2-pentyl furan and some other furans degraded from n-6 fatty acids would contribute to distinguishing duck meat from other categories, while methyl esters mainly from oleic acid as well as dimethyl sulfoxide and carbon disulfide possibly produced from the sulfur-containing amino acids contributed to the discrimination of beef. Therefore, volatiles’ profiling not only could interpret the aroma style in meat but also could be another promising method for meat differentiation and authentication.Keywords:differentiation;GC-HRMS;SPME;statistical analysis;untargeted profiling;volatiles
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Frontiers in Microbiology
·
2023
·
IF 5.2
Zhang Fangfang,Xiao Ying,Pan Liang,Yu Ling,Liu Yanfang,Li Deshun,Liu Xiaojie
Introduction: Lyophyllum decastes (Fr.) Singer polysaccharides (LDSPs) have been verified to possess strong biological properties. However, the effects of LDSPs on intestinal microbes and their metabolites have rarely been addressed.Methods: The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.Results: The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion. After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution. The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group. Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.Discussion: These findings suggest that LDSPs might be a potential prebiotic to provide a health benefit.
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MATERIALS & DESIGN
·
2023
·
IF 8.4
Shuai Zha,Fang Yang,Zhili Ma,Huiqing Wu,Dexin Zhang,Deyuan Li
In this work, we introduce the upconverting detection nanoprobes UCNP-TPP-GOx based on the upconversion quenching effect recovery for the ultrasensitive, expeditious, quantitative, and in vitro cellular detection of glucose. This nanoplatform consists of upconversion nanoparticles (UCNP), tetraphenyl porphyrin (TPP) and glucose oxidase (GOx). In a nutshell, UCNPs core are linked with cancer-killing TPP via condensation reaction to form UCNP-TPP, subsequently, UCNP-TPP-GOx is fabricated through glucose oxidase (GOx) decoration on the surface of the UCNP-TPP. Additionally, UCNP-TPP-GOx possesses characteristic photophysical property and it is highly suitable for optical detection. Furthermore, UCNP-TPP-GOx shows excellent selectivity toward glucose after only 3-min coincubation and the limit of detection (LOD) for the glucose is calculated as low as 0.86 μg mL −1 . Moreover, the differentiation between cancer and normal cells through in vitro confocal imaging is achieved due to the large differences of glucose level in them. More importantly, the significant inhibition on carcinoma cell growth is realized by simply switching to the specific excitation wavelength, followed by the generation of toxic singlet oxygen from TPP moiety. Hence, the developed nanoprobes presenting new possibilities for on-site rapid glucose detection and concurrent theranostics in vitro in a “one stone two birds” strategy.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2022
·
IF 8.91
Guodong Chai,Dongqi Wang,Yitong Zhang,Hui Wang,Jiake Li,Xiaosheng Jing,Haiyu Meng,Zhe Wang,Yuan Guo,Chunbo Jiang,Huaien Li,Yishan Lin
Bioremediation techniques utilizing sulfate-reducing bacteria (SRB) for acid mine drainage (AMD) treatment have attracted growing attention in recent years, yet substrate bioavailability for SRB is a key factor influencing treatment effectiveness and long-term stability. This study investigated the effects of external organic substrates, including four complex organic wastes (i.e., sugarcane bagasse, straw compost, shrimp shell (SS), and crab shell (CS)) and a small-molecule organic acid (i.e., propionate), on AMD removal performance and associated microbial communities during the 30-day operation of sulfate-reducing microcosms. The results showed that the pH values increased in all five microcosms, while CS exhibited the highest neutralization ability and a maximum alkalinity generation of 1507 mg/L (as CaCO 3 ). Sulfate reduction was more effective in SS and CS microcosms, with sulfate removal efficiencies of 95.6% and 86.0%, respectively. All sulfate-reducing microcosms could remove heavy metals to different degrees, with the highest removal rate of >99.0% observed for aluminum . The removal efficiency of manganese, the most recalcitrant metal, was the highest (96%) in the CS microcosm. Correspondingly, SRB was more abundant in the CS and SS microcosms as revealed by sequencing analysis, while Desulfotomaculum was the dominant SRB in the CS microcosm, accounting for 10.8% of total effective bacterial sequences. Higher abundances of functional genes involved in fermentation and sulfur cycle were identified in CS and SS microcosms. This study suggests that complex organic wastes such as CS and SS could create and maintain preferable micro-environments for active growth and metabolism of functional microorganisms , thus offering a cost-efficient, stable, and environmental-friendly solution for AMD treatment and management.
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